Final SPH -JHS Coverpage 16-2 Jan 2021 single C O N T E N T S JOURNAL OF HORTICULTURAL SCIENCES Volume 16 Issue 2 June 2021 In this Issue i-ii Review Phytoremediation of indoor air pollutants: Harnessing the potential of 131-143 plants beyond aesthetics Shalini Jhanji and U.K.Dhatt Research Articles Response of fruit yield and quality to foliar application of micro-nutrients in 144-151 lemon [Citrus limon (L.) Burm.] cv. Assam lemon Sheikh K.H.A., Singh B., Haokip S.W., Shankar K., Debbarma R. Studies on high density planting and nutrient requirement of banana in 152-163 different states of India Debnath Sanjit Bauri F.K., Swain S., Patel A.N., Patel A.R., Shaikh N.B., Bhalerao V.P., Baruah K., Manju P.R., Suma A., Menon R., Gutam S. and P. Patil Mineral nutrient composition in leaf and root tissues of fifteen polyembryonic 164-176 mango genotypes grown under varying levels of salinity Nimbolkar P.K., Kurian R.M., Varalakshmi L.R., Upreti K.K., Laxman R.H. and D. Kalaivanan Optimization of GA3 concentration for improved bunch and berry quality in 177-184 grape cv. Crimson Seedless (Vitis vinifera L) Satisha J., Kumar Sampath P. and Upreti K.K. RGAP molecular marker for resistance against yellow mosaic disease in 185-192 ridge gourd [Luffa acutangula (L.) Roxb.] Kaur M., Varalakshmi B., Kumar M., Lakshmana Reddy D.C., Mahesha B. and Pitchaimuthu M. Genetic divergence study in bitter gourd (Momordica charantia L.) 193-198 Nithinkumar K.R., Kumar J.S.A., Varalakshmi B, Mushrif S.K., Ramachandra R.K. , Prashanth S.J. Combining ability studies to develop superior hybrids in bell pepper 199-205 (Capsicum annuum var. grossum L.) Varsha V., Smaranika Mishra, Lingaiah H.B., Venugopalan R., Rao K.V. Kattegoudar J. and Madhavi Reddy K. SSR marker development in Abelmoschus esculentus (L.) Moench 206-214 using transcriptome sequencing and genetic diversity studies Gayathri M., Pitchaimuthu M. and K.V. Ravishankar Generation mean analysis of important yield traits in Bitter gourd 215-221 (Momordica charantia) Swamini Bhoi, Varalakshmi B., Rao E.S., Pitchaimuthu M. and Hima Bindu K. Influence of phenophase based irrigation and fertigation schedule on vegetative 222-233 performance of chrysanthemum (Dendranthema grandiflora Tzelev.) var. Marigold Vijayakumar S., Sujatha A. Nair, Nair A.K., Laxman R.H. and Kalaivanan D. Performance evaluation of double type tuberose IIHR-4 (IC-0633777) for 234-240 flower yield, quality and biotic stress response Bharathi T.U., Meenakshi Srinivas, Umamaheswari R. and Sonavane, P. Anti-fungal activity of Trichoderma atroviride against Fusarium oxysporum f. sp. 241-250 Lycopersici causing wilt disease of tomato Yogalakshmi S., Thiruvudainambi S., Kalpana K., Thamizh Vendan R. and Oviya R. Seed transmission of bean common mosaic virus-blackeye cowpea mosaic strain 251-260 (BCMV-BlCM) threaten cowpea seed health in the Ashanti and Brong-Ahafo regions of Ghana Adams F.K., Kumar P.L., Kwoseh C., Ogunsanya P., Akromah R. and Tetteh R. Effect of container size and types on the root phenotypic characters of Capsicum 261-270 Raviteja M.S.V., Laxman R.H., Rashmi K., Kannan S., Namratha M.R. and Madhavi Reddy K. Physio-morphological and mechanical properties of chillies for 271-279 mechanical harvesting Yella Swami C., Senthil Kumaran G., Naik R.K., Reddy B.S. and Rathina Kumari A.C. Assessment of soil and water quality status of rose growing areas of 280-286 Rajasthan and Uttar Pradesh in India Varalakshmi LR., Tejaswini P., Rajendiran S. and K.K. Upreti Qualitative and organoleptic evaluation of immature cashew kernels under storage 287-291 Sharon Jacob and Sobhana A. Physical quality of coffee bean (Coffea arabica L.) as affected by harvesting and 292-300 drying methods Chala T., Lamessa K. and Jalata Z Vegetative vigour, yield and field tolerance to leaf rust in four F1 hybrids of 301-308 coffee (Coffea arabica L.) in India Divya K. Das, Shivanna M.B. and Prakash N.S. Limonene extraction from the zest of Citrus sinensis, Citrus limon, Vitis