{Construction of a simple and selective electrochemical sensor based on Nafion/TiO2 for the voltammetric determination of olopatadine:} http://dx.doi.org/10.5599/jese.1117 91 J. Electrochem. Sci. Eng. 12(1) (2022) 91-103; http://dx.doi.org/10.5599/jese.1117 Open Access : : ISSN 1847-9286 www.jESE-online.org Original scientific paper Construction of a simple and selective electrochemical sensor based on Nafion/TiO2 for the voltammetric determination of olopatadine Mohammad Mehmandoust1,2, , Amirhossein Mehmandoust3 and Nevin Erk1,2, 1Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey 2Sakarya University, Biomaterials, Energy, Photocatalysis, Enzyme Technology, Nano & Advanced Materials, Additive Manufacturing, Environmental Applications, and Sustainability Research & Development Group (BIOENAMS R&D Group), 54187 Sakarya, Turkey 3Department of Chemistry, Faculty of Science, Urmia University, 1177, Urmia, Iran Corresponding authors: mehmandoust@ankara.edu.tr and erk@pharmacy.ankara.edu.tr Received: October 21, 2021; Accepted: October 14, 2021; Published: November 20, 2021 Abstract A selective and facile voltammetric method based on titanium dioxide nanoparticles and Nafion (Nafion/TiO2 NPs) on the screen-printed electrode (SPE) was proposed for olopatadine determination. Followed by the synthesis of TiO2 nanoparticles, various methods, including high-resolution transmission electron microscopy (HR-TEM), ultraviolet-visible spectroscopy (UV-Vis), energy-dispersive X-ray (EDX) Raman spectrum, and electrochemical impedance spectroscopy (EIS) were utilized to characterize the nanomaterials. Nafion/TiO2 on the screen-printed electrode (NFN/TiO2/SPE) was used to determine olopatadine in concentration ranges of 0.01 to 0.07 and 0.07 to 14.6 µM with a limit of quantification as low as 7.0 nM, via differential pulse voltammetry technique. The NFN/TiO2/SPE offered a high-performance ability to determine olopatadine in the eye drop sample with satisfactory recovery data of 98.2–99.0 %. Also, the developed electrode showed good reproducibility, repeatability, and high selectivity features. The obtained results indicate that NFN/TiO2/SPE could be utilized as an appropriate candidate for electrochemical olopatadine sensing. Keywords Monitoring; screen-printed electrode, differential pulse voltammetry. Introduction Olopatadine (OLP) is a tricyclic drug that impedes mast cell mediator liberating and possesses histamine H1-receptor antagonist activity. Olopatadine hydrochloride is utilized to treat allergic http://dx.doi.org/10.5599/jese.1117 http://dx.doi.org/10.5599/jese.1117 http://www.jese-online.org/ mailto:mehmandoust@ankara.edu.tr mailto:erk@pharmacy.ankara.edu.tr J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 NAFION/TiO2 SENSOR FOR DETERMINATION OF OLOPATADINE 92 conjunctivitis. In vivo and in vitro, a relatively specific histamine H1 antagonist reduces type 1 imme- diate hypersensitivity, including histamine-induced effects on human conjunctival epithelial cells [1]. The physiologic effect of olopatadine is utilizing decreased histamine release. The IUPAC name of olopatadine is 2-[(11Z)-11-[3-(dimethylamino) propylidene]-6H-benzo[c][1]benzoxepin-2-yl]acetic acid and it is shown in Scheme. 1. Scheme. 1. The chemical structure of olopatadine Currently, different analytical methods have been proposed to determine olopatadine, including UV–Vis spectrophotometry [2-4] and high-performance liquid chromatography (HPLC) [5, 6]. However, it is worth noting that the current electrode systems, such as screen-printed electrodes (SPE), present significant advantages such as simple operation and portability, compared to the widespread electrode systems such as glassy carbon electrodes [7]. Therefore, it is desirable to fabricate a portable electrochemical sensor to determine antihistamine agents in real samples. In this regard, fabricating an electrode with a novel nanomaterial is essential for electrochemical applications engineering a multi-functional sensing material[8-16]. Nanomaterials showed many advantages in different branches of science [17-24]. In recent years, nanostructured materials, including metal oxides nanoparticles, have been used to fabricate the modified electrodes for biological, pharmaceutical, energy and environmental purposes [25-33]. Among the various nanomaterials, titanium dioxide (TiO2) has outstanding properties such as appropriate sensitivity and stability for its strong affinity to a phosphate group, photocatalytic effect, porosity, steadiness, and high specific surface areas [34,35]. TiO2 nanoparticles have also been