Microsoft Word - Dr Soltani RTL.doc Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 12 Original Article Use of Expanded Polystyrene (EPS) and Shredded Waste Polystyrene (SWAP) Beads for Control of Mosquitoes A Soltani 1, *H Vatandoost 1, H Jabbari 2, AR Mesdaghinia1, AH Mahvi 1, M Younesian 1, AA Hanafi-Bojd 1, S Bozorgzadeh 3, MR Abai 1, A Pakari 1, H Shabkhiz 1 1Department of Medical Entomology and Vector Control, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences, Iran 2 Research Center for Environmental Health, Tehran, Iran 3 Chabahar Free Trade Zone, Sistan and Baluchistan Province, Iran (Received 17 Nov 2008; accepted 12 May 2009) Abstract Background: Mosquitoes transmit several diseases to human. There are several measures for control of larvae. As part of Integrated Vector Management (IVM) program, the utility of floating layers of polystyrene beads (EPS) is a potential alternative in habitats of mosquito larva. EPS beads prevent oviposition of mosquito as well as killing the immature stages by forming a tick layer on the water surface. They are cheap, environmentally safe and do not need frequent application and remain on the surface of water for long time. The objective of the current study was to asses the effectiveness of two types of polystyrene beads of (EPS) and (SWAP) for control of mosquito larvae under labo- ratory conditions. Methods: Anopheles stephensi and Culex quinquefasciatus were used for experimental purposes. In each tray 250 larvae of late 3rd and early 4th instars were introduced. The experiment was conducted on 4 replicates for An. ste- phensi, Cu. quinquefasciatus and combination of both. Emerging of adult mosquitoes were calculated every day until the end of experiments. Results: Mortality rate and Inhibition of Emerge (IE) for Cu. quinquefasciatus, An. stephensi and combination of both species was 97.8%, 100% and 99.07%, respectively using EPS. In average, EPS was able to kill 98.9% of larvae. The figures with SWAP were 63%, 91.05% and 72.65%, respectively. The average mortality for mosquitoes was 75.57% Conclusion: EPS and SWAP beads can be very effective and practical for elimination of An. stephensi and Cx. quinquefasciatus under the laboratory conditions. Keywords: Polystyrene beads, Mosquitoes, Anopheles stephensi, Culex quinquefasciatus, Iran Introduction Mosquitoes belonging to the family of Culicidae transmit some of the worldwide im- portant diseases such as malaria, filariasis, yel- low fever and dengue hemorrhagic fever. These diseases are rising and some of them re-emerged in many tropical and subtropical areas (WHO 1999). Therefore, approaches to reduce the incidence of vector-borne diseases by control- ling mosquito populations are highly warranted (Subbiah and Brij Kishore 2006). Urbanization with poor sanitation increas- es the number of breeding places for mosqui- toes, especially Culex spp, such as pit latri- nes, cess pits, cesspools, disused wells, blocked open drains, open septic tanks, polluted pud- dles, uncovered overhead tanks (Das and Ra- jagopalan 1989, Nathan et al. 1996). In some parts of Iran due to the lack of water pipe system, villagers use the stored water for drink- ing and washing. These kind of breeding places have ideal conditions for Anopheles, Aedes *Corresponding author: Dr H Vatandoost, Tel/ Fax: +98 21 88951393, E-mail: hvatandoost@ yahoo.com Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 13 and Culex mosquito habitats. Re-emerge of ma- laria epidemic have occurred in the cities of Iran in the recent years (Edrissian 2006). Mosquito control with the use of insecticides in some areas is not very effective because of vector resistance, exophilic vector behavior, environ- mental concern and low community acceptance (Malaviya et al. 2006). Additionally only few number of insec- ticides have been recommended for control of mosquitoes in potable water. Many populations of mosquito vectors of diseases have devel- oped resistance to synthetic organic insecticides (organochlorines, organophosphates, carbamates and pyrethroids) that have been used mostly during the last half of the 20th century. Thus interest in alternate strategies as well as Inte- grated Vector Management (IVM) have been increased in recent years (WHO 1982a, b, WHO 1986). Therefore, methods of control are effective to produce the desired results, includ- ing the use of bio-control agents and environ- mental management. One of the effective non-chemical meth- ods for control of mosquito’s larvae is usage of expanded polystyrene (EPS). These agents eliminate/minimize the mosquito breeding places leading to decrease of adult population. An al- ternative and plentiful source of expanded po- lystyrene is the waste material destined for land- fill sites, packaging, from perishable foodstuffs and shipping electrical and other appliances. Unexpanded polystyrene beads are pro- duced by the petrochemical industry with pen- tane in solid solution in each bead. Industri- ally, the beads are heated with steam to make EPS beads, which are then heated and pres- sed