J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 226 Original Article Laboratory Evaluations of the Fractions Efficacy of Annona senegalensis (Annonaceae) Leaf Extract on Immature Stage Development of Malarial and Filarial Mosquito Vectors *Younoussa Lame 1, Elias Nchiwan Nukenine 1, Danga Yinyang Simon Pierre 1, Ajaegbu Eze Elijah 2, Charles Okechukwu Esimone 3 1Department of Biological Sciences, University of Ngaoundere, Cameroon 2Department of Pharmaceutical and Medicinal Chemistry, Nnamdi Azikiwe University, Nigeria 3Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Nigeria (Received 12 Jan 2014; accepted 9 July 2014) Abstract Background: Within the framework to control mosquitoes, ovicidal, larvicidal and pupicidal activity of Annona senegalensis leaf extract and its 4 fractions against Anopheles gambiae and Culex quinquefasciatus were evaluated in the laboratory conditions. Methods: Ovicidal test was performed by submitting at least 100 eggs of mosquitoes to 125, 250, 500, 1000 and 2000 ppm concentrations, while larvicidal and pupicidal effects were assessed by submitting 25 larvae or pupae to the concentrations of 2500, 1250, 625 and 312.5 ppm of plant extract or fractions of A. senegalensis. Results: The eggs of An. gambiae were most affected by N-hexane (0.00% hatchability) and chloroform (03.67% hatchability) fractions compared to Cx. quinquefasciatus where at least 25 % hatchability were recorded at 2000 ppm. For larvicidal test, N-hexane (LC50= 298.8 ppm) and chloroform (LC50= 418.3 ppm) fractions were more effective than other fractions on An. gambiae larvae while, a moderate effectiveness was also observed with N- hexane (LC50= 2087.6 ppm), chloroform (LC50= 9010.1 ppm) fractions on Cx. quinquefasciatus larvae. The highest mortality percent of the pupae were also recorded with N-hexane and chloroform fractions on An. gambiae at 2500 ppm. As for Cx. quinquefasciatus only 50 % and 36 % mortality were recorded with N-hexane and chloroform fractions respectively. Conclusion: The extract of A. senegalensis was toxic on immature stage of mosquito species tested. By splitting methanolic crude extract, only N-hexane and chloroform fractions were revealed to possess a mosquitocidal effects and could be considered and utilized for future immature mosquito vectors control. Keywords: Fractions, Pupicidal, Annona senegalensis, Anopheles gambiae, Culex quinquefasciatus Introduction Apart nuisances they inflict to human beings, mosquitoes are responsible of dread- ful diseases such as malaria, filariasis, den- gue haemorrhagic fever, etc widespread in the world (Murugan et al. 2007, Kamaraj et al. 2009). Most of Sub-Saharan Africa coun- tries have stable endemic malaria because climatic conditions, which are ideal for the transmission, coincide with the range of An. gambiae, An. arabiensis and An. funestus, the most efficient vector mosquitoes in the world (Foko et al. 2011). In Cameroon An. gambiae is the principal vector of malaria in rural and urban areas (Foko et al. 2011). Filariasis, a disease affecting the arms, legs and genitals, is much prevalent in the world. Filariasis caused by Wuchereria bancrofti is transmitted by Cx. quinquefasciatus, mos- quitoes widespread in the countries now and lymphatic filariasis infects 80 million people annually of which 30 million cases exist in chronic infection (Samidurai et al. 2009). Synthetic pesticides have been extensively used for vector control by either killing, pre- *Corresponding author: Dr Younoussa Lame, E -mail: youlasobi@yahoo.fr http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 227 venting adult mosquitoes to bite human be- ings or by killing mosquito immature stages at the breeding sites of the vectors (Joshep et al. 2004). Development of insect resistance to synthetic pesticides such as Malathion, DDT, Deltamethrin and even biopesticides such as Bacillus thuringiensis (Tabashnik 1994), added to high operational cost, envi- ronmental pollution and deleterious effects on non-target