Standardization of stage-wise requirement of nutrients in banana cv. Grande Naine (AAA) T.N. Balamohan, J. Auxilia1 and R. Sudha2 Department of Fruit Crops Horticultural College and Research Institute Tamil Nadu Agricultural University, Coimbatore – 641 003, India E-mail: rsudhahort@yahoo.co.in ABSTRACT A field trial was conducted during 2009-2010 at College Orchard, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, to standardize stage-wise requirement of nutrients in banana cv. Grand Naine (AAA). Treatment T16 where application of 100% RDF (165:52.5:495g NPK plant -1) at 4 critical growth stages, i.e., 40:52.5:25, 30:0:35, 30:0:25 and 0:0:15% at the 3rd, 5th, 7th and 9th months after planting (MAP), respectively, recorded maximum plant height, pseudostem girth and leaf area index. Maximum bunch weight of 32.15kg was recorded in T16. Higher yield was attributed to more number of (i) hands per bunch, (ii) fingers per hand and (iii) per bunch, besides the higher average weight of the finger. Better quality fruits, with higher TSS, total sugars, low acidity and better sugar:acid blend, were obtained in T16. In treatment T16, where 100% RDF was applied, increased N, P, and K content were seen in the index leaf of the crop. Lower soil-available nutrients, viz., N, P, K, at the higher level of split-application at critical stages of the crop revealed, that, the nutrients applied were utilized efficiently. This was reflected in the better yield and quality obtained. Economics were worked out which indicated T16 as giving the highest cost:benefit ratio (1:3.97). Key words: Banana, Grande Naine, stage-wise nutrient requirement, yield, quality, cost:benefit ratio J. Hortl. Sci. Vol. 10(2):165-171, 2015 INTRODUCTION Banana, the second largest fruit crop in the world in terms of cultivation, produced in the tropical and subtropical regions, is recognized as the fourth most important food commodity in terms of gross value next only to paddy, wheat and milk products. India is the largest producer of banana in the world, accounting for about 25% of the global output. Its availability round-the-year at a reasonable cost, and its ready acceptance by all sections of the society, make it a very popular fruit. Peninsular India, especially the states of Tamil Nadu, Kerala and Karnataka, constitute an area of great diversity of both the banana and the plantain. Over five lakh small- and medium- farmers depend on banana cultivation for livelihood in our country. Though the farmers spend huge amounts on fertilizer, only 50% of the potential crop yield is realized owing to poor fertilizer use efficiency. With a growing population and enhanced awareness among public about health and nutritional aspects of banana, the country’s requirement in the next 20 years is expected to go up to 25 million tonnes. This volume of production can be achieved through increased productivity while ensuring better fertilizer use efficiency. Banana is a voracious feeder of nutrients; therefore, addition of mineral fertilizer stands to have a major effect on yield potential. Banana requires high amounts of K than N or P to ensure high yield and quality. It is estimated that a crop of 50 tonnes of banana in one hectare removes 320kg N, 32kg P2O5 and 925kg K2O every year. Hence, it is of paramount importance to maintain a high degree of soil fertility by timely and judicious application of NPK for achieving good fruit yield and quality in banana. In Tamil Nadu, nutrients are applied in three split doses during the 3rd, 5th and 7th month after planting (MAP) @ 110:35:330g/NPK/plant/year; But, under tropical conditions, soil nutrients are leached/ lost rapidly due to various factors. Therefore, it is important to apply nutrients at the critical stages of crop growth in small doses, at shorter intervals, to minimize loss of