Final SPH -JHS Coverpage 17-1 Jan 2022 single J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 This is an open access article d istributed under the terms of Creative Commons Attribution-NonCommer cial-ShareAl ike 4.0 International License, which permits unrestricted non-commercial use, d istribution, and reproduction in any med ium, provide d the original author and source are credited. Original Research Paper INTRODUCTION Jasmine (Jasminum spp.) is one of the remuneratively prized and significant traditional flower crops of India. It belongs to the family Oleaceae and is one of the aromatic flowers cultivated since times immemorial and is considered as the most revered flower in our country for its attractive and fragrant flowers. Jasmine flowers are popularly used in preparation of garlands, hair adornments for women, used in religious and ceremonial occasion and also for extracting perfumery oil (Sanchita et al., 2018) which is used in the cosmetic and perfumery industries. India is the largest exporter of jasmine oil in the world accounting for over 40 per cent of total world export. It has extensive application in aromatherapy as jasmine fragrance is effective in treating depression, nervous exhaustion and stress. It is also widely used in the medicinal and pharmaceutical industries (Green and Miller, 2009). Exceptional increase in the consumption of jasmine flowers by the Indian population settled in Middle East countries and the United States of America has led to the augmentative export demand for flower strings of J. sambac (Jawaharlal et al., 2012). The genus Jasminum is reported to comprise of around 200 species (Bailey 1958). The commercially cultivated jasmine species in Tamil Nadu, Karnataka, Andhra Pradesh, Uttar Pradesh and some parts of Bihar and West Bengal are J. sambac, J. grandiflorum, J. auriculatum and J. multiflorum. Exclusive of these commercially important species, lesser-known species namely, J. nitidum, J. calophyllum and J. flexile also acquire economic importance as they produce flowers which are suitable for use as loose flower, besides being ideal garden plants (Raman, 1969; Ganga et al., 2015). Hybridization leads to the development of new adaptive traits allowing the expansion of new habitats (Johnston et al., 2004), fitness enhancement (Burke et al., 1998), or the origin of new hybrid lineages Impact of pollination strategies on fruit set and fruit growth attributes in jasmine Usha S.1*, Ganga M.1, Rajamani K.2, Manonmani S.2 and Gnanam R.3 1 Department of Floriculture and Landscape Architecture, 2 Centre for Plant Breeding and Genetics, 3 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India Corresponding author E-Mail: usha.annapoorna@gmail.com ABSTRACT Jasmine occupies predominant position among the flower crops in India in terms of area, production and productivity. The demand for jasmine flowers is growing day by day owing to its wide range of uses and there is a pressing need for improving the crop by exploring strategies to evolve diverse genotypes. The present study focuses on the hybridization of Jasminum spp with the objective of introgression of desirable traits that would aid in creation of wider genetic variability. Pollination is the basis in any hybridization programme. The main aim of this research study was to determine the suitable pollination methods among self, open and cross pollination and to assess the effect of the pollination methods on the fruit set and fruit characteristics. The results of the study revealed that the overall response of J. auriculatum was found effective with maximum fruit set percentage. J. auriculatum cv Parimullai yielded the highest fruit set of 76.43% under open pollination and