J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 282 http://jad.tums.ac.ir Published Online: January 06, 2016 Original Article Immunization of Cattle with Tick Salivary Gland Extracts *Ali Nikpay 1, Sedigheh Nabian 2 1Department of Pathobiology, Amol University of Special Modern Technologies, Amol, Iran 2Department of Parasitology, Faculty of Veterinary Medicine, Tehran, Iran (Received 15 Oct 2014; accepted 25 Feb 2015) Abstract Background: Rhipicephalus (Boophilus) annulatus tick is one of the most important ectoparasite of cattle. Recently, several laboratories in the world have been concentrated on immunizing cattle against tick using various types of tissue extracts of ticks. The aim of this study was to evaluate the effect of immunization of cattle with tick salivary gland extract on biological parameters of ticks and humoral immune responses of cattle. Methods: Fourteen more dominant protein bands identified as immunogenic by Western-blot analysis were eluted from polyacrylamide gel. Test and control groups were injected three times with eluted proteins and sterile PBS (pH= 7.2) respectively with equivalent amount of adjuvant. After four weeks a tick challenge was performed. Fi- nally, biological parameters of collected engorged female ticks were recorded and humoral immune responses to immunization measured by ELISA. Results: The results indicated immunization of cattle resulted in reduction in mean tick counts, attachment, en- gorgement weights, feeding index, egg mass weight, hatchability and fertility index (respectively 63.1%, 62.6%, 30.2%, 36.4%, 40%, 78.7% and 13.3%) and increased duration of feeding, pre-oviposition and incubation period of eggs (respectively 8.6%, 45 and 31.34%). All changes were statistically significant (P< 0.05). Results showed an increase in antibody production of test group from the first week after immunization. The antibody level was boosted following tick infestation. Conclusion: This investigation indicates that immunization of cattle with these antigens could induce a protective immune response against Rh. (B.) annulatus tick that would be expected to provide a safe non-chemical means of tick control. Keywords: Rhipicephalus (Boophilus) annulatus, Immunization, Salivary gland, Humoral immune responses, Tick Introduction Rhipicephalus (Boophilus) annulatus tick is one of the most important ectoparasites of cattle that can occasionally complete its life cycle successfully on sheep, goats and wild ungulates such as antelopes. Surviving in Asia, Africa, America and Europe, this spe- cies of tick causes economic losses due to direct effects on the preferred hosts and by the pathogens they transmit (Murrell and Barker 2003, Estrada-peña et al. 2004, Rajput et al. 2006, Wikel 1999a, b). Therefore, tick control is a continuing global priority. Traditional control methods such as us- ing chemicals have had partially successful results, however, acaricide compounds are expensive and have adverse effects such as a high incidence of resistance among tick pop- ulations, as well as harmful effects on verte- brate hosts, human beings and the environ- ment. These increasing problems have stim- ulated researches on alternative methods of tick control in recent years (Mattewson et al. 1975, Walker 1994, Nari and Hansen 1999, FAO 2004, Rajput et al. 2006). These meth- ods include biological control methods such as pasture spelling and artificial selection for tick- resistant cattle. Such steps can reduce tick burdens, but enhancement of host re- sistance through artificial immunization with a vaccine against ticks would constitute a *Corresponding author: Dr Ali Nikpay, E-mail: ali.nikpay@gmail.com J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 283 http://jad.tums.ac.ir Published Online: January 06, 2016 major advance in control process. Several attempts have been done to immunize ac- tively cattle against ticks and it has been re- ported that protective host immune re- sponses can damage female tick internal or- gans and interfere with tick's feeding and fertility behavior (Wikel 1999a, b, Willadsen and Jongejan 1999). Performing various functions in the life cycle of Ixodid