to form blocks or slabs for packaging, insulation (Curtis et al. 1989, Sivagnaname et al. 2005). Also shredded waste polystyrene (SWAP) is produced from this products and a suitable alternative to use of commercially available, SWAP appears to offer an effective, cheep and readily available alternative to EPS for mosquito controlling purposes. The transport of large volumes of EPS beads over long distances is cost prohibitive and thus expansion using locally available re- sources is suggested (Curtis and Feachem 1981). A 2 cm thick layer of 2 mm beads is suf- ficient to eliminate mosquito breeding. Float- ing layers of 2 mm expanded polystyrene were found to be most effective for suffocating Ano- pheles larvae and pupae. They must be placed in sacks or dumps to take them to the pits where they are needed. If piled loose in an open trunk, they would blow away before reach- ing their destination. Storage of beads for quite a long time would result in failure of the beads to expand to the maximum when boiled due to loss of pen- tane; hence it has been suggested that beads should be boiled soon after procurement from the production firm (Madurai and Nadu 1995). In Iran there are several methods for control of mosquito larvae in breeding places. Use of some organophosphate insecticides, pat- chy application of Bacillus thuringiensis, and introducing of larvivorus fishes are the main control measures. The bionomics of malaria ve- ctors have been studied in the region (Vatan- doost and Moinvaziri 2004, Vatandoost et al. 2004, Vatandoost et al. 2006, Hanafi-Bojd et al. 2006). The objective of the present study was to evaluate the efficacy of local made indus- trial beads for mosquito control under labo- ratory conditions. Material and Methods Mosquito strains An. stephensi is the main malaria vec- tor in Hormozgan Province. The filed strain of Bandar Abbas was collected and reared in the insectray of Bandar Abbas Health Research Center, southern Iran related to Tehran Univer- sity of Medical Sciences, School of Public Health. Cu. quinquefasciatus also was collected from the field and maintained in the insectary. Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 14 EPS and WASP EPS and SWAP were purchased from The National Petrochemical Company (NPC), in Iran. Experimental design Sample size was designed according to WHO methods (WHO 2005) with some mi- nor modifications. A total of 36 trays of 35× 25×10 cm were used. A total of 250 larvae of mosquito (250 Anopheles or 250 Culex or mixed 125 Culex+125 Anopheles, based on designed pattern) at the late 3rd and early 4th instars were released into each tray. Based on kind of treatment, four replicates were used for each species (Fig. 1). Temperature in the insectaria was 27±3o C, with a period of 14-10 h light-dark. Relative humidity was maintained at 70-80%. Larvae were fed by fish flakes. Feeding and water volume were pre- pared for all trays as required. Subsequently the EPS and SWAP were added to the water surface of treated trays to make a layer of 0.5-1 cm. One group of 1000 larvae was used as control for each species. Fig. 1 shows the de- sign of the experiment. Group1 Group (II) Group (III) Anopheles : Culex Anopheles + Culex SWAP EPS Control Fig. 1. Schematic diagram of the tests performance The fish food was added daily to the rearing trays, and then the surface of treated water was covered with the EPS or SWAP. The rearing trays were covered with net to prevent the adult mosquito escaping during the test period. Mosquitoes were emerged daily and trapped under the net cover. This allowed us to catch them using a mouth aspirator and to identify the genus. Adult emergence was cal- culated daily up to 20 d. At the end of experi- ments all the dead and alive stages of mosqui- toes were counted and recorded. After emerging all mosquitoes, the dead adults, pupae and larvae from the polystyrene layers were counted and subjected to statistical analysis. Statistical analysis The larval mortality data were corrected according control mortality by the Abbott’s formula (Abbot 1925). Inhibition of emerge (IE%) or mortality of mosquitoes is calculated using the following formula: Where T= percentage emergence in treated trays and C= percentage emergence in the control. If adult emergence in the control is less than 80%, the test should be discarded and repeated (Mulla and Darwazeh 1975). Where the percentage is between 80% and 95%, the data are corrected using Abbott’s formula. ANOVA was carried out with SPSS 11.5. Means were compared with Post Hoc Tests (LSD), (P< 0.05). Results Results of mosquitoes mortality based on life stages are given in Table 1. From a total of 5750 out of 9000 larvae in all rearing trays, the highest number of mortality (2973) was recorded in EPS, followed by SWAP (2362), and control (415) rearing trays. Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 15 If the control mortality was between 5% and 20%, the mortalities of treated sam- ples were corrected according to Abbott’s formula. Results of adult mosquitoes collected live using mouth aspirator based on used ma- terials, are showed in Fig. 2 during 20 d of the study period. We can observe differences be- tween three methods clearly. In another step of the experiment, we assessed and compared the survival means by Post Hoc Tests (LSD) based on untreated con- trol and treated samples. Details of daily emer- gence based on mosquito groups and the tested materials are presented in Fig. 2. As shown in the Table1, the adult emergence in the control is 86.17% (mortality 13.83%), therefore our treatment results were corrected using the Abbott's formula. Fig. 3 has compared per- centage emergence of mosquitoes with 95% confidence intervals for each types of polysty- rene, species of mosquitoes and control group. Table 1. Mortality of mosquitoes in treated samples and untreated control EPS group Mosquito species Larva Pupa Adult Total %Mr Corrected Mr(IE%) * Culex 981 0 0 981/1000 98.1 97.8 Anopheles 998 2 0 1000/1000 100 100 Anopheles+Culex 984 8 0 992/1000 99.2 99.07 Total 2963 10 0 2973/3000 99.1 98.96 SWAP group Mosquito species Larva Pupa Adult Total %Mr Corrected Mr * Culex 379 25 269 673/1000 67.3 63.0 Anopheles 549 83 293 925/1000 92.5 91.05 Anopheles+Culex 402 26 336 764/1000 76.4 72.65 Total 1330 134 898 2362/3000 78.73 75.57 Control group Mosquito species Larva Pupa Adult Total %Mr Culex 109 7 0 116/1000 11.6 Anopheles 146 16 0 162/1000 16.2 Anopheles+Culex 137 0 0 137/1000 13.7 Total 392 23 0 415/3000 13.83 * Percentage of mortality (IE%) is corrected by Abbott’s formula Mr: Mortality 0 100 200 300 400 500 600 700 800 900 1000 DAY 1 DAY 2 DAY 3 DAY 4 DAY 5 DAY 6 DAY 7 DAY 8 DAY 9 DAY 10 DAY 11 DAY 12 DAY 13 DAY 14 DAY 15 DAY 16 DAY 17 DAY 18 DAY 19 DAY 20 Control SWAP EPS Fig. 2.Number of mosquitoes collected by aspirator from different rearing trays in treated and control experiments Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 16 0 0.8 86.3 23.6 83.8 7.5 1.9 32.7 88.4 0 10 20 30 40 50 60 70 80 90 100 EPS SWAP Control EPS SWAP Control EPS SWAP Control Culex Anopheles Anopheles + Culex a a b a b c b c c The mean difference is significant at the 0.05 level Fig. 3. Comparing the emergence of mosquitoes in two types of polystyrene Discussion There are several reports concerning use of polystyrene beads for elimination of mos- quito emergence in the world in the cess pits, flooded cellars, soakage pits, water tanks, gem pits and industrial complex (Rietre 1978, Cha- vasse et al. 1995, Curtis and Minjas 1985, Curtis 1994, Maxwell et al. 1990, Reuben et al. 2001, Chandrahas and Sharma 1987, Yapa- bandara and Curtis 2002, Dua et al. 1989, Rieter 1985, Sharma 1985, Chandrahas et al. 1993). The layer of this product reduces the density of mosquito by suffocating larvae and pupae and inhibiting mosquito egg-laying in water surface. Our results showed that utility of EPS and SWAP beads can be completely effec- tive and practical for elimination of An. ste- phensi and Cx. quinquefasciatus under the laboratory conditions. Larval mortalities in- creased significantly (P< 0.05) with using both polystyrene forms and the mortality was greater in those larvae treated by EPS than SWAP (P> 0.05). Mortality rate for Cx. quin- quefasciatus, An.stephensi and combination of both species was 97.8%, 100% and 99.07%, respectively using EPS. In average EPS was able to kill 98.9% of mosquitoes. Observa- tions of the present study show that EPS beads induced 98.9% mortality in the tested mos- quitoes, representing 98.61% and 0.34% in the larval and pupal stages, respectively. The fig- ures for SWAP were 63%, 91.05% and 72.65%. The average mortality for mosquitoes was 75.57%. The SWAP beads caused 75.57% mortality: 42.55% larvae, 4.29% pupae and 28.73% in newly emerged adults that were trapped in SWAP beads. Inhibition of Emerge (IE) for EPS ob- tained 100%, 97.8 and 99.07 in the case of An. stephensi, Cx. quinquefasciatus and An. ste- % E m er ge nc e Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 17 phensi+Cx. quinquefasciatus, respectively. The IE for SWAP was calculated 91.05, 63.0 and 72.65 in An.stephensi, Cx. quinquefasciatus and An.stephensi+Cx. quinquefasciatus, re- spectively. Similar studies showed a mortal- ity rate of 72.3-100% (Curtis et al. 1989, Si- vagnaname 2005). The observed mortality using EPS im- ply that this material provides an appropriate and unique floating layer on the water sur- face, however the SWAP beads due to its variability in beads’ diameter and irregular sha- pes are not able to cover water surface com- pletely, mortality and inhibition of emerge is less than that of EPS. This factor leads to adult emergence in SWAP treated trays. Although a relatively high number of mos- quito larvae could converted to adults, but most of them were trapped among layers of SWAP beads consequence of trapping and dead. Some of emerging adults showed mor- phological abnormalities such as wing, leg, head, thorax and abdomen malformations at rearing trays treated by SWAP. Difference in survival rate of Anophe- les and Culex mosquitoes is another impor- tant point. The emergence