nutrients and cost of production. Though extensive information is available on nutrient requirement in banana, very little work has been done on stagewise nutrient requirement in this crop. This necessitates research on application of nutrients at various stages of crop growth to derive maximum benefit from a given quantity of 1Horticultural College and Research Institute, TNAU, Periyakulam, Tamil Nadu, India 3Central Potato Research Station, Muthorai, Ooty, The Nilgiris, Tamil Nadu, India 166 the nutrient. With this background, the present study was undertaken to standardize stagewise nutrient requirement in banana cv. Grand Naine under Coimbatore conditions to achieve improved yield and quality. MATERIAL AND METHODS Soil parameters A field experiment was conducted at the College Orchard, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, during 2009-2010. Soil type in the experimental field was clayey-loam, and its initial status revealed that it was alkaline (pH 8.41), medium in EC (0.32 dSm-1), available N (216.7kg ha-1), available K (453.60kg ha-1) and low in available P (18.32kg ha-1). The experiment was laid out using tissue-culture derived banana cv. Grand Naine (AAA). Drip irrigation was provided to the experimental plot. Experiment details The treatments consisted of three nutrient levels (L1: 60% recommended dose of fertilizer, L2: 80% recommended dose of fertilizer, and L3: 100% recommended dose of fertilizer) and four stages of nutrient application (3rd month, 5th month, 7th month and 9th month after planting). Per cent nutrients applied at each stage are given hereunder: Treatment Per cent nutrient level / stage of growth 3 MAP* 5 MAP 7 MAP 9 MAP (Vegetative (Flower-bud (Pre-flowering/ (Flowering/ stage) initiation flowering bunch stage) stage) stage) N K 2O N K 2O N K 2O N K 2O S1 10 10 40 20 30 30 20 40 S2 20 15 30 25 30 30 20 30 S3 30 20 20 30 30 30 20 20 S4 40 25 30 35 30 25 0 15 S5 50 30 20 40 20 30 10 0 S6 50 20 30 40 20 40 0 0 *MAP: Months after planting A total of 19 treatments, viz., T1 - L1S1, T2 - L1S2, T3 - L1S3, T4 - L1S4, T5 - L1S5, T6 - L1S6, T7 – L2S1, T8 - L2S2, T9 - L2S3, T10 - L2S4, T11 - L2S5, T12 - L2S6, T13 - L3S1, T14 - L3S2, T15 - L3S3, T16 - L3S4, T17 - L3S5, T18 - L3S6 and T19 – Control (Recommended dose of fertilizer @ 165: 52.5: 495g of NPK/plant/year in 4 splits at 2nd, 4th, 6th and 8th MAP) and phosphorus was applied as a single dose, i.e., at 3rd MAP. The above-stated treatments were tested in Randomized Block Design, with four replications. Each treatment was spread over a net area of 12.96m2, enclosing nine plants. All the recommended agronomic practices and plant protection measures were applied for raising the crop. Data analysis Observations on morphological characters, viz., pseudostem height and girth, leaf area index, and bunch characters, viz., bunch weight, number of hands and fingers, and, average weight of finger, were recorded as per standard procedures. Total soluble solids (TSS) were determined by using a hand refractometer. Total sugars, total acidity and Vitamin C were estimated using standard methods (AOAC, 1960). For soil nutrient analysis, soil samples were collected before planting and at harvest. Available nitrogen in the soil was estimated by the alkaline permanganate method (Subbiah and Asija, 1956), available phosphorus in soil estimated using Klett Summerson colorimeter with a red filter (Olsen et al, 1954), and, available potassium in the soil was estimated upon extraction with neutral N ammonium acetate using a flame photometer (Hanway and Heidal, 1952). For plant nutrient analysis, functional leaves were used at different stages of growth. Nitrogen content was estimated by Microkjeldahl method (Piper, 1966) and phosphorus content in the leaf was estimated using triple acid extract by vanadomolybdate phosphoric