vinifera 309-314 and evaluation of its antimicrobial activity Wani A.K., Singh R., Mir T.G. and Akhtar N. Event Report 315-318 National Horticultural Fair 2021 - A Success Story Dhananjaya M.V., Upreti K.K. and Dinesh M.R. Subject index 319-321 Author index 322-323 J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 This is an open access article d istributed under the terms of Creative Commons Attribution-NonCommer cial-ShareAl ike 4.0 International License, which permits unrestricted non-commercial use, d istribution, and reproduction in any med ium, provide d the original author and source are credited. Original Research Paper Response of fruit yield and quality to foliar application of micro-nutrients in lemon [Citrus limon (L.) Burm.] cv. Assam lemon Sheikh, K.H.A.1*, Singh, B.1, Haokip, S.W.1, Shankar, K.1, Debbarma, R.1, Gaitri Devi, A.2 and Nengparmoi T.H.3 1Department of Fruit Science, 2Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat-791102, Arunachal Pradesh, India 3Department of Agronomy, College of Agriculture, Vellanikkara, Kerala Agricultural University, Thrissur, Kerala - 680656 *Corresponding Author Email : sheikh4261@gmail.com ABSTRACT Assam lemon [Citrus limon (L.) Burm.], an indigenous lemon cultivar of Assam, is widely cultivated in warm southern slopes of the Himalayas in North-Eastern India. Since this cultivar of lemon is having a prominent trait of bearing fruits in several flushes throughout the year, it is essential to provide sufficient nutrition for obtaining optimum yield with good quality fruits. In the current experiment, a randomized block design having twelve treatments with three replications was followed to find out the response of lemon fruit yield and quality to foliar application of micronutrients during the year 2019. Among all, the treatment ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%) gave the best performance in improving the yield and quality of fruits. The highest number of fruits per plant at the time of harvesting (73), yield per plant (11.5 kg), fruit fresh weight (158 g), fruit length (9.60 cm), fruit diameter (5.80 cm), juice content (152 mL/fruit), TSS (6.40 °B), ascorbic acid (49.10 mg/100g), total sugar (6.30%), reducing sugar (3.90%), non-reducing sugar (2.40%) with lowest titratable acidity (3.13%) were obtained which revealed that the yield and fruit quality of lemon depends on the application of different micronutrients. Keywords: Assam lemon, fruit quality, micronutrients, nutrition and yield INTRODUCTION Citrus is considered as one of the most important fruits widely cultivated in different parts of the world. It belongs to the family Rutaceae. It is very famous for its juice and pulp all over the world. Among the citrus cultivar, Assam lemon [Citrus limon (L.) Burm.] is an indigenous lemon cultivar of Assam and is grown all over the North-Eastern region. As this plant is having a prominent trait of bearing fruits in several flushes throughout the year, it is essential to provide sufficient nutrition for obtaining optimum yield with good quality fruits. Micronutrients like zinc, iron, boron and copper are not only essential but they are equally significant like other macronutrients, in spite of their requirement in minute quantities. They also play a vital role in the various enzymatic activities and synthesis. Their acute deficiencies are sometimes incurable in nature (Kumar, 2002). Zinc is required for the synthesis of tryptophan which is the precursor of indole acetic acid synthesis resulting in the growth and development of tissues. Iron is also an important micronutrient necessary for the citrus plants. It has been reported that the application of iron sulphates as folia r spr a y r educes the lea f chlor osis a nd consequently increase the yield (Devi et al., 1997). It also helps in a significant increase of fruit yield, fruit volume, ascorbic acid content and leaf iron content in citrus (Aboutalebi and Hassanzadeh, 2013). Similarly, boron also plays a vital role