ticks especially in their feeding procedure, the salivary glands are the major route for pathogen transmission. To facilitate attachment and engorgement, ticks produce biologically active compo- nents with anti-haemostatic, anti-inflamma- tory and immunomodulatory effects. “The initial secretion of the tick saliva contains a cement-like material, which helps the tick to establish a firm attachment with the host tissue” (Hager and Burgess 1980). Exposure to proteins secreted by ticks can provoke antigen-specific humoral and cel- lular responses in the mammalian hosts. Containing the natural antigens injected by the tick during infestation, salivary gland extracts might be an obvious choice to in- duce immunity against ticks (Willadsen 1980, Brossard and Fivaz 1982, Wikel 1996). The purpose of this research was to de- termine the protein pattern of Rh. (B.) an- nulatus salivary glands by SDS-PAGE and to investigate the effects of immunizing cat- tle with immunogenic fractions of salivary gland extract of this tick against tick infes- tation. The quality of immune responses measured by ELISA and the efficacy of im- munization was evaluated by analyzing feed- ing and fecundity parameters of ticks har- vested from treatment group compared to the control groups. Materials and Methods Ticks Engorged female Rh. (B.) annulatus ticks were collected from healthy cattle (free of any transmissible pathogens), washed with 70% ethanol and then washed three times with sterile phosphate buffer saline (PBS) pH= 7.2, after drying they were kept under a constant temperature of 28 ºC, a relative humidity of 85% and a 12:12 (L: D) photo period. Experimental animal Eleven healthy Holstein calves (3–5 months old) were purchased from a tick-free area (Tehran Veterinary Medicine Faculty Re- search Institute) and kept in tick-proof pens. Antigen preparation Semi engorged adult female ticks fed for about 7–10 days on tick-bite free cattle, was collected to rear and maintain clean ticks. After washing with 70% ethanol and sterile PBS, ticks were opened along their dorsal surface as previously described (Brown et al. 1984). Then, salivary glands were removed, dissected free of other tissues, placed into PBS containing 1 mM phenyl methyl sulphonyl fluoride (PMSF) at 4 ºC. Salivary glands were suspended in 200 μl PBS with PMSF, grinded by glass beads, sonicated (Dr Hielscher GmbH, Germany) for 30 minutes with 0.4 s cycles (40W) on ice and completely homog- enized by freeze-defreeze alternatively. The mixture then were centrifuged at 12000×g for 15 minutes at 4 ºC , supernatant were collected and stored at -20 ºC until use. The protein concentration of salivary gland ex- tract determined according to the method of Warburg (Hudson and Hay 1984, Tietz and Andresen 1986). Protein Electrophoresis Protein analysis by SDS-PAGE was car- ried out using a discontinuous gel system (Laemmli 1970) with an acrylamide con- centration of 5% in stacking gel and 12% in the separation gel. A high molecular weight protein ladder (Fermentas, SMO661) was used as molecular weight markers. J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 284 http://jad.tums.ac.ir Published Online: January 06, 2016 Western blot analysis The protein bands from polyacrylamide gel were electro blotted to a nitrocellulose paper (NCP) using a mini blotter (BioRad) according to Wang's method (Wang and Nuttall 1994). Non- specific reactive sites on the NCP blocked for 1 h in Tris-buffered sa- line with 0.5% tween 20 (TBS-T pH 8), containing 3% bovine serum albumin (HAB- tech) on a shaking plate at room tempera- ture. The NCP was incubated for 1 h at 37 ºC with bovine sera diluted 1:50 in PBS (pH= 7.2), The NCP was incubated for a further 1 h at 37 ºC with sheep anti- bovine IgG (Serotech, conjugated with HRPO) di- luted 1:2000 in PBS-tween, washed and re- acted with Di amino benzidine (DAB) (Sigma) as a substrate. The NCP was washed 3 times between each step of the assay with PBS-tween. Finally, the immunogenic pro- tein fractions were appeared on the NCP. Protein extraction from polyacrylamide gel After staining the polyacrylamide gel with Coomassie blue, the desired bands identified by