J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 161 Original Article A Checklist of Iranian Cockroaches (Blattodea) with Description of Polyphaga sp as a New Species in Iran Saedeh Sadat Hashemi-Aghdam 1, *Mohammad Ali Oshaghi 2 1Deptartment of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran 2Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran (Received 15 Apr 2013; accepted 31 May 2014) Abstract Background: Cockroaches are of vital importance medically and hygienically. They are able to contaminate foods and act as vectors of pathogenic agents such as bacteria, protozoa, and parasites to human environment either me- chanically or through their digestive system. Cockroaches belong to the phylum Arthropoda, class Insecta, and order Blattodea or Blattaria. To date, over 4,500 cockroach species have been reported from different parts of the world. We overviewed the documents involved Iranian cockroaches to up-to-date checklist of cockroach species distributed in various provinces of Iran. Methods: An extensive literature review was performed in 2013 on Iranian handbooks, reports and published data available since 1986 to obtain a comprehensive list of Iranian cockroaches. Furthermore, in an entomological survey in Tehran, cockroach specimens were collected and identified based on morphological and the DNA sequences of the mitochondrial cytochrome oxidase subunit II (COII) gene (mt-DNA COII) characteristics. Results: Morphological characterization revealed presence of an un-described species very similar to Polyphaga aegyptiaca, P. indica and somehow to Pycnoscelus surinamensis, however, supplementary molecular analysis revealed the species was associated with Polyphaga of Corydiidae (Polyphagidae). With re- gards to the report of the un-described species, the cockroach fauna of Iran includes three families, 14 genera, and 26 species. Conclusion: Some species has not been collected or reported recently and also many geographical regions of the country have not been studied yet, hence a systematic research is required to reveal the real cockroach list of the country. Geographical distributions, nomination changes, and synonyms of cockroach species are presented. Keywords: Cockroach, Blattodea, Polyphaga sp, mtDNA-COII, Molecular systematics Introduction Cockroaches are one of the oldest and the most successful insect lives have been pre- sent on the earth from Pennsylvanian Epoch (the Upper Carboniferous period). The fossil records indicate that cockroaches have un- dergone rapid changes correlated with de- velopmental cycles to obtain the ideal body form and survived as resistant taxa (Moore et al. 1952). To date, over 4,500 cockroach species have been reported from different parts of the world of which about 30 species are associated with human habitats (Valles et al. 1999). Cockroaches live all over the world but most of the species are found in tropical and equatorial regions (Schal and Hamilton 1990). Cockroaches hygienically are one of the important pests widely distributed through- out the world. Frightening and pesky appear- ance of cockroaches is another significant factor to call them pest. The sanitary and med- ical significance of cockroaches is owing to their presence in homes, groceries, food ware- houses, restaurants, hotels, hospitals on the one hand and in sewer systems and rubbish *Corresponding author: Dr Mohammad Ali Oshaghi, E-mail: moshaghi@sina.tums.ac.ir http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 162 bins on the other hand. They can act as vec- tors of pathogenic agents such as bacteria, protozoa (single-celled eukaryotes), and par- asite eggs and can transmit diseases to hu- mans either mechanically or through their digestive system. Thus they are considered a very important vector of diseases including cholera and typhoid fever to humans. More- over, cockroach infestations, including saliva, feces and cast skins, are a source of allergens and can irritate allergies and asthma in people, especially children (Farhoudi et al. 2003, Ah- med et al. 2010, Sookrung and Chaicumpa 2010, Shahraki 2011). Almost 40%–60% of patients with asthma in urban and inner-city areas possess IgE antibodies to cockroach allergens (Sohn and Kim 2012). Cockroaches belong to the order Blattodea or Blattaria (sometimes considered a suborder, Blattaria, of order Dictyoptera), and are regarded as the most primitive winged insects. Blatta is of Latin origin and means shunning light (Hanafi-Bojd and Sadaghiani 2009). Many adult cockroaches have fully devel- oped wings, whereas other cockroaches have short wings or lack wings altogether. Young, immature cockroaches resemble adults but are smaller and wingless. Cockroaches are identified by the following taxonomic fea- tures: color, number and the shape of spines on the femora, tarsus and wings, reproduc- tive organs, and head frontal. Moreover, at the posterior end of the male abdomen, there are cercus, stylus and hypandriun which are important factors in species identification. Historically, according to the classifica- tion of Mckittrick (1964) and Roth (1970), cockroaches have been regarded as a sepa- rate order divided into two superfamilies of Blattoidea and Blaberoidea which totally in- clude five families of Polyphagidae, Blaberidae, Blattellidae, Blattidae, and Cryptocercidae. However, in more recent classification, num- ber of superfamilies tops out at three includ- ing Corydioidea (Synonym: Polyphagoidea), Blattoidea (Synonym: Termitoidea), and Bla- beroidea. And number of family tops out at nine including Blaberidae, Ectobiidae (syno- nym: Blattellidae), Blattidae, Lamproblattidae, Tryonicidae, Cryptocercidae, Termitidae, Cory- diidae (Synonym: Polyphagidae), Nocticolidae (Inward et al. 2007, Lo et al. 2007). How- ever, Lo et al. (2007) criticized the classi- fication of termites as a family of Blattaria and suggested to retain the order of Isoptera (Termites with seven families including Mastotermitidae, Termopsidae, Hodotermitidae, Kalotermitidae, Serritermitidae, Rhinotermi- tidae, and Termitidae) as an unranked name within Blattaria (i.e Blattaria: Isoptera), until cockroach phylogeny is better resolved and an appropriate ranking can be applied. Cur- rently, Beccaloni and Eggleton (2013) clas- sified the termites as an epifamily (ie Ter- mitoidae including 9 families of Mastoter- mitidae, Archotermopsidae, Hodotermitidae, Stolotermitidae, Kalotermitidae, Stylotermi- tidae, Rhinotermitidae, Serritermitidae, and Termitidae) and created two other epifamilies to contain the cockroach species also placed in the superfamily Blattoidea with the ter- mites. Unfortunately, in terms of faunistic studies on the Iranian cockroaches, there are no enough records available. The only evi- dence is a paper written by Mirzayans (1986) in which four families, eleven genera and 24 species of cockroaches were reported in Iran. However, considering the report of Parcoblatta sp by Fathpour et al. (2003) and on the base of recent classification, Iranian cockroaches could be classified in three superfamilies, three families, 14 genera, and 25 species. Different species of family Blattidae can be found all over the world. Most of the spe- cies that are recognized as house pests be- long to this family. Among family Blattidae, five species have been reported from different parts of Iran. Species of family Corydiidae (Polyphagidae) live in arid regions particularly in desert and semi desert areas. In Iran twelve species of this family have already been rec- ognized. In terms of the number of species, http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 163 family Ectobiidae (Blattellidae) is the largest order of cockroaches in the world and eight species of this family have been reported from the country. The German roach, Blattella germanica, the major pest of residential dwell- ings, belongs to family Blattellidae as well. In an entomological survey, we have found two male cockroach specimens originated from north of Tehran which showed morphologi- cal characters of Corydiidae (Polyphagidae) and by some means to Pycnoscelus suri- namensis. However, further taxanomic in- vestigation revealed some morphological characters distinct the two specimens from Polyphaga aegyptiaca, P. indica, and Py. surinamensis. This encouraged us to use molecular technique to determine their iden- tity. Currently, different mitochondrial and nuclear DNA markers have been used for species identification and phylogenetic anal- ysis of different groups of cockroaches (Maekawa and Matsumoto 2000 and 2003, Maekawa et al. 2001 and 2007, Lo et al. 2006 and 2000, Park et al. 2004, Sulaiman et al. 2012). Among the loci, mitochondrial cytochrome oxidase subunit II (mtDNA- COII) gene has been commonly used for cockroaches (e.g Maekawa et al. 2007) and other insects (Liu and Beckenbach 1992, Chen et al. 2002, Oshaghi et al. 2006 and 2007, Preativatanyou et al. 2010, Chen et al. 2012). In this study we tested the mtDNA-COII sequences of the two specimens. Meanwhile, this paper overviewed the documents in- volved Iranian cockroaches to up-to-date checklist of cockroach species distributed in various provinces of the country. Materials and Methods To obtain a complete list of Iranian cock- roaches, a broad literature evaluation in- cluding Persian and English documents has been carried out on Iranian handbooks, re- ports and published data which were pub- lished since 1986 onwards. Besides, in an entomological survey in north Tehran, cockroach specimens were col- lected actively using boxes from indoor of buildings in summer 2011. The collected spec- imens were transferred to the Insect Molec- ular Laboratory of School of Public Health, Tehran University of Medical Sciences (SPH- TUMS) and then were morphologically stud- ied using the available pictorial and morpho- logical keys of Pratt and Stojanovich (1966), Mirzayans (1986), Cochran (1999), and Hanafi- Bojd and Sadaghiani (2009). Further molec- ular characters were provided to test se- quence homology and phylogenetic relation- ship with the available entries in GenBank. DNA extraction, mtDNA-COII PCR, se- quencing, and Phylogeny The male specimens were subjected to DNA extraction using two legs from each individual. Total genomic DNA was ex- tracted using QIAamp® Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol. The COII gene of the cockroach mitochondrial genome was amplified using polymerase chain reaction (PCR) and then subjected to DNA sequencing. PCR was car- ried out in 25 μL reactions, with the final concentrations of 1 out 100 μL template DNA, 1 unit (0.2 μL) of Taq polymerase, 1× PCR reaction buffer, 2mM MgCl2, 200μM of each dNTPs (Fermentas, Sinaclon) and 10 μM (1.2 μL) of each forward and reverse primer (Sinaclon, Iran). The primers used in this study were A-tLeu 5’- ATGGCAGATT AGTGCAATGG-3’ and reverse primer of B-tLys 5’-GTTTAAGAGACCAGTACTT G-3’ as previously described (Liu and Beckenbach 1992). PCR thermal program included an initial denaturation step of 94 ˚C of 5 minutes, followed by 5 cycles of 94 ˚C for 1 minute, 45 ˚C for 40 seconds, 72 ˚C for 1 minute, and then followed by 35 cycles of 94 ˚C for 40 seconds, 50 ˚C for 40 seconds, 72 ˚C for 1 minute, and a final elongation step for 72 ˚C for 5 minutes. PCR products were visu- http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 