rate for An. ste- phensi was 46.31%, among which 22.49% of them were trapped among SWAP beads and resulted to dead, so the survival rate for this species was evaluated as 23.82%. Concern- ing Cx. quinquefasciatus, 75.91% of larvae completed their development cycle and chan- ged to adult stage, while 39.27% of them were dead and therefore the survival rate was calculated as 36.27%. The comparison between the survival rates of these two mos- quito species showed that Culex has more rela- tive ability to survive than Anopheles (1.5- fold). This ability may be is attributed to the morphological and physiological characteris- tics and respiratory system of the Culex larvae that allow them to breathe easier than Ano- pheles under the covering layers on water sur- face. Additionally Culex mosquitoes are able to survive in organic polluted water bodies. Par- allel to our study, Nathan et al. (1996) con- ducted laboratory and filed trail on the use of commercially available, expanded-polystyrene beads for the control of Cx. quinquefascia- tus. They were not able to gain 100% mor- tality in the population of mosquitoes in the laboratory. They concluded that the irregular shape of the SWAP particles greatly reduced their capacity to spread over the water surface and the interstitial air spaces also permitted respiration and development of mosquito lar- vae. Under field conditions and in wet pit la- trines the control sustained for two months. Efficacy of Agnique MMF, a monomo- lecular film formulation, was tested against im- mature of An. stephensi in India. Simulated field trials carried out in cement tanks showed 100% inhibition of adult emergence for up to 3 weeks. In tanks and wells it produced 75% reduction of late instars and 100% reduction of pupae on day 1 (Batra et al. 2006). Beehler and DeFoliart (1991) used EPS for control of Aedes triseriatus in the labora- tory and the field. EPS reduced the emergence of adults by preventing normal eclosion from the pupae. In the field, tree holes containing EPS had significantly fewer larvae than controls. Bekheit et al. (1991) compared the oils and expanded polystyrene beads for the con- trol of mosquito breeding in Egypt. Results re- vealed the superiority in effectiveness of beads over oil for the control of mosquito breeding. Mosquito catches from cesspits treated with polystyrene beads dropped to zero soon after application of the beads and remained so throughout five months observation period. A field trial of the use of EPS to control the breeding of mosquito larvae in household septic tanks was conducted by Chang et al. (1995). One week after treatment, the breeding of Cx. quinquefasciatus and Ae. albopictus was reduced by 100% and 68.7%, respec- tively. For both species combined, a 57.25% reduction in the adult emergence rate was achi- eved. No adult was caught in the emergence trap one month after treatment. Iranian J Arthropod-Borne Dis, (2008), 2(2): 12-20 A Soltani et al: Use of Expanded Polystyrene… 18 Data analysis of our results for mosquito’s survival showed that there are significant dif- ferences between two polystyrene forms (P< 0.05). Using EPS, a significant difference ob- served between all mosquitoes (Anopheles, Culex, and their combination) as compared to the control group (P< 0.05), additionally there was significant difference between two species (P= 0.012). Similarly, in the SWAP tests a significant difference observed between all spe- cies and control (P< 0.05). Therefore, the pre- sent study clearly indicates that EPS beads could provide excellent larval control for mos- quitoes. In addition, it was found that both materials had high larval control and both of them could be used in the control of mosqui- toes. EPS beads could be used as one of the promising vector control methods to eliminate/ minimize the mosquito breeding sources in IVM programs, while SWAP appears to offer good prospects as an alternative to expanded-poly- styrene beads for inexpensive, long-term mos- quito control in selected habitats. In total, regardless of efficacy of the method in the control of mosquito larval stage under laboratory conditions, the extensive field stud- ies in the area (Iran) are recommended to eva- luate and may be introduce this method as an easy and inexpensive way in mosquito control, especially in non-drinking water sources. Trials of using polystyrene beads for control of mosquito have been conducted in several parts of the world (Curtis et al. 2002). The method greatly and substantially reduced the vector population. Utility of EPS and SWAP as a component of Integrated Vector Manage- ment with other supportive measures could as- sist considerably the process of elimination of mosquito-borne disease. Further study is requ- ired to the practicality of using these products in community-based, mosquito-control programs. Acknowledgements Authors are grateful to Center for En- vironmental Research (CER), Tehran Uni- versity of Medical Sciences, for financial support of this research project. 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