in the growth behaviour and productivity of citrus fruits. It increases the phenolic compound production in the plant system that is responsible for the polar transport of auxin. This increases the auxin activity resulting in increasing the vegetative growth of citrus trees (Gurjar et al., 2015). Boron also helps in pollen grain germination and 145 Response of fruit yield and quality to foliar application of micro-nutrients in lemon J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 elongation of pollen tubes that results in increasing the fruit set percentage and ultimately increase in yield (Abd-Allah, 2006). As a micronutrient, copper plays a significant role in plants. It is involved in stimulation activities for lignification of the cell wall of plants, and photosynthesis, and acts as an electron carrier in the plant system (Somasundaram et al., 2011). It is reported that copper helps in production of sugar compounds and leads to more accumulation of total soluble solids (TSS) in the fruit juice (Singh et al., 2018). Since citrus is a micronutrient loving crop, the more precise management of nutrition is required to meet the nutrient demand for its growth and development. Thus, fulfilling the nutritional requirement is very important for economically profitable citrus fruit production. Assam lemon being a heavy and regular bearing crop which bears throughout the year has to be supplied with adequate nutrients to ensure the yield and quality of the harvest. Most of the time citrus growers are not giving proper emphasis to application of micronutrients, as they are required in minute quantities. This leads to a drastic decrease in the growth and development of plants and it also affects the yield a nd quality of fruits. So, the curr ent experiment was conducted to study the effect of micronutrients on fruit yield and quality of Assam lemon in the North-Eastern region of India. MATERIAL AND METHODS The experiment was laid out with the objectives of eva lua ting a nd sta nda r dizing the impa ct of micronutrients on yield and quality of Assam lemon. The research work was executed on three years old Assam lemon plants which were planted at the spacing of 3m x 3m during the year 2019 at the Citrus Fruit Block, Department of Fruit Science of the College of Horticulture and Forestry, Central Agricultural University, Pa sigha t, Aruna cha l Pr adesh. It is geographically located at 28° 04’ 43" N latitude and 95° 19’ 26" E longitude with an altitude of 153 m above the mean sea level. Pasighat lies under the humid sub-tropical climate. The average annual rainfall and temperature of this area are 32.8 cm and 25.5 ºC, respectively. The rainy season starts from June and it continues till September with maximum rainfall during July. August is the warmest month of the year while December is the coldest month of the year with the average temperature of 31.60C and 20.50C P C respectively. The design of the experiment followed was Randomized Block Design (RBD) having twelve treatments and each treatment was replicated thrice. In each replication, there were two plants and the total numbers of plants in the whole experiment were seventy-two. The recommended dose of fertilizer (RDF) for Assam lemon 100: 100: 100 g NPK (Source: N- Urea, P- SSP and K- MOP)/plant/ year (www.kiran.nic.in), was applied to all the plants under the investigation. Half of the dose before flowering during the first week of January and the remaining half dose during the second month of June were applied. The treatments studied were: T1 - Control, T2 - ZnSO4 (0.2%), T3 - FeSO4 (0.2%), T4 - Borax (0.2%), T5 - CuSO4 (0.2%), T6 - ZnSO4 (0.2%) + FeSO4 (0.2%), T7 - ZnSO4 (0.2%) + Borax (0.2%), T8 - ZnSO4 (0.2%) + CuSO4 (0.2%), T9 - FeSO4 (0.2%) + Borax (0.2%), T10 - FeSO4 (0.2%) + CuSO4 (0.2%), T11 - Borax (0.2%) + CuSO4 (0.2%) and T12 - ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%). For the foliar application of these micronutrients (Sources: ZnSO4- 36.40% Zn, FeSO4- 32.8% Fe, Borax- 