45 J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 Original Research Paper Variability and Genetic Divergence in Vegetable Cowpea Germplasm of Goa Thangam M.1, Ramachandrudu K.2, Ashok Kumar J.3, Safeena S.A.4 and Priya Devi S.1 1ICAR –Central Coastal Agricultural Research Institute, Ela, Old Goa - 403 402, Goa, India 2 ICAR Directorate of Oil Palm Research, Pedavegi - 534 450, West Godavari, Andhra Pradesh 3 ICAR Central Institute of Brackishwater Aquaculture, Chennai - 600 028, Tamil Nadu 4ICAR- Directorate of Floricultural Research, Shivajinagar, Pune, Maharashtra, India Email : thangamgoa@gmail.com, thangam.m@icar.gov.in ABSTRACT Vegetable cowpea or Yard long bean [Vigna unguiculata var. sesquipedalis L. (Walp)] is a warm season leguminous crops grown especially for vegetable purpose along the west coast of India. In Goa, pole type varieties are preferred over bushy types as they offer multiple harvests with comparatively longer pods. There is wide variability found for different morphological and other traits in the local types cultivated in the state of Goa. Exploration of genetic variability in the available germplasm is a prerequisite for initiation of any successful breeding programme. Twenty nine genotypes of vegetable cowpea including three improved varieties collected from different parts of Goa state were evaluated for twelve quantitative characters including yield. High variability was observed for pod yield/plant, number of pods/plant and pod length. The high variability for pod yield per plant is apparent as the pod yield ranged from 315.25 to 2070.45 g/plant with an average of 827.48 g per plant. Pod yield depends on number of pods per plant, pod length and pod weight. Number of pods per plant ranged from 36.65 to 147.80. Pod weight depends on pod length, number of seeds per pod and hundred seeds weight. Wide variation was observed for all these characters in the present study. The GCV value was maximum for pod yield per plant (g) followed by pod weight (g) and number of pods per plant. Low values of GCV were observed for days to first flowering, days to first harvest and number of seeds per pod. In the present study, the twenty nine genotypes could be grouped into fourteen clusters based on genetic distance. High coefficient of variation was observed for pod yield per plant, pod weight, number of pods per plant and pod length indicating their significant contribution in determining the inter cluster distances. Key words: Correlation coefficient, Genetic divergence, Quantitative character, Vegetable cowpea INTRODUCTION Vegetable cowpea popularly known as Yard long bean (Vigna unguiculata va r. sesquipedalis) is a n important leguminous vegetable crop of South India. Vegetable cowpea is an important vegetable grown as intercrop in different cropping systems. (Khanpara et al., 2016). In Vegetable cowpea, a mong the different parts analyzed shells were rich in dietary fiber. Seeds were nutrient dense as compared to pods and shells, but more in anti- nutrients (Tiwari et al., 2019). In Goa, pole type cowpea with indeterminate growth habit producing long green fleshy pods are preferred and fetch premium price in the market through out the year. There are many varieties released in case of bush type of cowpea but the availa bility of impr oved va rieties in pole type vegetable cowpea is rather scanty. Not much work has been carried out on the genetic improvement of pole type vegetable cowpea. There is wide variability found for different morphological and other traits in the loca