J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 136 Original Article Effect of Iranian Honey bee (Apis mellifera) Venom on Blood Glucose and In- sulin in Diabetic Rats Seyyedeh Mahbubeh Mousavi 1, Sohrab Imani 2, Saeid Haghighi 3, Seyyedeh Elaheh Mousavi 4, Akbar Karimi 5 1Department of Entomology, Islamic Azad University, Tehran Science and Research Branch, Iran 2Department of Toxicology, Medical Sciences University, Semnan, Iran 3Department of Pharmaceutical, Microbiology, Pasture Institute of Iran, Tehran, Iran 4Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran 5Departments of Developmental Biology Sciences, Islamic Azad University, Tehran Science and Research Branch, Iran (Received 31 Oct 2010; accepted 3 Sep 2011) Abstract Background: Diabetes is an important disease. This disease is a metabolic disorder characterized by hyperglycemia resulting from perturbation in insulin secretion, insulin action or both. Honey bee venom contains a wide range of polypeptide agents. The principle components of bee venom are mellitin and phospholipase A2. These components increase insulin secretion from the β-cells of pancreas. This study was conducted to show the hypoglycemic effect of honey bee venom on alloxan induced diabetic male rats. Methods: Eighteen adult male rats weighting 200±20 g were placed into 3 randomly groups: control, alloxan mono- hydrate-induced diabetic rat and treated group that received honey bee venom daily before their nutrition for four months. Forty eight hours after the last injection, blood was collected from their heart, serum was dissented and blood glucose, insulin, triglyceride and total cholesterol were determined. Results: Glucose serum, triglyceride and total cholesterol level in treated group in comparison with diabetic group was significantly decreased (P< 0.01). On the other hand, using bee venom causes increase in insulin serum in com- parison with diabetic group (P< 0.05). Conclusion: Honeybee venom (apitoxin) can be used as therapeutic option to lower blood glucose and lipids in dia- betic rats. Keywords: Alloxan, Glucose, Honeybee Venom, Rat Introduction Diabetes type 1 is mainly results from auto- immune beta cell destruction, while viral in- fections and chemical agents seem to be the triggers of the disease. The well-documented effect of insulin is to mediate carbohydrates, proteins and lipids storage. Therefore diabetes is considered as a defect of lipids, proteins and carbohydrates metabolism in which all the body systems and organs are affected (Williams and Pickup 2000). The effect of insulin deficiency mainly results in elevation of cholesterol, phos- pholipids and free fatty acid (Williams and Pickup 2000). Natural toxins have been tra- ditionally used to heal diseases and honeybee venom (apitoxin) is of a great importance in this regard. The venom is composed of varieties of pep- tides (mellitin, apamin, secapin, tertiapin, ado lapin, and MCD peptid), enzymes (phospho- lipase A2, hyaluronidase, acid phosphomo- noesterase, lysophospholipase), active amines (histamine, dopamine, norepinephrine, sero- tonin) and many other substances (Son et al. 2007). Bee venom and Bee sting had signifi- *Corresponding author: Dr Sohrab Imani, E-mail: imanisohrab@yahoo.com J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 137 cant effect on quality of life (Wesselius et al. 2005). Mellitin and phos-pholipase A2 are the most important ingredients and play a great role in irritation and allergic responses leading to anti-inflammatory and analgesic effects (Mazzanti et al. 2007). Mellitin as the most important ingredient in bee venom is a strong phospholipase A2 activator which is composed of 26 amino acids and makes up 50% of the dried venom. Those bee venom ingredients that contain a high molecular weight, like hyaluronidase and phospho- lipase A2, cause the immune reactions (Bomalaski et al. 1989, Zalat et al. 1999). Apamin, mellitin and phospholipase A2 con- tained in bee venom, are strong immuno- regulators but their effects on diabetes have not been investigated (Habermann 1972, Gauldie et al. 1976). BV apamin is known as a neurotoxin from central nervus system hyperexcitability through inhibition of the axonal potassium channels (Son et al. 2007, Pedarzani et al. 2008). Melittin,the major constituent of bee venom, has an anti-inflammatory effect through its functionality on the anterior pitu- itary gland, which in turns may result in stimulation of cortisol production from the adrenal gland (Son et al. 2007). Part of anti- inflammatory effect of melittin may attribute to its interaction with cell surface