western blotting were cut out with a scalpel blade, sliced to a minimum and put into a microcentrifuge tube. Gel slices were washed twice with distilled water and soaked in 1 mM 2-Mercaptoethanol for 15 min. Then, gel slices were incubated in elution buffer for 12 hours. After centrifuge, the supernatant was decanted and replaced by elution buffer and incubated for 4 hours. The two solutions were then pooled and added 5 volumes of -20 ºC Methanol: Ace- tone solution to remove SDS and stain as well as precipitation of the protein. After 24 hours acetone solution were discarded and pellet were air dried on ice. To renaturation of protein with Guanidine HCl, the pellet was suspended in the minimum amount of buffer A, containing 100 mM KCl, 1mM DTT, 12.5 mM MgCl2, 6 M Guanidine, 10% (v/v) Glycerol, 25 mM HEPES-KOH pH 7.6 and 0.1 mM EDTA. After 20 minutes incu- bation at room temperature, several volumes of buffer B, composed of 1mM DTT, 0.1 mM KCl, 0.01% (v/v) IGEPAL CA-630, 12.5 mM MgCl2, 10% (v/v) Glycerol, 25 mM HEPES-KOH pH 7.6 and 0.1 mM EDTA, were added and dialyzed at room temperature for 2–3 hours against buffer B. Protein concentration was measured by Warburg method. The protein solution was sterilized with a 0.45 μm filter (Schleicher and schuell, Germany) and stored at -20 ºC (Hager and Burgess 1980, Hunkapiller et al. 1983, Kadonaga 2007). Immunization Cattle were divided into two groups (treatment and control). The treatment group were intradermally injected with a mixture of 100 μg eluted protein and equivalent amount of Freund's adjuvant (Biogen) every two weeks for a total three immunizations (first injection with complete Freund's adju- vant and two another with incomplete adju- vant). In a similar procedure the control group were injected with sterile PBS (pH= 7.2) and equivalent amount of complete and incomplete adjuvant as followed above. Tick challenge Four weeks after the last injection, each cattle were infested with about 500 Rh. (B.) annulatus tick larvae as described previously (Nikpay et al. 2012) on their right flank. Tick parameters Infested animals were checked daily and recovered ticks were collected, counted, weighed and isolated. Engorged ticks were collected twice a day, at 8:00 am and 4:00 pm. Each five engorged ticks were placed in a tube and were maintained in incubator with a constant condition as described earlier. The degree of protection was determined by measuring the usual parameters in ticks fed on immunized animals and comparing the results with those fed on control animals. J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 285 http://jad.tums.ac.ir Published Online: January 06, 2016 These parameters were including: mean of recovered tick counts, attachment rate, en- gorgement weight, length of feeding period, feeding index (engorgement weight(mg)÷ feeding length (Day)), preoviposition period, egg mass weight, incubation period, hatch- ability rate and fertility index (egg mass weight(mg)÷engorgement weight (mg)). Statistical analysis The effect of treatment on level of tick fecundity and tick feeding parameters in two groups were analyzed by statistical methods. T-test and CHI square test was used to as- sess significant differences between immun- ized group means for each of variables (P< 0.05). All analyses were carried out using the SPSS program (version 16.0). Humoral response The levels of IgG antibodies produced against protein fractions of tick salivary gland extract injected to immunized cattle were monitored by ELISA according to the protocol described by Harlow (Harlow and Lane, 1988). Briefly, ELISA plates (Greiner) were coated with 5 µ g per well of antigen in 20 mM carbonate buffer (pH= 9.6) by incu- bation overnight at 4 ºC. They were washed three times and incubated for 1 h at 37 ºC with 1% bovine serum albumin (HAB-tech) in PBS. Then, test sera diluted 1:200 in PBS, were incubated for 1 h at 37 ºC. After this time, the plates were washed three times and 100 µ l of 1:2000 sheep anti –bovine IgG conjugated with peroxidase were added to the individual wells. After incubation at 37 ºC for 1 h the plates were washed. The color developed with 2, 2`, Azino-bis 