applied in wide potential windows, improving the electrode's stability, increasing the electrode response's repeatability [36,37]. Combining metallic characteristics' electronic conductivity and electroactivity with Nafion's (NFN) high ionic conductivity and ion-exchange capacity opens up many possibilities for nanoparticle design in NFN modified electrodes with unique features and uses [38]. This study employed the SPE electrodes and the promising development of a susceptible electrochemical sensor based on titanium dioxide nanoparticles and Nafion. It would be expected that the combination of TiO2 with Nafion could provide excellent electrochemical sensing performance for olopatadine. Titanium dioxide nanoparticles were used to prepare additional improvements because their stated physicochemical features include strong biocompatibility, solid adsorptive ability, large surface area, thermal stability, non-toxicity, and electrical/electrochemical capabilities. The synergic effect of Nafion and TiO2 remarkably enhanced the electrocatalytic activity towards the OLP and consequently allowed us to reach its detection limit up to 7.0 nM. Moreover, NFN/TiO2/SPE exhibited a promising analytical performance in the wide linear concentration ranges of 0.01 to 0.07 and 0.07 to 14.66 µM towards the OLP under optimized experimental conditions. The developed electrochemical sensing platform was successfully utilized to determine the amount of OLP in real samples with satisfactory recovery. M. Mehmandoust et al. J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 http://dx.doi.org/10.5599/jese.1117 93 Experimental Chemical and reagents All materials were of analytical purity and have not been subjected to a further purification process. C3H6O (acetone), C2H5OH (ethanol), NaOH (sodium hydroxide) were supplied from Merck (Darmstadt, Germany). L-ascorbic acid (99.0 %), L-cysteine (97.0 %), L-arginine (98.0 %), dopamine hydrochloride (99.0 %), glucose (99.5 %), uric acid (99.0 %), potassium hexaferrocyanide(II) (K4Fe(CN)6) (99.5 %), potassium hexacyanoferrate(III) (K3Fe(CN)6) (99.5 %) were obtained from Sigma-Aldrich (Germany). Olopatadine and eye drop (Patanol®) were acquired from NOVARTIS Turkey Inc. Deionized (DI) water was utilized in the experiments. The stock solution of 1.0 mM OLP was prepared via DI water. Apparatus Energy-dispersive X-ray analysis of the sample was conducted using a Quanta FEG 450 field emission scanning electron microscope. The sample's morphology was characterized by transmission electron microscopy (FEI TALOS F200S) operated at 200 kV. Raman spectrum of TiO2 NPs was observed from Renishaw inVia with a 785 nm solid-state diode laser at 1.0 mW power and 10 s acquisition times. The diffused reflectance (DRS) analysis was conducted by a Shimadzu UV-2401 UV–Vis spectrophotometer. Synthesis of TiO2 nanoparticles TiO2 nanoparticles were synthesized by the microwave-assisted hydrothermal method (MWHT). As comparing the conventional hydrothermal method (HT), the MWHT also has several benefits as follows; i) shorter reaction time and ii) complete reaction at lower temperatures. Thus, the microwave reaction formation helps shorten the reaction time and form reactions at lower temperatures. The microwave process may also facilitate TiO2 crystals to be developed, and microwave radiation can accelerate the TiO2 nucleation. Moreover, low temperatures and power for microwave-assisted hydrothermal preparation may observe many morphological structures mentioned above. The Teflon- covered autoclave microwave-assisted hydrothermal vessel received 5.0 mL titanium isopropoxide, 5.0 mL 1.0 M NaOH, and 60.0 mL ultrapure water. A CEM Mars 5 microwave digestion machine was employed to prepare the reaction solution, forcefully agitated at room temperature for 30 minutes. Microwave-assisted hydrothermal synthesis was performed at 100 oC for 30 minutes with a micro- wave output of 380 W. White TiO2 NPs were washed with ultrapure water and ethanol after centri- fugation at 5000 rpm for 15 minutes. Subsequently, the powder was dried overnight at 70 oC [39]. Fabrication of NFN/TiO2/SPE Firstly, The as-synthesized TiO2 nanoparticles (0.5 mg) and 1.0 % Nafion were homogeneously dispersed in 1.0 ml Milli-Q water by ultrasonication until a uniform TiO2 dispersion was gained. Following this, a 10.0 µL of NFN/TiO2 dispersion was coated onto the previously cleaned SPE surface by drop-casting method and dried at room temperature. NFN/TiO2/SPE was mildly rinsed with Milli-Q water to remove the non-attached composite materials on the surface of SPE. Moreover, to