moieties which seems to render the cytotoxic effects of melittin on cancer cells (Mirshafiey 2007, Son et al. 2007). With regard to the side effects and high prices of blood sugar lowering chemicals, achieving new therapeutic agents with low side effects seems to be necessary. Nowadays diabetic's population is growing rapidly while encountering various life-threatening disease conditions that require researchers to evaluate related therapeutic, alleviative and preventive treatments. The main purpose of the study was to evaluate the effects of Iranian honey- bee venom (apitoxin) on blood levels of glu- cose and insulin in alloxan induced diabetic rat. Materials and Methods Honeybee venom preparation procedure Bee venom samples were collected from beehives using an electric shocker, on February 2010 in Khuzestan, Iran. The electric shocker is composed of two components: one component as shocker and the other to collect the venom and concomitant material. The collecting unit is wooden and composed of a network of wires with small gaps between them. A glass plane is inserted under the network. The shocker was supplied by a transformer to produce a light electric shock. The shocker is designed to pro- duce a light electric shock once every few se- conds. The collector panel was first located on bottom of the beehives and then on the top, to collect the desired amount of the bee venom. When the shocker was turned on, the honey- bees stroked the wires and received a light elec- tric shock and were stimulated them to sting and discharged their bee venom. Alarm phero- mone produced by the exited bees to crowd and discharge toxin. The shocker was turned off after 25 minutes and the collecting panel was removed from the beehive, and the dried bee venom material scratched and transferred to a proper container. To evaluate the toxin produc- tion efficiency after collecting the samples, the crystallized bee venom material weighed using a sensitive scale. Animals Healthy adult male Lewwis rats weighting 200±20 g were maintained in Darou Pakhsh Pharmaceutical Mfg Co, Tehran (Iran). All animals left to acclimatize for one week be- fore the experiment. The animal room was maintained under a constant 12-h light: 12-h dark cycle and temperature of 23±3 °C and relative humidity of 70±10% throughout the experimental period. The rats were given free access to standard pellets and water. For bees to be adapted to the new environment J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 138 condition, all the experiments were carried out 10 days after their first residence. The research was approved by Ethical Committee of the university. Experimental design Eighteen rats were placed randomly into three groups (n= 6): Control group: normal saline 0/9% inject- ed intraperitoneally. Diabetic group: this group became dia- betic by injection Alloxan monohydrate at 150 mg/kg intraperitoneally. Bee venom-treated group: at first received Alloxan monohydrate to induce diabetes and Iranian bee venom (apitoxin) at 0/5 mg/kg (the best dose chosen after pretest) after dia- betes confirmation intraperitoneally at fasting condition every day for four consecutive weeks (Kim et al. 1999, Dong et al. 2007). Blood sugar test was performed in all groups. Blood glucose level was measured using Acua Check (Germany). To measure the blood glucose level, a small incision was made on the animals’ tail using a lancet and a drop of fresh blood was extracted and used for glucometery. These samples were collected in fasting condition and expressed in mg/dl. Alloxan monohydrate (Sigma-Aldrich Ger- many) was used to induce diabetes in rats. The drug was administrated intraperitoneally at the rate of 150 mg/kg (Viana et al. 2004, Antia et al. 2005). The drug administration leads to pancreas β -cells apoptosis and necrosis. The method is preferred to induce diabetes in many other animals (Soto et al. 2001). Following the administration a condition of hyperglycemia like diabetes type 1 appeared in rats (Byung- Hyun and Jin-Woo 2001). Seventy two hours after the administration, blood glucose level was measured using Acua Check to deter- mine diabetic condition. In this study, blood glucose level elevation over 280 mg/dl is sup- posed to reveal diabetic condition (Zhang and Tan 2003). Usual signs of diabetes including polydipsia, polyuria and weight loss were observed 6–7 days following the administra- tion (Nuraliev et al. 1992, El- demerdash et al. 2005). To measure glucose level, fasting blood samples were collected 2 weeks following bee venom administration using "Stone" method. In this method, venous