l types cultivated in the state of Goa . Exploration of genetic variability in the available germplasm is a prerequisite for initiation of any successful br eeding programme. In spite of its popularity and importance very little effort has been This is an open access article d istributed under the terms of Creative Commons Attribution-NonCommer cial-ShareAl ike 4.0 International License, which permits unrestricted non-commercial use, d istribution, and reproduction in any med ium, provide d the original author and source are credited. 46 J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 Thangam et al. made to upgrade the genetic makeup of this crop. Hence, the present investigation was carried out systematically to evaluate the local accessions to estimate the extent of genetic variability, heritability, genetic advance and genetic divergence in the locally collected germplasm of vegetable cowpea. MATERIALS AND METHODS Twenty nine genotypes of vegetable cowpea collected from different parts of Goa including three released varieties were evaluated in a randomized block design with two replications during rabi seasons starting from 2012-2016 in paddy fallow land. The soil is sandy loam with a pH of 5.1 with medium phosphorous and potassium availability. Recommended package of practices were followed to raise a good crop (Anon. 2004). Obser vations wer e r ecor ded on twelve important quantitative characters, viz., plant height (cm), number of primary branches, leaf length (cm), leaf width (cm), days to first flowering, days to first harvest, pod length (cm), pod weight (g), pods per plant, number of seeds per pod, 100 seeds weight (g) and pod yield per plant on five randomly selected plants/genotype/replication. The analysis of variance was carried out as suggested by Panse and Sukhatme (1985). The genotypic and phenotypic coefficient of variation was calculated as per the formula suggested by Comstock and Robinson (1952). Heritability (broad sense) and genetic advance were worked out as per the procedure given by Burton and De Vane (1953) and Allard (1960). RESULTS AND DISCUSSION Among the twelve quantitative characters studied, the twenty nine vegetable cowpea genotypes exhibited highly significant differences for all the characters indicating high variability in the cowpea accessions (Table 1). Wide range of variation was observed for all the characters studied. Highest variation was observed for pod yield/plant, number of pods/plant and pod length (Table 2). Such a high variability for the above characters was also reported earlier by De Mooy (1985), Resmi (1998) and Narayanankutty et al. (2003). In the present study, all the characters exhibited narrow differences between the value of PCV and GCV. This indicated low impact of environment on the expression of all the quantitative characters. The same was reported earlier by Narayanankutty et al. (2005). The GCV value was maximum for pod yield per plant (g) followed by pod weight (g) and number of pods per plant. Low values of GCV were observed for days to first flowering, days to first harvest and number of seeds per pod (Table 2). Shobha and Vahab (1998) and Narayanankutty et al., (2003) reported high GCV for yield per plant and pod weight in vegetable cowpea. Low GCV values for days to first flowering and number of seeds per pod has been reported by Sreekumar et al., (1996). The results of analysis of variance for different traits are given in Table 3. The high variability for pod yield per plant is apparent as the pod yield ranged