compare the electrochemical performance of the prepared composite materials, the SPE surfaces were also modified with TiO2 as precursor material. Results and discussion Characterization of TiO2 FE-SEM, HR-TEM, and Raman spectroscopy techniques were implemented to characterize TiO2 nanoparticles synthesized via the microwave hydrothermal method, and the obtained results were http://dx.doi.org/10.5599/jese.1117 J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 NAFION/TiO2 SENSOR FOR DETERMINATION OF OLOPATADINE 94 exhibited in Fig. 1. In Fig. 1, the diffused reflectance spectrum of TiO2 NPs was depicted. TiO2 NPs' DRS spectra reflected a 380-800 nm light spectrum. At a wavelength of 383 nm, TiO2 NPs exhibited a DRS signal. A transfer of electrons from the O 2p orbital to the Ti 3d orbital was presented by this high peak [40]. A Kubelka-Munk function was used to determine the bandgap values of this sample, and the Kubelka-Munk graph of produced TiO2 NPs was illustrated in Fig 1. inset. TiO2 NPs were of an estimated bandgap of 3.14 eV. Anatase TiO2 has a theoretical bandgap of 3.2 eV [41]. Figure 1. DRS and hν / eV of synthesized TiO2 NPs The TEM images of TiO2 NPs were depicted in Fig. 2a and 2b. Aggregates with a nanoparticle diameter of 15 nm were detected in the TiO2 sample. The d-spacing of the (101) and (110) planes of anatase structure corresponded to the lattice spacing of 0.318 and 0.325 nm, respectively. a b c d Fig. 2. TEM images (a, b), Raman spectra (c), and EDX spectra (d) of TiO2 (F R h  )2 / ( e V / m )2 M. Mehmandoust et al. J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 http://dx.doi.org/10.5599/jese.1117 95 The Raman spectra of TiO2 NPs are illustrated in Fig. 2c. The spectrum presented the five distinctive peaks of anatase TiO2. The following peaks appeared: Eg = 145, 197, 394, 518, and 639 cm−1 [42]. The crystalline size of the material was likewise impacted by the Raman peak broadenings. The crystalline size of the material might be estimated using the Raman low frequency Eg mode peaks (143, 155 cm-1). The TiO2 NPs had a full-width hall maximum of 16 cm-1. According to the Raman spectra, these predicted crystalline size estimates were likewise in excellent agreement with those computed according to Debye-Scherrer. The existence of elements was verified by energy-dispersive X-ray spectroscopy (Fig. 2d). Electrochemical behavior of Olopatadine at NFN/TiO2/SPE The electrochemical performance of the modified sensing platforms was assessed by cyclic voltammetry (CV) in the presence of 0.1 M Britton-Robinson (B-R) buffer at pH 4.0 containing 100.0 µM OLP at a potential scan rate of 50.0 mV s-1. As can be seen in Fig. 3, a well-defined oxidation signal of OLP at 1.0V was achieved at bare SPE (blue line) and modified SPE surfaces with TiO2 (yellow line) and NFN/TiO2 (red line). The corresponding voltammetric curves represented by CV demonstrated that the modification of SPE surfaces by NFN/TiO2 remarkably enhanced the sensitivity towards the OLP with an excellent electrocatalytic activity compared to that of bare SPE due to their larger surface areas. The corresponding oxidation current for OLP at NFN/TiO2/SPE was 2.7 µA, which was 2.2 times greater than that of unmodified SPE. This significant enhancement in the voltammetric response of OLP with a negative oxidation potential shift that occurred at NFN/TiO2/SPE could be assigned to the synergistic effect of NFN and metal oxides. Fig. 3. CVs of NFN/TiO2/SPE (blue line), TiO2/SPE (red line), and bare SPE (black line) electrode in pH 4.0 BR buffer ata scan rate of 50.0 mV s-1. The ability of electron transfer rate of the NFN/TiO2/SPE electrochemical sensors was evaluated by EIS using [Fe(CN)6]3−/4− solution as a redox probe. They were obtained at different electrodes of the bare electrode, TiO2/SPE, and NFN/TiO2/SPE in the presence of 5.0 mM [Fe(CN)6]3−/4− in 0.1 M KCl. By comparing different electrodes, the charge transfer resistance (Rct) values were calculated to be 7.4, 5.8 and 2.2 kΩ for bare electrode, TiO2/SPE, and NFN/TiO2/SPE, respectively (Fig. 4). The results confirmed that the synergistic effect of TiO2 and NFN in the modified electrode improved due to their properties, such as the increase in the conductivity of the electrode and the electron transfer. According to Fig. 4, the electrochemical reaction occurred more efficiently on NFN/TiO2/SPE thanks to the least charge transfer resistance and the special electron transfer rate [32]. http://dx.doi.org/10.5599/jese.1117 J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 NAFION/TiO2 SENSOR FOR DETERMINATION