yellow colour method (Jackson, 1973). Potassium content was estimated by flame photometry (Jackson, 1973). Statistical analysis of the data was done as per Gomez and Gomez (1984). RESULTS AND DISCUSSION Morphological parameters Potential yield in banana can be achieved only by applying adequate doses of fertilizer at critical stages of plant growth. Banana crop requires a larger quantity of potassium, moderate quantity of nitrogen and a relatively lower dose of phosphorus for optimal growth and yield (Norris and Ayyar, 1942). Data on the influence of different levels of nutrients applied during various growth stages on growth characteristics in our study are presented in Table 1. Progressive increase in plant height and girth of the pseudostem from 3rd MAP up to shooting was observed in all the treatments. Among the treatments, T16 (with 100% of RDF at four different stages) registered maximum plant height and girth at 7th, 9th MAP, and at harvest. Increases in plant height and girth may be attributed to an increase in utilization of nutrients, more specifically, nitrogen. Improved nitrogen absorption ultimately led to Balamohan et al J. Hortl. Sci. Vol. 10(2):165-171, 2015 167 formation of complex nitrogenous substances like amino acids and proteins for building new tissues (Childers, 1966). Application of N at the critical growth stages, viz., early vegetative stage, flower-bud initiation and differentiation stage, and, post-shooting stage had a great influence on growth and development, plant health and yield. Increases in plant height and girth from application of nitrogen and potassium is commonly noticed in banana (Basagarahally, 1996; Shakila and Manivannan, 2001; Nalina, 2002); while, phosphorus increased the girth when applied along with K (Jagirdar and Ansari, 1966). Lowest plant height and stem girth were observed in treatment T1. This could be due to an insufficient supply of nitrogen and potassium at the initial stages of plant growth. Reddy et al (2002) reported that increases in plant height and girth may be largely due to a regular supply of higher doses of nitrogen and potassium. Though banana requires nutrient supplementation throughout its growing period, application of N and K prior to shooting, especially, during flower-bud initiation (4th-6th MAP) ensures uninhibited growth, and has a greater influence on bunch-size, number of fingers and hands per bunch, and ultimately, the yield (Simmonds, 1982). Leaf area index (LAI) is critical for maximum utilization of photosynthetically active radiation (PAR). In banana, optimum LAI should be 4 to 4.5. LAI was higher when the plants were under T17 at 3 rd MAP; T18 at 5 th MAP, and T16 at 7 th, 9th MAP and at harvest. Rapid development in leaf area was associated with higher accumulation of most of the nutrients applied. In the present study too, LAI increase was rapid between the 7th and 9th MAP as a result of 100% RDF applied at the four critical stages (T16). Baruah and Mohan (1991) showed that higher potassium application increased LAI considerably in banana cv. Jahaji (AAA group). Yield parameters Influence of various levels of nutrients applied at different growth stages on yield and quality is presented in Table 2. Among the treatments, T16 (NPK @ 165:52.5:495g per plant at four stages of growth) registered maximum bunch-weight (32.15kg), followed by T14 (27.96 kg) and T13 (27.77 kg). Increase in yield may be attributed to improved morphological traits such as plant height, girth and number of functional leaves. Better LAI, faster rate of leaf production and higher nutrient-uptake by plants were also observed in this treatment (T16). This is in conformity with Shakila (2000) and Nalina (2002). Increased yield in treatment T16 could be due to application of optimum quantity of fertilizers, well-spread at the four