blood was collected from infraorbital sinus using a hematocrit tube. The animal is kept between forefinger and thumb while the tube is inserted to the orbital foramen with a rotational movement. The capillaries are usually very sensitive and fragile here and burst following a light pres- sure. When a few almost large drops of blood were collected, the hematocrit tube is removed. The method is suitable for repet- itive blood sampling especially for primary analysis following 2 weeks of administra- tion. The blood glucose level was deter- mined using enzymatic kits. Biochemical analysis The animals were anesthetized with ether after 16 h of fasting to collect blood for anal- ysis. Forty eight hours after the last in- jection, blood samples were collected from the heart and centrifuged (3000 rpm for 15 min at 4 °C) for separating the serum. The serum was then frozen at -70 °C for the bio- chemical analysis. Then the amount of blood glucose, insulin, cholesterol and triglyceride in blood serum weredetermined.Serum glu- cose level was measured by kinetic (enzy- matic) and colorimetric methods using Glu- cose Estimation Kit (Pars Azmoon, Iran). Theserum insulin levels were assayed with an ELISA, IRMA (Biosource Europe SA), serum glucose, triglyceride and total cholesterol lev- els were determinedusingcommercial kits and enzymatic assays. Statistical analysis The values were expressed as mean ±S.E.M Statistical analyses were performed by one J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 139 way analysis of variance (ANOVA) fol- lowed by Tukey multiple comparison test. P< 0.05 were considered as significant. Results Bee venom preparation results: the bee venom samples were collected and panel scratched using a sterile blade and kept in clean dark vials. Average amount of venom collected from three beehives (each con- taining estimated 10000 bees) was 147.7 mg. The average for each beehive was calculated 49.56 mg. Alloxan administration result showed a significant increase in blood glu- cose level in (diabetic) group compared with control group after 2 and 4 weeks (P< 0.05). In addition a significant increase was ob- served in blood glucose level in diabetic group after 4 weeks compared with 2-week period (P< 0.05). There was a significant de- cline in blood glucose level in bee venom- treated group compared with diabetic group (P< 0.05). Also there was a significant de- cline in blood glucose level in diabetic treated group after 4 weeks compared with 2-week pe- riod (P< 0.05) (Fig. 1). The effect of bee venom administration on serum insulin level is illustrated in Figure 2. Our results showed that serum insulin lev- el significantly decreased in diabetic group compared with controls (P< 0.05). Also there was a significant increase in bee ven- om-treated group compared with controls (P< 0.05) (Fig. 2). The effect of bee venom administration on serum triglyceride (TG) content is illustrated in Figure 3. There was a significant increase in serum TG content in diabetic group com- pared with controls (P< 0.05). A significant decline was observed in bee venom-treated group compared with diabetic animals (P< 0.05). But no significant difference was ob- served in serum TG content in bee venom- treated group compared with control (Fig. 3). A significant increase was observed in se- rum total cholesterol in diabetic group com- pared with controls (P< 0.05). There was a significant decline in bee venom-treated group compared with diabetic animals (P< 0.05). But no significant difference was ob- served in cholesterol content in bee venom- treated group compared with control group (P= 0.552) (Fig. 4). Fig. 1. Changes of blood glucose level after injection of bee venom in diabetic rats J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 140 Fig. 2. Serum insulin levels after injection of bee venom in diabetic rats. Difference between control and treated group are significant with *P< 0.05. Fig. 3. Triglyceride levels after injection of bee ven- om in diabetic rats. Difference between control and treated group are significant with *P< 0.05. Fig. 4. Cholesterol levels after injection of bee venom in diabetic rats. Difference between control and treated group are significant with *P< 0.05. Discussion In our study, blood glucose level increased following alloxan monohydrate administration which led to pancreas B-cells destruction (Byung-Hyun and Jin-Woo 2001). Blood glu- cose level decreased following bee venom treatment. This may be contributed to substanc- es like mellitin and phospholipase A2 contained in the venom. They may play a role in dimini- shing inflammation