from 315.25 to 2070.45 g / plant with an average of 827.48 g per plant. Pod yield depends on number of pods per plant, pod length and pod weight. Number of pods per plant ranged from 36.65 to 147.80. Pod weight depends on pod length, number of seeds per pod and hundred seeds weight. Wide variation was observed for all these characters in the present study. Similar findings have been reported by other workers (De Mooy(1985), Resmi (1998), Shobha and Vahab(1998) and Narayanankutty et al.,(2005) With the help of variability and subsequent GCV alone, it is not possible to determine the amount of genetic variation that is heritable to the further generations. Burton and De Vane (1953) suggested that GCV combined with estimates of heritability would give the best results of genetic advance to be expected from selection. In the present study, heritability values were high (>90%) for all the characters studied except number of seeds per pod. High values of heritability for quantitative characters have also been reported by earlier workers, Sobha and Vahab (1998) and Narayanankutty et al. (2003). The accurate value for heritable variation can be estimated when heritability is combined with genetic advance. In the present study, high heritability coupled with high genetic advance was observed for pod yield per plant (g) and pod weight (g). This may be due to the preponderance of additive gene action for these characters there by indicating the advantages of selection for their 47 Variability and genetic divergence in vegetable cowpea germplasm of Goa J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 S .N o. A cc es si o n P la n t N o of L ea f L ea f D ay s to D ay s to P od P od P o d s N o of 10 0 P od n u m b er h ei g h t 1o le n g th w id th 1s t 1s t le n g th w ei g h t p er se ed s p er s ee d s yi el d p er b ra n ch es fl o w er in g h ar ve st p la n t po d w ei g h t p la n t 1 V C G 1 4. 49 6. 60 13 .4 8 9. 68 43 .0 0 57 .6 0 35 .1 7 10 .5 8 77 .0 0 14 .6 1 17 .3 2 82 0. 50 2 V C G 2 5. 29 8. 25 11 .3 9 8. 25 50 .4 0 71 .1 5 40 .7 7 12 .3 6 77 .6 5 14 .7 0 27 .2 3 95 0. 50 3 V C G 3 4. 85 5. 25 10 .6 3 7. 77 51 .3 0 71 .0 0 47 .3 5 13 .9 0 74 .4 0 13 .7 6 25 .6 5 10 30 .4 5 4 V C G 4 6. 29 4. 10 12 .5 4 9. 56 46 .4 0 60 .0 0 64 .7 0 35 .5 2 58 .3 5 13 .1 9 19 .2 2 20 70 .4 5 5 V C G 5 3. 46 5. 60 9. 98 6. 47 52 .2 0 69 .7 0 40 .8 5 9. 26 54 .5 0 17 .3 4 25 .2 5 50 5. 50 6 V C G 6 4. 37 5. 85 11 .2 9 8. 67 56 .1 5 69 .9 0 39 .4 2 9. 00 50 .4 5 16 .9 7 19 .8 2 45 5. 40 7 V C G 7 3. 90 5. 25 11 .1 3 9. 33 48 .5 0 62 .9 0 37 .5 3 8. 47 67 .1 5 15 .1 5 14 .9 0 56 5. 65 8 V C G 8 4. 26 4. 60 12 .2 1 9. 14 43 .5 0 60 .0 0 37 .9 0 11 .5 8 14 7. 80 15 .6 3 18 .4 7 17 10 .6 5 9 V C G 9 4. 35 4. 85 11 .9 4 9. 34 45 .2 0 60 .9 0 36 .6 9 9. 80 92 .4 5 14 .5 2 22 .5 0 91 0. 40 1 0 V C G 10 4. 41 6. 65 10 .8 4 7. 97 50 .6 0 66 .9 0 40 .3 2 11 .1 3 72 .5 0 14 .7 7 13 .4 5 81 0. 00 1 1 V C G 11 4. 99 4. 40 11 .8 8 7. 40 46 .4 0 64 .2 0 41 .2 4 10 .8 9 78 .5 0 15 .8 8 20 .1 1 85 0. 45 1 2 V C G 12 4. 64 4. 80 11 .0 8 7. 27 51 .9 0 77 .1 0 43 .0 1 9. 60 58 .4 0 16 .5 5 15 .3 9 55 5. 40 1 3 V C G 13 3. 72 5. 05 11 .7 8 8. 29 54 .5 0 74 .3 0 40 .5 5 12 .8 4 51 .5 0 17 .9 1 20 .3 1 66 0. 25 1 4 V C G 14 4. 31 4. 15 13 .7 3 9. 93 44 .2 0 61 .2 0 41 .1 9 9. 74 65 .0 5 15 .5 1 20 .5 1 63 5. 25 1 5 V C G 15 4. 38 5. 00 12 .7 8 10 .6 6 43 .6 0 60 .0 0 39 .0 2 10 .6 8 85 .3 5 15 .9 7 19 .2 9 91 0. 45 1 6 V C G 16 4. 75 4. 85 12 .2 4 9. 58 45 .3 0 60 .7 0 27 .9 7 6. 22 83 .6 0 15 .2 6 20 .9 3 52 0. 65 1 7 V C G 17 4. 