3-ethyl Benz- Thiazoline-6-Sulfonic acid (ABTS) as substrate. The optical density (OD) was de- termined at 405 nm (AWARENESS- STAT FAX 2100). Sera from immunized and con- trol cattle were collected from week 0 (be- fore the first injection) until week 18 and results were compared in two groups. Results Determination of protein pattern by SDS- PAGE Coomassie blue staining of polyacryla- mide gel visualized a broad protein profile in Rh. (B.) annulatus tick salivary gland extract, comprised of 32 fractions with molecular weight of >200, 160, 107, 103, 92, 82, 80, 78, 72, 66, 63, 56, 55, 50, 46, 40, 38, 37, 36, 33, 30, 29, 27, 25, 22, 19, 18, 17, 16, 15 kDa and two bands lesser than 15 kDa (Fig. 1). Analysis of salivary gland antigens using bovine serum Positive serum from cattle infested with about 10000 Rh. (B.) annulatus tick larvae, recognized approximately 22 proteins from female tick salivary gland extract including two bands more than 170 kDa, 160, 103, 92, 78, 72, 66, 63, 56, 55, 50, 40, 38, 36, 33, 29, 27, 26, 25, 19 kDa and a band less than 17 kDa . Sera from uninfested animals did not react with any of these antigens by this assay (Fig. 2). From these immunogenic fractions, 14 bands, which were more dominant than oth- ers were (92, 78, 72, 66, 56, 55, 50, 40, 38, 36, 33, 27, 25 and 19 kDa), were dissected from polyacrylamide gel and used for im- munization procedure. Effects of immunization against tick chal- lenge Tick parameters We used two groups of parameters to as- sess the effects of immunization on ticks feeding parameters and fecundity parameters. Feeding parameters Effects of immunization on feeding pa- rameters are summarized in Table 1. Among immunized animals, the number, weight and feeding index of engorged female ticks were significantly reduced compared to the con- trol group. The length of feeding period in J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 286 http://jad.tums.ac.ir Published Online: January 06, 2016 immunized group was longer than control group (P< 0.05). Fecundity parameters Female ticks recovered from immunized cattle passed longer period to lay egg after engorgement in comparison with ticks har- vested from control cattle. The eggs were laid by ticks engorged on immunized cattle had longer incubation period than eggs pro- duced by ticks fed on control cattle. Im- munization with eluted antigens significantly reduced egg mass weight, hatchability rate of eggs and fertility index (P< 0.05) (Table 1). Humoral responses Cattle immunized with eluted fractions of salivary gland extract developed antibod- ies to these antigens. Humoral immune re- sponses to immunization with tick salivary glands eluted proteins measured by ELISA. Results showed a steady increase in antibody production level of treatment group from the first week after immunization until the last injection. After fourth week, it reached a plateau during next four weeks. When a tick challenge was performed at week eighth, it rose sharply and peaked at week 12 and then this trend declined and gradually stabilized at week 18. In control group, there was a pe- riod of stability during the first eight weeks. After tick infestation, it surged dramatically and reached a peak at week 12. Then, it fell slightly to a constant level. Although both groups have had a significant increase in their antibody production level after 18 weeks, this level was boosted following tick infestation in treatment group (Fig. 3). Table 1. Different parameters measured in female engorged ticks recovered from vaccinated and control groups and their change percentage Variable Group Mean Percent of changes Number of collected ticks Vaccinated 161 -63.1 1 Control 436 Attachment rate Vaccinated 32.2 -55 Control 87.2 Engorgement weight (g) Vaccinated 0.206 -30.2 Control 0.295 Length of feeding period (Day) Vaccinated 25.250 8.6 Control 23.511 Preoviposition period (Day) Vaccinated 2.906 45 Control 2.011 Egg mass weight (g) Vaccinated 0.403 -40 Control 0.665 Incubation period (Day) Vaccinated 25.406 31.34 Control 19.345 Feeding Index Vaccinated 0.008 -36.4 Control 0.013 Fertility Index Vaccinated 1.95 -13.3 Control 2.25 Hatchability rate Vaccinated 