different critical stages of crop growth. This may lead to an increase in dry matter Table 1. Effect of various levels of fertilizer at different growth stages on growth parameters in banana cv. Grande Naine Treatment Plant height (m) Plant girth (cm) Leaf area index 3 5 7 9 A t 3 5 7 9 A t 3 5 7 9 A t MAP* M A P M A P M A P harvest M A P M A P M A P M A P harvest M A P M A P M A P M A P harvest T 1 0.76 1.64 1.78 1.86 1.87 25.21 49.25 57.188 65.42 66.72 0.46 2.43 2.56 4.39 2.75 T 2 0.79 1.74 1.81 1.92 1.95 26.42 50.61 59.683 66.63 69.21 0.48 2.48 2.69 5.04 2.82 T 3 0.78 1.72 1.79 1.92 1.96 26.19 50.37 59.467 66.63 67.63 0.48 2.48 2.60 5.00 2.81 T 4 0.77 1.71 1.82 1.89 1.92 25.98 49.98 59.271 66.50 67.25 0.48 2.45 2.59 4.76 2.77 T 5 0.82 1.75 1.83 1.94 1.97 26.77 50.94 59.717 67.25 69.63 0.49 2.53 2.71 5.13 2.84 T 6 0.83 1.78 1.87 1.95 1.98 27.06 51.45 60.292 68.21 70.21 0.49 2.55 2.73 5.17 2.88 T 7 0.83 1.83 1.88 2.04 2.04 27.35 51.91 60.717 68.96 70.92 0.49 2.70 2.82 5.41 2.94 T 8 0.84 1.84 1.95 2.03 2.05 27.67 52.34 60.758 70.58 71.46 0.50 2.71 2.82 5.43 2.94 T 9 0.83 1.82 1.89 2.01 2.04 27.19 51.83 60.642 68.46 70.58 0.49 2.61 2.75 5.40 2.93 T 1 0 0.84 1.87 1.91 2.03 2.06 27.71 52.38 61.096 70.92 71.46 0.51 2.72 2.84 5.55 2.95 T 1 1 0.85 1.92 1.99 2.06 2.08 27.90 53.19 61.396 71.50 71.83 0.51 2.79 2.95 5.71 3.14 T 1 2 0.85 1.93 1.98 2.05 2.07 28.02 55.57 61.396 71.33 71.54 0.52 2.86 2.91 5.68 3.14 T 1 3 0.85 1.95 2.03 2.06 2.10 28.15 55.98 62.588 71.83 72.25 0.52 2.95 3.12 5.92 3.15 T 1 4 0.85 1.96 2.06 2.07 2.15 28.90 56.63 62.833 72.17 72.46 0.53 2.97 3.13 5.93 3.17 T 1 5 0.85 2.01 2.02 2.06 2.10 29.40 57.31 62.202 71.63 72.17 0.54 3.00 3.05 5.80 3.15 T 1 6 0.86 2.01 2.13 2.16 2.18 29.83 57.36 64.117 74.75 75.25 0.54 3.02 3.19 6.50 3.26 T 1 7 0.92 2.02 2.05 2.07 2.09 30.27 59.90 62.846 72.88 72.68 0.58 3.05 3.14 6.05 3.22 T 1 8 0.87 2.08 2.09 2.12 2.12 30.17 62.39 63.108 73.33 73.30 0.56 3.14 3.15 6.26 3.25 T 1 9 0.85 1.91 1.97 2.05 2.06 27.77 53.10 61.292 70.96 71.46 0.51 2.73 2.86 5.57 3.02 SEd 3.74 11.48 9.61 4.91 0.10 1.38 4.31 1.50 2.54 0.04 0.05 0.27 0.24 0.58 0.24 CD 7.49 23.01 19.26 9.85 0.20 2.78 8.65 3.00 5.09 0.08 (NS) 0.54 0.49 1.17 0.47 (p=0.05) *MAP: Months After Planting Nutrient requirement in banana cv. Grand Naine J. Hortl. Sci. Vol. 10(2):165-171, 2015 168 at harvest, contributing ultimately to suoerior bunch and finger characters. Another plausible explanation is the timely availability of required amounts of nutrients for flower-bud initiation, a finding corroborated by Basagarahally (1996). In the present study, a low yield obtained at lower levels of nutrients applied (T1) is probably due to lower uptake of nutrients, consequently lower dry-matter production, As banana requires large amounts of potassium before and during the reproductive stage, application of 60% RDF is adequate for accelerating growth and development. Kholi et al (1985) reported that application of nitrogen increased biomass production in the leaves, rachis and flower buds, whereas, lack of N supply confined biomass production to the rhizome and pseudostem. In the present investigation, lower nutrient levels led to reduced leaf size, delayed flowering, reduced fruit number per bunch and fruit size. Highest yield in terms of bunch weight (32.15kg plant-1) was observed in T16. This increase was also associated with a corresponding increase in number of hands per bunch (10.18) and number of fingers per bunch (191.09). In any production system, the primary objective is to attain maximum fruit yield per unit area without affecting fruit quality. Fruit quality in banana is judged mainly by sugar content and acidity in the pulp. A marked effect on fruit quality was observed with application of adequate amount of nutrients. Higher levels of TSS (17.20%), ascorbic acid (15.07mg/100g), total