08 4. 70 13 .0 0 9. 80 51 .6 0 62 .8 0 31 .5 4 10 .6 1 59 .1 0 15 .9 9 22 .3 3 62 5. 20 1 8 V C G 18 4. 46 4. 25 13 .6 0 9. 93 55 .2 0 67 .0 0 33 .3 6 8. 66 36 .6 5 16 .1 1 20 .1 1 31 5. 25 1 9 V C G 19 3. 39 5. 05 10 .5 4 10 .3 9 42 .1 0 66 .0 0 46 .1 3 12 .0 8 44 .1 5 15 .7 5 15 .0 4 52 7. 30 2 0 V C G 20 4. 64 4. 85 11 .4 2 9. 05 44 .4 0 71 .9 0 51 .9 5 12 .0 3 52 .2 0 14 .9 7 17 .5 5 60 6. 20 2 1 V C G 21 4. 43 5. 55 11 .8 9 9. 24 44 .0 0 71 .6 0 48 .1 4 11 .1 3 86 .4 5 13 .0 3 20 .0 5 90 8. 45 2 2 V C G 22 4. 95 6. 25 12 .0 7 10 .2 1 46 .1 0 70 .2 0 59 .5 0 19 .1 3 72 .5 0 16 .8 6 22 .2 9 13 74 .1 5 2 3 V C G 23 4. 10 6. 15 11 .7 4 9. 87 44 .0 0 65 .6 0 55 .6 4 10 .5 7 10 5. 50 16 .0 4 20 .3 9 11 83 .5 5 2 4 V C G 24 4. 72 5. 30 11 .1 4 9. 61 51 .8 0 78 .4 0 46 .8 7 13 .3 1 91 .6 0 13 .0 6 23 .5 6 12 62 .4 5 2 5 V C G 25 4. 86 4. 95 12 .3 3 9. 10 51 .0 0 77 .6 0 48 .7 7 14 .0 9 48 .8 0 13 .6 1 20 .6 1 69 7. 50 2 6 V C G 26 3. 95 4. 65 13 .1 3 11 .2 0 48 .7 0 62 .5 0 51 .0 1 13 .9 4 64 .1 0 13 .6 1 19 .8 4 78 3. 10 2 7 V ij ay an th i 2. 90 6. 15 11 .1 3 10 .9 5 50 .7 0 72 .0 0 47 .2 3 10 .7 5 50 .7 0 12 .9 4 23 .2 1 40 8. 60 2 8 A G ar im a 1. 00 5. 20 10 .0 0 10 .8 9 42 .9 0 64 .6 0 51 .4 0 10 .9 5 71 .3 0 12 .2 0 21 .9 6 70 7. 80 2 9 A S um an 1. 05 5. 10 9. 39 10 .0 9 45 .9 0 65 .0 0 54 .2 8 12 .0 7 65 .2 0 13 .0 5 19 .9 4 63 5. 45 C D (0 .0 5) 0. 61 6. 60 0. 82 0. 71 3. 06 3. 98 3. 36 1. 63 7. 36 1. 92 1. 62 12 3. 72 T ab le 1 . M ea n pe rf or m an ce o f ve ge ta bl e co w pe a ge rm pl as m o f G oa 48 Thangam et al. J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 Ta bl e 2. R an ge , m ea n, P C V , G C V, h er it ab ili ty a nd g en et ic a dv an ce f or t w el ve q ua nt it at iv e ch ar ac te rs i n ve ge ta bl e co w pe a C ha ra ct er s M ea n ± SE m R an ge P he no ty pi c G en ot yp ic PC V G C V H er it ab ili ty G en et ic va ri an ce va ri an ce (% ) ad va nc e (% ) Pl an t he ig ht 4. 17 0± 0. 21 0 1. 00 - 6 .2 9 2. 31 2. 22 36 .4 5 35 .7 4 96 .1 9 72 .2 1 N o of 1 o br an ch es 5. 29 ±0 .2 4 4. 10 - 8 .2 5 1. 59 1. 47 23 .8 1 22 .9 5 92 .9 2 45 .5 8 L ea f le ng th 11 .7 3± 0. 29 9. 39 - 1 3. 73 2. 41 2. 25 13 .2 4 12 .7 9 93 .2 8 25 .4 4 L ea f w id th 9. 29 ±0 .2 5 6. 46 - 1 1. 19 2. 69 2. 57 17 .6 4 17 .2 5 95 .5 5 34 .7 3 D ay s to 1 st f lo w er in g 47 .9 8± 1. 06 42 .1 0 - 56 .1 5 33 .8 8 31 .6 4 12 .1 3 11 .7 2 93 .4 0 23 .3 4 D ay s to 1 st h ar ve st 66 .9 9± 1. 38 57 .6 0 - 78 .4 0 67 .7 2 63 .9 5 12 .2 8 11 .9 4 94 .4 3 23 .8 9 Po d le ng th 44 .1 2± 1. 16 27 .9 7 - 64 .6 9 14 2. 48 13 9. 79 27 .0 6 26 .8 0 98 .1 2 54 .6 9 Po d w ei gh t 12 .0 9± 0. 56 6. 22 - 3 5. 52 51 .4 8 50 .8 5 59 .3 1 58 .9 4 98 .7 7 12 0. 67 N o of p od s/ pl an t 70 .4 5± 2. 54 36 .6 5 - 14 7. 80 96 7. 57 95 4. 66 44 .1 7 43 .8 6 98 .6 7 89 .7 5 N o of s ee ds /p od 14 .9 9± 0. 66 12 .2 0 - 17 .9 1 4. 48 3. 60 14 .1 1 12 .6 6 80 .4 3 23 .3 8 10 0 se ed w ei gh t 20 .2 5± 0. 56 13 .4 5 - 27 .2 3 20 .5 0 19 .8 8 22 .3 6 22 .0 2 96 .9 6 44 .6 7 Y ie ld p er p la nt 82 7. 48 ±4 2. 72 31 5. 25 - 2 07 0. 45 30 34 98 .4 5 29 98 49 .3 8 66 .5 8 66 .1 8 98 .7 9 13 5. 49 Ta bl e 3. A na ly si s of V ar ia nc e fo r D if fe re nt t ra it s in V eg et ab le C ow pe a ge rm pl as m o f G oa So ur ce s of d. f. M ea n Su n of S qu ar es V ar ia ti on P la nt N o of L ea f L ea f D ay s to D ay s to Po d Po d P od s N o of 10 0 Po d he ig ht br an ch es le ng th w id th 1s t 1s t le ng th w ei gh t pe r se ed s se ed s yi el d pe r fl ow er in g ha rv es t p la nt pe r po d w ei gh t pl an t T re at m en ts 28 .0 0 64 .6 7* 44 .4 2 67 .5 3 75 .3 1 94 8. 