Microsoft Word - jamsy2003v08n01content-arabic.doc


Agricultural and Marine Sciences, 8(1):35-40 (2003) 
© 2003 Sultan Qaboos University 
 

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Effect  of  Different Concentrations of  Growth  Regulators  on  Gardenia 
jasminoides cv. Veitchii  Micropropagation by Tissue Culture Technique 

 
G. R. Abdullah, A.A. Al-Khateeb and M. Serage 

!
ABSTRACT: Micropropagation techniques were set up for Gardenia jasminoides c.v. veitchi. Many plantlets were 
obtained by culturing shoot cuttings in MS nutrient media, 30 g/L Sucrose, 7 g/L Agar Agar, and different concentrations 
of BAP and IAA. The best concentration was 1mg /L BAP with 0.5 mg/L IAA. This concentration gave the best sprout 
growth suitable for rooting in primary and secondary culture by reculturing the stuck cutting every 6 weeks and for many 
times. We also obtained a high rooting percentage up to 98 % of natural rooting in rooting media different from 
propagation media by reducing mineral salt concentration to half, Sucrose to 20gm/L, and 2gm/L active charcoal, and 
1mg/L IAA. Plantlets were transferred to greenhous and subjected for hardening. This technique gave 22 plantlets from 
one cutting in one year.  

 
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ABDULLAH, AL-KHATEEB, AND SERAGE 
 
 

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EFFECT  OF  DIFFERENT CONCENTRATIONS OF  GROWTH  REGULATORS  
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ABDULLAH, AL-KHATEEB, AND SERAGE 
 
 

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B6&-#%&!
 
 
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Sinica  17(1):65-70. 



ABDULLAH, AL-KHATEEB, AND SERAGE 
 
 

!40

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Received November 2001. 
Accepted June 2002,