sugars (20.23%), sugar/ acid ratio (183.90) and lower acidity (0.11%) were recorded in fruits under T16. Higher fruit-quality, especially sugar content, can be explained on the basis of the role of nutrients, particularly potassium, involved in carbohydrate synthesis, breakdown and translocation of starch, synthesis of protein, and, in neutralization of physiologically important organic acids (Tisdale and Nelson, 1966). Several investigations have more firmly established the involvement of K + in carbohydrate synthesis and its absolute requirement for the activity of the enzyme, starch synthetase (Greenberg and Preiss, 1965). Increased level of potassium reduced the acidity in the pulp. This may be due neutralization of organic acids from a high K+ level in the tissues (Tisdale and Nelson, 1966; Ram and Prasad, 1988). Leaf nutrient content Leaf nutrient concentration in the banana plant provides information on plant nutrient status, and reflects the optimum leaf concentration of major nutrients aiding in growth and development in the banana crop. The present study, maximum N content was registered in treatment T16 at the 7th (3.46%), T13 at the 9 th (3.49%) MAP and at harvest (Table 3). This may be due to an optimum availability of N Table 2. Effect of various levels of fertilizer at different growth stages on yield and fruit quality in banana cv. Grande Naine Treatment Bunch No. of No. of No. of Average TSS Acidity Ascorbic Total Sugar/ weight hands fingers in fingers finger (%) (%) acid sugars acid (Kg) (bunch-1) 2nd hand (bunch-1) weight (g) (mg 100 g-1) (%) ratio T 1 24.06 8.75 17.21 162.63 147.36 12.30 0.22 11.56 15.80 71.81 T 2 25.62 9.13 18.67 169.45 157.51 15.10 0.19 12.04 16.30 85.78 T 3 24.29 9.13 18.17 165.89 154.19 14.90 0.20 11.93 16.27 81.35 T 4 25.11 8.92 17.33 163.12 150.38 14.40 0.21 11.62 16.00 76.19 T 5 25.63 9.21 19.25 169.60 158.88 15.20 0.18 12.37 16.67 92.61 T 6 25.65 9.33 19.67 170.23 159.96 15.50 0.18 12.43 16.83 93.50 T 7 26.65 9.46 19.71 180.00 161.15 15.60 0.17 12.69 17.50 102.94 T 8 26.73 9.54 19.83 180.59 161.99 15.70 0.17 12.93 18.20 107.05 T 9 26.32 9.33 19.71 170.52 161.10 15.50 0.18 12.57 17.22 101.87 T 1 0 26.73 9.67 19.96 173.39 165.00 15.85 0.17 13.62 15.73 126.46 T 1 1 27.66 9.79 20.21 181.10 176.19 16.50 0.16 14.36 17.20 135.78 T 1 2 27.56 9.75 20.13 177.07 171.13 15.90 0.16 14.05 16.73 101.87 T 1 3 27.77 9.83 20.63 184.11 181.65 16.50 0.15 14.85 18.97 126.46 T 1 4 27.96 9.96 21.04 184.83 185.79 16.75 0.14 14.89 19.01 135.78 T 1 5 27.67 9.79 20.63 181.79 176.19 16.50 0.15 14.49 18.90 126.00 T 1 6 32.15 10.18 21.50 191.09 190.00 17.20 0.11 15.07 20.23 183.90 T 1 7 28.18 10.00 21.13 185.98 186.87 16.90 0.14 14.92 19.10 136.42 T 1 8 30.27 10.33 21.50 186.01 188.25 16.90 0.13 14.92 19.97 153.61 T 1 9 27.31 9.75 20.04 176.31 170.08 15.90 0.18 14.02 18.43 102.38 SEd 0.76 0.33 1.45 0.28 4.13 0.02 0.007 0.023 0.05 7.86 CD (p=0.05) 1.52 NS 2.91 0.56 8.29 0.04 0.015 0.046 0.10 16.45 Balamohan et al J. Hortl. Sci. Vol. 10(2):165-171, 2015 169 in the soil, favouring higher absorption and translocation by roots. Lower content of N in the higher dose of applied fertilizer, viz., T1 to T6, could be due to the osmotic effect, which may have hindered uptake of N and supply of nitrogen converted from the nitrate to the ammoniacal form. Also, per cent dose of fertilizer applied was lower than in T16. Phosphorus content in various plant parts at different growth stages in banana cv. Grand Naine revealed that in leaves, this nutrient increased linearly until shooting. This may be due to an increased physiological activity in the plant as development proceeded. Decrease in P content in leaves at harvest could be related to mobilization of stored assimilates to the fruit. P content was maximum in leaf lamina at the shooting stage. This indicates that P absorbed during