747 -78.7 Control 3504 1 Negative values indicates reduction in that value J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 287 http://jad.tums.ac.ir Published Online: January 06, 2016 Fig. 1. SDS-PAGE analysis of Rhipicephalus (Boophilus) annulatus salivary gland extract. Sample: SDS-PAGE gel stained with coomassie blue. Marker: protein ladder. MW: molecular weight Fig. 2. Western blot analysis of Rhipicephalus (Boophilus) annulatus salivary gland extract. Neg sera: serum of uninfested cattle. Pos sera: serum of infested animals with tick larvae. Prestain: prestained protein ladder. MW: molecular weight Fig. 3. Changes in antibody production to selected antigens in vaccinated animals in comparison with control ani- mals during 18 weeks Indicates when tick challenge was carried out. Vac: vaccinated animals. Control: control group J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 288 http://jad.tums.ac.ir Published Online: January 06, 2016 Discussion It is generally accepted that tick saliva plays an important role in pathogen trans- mission, suppression of host immune reac- tions, and successful engorgement. There- fore, biochemical and immunological char- acterization of tick saliva components intro- duced to the mammalian host during the feeding procedure is essential (Willadsen 1980, Brossard and Fivaz 1982, Wikel 1996, 1999a, b, 2006, Willadsen and Jongejan 1999). Some mammalian hosts acquired re- sistance to tick bites after repeated experi- mental infestation with ticks (Brossard and Fivaz 1982, Dipeolu et al. 1992, Nikpay et al. 2008, Nikpay et al. 2012). According to these researches, although feeding behavior of ticks was affected after several experi- mental tick infestations, some components of tick saliva acting as host immune re- sponse modulator, suppressed host immune system. Therefore, this method could not reject ticks from host properly (Dipeolu et al. 1992, Cobon et al. 1996, Wikel 1999a, b, Willadsen and Jongejan 1999). On the other hand, some researchers used various tissue extracts of ticks as a source of antigens for immunization of hosts. Since the salivary gland extracts contain the natu- ral antigens injected by the arthropod during infestation, it appears to be an obvious choice to induce immunity against blood-sucking arthropods. It has been shown Immunization with salivary gland extracts of Rh. (B.) annulatus tick resulted in a clear reduction in the severity of clinical babesiosis. In addition, fewer Babesia bigemina positive ticks were collected from these cattle (Jittapalapong et al. 2004a, Jittapalapong et al. 2004b). It is obvious that the importance of in- hibiting tick feeding to prevent direct damage and pathogen transmission and reliability of using natural antigens to avoid repeated tick infestation justifies further investigations on natural salivary gland antigens of ticks. Our results demonstrated that immun- ization of calves with eluted fractions of salivary gland extracts of Rh. (B.) annulatus ticks induced remarkable immunity ex- pressed as an inhibition of feeding and fer- tility of the ticks with less direct trauma and damage and without the risk of disease transmission. As mentioned in results, im- munization of cattle resulted in reduction in mean tick counts, attachment, engorgement weights, feeding index, egg mass weight, hatchability and fertility index (respectively 63.1%, 62.6%, 30.2%, 36.4%, 40%, 78.7% and 13.3%) and increased duration of feed- ing, preoviposition and incubation period of eggs (respectively 8.6%, 45 and 31.34%). Clearly, the host immunity affects the physi- ology of the tick in some permanent manner persisting even after the parasite detached. The shortening of the feeding time by the vaccine suggests that although the degree of immunity produced in this assay was rela- tively acceptable, the stronger immunization can probably inhibit feeding totally, which is highly desirable because it will prevent the transmission of tick- borne pathogens during feeding. The humoral immune responses, meas- ured by ELISA, showed cattle injected with tick salivary gland eluted proteins have ex- pressed a constant increase in antibody pro- duction