59 * 18 96 .2 4 39 89 .5 5* 14 41 .3 9* 27 09 1. 81 12 5. 41 97 95 6. 69 57 4. 08 * R ep lic at io ns 1. 00 0. 47 0. 08 0. 07 0. 05 0. 03 0. 38 1. 32 0. 65 0. 02 1. 07 13 83 .7 7 0. 21 R es id ua l 28 .0 0 2. 47 3. 15 4. 54 3. 35 62 .5 8 10 5. 70 75 .1 9 17 .7 2 36 1. 41 24 .5 5 21 74 .0 8 17 .4 8 *S ig ni fic an t at 1 % l ev el 49 Variability and genetic divergence in vegetable cowpea germplasm of Goa J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 Ta bl e 4. C lu st er in g pa tt er n of v eg et ab le c ow pe a ge no ty pe s T ra it s P la nt N o of 1 o L ea f L ea f D ay s to D ay s to Po d Po d N o of N o of 10 0 Y ie ld he ig ht br an ch es le ng th w id th 1s t 1s t le ng th w ei gh t po ds / se ed s/ se ed pe r fl ow er in g ha rv es t pl an t po d w ei gh t pl an t Pl an t he ig ht 1. 00 0 -0 .1 76 * 0. 68 8* * 0. 34 5* 0. 15 0* * 0. 06 0 -0 .0 76 0. 36 0* 0. 10 4 0. 23 9 0. 00 8 0. 42 3* N o of 1 o br an ch es 1. 00 0 -0 .3 73 -0 .2 86 0. 06 1 0. 20 1 0. 08 4 -0 .1 30 0. 08 3* -0 .0 93 0. 21 9 -0 .0 46 L ea f le ng th 1. 00 0 0. 82 9 0. 03 3 -0 .1 41 -0 .2 97 0. 04 7 0. 06 4 0. 25 8* * -0 .0 22 0. 13 5 L ea f w id th 1. 00 0 -0 .1 64 -0 .1 57 -0 .0 73 0. 06 3 0. 05 0 -0 .0 22 -0 .0 24 0. 10 9 D ay s to 1 st f lo w er in g 1. 00 0 0. 56 7* * -0 .2 29 -0 .0 80 -0 .4 69 0. 28 0* 0. 24 2* -0 .3 18 * D ay s to 1 st h ar ve st 1. 00 0 0. 23 7* -0 .0 39 -0 .3 26 0. 01 3 0. 22 0* -0 .1 95 Po d le ng th 1. 00 0 0. 70 0* * -0 .0 77 -0 .3 90 ** 0. 05 5 0. 48 9* Po d w ei gh t 1. 00 0 -0 .0 83 * -0 .2 67 0. 04 4 0. 73 7* * N o of p od s/ pl an t 1. 00 0 -0 .0 99 0. 07 2* 0. 59 6* * N o of s ee ds /p od 1. 00 0 -0 .1 39 ** -0 .2 03 * 10 0 se ed w ei gh t 1. 00 0 0. 09 6 * Si gn ifi ca nt a t 5 % l ev el ; ** S ig ni fic an t at 1 % l ev el Ta bl e 5. P he no ty pi c co rr el at io n co ef fi ci en t am on g di ff er en t qu an ti ta ti ve c ha ra ct er s in v eg et ab le c ow pe a C lu st er N o. N um be r of g en ot yp es C on st it ue nt g en ot yp es I 5 V C G 1, V C G 2, V C G 3, V C G 9, V C G 15 II 2 A S um an , A G ar im a II I 2 V C G 6, V C G 13 IV 2 V C G 18 , V C G 17 V 2 V C G 11 , V C G 14 V I 2 V C G 25 , V C G 20 V II 2 V C G 10 , V C G 12 V II I 2 V C G 7, V C G 19 IX 2 V C G 24 , V C G 21 X 2 V ija ya nt hi , V C G 26 X I 2 V C G 23 , V C G 22 X II 2 V C G 5, V C G 16 X II I 1 V C G 4 X IV 1 V C G 8 50 Thangam et al. J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 improvement. High heritability coupled with high genetic advance for above characters in vegetable cowpea has been reported by Resmi (1998) and Narayanankutty et al. (2003). Other characters viz., days to first flowering, days to first harvest, number of seeds per pod and leaf length has recorded high heritability of more than 90 per cent but their genetic advance is very low (<30%) indicating the non additive gene action for these traits. This implies improvement of above traits by pyramiding desirable genes through suitable hybridization programmes. The success of any hybridization programme depends on the genetic diversity present in the parents. In the present study, the twenty nine genotypes could be grouped in to fourteen clusters based on genetic distance (Table 4). The cluster I was the largest comprising of five genotypes and remaining clusters had two genotypes each except thirteen and fourteen that had one genotype each. The clustering pattern in the present study did not follow any uniform pattern. The clustering pattern was irregular