J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 163 Original Article Fauna and Larval Habitats of Mosquitoes (Diptera: Culicidae) of West Azerbaijan Province, Northwestern Iran Farahnaz Khoshdel-Nezamiha 1,2, Hassan Vatandoost 3 , Shahyad Azari-Hamidian 4, Mulood Mohammadi Bavani 3, Farrokh Dabiri 2, Rasool Entezar-Mahdi 5, *Ali Reza Chavshin 1,2 1Research Center of Social Determinants of Health, Urmia University of Medical Sciences, Urmia, Iran 2Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran 3Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 4School of Health, Guilan University of Medical Sciences, Rasht, Iran 5Director of Health Deputy of West Azerbaijan, Urmia University of Medical Sciences, Urmia, Iran (Received 23 June 2013; accepted 26 Oct 2013) Abstract Background: Several important diseases are transmitted by mosquitoes. Despite of the potential of the occurrence of some mosquito-borne diseases such as West Nile, dirofilariasis and malaria in the region, there is no recent study of mosquitoes in West Azerbaijan Province. The aim of this investigation was to study the fauna, composition and dis- tribution of mosquitoes and the characteristics of their larval habitats in this province. Methods: Larvae and adult collections were carried out from different habitats using the standard methods in twenty five localities of seven counties across West Azerbaijan Province. Results: Overall, 1569 mosquitoes including 1336 larvae and 233 adults were collected from 25 localities. The de- tails of geographical properties were recorded. Five genera along with 12 species were collected and identified in- cluding: Anopheles claviger, An. maculipennis s.l., An. superpictus, Culex pipiens, Cx. theileri, Cx. modestus, Cx. hortensis, Cx. mimeticus, Culiseta Longiareolata, Ochlerotatus caspius s.l., Oc. geniculatus and Uranotaenia unguiculata. This is the first record of Oc. geniculatus in the province. Conclusion: Due to the geographical location of the West Azerbaijan Province, it comprises different climatic con- dition which provides suitable environment for the establishment of various species of mosquitoes. The solidarity geographical, cultural and territorial exchanges complicate the situation of the province and its vectors as a threat for future and probable epidemics of mosquito-borne diseases. Keywords: Culicidae, Arboviruses, Larval habitat, Mosquito-borne diseases, Fauna Introduction The family Culicidae (Diptera) comprises at least 3531 species representing 111 genera divided into two subfamilies, Anophelinae and Culicinae (Harbach 2013). Several important diseases are transmitted by them, among which some of mosquito-borne viruses such as West Nile and Sindbis have been reported from Iran (Naficy and Saidi 1970, Saidi et al. 1976). Chinikar et al. (2010) reported a case of Den- gue fever in Tehran, imported from Malaysia Moreover the possibility of the outbreak of some other mosquito-borne arboviruses such as Japanese encephalitis and Rift Valley fe- ver in the WHO Eastern Mediterranean Re- gion, including Iran, should be considered (WHO 2004). Other mosquito-borne diseas- es such as dirofilariasis (Azari-Hamidian et al. 2009) and many other mosquito-borne dis- eases are endemic in some parts of Iran, in- cluding West Azerbaijan Province. *Corresponding author: Dr Ali Reza Chavshin, E-mail: chavshin@umsu.ac.ir http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 164 Several studies have been conducted to identify the fauna of mosquitoes across Iran (Zaim 1987, Vatandoost et al. 2004, Ghavami and Ladonni 2005, Vatandoost et al. 2006, Abai et al. 2007, Azari-Hamidian 2007b, Moosa-Kazemi et al. 2009, Azari-Hamidian et al. 2010, Azari- Hamidian 2011, Oshaghi et al. 2011, Hanafi-Bojd et al. 2012, Khoobdel et al. 2012, Saghafipour et al. 2012, Banafshi et al. 2013, Soleimani-Ahmadi et al. 2013). According to the most updated checklist of Iranian mosquitoes, 64 species representing seven genera occur in the country (Azari- Hamidian 2007a). In addition to morpholog- ical studies, molecular