level from the first week of injection which were boosted after tick challenge. We had demonstrated tick infestation elevates the antibody level in infested cattle (Nikpay et al. 2008). The results reported in this study con- firmed that immunization against Rh. (B.) annulatus could be achieved under experi- mental conditions. Therefore, the immuno- gen fractions of the salivary gland extract would be an appropriate target to design an effective vaccine against blood-sucking par- asites especially ticks. In addition, our pre- J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 289 http://jad.tums.ac.ir Published Online: January 06, 2016 vious studies indicated there are protein bands with the same molecular weight in dif- ferent tissues of Rh. (B.) annulatus tick re- sulted in similar changes in antibody pro- duction level in cattle (Nikpay et al. 2008, Nikpay et al. 2012). Comparison of the re- sults of SDS- PAGE and western blot analy- sis in this study and some previous studies reveals that there are several protein frac- tions having similar molecular weight and similar immunogenicity in different species of Ixodid ticks studied (Nabian et al. 2005, Razmi et al. 2005, Manzano-Román et al. 2007, Norouzi et al. 2007, Nikpay et al. 2008, Nikpay et al. 2012). Interestingly, some of these fractions were reported from the different tissues of ticks. Thus finding common crucial antigens in various tissues of different species of ticks would be our final goal in other studies. Conclusion Results of this investigation indicates that immunizing of cattle with these antigens could induce a protective immune response against Rh. (B.) annulatus tick infestation that would be expected to provide a safe non-chemical means of tick control. We would like to follow this study by charac- terization of each immunogenic fraction and finding common crucial antigens in other tick species to design a feasible and reliable vaccine against tick infestation Acknowledgements Authors wish to acknowledge the finan- cial support of the Research Council of Teh- ran University and Veterinary Medicine Fac- ulty of Tehran University. The authors declare that they have no Conflict of Inter- ests. References Brossard M, Fivaz V (1982) Ixodes ricinus L.: mast cells, basophils and eosinophils in the sequence of cellular events in the skin of the infested or reinfested rabbits. Parasitol. 85: 583–592. Brown SJ, Shapiro SZ, Askenase PW (1984) Characterization of tick antigens in- ducing host immune resistance. I. Immunization of guinea pigs with Amblyomma americanum-derived sali- vary gland extracts and identification of an important salivary gland pro- tein antigen with guinea pig anti-tick antibodies. J Immunol. 133: 3319– 3325. Center for Cocoa biotechnology research and development (2007) Protein detection: Gel Electrophoresis, Isolation and renaturation of proteins from SDS- PAGE gels, The Laboratory of Jim Kadonaga at the University of Cali- fornia, San Diego. Available at: http://www.koko.gov.my/CocoaBioTec h/Protein%20Detection7.html#title. Cobon GS, Moore JT, Johnston LAY, Willadsen P, Kemp DH, Sriskantha A, Riding GA, Rand KN (1996) DNA encoding a cell membrane gly- coprotein of a tick gut. US Patent and Trademark Office Appl. No. 325 071 435/240.2. Dipeolu OO, Mongi AO, Essuman S, Amoo AO, Ndungu JN (1992) Studies on naturally acquired immunity to African ticks. II. Observations on cattle ex- posed to Rhipicephalus appendiculatus under varying periods of repeated infestations. Vet Parasitol. 41: 293– 320. Estrada-peña A, Bouattour A, Camicas JL, Walker AR (2004) Rhipicephalus (Boophilus) annulatus, In: Ticks of domestic animals in the Mediterra- J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 290 http://jad.tums.ac.ir Published Online: January 06, 2016 nean region: a guide to identification of species. 1 Ed. Univ Zaragoza, Spain. FAO (2004) Acaricide resistance: diagnosis, management and prevention, in: Guidelines Resistance Management and Integrated Parasite Control in Ruminants, Animal Production and Health Division, Agriculture Depart- ment, Food and Agriculture Organ- ization of the United Nations, Rome, Italy, pp. 25–77. Hager DA, Burgess RR (1980) Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Esche- richia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem. 109: 76–86. Harlow E, Lane D (1988) Antibodies: A laboratory Manual. Cold Spring Har- bor Laboratory, New York. Hudson L, Hay FC (1984) Practical Immu- nology. 