OF OLOPATADINE 96 Fig. 4. EIS responses at the bare electrode, TiO2/SPE, and NFN/TiO2/SPE in [Fe(CN)6]3−/4− as redox probe containing 0.1 M KCl Optimization of experimental conditions Investigation of pH and scan rate The impact of pH upon the voltammetric response of OLP was investigated at NFN/TiO2/SPE using a B-R buffer system in the varying pH range from 2.0 to 7.0. As depicted in Fig. 5a, the oxidation peak current of OLP increased gradually from 2.0 to 4.0 and then attained its maximum current value with a good peak shape at pH 4.0. a b c d Fig. 5. (a) Impact of varying pH values on the peak current of OLP; (b) CVs of 100.0 µM OLP on NFN/TiO2/SPE in pH 4.0 B-R buffer at varying scan rates (10.0, 25.0, 50.0,100.0, 150.0, 200.0, 300.0, 400.0 and 500.0 mVs-1); (c) The plot of the logIpa vs. log v obtained at the surface of NFN/TiO2/SPE; (d) The plot of Epa vs. natural logarithm of scan rate for OLP M. Mehmandoust et al. J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 http://dx.doi.org/10.5599/jese.1117 97 Above pH >4.0, the corresponding peak current of OLP started to decrease continually until pH 7.0. Therefore, the best pH condition for OLP was selected as pH 4.0 for further analytical measure- ments. The linear curve equation that plotted the potentials against pHs was Ep =−0.0381pH+ 1.1405 (R2 = 0.993). The slope attained for the principal oxidation peak in the first scan was 38.1 mV/pH, close to the theoretical value of 59 mV/pH. These results demonstrated that the transferred electron and proton numbers were equal[43]. The scan rate step is an essential parameter for determining the electrode reaction of the analyte at the working electrode. Fig. 5b exhibited the scan rate effect on the voltammetric response of OLP at NFN/TiO2/SPE in 0.1 M B-R buffer (pH 4.0) at different scan rates ranging from 10.0 to 500.0 mV s-1.The plot of the logarithm of the oxidation peak current (log Ipa ) against the logarithm of the scan rate (log v) was found to be linear with the following regression equation: log Ipa = 0.6171 log v –0.7381 (R2=0.9913), indicating a diffusion-controlled electrode reaction with a contribution of the adsorption (Fig. 5c). Likewise, it exhibited that the oxidation peak potential (Epa) of OLP shifted towards the positive direction with the scan rate's enhancement, which recommended the expected result for an irrever- sible electrode reaction. The number of electrons transferred in the oxidation step of OLP can be calculated by Laviron's equation from the slope of Epa vs. ln v plot. The relationship between Epa vs. ln v was expressed as: Epa=0.0398 ln v + 0.8727 with a correlation coefficient of 0.9921. The number of electrons in the electro-oxidation of OLP was estimated to be ~1.25, demonstrating that the oxidetion reaction of OLP at NFN/TiO2/SPE is of a single electron and proton transfer process. As a result, using pH scan and scan rate studies, a possible oxidation mechanism for OLP was illustrated in Scheme.2. The amount of OLP absorbed on the surface of the NFN/TiO2/SPE electrode was observed by the following equation: 2 2 p 4 4 nFQv n F v I RT RT  = = (1) Where n presents the number of electrons transferred, F is the Faraday's constant (96485 C mol-1), A represents the area of the electrode,  is the surface coverage of adsorbed substance, Q exhibits the quantity of charge consumed, and v is scan rate. Using the relationship of Ip with ʋ and integrating the peak area for OLP oxidation, the values of τwere calculated to be 20 µmol cm-2. Scheme.2 Possible electro-oxidation mechanism of OLP at NFN/TiO2/SPE surface Effect of accumulation potential and time Accumulation time and potential are essential aspects that significantly impact sensitivity in analyses. For an adsorption-controlled electrode reaction, optimizing pre-concentration steps of the target analyte on the electrode surface is an essential factor in enhancing the sensitivity of the proposed electroanalytical method. For this purpose, the effect of accumulation potential (Eacc) and http://dx.doi.org/10.5599/jese.1117 J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 NAFION/TiO2 SENSOR FOR DETERMINATION OF OLOPATADINE 98 time (tacc) on the oxidation responses of OLP was investigated by DPV at NFN/TiO2/SPE. For Eacc optimization, the oxidation peak current of OLP was measured by applying various Eacc in the range of 0.2 to 0.9 V under a constant tacc of 30 s (Fig. 6a). The maximum peak current for OLP was achieved at the Eacc of 0.50 V. In addition, the oxidation peak current of OLP at NFN/TiO2/SPE enhanced piecemeal with increasing of tacc from 