studies have been car- ried out in order to clarify some problematic situations (Sedaghat et al. 2003, Oshaghi et al. 2007, Naddaf et al. 2012). Oshaghi et al. (2008) recently identified Anopheles superpictus Grassi as a complex of three genotypes (X, Y, and Z). Naddaf et al. (2010) reported An. fluviatilis James species U from Fars Province and were in doubt about the occurrence of the species (form) V in Iran. Besides, Mehravaran et al. (2011) recorded the species U in southeastern Iran. Mosquito oviposition sites include different types which may differ in the point of the quantity and quality of water and environ- mental characteristics (Machault et al. 2009). Each mosquito species prefer certain breeding site for oviposition. The breeding habitat plays a crucial role for mosquito population dynam- ics. There is a strong association between the density and distribution of the mosquito lar- val stages and that of the adults. Then larval control programs can reduce the health prob- lems caused by mosquitoes (Floore 2006). The province of West Azerbaijan is located at the northwestern part of Iran and has a com- mon border line with other countries such as Turkey, Armenia, Azerbaijan and Iraq. The sol- idarity geographical, cultural and territorial ex- changes complicate the situation of the prov- ince. An example of such complexity is the collapse of the Soviet Union and the destruc- tion of health infrastructures in the newly in- dependent countries, leading to resurgence and outbreaks of malaria in the region and neigh- boring countries such as Iran (Greenwood et al. 2002, Lak et al. 2002, Vatandoost et al. 2003). Furthermore the presence of other mos- quito-borne diseases such as West Nile fever (Ahmadnejad et al. 2011) and dirofilariasis (Azari-Hamidian et al. 2007) in the province indicates the importance of investigation of mosquitoes in this region. According to the re- sults of the previous studies, 24 species of mos- quitoes in six genera have been reported from the province, although there is doubt about the presence of some species (Beklemishev and Gontaeva 1943, Zolotarev 1945, Kalandadze and Kaviladze 1947, Lotfi 1973, Saebi 1987, Sedaghat et al. 2003). There is no recent study of mosquitoes in West Azerbaijan Province, despite of the oc- currence of some mosquito-borne diseases such as West Nile, dirofilariasis, and malaria in the region. The aim of this investigation was to study the fauna, composition and distribution of mosquitoes in this province. Materials and Methods Study area West Azerbaijan Province is located in the northwest of Iran, bordering the coun- tries: Turkey, Iraq, Armenia, Azerbaijan, and the provinces of East Azerbaijan, Zanjan and Kurdistan. It covers an area of 39,487 km², or 43,660 km² including Lake Urmia. The 12 counties of this province have been located in different climate and geographical areas such as mountainous areas near the border with Iraq and Turkey, plains near the Aras and other rivers and the coast-line of the Urmia Lake (Fig. 1). The geographical details of collecting localities have been presented in Table 1. http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 165 Specimen and data collection In the present study, larvae collection was carried out from different habitats using the standard (350 ml dipper) dipping method (Silver 2008) in twenty five localities of seven counties across the province (Table 1). Sample collections were done during May– November 2012. The larvae were transferred to the laboratory and the microscope slides of the larvae were prepared. The third- and fourth-instar larvae were identified using the keys of Shahgudian (1960) and Azari-Hamidian and Harbach (2009). For collecting the adult mosquitoes, sev- eral methods such as hand catch, night land- ing catch on human, cow, total catch, and pit shelter collection were employed fortnight (Silver 2008). Collected samples were trans- ferred to the laboratory and identified using the standard keys (Azari-Hamidian and Harbach 2009). The abbreviations of the genus name have been used