10.8% B and CuSO4- 21% Cu), the required amounts of micronutrient sources were dissolved in separate container. Then the pH of the nutrient solutions were checked by using Pen type digital pH meter and it was adjusted by 0.1 N concentrated hydrochloric acid and sodium hydroxide. The application was done on 5th April, 2019 (i.e.) after the complete emergence of spring flush and the onset of fruit setting) on an average of two liters per tree and normal water was used for spraying the plants in control by using Knapsack sprayer. In each spray treatment teepol @ 0.01% was added as sticking gent in prepared solution. The total numbers of fruits per plant at the time of harvesting (i.e.) during June-July fruits developed attractive green to little yellow colour and they were harvested two times) were recorded. For fruit yield of Assam lemon, fruits from each plant were harvested separately for all treatments and yield per plant was calculated by multiplying total number of fruits per plant with average fruit weight. For recording the readings of physical parameters like fruit weight, fruit length, fruit diameter and juice content, five fruits from each treatment were selected randomly. Then the fruit weight was recorded by using a precision weighing balance and its average weight was expressed in grams 146 Sheikh et al (g). For the fruit length and fruit diameter a digital Vernier caliper was used to record the data and their average fruit length and fruit diameter were expressed in centimeters (cm). Fruit juice content was also measured by using a measuring cylinder and its average juice content per fruit was expressed in milliliters (mL). The TSS in fruit juice was determined using hand held Refractometer (0 ºB–32 ºB). The titratable acidity of the fruit was determined by titrating the fruit juice against 0.1N NaOH solution using phenolphthalein as an indicator (light pink end point) and expressed as percentage in terms of citric acid (AOAC, 2002). Total sugar content was estimated by anthrone method as described by Hodge and Hofreiter (1962). Reducing suga r content wa s estima ted by spectrophotometric method as described by Somogyi (1952). The non-reducing sugar content was obtained by the subtraction of the reducing sugar content from the total sugar content. Non reducing sugars content = total sugar - reducing sugar The ascorbic acid content of fruits was determined by the method described by Ranganna (1986) using 2, 6- dichlorophenol Indophenol dye. The samples extracted in meta-phosphoric acid solution were titrated with dye to pink end point. The ascorbic acid content was calculated and expressed as mg per 100 g of fruit weight sample. The observations recorded during field experiment and data obtained from laboratory analysis were subjected to the statistical analysis of variance for RBD. Significance and non-significance of the va r ia nc e du e t o diff er ent t r ea t ment s wer e determined by calculating the respective ‘F’ values according to the method described by Gomez and Gomez (2010). RESULTS AND DISCUSSION Yield and its attributing parameters: The data depicted in Table 1 exhibited the significant impact of foliar imposition of micronutrients on yield and its attributing parameters. The highest number of fruits per plant (73) at the time of harvesting and fruit yield (11.52 kg/plant) was recorded in T 1 2 [ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%)] while the lowest number of fruits per plant (43) at the time of harvesting and fruit yield (5.60 kg/plant) was obtained in T1 (Control). This might be due to the synergistic effect of different micronutrients as they directly take part in many physiological processes and activity of many enzymes for greater gathering of food materials. Zinc helps in prevention of abscission layer formation and increase the synthesis of tryptophan which is the precursor of auxin synthesis and this facilitates the ovary to remain intact with the shoot, ensuring in minimizing the flower