15 to 60 s under fixed Eacc of 0.50 V and reached its maximum value at tacc of 60 s (Fig. 6b). For longer tacc values, the peak currents for OLP have almost remained stable due to the saturation of NFN/TiO2/SPE with target molecules. Hence, Eacc value of 0.50 V and tacc value of 60.0 s were determined as the optimal accumulation step parameters for further analytical measurements. a b Fig. 6. (a) Effect of tacc upon the peak current with 0.5 V of Eacc, (b) Effect of Eacc upon the peak current with 60 s of tacc for OLP in B-R (pH 4.0) on NFN/TiO2/SPE Analytical performance Differential pulse voltammetry (DPV) for OLP was implemented to enhance the sensitivity of the proposed electrochemical sensing platform. DPV investigated the analytical performance of developed NFN/TiO2/SPE towards the different concentrations of OLP under optimized experimental conditions (such as pH of the supporting electrolyte, scan rate, accumulation potential, and time). Fig. 7 illustrated the DP voltammograms and the corresponding calibration curve by plotting the oxidation peak current against the increasing OLP concentration (COLP). Two linear calibration ranges from 0.01 to 0.07 and 0.07 to 14.66 µM for OLP were obtained at the surface of NFN/TiO2/SPE with the following regression equations: Ipa = 3.101 COLP + 0.255 (R2=0.9924) and Ipa = 0.211COLP + 0.5597 (R² = 0.9982). The sensitivity (slope) of the second linear segment for OLP decreased due to the kinetic limitations. The limit of detection (LOD) value for OLP was calculated as 7.0 nM (3.3Sb/m = 3) [24]. Here, Sb represents the standard deviation of the peak current (n = 10) of 0.01 µM olopatadine, and m is the slope of calibration plots. This improved sensing performance of NFN/TiO2/SPE for OLP could be attributed to the synergic effect of NFN and metal oxide nanoparticles, which provided an enhanced conductivity, fast electron transfer, and large surface area. The analytical performance of the proposed sensing platform was compared with the similar developed electrochemical sensing platforms for the determination of OLP. The obtained analytical parameters were compared to similar reported analytical methods, which have been utilized to sense OLP (Table 1). The analytical performance of NFN/TiO2/SPE, which was of wide linearity and low LOD value, exhibited to be much more appropriate than other comparative analytical methods towards the detection of OLP. The results suggested that NFN/TiO2/SPE was of comparable analytical performance (linearity and LOD) towards the OLP (Table 2). M. Mehmandoust et al. J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 http://dx.doi.org/10.5599/jese.1117 99 Table. 1. A collation between diverse methods to detect OLP by the developed methods Method Materials Linear range, µM LOD, µM Ref. Potentiometric OLP–PM-CPE 3×10-5 - ×10-2 1.39×10-5 [44] Potentiometric Poly/ONOE 10-5 - 10-2 5.0×10-6 [45] CV HMDE 10-8 - 4.0×10-7 5.7×10-9 [46] DPV NFN/TiO2/SPE 10-8 - 14.66×10-6 7.01×10-8 This work a b Fig. 7. (a) DPVs of NFN/TiO2/SPE in 0.1 M BR buffer at pH 4.0 containing different concentrations of OLP, (b) plots of Ip vs. OLP concentrations Table. 2. The analytical parameters were obtained by electrochemical determination of OLP at NFN/TiO2/SPE in 0.1 M B-R(pH 4.0) Parameters NFN/TiO2/SPE Measured potential, mV 500 Measured time, s 60 Linear working range, µM 0.01-14.63 Slope, µA/µM 0.2188 R2 0.9914 Intercept 0.4829 LOD, nM 10 LOQ, nM 33.4 Interference test study The detection capability of the proposed sensing platform towards the OLP in the presence of various potential interfering agents, which have commonly been found in biological samples, was as- sessed by DPV under optimized experimental conditions. The results exhibited that a 100-fold excess of biological compounds (ascorbic acid, dopamine, glucose, and uric acid) and amino acids (l-cysteine and l-arginine) did not show no or negligible interference effect in the determination of OLP (Table 3). Table 3. Influence of various interfering agents on OLP (1.0 µM) at NFN/TiO2/SPE (n = 4), CInterf agents : COLP = 100 : 1 Interfering agents RSD, % Ascorbic acid 1.16 Dopamine 0.32 Glucose 0.52 Uric acid 0.91 l-cysteine 1.3 l-arginine 2.41 http://dx.doi.org/10.5599/jese.1117 J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 NAFION/TiO2 SENSOR FOR DETERMINATION OF OLOPATADINE 100 The corresponding relative errors for OLP were lower than ±2 %, which was correlated with the tolerance limit defined in the selectivity measurements, indicating that NFN/TiO2/SPE has a promising selectivity for the determination of