according to Reinert (Reinert 2009). Physical and biological characteristics of larval habitats Various physical and biological charac- teristics of the breeding places of larvae in- cluding habitat type (natural or artificial), wa- ter condition (standing, slow running, transi- ent or permanent water) vegetation (presence of vegetation and the kind of vegetation) and sunlight exposure (full or partial sunlight or shaded) were recorded visually or using prop- er equipments. Results Specimen collections were carried out on 35 occasions during May–November 2012. Overall, 1569 mosquitoes including 1336 lar- vae and 233 adults were collected from 25 localities. The details of geographical prop- erties (Latitude, Longitude and altitude) of localities are presented in Table 1. Five genera along with 12 species were collected and identified, including: An. claviger, An. maculipennis s.l., An. superpictus, Cx. pipiens, Cx. theileri, Cx. modestus, Cx. hortensis, Cx. mimeticus, Cs. longiareolata, Oc. caspius s.l., Oc. geniculatus and Ur. unguiculata (Table 2). This is the first record of Oc. geniculatus in West Azerbaijan Province. The species, Ur. unguiculata and Cx. mimeticus were collected only in larval stage and Oc. caspius s.l., and Oc. geniculatus on- ly as adults adjacent to trees and green spac- es using hand catch method. In larval stage, Cx. pipiens (25%), Cx. theileri (21%), Cx. hortensis (13.4%) and An. maculipennis com- plex (11.6%), were the most prevalent spe- cies respectively but in contrast in adult stage, An. maculipennis (41.6%), Cs. longiareolata (19.3%), An. claviger (18.45%), Cx. pipiens (3.86%) and Cx. hortensis (3.86%) were the most abundant species. However, Ur. unguiculata (0.07%) was scarce species (Table 2). Species such as Cx. pipiens, Cx. theileri and An. maculipennis have a wide distribution in the study areas but some species such as Oc. geniculatus, Oc. caspius and Ur. unguiculata have been collected from limited areas (Table 3). All of the different types of habitats were occupied by mosquito larvae. The breeding places with vegetation were more favorable for larvae. Most of Anopheles larvae were col- lected from permanent, still and clear water. Artificial habitats like artificial standing wa- ter (Nav-Jihan), slow running water (Ghezel- Kurd, Ghezel-Ajam) were the most common habitats for culicinae larvae but most of Anoph- eles larvae were collected from natural hab- itats like natural standing water (Bazargan) and transient water (Zakerloo). Likewise the members of the genus Culex were collected from wider range of habitats and this shows their adaptation ability to different conditions. The properties of larval habitats have been shown in Table 4. http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 166 Table 1. Geographical properties of collecting localities, West Azerbaijan Province, Iran, 2012 Location Latitude Longitude Altitude (m) Urmia Nazloo 1 37°39'24.39"N 44°59'0.39"E 1358 Nazloo2 37°39'0.69"N 44°59'5.50"E 1365 Sero 37°43'50.12"N 44°39'33.78"E 1572 Nav-Jehan 37°42'51.58"N 44°39'41.83"E 1605 Bavan-kanisi 37°49'28.46"N 44°44'3.31"E 2071 Marmisho 37°34'45.91"N 44°37'50.47"E 1749 Issar 37°33'25.95"N 45° 0'12.52"E 1466 Makoo Ghale-Joogh 39°16'46.06"N 44°28'19.15"E 1299 Baghche-joog 39°17'21.80"N 44°25'29.81"E 1411 Sangar 39°18'59.73"N 44°25'53.99"E 1348 Boljak 39°17'7.10"N 44°41'24.22"E 991 Ghezel- Kurd 39°15'27.66"N 44°40'0.29"E 1012 Ghezel- Ajam 39°15'51.23"N 44°39'12.11"E 1021 Bazargan Bazargan 1 39°24'31.87"N 44°26'11.77"E 1400 Yarim-Ghiye 39°26'45.90"N 44°26'4.27"E 1409 Poldasht Poldasht 39°36'2.58"N 44°51'43.20"E 795 Ghizil arkh 39°24'25.66"N 44°58'58.85"E 788 Zakerloo 39°21'55.62"N 45° 3'16.74"E 782 Sarisoo 39°20'26.52"N 44°53'58.04"E 858 Naghadeh Naghadeh 36°57'28.22"N 45°21'51.71"E 1313 Mahabad Mahabad1 36°48'53.18"N 45°44'3.86"E 1304 Kavelan 36°23'45.33"N 45°40'32.42"E 1538 Mahabad2 36°45'42.49"N 45°42'23.85"E 1371 Sardasht Sardasht Pol 36° 9'20.63"N 45°32'7.05"E 994 Kapran 36° 9'40.37"N 45°24'41.30"E 1562 Table 2. Relative abundance of adults and larvae of