164 alized on 1% agarose gel containing ethidium bromide and using an UV transilluminator. The amplified mtDNA-COII amplicons were bidirection sequenced via the same amplifi- cation primers by Bioneer Company (S. Ko- rea) and Seqlab (Germany). The DNA se- quence reads were edited manually using the Chromas software to eliminate discrepancies between forward and reverse sequences. The consensus sequences were analyzed using the BLAST search available in Nucle- otide collection of NCBI. To determine asso- ciation of the undescribed specimens with species of Polyphagidae, most of the mtDNA- COII entries of Corydiidae (Polyphagidae) including P. aegyptiaca, Ergaula capucina, Therea sp, and Eremoblatta subdiaphana, and a subset of entries represented spe- cies of other families of the order Blattodea were retrieved from Genbank and used for data analysis. Representatives of other families were Blattella germanica, Symploce pallens, and Blattella lituricollis of Ectobiidae (Blattellidae), Shelfordella la- teralis, Blatta orientalis, Periplaneta fuliginosa, and P. americana of Blattidae, Py. suri- namensis of Blaberidae, and Locusta migratoria from Orthoptera as outgroup. The sequences were aligned using the mul- tiple alignment program CLUSTALW2 online program and manually adjusted. The two spec- imen sequences were identified to genus lev- el by sequence comparison with the GenBank entries and by the phylogenetic analysis us- ing the neighbor-joining method embedded in the CLUSTALW2. Phylogenetic analysis was performed on a combination of the data obtained from this study plus representatives of the cockroach species mentioned above. Results Entomological survey The undescribed species (Polyphaga sp) were found, out of houses close to mountain area of Assad-Abad zone, Velenjak area in northern part of Tehran. Morphological in- vestigation showed that they look like two other known Iranian species of Polyphaga indica and P. aegyptiaca (Egyptian) and by some means to Py. surinamensis but in some characters they stand as a distinct spe- cies. In addition to variation in the size and color with the mentioned species, they had a distinct pronotum with straight edge posteri- orly and laterally (Fig. 1C, 1D). Pronotum was angularly in Py. surinamensis and rounded laterally in P. aegyptiaca. They also had a separation line between inferior part of forehead and main part of face which is specific character of P. aegyptiaca but lacked distinctive ocelli (simple eyes) which is specific character of Py. surinamensis. It is noticeable that P. aegyptiaca and P. indica belong to Corydiidae (Polyphagidae) whereas Py. surinamensis belongs to Blaberidae. The most important morphologi- cal traits of this species which shared with the mentioned species include lack of strong spines along the internal margin of the mid- dle and hind femora; pronotum dark brown, except a narrow yellowish band along ante- rior and lateral margins (Fig. 1). Morpho- logical characters of the specimens somehow represented a new record for the country. Our study using molecular mtDNA COII marker revealed that the specimens belong to Polyphaga of Corydiidae (Polypha- gidae) however their COII sequences were only 85% homologue to P. aegyptiaca and far more different from other species of Corydiidae (Polyphagidae) indicating pre- sumably a distinct and new species in Iran. The mtDNA-COII sequences of the two specimens were submitted to GenBank with accession numbers of KJ769153-KJ769154. The sequences include 683 bp un-ambiguous data and there were no polymorphic sites between the samples. Interestingly, align- ment of the sequence data showed a fixed six DNA base pairs deletion equals to two amino acids (AA) deletion in species of http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 165 Corydiidae (Polyphagidae) including the specimens found in this study in comparison with other cockroaches (Fig. 2) indicating the undescribed specimens definitely belongs to Corydiidae (Polyphagidae) and presuma- bly to Polyphaga genus. Further phylogenetic analysis confirmed the close association of the undescribed specimens to the Ployphaga genus. Phylogenetic analysis was performed using 16 sequences of the mtDNA-COII of various cockroach species and of Locusta migratoria (Orthoptera) as outgroup. The cladogram inferred using the neighbor-join- ing method strongly supported association of the specimens (we call them as Polyphaga sp) with other Corydiidae (Polyphagidae) species (Fig. 3). The tree resolved very well the four families of the cockroaches each one formed a major group. Two families of Blaberidae and Ectobiidae (Blattellidae) were shown to be sister groups. Checklist of Iranian Cockroaches With regards to the report of the un- described species (Polyphaga sp), the cock- roach fauna of Iran includes three families, 14 genera, and 26 species (Table 1). Superfamily Blattoidea Family Blattidae Periplaneta americana (Linnaeus 1758) Synonymy: Blatta aurelianensis Fourcroy 1785, Periplaneta americana colorata Rehn 1902, Blatta domicola Risso 1826, Blatta ferrugineofusca Gronovius 1764, Blatta heros Eschscholtz 1822, Blatta kakkerlac De Geer 1773, Blatta americana Linnaeus 1758, Blatta orientalis Sulzer 1776, Blatta siccifolia Stoll 1813, Periplaneta stolida Walker 1868. Iranian records: Almost all parts of Iran (Mirzayans 1986), Southwest Iran (Shahraki 2010), Hamedan-Hamedan (Salehzadeh 1995, Mahjoob et al. 2010, Nejati et al. 2011), Is- fahan (Fathpour et al. 2003), Isfahan-Kashan (Doroodgar et al. 2006), Khuzestan-Khorram- shahr (Kassiri and Kazemi 2012), Kohkiluyeh va Boyer-Ahmad-Yasuj (Shahraki et al. 