OLP. Repeatability, reproducibility To prove the repeatability of the developed electrochemical sensor, the five replicate DPV measurements for OLP were conducted using the same NFN/TiO2/SPE in pH 4.0 B-R buffer containing 1.0 µM OLP under optimized accumulation conditions. Similarly, the NFN/TiO2/SPE reproducibility was evaluated by monitoring the 1.0 OLP solution in pH 4.0 B-R using five independently prepared electrochemical sensors. The corresponding relative standard deviation (RSD) values for repeatability and reproducibility tests were found as 0.73 and 2.50 %, respectively, confirming that NFN/TiO2/SPE has satisfactory repeatability and reproducibility towards the detection of OLP. Real sample analysis In real sample analysis, the eye drop sample with a spiked value of olopatadine was employed for the NFN/TiO2/SPE capability. The obtained data by the standard addition method were tabulated in Table 4. Good recovery data 98.2 and 99.0 % confirmed the powerful ability of NFN/TiO2/SPE in determining olopatadine in the real samples. Table 4. The results data relative to analysis of olopatadine in the real sample. Sample C / µM RSD, % Recovery, % Added Found Eye drop 2.0 1.98 3.49 99.0 4.0 3.93 4.21 98.2 Conclusions In this paper, a new method for fabricating a selective and straightforward electrochemical sensor based on NFN/TiO2 was proposed for future electrochemical sensing applications to determine the trace level of OLP in the eye drop sample. The modification of NFN/TiO2 on SPE dramatically enhanced the electrocatalytic activity towards OLP oxidation due to its large surface area, improved electron transfer kinetics, and high adsorption ability. NFN/TiO2/SPE illustrated a highly desirable analytical performance at the concentration ranges of 0.01 to 0.07 and 0.07 to 14.63 µM with a meager detection limit of 7.0 nM. The developed portable sensing platform also presents several advantages, such as high reproducibility, repeatability, and appropriate selectivity with an RSD of less than 5 %. The feasibility of the proposed sensing platform was successfully tested in an eye drop sample with adequate accuracy, precision results, and recovery of 98.2-99.0 %. It can be speculated that the proposed novel NFN/TiO2/SPE-based electrochemical sensing platform could be utilized as an alternative analytical approach with a high potential to determine antiviral agents such as OLP in future clinical applications. Acknowledgments: This work was supported by the Scientific Research Projects Commission of Ankara University (Project Number: 21B0237005 and 19L0237004). M. Mehmandoust et al. J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 http://dx.doi.org/10.5599/jese.1117 101 References [1] K. Ohmori, K. Hasegawa, T. Tamura, K. Miyake, M. Matsubara, S. Masaki, A. Karasawa, N. Urayama, K. Horikoshi, J. Kajita, Arzneimittelforschung 54 (2004) 12, 809-829. https://doi.org/10.1055/s-0031-1297036 [2] S. Dey, Y. Reddy, B. Swetha, S. Kumar, P. Murthy, S. Sahoo, D. Kumar, S. Patro, S. Mohapatra, International Journal of Pharmacy and Pharmaceutical Sciences 2(4) (2010) 212-218. http://www.ijppsjournal.com/Vol2Suppl4/812.pdf [3] D. Jain, P. Basniwal, Journal of Pharmaceutical Research 12(2) (2013) 48-52. https://www.researchgate.net/publication/253240922_Spectrophotometric_Determination _of_Olopatadine_Hydrochloride_in_Eye_Drops_and_Tablets [4] M. M. Annapurna, G. H. Bindu, I. Divya, Drug Invention Today 4 (2012) 8. [5] S. K. Raul, B. Kumar, A. K. Patnaik, N. N. Rao, Asian Journal of Research in Chemistry 5(11) (2012) 1395-1398. https://ajrconline.org/AbstractView.aspx?PID=2012-5-11-16 [6] P. K. Basniwal, D. Jain, Journal of Analytical Science and Technology 4(1) (2013) 12. https://doi.org/10.1186/2093-3371-4-12 [7] S. Tajik, Y. Orooji, Z. Ghazanfari, F. Karimi, H. Beitollahi, R.S. Varma, H.W. Jang, M. Shokouhimehr, Journal of Food Measurement and Characterization 15 (2021) 3837-3852. https://doi.org/10.1007/s11694-021-00955-1 [8] E. Mirmomtaz, A. Asghar Ensafi, H. Karimi‐Maleh, Electroanalysis 20(18) (2008) 1973-1979. https://doi.org/10.1002/elan.200804273 [9] A. A. Ensafi, H. Karimi‐Maleh, Drug testing and analysis 3(5) (2011) 325-330. https://doi.org/10.1002/dta.232 [10] [A. A. Ensafi, H. Karimi‐Maleh, S. Mallakpour, Electroanalysis 23(6) (2011) 1478-1487. https://doi.org/10.1002/elan.201000741 [11] H. Karimi-Maleh, M. Keyvanfard, K. Alizad, M. Fouladgar, H. Beitollahi, A. Mokhtari, F. Gholami-Orimi, International Journal of Electrochemcal Science 6(12) (2011) 6141-6150. http://www.electrochemsci.org/papers/vol6/6126141.pdf [12] J. Raoof, R. Ojani, H. Karimi-Maleh, Journal