mosquitoes West Azerbaijan Province, Iran Species Adults Larvae n % n % An. claviger 43 18.45 99 7.4 An. maculipennis 97 41.6 155 11.6 An. superpictus 3 1.28 15 1.12 Cx. hortensis 9 3.86 180 13.4 Cx. mimeticus 0 0 7 0.52 Cx. modestus 3 1.28 47 3.5 Cx. pipiens 9 3.86 336 25 Cx. theileri 14 6 281 21 Cs. longiareolata 42 19.3 215 16 Oc. caspius 6 2.57 0 0 Oc. geniculatus 4 1.7 0 0 Ur .unguiculata 0 0 1 0.07 Total 233 100 1336 100 Fig. 1. West Azerbaijan Province and the location of the studied counties, 1) Bazargan, 2) Poldasht, 3) Makoo, 4) Urmia, 5) Naghadeh, 6) Mahabad and 7) Sardasht http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 167 Table 3. Composition and abundance of mosquitoes, West Azarbaijan Province, Iran, 2012 County Mosquito- life stage Species of Mosquito C x. pipien s C s. lon giareolata C x. th eileri C x. m odestu s C x. h orten sis A n .claviger A n .m acu lipen n is O c. gen icu latu s O c. caspiu s U r. an otaen ia A n . su perpictu s C x. m im eticu s Urmia Larvae 146 212 160 19 153 63 63 - - - - - Adult 5 34 3 - 1 26 65 - - - - - Makoo Larvae 80 3 20 20 - - 1 - - - - - Adult 2 - 3 3 - 1 6 4 6 - - - Bazargan Larvae - - 10 1 - - 21 - - - - - Adult 1 - 3 - 5 - 4 - - - - - Poldasht Larvae 55 - 86 3 - - 7 - - 1 - - Adult - - 3 - - - 3 - - - - - Naghadeh Larvae - - - - 26 40 - - - - - Adult - 7 - - 6 16 - - - - - Mahabad Larvae 55 - 5 4 27 - 8 - - - 5 7 Adult 1 4 2 - 3 1 - - - 3 - Sardasht Larvae - - - - - 10 15 - - - 10 - Adult - - - - - 9 3 - - - - - Total Larvae (%) 336(25%) 215(16%) 281(21%) 47(3.5%) 180(13.4%) 99(7.4%) 155(11.6%) - - 1(0.07) 15(1.12%) 7(0.52%) Adult (%) 9(3.86%) 45(19.3%) 14(6%) 3(1.28%) 9(3.86%) 43(18.45) 97(41.6%) 4(1.7%) 6(2.57%) - 3(1.28%) - Total (%) 345(22%) 260(17%) 295(19.8%) 50(3%) 189(12%) 142(9%) 252(16%) 4(0.25%) 6(0.38%) 1(0.19%) 18(1.1%) 7(0.46%) http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 168 Table 4. The characteristics of larval habitats of collecting localities, West Azerbaijan Province, Iran, 2012 Location Habitat situation Vegetation situation Sunlight situation Habitat kind Collected species (abundance percentage in the location) Urmia Nazloo 1 Slow running water out of water partial natural Cx. pipiens (25%), Cs. longiareolata (30%), Cx. theileri (20%), Cx. modestus (6%), Cx. hortensis (11%), An. claviger (8%) Nazloo2 transient water surface partial artificial Cx. modestus (76%), An. maculipennis (24%) Sero Slow running water beneath of water surface full natural Cx. theileri (55%), An. maculipennis (45%) Nav-Jehan Slow running water beneath of water surface partial artificial Cx. theileri (45%), An. maculipennis 25%), An. claviger (30%) Bavan-kanisi Standing water out of water partial artificial Cx. pipiens (100%) Marmisho permanent beneath of water surface shaded natural An. claviger (15%), Cx. hortensis (85%) Issar Transient beneath of water surface full artificial Cx. pipiens (20%), Cs. longiareolata (60%), Cx. theileri (15%), An. maculipennis (5%) Makoo Ghale-Joogh permanent out of water partial natural Cx. theileri (55%), Cs. longiareolata (45%) Baghche- joog Slow running water out of water shaded natural Cx. pipiens (40%), Cx. theileri (60%) Sangar Standing water beneath of water surface partial natural Cx. pipiens (70%), Cx. theileri (15%), Cx. modestus (15%) Boljak Standing water water surface shaded natural An. maculipennis (100%) Ghezel- Kurd Slow running water out of water partial artificial Cx. pipiens (65%), Cx. theileri (35%) Ghezel- Ajam Slow running water beneath of water surface shaded artificial Cx. theileri (80%), An. maculipennis (20%) Bazargan Bazargan 1 Standing water water surface full natural An.maculipennis (100%) Yarim-Ghiye Slowly running water beneath of water surface full artificial Cx. theileri (80%), Cx. modestus (5%), An. maculipennis (15%) Poldasht Poldasht permanent water surface partial natural Cx. pipiens (30 %), Cx. theileri (30 %), Cx. modestus (20 %), An. maculipennis (20 %) Ghizil arkh Standing water out of water full natural Cx. theileri (100%) Zakerloo Transient beneath of water surface