2011), Kurdestan-Sanandaj (Vahabi et al. 2007), Mazandaran-Sari (Motavali Haghi et al. 1997), Tehran (Karimi Zarchi and Vatani 2009), Zanjan-Zanjan (Mohammadi 1996), Qom- Qom (Fahiminia et al. 2010). Periplaneta australasiae (Fabricius 1775) Synonymy: Blatta aurantiaca Stoll 1813, Blatta australasiae Fabricius 1775, Blatta domingensis Palisot de Beauvois 1805, Periplaneta emittens Walker 1871, Periplaneta inclusa Walker 1868, Periplaneta repanda Walker 1868, Periplaneta subcincta Walker 1868, Polyzosteria subornata Walker 1871, Periplaneta zonata Haan 1842. Iranian records: Most southern states and warm (Persian Gulf coast) (Mirzayans 1986), Hamedan-Hamedan (Mahjoob et al. 2010, Nejati et al. 2011), Khorassan-e-Jonubi- Birjand, Yazd (Hanafi-Bojd and Sadaghiani 2009). Blatta orientalis (Linnaeus 1758) Synonymy: Blatta badia Saussure 1863, Blatta castanea Blanchard 1851, Blatta culinaris De Geer 1773, Blatta europaea Bartsch 1846, Blatta ferruginea Thunberg 1810, Stylopyga orientalis gracilis Adelung 1903, Blatta hemialata Gistel 1856, Pulex imperator Westwood 1858, Blatta lucifuga Poda 1761, Kakerlac pallipes Philippi 1863, Kakerlac platystetho Philippi 1863, Blatta secunda Schaeffer 1769, Stylopyga orientalis spontanea Semenov-Tian-Shansky 1909, Blatta tertia Schaeffer 1769. Iranian records: Almost all parts of Iran (Mirzayans 1986), Southwest Iran (Shahraki et al. 2010), Hamedan-Hamedan (Salehzadeh 1995, Mahjoob et al. 2010, Nejati et al. 2011), Tehran (Karimi Zarchi and Vatani 2009), Zanjan-Zanjan (Mohammadi 1996). Shelfordella lateralis (Walker 1868) Synonymy: Blatta lateralis Walker 1868, Shelfordella ahngeri Adelung 1910, http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 166 Paraloboptera sillemi Hanitsch 1935, Periplaneta tartara Saussure 1874, Periplaneta lateralis Walker 1868, Shelfordella tartara zarudnyi Adelung 1910. Iranian records: Sistan va Balouchestan (Sarbaz, Saravan, Jalgh, Pishin, Zabol), Ar- debil-Mogan, Kermanshah-Kangavar, Isfahan- Kashan, Tehran (Tehran, Varamin), Fars (Firuzabad, Kakan), Golestan (Gonbad-e- Kavus), Kerman-Kerman, Khuzestan (Masjed- Soleiman, Ahvaz), Hormozgan (Bandar- Lengeh, Issin), (Mirzayans 1986), Southwest Iran (Shahraki et al. 2010), Isfahan (Fathpour et al. 2003), Hamedan-Hamedan (Salehzadeh 1995), Kohkiluyeh va Boyer-Ahmad-Yasuj (Shahraki 2011), Yazd (Hanafi-Bojd and Sadaghiani 2009), Gilan Province, Assalem district (Authors of this article). Shelfordella monochroma (Walker 1871) Synonymy: Blatta monochroma Walker 1871, Shelfordella gussakovskii Bei-Bienko 1950, Shelfordella tertia Bei-Bienko 1938, Periplaneta monochroma Walker 1871. Iranian record: Hormozgan-Bandar-Ab- bas (Mirzayans 1986). Superfamily Corydioidea (Polyphagoidea) Family Corydiidae (Polyphagidae) Polyphaga aegyptiaca (Linnaeus 1758) Synonymy: Blatta lata Gistel 1856, Blatta omega Costa 1839, Heterogamia punctata Fischer 1846, Blatta sphaerica Gistel 1856, Blatta spinifer Kirby 1910, Heterogamia spinipes Fischer 1846, Steleopyga trichoprocta Fischer 1833, Blatta aegyptiaca Linnaeus 1758. Iranian records: Khuzestan (Ahvaz, Dezful), Fars (Shiraz, Firuzabad, Dehram), Lorestan (Aligudarz-Ghalikuh 2360 m. alt.), Tehran (Damavand 2070 m. alt.), Mazandaran (Babol, Gaemshahr), Golestan-Kalaleh, Kermanshah (Gilan-e-Gharb, Chuar, Sumar, Ghasre-Shirin), Elam-Mehran, (Mirzayans 1986), Isfahan (Fathpour et al. 2003), Khu- zestan-Ahvaz (Vazirianzadeh et al. 2009). Polyphaga indica (Walker 1868) Synonymy: There is no synonym. Iranian records: Khuzestan-Ahvaz, Fars (Mount Dena 2210 m. alt. Western slope, Kazerun: Gavkoshak, Dashte-Arjan 1900 m. alt.), Azarbayjan-e-Sharghi (Tabria, Moghan), Khorassan-e-Razavi- Safi Abad, Sistan va Balouchestan, Tehran (Varamin, Tehran, Ru- dehen, Damavand), Isfahan-Golpayegan, Semnan-Garmsar, Salt desert, Kerman-Ru- dbar, Mazandaran (Mirzayans 1986). Polyphaga saussurei (Dohrn 1888) Synonymy: Heterogamia saussurei Dohrn 1888, Polyphaga camelorum Kirby 1903, Heterogamia pellucida Redtenbacher 1889. Iranian records: Khorassan-e-Razavi (Dareh-Gaz), Hormozgan-Minab, Fars (Midast, Firuzabad, Farrashband, Kazerun, Sarmash- had), Sistan va Balouchestan (Zabol, Pishin, Bampur, Iranshahr, Khash, Sarbaz-Dez) (Mirzayans 1986), Yazd (Hanafi-Bojd and Sadaghiani 2009). Note: In the study of Salehzadeh (1995), one unknown species of this genus has been reported from Hamedan. Polyphaga sp (sp Nov?) In this study for the first time two specimens of a new but undescribed species (Polyphaga sp) (sp Nov?) were found in a summer time (Agust 2011), in a house (third floor) where was built in close affinity with Touchal Mountain, Asad-abad zone, Shemi- ranat, northern Tehran. The vocher specimen (Fig. 1) is deposited in Entomology Meu- seum of the School of Public Health, Tehran University of Medical Sciences. Arenivaga livida (Brunner von Wattenwyl 1865) Synonymy: There is no synonym. Iranian records: Azarbayjan-e-Sharghi (Jolfa, Aras) (Bey-Bienko 1950), Azarbayjan- e-Sharghi (Tabriz-Khalat pushan) (Mirzayans 1986). http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 167 Hemelytroblatta subhyalina (Chopard 1921) Synonymy: Arenivaga subhyalina Chopard 1921, Polyphaga subhyalina Chopard 1921, Iranian records: Gazvin (Chopard 1929, Bey- Bienko 1950), North of Iran (Bey-Bienko 1950), Fars (Shiraz, Dashte-Arjan 1900 m. alt.) (Mirzayans 1986). Hemelytroblatta roseni (Brancsik 1898) Synonymy: Arenivaga roseni Brancsik 1898, Homoeogamia roseni Brancsik, 1898. Iranian records: Sistan va Balouchestan- Bampur, Isfehan-Murchekhort, Khuzestan (Chopard 1929, Bey-Bienko 1950), Isfahan (Kashan, Kabutarabad, Barzok), Semnan- Garmsar, Sistan va Balouchestan (Zabol, Khash), Tehran-Varamin (Mirzayans 1986), Isfahan (Fathpour et al. 2003). Hemelytroblatta longipes (Chopard 1929) Synonymy: Heterogamodes longipes Chopard 1929, Arenivaga longipes Chopard 1929. Iranian records: North of Iran (Chopard 1929, Bey-Bienko 1950), Fars (Firuzabad, Shiraz, Dehram), Semnan-Garmsar, Tehran- Varamin (Mirzayans 1986). Hemelytroblatta marismortui (Janson 1891) Synonymy: Arenivaga marismortui Jan- son 1891, Heterogamia maris-mortui Janson 1891. Iranian records: Fars (Kazerun-Chah Chenar), probably in provinces Lorestan, Azarbayjan-e-Gharbhi, Kermanshah, Khuzes- tan (Mirzayans 1986). Hemelytroblatta latifrons (Chopard 1929) Synonymy: Heterogamodes latifrons Chopard 1929, Arenivage latifrons Chopard 1929. Iranian records: Boushehr (Chopard 1929), Chaharmahal va Bakhtiari (Bakhtiari Moun- tains) (Bey-Bienko 1950), Khuzestan (Ahvas- Molla Sani) (Mirzayans 1986). Heterogamisca persica (Chopard 1921) Synonymy: Polyphaga persica Chopard 1921. Iranian records: Markazi-Arak, Sistan va Balouchestan (Khash-Mount Taftan), Kho- rassan-e-Jonubi-Nehbandan (Chopard 1929), Kerman-Esfandagheh, Isfahan (Kabutarabad), Sistan va Balouchestan (Chahbahar, Tiss, Iranshahr), Gazvin, Yazd, Khuzestan (Ahvaz), Fars-Yazd khast (Mirzayans 1986). Leiopteroblatta monodi (Chopard 1969) Synonymy: Does not exist. Iranian record: Lut-Desert (Mirzayans 1986). Tivia inconspicua (Bei-Bienko 1950) Synonymy: There is no synonym. Iranian records: Sistan va Balouchestan (Bahukelat, Ghasr-Ghand, Dashtiari, Tang Sarheh) (Bey-Bienko 1950), Hormozgan (Isin: Mount Geno 750 m. alt.) (Mirzayans 1986). Superfamily Blaberoidea Family Ectobiidae Blattella germanica (Linnaeus 1767) Synonymy: Blatta asiatica Pallas 1773, Blatta bivittata Serville 1838, Eublattella cuneivittata Hanitsch, 1925, Phyllodromia cuneivittata Hanitsch 1925, Blatta daurica Laxmann 1769, Phyllodromia magna Tepper 1895, Phyllodromia niitakana Shiraki 1931, Blatta obliquata Daldorf 1794, Ischnoptera paralella Tepper 1893, Blatta germanica Lin- naeus 1767, Ischnoptera parallela Princis 1969, Blattella germanica shuguroffi Karny 1908, Blatta transfuga Brünnich 1763. Iranian records: Almost all parts of Iran (Mirzayans 1986), Southwest Iran (Shahraki et al. 2010), Fars (Fallahzadeh et al. 2008), Hamedan-Hamedan (Salehzadeh 1995, Mah- joob et al. 2010, Nejati et al. 2011), Hor- mozgan-Bandar-Abbas (Shahi et al. 2008), Isfahan (Fathpour et al. 2003), Isfahan-Kashan http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 168 (Doroodgar et al. 2006), Kerman-Kerman (Kamyabi et al. 2006), Kermanshah (Limoee et al. 2011), Kordestan-Sanandaj (Vahabi et al. 2007), Kohkiluyeh va Boyer-Ahmad- Yasuj (Shahraki et al. 2011), Mazandaran- Sari (Motavali Haghi et al. 1997, Abedi and Dayer 2006, Enayati and Haghai 2007), Khorassan-e-Razavi-Mashhad (Naderinasab and Moravvej 2012), Tehran (Ladonni 1997, Mousavi et al. 2004, Baniardalani et al. 2006, limoee et al. 2006, Nasirian 2007, Karimi Zarchi and vatani 2009, Fakoorziba et al. 2010), Zanjan-Zanjan (Mohammadi 1996, Ghavami et al. 2005), Qom-Qom (Fahiminia et al. 2010). Blattella biligata (Walker 1868) Synonymy: Blatta cercalis Walker 1868, Ischnoptera evansi Chopard 1921, Blatta fasciceps Walker 1871, Ischnoptera fulvastra Chopard 1924, Blatta mellea Krauss 1902, Ischnoptera modestiformis Karny 1915, Symploce mossambica Princis 1965, Symploce persica Bei-Bienko 1935, Ischnoptera biligata Walker 1868, Phyllodromia unicolor Brunner von Wattenwyl 1893, Blatta cercalis Walker 1868. Iranian records: Sistan va Balouchestan (Bey-Bienko 1950), Hormozgan (Bandar- Abbas, Shamil, Minab, Tonbe bozorg), Sistan va Balouchestan (Sarbaz, Saravan, Iranshahr, Chabahar, Tiss, Baftan) (Mir- zayans 1986). Note: In the studies of Salehzadehs (1995), two unknown species of Symploce have been reported from Hamedan. Supella (Supella) longipalpa (Fabricius 1798) Synonymy: Blatta cubensis Saussure 1862, Blatta extenuata Walker 1868, Blatta incisa Walker 1868, Blatta phalerata Saussure 1863, Ischnoptera quadriplaga Walker 1868, Blatta subfasciata Walker 1871, Blatta (Phyllodromia) supellectilium Serville 1838, Blatta transversalis Walker 1871, Ischnoptera vacillans Walker 1868, Blatta longipalpa Fabricius 1798. Iranian records: Tehran (Bey-Bienko 1950), Isfahan (Fathpour et al. 2003), Southwest Iran (Shahraki et al. 2010), Hamedan-Hamedan (Salehzadeh 1995). Symploce zarudniana (Bei-Bienko 1963) Synonymy: There is no synonym. Iranian records: Sistan va Balouchestan (Bey-Bienko 1950), Hormozgan (Bandar- Abbas, Shamil), Sistan va Balouchestan (Pishin, Iranshahr, Baftan), Fars (Mirzayans 1986). Balta ramifera (Walker 1871) Synonym: Blatta ramifera Walker 1871. Iranian record: Sistan va Balouchestan- Bampur (Bey-Bienko 1950), Sistan va Balouchestan- Baftan (Mirzayans 1986). Phyllodromica (Phyllodromica) polita (Krauss 1888) Synonym: Aphlebia polita Krauss 1888. Iranian record: Gilan (Assalem, Parehsar 750m. alt) (Mirzayans 1986). Phyllodromica (Lobolampra) persa (Bei- Bienko 1935) Synonymy: Does not exist. Iranian record: Golestan (Gorgan-Shahkuh) (Bey-Bienko 1950). Parcoblatta sp Hebard 1917 Synonym: Ischnoptera Fox 1917, Plata- modes Scudder 1862. Iranian records: Isfahan (Fathpour et al. 2003). This species was reported in a study that was focused on cockroaches as reser- voirs and vectors of drug resistant Salmo- nella spp Since this species was new for Iran, further investigation will confirm its presence in the country. http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 169 Fig. 2. Partial Comparison of the mtDNA-COII gene of different cockroach specie. The dashes (-) in the red square in the last three lines show the six DNA base pairs deletion in species of