organisms are the problems people are facing to control vector borne dis- eases (Mittal et al. 2003). These problems have highlighted and need for the development of new strategies for se- lective mosquito control. With these prob- lems in focus, it becomes increasingly neces- sary to search for an alternative in the de- velopment of environmentally safe, bio- degradable, low cost, target specific insecti- cides for mosquito control and which can be used with minimum care by individuals and communities in specific situations (Dua et al. 2010). Plants may be a source of alternative agent to replace the synthetic insecticides for mosquito control. Toxicity of phytochemicals in mosquitoes was first reported by Camp- bell et al. (1933). Review papers from all over the world have documented the toxic effect of plant extracts on mosquito eggs, larvae and pupae (Tare et al. 2004, De Lima et al. 2006, Promsiri et al. 2006, Govindarajan et al. 2012, Dhivya and Manimegalai 2013). Earlier, the plant extracts belonging to the fam- ily of Annonaceae such as Annona muricita, A. cherimolia, A. squamosa, Cananga odorata, Ferula hormonis, etc have shown larvicidal effect against Anopheles sp and Culex quinque- fasciatus (Saxena et al. 1993, Bodadilla- Alvarez et al. 2002, Moore and Lenglet 2004, Isman 2006). Das et al. (2007) reported that the ethanol leaf extract of Annona squamosa was found to have the most promising lar- vicidal activity against Cx. quinquefasciatus larvae. Ovicidal, larvicidal and pupicidal ef- fects of Hyptis suaveolens, Calotropis gigantea and Delonix elata against An. gambiae and Cx. quinquefasciatus were also reported (Ivoke et al. 2009, Govindarajan et al. 2012, Dhivya and Manimegalai 2013). Gueye et al. (2011) reported insecticidal activity of extract A. senegalensis extract on eggs and adults groundnut weevils Caryedon serratus. In northern part of Cameroon, the leaves of this plant are used locally to protect maize, millet and sorghum against weevils’ attacks (Ngamo et al. 2007). In ethno-medicine, the leaves of A. senegalensis are used as antidrepanocitory and antitrypanosomic (Ogbadoyi 2007), antidiarrheic (Suleiman 2008), cures snake bite, generalized eodemes, aches and constipation (Akoegninou et al. 2006). With a large scale of activity as insecti- cide and medicinal plant, it should be advis- able to extent biological properties of this plant on mosquitoes. This study was aimed to evaluate ovicidal, larvicidal and pupacidal activity of A. senegalensis methanolic crude extract and its fractions (N-hexane, chloro- form, ethyl-acetate and methanol fractions) on An. gambiae and Cx. quinquefasciatus mosquito species. Materials and Methods Collection and processing the plant material The fresh leaves of A. senegalensis were collected at Dang, a village of Ngaoundere in the Adamaoua region (latitude 7°24.949’N, longitude 13°32.870’E and altitude 1093 masl), Cameroon in December 2011. The plant was identified by the herbalist, Pr. Mapongmetsem Pierre-Marie, Department of Biological Science, University of Ngaoundere, Cameroon and then confirmed at the Na- tional Herbarium in Yaoundé, where vouch- er sample was deposed. The leaves are then dried at room temperature, then grounded with an electric grinder and then stored at 4 oC in the refrigerator until use. http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 228 Extraction and fractionation The method of Gueye et al. (2011) was followed for the purpose. Indeed, five hun- dred (500) grams of powder were macerated in 2500 ml of methanol for 72 h at room temperature and then the maceration was filtrated using filter paper Whatman No.1. The filtrate was summited to Rotary Evapo- rator apparatus to obtain a residue called crude extract. Part of this crude extract was separated successively by the method of dif- ferential solubility in four solvents of differ- ent polarity: n-hexane, chloroform, ethyl-ac- etate and methanol. The crude extract was mixed with silica gel (70–260 mesh size) and