the least fruit set rate of 2.14% under self-pollination. Among the possible cross combination involving J. auriculatum and J. grandiflorum cultivars as seed parents with various pollen parents, J. flexile showed considerable results. Cross combination of J. auriculatum x J. flexile recorded maximum fruit set revealing best cross compatibility while crosses involving J. sambac resulted in no fruit set indicating the prevalence of fertilization barriers that hinder hybridization. Keywords: Fruit set, fruit growth, jasmine and pollination 74 J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 (Grant, 1981; Arnold, 1997). Hybridization can also reinforce reproductive bar riers through natural selection for conspecific gene flow (Arnold, 1992), the creation of stable hybrid zones (Barton and Hewitt, 1985), or the for mation of introgressive ra ces. Hybridization is not the general outcome whenever congeneric species come into contact because there often are pre- and post-mating barriers that prevent hybridization. Pollination is the result of pollen being transferred from the anther to the stigma of another flower. Landing of pollen on stigma is no guarantee for seed- set. Failure of fertilization after self-pollination in self- sterile or self-incompatible plants may also be due to the inability of the pollen to germinate on its own stigma. Pollination in many crops has manifested a major influence on the number of fruit set, fruit length, fruit girth and fruit shape (Nirmalaruban et al., 2020). Jasmine varieties released till date are only clonal selections and mutants. Hence there is a dire necessity to evolve hybrids in jasmine using commercial and under-utilized species. Prior attempts at hybridization ha ve fa iled beca use of the compa tibility a nd fertilization barriers present among the species. Considering the above, the present study focuses on the method of pollination and its potency on fruit set in jasmine. MATERIALS AND METHODS The study was carried out at the Department of Floriculture and Landscape Architecture, Horticultural College a nd Resea r ch Institute, Ta mil Na du Agricultural University, Coimbatore during 2019- 2021. Ten-year-old plants of J. auriculatum cultivars CO. 1 Mullai, CO.2 Mulla i, Pa rimullai a nd J. grandiflorum cultivars CO.1 Pitchi and CO.2 Pitchi wer e selected as female pa r ent a nd Ja sminum genotypes like J. auriculatum cv CO.1 Mullai, J. grandiflorum cv CO. 1 Pitchi, J. sambac, J. multiflorum, J. nitidum, J. calophyllum and J. flexile were used as the pollen source. The experimental layout was a complete randomized design with three replications of each crossing combination. The pollen from the previously bagged flowers was collected from the male parents during 6:00 to 8:00 am in the morning of the succeeding day. Similarly in the female parent, fully opened flowers and mature buds at pre anthesis stage were emasculated between 7.00 to 10.00 am. The pollen collected from the pollen source was dusted on the stigmatic surface of the respective emasculated female parent and the flowers were bagged with butter paper cover and then labelled. For the self-pollination treatment hundred flowering shoots were randomly selected on the plants and one third of the flowering bunches were bagged without any emasculation. The remaining flowering shoots (five per plant) were tagged and the flower bunches on each shoot were thinned to five buds (approx. 