and fruit drop and maximize the retention of fruits in the plants (Gurjar et al., 2015). There is also a correlation between fruit drop and internal hormonal level in the plant system. As the level of internal auxin concentration in the plant system is higher, then the fruit retention capacity will be more leading to increase in number of fruits per plant. Iron also plays a major role in cell division and cell enlargement resulting in increasing the fruit size and fruit weight which ultimately leads to the increase in yield of plants. Ganie et al. (2013) reported that boron helps in germination of pollen grains and elongation of pollen tube because of which the fruit set, fruit retention and yield of the guava plants were increased. Copper involves in synthesis and stability of chlorophyll responsible to produce food materials required for the growth and development of fruits. Ilyas et al. (2015) observed the significant improvement of photosynthetic and fruit yield in Citrus reticulata Blanco var. Kinnow on the foliar imposition of Zn, Cu and B. Similarly, Zoremtluangi et al., (2019) also proved that the foliar application of Zn, Cu and B obtained the maximum number of fruits per plant and yield per tree. The present report is in conformity with the experimental findings revealed by Bhoyar and Ramdevputra (2016) in guava and Jangid et al. (2019) in aonla. Physical parameters of fruits: Most of the physical parameters of the Assam lemon fruit had consequential effect due to the application of different micronutrients and they are presented in Table 1. The highest fruit fresh weight (157.77 g), juice content (152 mL/fruit), fruit length (9.60 cm) and fruit diameter (5.80 cm) were obtained in treatment T12[ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%)] while the lowest fruit fresh weight (130.20 g), juice content (120 mL/fruit), fruit length (7.43 cm) and fruit diameter (4.10 cm) were obtained in treatment T1 (Control). The overall amelioration in the physical parameters of the Assa m lemon fruit might be because of benefaction of different micronutrients in the growth and development of fruits. Zinc facilitates in the J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 147 Table 1. Effect of micronutrients on yield and physical parameters of Assam lemon Treatments Number of Fruit Fruit Fruit Fruit Juice fruits/plant yield Fresh length diameter content (kg/plant) weight (g) (cm) (cm) (mL/fruit) T1 43 5.60 130.2 7.43 4.10 120 T2 58 8.40 144.60 8.57 5.00 135 T3 48 6.64 138.40 7.87 4.67 129 T4 54 7.68 142.10 8.33 4.90 134 T5 45 6.00 133.30 7.60 4.27 123 T6 70 10.84 155.00 9.43 5.73 149 T7 67 10.11 150.80 9.27 5.50 145 T8 63 9.34 148.13 8.90 5.33 141 T9 52 7.29 140.23 8.13 4.73 131 T10 47 6.38 135.70 7.77 4.43 127 T11 61 8.95 146.60 8.73 5.13 137 T12 73 11.52 157.77 9.60 5.80 152 C.D. (0.05) 7.22 1.17 5.02 0.85 0.54 4.01 SEm± 2.44 0.39 1.70 0.28 0.18 1.36 synthesis of tryptophan, the precursor of auxin synthesis and consequently the auxin level in the fruit increases. This lead to the higher enlargement of cell because of cell vacuolization resulting in increased size of vesicles, dimension of locules and eventually the weight and size of fruit. Therefore, the significant influence of Zn in increasing the fruit fresh weight was revealed by Ghosh and Besra (2000) in sweet orange. Iron and zinc also play a vital role in the enlargement of cell, division of cell and formation of starch. Thus, the additive effect of these micronutrients resulted in increased fruit fresh weight. The rise in fruit fresh weight might be because of the higher translocation of photosynthates to fruits. Similar findings were revealed by Waskela et al. (2013) in guava. Boron also plays a vital role in cell division and cell elongation, thereby increasing the fresh weight of fruit. The increase in fruit fresh weight owing to the combined imposition of Zn and B may be the result of stimulation influence on plant metabolic process. In addition to this, B also activates the