macerated in N-hexane, then filtered with Whatman No. 1 filter paper after phase sepa- ration. N-hexane fraction and marceration (1) are recovered. Marceration (1) is dried in the open air and then soaked in chloroform, phase chloroform fraction filtrated and marceration (2) are also recovered. Marceration (2) after dried in open air is soaked in ethyl-acetate; phase ethyl-acetate fraction filtered and marceration (3) are also recovered. Marceration (3) is finally taken up in methanol to recover the polar compounds in the methanol frac- tion after filtration. Each fraction has been concentrated using Rotary evaporator and the solid fractions gotten were stored at -4 oC until bioassays. Mosquito breeding The larvae of Cx. quinquefasciatus were collected from the laboratory of National Arbovirus Research Center (NAVRC), Enu- gu, Nigeria in February 2013 while An. gambiae larvae were collected from stagnant water in the gutter at Awka market, Anambra State, Nigeria (06°12'23"N, 07°03'23"E) and identified also in NAVRC in January 2013. Larvae were kept in plastic trays containing tap water. Larvae were fed a diet containing crayfish and biscuit in a ratio of 3: 1, respec- tively. Pupae were transferred from the trays to a cup containing tap water and were main- tained in our insectary cage (45×45×40 cm), where adults emerged. Adults were maintained in cages and were continuously provided with 10 % sucrose solution in a jar with a cotton wick. On day five, blood meal was given to the female adults for eggs maturation. Beaker with 100 ml of tap water lined with filter paper was kept inside the cage for oviposition (Kamaraj et al. 2008). All the experiments were carried out, at 27±2 °C and 75–85 % relative humidity under 12: 12 light and dark cycles. Ovicidal activity For ovicidal activity, the method of Ku- mar et al. (2012) was followed for the pur- pose. The freshly laid eggs were collected by providing ovitraps in mosquito cages kept 2 days after the female mosquitoes were given a blood meal. Indeed, 100 gravids female mosquito were placed in a screen cage where ten oviposition cups were introduced for oviposition, 30 min before the start of the dusk period. The eggs were laid on filter pa- per No. 1 provided in the ovitrap. Out of these ten cups, nine were filled with test so- lution of 125, 250, 500, 1000 and 2000 ppm concentration of plant extract/ fractions of the leaves of A. senegalensis while one was filled with 99 ml of distilled water mixed with 1 ml of Tween-80 used as a control. A minimum of 100 eggs was used for each treatment, and the experiment was replicated four times. After treatment, the eggs were sieved through muslin cloth, thoroughly rinsed with tap water, and left in plastic cups filled with tap water for hatching assessment after counting the eggs under microscope (Su and Mulla 1998). The percent of egg mortality was calculated on the basis of nonhatchability of eggs with unopened opercula (Chenniappan and Kadarkarai 2008). Larvicidal test The larvicidal activity of extract/fractions http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 229 of A. senegalensis was evaluated against An. gambiae and Cx. quinquefasciatus according to the method described by WHO (1996). The extract/fractions were dissolved in 0.5 ml of Tween-80. The concentrations of 2500, 1250, 625 and 312.5 ppm of extract/fractions were prepared in the volume of 100 ml with tap water in the 250 ml beakers. Twenty five fourth instar larvae were transferred into the used and four replicates were maintained for each concentration. Mortality was recorded after 24 h of exposure, during which no food was given to the larvae. Larvae were consid- ered dead if appendages did not move when probed with needle in the siphon or cervical region. Larvae incapable of rising to the sur- face or not showing the characteristic diving reaction when water was disturbed, were considered moribund and added to the dead larvae for calculating percentage of mortal- ity. Data were