earlier stages accumulates in the midrib and leaf lamina, thereby serving as a source during peak vegetative stages for higher photosynthetic activity. P content in the leaves decreased at harvest showing that metabolites containing P were translocated to the fruit. A higher demand for P at the early stages and at flowering (Veerannah et al, 1976) and reduction in P at harvest (Twyford and Walmsley, 1974) have been reported. In the present investigation, K content in leaves at different stages indicates that this nutrient accumulated in the vegetative parts until shooting, highlighting that K concentration increased with increased physiological activity during the developmental processes. Decrease in K content during harvest was probably due to an increased catalytic activity of K in reproductive parts for mobilizing metabolites from the vegetative part (despite substantial post-shooting uptake from the soil). Veerannah et al (1976) reported similar results in banana cv. Robusta. Among the treatments, T16 recorded highest K content at the 5th (4.10%) and 7th (4.21%) MAP. This may be due to an increase in the available form of K in the soil solution. Soil nutrient status In the present investigation, available N, P and K were estimated at planting and at harvest to study soil nutrient status under various treatments (Table 4). Initial soil N status was found to be 216.70kg/ha. Available N in the soil at harvest under different treatments was significantly variable. Treatment T1 (572.55kg/ha), followed by T4 (486.65kg/ha), recorded high amounts of available N, as, 20% was applied at the 9th MAP which was perhaps not fully utilized by the plant (since, at 9th MAP, no vegetative growth occurs, and only bunch-development and fruit-filling takes place). Lesser amount of soil N was recorded in T16 (118.15kg/ha) due to application of N upto the pre-flowering stage (7th MAP). Thus, the plant was able to utilize N optimally for production of the vegetative parts. Treatment T16 recorded higher bunch yield and had 509kg ha-1 available N (which had been applied Table 3. Effect of various levels of fertilizer at different growth stages on leaf nutrient concentration in banana cv. Grande Naine Treatment Nitrogen (%) Phosphorus (%) Potassium (%) 5 7 9 A t 5 7 9 A t 5 7 9 A t MAP* M A P M A P harvest M A P M A P M A P harvest M A P M A P M A P harvest T 1 2.46 2.53 2.97 2.97 0.172 0.497 0.635 0.137 3.41 3.67 4.42 3.75 T 2 2.62 2.78 2.91 2.82 0.184 0.509 0.649 0.141 3.54 3.74 4.39 3.73 T 3 2.12 2.83 2.89 2.81 0.181 0.504 0.642 0.140 3.42 3.69 4.20 3.69 T 4 2.71 2.91 2.76 2.59 0.179 0.498 0.639 0.138 3.55 3.69 4.34 3.71 T 5 2.85 2.83 2.87 2.76 0.189 0.509 0.651 0.145 3.55 3.81 4.24 3.67 T 6 2.86 2.96 2.62 2.73 0.192 0.511 0.653 0.147 3.42 3.81 4.18 3.72 T 7 2.97 3.05 3.25 3.14 0.196 0.514 0.657 0.149 3.60 3.87 4.53 3.83 T 8 2.99 3.06 3.21 3.11 0.197 0.514 0.657 0.151 3.61 3.92 4.56 3.84 T 9 2.89 3.06 3.19 3.09 0.194 0.514 0.654 0.149 3.56 3.82 4.51 3.79 T 1 0 3.01 3.15 3.11 3.03 0.198 0.516 0.657 0.151 3.72 3.96 4.47 3.78 T 1 1 3.04 3.12 3.18 3.06 0.211 0.517 0.663 0.155 3.81 4.03 4.43 3.78 T 1 2 3.07 3.16 3.09 3.05 0.213 0.517 0.662 0.153 3.67 4.01 4.46 3.79 T 1 3 3.09 3.22 3.49 3.37 0.213 0.519 0.671 0.156 3.84 4.11 4.52 3.81 T 1 4 3.12 3.23 3.42 3.29 0.213 0.520 0.674 0.156 3.85 4.11 4.63 4.04 T 1 5 3.14 3.25 3.39 3.23 0.214 0.518 0.664 0.156 4.02 4.09 4.59 3.91 T 1 6 3.18 3.46 3.29 3.15 0.215 0.524 0.698 0.164 4.10 4.21 4.58 4.10 T 1 7 3.21 3.32 3.39 3.22 0.215 0.521 0.678 0.158 3.68 4.17 4.69 3.89 T 1 8 3.24 3.39 3.29 3.17 0.217 0.521 0.684 0.159 4.09 3.97 4.58 3.84 T 1 9 3.16 3.39 3.26 2.73 0.204 0.516 0.659 0.151 4.10 4.17 4.58 3.87 SEd 0.005 0.004 0.004 0.005 0.002 0.0009 0.002 0.002 0.005 0.004 0.004 0.003 CD (p=0.05) 0.012 0.009 0.008 0.009 (NS) (NS) (NS) (NS) 0.009 0.008 0.008 0.006 *MAP: Months After Planting Nutrient requirement in banana cv. Grand Naine J. Hortl. Sci. Vol. 10(2):165-171, 2015 170 in three splits). This may be due to utilization of N, by the plant during its critical stages of growth, in three split applications. This may have facilitated optimum N concentration in the soil for the entire cropping period. Therefore, residual nutrients in the soil may be subjected to lower loss. Phosphorus estimated at the initial stages and at harvest revealed that available P in the soil showed a decreasing trend, which may be due to a continuous uptake of this nutrient by the plant. However, differences among treatments were not significant. Uptake of P was also influenced substantially by potassium application. Application of fertilizers also altered available K in the soil. Available K in the soil at harvest showed a decreasing trend with time, and the quantity applied. Among the various treatments, available K in soil was lower in T5 (475.6kg/ha) and T6 (457.89kg/ha) at 60% RDF; in T11 (385.78kg/ha) and T12 (385.85kg/ha) at 80% RDF, and, in T18 (295.78kg/ha) at 100% RDF at harvest. K content in soil in T16 treatment was lower than in treatment T19 (in which the same dose of fertilizer was applied in four equal splits). Higher application of potassium in the early stages improved growth, yield and quality attributes. As, the time of application and stage of plant growth determines uptake and translocation of K, application of this nutrient at four critical stages may have provided optimal availability of K in the soil, throughout the growth period, and facilitated better growth. Similarly, higher K uptake for bunch-development and finger-filling also resulted in lower levels of K in soil at harvest. Application of 100% RDF in T16 (165:52.5:495 g NPK plant-1 at 4 critical growth stages, i.e., 40:100:25, 30:0:35, 30:0:25, 0:0:15% at 3rd, 5th, 7th and 9th MAP, respectively) recorded highest cost:benefit ratio (1:3.97). The highest gross and net returns realized were because of highest yield. As, the cost of cultivation was also equal to that in the Control, application of fertilizer this way was found to be economically more viable. REFERENCES A.O.A. C. 1960. Official Methods of Analysis. A.O.A.C., Washington D.C., USA Basagarahally, R. 1996. Micropropagation and nutritional studies of tissue cultured banana cv. Grand Naine. Ph.D. Thesis, University of Agricultural Science, Bangalore, Karnataka, India Baruah, P.J. and Mohan, N.K. 1991. Effect of potassium on leaf area index, phyllochron and number of leaves of banana cv. Jahaji. Banana Newslett., 14:21-22 Childers, N. F. 1966. Fruit Nutrition. Horticulture Publication, New Brunswick, New Jersey, USA Gomez, K.A. and Gomez, A.A. 1984. 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Standardization of fertilizer requirement Table 4. Effect of various levels of fertilizer on soil NPK (kg/ha) during harvest in banana cv. Grande Naine Treatment Nitrogen Phosphorus Potassium (kg/ha) (kg/ha) (kg/ha) T1 572.55 121.67 626.64 T2 453.65 112.57 497.46 T3 460.25 114.71 532.75 T4 486.65 118.63 573.70 T5 381.76 108.36 475.6 T6 375.48 105.45 457.89 T7 286.38 98.62 430.23 T8 276.89 97.83 429.48 T9 345.89 104.23 453.76 T10 265.70 97.20 420.76 T11 221.65 91.67 385.78 T12 228.45 93.30 385.85 T13 183.45 87.57 372.83 T14 176.65 83.27 358.70 T15 198.32 89.61 372.94 T16 118.15 76.25 270.6 T17 169.97 82.56 343.67 T18 168.35 78.56 295.78 T19 249.75 96.81 419.53 SEd 2.34 0.24 1.63 CD (p=0.05) 4.66 N 3.27 Balamohan et al J. Hortl. Sci. Vol. 10(2):165-171, 2015 171 (MS Received 09 June 2014, Revised 08 June 2015, Accepted 20 June 2015) for tissue cultured banana cv. Robusta (AAA). Ph.D. Thesis, Tamil Nadu Agricultural University, Coimbatore, India Norris, R.V. and Ayyar, C.V.R. 1942. 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