partial natural An. maculipennis (95%), Uranotaenia (5%) Sarisoo Standing water water surface shaded natural Cx. pipiens (85%), An. maculipennis (15%) Naghadeh Naghadeh transient out of water full natural An. claviger (45%), An. maculipennis (55%) Mahabad Mahabad1 permanent water surface full natural Cx. pipiens(35%), Cx. theileri (10%), Cx.modestus (10%) Cx. hortensis (30%), An. maculipennis (15%) Kavelan Slowly running water beneath of water surface partial natural Cx. hortensis (15%), An. maculipennis (20%), An. superpictus (25%), Cx. mimeticus (40%) Mahabad2 Standing water water surface shaded natural Cx. pipiens (20%), Cx. theileri (25%), Cx. modestus (10%), An. maculipennis (25%), An. superpictus (20%) Sardasht Sardasht Pol Slowly running water beneath of water surface shaded natural An. maculipennis (65%), An. superpictus (35%) Kapran Slowly running water water surface shaded natural An. maculipennis (45%), An. superpictus (30%), An. claviger (25%) http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 169 Discussion This is the first specific and targeted in- vestigation to study mosquitoes in West Azerbaijan Province which showed the pres- ence of 12 species of mosquitoes in the study area. Sporadic studies had been con- ducted in parts of the north western part of Iran (Beklemishev and Gontaeva 1943, Zolotarev 1945, Kalandadze and Kaviladze 1947, Lotfi 1973, Danilov 1975, Saebi 1987, Azari-Hamidian et al. 2009, Kazemi et al. 2010, Banafshi et al. 2013). In addition to the 12 species collected and identified in this study, other 13 species such as An. algeriensis, An. plumbeus, Cx. deserticola, Cx. tritaeniorhynchus, Cs. alaskaensis, Cs. annulata, Oc. dorsalis (Kalandadze and Kaviladze 1947), An. hyrcanus Group (Saebi 1987), An. marteri (unpublished data from Institute of malariology, Tehran University of Medical Sciences), An. sacharovi (Kalandadze and Kaviladze 1947, Sedaghat et al. 2003), Cs. subochrea (Kalandadze and Kaviladze 1947, Zaim 1987), Aedes vexans (Kalandadze and Kaviladze 1947, Zaim 1987), Oc. flavescens (Kalandadze and Kaviladze 1947, Zaim 1987), were reported from this region. Because of the oldness of the report of some of these species and the probable effect of the climate change during the time, the presence of some of these species in the study area is questionable and uncertain. Seven genera and 15 species were identi- fied in East Azerbaijan Province (Abai et al. 2007) among which eight species (An. maculipennis, An. claviger, An. superpictus, Cx. theileri, Cx. pipiens, Cs. longiareolata, Oc. caspius and Ur. unguiculata) were common between the two neighboring provinces and six (Cx. theileri, Cx. pipiens, Cx. hortensis, Cx. mimeticus, Cs. longiareolata and Oc. caspius) are common between West Azerbaijan and Sanandaj County in Kurdistan Province which is in southern neighboring of West Azerbai- jan (Kazemi et al. 2010). The comparison of the results of this study with a recent comprehensive study carried out in Kurdistan Province (Banafshi et al. 2013) showed that nine species (An. maculipennis, An. claviger, An. superpictus, Cx. theileri, Cx. pipiens, Cx. hortensis, Cx. mimeticus, Cs. longiareolata and Oc. caspius) were common between Kurdistan and West Azerbaijan Prov- inces. Comparing the results of this study with a similar study carried out in Zanjan Province (Ghavami and Ladonni 2005), showed that six species (An. maculipennis, An. superpictus, Cx. pipiens, Cx. theileri, Cx. hortensis and Cs. longiareolata) are common between Zanjan and West Azerbaijan Provinces. In Turkey (Aldemir et al. 2010) and north- ern and central parts of Iran such as East- Azerbaijan, Ardebil and Kurdistan Prov- inces, Cx. theileri is the dominant and most abundant species, except for the low-lying areas bordering the Caspian Sea and urban areas (Azari-Hamidian et al. 2009). The re- sults of this study showed that in the case of larvae collection, Cx. pipiens (25%) and Cx. theileri (21%) were the most abundant spe- cies respectively and widely distributed in all parts of West Azerbaijan Province. The