adjusted for control mortality using Abbott’s formula (Abbott 1925), if mortality in the control sets exceeded 5 %. Pupicidal effect Pupicidal effect was assessed according to the method applied by Ashfaq and Ashfaq (2012). Twenty five freshly emerged pupae of each mosquito species were transferred into beakers of 250 ml volume, containing 75 ml of tap water. The extract/fractions of the plant used were dissolved in Tween-80 and then added with tap water to make up to 100 ml corresponding to the concentrations of 2500, 1250, 625 and 312.5 ppm of extract/ fractions of A. senegalensis were made. Each treatment was replicated four times and the number of emerged adults for each repli- cation was recorded after 48 h. Phytochemical screening The qualitative phytochemical analyses of the components responsible of toxicity on in- sects were carried out according to the meth- ods of Harborne (1973) and Trease and Evans (1989). These methods are founded on de- tection the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tan- nins, phenolic compounds, steroids, terpenoids, oil and fats which possess insecticidal prop- erties in the extract and fractions of A. senegalensis. Statistical analysis The values recorded from toxicity essays were transformed in percentage of mortality, hatchability using Microsoft Excel 2010 and were corrected using Abbot’s formula when- ever required. The percentage of mortality, hatchability data were subjected to the ANOVA procedure using the Statistical Package for the Social Science (SPSS 16.0). Duncan test at P= 0.05 was applied for mean separation. Probit analysis (Finney 1971, SPSS 16.0) was applied to determine lethal concentrations causing 50 % (LC50) and 90 % (LC90) mortality of larvae and pupae 24h after treatment application. Results The results of ovicidal activity of A. senegalensis extracts against against An. gambiae and Cx. quinquefasciatus are pre- sented in Table1. A significant (P< 0.001) variation of percentage of eggs hatchability is observed in mosquito species assessed. The rate of hatchability has significantly (P< 0.001) reduced with the increasing of con- centration. In comparison with the methanolic crude extract, the fractionation process pointed out the effectiveness of N-hexane followed by chloroform fractions on mosquito eggs. At the highest concentration (2000 ppm), less than 5 % rate of hatchability of An. gambiae were recorded with N-hexane (0.00%), Methanolic crude extract (04.67%), chloroform fraction (03.67%) contrary to ethyl-acetate and methanol fractions where, the high values of 22.67 % and 60.33% were recorded respectively. The eggs of Cx. http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 230 quiquefasciatus were less affected by the extracts used. The percentage of hatchability was 100 % with Methanol fraction with all concentrations tested and at least, 25 % of eggs hatched were observed in others extract or fractions of A. senegalensis. The toxicities of the fractions of A. senegalensis extract on early four instar An. gambiae and Cx. quinquefasciatus larvae were noted, and the LC50, LC90, 95 % confi- dence limits of LCL and UCL and chi- square were also calculated (Table 2). At 2500 ppm and 1250 ppm, 100 % mortality of the larvae of An. gambiae was recorded with N-hexane and chloroform fractions. Among the fractions, N-hexane (LC50= 298.8 ppm, LC90= 572.9 ppm) and chloroform (LC50= 418.3 ppm, LC90= 822.0 ppm) fractions were found to be more effective than other frac- tions against An. gambiae larvae. As for Cx. quinquefasciatus, a moderate effectiveness was also observed with the mortality percent of 48% for N-hexane fraction, 29.33 % for methanolic crude Extract and 13.33 % for chloroform fraction recorded at 2500 ppm. In comparison of fractions, N-hexane frac- tion (LC50= 2087.6 ppm) was most toxic followed by methanolic crude extract (LC50= 5884.1 ppm) and chloroform fraction (LC50= 9010.1 ppm) while, no mortality was recorded with Ethyl-acetate and methanol fractions. Apart