and the same type of distribution was earlier r epor ted by Pa til a nd Bha pka r (1987) a nd Narayanankutty et al. (2005). The correlation studies provide reliable information on the nature and extent of relationship for bringing about improvement in yield and other traits. The estimates of correlation coefficients is presented in Table 5. Characters showing positive and highly significant correlation with yield per plant were pod weight (0.737) and number of pods per plant (0.596). On the other hand, yield ha d nega tive and significant correlation with days to first flowering (-0.318) and number of seeds per pod (-0. 203). T his is in accordance with the results of Narayanankutty et al. (2005). In the present study, high coefficient of variation was observed for pod yield per plant, pod weight, number of pods per plant and pod length indicating their significant contribution in determining the inter cluster distances. Hence, selection of parents differing in traits such as pod weight, pod yield per plant , number of pods/plant and pod length will be more useful in future breeding programmes. REFERENCES Allard, R.W. 1960. Principles of Plant Breeding. John Willey and Sons; Inc., New York. pp. 83-108. Anon 2004. Crop varieties developed by DBS Konkan Krishi Vidyapeeth, Dapoli. Directorate of Research, Dr.B.S.Konkan Krishi Vidyapeeth, Dapoli, Ratnagiri (M.S.), India. Burton, G.W and De Vane, E.H. 1953. Estimating her ita bility in ta ll fescue (Festuca arundinaceae) from replicated clonal material. Agron. J. 43: 478-81. Comstock, R.E and Robinson, H.F.1952. Genetic parameters, their estimation and significance. Proc. VI Intl. Grassland Congress. 1: 284-91. De Mooy. 1985. Variability of different characteristics in Botswana cowpea germplasm. Tropical Grain Legume Bull. 31: 1-4. Tiwari D., Dutta A., Singh Y.V., Raghuvanshi R.S., Shukla P. , Kha n R. , Tila r a S. (2019) Physicochemica l a nd or ga noleptic characteristics of different parts of vegetable cowpea [Vigna Unguiculata(L. ) Wa lp]. Indian J.of Agri. Res. 53 (6) : 662-668. Khanpara S.V., Jivani L.L., Vachanni J.H. and Kachchadia H. (2016) Genetic variability, heritability and genetic advance studies in vegetable cowpea [Vigna unguiculata (L.) Walp.]. Electronic Journal of Plant Breeding 7(2) : 408-413 Narayanankutty C., Mill R. and Jaikumaran U. 2003. Variability and genetic divergence in vegetable cowpea. J. Maharashtra Agric. Univ., 28: 26- 29. Narayanankutty C., Sunanda C.K and Jaikumaran U. 2005. Genetic diver gence in pole type vegetable cowpea. Indian J. Hort. 62: 354-57. Panse V.G and Sukhatme P.V. 1985. Statistical Methods for Agricultural Workers. Fourth 51 Variability and genetic divergence in vegetable cowpea germplasm of Goa J. Hortl. Sci. Vol. 15(1) : 45-51, 2020 (Received on 22.11.2019 and accepted on 05.01.2020) Edition, Indian Council of Agricultural Research, New Delhi. Patil R.B and Bhapkar D.G. 1987. Genetic divergence among 49 cowpea strains. J. Maharashtra Agric. Univ. 12: 283-85. Resmi P.S. 1998. Genetic variability in yard long bean (Vigna unguiculata subsp. sesquipedalis (L) Ver dcour t). M. Sc. (Ag. ) thesis, Ker a la Agricultural University, Thrissur, 116p. Shobha P.P. and Abdul Vahab M. 1998. Genetic variability, heritability and genetic advance in cowpea (Vigna unguiculata (L) Walp.). J. Trop. Agric. 36: 21-23. Sreekumar K.K., Inasi K.A., Antony A. and Nair R.R. 1996. Genetic variability, heritability and correlation studies in vegetable cowpea (Vigna unguiculata var. sesquipedalis). South Ind. Hort. 44: 15-18.