Hamid F.doc J Bagh College Dentistry Vol. 25(4), December 2013 The effect of smear Restorative Dentistry 5 The effect of smear layer on push-out bond strength to dentin of Bioceramic sealer (In vitro study) Hamid Abbas Hamid, B.D.S. (1) Abdul-kareem Jassim Al-Azzawi, B.D.S., M.Sc. (2) ABSTRACT Background: Sealers should demonstrate adhesive properties to dentin, decreasing the chance of endodontic treatment failure. Increased adhesive properties to dentin may lead to greater strength of the restored tooth, which may provide greater resistance to tooth fracture and clinical longevity of an endodontically treated tooth. The aim of this study was to evaluate the shear bond strength of Bioceramic iRoot SP sealer, AH plus sealer and Apexit plus sealer in absence or presence of smear layer using push out bond strength test. Materials and Methods: Sixty straight single roots of the mandibular premolars were selected for this study. All canals were instrumented using ProTaper rotary instruments to achieve tapered canal walls, instrumentation was done with copious irrigation of 5.25% sodium hypochlorite. Roots were randomly divided into three groups according to the type of sealer used (twenty teeth for each group): Group A: Apexit plus + gutta percha, Group B: AH plus sealer + gutta percha, Group C: iRoot SP sealer + gutta percha .Then groups were subdivided according to types of final irrigation into two subgroups. Groups (A1, B1, and C1) were irrigated with 5 ml of 5.25% NaOCl for 1 minute while Groups (A2, B2, and C2), the smear layer was removed with 5 ml of 17% EDTA for 1 minute. All groups were rinsed with distilled water and then obturated with cold lateral condensation technique, the roots then stored in moist environment at 37°C for one week. The roots were embedded in clear acrylic resin and three horizontal sections were prepared at a thickness of 1 mm ±0.1 in the apical, middle and coronal parts of each root. The test specimens were subjected to the push-out test method using a Universal Test Machine that carried 1-mm, 0.5- mm and 0.3-mm plungers for coronal, middle and apical specimens, respectively. The loading speed was 0.5 mm/ min. The computer showed the higher bond force before dislodgment of the filling material. These forces were divided by the surface area to obtain the bond strength in MPa. Results: The results showed that the bond strengths of iRoot SP and AH Plus were significantly higher than those of Apexit plus, but there was no significant difference between the bond strength of iRoot SP and AH Plus.In terms of root segments, the bond strengths in the middle specimens and the apical specimens were higher compared with the bond strengths in the coronal specimens. Conclusion: The presence or absence of smear layer did not significantly affect the bond strength of Bioceramic filling materials. Keywords: Bioceramic sealer, smear layer, push out test. (J Bagh Coll Dentistry 2013; 25(4):5-11). INTRODUCTION The introduction of bioceramic technology is considered a dramatic change in endodontic obturation. The introduced iRoot SP (Innovative Bioceramix, Vancouver, Canada) is a premixed, ready-to-use injectable and hydrophilic cement paste. It is composed of calcium phosphate, calcium silicate, calcium hydroxide, zirconium oxide, filler, and thickening agents. One of its advantages is its ability to form hydroxyapatite during the setting process and ultimately create a bond between dentinal wall and the sealer. It has been shown that iRoot SP is equivalent to AH Plus sealer in apical sealing ability, Furthermore, it was demonstrated that I Root SP was significantly less toxic than AH Plus 1,2. Instrumentation of root canals produces a smear layer consisting of inorganic and organic components. The mechanical interlocking of the sealer plug inside the tubules following smear layer removal has been suggested to improve retention of the material, which might improve the sealing ability 3. (1) M.Sc. Student, Department of Conservative Dentistry, College of Dentistry, University of Baghdad. (2) Professor, Department of Conservative Dentistry, College of Dentistry, University of Baghdad. On the other hand, it has been shown that the bond strength of some sealer cements to dentin was better in the presence of smear layer 4. Furthermore, because the smear layer contains moisture and might act as a coupling agent, thereby improving the adaptation of hydrophilic materials to the root canal wall. The removal of smear layer might have a negative effect on hydrophilic root canal sealers such as BC Sealer 5. The push-out test provides a better evaluation of bonding strength than the conventional shear test; because when using the push-out test, fracture occurs parallel to the dentine–bonding interface, which makes it a true shear test for parallel-sided