24 h before anthesis) for open pollination the flowers were left untreated without any bagging. The observations on days to fruit initiation, fruit set percentage, duration of fruit retention, shape of the fruit, colour of the fruit, fruit intensity and season of fruit set were recorded. Colour was assessed for each fruit using RHS colour chart. For the analysis of embryo viability, the longitudinally dissected fruits were treated with 2, 3, 5 triphenyl tetrazolium chloride and after 3 hours of incubation the stained embryos were examined for viability. Fruit set and fruit quality characters were evaluated by variance analysis using SPSS 28.0 software. RESULTS AND DISCUSSION Evaluation of the best possible cross combination with the varied method of pollination is the base factor that decides the success of a hybridization programme. The method of pollination plays a significant role in the fruit set of the plant and is influenced by various factors such as morphology of the flower, pollen-pistil interaction, nutrients and environmental parameters. As observed from the data (Table 1), among the possible cross combinations of pollen parents with completely opened flowers of J. auriculatum cultivars, the cultivar CO.1 Mullai as female parent recorded good response with male parent J. Flexile by recording the earliest fruit set (38 DAP), highest number of fruits set at 60 DAP (52), fruit set at maturity (31), highest fruit set (38.75%) and maximum fruit retention in the plant (30 days). Similarly, CO.2 Mullai produced best results as female parent when crossed with J. flexile with maximum fruit set (46.25%) but recorded delayed fruit set (47 days). The cultivar Parimullai as female parent also responded with J. flexile as pollen parent showing maximum fruit set of 48.75% and the duration of fruit retention clocked up to 33 days. The results furnished (Table 1) signify the best cross combination for the bud pollination of J. auriculatum cultivars with various pollen parents. CO.1 and CO.2 Mullai as female parents exhibited best results with Usha et al 75 H an d po lli na ti on o f co m pl et el y op en f lo w er s B ud p ol lin at io n C O .1 M ul la i M al e pa re nt N o. o f In it ia ti on N o. o f N o. o f F ru it D ur at io n N o. o f In it ia ti on N o. o f N o. o f F ru it D ur at io n fl ow er s of f ru it fr ui ts fr ui ts se t of f ru it bu ds of f ru it fr ui ts s et fr ui ts se t of f ru it po lli na te d se t (D A P ) se t at at (% ) re te nt io n P ol lin at ed se t (D A P ) at 6 0 at (% ) re te nt io n 60 m at ur it y (d ay s) D A P m at ur it y (d ay s) D A P J. g ra nd ifl or um 11 5 45 93 28 24 .3 4 24 12 5 36 11 4 62 49 .6 0 32 cv . C O .1 Pi tc hi J. s am ba c 60 - - - - - 10 0 - - - - - J. m ul tif lo ru m 10 0 52 79 34 34 .1 0 30 15 0 32 12 3 52 34 .6 7 30 J. n iti du m 15 0 38 83 38 25 .3 4 24 15 0 36 11 9 58 38 .6 7 35 J. c al op hy llu m 10 0 46 64 27 27 .1 0 28 80 30 68 33 41 .2 5 30 J. f le xi le 80 38 52 31 38 .7 5 30 80 37 72 42 52 .5 0 32 C O .2 M ul la i J. g ra nd ifl or um 11 5 41 96 25 21 .7 3 27 12 5 38 11 8 67 53 .6 1 33 cv .C O .1 Pi tc hi J. s am ba c 60 - - - - - 10 0 - - - - - J. m ul tif lo ru m 10 0 55 82 36 36 .1 0 28 15 0 30 13 4 56 37 .3 4 32 J. n iti du m 15 0 49 86 40 26 .6 7 24 15 0 38 12 9 63 42 .1 3 35 J. c al op hy llu m 10 0 41 66 28 28 .1 0 30 80 32 74 38 47 .5 0 30 J. f le xi le 80 47 54 37 46 .2 5 30 80 39 78 45 56 .2 5 33 P ar im ul la i J. g ra nd ifl or um 11 5 48 10 2 27 23 .4 7 29 12 5 34 11 6 73 58 .4 0 35 cv . C O .1 Pi tc hi J. s am ba c 60 - - - - - 10 0 - - - - - J. m ul tif lo ru m 10 0 55 87 38 38 .1 0 30 15 0 30 12 7 61 40 .6 7 34 J. n iti du m 15 0 40 92 43 28 .6 7 25 15 0 34 12 2 68 45 .3 4 38 J. c al op hy llu m 10 0 49 70 30 30 .1 0 32 80 29 74 31 38 .7 5 32 J. f le xi le 80 41 57 39 48 .7 5 33 80 35 70 42 52 .5 0 35 Ta bl e 1. I nt er s pe ci fic o f J. a ur ic ul at um c ul tiv ar s w ith v ar io us p ol le n pa re nt s J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 Impact of pollination