sugar and water mobilization in the fruits resulted in increasing the fruit weight as reported by Lakshmipathi et al. (2015). Similarly, Ilyas et al. (2015) also reported that the foliar imposition of Zn, Cu and B had significant effect on fruit yield with regard to number and fresh weight of fruit. Trivedi et al. (2012) reported that zinc controls the semi-permeability of cell wall through which the movement of water into fruits increases, that results in obtaining the highest juice content in guava. Sajid et al. (2012) also revealed that the foliar spray of Zn and B had consequential influence on juice content in fr uits of sweet or a nge. T he imposition of micronutrients might have ameliorated the plant health by improving the sugar metabolism and conduction of assimilates as a result of which the fruit juice content increase. Similar outcomes were revealed by the findings of Singh et al. (2018) in sweet orange cv. Mosambi. Increase in fruit length might be because Response of fruit yield and quality to foliar application of micro-nutrients in lemon J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 148 of the direct effect of B and Zn in increasing the cell division and cell elongation process. Similarly, the fruit diameter was notably influenced by the imposition of zinc sulphate and borax. Dutta and Banik (2005) reported that the increase in fruit length and fruit diameter may be due to the improvement in internal physiology of developing fruit with regard to proper supply of wa ter, miner a l nutr ients a nd other compounds essentia l for nor ma l gr owth a nd development of fruit and the present result is in conformity with the reports of Yadav et al. (2013). Quality parameters of fruit: The quality parameters of fruits were significantly improved by the foliar application of micronutrients and they are presented in Table 2. In this, the treatment T12[ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%)] offered maximum TSS (6.40 ºB), ascorbic acid (49.10 mg/100 g), total sugars (6.30%), reducing sugars (3.90%) and non-reducing sugars (2.40%) content of fruits while the minimum TSS (5.30 ºB), ascorbic acid (34.37 mg/100 g), total sugars (3.73%), reducing sugars (2.53%) and non-reducing sugars (1.20%) were obtained in T1 (Control). Table 2. Effect of micronutrients on quality parameters of Assam lemon Treatments TSS Ascorbic Titratable Total Reducing Non reducing (°Brix) acid acidity sugar sugar sugar (mg/100g) (%) (%) (%) (%) T1 5.30 34.37 4.93 3.73 2.53 1.20 T2 5.93 40.43 4.57 4.80 2.90 1.90 T3 5.50 35.27 4.63 5.13 3.17 1.97 T4 5.60 39.55 4.47 4.37 2.70 1.67 T5 5.83 37.09 4.33 4.63 2.83 1.80 T6 6.13 42.33 3.90 6.00 3.80 1.20 T7 6.00 46.83 3.73 5.53 3.43 2.10 T8 6.20 48.30 3.57 5.73 3.70 2.03 T9 5.90 45.13 3.47 5.67 3.57 2.10 T10 5.97 41.06 3.23 5.37 3.13 2.23 T11 5.80 47.70 3.67 5.47 3.27 2.20 T12 6.40 49.10 3.13 6.30 3.90 2.40 C.D. (0.05) 0.44 3.31 0.39 0.27 0.24 0.40 SEm± 0.15 1.12 0.13 0.09 0.08 0.13 Zinc is required in enzymatic reactions namely hexokinase, carbohydrate and protein synthesis. In addition to this, Boron helps in the transportation of sugar in the form of boron-sugar complex and it also intensifies hydrolysis of carbohydrates into simple suga r. Copper a lso helps in eleva ting the photosynthetic efficiency that results in higher rate of photosynthesis. The main product of photosynthesis is sugar, thus increase in the photosynthesis by the additive action of zinc, boron and copper results in mor e suga r compounds a nd this led to the accumulation of more total soluble solids in fruit juice. The results of the current investigation are in line with the results obtained by Babu and Yadav (2005) in Khasi mandarin and Singh et al. (2018) in Sweet orange cv. Mosambi. Kumari et al. (2009) also Sheikh et al J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 149 reported that the foliar fertilization of Fe, Zn and Cu had significant effect in increasing the TSS of Kinnow mandarin. Zinc helps in the synthesis of