of Applied Electrochemistry 39(8) (2009) 1169- 1175. https://doi.org/10.1007/s10800-009-9781-x [13] M. A. Khalilzadeh, H. Karimi-Maleh, A. Amiri, F. Gholami, Chinese Chemical Letters 21(12) (2010) 1467-1470. https://doi.org/10.1016/j.cclet.2010.06.020 [14] S. Tajik, Y. Orooji, F. Karimi, Z. Ghazanfari, H. Beitollahi, M. Shokouhimehr, R. S. Varma, H. W. Jang, Journal of Food Measurement and Characterization 15 (2021) 4617-4622. https://doi.org/10.1007/s11694-021-01027-0 [15] Y. Orooji, P. N. Asrami, H. Beitollahi, S. Tajik, M. Alizadeh, S. Salmanpour, M. Baghayeri, J. Rouhi, A. L. Sanati, F. Karimi, Journal of Food Measurement and Characterization 15 (2021) 4098-4104. https://doi.org/10.1007/s11694-021-00982-y [16] M. Alizadeh, P.A. Azar, S.A. Mozaffari, H. Karimi-Maleh, A.-M. Tamaddon, Frontiers in Chemistry 8 (2020) 814. https://doi.org/10.3389/fchem.2020.00814 [17] M. Al Sharabati, R. Abokwiek, A. Al-Othman, M. Tawalbeh, C. Karaman, Y. Orooji, F. Karimi, Environmental Research 202 (2021) 111694. https://doi.org/10.1016/j.envres.2021.111694 [18] F. Karimi, A. Ayati, B. Tanhaei, A. L. Sanati, S. Afshar, A. Kardan, Z. Dabirifar, C. Karaman, Environmental Research 203 (2021) 111753. https://doi.org/10.1016/j.envres.2021.111753 [19] A. Aykan, O. Karaman, C. Karaman, N. Atar, M. L. Yola, Surfaces and Interfaces 25 (2021) 101293. https://doi.org/10.1016/j.surfin.2021.101293 [20] C. Karaman, E. Bayram, O. Karaman, Z. Aktaş, Journal of Electroanalytical Chemistry 868 (2020) 114197. https://doi.org/10.1016/j.jelechem.2020.114197 [21] O. Karaman, H. Özdogan, Y.A. Üncü, C. Karaman, A.G. Tanır, Kerntechnik 85(5) (2020) 401- 407. https://doi.org/10.3139/124.200022 http://dx.doi.org/10.5599/jese.1117 https://doi.org/10.1055/s-0031-1297036 http://www.ijppsjournal.com/Vol2Suppl4/812.pdf https://www.researchgate.net/publication/253240922_Spectrophotometric_Determination_of_Olopatadine_Hydrochloride_in_Eye_Drops_and_Tablets https://www.researchgate.net/publication/253240922_Spectrophotometric_Determination_of_Olopatadine_Hydrochloride_in_Eye_Drops_and_Tablets https://ajrconline.org/AbstractView.aspx?PID=2012-5-11-16 https://doi.org/10.1186/2093-3371-4-12 https://doi.org/10.1007/s11694-021-00955-1 https://doi.org/10.1002/elan.200804273 https://doi.org/10.1002/dta.232 https://doi.org/10.1002/elan.201000741 http://www.electrochemsci.org/papers/vol6/6126141.pdf https://doi.org/10.1007/s10800-009-9781-x https://doi.org/10.1016/j.cclet.2010.06.020 https://doi.org/10.1007/s11694-021-01027-0 https://doi.org/10.1007/s11694-021-00982-y https://doi.org/10.3389/fchem.2020.00814 https://doi.org/10.1016/j.envres.2021.111694 https://doi.org/10.1016/j.envres.2021.111753 https://doi.org/10.1016/j.surfin.2021.101293 https://doi.org/10.1016/j.jelechem.2020.114197 https://doi.org/10.3139/124.200022 J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 NAFION/TiO2 SENSOR FOR DETERMINATION OF OLOPATADINE 102 [22] H. Karimi-Maleh, A. Ayati, R. Davoodi, B. Tanhaei, F. Karimi, S. Malekmohammadi, Y. Orooji, L. Fu, M. Sillanpää, Journal of Cleaner Production 291 (2021) 125880. https://doi.org/ 10.1016/j.jclepro.2021.125880 [23] E. Vatandost, A. Ghorbani-HasanSaraei, F. Chekin, S. N. Raeisi, S.-A. Shahidi, Food Chemistry: X 6 (2020) 100085. https://doi.org/10.1016/j.fochx.2020.100085 [24] M. Mehmandoust, N. Erk, C. Karaman, F. Karimi, S. Salmanpour, Micromachines 12(11) (2021) 1334. https://doi.org/10.3390/mi12111334 [25] H. Karimi-Maleh, F. Karimi, L. Fu, A. L. Sanati, M. Alizadeh, C. Karaman, Y. Orooji, Journal of Hazardous Materials 423 (2021) 127058. https://doi.org/10.1016/j.jhazmat.2021.127058 [26] C. Karaman, Topics in Catalysis (2021). https://doi.org/10.1007/s11244-021-01500-6 [27] C. Karaman, O. Karaman, N. Atar, M. L. Yola, SPhysical Chemistry Chemical Physics 23(22) (2021) 12807-12821. https://doi.org/10.1039/D1CP01726H [28] C. Karaman, Z. Aktaş, E. Bayram, O. Karaman, Ç. Kızıl, ECS Journal of Solid State Science and Technology 9(7) (2020) 071003. https://doi.org/10.1149/2162-8777/abb192 [29] C. Karaman, O. Karaman, B. B. Yola, İ. Ulker, N. Atar, M. L. Yola, New Journal of Chemistry 45 (2021) 11222-11233. https://doi.org/10.1039/D1NJ02293H [30] H. Medetalibeyoğlu, M. Beytur, S. Manap, C. Karaman, F. Kardaş, O. Akyıldırım, G. Kotan, H. Yüksek, N. Atar, M.L. Yola, ECS Journal of Solid State Science and Technology 9(10) (2020) 101006. https://doi.org/10.1149/2162-8777/abbe6a [31] N. H. Khand, A. R. Solangi, S. Ameen, A. Fatima, J .A. Buledi, A. Mallah, S. Q. Memon, F. Sen, F. Karimi, Y. Orooji, Journal of Food Measurement and Characterization 15 (2021) 3720-3730. https://doi.org/10.1007/s11694-021-00956-0 [32] M. Alizadeh, M. Mehmandoust, O. Nodrat, S. Salmanpour, N. Erk, Journal of Food Measure- ment and Characterization 15 (2021) 5622-5629. https://doi.org/10.1007/s11694-021- 01128-w [33] H. Karimi-Maleh, Y. Orooji, F. Karimi, M. Alizadeh, M. Baghayeri, J. Rouhi, S. Tajik, H. Beitollahi, S. Agarwal, V. K. Gupta, Biosensors and Bioelectronics 184 (2021) 113252. https://doi.org/10.1016/j.bios.2021.113252 [34] L. Hu, C.