ethyl-acetate and methanol fractions of A. senegalensis, others fractions especially N- hexane and chloroform fractions possessed a significant (P< 0.001) efficacy against pu- pae of mosquito species assessed. At the highest concentration (2500 ppm), 80 %, 70 % and 62 % mortality of An. gambiae pupae were recorded with N- hexane, chloroform fractions and methanolic crude extract re- spectively (Fig. 1). A moderate mortality of Cx. quinquefasciatus pupae were noted with N-hexane fraction (50%) chloroform frac- tion (36%) and methanolic crude extract (34%) at the highest concentration (2500 ppm) (Fig. 2). At all concentrations were tested and no mortality of the pupae of An. gambiae and Cx. quinquefasciatus was rec- orded with ethyl-acetate and methanol frac- tions of A. senegalensis. The fractions of A. senegalensis leaves extract were screened for the presence of major phytochemical groups responsible of insecticidal activity. The preliminary phyto- chemical screening of the crude extract re- vealed the presence of alkaloids, flavonoids, saponins, tannins, phenolic compounds, ster- oids, terpenoids, oil and fats except steroids (Table 3). By splitting the crude extract, the same phytochemicals were found in N-hex- ane fraction excepted saponins, steroids and terpenoids. Alkaloids, flavonoids tannins and phenolic compounds were also found in chlo- roform fraction. Table 1. Hatchability percent of Anopheles gambiae and Culex quinquefasciatus eggs treated with extract/fractions of Annona senegalensis Mosquito species Conc (ppm) Percentage of egg hatch ability Fractions MCE NHF CHF EAF MTF F Anopheles gambiae 0 100±0.00fA 100±0.00fA 100±0.00fA 100±0.00eA 100±0.00eA 0.00ns 125 69.67±1.53eB 62.67±2.08eA 79.00±2.00eC 96.33±1.53eD 98.67±0.58eD 285.59*** 250 52.00±2.00dB 39.67±2.08dA 58.33±2.08dC 81.33±2.08dD 95.67±1.15dE 421.70*** 500 34.00±1.00cB 19.67±3.21cA 29.67±1.53cB 65.0±3.61cC 88.67±1.53cD 423.60*** 1000 17.67±1.53bC 04.00±2.00bA 10.00±2.00bB 41.33±2.08bD 77.00±2.00bE 716.29*** 2000 04.67±1.53aA 00.00±0.00aA 03.67±1.15aA 22.67±3.06aB 60.33±2.08aC 545.37*** F 1850*** 1153*** 1689*** 516.18*** 348.42*** Culex quinquefas- ciatus 0 100±0.00eA 100±0.00fA 100±0.00fA 100±0.00eA 100±0.00aA 0.00ns 125 98.00±1.00eB 88.00±2.00eA 96.67±1.53eB 100.0±0.00eC 100.0±0.00aC 50.63*** 250 92.67±1.53dC 74.00±2.00dA 84.67±1.53dB 98.00±1.00dD 100.0±0.00aD 176.69*** http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 231 500 81.67±3.51cC 58.33±1.53cA 69.67±1.53cB 95.67±0.58cD 100.0±0.00aD 263.67*** 1000 66.67±2.08bC 42.67±3.06bA 55.67±0.58bB 90.33±0.58bD 100.0±0.00aE 594.27*** 2000 57.00±3.00aC 25.33±2.08aA 40.67±2.52aB 84.67±1.53aD 100.0±0.00aE 644.53*** F 192.67*** 592.68*** 729.05*** 168.93*** 0.00ns MCE: Methanolic Crude Extract, NHF: N-hexane fraction, CHF: Chloroform fraction, EAF: Ethyl-Acetate fraction, MTF: Methanol fraction Means ± SE in the same column for the same category of concentration, followed by the same small letter and in the same row for the same category of extract, followed by the same capital letter do not differ significantly at P= 0.05 (Duncan‘s test). Each datum represents the mean of three replicates of 100 eggs each. ns= P> 0.05, ***= P< 0.001 Table 2. LC50 and LC90values [ppm (95% fiducial limits)] at 24 h of fractions of Annona senegalensis extract against fourth instar larvae of Anopheles gambiae and Culex quinquefasciatus MCE= Methanolic crude extract, NHF= N-hexane fraction, CHF= Chloroform fraction, EAF= Ethyl-Acetate frac- tion, MTF: Methanol fraction, FL= Fiducial Limit, LC= Lethal concentration, NS= No significant. *= P< 0.01, variances and covariances have been multiplied by the heterogeneity factor in computing fiducial limits of lethal concentrations because the probability of χ2 value P< 0.05, - = undetermined values because of low or no mortality Table 3. Qualitative phytochemical screening of some components of extract/fractions of Annona senegalensis Photochemical Components Annona senegalensis MCE NHF CHF EAF MTF Alkaloids + + + _ _ Flavonoids + + + + _ Saponins + _ _ + + Tannins + + + + + Phenolic compounds + + + _ _ Steroids _ _ _ _ _ Fats and oils + + _ _ + Terpenoids + _ _ + + MCE= Methanolic crude extract, NHF= N-hexane fraction, CHF= Chloroform fraction, EAF= Ethyl-acetate fraction, MTF= Methanol fraction, +=present, - = absent Mosquito species Extracts Slope±SE R2 LC50 (95% FL) LC90 (95% FL) χ 2 An. gambiae MCE 2.69±0.14 0.98 973.3 (840.9-1132.7) 2914.8 (2290.3-4130.2) 33.44* NHF 4.54±0.38 0.77 298.8 (274.6-320.1) 572.9 (530.6-629.0) 8.63NS CHF 4.37±0.27 0.85 418.3 (394.3-441.9) 822.0 (760.6-902.6) 16.19NS EAF 2.38±0.18 0.97 2789.3 (2464.3-3256.2) 9662.1 (7427.4-13697.9) 17.31NS MTF 2.45±0.46 0.70 8511.4 (5608.7-20304.7) 28360.3 (13685.4-133304.7) 15.37NS Cx. quinquefasciatus MCE 1.64±0.17 0.94 5884.1 (3888.6-12610.7) 35659.9 (15583.6-175387.5) 19.36* NHF 1.68±0.14 0.98 2807.6 (2382.6-3467.3) 16240.9 (11105.9-27263.4) 14.01NS CHF 1.95±0.28 0.88 9010.1 (6024.9-18200.9) 40915.2 (19789.1-149218.8) 16.05NS EAF - - - - - MTF - - - - - Table 1. Continued… http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 232 Fig. 1. Pupicidal activity of extract/fractions of Annona senegalensis against Anopheles gambiae Fig. 2. Pupicidal activity of extract/fractions of Annona senegalensis against Culex quinquefasciatus Discussion Overall, ovicidal, larvicidal and pupicidal activity of various extract or fractions of the leaves of A. senegalensis were demonstrated on An. gambiae and Cx. quinquefasciatus mosquito species. Thus, toxicity of fractions varied with solvent used. N-hexane and Chloroform fractions showed promising mosquitocidal activity. Indeed, plants are extensively reported to possess toxic effect on the mosquitoes and can be utilized as a potent source of mosquito control (Kumar et al. 2012). The secondary metabolites are known to be effective against a wide range of insect pests as well as mos- quito vectors (Sriwattanarungsee et al. 2008). These compounds may jointly or independent- ly prove its efficacy against the mosquito targets by its ovicidal, larvicidal, pupicidal, adulticidal and by inhibition of growth ac- tivity (Borah et al. 2010). Earlier, some plants such as Annona squamosa L., Gloriosa superba L., Millingtonia hortensis, Abuta grandifolia, Minthostachys setose, Azadirachta indica, Ocimum gratissimum and Hyptis suaveolens, etc have been reported to control mosquito population (Ciccia et al. 2000, Kaushik and Saini 2008, Bagavan et al. 2009, Okigbo et al. 2010). The ovicidal effects were generally dose dependent. Rajkumar and Jebanesan (2004) recorded similar observations in their study of ovicidal activity of Moschosma polystachyum leaf extract against Cx. quinquefasciatus. Com- pared to the present study, a complete inhibi- tion of egg hatching of An. gambiae with methanolic extract of Hyptis suaveolens was reported by Ivoke et al. (2009). The ethanolic flower extract Calotropis gigantea has ex- hibited toxic effect on egg rafts of Cx. quinquefasciatus with 100 percent mortality at 200 ppm (Dhivya and Manimegalai 2013). Govindarajan et al. (2011) recorded similar findings in which the methanol leaf extract of Coccinia indica exerted zero hatchability (100% mortality) at 150 ppm for Cx. quinque- fasciatus. No hatchability eggs of Ae. aegypti were also recorded with methanol, benzene and acetone extracts of Cassia fistula at 160 mg/l (Govindarajan 2009). On the contrary of this study, ovicidal effect of A. senegalensis was obtained with ethyl-acetate and methanol fractions on the eggs of Caryedon serratus (Gueye et al. 2011). This difference can be explained by the difference on insect species used and the phytochemical constituents of MCE NHF CHF EAF MTF Extract/ fractions MCE NHF CHF EAF MTF Extract/ fractions % m o rt al it y % m o rt al it y Culex quinquefasciatus Anopheles gambiae 100.00 80.00 60.00 40.00 20.00 0.00 100.00 80.00 60.00 40.00 20.00 0.00 http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 233 plant extracts and extraction solvent