Polyphaga genus including the undescribed specimens (Polyphaga sp) and other two Polypahaga aegyptica from Japan (J) and London (L). The species from top to down respectively are: Ergaula capucina, Blattella lituricollis, Blattella germanica, Symploce pallens, Pycnoscelus surinamensis, Blatta orientalis, Periplaneta americana, Periplaneta fuliginosa, Shelfordella lateralis, Eremoblatta subdiaphana, and Polypahaga aegyptica. Fig. 3. The neighbor-joining tree for mtDNA-COII sequences of some representative species of four known cock- roach families. Except for Polyphaga sp, sequence data of other species were retrieved from Genbank. The line un- derneath shows the genetic distance scale http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 170 Table 1. Classification of the 26 Iranian cockroach species. NS: Not stated Superfamily Family Genus Species General name Blattoidea Blattidae Periplaneta P. americana American Periplaneta P. australasiae Australian Blatta B. orientalis Oriental Shelfordella S. lateralis Turkistan Shelfordella S. monochroma NS Corydioidea Corydiidae (Polyphagidae) Tivia T. inconspicua NS Polyphaga Polyphaga P. aegyptiaca P. indica Egyptian NS Polyphaga P. saussurei NS Polyphaga Polyphaga sp unknown Hemelytroblatta H. livida NS Hemelytroblatta H. subhyalina NS Hemelytroblatta H. longipes NS Hemelytroblatta H. roseni NS Hemelytroblatta H. marismortui NS Hemelytroblatta H. latifrons NS Heterogamisca H. persica NS Leiopteroblatta L. monodi NS Blaberoidea Ectobiidae (Blattellidae) Supella S. longipalpa Brown-banded Symploce S. zarudniana NS Blattella B. biligata NS Blattella B. germanica German Balta B. ramifera NS Phyllodromica P. polita NS Phyllodromica P. persa NS Parcoblatta P. sp Wood Fig. 1. Photos of an adult male specimen of Polyphaga sp found in Assad-Abad, northern Tehran, Iran, 2011. General top (A and B) and ventral (C) views, pronotum with a narrow yellowish band along anterior and lateral margins and straight posteriorly (D), and end of abdomen with a pair of anal styles (E). (Photos: original) Discussion Result of this study revealed presence of an undescribed species of Polyphaga in Iran. In this research we tried to use the cockroach morphological characteristics for identifica- tion of the unknown specimens but presence of some similar characteristics among three species of P. indica, P. aegyptiaca, and Py. surinamensis made identification problem- atic. This situation highlights usefulness of molecular data in resolving systematic of close- ly related species. The molecular analysis in- cluding phylogenetic analysis using mtDNA COII sequences showed that the specimens belong to Corydiidae (Polyphagidae) and sug- gested that they are most closely related to Polyphaga genus. MtDNA COII is the most frequently gene used for molecular system- http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 171 atic of cockroaches (Liu and Beckenbach 1992, Park et al 2004, Lo et al 2000 and 2006, Bergamaschi et al. 2007, Maekawa et al. 2007), however, due to lack of available data on dif- ferent Polyphaga species in GenBank data- base, identification of the specimens at the species level postpones until other Polyphaga species molecular data be- comes available. Also more specimens especially female specimens of the species should be collected and subjected to further morphological, biological and ecological investigations. With regards to the report of the un- described species, the cockroach fauna of Iran includes three families, 14 genera, and 26 species. However, it should be mentioned that since some species has not been col- lected or reported recently and many geo- graphical regions of the country have not been studied yet, hence a systematic research is required to reveal the real cockroach list of the country. Conclusion The usefulness of molecular markers for species identification. Recently regions of mtDNA have been used as DNA barcodes to identify and classify living organisms. Ap- plication of DNA barcode could put light on many un-identified or questionable taxa. Due to lack systematic study on cockroach fauna of Iran, available data is not representing real fauna of the Iranian cockroaches. It is highly recommended to conduct a comprehensive systematic research using a combination of morphological, ecological, and molecular tech- niques in various geographical regions to re- veal the composition of Iranian cockroach species. Acknowledgements This work was financially supported by Tehran University of Medical Sciences. The authors declare that they have no conflicts of interest. The authors declare that there is no conflict of interests. References Abedi A, Dayer MS (2006) Evaluation of the effect of the fungus Metarhizium anisopliae, as a biological control agent, on German cockroaches Blattella germanica. J Med Sci Pathobiol. 8(1): 31–36 [In Persian]. Ahmed A, Minhas K, Sahar NE, Aftab O, Sher-Khan F (2010) In silico identifi- cation of potential American cockroach (Periplaneta americana) allergens. Iran J Publ Health. 39(3): 109–115. Baniardalani M, Nourjah N, Changani A (2006) Susceptibility of cockroaches to Lambadacyhalothrin and Cypermethrin in hospitals of Tehran University of Medical Science. Qazvin Univ Med Sci J. 10(1): 95–100 [In Persian]. Beccaloni G, Eggleton P (2013) Order Blattodea. Zootaxa. 