-C. Fong, X. Zhang, L. L. Chan, P.K. Lam, P. K. Chu, K.-Y. Wong, M. Yang, Environmental Science and Technology 50(8) (2016) 4430-4438. https://doi.org/10.1021/acs.est.5b05857 [35] M. Khodari, E. Rabie, H. Assaf, International Journal of Science and Research 5(11) (2015) 1501-1505. https://www.ijsr.net/get_abstract.php?paper_id=ART20163056 [36] A. Mao, H. Li, Z. Cai, X. Hu, Journal of Electroanalytical Chemistry 751 (2015) 23-29. https://doi.org/10.1016/j.jelechem.2015.04.034 [37] A. A. Ensafi, E. Khoddami, B. Rezaei, H. Karimi-Maleh, Colloids and Surfaces B: Biointerfaces 81(1) (2010) 42-49. https://doi.org/10.1016/j.colsurfb.2010.06.020 [38] S. Cheraghi, M. A. Taher, H. Karimi-Maleh, F. Karimi, M. Shabani-Nooshabadi, M. Alizadeh, A. Al-Othman, N. Erk, P. K. Y. Raman, C. Karaman, Chemosphere 287(2) (2021), 132187. https://doi.org/10.1016/j.chemosphere.2021.132187 [39] M. Mehmandoust, S. Çakar, M. Özacar, S. Salmanpour, N. Erk, Topics in Catalysis (2021). https://doi.org/10.1007/s11244-021-01479-0 [40] J.-H. Huang, P. Y. Hung, S.-F. Hu, R.-S. Liu, Journal of Materials Chemistry 20(31) (2010) 6505- 6511. http://doi.org/10.1039/C0JM00549E [41] B. Roose, S. Pathak, U. Steiner, Chemical Society Reviews 44(22) (2015) 8326-8349. https://doi.org/10.1039/C5CS00352K [42] T. K. Das, P. Ilaiyaraja, P. S. Mocherla, G. Bhalerao, C. Sudakar, Solar Energy Materials and Solar Cells 144 (2016) 194-209. https://doi.org/10.1016/j.solmat.2015.08.036 https://doi.org/10.1016/j.fochx.2020.100085 https://doi.org/10.3390/mi12111334 https://doi.org/10.1016/j.jhazmat.2021.127058 https://doi.org/10.1007/s11244-021-01500-6 https://doi.org/10.1039/D1CP01726H https://doi.org/10.1149/2162-8777/abb192 https://doi.org/10.1039/D1NJ02293H https://doi.org/10.1149/2162-8777/abbe6a https://doi.org/10.1007/s11694-021-00956-0 https://doi.org/10.1007/s11694-021-01128-w https://doi.org/10.1007/s11694-021-01128-w https://doi.org/10.1016/j.bios.2021.113252 https://doi.org/10.1021/acs.est.5b05857 https://www.ijsr.net/get_abstract.php?paper_id=ART20163056 https://doi.org/10.1016/j.jelechem.2015.04.034 https://doi.org/10.1016/j.colsurfb.2010.06.020 https://doi.org/10.1016/j.chemosphere.2021.132187 https://doi.org/10.1007/s11244-021-01479-0 http://doi.org/10.1039/C0JM00549E https://doi.org/10.1039/C5CS00352K https://doi.org/10.1016/j.solmat.2015.08.036 M. Mehmandoust et al. J. Electrochem. Sci. Eng. 12(1) (2022) 91-103 http://dx.doi.org/10.5599/jese.1117 103 [43] T. Zabihpour, S.-A. Shahidi, H. Karimi-Maleh, A. Ghorbani-Hasan Saraei, Journal of Food Measurement and Characterization 14 (2020) 1039-1045. https://doi.org/10.1007/s11694- 019-00353-8 [44] M. M. Abdel-Moety, Der Pharmacia Lettre 7(9) (2015) 285-294. https://www.researchgate. net/publication/339102987_Carbon_paste_ion-selective_electrodes_for_the_determinati on_of_olopatadine_hydrochloride_in_bulk_and_pharmaceutical_dosage_forms/link/5e3d6d 88299bf1cdb9163a01/download [45] M. M. Sebaiy, M. A. Elmosallamy, M. M. Elhenawee, M. K. Alshuwaili, , Microchemical Journal 147 (2019) 170-175. https://doi.org/10.1016/j.microc.2019.03.030 [46] N. Sreedhar, A. Sreenivasulu, M. S. Kumar, M. Nagaraju, Journal of Pharmaceutical Sciences and Research 3(8) (2012) 2517-2521. http://dx.doi.org/10.13040/ IJPSR.0975-8232.3(8).2517-21 ©2021 by the authors; licensee IAPC, Zagreb, Croatia. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) http://dx.doi.org/10.5599/jese.1117 https://doi.org/10.1007/s11694-019-00353-8 https://doi.org/10.1007/s11694-019-00353-8 https://doi.org/10.1016/j.microc.2019.03.030 http://dx.doi.org/10.13040/IJPSR.0975-8232.3(8).2517-21 http://dx.doi.org/10.13040/IJPSR.0975-8232.3(8).2517-21 https://creativecommons.org/licenses/by/4.0/) https://www.researchgate. net/publication/339102987_Carbon_paste_ion-selective_electrodes_for_the_determinati on_of_olopatadine_hydrochloride_in_bulk_and_pharmaceutical_dosage_forms/link/5e3d6d 88299bf1cdb9163a01/download https://www.researchgate. net/publication/339102987_Carbon_paste_ion-selective_electrodes_for_the_determinati on_of_olopatadine_hydrochloride_in_bulk_and_pharmaceutical_dosage_forms/link/5e3d6d 88299bf1cdb9163a01/download