J Arthropod-Borne Dis, December 2016, 10(4): 595–601 S Koc et al.: Evaluation of Some Plant … 595 http://jad.tums.ac.ir Published Online: October 04, 2016 Short Communication Evaluation of Some Plant Fruit Extracts for the Control of West Nile Virus Vector Culex pipiens (Diptera: Culicidae) Samed Koc, Ozay Hasan Evren, *Huseyin Cetin Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey (Received 22 Feb 2015; accepted 12 Oct 2015) Abstract Background: The extracts of different parts of plants were found very effective against various pests. The aim of this research was to determine the insecticidal activity of fruit methanol extracts obtained from Melia azedarach (Meliaceae), Phoenix theophrasti (Arecaceae), Styphnolobium japonicum (Fabaceae) and Pyracantha coccinea (Rosaceae) against the larvae of Culex pipiens (Diptera: Culicidae). Methods: The fruits of test plants were collected from the Campus of Akdeniz University, Antalya, Turkey in 2013. A series of concentrations of the extracts ranging from 62.5–1000 ppm were tested against second instar larvae. Results: Only the extracts of Me. azedarach and Ph. theoprasti showed significant larvicidal activity against Cx. pipiens and the LC50 values of these extracts were found to be 169.48 and 220.60 ppm, respectively. This is the first research investigating the insecticidal or larvicidal activity of Ph. theophrasti, St. japonicum and Py. coccinea ex- tracts on mosquitoes. Conclusion: The methanol extract of fruits of Me. azedarach and Ph. theophrasti showed significantly higher larvi- cidal activity against Cx. pipiens. Keywords: Larvicidal, Melia azedarach, Phoenix theophrasti, Pyracantha coccinea, Styphnolobium japonicum Introduction Mosquitoes are important organisms known being vectors of many fatal or neurologic dis- eases such as malaria, dengue and West Nile Virus (WNV) infections. Fortunately, most people infected with some of these diseases have no or develop a fever with other symp- toms. However, mosquitoes know no borders and more than one million people worldwide die from mosquito borne diseases every year (Becker 2008). Some insecticide groups can be applied to control adults (adulticides) or lar- vae (larvicides) of mosquitoes. Insecticides used for larviciding include, chitin synthesis inhibitors, diflubenzuron, novaluron and tri- flumuron, juvenile hormone analogs pyri - proxyfen and methoprene, bacterial products (Bacillus thuringiensis subsp israelensis and B. sphaericus), spinosyns (spinosad) (Cetin et al. 2005, 2006, 2007). These larvicides were highly effective against different mosquito species in the genus of Anopheles, Culex and Aedes but in some conditions some of them have negative effects on non-target organ- isms (Lawler et al. 1999, Ser and Cetin 2015). In addition, many researchers observed re- sistance toward larvicides in field popula- tions (Sharma et al. 2003, Wirth 2010). Researches about botanical insecticides and acaricides have grown dramatically in recent years and essential oils and extracts of aromatic plants were found effective against different instars of arthropod pest species (Koc et al. 2012). Most plant essential oils and extracts obtained from flowers, fruits and leaves are complex mixtures that contain ac- tive constituents such as alcohols, aldehydes, esters, ketones, phenols and terpenes (Gu et al. 2009). The use of botanical compounds *Corresponding authors: Dr Huseyin Cetin, E- mail: hcetin@akdeniz.edu.tr J Arthropod-Borne Dis, December 2016, 10(4): 595–601 S Koc et al.: Evaluation of Some Plant … 596 http://jad.tums.ac.ir Published Online: October 04, 2016 extracted from aromatic plants may be an al- ternative to conventionally used insecticides to control of mosquitoes (Sukumar et al. 1991). Therefore, in this study the toxic effects of fruit extracts of four plant species; Melia azedarach L (Meliaceae), Phoenix theophrasti Greuter (Arecaceae), Styphnolobium japoni- cum (L) (Fabaceae) and Pyracantha coc- cinea Max Joseph Roemer (Rosaceae) were investigated against larvae of Culex pipiens L (Diptera: Culicidae). This mosquito is found worldwide and is vector of different patho- genic organisms that cause serious diseases (i.e. WNV and filariasis). In Turkey, Cx. pipiens is abundant in many cities and de- veloped resistance to some insecticide groups. Materials and Methods Plant materials and their extractions Tested plants, Me. azedarach, Ph. the- ophrasti, St. japonicum and Py. coccinea were taxonomically identified by the second author. The fruits of them were collected from the Campus of Akdeniz University, Antalya, Turkey in 2013. Fruit samples were deposited in the Insecticide Test Laboratory of Biology Department, Faculty of Science, Akdeniz University. Fruits of each species were separated from their stalks. After