samples 6,7. Interfacial strength and dislocation resistance between the root filling material and the intra-radicular dentine have been evaluated using thin-slice push-out tests 8-10. MATERIALS AND METHODS Sixty freshly extracted mandibular premolars with straight single roots and close apices were used in this study. The age of patients range between (18-48) years but the reason of extraction and gender was not considered. After extraction, all teeth were stored in 0.1% thymol solution at J Bagh College Dentistry Vol. 25(4), December 2013 The effect of smear Restorative Dentistry 6 room temperature. Any periodontal remnants or soft tissues were removed by periodontal curette and root surfaces were verified with magnified eye lens (10X) and light cure device for any defects and cracks. After the length of root was determined by digital calliper and marker to 14 mm from apex to cemento-enamel junction, the root was sectioned perpendicular to its long axis by using diamond disc in a straight hand piece with water coolant to facilitate straight line access for canal preparation and filling procedure, also to eliminate the variables in access preparation and get flat reference point for measurement 11. The pulpal tissue was removed by using barbed broach and copious amount irrigation of 5.25% NaOCl. The potency of canals was verified by insertion of No.15 K file into canal until it was visualised at apical foramen. The exact working length was established by subtracting 1mm from this measurement which is 13 mm. A Silicon rubber base (heavy-body) was mixed (base and catalyst) according to manufacturer instruction and inserted in plastic containers then the sectioned root was inserted inside the rubber base. Heavy body was left to set forming a small block to facilitate handling of the roots during instrumentation and obturation technique. The roots were instrumented by Rotary ProTaper (NiTi) system from SX-F3. All instrumentation was carried out according to manufacturer's instructions and completed in a crown-down manner using a gentle in-and-out motion. Instruments were withdrawn when resistance was felt and changed for the next instrument. The root was flooded with 5 ml of 5.25%NaOCl solution delivered with needle tip gauge 27 placed within apical third passively without bending and washed after each file. Sample grouping The roots were randomly divided into three groups (n=20) according to types of root canal sealer used: Group A. Apexit plus root canal sealer obturation. Group B. AH plus root canal sealer obturation. Group C. I root sp root canal sealer obturation. Then each group was subdivided into two subgroups (n=10) according to the method of final irrigation. Sub groups A1, B1 and C1; The roots canals were irrigated with 5 ml of 5.25% NaOCl for one minute and then irrigated with 5 ml of distilled water.Sub groups A2, B2 and C2; The root canals were irrigated with 5 ml of 17% of EDTA for one minute and 5 ml of 5.25%of NaOCl one minute and then irrigated with 5ml of distilled water. All groups were obturated by lateral condensation technique. Group A: The samples were obturated with gutta percha and Apexit plus root canal sealer. The sealer was mixed according to the manufactures instructions by one press on handle of cartridge, equal amounts of base and activator were dispersed and mixed on clean and dry glass slab. The mixture had homogenous creamy consistency that stringed out when elevated with cement spatula over glass slab for one inch. Each canal was dried with paper point size F3. The K type master cone of gutta percha size 30 was adjusted to working length with tug back. Protaper absorbent paper point size F3 was dipped in sealer and coated the canal walls by counter clock wise rotation. The tip of master gutta percha cone was dipped into the sealer and inserted to correct full working length. The previously checked finger spreader size 20 was inserted between master cone and the canal wall using firm (apical only) pressure to within (1-2mm) from working length. The spreader was moved apically with a 180⁰ clockwise-anticlockwise movement. The tapering of spreader was a mechanical force that laterally compresses and spread gutta percha creating space for additional accessory cones size 15 and 20. When the spreader did not inter more than 2mm ,excess gutta percha was removed with heated instrument to level 1mm higher than the coronal end of the root and vertically condensed with root canal plugger so gutta percha was obturated the entire canal up to canal terminus. The load applied during condensation ranged from 1.5-2 Kg determined by weight balance 12. Group B: The samples in this group were obturated with gutta percha and AH plus root canal sealer. AH plus sealer was mixed according to the manufactures instructions, by mixing equal amounts (1:1) of paste A and paste B on glass slab with spatula. The mixture had homogenous consistency that stringed out at least 1 inch when spatula was raised slowly from glass slab and then the canals were obturated with the same manner used for group A. Group C: The samples in this group were obturated with gutta percha and iRoot sp sealer (Bioceramic sealer). After removing the syringe cap, attached an intra canal tip securely. Insert the tip of the syringe into apical third of root canal, filling the root canal while withdrawing the intra canal tip and then place gutta percha points inside the root canal then complete obturation in same manner in previous groups. After obturating the teeth samples of all groups, the gutta-percha was removed at 1 mm below the orifice. Then the canal orifice was J Bagh College Dentistry Vol. 25(4), December 2013 The effect of smear Restorative Dentistry 7 sealed with glass inomer cement as temporary filling to serve as a barrier to the ingress of fluids. All obturated roots of all groups were removed from impression material and wrapped in saline moistened gauze in closed plastic vial allowing the sealer to set for 7 days at 37°C in an incubator 13,14. Then the roots were embedded in clear acrylic resin 15. Metal frame of (length 70mm, width 60mm and height 30mm) containing three cylindrical holes of (diameter 12 mm * height 25mm) was used into which the prepared acrylic was loaded. Before loading the mold with acrylic, the coronal end of the roots was fixed on the face of the end rod of the dental surveyor with a sticky wax. With the aid of dental surveyor the roots were centrally located within the acrylic blocks to ensure that the sectioning would be perpendicular to the long axis of the roots.The acrylic was prepared by mixing powder and liquid in a porcelain jar. The material was left undisturbed for few minutes until it reached the workable stage and loaded into the metal mold, the rod of the surveyor with the root fixed on its face was pushed into the acrylic with gentle pressure to allow the complete embedding of the root into the acrylic and to allow the escape of the excess material. The metal frame was taken from surveyor and the material was allowed to cure under cool water at 20°C, which was necessary to compensate for the anticipated rise in the temperature of the samples subsequent to the exothermic curing reaction of the cold cure resin. The acrylic blocks were allowed to cure completely for at least 30min as recommended by the manufacturers 16. Root sectioning was done After complete curing of the acrylic mold, the metal mold was open. The excess acrylic was cut off using diamond disk mounted on straight hand piece and engine with a rotation speed regulator, the hand piece was fixed in a cutting device. The root was cut horizontally with flow cold water (19-25°C) to minimize smearing 16. To get three sections of 1mm in thickness coronal, middle and apical, the cuts were made at 2,6,9 mm from coronal reference point respectively. Push-out test was performed by applying a compressive load to the apical aspect of each slice via a cylindrical plunger mounted on Tinius-Olsen Universal Testing Machine managed by computer software. Samples were examined under the Nikon metallurgical microscope (magnification 50X) and pictures of both sides of each section are taken with digital camera which was connected with microscope, and measurements calculated using LUCIA G software analysis program . The obturated area of the section at each level was measured from the apical side to determine the size of punch pin 17. Three different sizes of punch pins were used, 1 mm, 0.6mm, and 0.3mm diameter for the coronal, middle and apical slices respectively. The punch pins should provide almost complete coverage over the main cone without touching the canal walls and sealer 13,17. The root filling in each section subjected to loading using a universal testing machine (WDW50) at a speed of 0.5 mm / min in an apical-coronal direction until the first dislodgment of obturating material and a sudden drop along the load deflection. The maximum failure load was recorded in Newton (N) and was used to calculate the push-out bond strength in mega-pascals (MPa) according to the following formula 18: ( ) The adhesion (bonding) surface area of each section was calculated as: (πr1 + πr2) *L. L was calculated as π = 3.14; r1 = coronal radius, in mm; r2 = apical radius, in mm; h = thickness of section in mm, L = adhesion area. ANOVA and Student t-test were performed as statistical analysis for push-out bond strength. RESULTS Mean values of push-out bond strength & standard deviations for all groups presented in (table 1). Both the highest and the lowest mean values for sealer push-out bond strength were seen at apical level of iRoot sp sealer