3rd Ed. Blackwell Scientific Publication, Oxford, UK. Hunkapiller MW, Lujan E, Ostrander F, Hood LE (1983) Isolation of mi- crogram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 91: 227–236. Jittapalapong S, Jansawan W, Barriga OO, Stich RW (2004a) Reduced inci- dence of Babesia bigemina infection in cattle immunized against the cattle tick, Boophilus microplus. Ann N Y Acad Sci. 1026: 312–318. Jittapalapong S, Jansawan W, Gingkaew A, Barriga OO, Stich RW (2004b) Pro- tection of dairy cows immunized with tick tissues against natural Boophilus microplus infestation in Thailand. Ann N Y Acad Sci. 1026: 289–294. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680–685. Manzano-Román R, García-Varas S, Encinas- Grandes A, Pérez-Sánchez R (2007) Purification and characterization of a 45-kDa concealed antigen from the midgut membranes of Ornithodoros erraticus that induces lethal anti-tick immune responses in pigs. Vet Parasitol. 145: 314–325. Mattewson MD, Wilson RG, Hamment CA (1975) The development of resistance to certain organophosphorus and carbamate Ixodicides by the blue tick, Boophilus decoloratus, in Rho- desia. Bull Ent Res. 66: 553–560. Murrell A, Barker SC (2003) Synonymy of Boophilus Curtice, 1891 with Rhipicephalus Koch, 1844 (Acari: Ixodidae). Systematic Parasitol. 56: 169–172. Nabian S, Rahbari S, Haddadzadeh H, Alidadi N (2005) A study on the effects of different temperatures on protein changes in the salivary glands of Hyalomma anatolicum anatolicum and Rhipicephalus (Boophilus) annulatus. Iranian J Vet Res Univ Shiraz. 6: 35–39. Nari A, Hansen JW (1999) Resistance of ecto- and endo-parasites: current and future solutions. 67th General Ses- sion. International Committee, 17–21 May, OIE. Paris, pp. 17–21. Nikpay A, Nabian S, Rahbari S, Shayan P, Alidadi N (2008) Detection of humoral immune response of calves to Boophilus Annulatus by ELISA. Irann J Parasitol. 3: 26–30. Nikpay A, Nabian S, Taheri M (2012) Anal- ysis of immunogenic relevant pro- teins in Rhipicephalus (Boophilus) annulatus tick. J Arthropod-Borne Dis. 6: 36–44. Norouzi F, Hashemitabar GR, Razmi GR (2007) Detection of midgut antigens J Arthropod-Borne Dis, September 2016, 10(3): 282–291 A Nikpay and S Nabian: Immunization of Cattle … 291 http://jad.tums.ac.ir Published Online: January 06, 2016 of Hyalomma anatolicum anatolicum tick using SDS-PAGE and Western blot. Iran J Vet Res. 8: 166–169. Rajput ZI, Hu SH, Chen WJ, Arijo AG, Xiao CW (2006) Importance of ticks and their chemical and immunological control in livestock. J Zhejiang Univ Science B. 7: 912–921. Razmi GR, Tabar GRH, Naghibi A (2005) Immunization of rabbits against Hyalomma anatolicum anatolicum tick using antigen derived from whole tick and gut extract. Iran J Vet Res. 6: 23–26. Tietz NW, Andresen BD (1986) Textbook of Clinical Chemistry. WB, Saunders, Philadelphia. Walker A (1994) The arthropods of humans and domestic animals: a guide to preliminary identification. Chapman and Hall, London, New York. Wang H, Nuttall PA (1994) Comparison of the proteins in salivary glands, saliva and haemolymph of Rhipicephalus appendiculatus female ticks during feeding. Parasitol. 109(4): 517–523. Wikel SK (1996) Host immunity to ticks. Annu Rev Entomol. 41: 1–22. Wikel SK (1999a) Modulation of the host immune system by ectoparasitic arthropods: blood-feeding and tissue- dwelling arthropods manipulate host defenses to their advantages. Bio Science. 49: 311–320. Wikel SK (1999b) Tick modulation of host immunity: an important factor in path- ogen transmission. Int J Parasitol. 29: 851–859. Willadsen P (1980) Immunity to ticks. Ad Parasitol. 18: 293–311. Willadsen P (2006) Tick control: thoughts on a research agenda. Vet Parasitol. 138: 161–168. Willadsen P, Jongejan F (1999) Immunology of the tick-host interaction and the control of ticks and tick-borne diseases. Parasitol. 15: 258–262.