auxin that leads to the increase in ascorbic acid content as reported by Nawaz et al. (2008). Similarly, foliar imposition of Zn, B and Cu also helps in increasing the ascorbic acid content in the fruit juice of Sweet orange cv. Mosambi as reported by Singh et al. (2018). Increase in sugar content (total sugar, reducing sugar and non-reducing sugar) in the fruit juice of Assam lemon might be because of the active participation of Zn, Cu and B in photosynthesis and faster translocation of sugars from the site of synthesis to the developing fruits. The other reason might be due to the decrease in starch content during deterioration of acid and quick transfer of sugars in the fruit. Thus, the present findings are in line with the outcomes obtained by El-Rahman (2003) in Naval Orange and Bhatt et al. (2012) in mango. Although, the lowest value of titratable acidity (3.13%) of fruit juice was observed in T12[ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%)] while the highest titratable acidity (4.93%) was recor ded in T 1 (Control). The reduction of titratable acidity might be because of more synthesis of nucleic acids due to more availability of plant metabolites as reported by Ullah et al. (2012) in Kinnow mandarin. The other reason might be because of their utiliza tion in r espir a tion a nd quick transformation of organic acid into sugars as disclosed by Brahmachari et al. (1997) in litchi. Similarly, the outcomes are in agreement with the results obtained by Sau et al. (2018) in guava. On the basis of the experimental evidence obtained from the current research work, it is concluded that the imposition of recommended dose of N, P and K fertilizers (100g: 100g: 100g NPK/plant/year) together with foliar spray of ZnSO4 (0.2%) + FeSO4 (0.2%) + Borax (0.2%) + CuSO4 (0.2%) once (two weeks after fruit setting) can be advocated to Assam lemon growers as the most potent measures to enhance the number of fruits per plant, yield per plant and fruit quality that will eventually increase the productivity. ACKNOWLEDGEMENT The authors would like to express the heartfelt and sincere thanks to the Department of Fruit Science, College of Hor ticulture a nd For estr y, Centr a l Agricultural University, Pasighat, Arunachal Pradesh, for the guidance and needful help during the entire course of the experiment. REFERENCES A.O. A. C. 2002. Officia l methods of ana lysis. Association of Official Analytical Chemists International, Washington D.C. pp. 1-12. Abd-Allah, A.S. 2006. Effect of spraying some macro and micro nutrients on fruit set, yield and fruit quality of Washington Navel orange trees. J. Appl. Sci. Res., 2: 1059-1063. Aboutalebi, A. and Hassanzadeh, H. 2013. Effects of iron and zinc on Sweet lime (Citrus limmetta) fr uit qua ntity a nd qua lity in ca lca r eous soils. Int. J. Farming Allied Sci., 2(18): 675- 677. Babu, K.D. and Yadav, D.S. 2005. Foliar spray of micr onutr ients for yield a nd qua lity impr ovement in Kha si ma nda r in ( Citrus reticulata Blanco.). Indian J. Hortic., 62(3): 280-281. Bhatt, A., Mishra, N.K., Mishra, D.S. and Singh, C.P. 2012. Foliar application of potassium, calcium, zinc and boron enhanced yield, quality and shelf life of mango. Hort Flora Res. Spectrum, 1(4): 300-305. Bhoyar, M.G. and Ramdevputra, M.V. 2016. Effect of foliar spray of zinc, iron and boron on the growth, yield and sensory characters of guava (Psidium guajava L.) cv. Sardar L-49. J. Appl. Nat. Sci., 8(2): 701-704. Brahmachari, V.S., Yadav, G.S. and Naresh, K. 1997. Effect of feeding of calcium, zinc and boron on yield and quality attributes of litchi (Litchi chinensis Sonn.). Orissa J. Hortic., 25(1): 49- 52. Devi, D.D., Srinivasan, P.S. and Balkrishnan, K. 1997. Influence of Zn, Fe a nd Mn on photosynthesis and yield of Citrus sinensis. Indian J. Plant Physiol., 2(2): 174-176. Dutta, P., and Banik, A.K. 2005. Effect of foliar feeding of nutrients and plant growth regulators on physico-chemical quality of Sardar guava grown in red and lateritic tract of West