without smear layer group C2 (4.889) and middle level of Apexit plus sealer without smear layer group A2 (1.125) respectively. The rest mean values for study groups were fluctuating between these values. To compare among the six groups systems at each level, ANOVA test was preformed to identify the presence of statistically significant differences for sealer push-out bond strength among different groups within each level. Highly significant differences were found in A1, B1, B2, and C2 at all levels while significant difference was shown in A2 while no significant difference was seen in C1 Table (2). The least significance difference test (LSD) was performed to evaluate the significant differences between six groups at each level and the results listed in Table (3) and showed the followings: - Highly significant differences between three levels in Group A1 (Apexit plus sealer with smear layer). J Bagh College Dentistry Vol. 25(4), December 2013 The effect of smear Restorative Dentistry 8 - Highly significant difference between coronal and middle level while no significant differences between coronal and apical, and between middle and apical levels in Group A2 (Apexit plus sealer without smear layer). - Significant differences between coronal and middle, and between middle and apical levels while highly significant differences between coronal and apical levels in Group B1 (AH plus sealer with smear layer). - No significant differences between coronal and middle, and between middle and apical levels while highly significant differences shown between coronal and apical levels in Group B2 (AH plus sealer without smear layer). - No significant differences between coronal and middle, and between middle and apical levels while significant differences appear between coronal and apical levels in Group C1 (iRoot sp sealer with smear layer). - Highly significant differences between coronal and middle and between coronal and apical levels while no significant differences between middle and apical levels in Group C2 (iRoot sp sealer without smear layer). Student t test showed: - No significant differences in push out bond strength at middle and apical levels in presence or absence smear layer of apexit plus sealer except at coronal level highly significant difference. - No significant differences in push out bond strength at all levels in presence or absence smear layer of AH plus sealer. - No significant differences in push out bond strength at coronal and apical levels in presence or absence smear layer of iRoot sp sealer except at middle level significant difference. DISCUSSION The adhesive strength of root canal sealers has been examined by various methods that include shear bond strength, microtensile bond strength, and pushout bond strength testing. The push-out test is easy to reproduce and interpret and provides a realistic assessment of bond strength to dentin even at low levels 13. Effect of Sealer Type on Bond Strength The result of present study showed the highest mean value of push –out bond strength in Group C2 that used iRoot sp sealer with removed smear layer (Table 1) and when compared with other sealers with same method of irrigation, there was very highly significant difference between group C2 iRoot sp sealer and group A2 Apexit plus sealer, significant difference between group C2 and group B2 AH plus sealer. These results are in agreement with the results of other studies 19,20, which conducted to evaluate and compare the fracture resistance of roots obturated with various contemporary canal-filling systems and it was concluded that the innovative bioceramic-based sealer (iRoot SP) may have the potentiality to strengthen endodontically treated teeth to a level comparable to that of intact teeth. This could be attributed to the nature of iRoot sp sealer being a true self adhesive material that would form a chemical and mechanical bond with dentin through the production of hydroxyapatite during setting when the material is exposed to a moist environment as that present within the dentinal tubules. In addition, the bioceramic sealer is hydrophilic, possessing a low-contact angle that would allow the sealer to spread easily over the canal wall providing adaptation and good hermetic seal through mechanical interlocking. In addition, the extremely fine particle size and the optimal premixed consistency introduced with a capillary tip introductory system might have enhanced its penetration to the full length of the canal. Furthermore, zirconium oxide, one of the constituents of the iRoot SP sealer, has been reported to possess high fracture toughness, tensile strength, and lower Young’s modulus 1,19,20. The result of this study disagreed with 14,22 who found that there was no significant difference between AH plus and Bioceramic sealer used with gutta