presence of these two species (Cx. pipiens, Cx. theileri) in other studies in Iran (Azari-Hamidian 2007b, Azari-Hamidian et al. 2009, Kazemi et al. 2010, Nikookar et al. 2010, Saghafipour et al. 2012) and other neighboring areas in neighboring countries such as eastern parts of Turkey and Iraq (Rueda et al. 2008) show a wide distribution of these species in the region. The species Oc. geniculatus is recorded for the first time in West Azerbaijan Prov- ince but this tree-hole mosquito has not been reported from neighboring areas of Iran and other neighboring countries. This species had been reported previously from northern parts of the country such as Ardabil, Guilan and http://jad.tums.ac.ir Published Online: April 09, 2014 J Arthropod-Borne Dis, December 2014, 8(2): 163–173 F Khoshdel-Nezamiha et al.: Fauna and Larval … 170 Mazandaran Provinces (Zaim 1987, Azari- Hamidian et al. 2009). The West Azerbaijan Province comprises different geographical areas with climati- cally different condition. These diverse cli- matic conditions can provide suitable environ- ment for the establishment of various species of mosquitoes and this is the explanation for the richness of the composition of mosquito species in this province. Some of identified species are principal vectors of several mosquito-borne pathogens such as West Nile virus, Rift valley virus. Six species of known species in this study (An. maculipennis, Cx. modestus, Cx. theileri, Cx. pipiens, Cs. longiareolata, Oc. caspius) are among the important vectors of West Nile fever. According to reports of the exist- ence of this disease in the study area (Saidi et al. 1976, Ahmadnejad et al. 2011) and sur- rounding countries (Zeller and Schuffenecker 2004), the existence of mentioned species should be considered and further investiga- tions are needed. Minding this fact that birds are involved in the cycle of transmission as amplifying hosts and existence of several ponds such as Urmia Lake, Mahabad pond, Aras basin and Sardasht nature which are the destination of migrant birds from other countries, emerging of this disease in the region is more conceivable. Picking these facts together should act like a wake for health system and more attention will turn to the mosquito-borne diseases. On the other hand, Cx. pipies and Cx. theileri, which have been identified in this study, reported as potential vectors of Rift Valley Fever (Zeller and Schuffenecker 2004). The presence of this disease in Mediterra- nean Region (Moutailler et al. 2008) indi- cates the need for more attention to this disease and its vectors. The results of this study also revealed the presence of the vectors of helminths such as Setaria labiatopapillosa and Dirofilaria immitis in the region. Anopheles maculippenis and Cx. theileri are well known vectors of the mentioned parasites in Ardebil Province (Azari-Hamidian et al. 2009). Conclusion The results of this study revealed the presence of different species of mosquitoes across the West Azerbaijan Province. Among the reported species, some of them are prob- able vectors of important mosquito-borne dis- eases. Further studies are needed regarding the epidemiology of mosquito-borne diseas- es and the role of mentioned species. Acknowledgements This article is a part of the results of the first author’s dissertation for fulfillment of MSc degree in Medical Entomology and Vector Control from Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran. This study was finan- cially supported by the Urmia University of Medical Sciences (Project no. 1202). The au- thors would like to thank the health staffs of the selected counties for their collaboration and positive appetite. The authors declare that there is no conflict of interest. References Abai MR, Azari-Hamidian S, Ladonni H, Hakimi M, Mashhadi-Esmail K, Sheikh- zadeh K, Kousha A, Vatandoost H (2007) Fauna and checklist of mosqui- toes (Diptera: Culicidae) of East Azer- baijan Province, northwestern Iran. Iran J Arthropod Borne Dis. 1(2): 27–33. 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