strategies on fruit set and fruit growth attributes in Jasmine 76 H an d po lli na ti on o f co m pl et el y op en f lo w er s B ud p ol lin at io n C O .1 P it ch i M al e pa re nt N o. o f In it ia ti on N o. o f N o. o f F ru it D ur at io n N o. o f In it ia ti on N o. o f N o. o f F ru it D ur at io n fl ow er s of f ru it fr ui ts fr ui ts ` = se t of f ru it bu ds of f ru it fr ui ts s et fr ui ts se t of f ru it po lli na te d se t (D A P ) se t at at (% ) re te nt io n P ol lin at ed se t (D A P ) at 6 0 at (% ) re te nt io n 60 m at ur it y (d ay s) D A P m at ur it y (d ay s) D A P (m al fo rm ed ) J. au ri cu la tu m cv . 13 5 22 10 6 98 72 .5 9 48 15 0 25 13 8 12 4 82 .6 7 51 C O .1 M ul la i J. s am ba c 10 0 - - - - - 10 0 - - - - - J. m ul tif lo ru m 15 0 34 13 7 11 8 57 .3 4 52 15 0 32 13 3 12 5 83 .3 4 57 J. n iti du m 12 0 28 93 86 76 .6 7 39 15 0 24 12 7 11 4 76 .1 2 45 J. c al op hy llu m 80 25 62 43 53 .7 5 41 80 24 67 57 71 .2 5 47 J. f le xi le 80 22 71 64 80 .1 0 57 80 20 74 68 85 .2 1 62 C O .2 P it ch i J. au ri cu la tu m cv . 13 5 28 12 6 10 9 80 .7 4 45 15 0 20 12 6 11 9 79 .3 4 50 C O .1 M ul la i J. s am ba c 10 0 - - - - - 10 0 - - - - - J. m ul tif lo ru m 15 0 30 12 9 11 4 76 .1 6 58 15 0 29 13 7 13 2 88 .1 4 54 J. n iti du m 12 0 30 11 6 93 77 .5 0 45 15 0 22 13 1 11 8 78 .6 7 49 J. c al op hy llu m 80 24 66 45 56 .2 5 46 80 24 72 54 67 .5 3 53 J. f le xi le 80 22 75 67 83 .7 5 45 80 21 75 66 82 .5 4 60 D A P – D ay s af te r po lli na tio nTa bl e 2. I nt er s pe ci fic h yb ri di za tio n of J . g ra nd if lo ru m c ul tiv ar s w ith v ar io us p ol le n pa re nt s J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 Usha et al 77 the combination of J. flexile as pollen parent while Parimullai responded well with the pollen parent J. grandiflorum CO.1 Pitchi. The results emphasize the fact that the pollen source and quantity influence the fruit set. Aggregated results from the Table 1 indicate that there is significant amount of fruit set failure from the fruit set at 60 DAP till maturity. The failure in fruit development or the malformation of fruits accounts to the low fertilization rate (Koubouris et al., 2010) or loss of pollen viability (Deng et al., 2017) or inadequate nutrient availability (Nyomora et al., 1999). Competition between fruits for assimilates and growth regulators are the factors that are responsible for different fruiting behaviour of the assessed cultivars. In crosses involving J. grandiflorum cultivars as female parents, CO.1 Pitchi and CO.2 Pitchi evinced best results with J. flexile as the pollen donor. Maximum fruit set (80.10 and 83.75% respectively in CO.1 Pitchi and CO.2 Pitchi) with the earliest fruit set initiation of 22 days were recorded for the crosses effected with hand pollination of open flowers. For the bud pollination, CO.1 Pitchi had best compatibility with J. flexile while CO.2 Pitchi proved the best results with the combination that entailed J. multiflorum as the pollen parent (Table 2). The major drawback in the crosses involving J. grandiflorum as seed setting parent is the abnormal fruit set. The initiation of the fruit set is expressed by the bulging of the ovary proving the development of the fruit but as time progresses the ovary fails to develop completely causing misshapen fruits further arresting the growth of the embryo resulting in the loss of fruit set. Existence of pre-fertilization barriers like low pollen viability, early senescence of pistil cells and low pistil receptivity are the possible barriers in hybrid set (Deng et al., 2016). Early and rapid senescence of pistils is harmful for pollen adhesion and germination resulting in the arrest of pollen tube growth after it enters