that, fruit materials were dried at 25 °C about 2 weeks and ground to fine powder using blend- er. Extractions of the samples were carried out by using methanol for 2 days at 25 °C. Then filtered using a Whatman No.1 filter pa- per and dried under vacuum rotary evaporator. Target mosquito species Culex pipiens used in the studies origi- nated from Arapsuyu, Antalya, and was col- lected from a pool in August 2011. The lar- vae were reared at 12 h dark: 12 h light photoperiod, 60±10% relative humidity, and 26±2 °C temperature in an insectary in the Biology Department, Akdeniz University. The second instars larvae were used for bioassays. Experiments of larvicidal activities Larvicidal activity of the methanol ex- tracts of Me. azedarach, Ph. theophrasti, St. japonicum and Py. coccinea against Cx. pipiens was assessed (Oz et al. 2013). For experiments, first 2 gr of each extracts were dissolved in 25 ml distilled water. Then a series five concentrations (62.5, 125, 250, 500 and 1000 ppm) were prepared and con- trols in 500 ml tap water in containers. After approximately 5 min, 10 larvae taken on an egg tray with water were transferred gently to the test medium by tapping. Four repli- cates of each concentrations and controls were run at a time. Mortality was recorded after 24-, 48- 72- and 96-h of exposure, during which pellet fish food was given to the larvae. All experiments were conducted at 26±2 °C and 60±10% relative humidity with 12 h dark: 12 h light photoperiod. Dead larvae were identified when they failed to move after probing with a needle in the si- phon or cervical region. Moribund larvae were those incapable of rising to the surface (within a reasonable period) or showing the characteristic diving reaction when the water was disturbed. Larvae were also observed for discoloration, unnatural positions, unco- ordination or rigor. Statistical analyses Corrected means of percentage mortalities were calculated using Abbott`s formula. The percentage values were transformed to en- sure normality and variance homogeneity using an arcsine transformation. The data was sub- jected to analysis of variance (ANOVA) and the means compared with Duncan’s multiple range tests (P< 0.05). The LC50 and LC90 values were calculated from percent mortal- ity data by using probit analysis (Finney 1971). Results The percent mortality values of four plant fruit methanol extracts against second J Arthropod-Borne Dis, December 2016, 10(4): 595–601 S Koc et al.: Evaluation of Some Plant … 597 http://jad.tums.ac.ir Published Online: October 04, 2016 instar larvae of Cx. pipiens are shown in Table 1. In our experiments, low mortalities were observed in the extracts of St. japoni- cum (14.45%) and Py. coccinea (6.42%) after 96 h exposure. These results are not significantly different from the controls (P< 0.05) (Table 1). The highest mortalities were achieved by Me. azedarach and Ph. theoprasti. The Me. azedarach extract caused 100% mortality at the concentration of 1000 ppm after 96 h ex- posure. Ph. theoprasti also demonstrated sig- nificant larvicidal activity and in the same concentration, mortality rate was 83.49%. There was a remarkable increase in mortality (19.95 to 83.49%) when concentrations were increased (62.5 to 1000 ppm) (P< 0.05). For 125 ppm of the extracts of Me. azedarach and Ph. theoprasti more than 39% larvae died only after 96 h of exposure (Table 1). The LC50 values against the second instar larvae of Cx. pipiens were 220.60 ppm for Ph. theophrasti extract, 169.48 ppm for the Me. azedarach extract (Table 2) (Fig. 1). Fig. 1. Concentration-response lines for most effective test extracts J Arthropod-Borne Dis, December 2016, 10(4): 595–601 S Koc et al.: Evaluation of Some Plant … 598 http://jad.tums.ac.ir Published Online: October 04, 2016 Table 1. Larvicidal activity of the fruit extracts of Phoenix theophrasti, Melia azedarach, Styphnolobium japonicum and Pyracantha coccinea (Percent mortalities ± Standard Errors) Concentrations (ppm) Times After Exposure (hours) 62.5 125 250 500 1000 Control Phoenix theophrasti 24 0±0 aα, Aβ 0±0 a, A 0±0 a, A 0±0 a, A 5.0±2.89 b, A 0.83±0.83 a, A 48 0±0 a, A 2.5±2.5 a, A 2.5±2.5 a, A 5.0±5.0 a, A 5.0±2.89 a A 1.67±1.12 a, A 72 7.5±4.79 a, AB 27.5±11.09 b, AB 27.5±10.31 b, B 35.0±6.45 b, B 40.0±4.08 b, B 3.33±1.88 a, A 96 19.95±7.14 a, B 42.2±12.21 b, B 53.21±2.75 bc, C 66.97±4.49 c, C 83.49±3.18 d, C 9.17±2.6 a, B Melia azedarach 24 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0.83±0.83 a, A 48 7.5±4.79 a, A 0±0 a, A 7.5±4.79 a, A 20.0±4.08 b, AB 20.0±7.07 b, A 1.67±1.12 a, A 72 27.5±14.36 ab, A 25±11.9 ab, AB 32.5±6.29 ab, B 40±12.25 b, B 80±7.07 c, B 3.33±1.88 a, A 96 33.94±11.89 ab, A 39.45±17.12 bc, B 53.21±9.4 bc, C 66.97±7.79 c, C 100±0 d, C 9.17±2.6 a, B Stypnolobium