of Islets of Langerhans and thus elevating blood insulin level. With regard to the fact that insulin regulates blood glucose level, bee venom could decrease glucose con- tent via increasing insulin secretion (Morgan and Montague 1984, Fujimoto and Metz 1987, Kim et al. 1999, Simonson et al. 2000). Follow- ing alloxan administration in diabetic J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 141 rats, blood glucose level and triglyceride (TG) content were elevated, which indicate insulin role in regulating lipid metabolism (Zhang and Tan 2003). Insulin activates the enzyme lipo- protein lipase and hydrolysis triglycerides (Frayn 1993). According to the obtained results, bee venom decreased blood TG content. One could explain the observed decline as follows: bee venom improves glycemic control and de- creases blood glucose level. Also glucose con- sumption is increased instead of lipids. Acetyl coA derived from pyrovic acid enters Krebs cycle which finally leads to glucose metabo- lism,however Acetyl coA can enter TG synthe- sis pathway in usual condition (Zhang and Tan 2003). Blood cholesterol level increased fol- lowing Alloxan administration. (Yadav et al. 2004). A decline was observed in cholesterol content of bee venom -treated group (Kim et al. 1999). Probably cholesterol lowering effect is largely due to inhibition of its absorption in small intestine and promoting its hepatic re- lease. The liver plays a critical role in dis- charging cholesterol via bile secretion (Reinner et al. 1989). Alloxan administration led to destruction of Islets of Langerhans and diminished insu- lin secretion in diabetic rats. Treating the rats with honeybee venom (apitoxin) in- creased insulin secretion up to control levels. According to the published reports, mellitin polypeptide and phospholipase A2, which are two main component of the venom, promote insulin secretion. According to the literature, the observed effect is mediated by extracellu- lar calcium and calcium channels. When the- se channels are opened large amounts of cal- cium enters the β-cells and excite them to se- cret insulin (Morgan and Montague 1984, Fu- jimoto and Metz 1987, Kim et al. 1999, Si- monson et al. 2000). In a study on the effects of bee venom on Islets of Langerhans inflam- mation and onset of insulin dependent diabe- tes, Kim et al. (1999) showed that intensity of inflammation and onset of diabetes declined following bee venom treatment. They also found that insulin, TG and cholesterol levels decreased in diabetic rats compared with non- diabetic animals (Kim et al. 1999). According to the experiments of Morgan et al. (1984), mellitin polypeptide promotes insulin secre- tion from Islets of Langerhans in vitro. The obtained results suggest that mellitin as a valu- able candidate for further studies on β-cells plasma membrane role in regulating insulin secretion. The findings also indicate that mellitin can depolarize plasma membranes of β-cells and acts as a calcium transporter in the cell, which in turn promotes insulin granules secretion. The effect of mellitin on insulin se- cretion depends on extracellular calcium (Mor- gan and Montague 1984). Simonson et al. (2000) found that mellitin may promote insulin secretion via activating phospholipase A2 in Islets of Langerhans. Their results indicate that phospholipase A2 activation plays a role in compensating insulin resistance response in Is- lets of Langerhans (Simonson et al. 2000). Treatment with exogenous phospholipase A2 or mellitin promotes arachidonic acid and lysophospholipids production and insulin se- cretion. Produced arachidonic acid and lyso- phospholipids may corporate in two-step in- sulin production mechanism. Arachidonic acid produced by phospholipase A2 induction may act as a calcium transporter in to β -cells and promote insulin secretion (Fujimoto and Metz 1987). Conclusion In this study, our results indicate that hon- eybee venom (apitoxin) can be used as thera- peutic option to lower blood glucose and li- pids in diabetic rats, however further bio- chemical and pharmacological studies are necessary to provide more detailed under- standing of the issue. Acknowledgements This study was supported by the Darou J Arthropod-Borne Dis, 2012, 6(2): 136–143 SM Mousavi et al.: Effect of Iranian Honey … 142 Pakhsh pharmaceutical Mfg Co, Tehran (Iran). The authors declare that there is no conflict of interests. References Antia BS, Okokon JE, Okon PA (2005) Hy- poglycemic effect of aqueous leaf ex- tract of Persea Americana Mill on alloxan induced diabetic rats. 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