the stigma. Hybrid sterility can also be accounted due to the structural changes in the chromosomes (Sharma and Sharma, 1958). None of the crosses involving J. sambac as male parent resulted in fruit set both in hand pollination and bud pollination implying that prevalence of pre- fertilization barriers hinders the fruit set. Low pollen fer tility, pistil r eceptivity a nd pollen-stigma compatibility, ovule sterility (Deng et al., 2010; Sua ŕez et al., 2012) have been enumerated as major reasons responsible for the hampered hybrid set. With respect to open pollination J. auriculatum cv Parimullai recorded maximum fruit set of 76.43% with the earliest fruit initiation of 32 days and retained the fruits up to 28 days (Table 3.) while J. grandiflorum cv CO.2 Pitchi proved best with the highest fruit set (83.40%), earliest initiation of fruit set (38 days) and longest duration of fruit retention(55 days) although malformation of the fruits occurred during their growth stage. The favourable fruit set in J. auriculatum may be a ttr ibuted with a s the a bsence of embr yo antagonism (Veluswamy et al., 1981) and better source-sink relationship supporting the nutrient availability (Keshavarz et al., 2011). Failure in the fruit development and maturity can also be caused due to the abnormalities in the endosperm. Irregularities in the endosperm result in embryo starvation leading to distorted embryo sac (Veluswamy et al., 1981). Along with pre-fertilization barriers, obstructions post fertilization also poses a threat in hybridization. Data in Table 3 are pertinent to self-pollination in J. auriculatum and J. grandiflorum. The cultivars CO.2 Mullai, CO.1 Mullai and Parimullai of J. auriculatum recorded fruit set rates of 20.86 %, 8.16 % and 2.14 % respectively. Thus, the results revealed that J. grandiflorum exhibited better self-pollina tion efficiency in comparison with J. auriculatum but the fruit malformation in J. grandiflorum stands as a stumbling block the hybridization attempts involving this species. Data furnished in Table 4 demonstrated that fruits evolved from crosses involving J. multiflorum and J. nitidum exhibited oblate shape while those from the crosses involving J. grandiflorum cv CO.1 Pitchi, J. calophyllum and J. flexile expressed spherical shape. Fruit intensity was profuse for most of the cross combinations in bud pollination when compared to hand pollination of the open flowers. Peak season of fruit set concurred with June to November under both the pollination methods. J. flexile and J. multiflorum as pollen parents responded well with Parimullai as female parent in terms of fruit growth (Table 5). Colour of the fruit varied from light green to yellow green and medium green and turns black on maturity. Fruits of J. auriculatum yielded from open pollination performed better in terms of growth as well as the intensity of the fruit set while self-pollinated fruits J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 Impact of pollination strategies on fruit set and fruit growth attributes in Jasmine 78 Table 3: Open and self-pollination of J. auriculatum and J. grandiflorum cultivars Cultivars No. of Initiation No. of No. of Fruit Duration flowers of fruit fruits fruits at set of fruit pollinated set set at maturity (%) retention (DAP) 60 (days) DAP Open pollination J. auriculatum CO.1 Mullai 250 46 164 138 55.20 28 J. auriculatum CO.2 Mullai 235 41 183 169 71.91 24 J. auriculatum Parimullai 250 32 217 191 76.43 28 J. grandiflorum CO.1 Pitchi 235 45 204 189 80.42 52 J. grandiflorum CO.2 Pitchi 235 38 228 196 83.40 55 Self-pollination J. auriculatum CO.1 Mullai 150 43 38 12 8.16 26 J. auriculatum CO.2 Mullai 115 40 76 24 20.86 24 J. auriculatum Parimullai 150 42 91 