japonicum 24 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0.83±0.83 a, A 48 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 1.67±1.12 a, A 72 0±0 a, A 2.5±2.5 ab, A 0±0 a, A 5.0±2.89 ab, A 10.0±5.77 b, A 3.33±1.88 ab, A 96 2.98±2.98 a, A 3.21±2.91 a, A 5.96±3.44 a, B 3.21±2.91 a, A 14.45±7.08 a, A 9.17±2.6 a, B Pyracantha coccinea 24 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 0.83±0.83 a, A 48 0±0 a, A 0±0 a, A 0±0 a, A 0±0 a, A 2.5±2.5 a, A 1.67±1.12 a, A 72 5.0±2.89 a, B 2.5±2.5 a, A 5.0±2.89 a, A 5.0±2.89 a, A 10.0±4.08 a, A 3.33±1.88 a, A 96 0.46±0.26 a, A 0.46±0.26 a, A 6.19±3.31 a, A 6.42±3.18 a, A 6.42±5.51 a, A 9.17±2.6 a, B α: Means within a line followed by the same lower case letter are not significantly different Dun- can’s multiple range test (P< 0.05). β: Means within a column followed by the same capital letter are not significantly different Dun- can’s multiple range test (P< 0.05). J Arthropod-Borne Dis, December 2016, 10(4): 595–601 S Koc et al.: Evaluation of Some Plant … 599 http://jad.tums.ac.ir Published Online: October 04, 2016 Table 2. The Lethal Concentration 50 and Lethal Concentration 90 values (ppm) of fruit extracts of Phoenix theophrasti, Melia azedarach, Styphnolobium japonicum and Pyracantha coccinea on Culex pipiens second-instar larvae in the laboratory conditions Plant species LC50 LC90 x 2 P-value Ph. theophrasti 220.60 1813.67 1.42 0.69 Me. azedarach 169.48 1098.82 6.84 0.07 St. japonicum 39054.36 1792194.42 3.50 0.32 Py. coccinea 32110.12 808652.32 6.19 0.10 Discussion The calculated LC50’s were higher or lower to other comparable studies on the toxicity of Me. azedarach to mosquitoes. These differences may be attributed to many factors such as, method of extraction (ex- traction solvent, extraction time, extraction temperature), parts of plant (flowers, leaves, fruits), vegetation period, origin of the pest species. The higher LC50 values were deter- mined for St. japonicum and Py. coccinea extracts, since significant mortalities were not determined even at the highest exposure concentration (1000 ppm) (Table 2). The Meliaceae family has been known as a source for pesticides and the efficacy of neem products on different pest species was reported (Singh et al. 2006, Ndione et al. 2007). Melia azedarach has insecticidal ef- fects on different mosquito genus including Aedes and Culex (Wandscheer et al. 2004, Ndione et al. 2007). The LC50 value of aque- ous extracts of fruits was 2035 ppm for Cu. quinquefasciatus Say third and fourth instar larvae (Ilahi et al. 2012). During our re- search the lowest dose of fruit extract (62.5 ppm) caused 33.94% mortality and the high- est concentration (1000 ppm) caused 100% mortality. Mode of action of Me. azedarach extract on mosquito larvae may explain that extract causing serious damage to the gastro intestinal system cells. RM Al-Mehmadi and Al-Khalaf (2010) showed that changes in the midgut, included separation of the epithelial cells from the basement membrane with dam- age of the peritrophic membrane causes that the mixing of the gut contents with the he- molymph caused the mortality. Fruit extracts of this species have also different effects on insects such as changes of behavior, anti- feedant, inhibit the chitin or hormone syn- thesis, reduced fecundity etc. (Schmidt et al. 1998, Gajmer et al. 2002). According to our literature survey, no re- searches have been carried out so far on the insecticidal properties of Ph. theophrasti, St. japonicum and Py. coccinea against pest or- ganisms. To our knowledge, this is the first research showed the insecticidal or larvicidal activity of Ph. theophrasti against Cx. pipiens. Fruit extracts of Ph. theophrasti caused high mortalities as much as Me. azedarach extract except the highest concentration (P< 0.05). A series of experiments aimed at clarifying the mode of action of Ph. theophrasti should be done on mosquito larvae. The various solvent extracts (e.g. acetone, methanol, chloroform) of different parts (flow- ers, leaves, fruits) of many plant species were found very effective against various pests (Kal- yanasundaram and Das 1985, Govindarajan and Sivakumar 2014). Most of them contain active components of which have toxic ef- fects on insect biology (Cecilia et al. 2014). Conclusion The methanol extract of fruits of Me. azedarach and Ph. theophrasti showed sig- J Arthropod-Borne Dis, December 2016, 10(4): 595–601 S Koc et al.: Evaluation of Some Plant … 600 http://jad.tums.ac.ir Published Online: October 04, 2016 nificantly higher larvicidal activity against Cx. pipiens. Our results suggest that the fruit extracts of Ph. theophrasti have the potential to be used as an alternative product for the control of mosquitoes. 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