used (Shalaby et al. 1998). In comparison to this study, the chloro- form soluble fractions of Tagetes erecta showed the highest toxicity than the other samples and consequently, the lowest LC50 values (75.48 mg/L) in fourth instar larvae of Cx. quinquefasciatus (Farjana et al. 2011). The hexane extracts of Cleistanthus collinus and Murraya koeingii plants showed 100 percent mortality at 24 h bioassay against the third instar larvae of Cx. quinquefasciatus at 1000 ppm concentration (Tennyson et al. 2012). A significant mortality of four instar larvae of Cx. quinquefasciatus was also rec- orded with methanol extract of Cliona celata with LC50= 95.63 ppm and LC90= 242.16 ppm (Appadurai et al. 2013). The results are also comparable with an earlier report by Fred-Jaiyesimi and Anthony (2011) reported that the effects of the methanol extract, pe- troleum ether and chloroform fractions of Paullinia pinnata leaf have been investi- gated against the third and fourth instar lar- vae of An. gambiae. Govindarajan et al. (2008) reported also that methanolic leaf extract of Acalypha indica was more lethal to the egg and larvae of Cx. quinquefasciatus and An. stephensi. Kamaraj et al. 2011 re- ported values at 1000 ppm of 78, 73, 75 and 100 % mortality larvae of An. subpictus with N-hexane, chloroform, ethyl-acetate and meth- anol bark extracts of Annona squamosa re- spectively. Anupam et al. (2012) reported that N-hexane is the most non polar (polarity index of 0.1) that mainly extracts essential oil, chloroform or ethyl acetate are moder- ately polar (polarity index of 4.1) that main- ly extracts steroids, alkaloids, etc. The pres- ence of these phytochemicals distributed in each fraction confers to the fraction, its larvicidal property. Prabhu and Murugan (2011) observed a significant pupicidal effect of plant extracts of Moringa oleifera against An. stephensi and pupal mortality of greater than 70 % was encountered, similar to the results of the pre- sent study with An. gambiae. The pupae of Ae. aegypti were found greatly susceptible to higher dose (230 ppm) of plant extract Catharanthus roseus which caused mortality of 79 % (Remia and Logaswamy 2010) con- firms the findings of the present study with N-hexane fraction on An. gambiae. The ef- fectiveness of the extract or fractions of A. senegalensis could be explained by the pres- ence of alkaloids and others compounds which are toxics for eggs, larvae and pupae of mos- quito species. Earlier, Jolad et al. (1984) re- ported the presence of alkaloids, carbohy- drate, lipids, amino acids, polyphenols, es- sential oils terpenoids the genus Annona sp plant. Conclusion Annona senegalensis contained active ovicidal, larvicidal and pupicidal compound in its leaves. However, these phytochemical compounds found in N-hexane and chloro- form fractions could be the key candidates in insecticide values of this plant. This makes it a more suitable candidate for the development of new potential eco-friendly insecticides. Further investigation is needed to identify the active compounds of N-hexane and chlo- roform fractions responsible for its activity. Acknowledgements The authors are grateful to the help of the botanist, Pr Mapongmetsem PM of the De- partment of Biological Sciences, University of Ngaoundere, Cameroon, for identification of the plant. The staff of Faculty of Pharma- ceutical Sciences, Nnamdi Azikiwe Univer- sity, Anambra State, Nigeria where the la- boratory experiments were carried out is highly acknowledged for their kind collabo- rations. We are also grateful to the labora- tory of National Arbovirus Research Center http://jad.tums.ac.ir Published Online: March 11, 2015 J Arthropod-Borne Dis, December 2015, 9(2): 226–237 Y Lame et al.: Laboratory Evaluations of … 234 (NAVRC), Enugu, Nigeria for the mosquito species supplied and identification. No fund was received for conducting this study. The authors declare that there is no conflict of interests. 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