30 2.14 25 J. grandiflorum CO.1 Pitchi 115 42 97 74 64.34 57 J. grandiflorum CO.2 Pitchi 115 40 103 81 70.43 58 DAP- Days after pollination proved better in terms of fruit growth with bolder fruits though the fruit set was poor. The efficiency of the fruit set depends upon the flowers that have pollen- laden anthers that appear to set fruit far better when cross- pollinated than when fertilized with their own pollen (Ortega et al., 2006). Despite the lack of complete fruit development in J. grandiflorum, peak season of the fruit set was observed during February to April. The fruits were conical in shape and yellow- green in colour (Table 6). In terms of method of pollination, open pollination contributed the most for the successful fruit set followed by bud pollination (Fig 1.). Results pertaining to fruit growth and quality parameters (Fig 2.) revealed that bud pollination followed by ha nd pollina tion of open flower s ensured significantly superior fruit set. Fig. 1. Effect of various pollination methods on fruit set in Jasminum spp Fig. 2. Effect of various pollination methods on fruit intensity in Jasminum spp Among all the possible cross combinations J. flexile cor r esponded well with all the cultiva rs of J. auriculatum and can be considered as the best pollen donor parent for the successful hybridization of the crop. J. auriculatum cv. Parimullai provided best results among all the cultivars in terms of fruit set and intensity , thus proving to be an elite female parent amongst the cross combinations. This study J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 Usha et al 79 H an d po lli na ti on o f co m pl et el y op en f lo w er s B ud p ol lin at io n C O .1 M ul la i M al e pa re nt F ru it Se as on Sh ap e C ol ou r F ru it F ru it F ri t Se as on Sh ap e C ol ou r F ru it F ru it In te ns it y of f ru it of t he of t he le ng th gi rt h in te ns it y of f ru it of t he of t he le ng th gi rt h se t fr ui t fr ui t (c m ) (c m ) se t fr ui t fr ui t (c m ) (c m ) J. g ra nd ifl or um V er y sp ar se Ju n- N ov Sp he ri ca l M ed iu m 1. 06 1. 45 M od er at e Ju n- N ov Sp he ri ca l M ed iu m 1. 16 1. 35 cv .C O .1 Pi tc hi gr ee n gr ee n J. m ul tif lo ru m M od er at e Ju n- O ct O bl at e L ig ht 1. 12 1. 28 M od er at e Ju n- O ct O bl at e L ig ht 1. 21 1. 38 gr ee n gr ee n J. n iti du m Sp ar se Ju n- O ct O bl at e L ig ht 1. 09 1. 31 M od er at e Ju n- O ct O bl at e L ig ht 1. 11 1. 31 gr ee n gr ee n J. c al op hy llu m Sl ig ht ly Ju n- N ov Sp he ri ca l Y el lo w 1. 04 1. 28 M od er at e Ju n- N ov Sp he ri ca l Y el lo w 1. 06 1. 28 sp ar se gr ee n gr ee n J. f le xi le M od er at e Ju n- N ov Sp he ri ca l Y el lo w 1. 17 1. 30 Pr of us e Ju n- N ov Sp he ri ca l Y el lo w 1. 15 1. 32 gr ee n gr ee n C O .2 M ul la i J. g ra nd ifl or um V er y sp ar se Ju n- N ov Sp he ri ca l M ed iu m 1. 03 1. 24 Pr of us e Ju n- N ov Sp he ri ca l M ed iu m 1. 16 1. 35 cv . C O .1 Pi tc hi gr ee n gr ee n J. m ul tif lo ru m M od er at e Ju n- O ct O bl at e L ig ht 1. 16 1. 28 M od er at e Ju n- O ct O bl at e L ig ht 1. 21 1. 38 gr ee n gr ee n J. n iti du m Sp ar se Ju n- O ct O bl at e L ig ht 1. 08 1. 25 Sl ig ht ly p ro fu se Ju n- O ct O bl at e L ig ht 1. 11 1. 31 gr ee n gr ee n J. c al op hy llu m Sp ar se Ju n- N ov Sp he ri ca l Y el lo w 1. 12 1. 28 Sl ig ht ly p ro fu se Ju n- N ov Sp he ri ca l Y el lo w 1. 06 1. 28 gr ee n gr ee n J. f le xi le M od er at e Ju n- N ov Sp he ri ca l Y el lo w 1. 21 1. 42 Pr of us e Ju n- N ov Sp he ri ca l Y el lo w 1. 15 1. 