percha, this may be related to difference in method of obturation, 22 in his study the canal was obturated by single cone technique in addition a slice thickness used in push out bond strength was 2mm while 14 measured only the push out bond strength of AH plus and Bioceramic sealer at middle third. In this study when the smear layer left at the apical area, group B1 AH Plus and group C1 iRoot sp showed a significantly higher values than group A1 Apexit plus, but there was no significant difference between B1 AH Plus and C1 iRoot sp groups, this coincide with the findings of 14,22-24 no significant difference between BC sealer and AH plus push out bond strength. The high bond strength of AH Plus may be explained by the formation of a covalent bond by an open epoxide ring to any exposed amino groups in collagen 25. Other investigations have shown a high-quality properties with epoxy resin–based sealers, including very low shrinkage while setting, long-term dimensional stability, flow, and long setting time, AH Plus sealer penetrates deeper into the surface micro- irregularities 26. J Bagh College Dentistry Vol. 25(4), December 2013 The effect of smear Restorative Dentistry 9 Effect of Root Section Level on Bond Strength The push-out bond strengths in the middle and apical specimens were significantly higher than those of the coronal specimens in group C1 iRoot sp sealer and highly significant in group C2. There were no significant differences between the push-out bond strengths in the middle and apical specimens in both groups C1 and C2. The result of this study is aligned with 24 who assessed the push-out bond strength of two new calcium silicate-based endodontic sealers in the root canals of extracted teeth iRoot sp and MTA Fillapex sealers. Three horizontal sections were prepared coronal, middle and apical. Their result showed that there were no significant differences between the bond strengths in the middle and apical slices. Some authors have reported the bond strengths of different sealer to dentin were higher in the apical one-third than in the coronal third 27-29. The higher bond strengths in the middle and apical specimens could be related to deeper sealer penetrations because of higher lateral condensation forces in apical third than coronal third and also could be a result of irregular dentine and devoid of tubules in apical part of roots which increase surface area of adhesion 24,30. Effect of Smear Layer Removal on Bond Strength This study showed that there were no significant differences in push out bond strength at coronal and apical levels in presence or absence of smear layer in iRoot sp sealer groups except at middle level a significant difference. This result agree with the result of other studies 14,31. The open tubules and the absence of smear layer do not improve adhesion of endodontic sealers. The authors suggest that perhaps the open tubules increase the stress at the sealer dentin interface and that the calcium and phosphate-rich smear layer and plugs are potential sites of sealer adhesion 31. Shokouhinejad et al. 14 compared the bond strength of a new bioceramic sealer (EndoSequence BC Sealer) and AH Plus in the presence or absence of smear layer, and they concluded that the presence or absence of smear layer did not affect the bond strength of EndoSequence BC Sealer. They explained that it may be due to Bioceramic sealer includes a similar composition to white MTA 1, and some studies revealed that removal of the smear layer caused significantly more microleakage in the root canals and root end cavities filled with MTA 5,32.So within the limitation of this in vitro study, can be concluded the presence or absence of smear layer did not significantly affect the bond strength of filling materials, the bond strengths of IRoot SP and AH Plus were significantly higher than those of Apexit plus, no significant difference between AH Plus and I Root SP groups in the presence of smear layer at the apical specimens and in terms of root segments, the bond strengths in the middle specimens and the apical specimens were higher compared with the bond strengths in the coronal specimens. REFERENCES 1. Zhang W, Li Z, Peng B. Assessment of a new root canal sealer’s apical sealing ability. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107: e79– 82. 2. Zhang W, Li Z, Peng B. Effect of iRoot SP on mineralization related genes expression in MG63 cells. J Endod 2010; 36:1978–82. 3. Sen BH, Piskin B, Baran N. The effect of tubular penetration of root canal sealers on dye microleakage. Int Endod J 1996; 29: 23–8. 4. Lalh MS, Titley K, Torneck CD, Friedman S. The shear bond strength of glass ionomer cement sealers to bovine dentine conditioned with common endodontic irrigants. Int Endod J 1999; 32: 430–5. (IVSL) 5. Yildirim T, Orucoglu H, Cobankara FK. Long-term evaluation of the influence of smear layer on the apical sealing ability of MTA. J Endod 2008; 34: 1537–40. 