3703(1): 046–048. Bergamaschi S, Dawes-Gromadzki TZ, Scali V, Marini M, Mantovani B (2007) Kary- ology, mitochondrial DNA and the phylogeny of Australian termites. Chromosome Res. 15(6): 735–753. Bey-Bienko G YA (1950) Fauna of the USSR Insects, Blattodea. Institute of Zoology, Academy of Sciences, Moscow, (in Russian). Chen R, Jiang L-Y, Qiao G-X (2012) The effectiveness of three regions in mito- chondrial genome for aphid DNA bar- coding: A case in Lachininae. PLoS ONE 7(10): e46190. Chen B, Harbach RE, Butlin RK (2002) Mo- lecular and morphological studies on the Anopheles minimus group of mosquitoes in southern China: taxonomic review, distribution and malaria vector status. Med Vet Entomol. 16(3): 253–265. http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 172 Chopard L (1929) Orthoptera Paleartica V11, Les Polyphagiens de la faune Palearctique (Orth., Blatt.). Eos. 5: 223–357. Cochran DG (1999) World Health Organi- zation, Communicable diseases preven- tion and control [and] WHO pesticide evaluation scheme (WHOPES), p. 83. Cochran DG (1999) Cockroaches: biology, distribution, and control. Geneva, World Health Organization, Document No: WHO/CDS/CPC/WHOPES/99.3. Doroodgar A, Arbabi M, Asadi MA (2006) Survey on parasitic and fungal agents of hospital cockroaches in Kashan- 2002. J Kashan Univ Med Sci Feyz. 10(1): 28–35 [In Persian]. Enayati AA, Motavali-Haghi F (2007) Bio- chemistry of pyrethroid resistance in German cockroach (Dictyoptera, Bla- tellidae) from hospitals of Sari, Iran. Iran Biomed J. 11(4): 251–258. Fahiminia M, Paksa A, Zarei A, Shams M, Fazlzadeh-davil M, Bakhtiari H, Norouzi M (2010) Survey of optimal methods for the control of cockroaches in sew- ers of Qom City. Iran J Health Envi- ron. 3(1): 19–26 [In Persian]. Fakoorziba MR, Eghbal F, Hassanzadeh J, Moemenbellah-fard MD (2010) Cock- roaches (Periplaneta americana and Blattella germanica) as potential vec- tors of the pathogenic bacterial found in nosocomial infections. Ann Trop Med Parasitol. 104(6): 521–528. Fallahzadeh M, Asadi R, Talebi AA (2008) The first record of Anastatus (Anastatus) tenuipes (Hym: Eupelmidae), a parasi- toid of Blattella germanica (Blattaria: Blattellidae) in Iran. J Entomol Soc Iran. 28: 99–100. Farhoudi A, Pourpak Z, Mesdaghi1 M, Kazemnejad A, Chavoshzadeh Z (2003) The study of cockroach allergy in Ira- nian children with asthma. Acta Med Iran. 41(3): 150–155. Fathpour H, Emtiazi G, Ghasemi E (2003) Cockroaches as reservoirs and vectors of drug resistant Salmonella spp. Iran Biomed J. 7(1): 35–38. Ghavami MB, Mohammadi J, Ladonni H (2005) Susceptibility of Blattella germanica (Blattaria: Blattidae) to permethrin in Shahid Beheshti hospi- tal, Zanjan. J Zanjan Univ Med Sci Health. 13: 8–16 [In Persian]. Hanafi-Bojd AA, Sadaghiani Sh (2009) Cockroaches of Iran. Tehran Univer- sity of Medical Sciences Press, Tehran [In Persian]. Inward G, Beccaloni G, Eggleton P (2007) Death of an order: a comprehensive molecular phylogenetic study confirms that termites are eusocial cockroaches. Biol Lett. 3: 331–335. Kamyabi F, Vatandoost H, Aboulhasani M, Aghasi M, Telmadarraiy Z, Abaei MR (2006) Sensitivity level of German cockroach (Blattella germanica) to three pirimphos methyl, lambdacyhalothrin and propoxur insecticides in three Ker- man hospitals. Maz Univ Med Sci J. 16(52): 98–108 [In Persian]. Karimizarchi AA, Vatani H (2009) A survey on species and prevalence rate of bac- terial agents isolated from cockroaches in three hospitals. Vector Borne Zoon- otic Dis. 9(2): 197–200. Kassiri H, Kazemi S (2012) Cockroaches [Periplaneta americana (L.), Dictyop- tera, Blattidae] as carriers of bacterial pathogens, Khorramshahr County, Iran. Jundishapur J Microbiol. 5(1): 320–322. Ladonni H (1997) Susceptibility of different field strains of Blattella germanica for pyrethroides (Orthoptera: Blattellidea). Iran J Publ Health. 26(3–4): 35–40. Limoee M, Enayati AA, Ladonni H, Vatan- doost H, Baseri H, Oshaghi MA (2006) Various mechanisms responsible for permethrin metabolic resistance in seven field-collected strains of the German http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 173 cockroach from Iran, Blattella germanica (L.) (Dictyoptera: Blattellidae). Pestic Biochem Physiol. 87:138–146. Limoee M, Enayati AA, Khassi K, Salimi M, Ladonni H (2011) Insecticide re- sistance and synergism of three field collected strains of the German cock- roach Blattella germanica (L.) (Dictyop- tera: Blattellidae) from hospitals in Kermanshah. Iran Trop Biomed. 28(1): 111–118. Liu H, Beckenbach AT (1992) Evolution of the mitochondrial cytochrome oxidase II gene among 10 orders of insects. Mol Phylogenet Evol. 1(1): 41–52. Lo N, Engel MS, Cameron S, Nalepa CA, Tokuda G, Grimaldi D, Kitade O, Krishna K, Klass K-D, Maekawa K, Miura T, Thompson GJ (2007) Save Isoptera: A comment on Inward et al. Biol Lett. 3(5): 562–563. Lo N, Luykx P, Santoni R, Beninati T, Bandi C, Casiraghi M, Lu WH, Zakharov EV, Nalepa CA (2006) Molecular phylog- eny of Cryptocercus wood-roaches based on mitochondrial COII and 16S sequences, and chromosome numbers in Palearctic representatives. Zoolog Sci. 23(4): 393–398. Lo N, Tokuda G, Watanabe H, Rose H, Slaytor M, Maekawa K, Bandi C, Noda H (2000) Evidence from multiple gene sequences indicates that termites evolved from wood-feeding cock- roaches. Curr Biol. 10(13): 801–804. Maekawa K, Kon M, Araya K, Matsumoto T (2001) Phylogeny and biogeography of wood-feeding cockroaches, genus Salganea Stål (Blaberidae: Panesthiinae), in Southeast Asia based on mitochon- drial DNA sequences. J Mol Evol. 53(6): 651–659. Maekawa K, Matsumoto T (2000) Molecular phylogeny of cockroaches (Blattaria) based on mitochondrial COII gene se- quences. Syst Entomol. 25(4): 511–519. Maekawa K, Matsumoto T (2003) Bio- geography of Japanese wood-feeding cockroaches genus Salganea and Panesthia (Blaberidae: Panesthiinae). Mol Phylogenet Evol. 27(1): 156–159. Maekawa K, Kon M, Matsumoto T, Kitade O, Araya K (2007) Phylogeography of the asian wood-feeding cockroach salganea raggei roth (Blattaria: Blaberidae) based on the mitochon- drial coii gene. Oriental Insects. 