32 gr ee n gr ee n Ta bl e 4. A na ly si s of fr ui t ch ar ac te ri st ic s fo r cr os s co m bi na tio n of J . a ur ic ul at um C O .1 a nd C O .2 M ul la i c ul tiv ar s w ith v ar io us p ol le n pa re nt s J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 Impact of pollination strategies on fruit set and fruit growth attributes in Jasmine 80 H an d po lli na ti on o f co m pl et el y op en f lo w er s B ud p ol lin at io n P ar im ul la i M al e pa re nt F ru it Se as on Sh ap e C ol ou r F ru it F ru it F ri t Se as on Sh ap e C ol ou r F ru it F ru it In te ns it y of f ru it of t he of t he le ng th gi rt h in te ns it y of f ru it of t he of t he le ng th gi rt h se t fr ui t fr ui t (c m ) (c m ) se t fr ui t fr ui t (c m ) (c m ) J. g ra nd ifl or um Sl ig ht ly Ju n- N ov Sp he ri ca l M ed iu m 1. 09 1. 37 Pr of us e Ju n- N ov Sp he ri ca l M ed iu m 1. 16 1. 35 cv . C O 1 P itc hi sp ar se gr ee n gr ee n J. s am ba c N il - - - - - N il - - - - - J. m ul tif lo ru m M od er at e Ju n- O ct O bl at e L ig ht 1. 15 1. 47 M od er at e Ju n- O ct O bl at e L ig ht 1. 21 1. 38 gr ee n gr ee n J. n iti du m Sp ar se Ju n- O ct O bl at e L ig ht 1. 06 1. 42 Sl ig ht ly Ju n- O ct O bl at e L ig ht 1. 11 1. 31 gr ee n pr of us e gr ee n J. c al op hy llu m Sp ar se Ju n- N ov Sp he ri ca l Y el lo w 1. 12 1. 28 Sl ig ht ly Ju n- N ov Sp he ri ca l Y el lo w 1. 06 1. 28 gr ee n pr of us e gr ee n J. f le xi le M od er at e Ju n- N ov Sp he ri ca l Y el lo w 1. 21 1. 42 Pr of us e Ju n- N ov Sp he ri ca l Y el lo w 1. 15 1. 32 gr ee n gr ee n Ta bl e 5. A na ly si s of f ru it ch ar ac te ri st ic s fo r cr os s co m bi na tio n of J . a ur ic ul at um c v. P ar im ul la i a s fe m al e pa re nt w ith v ar io us p ol le n pa re nt s J. Hortl. Sci. Vol. 17(1) : 73-82, 2022 Usha et al 81 Table 6: Analysis of fruit characteristics for open pollinated and self-pollinated J. auriculatum and J. grandiflorum cultivars Cultivars Fruit Season Shape Colour Fruit Fruit Intensity of fruit of the of the length girth set fruit fruit (cm) (cm) Open Pollination J. auriculatum CO.1 Mullai Profuse Jun-Nov Spherical Medium green 1.09 1.31 J. auriculatum CO.2 Mullai Profuse Jun-Nov Spherical Light green 1.12 1.35 J. auriculatum Parimullai Profuse Jun-Nov Spherical Medium green 1.18 1.46 J. grandiflorum CO.1 Pitchi Highly Feb-Apr Conical Yellow green 0.53 0.31 profuse J. grandiflorum CO.2 Pitchi Highly Feb-Apr Conical Yellow green 0.51 0.46 profuse Self-Pollination J. auriculatum CO.1 Mullai Moderate Jun-Oct Oblate Light green 1.16 1.28 J. auriculatum CO.2 Mullai Sparse Jun-Oct Oblate Light green 1.08 1.25 J. auriculatum Parimullai Sparse Jun-Nov Spherical Yellow green 1.12 1.28 J. grandiflorum CO.1 Pitchi Moderate Jun-Nov Spherical Yellow green 1.21 1.42 J. grandiflorum CO.2 Pitchi Moderate Jun-Oct Oblate Light green 1.16 1.28 indicates the failure of fruit set and fertilization barrier prevailing in jasmine upon hybridization. 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Morphological, histological and ultrastructural changes in the olive pistil during flower ing. Sex Plant Reprod 25: 133–146. (Received: 09.03.2022 ; Revised:11.07.2022; Accepted:09.08.2022 ) 00 A Final SPH -JHS Coverpage First 2 pages.pdf 00 Content and in this issue.pdf 01 Mohan Kumar G N.pdf 02 Meera Pandey.pdf 03 Biradar C.pdf 04 Varalakshmi B.pdf 05 Vijayakumari N.pdf 06 Barik S.pdf 07 Sajid M B.pdf 08 Ranga D.pdf 09 Usha S.pdf 10 Manisha.pdf 11 Amulya R N.pdf 12 Akshatha H J.pdf 13 Adak T.pdf 14 Sujatha S.pdf 15 Gowda P P.pdf 16 Subba S.pdf 17 Dhayalan V.pdf 19 Ahmed S.pdf 20 Vishwakarma P K.pdf 21 Deep Lata.pdf 22 Udaykumar K P.pdf 23 Nayaka V S K.pdf 24 Sahel N A.pdf 25 Bayogan E R V.pdf 26 Rathinakumari A C.pdf 27 Yella Swami C.pdf 28 Saidulu Y.pdf 29 Sindhu S.pdf 30 Neeraj.pdf 31 Sivaranjani R.pdf 32 Rashied Tetteh.pdf 34 Sangeetha G.pdf 35 Shareefa M.pdf 36 Last Pages.pdf