6. Drummond JL, Sakaquchi RL, Racean DC, Wozny J, Steinberg AD. Testing mode and surface treatment effects on dentin bonding. Journal of Biomedical Materials Research 1996; 32: 533–41. 7. Ureyen Kaya B, Keceli AD, Orhan H, Belli S. Micropushout bond strengths of gutta-percha versus thermoplastic synthetic polymer-based systems – an ex vivo study. Int Endod J 2007; 41: 211–8. 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Regional push-out bond strength and coronal microleakage of Resilon after different light-curing methods. J Endod 2007; 33: 1464-8. 19. Ghoneim AG, Lutfy RA, Sabet NE and Dalia M. Fayyad DM. Resistance to fracture of roots obturated with novel canal-filling systems. J Endod 2011; 37(11):1590-2. 20. Ulusoy Ö, Nayır Y, Darendeliler-Yaman S, Effect of different root canal sealers on fracture strength of simulated immature roots. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: 544-7. 21. Candeiro G, Correia F, Duarte M, Siqueira D. Evaluation of radiopacity, PH, Release of calcium ions, and flow of a bioceramic root canal sealer. J Endod 2012; 36(6): 842-5. 22. Naser SH. Push- out bond strength of different root canal obturation materials. A master thesis, Department of Conservative Dentistry, Al- Mustansiriyah University, 2012. 23. Ersahan S, Aydin C. Dislocation Resistance of iRoot SP, a Calcium Silicate– based Sealer, from Radicular Dentine. J Endod 2010; 36(12): 2000-2. 24. Sagsen B, Ustu¨ n Y, Demirbuga S, Pala K. Push-out bond strength of two new calcium silicate-based endodontic sealers to root canal dentine. Int Endod J 2011; 44(12): 1088-91.(IVSL) 25. Nunes VH, Silva RG, Alfredo E, Sousa MD, Sousa YT. Adhesion of Epiphany and AH Plus sealers to human root dentin treated with different solutions. Braz Dent J 2008; 19(1): 46-50. 26. Flores D, Rached-Júnior F, Versiani M1, Guedes D, Sousa-Neto M, Pécora J. Evaluation of physicochemical properties of four root canal sealers. Int Endod J 2011; 44(2): 126-35. (IVSL) 27. Gaston BA, West LA, Liewehr FR, Fernandes C, Pashley DH. Evaluation of regional bond strength of resin cement to endodontic surfaces. J Endod 2001; 27: 321– 4. 28. Mannocci F, Pilecki P, Bertelli E, Watson TF. Density of dentinal tubules affects the tensile strength of root dentin. Dent Mater 2004; 20: 293–6. 29. Muniz L, Mathias P. The influence of sodium hypochlorite and root canal sealers on post retention in different dentin regions. Oper Dent 2005; 30: 533–9. 30. Mjor IA, Smith MR, Ferrari M, Mannocci F. The structure of dentine in the apical region of human teeth. Int Endod J 2001; 34: 346–53. (IVSL) 31. Saleh IM, Ruyter IE, Haapasalo MP, Orstavik D. Adhesion of endodontic sealers: scanning electron microscopy and energy dispersive spectroscopy. J Endod 2003; 29(9): 595-601. 32. Yildirim T, Er K, Tas¸demir T, Tahan E, Buruk K, Serper A. Effect of smear layer and root-end cavity thickness on apical sealing ability of MTA as a root- end filling material: a bacterial leakage study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: e67–72. Table 1: Mean values of push-out bond strength and standard deviations at three levels in MPa. Group Level Mean ±SD A1 Coronal 2.8892 0.085815 Middle 1.2567 0.31708 Apical 1.734 0.406251 A2 Coronal 1.7935 0.49572 Middle 1.1251 0.607396 Apical 1.2975 0.548605 B1 Coronal 2.9464 0.521307 Middle 3.5701 0.717203 Apical 4.1697 0.629287 B2 Coronal 2.9971 0.836391 Middle 3.4908 0.707482 Apical 4.184 0.773615 C1 Coronal 3.4071 0.652522 Middle 3.8086 0.75308 Apical 4.2111 0.877046 C2 Coronal 3.5704 0.63928 Middle 4.488 0.526285 Apical 4.8891 0.598721 J Bagh College Dentistry Vol. 25(4), December 2013 The effect of smear Restorative Dentistry 11 Table 2: ANOVA test for push-out bond strength among three levels for each type of sealer Groups Areas Areas difference (d.f.=29) F-test P-value Sig. A1 Coronal 77.44 0.000 H.S. Middle Apical A2 Coronal 3.95 0.03 S. Middle Apical B1 Coronal 9.49 0.001 H.S. Middle Apical B2 Coronal 5.91 0.007 H.S. Middle Apical C1 Coronal 2.75 0.08 N.S. Middle Apical C2 Coronal 13.71 0.000 H.S. Middle Apical P ≥ 0.05: Non significant (NS) P < 0.05: Significant (S) P ≤ 0.01: Highly significant (HS) Table 3: Student t test of push out bond strength between two levels of each group of sealer P ≥ 0.05: Non significant (NS) P < 0.05: Significant (S) P ≤ 0.01: Highly significant (HS) Groups Levels t-test P-value Sig. A Coronal A1 6.89 0.000 H.S Coronal A2 Middle A1 0.61 0.55 N.S. Middle A2 Apical A1 2.02 0.06 N.S. Apical A2 B Coronal B1 -0.16 0.87 N.S. Coronal B2 Middle B1 0.29 0.78 N.S. Middle B2 Apical B1 -0.05 0.96 N.S. Apical B2 C Coronal C1 -0.58 0.57 N.S. Coronal C2 Middle C1 -2.34 0.03 S. Middle C2 Apical C1 -2.02 0.06 N.S. Apical C2