41: 317–325. Mahjoob M, Nejati J, Keyhani A (2010) Evaluation of bacterial infection of ex- ternal surface and digestive system of cockroach species. Med J Hormozgan Univ. 14(1): 80–86 [In Persian]. McKittrick FA (1964) Evolutionary studies of cockroaches. Cornell Univ AgrExp Sta Mem. 389: 1–197. Mirzayans H (1986) Fauna of Iranian cock- roaches (Orthopteroidea: Blattaria). J Entomol Soc Iran. Suppl 4. p. 134 [In Persian]. Mohammadi J (1998) Determining the Ac- tive Genera of Cockroaches living in the hospitals and residential houses of Zanjan; their seasonal activities, and bacterial pollutions. First Congress on Iran Medical Entomology, Pasteur In- stitute, Tehran, Iran. p. 139. Moore RC, Lalicker CG, Fisher AC (1952) Invertebrate fossile. McGraw-Hill, N Y P. 571. Motavali-Haghi F, Gholami SH, Sedaghat MM (1997) Survey of susceptibility of cockroaches to insecticides in Sari hospitals in 1996. Maz Univ Med Sci J. 7(16): 1–6 [In Persian]. Mousavi SB, Vatandoost H, Rafinejad J (2004) Evaluation of the resistance level of different strains of german cockroach, Blattella germanica (L.), to delta-methrin, propoxur and pirimiphos-methyl insec- ticides in tehran, 2000. J Med Sci Pathobiol. 7(1–2): 97–106 [In Persian]. http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 174 Naderinasab M, Moravvej GH (2012) Bacte- rial and fungal contamination of cock- roaches at two seasons in public habit- ants of Mashhad, Iran. The 13th Ira- nian and the Second International Con- gress of Microbiology, 2012 July 14– 16, Ardabil University of Medical Sci- ences, Ardabil, Iran, p. 81. Nasirian H, (2007) Duration of fipronil and imidacloprid gel baits toxicity against Blattella germanica strains of Iran. Iran J Arthropod-Borne Dis. 1(2): 40–47. Nejati J, Keyhani A, Moosa-Kazemi SS, Mohammadi M, Mahjoob M, Boostan- bakhsh A (2011) Cockroaches’ bacte- rial infections in wards of hospitals, Hamedan City, west of Iran. Asian Pac J Trop Dis. 2(5): 381–384. Oshaghi MA, Shemshad Kh, Yaghobi-Ershadi MR, Pedram M, Vatandoost H, Abaie MR, Akbarzadeh K, Mohtarami F (2007) Genetic structure of the malaria vector Anopheles superpictus in Iran us- ing mitochondrial cytochrome oxidase (COI and COII) and morphologic mark- ers: a new species complex? Acta Trop. 101(3): 241–248. Oshaghi MA, Yaaghoobi F, Abaie MR (2006) Pattern of mitochondrial DNA variation between and within Anoph- eles stephensi (Diptera: Culicidae) bi- ological forms suggests extensive gene flow. Acta Trop. 99(2–3): 226–233. Park YC, Maekawa K, Matsumoto T, Santoni R, Choe JC (2004) Molecular phylog- eny and biogeography of the Korean woodroaches Cryptocercus spp. Mol Phylogenet Evol. 30(2): 450–464. Pratt HD, Stojanovich CJ (1966) Cock- roaches: Key to some species found in the United States. US Department of Health, Education and Welfare, Public Health Service, CDC Atlanta. Preativatanyou K, Sirisup N, Payungporn S, Poovorawan Y, Thavara U, Tawatsin A, Sungpradit S, Siriyasatien P (2010) Mitochondrial DNA-based identifica- tion of some forensically important blowflies in Thailand. Forensic Sci Int. 10, 202(1–3): 97–101. Roth LM (1970) Evolution and taxonomic significance of reproduction in Blattaria. Ann Entomol. 15: 75–96. Salehzadeh A (1995) Study on Blattaria in- sects and determination of their sus- ceptibility to ficam and Lindane in Hamedan. J Hamadan Univ Med Sci Health. 2(2): 32–36 [In Persian]. Schal C, Hamilton, RL (1990) Integrated sup- pression of synanthropic cockroaches. Annu. Rev. Entomol. 35: 521–551. Shahi M, Hanafi-Bojd AA, Vatandoost H (2008) Evaluation of five local for- mulated insecticides against German cockroach (Blattella germanica L.) in Southern Iran. Iran J Arthropod-Borne Dis. 2(1): 21–27. Shahraki GH, Noor HM, Rafinejad J, Shahar MK, Bin-Ibrahim Y (2010) Biorational control programme for the German cockroach (Blattaria: Blattellidae) in selected urban communities. Trop Bi- omed. 27(2): 226–235. Shahraki GH, Noor HM, Rafinejad J, Shahard MK, Bin-Ibrahim Y (2011) Field efficacy of siege gel bait in an IPM program on life stages of German cockroach (Blataria, Blattellidae) in a residential building. Asian Biomed. 5(5): 619–624. Sohn MH, Kim KE (2012) The cockroach and allergic diseases. Allergy Asthma Immunol Res. 4(5): 264–269. Sookrung N, Chaicumpa W (2010) A revisit to cockroach allergens. Asian Pac J Allergy Immunol. 28(2–3): 95–106. Sulaiman IM, Anderson M, Khristova M, Tang K, Sulaiman N, Phifer E, Simp- son S, Kerdahi K (2011) Development of a PCR-restriction fragment length polymorphism protocol for rapid de- tection and differentiation of four http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 161–175 SS Hashemi-Aghdam and MA Oshaghi: A Checklist … 175 cockroach vectors (group I "Dirty 22" species) responsible for food contami- nation and spreading of foodborne pathogens: public health importance. J Food Prot. 74(11): 1883–1890. Vahabi A, Rafinejad J, Mohammadi P, Biglarian F (2007) Regional evaluation of bacterial contamination in hospital environment cockroaches. Iran J Envi- ron Health Sci Eng. 4(1): 57–60. Valles SM, Koehler PG, Brenner RJ (1999) Comparative insecticide susceptibility and detoxification enzyme activities among pestiferous blattodea. Comp Infibous Biochem Physiol C Pharmacol Toxicol Endocrinol. 124(3): 227–232. Vazirianzadeh B, Mehdinejad M, Dehghani R (2011) Identification of bacteria which possible transmitted by Polyphaga aegyptica (Blattodea: Blattidae) in the region of Ahvaz, SW Iran. Jundishapur J Microbiol. 2(1): 36–40. http://jad.tums.ac.ir Published Online: March 11, 2015