Bengal. Response of fruit yield and quality to foliar application of micro-nutrients in lemon J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 150 In International Guava Symposium. pp. 407- 411, December 5, 2005. Lucknow, India. El-Rahman, A.M.A. 2003. Effects of some nutrients and growth substances application on fruiting, yield and fruit quality of Navel orange trees. Bulletin-Faculty of Agriculture, University of Cairo, 54(2): 175-188. Ganie, M.A., Akhter, F., Bhat, M.A., Malik, A.R., Junaid, J.M. and Shah, M.A. 2013. Boron – a critical nutrient element for plant growth and productivity with reference to temperate fruits. Curr. Sci., 104: 76–85. Ghosh, S.N. and Besra, K.C. 2000. Effect of zinc, boron and iron spray on yield and fruit quality of sweet orange cv. Mosambi grown under rainfed laterite soil. Indian Agric., 44(3/4): 147-51. Gomez, A.K. and Gomez, A.A. 2010. Statistical procedures for agricultural research. 2ndedn. Wiley India Private Limited, New Delhi, pp. 134-138 Gurjar, M.K.,Kaushik, R.A. and Baraily, P. 2015. Effect of zinc and boron on the growth and yield of kinnow mandarin. Int. J. Sci. Res., 4(4): 2277-8179. Hodge, J.E. and Hofreiter, B.T. 1962. Determination of reducing sugars and charbohydrates, In: Whistler, R.L. a nd Wolfr om, M.L. (eds) Methods in carbohydrates chemistry, 17thedn. Academic Press, New York. ICAR Research Complex for NEH Region. Assam Lemon. http://www.kiran.nic.in/pdf/ASSAM LEMON. Accessed 23rd November, 2020. Ilyas, A., Ashraf, M.Y., Hussain, M., Ashraf, M., Ahmed, R. and Kamal, A. 2015. Effect of micr onutr ients (Zn, Cu a nd B) on photosynthetic and fruit yield attributes of Citrus reticulata Blanco var. Kinnow. Pak. J. Bot., 47(4): 1241-1247. Jangid, G. , Mandal, G. and Kumar i, U. 2019. Response of zinc and boron sprays on growth and yield of Aonla (Emblica officinalis Gaertn) cv. Chakiya. J. Pharmacogn. Phytochem., 8(2): 1290-1292. Kumar, P. 2002. Managing micronutrient deficiency in ornamental crops. Indian Hortic., 46(4): 30-31. Kumari, M., Yadav, P.K., Singh, R.S. and Sharma, B. D. 2009. Effect of folia r spr a ys of micronutrients on nutrients status, yield and fruit quality of kinnow mandarin. Haryana J. Hortic. Sci., 38(3&4): 204-206. Lakshmipathi, Dinakara, J., Adiga, Kalaivanan, D., Mohana, G.S. and Meena R.K. 2015. Effect of folia r a pplica tion of micr onutr ients on reproductive growth of cashew (Anacardium occidentale L.) under South West Coast Region of Karnataka, India.Trends in Biosci., 8(2): 447-449. Nawaz, M.A., Ahmad, W., Ahmad, S., and Khan, M.M. 2008. Role of growth regulators on preharvest fruit drop, yield and quality in Kinnow mandarin. Pak. J. Bot., 40: 1971-1981. Ranganna, S. 1986. Handbook of Analysis and Qua lity Contr ol for Fr uit a nd Vegeta ble Products. Tata McGraw Hill Publishing Co. Ltd., New Delhi, pp.190-210. Sajid, M., Rab, A., Jan, I., Haq, I., Shah, S.T., Iqbal, A., Zamin, M. and Shakur, M. 2012. Pre- harvest treatment of Zn & B affects the fruit quality and storability of sweet orange. J. Agric. Sci. Tech., 2: 1224-1233. Sau, S., Sarkar, S., Ghosh, B., Ray, K., Deb, P. and Ghosh, D. 2018. Effect of foliar application of B, Zn and Cu on yield, quality and economics of rainy season guava cultivation. Curr. J. Appl. Sci. and Tech., 28(1): 1-10. Singh, Y., Bhatnagar, P., Meena, N.K. and Gurjar, S.C. 2018. The effect of foliar spray of Zn, Cu and B on physico-chemical parameters of sweet orange (Citrus sinensis L.) cv. Mosambi. J. Pharmacogn. Phytochem., 7(6): 1606-1610. Somasundaram, J., Meena, H.R., Singh, R.K., Prasad, S.N., and Parandiyal, A.K. 2011. Diagnosis of micronutrient imbalance in lime crop in semi- a r id r egion of Ra ja stha n, India . Communications in Soil Sci. Plant Analysis, 42(7): 858-869. Sheikh et al J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 151 Somogyi, N. 1952. Estima tion of suga r s by calorimetric method. J. Biol. Chem., 200: 245. Trivedi, N., Singh, D., Bahadur, V., Prasad, V.M. and Collis, J.P. 2012. Effect of foliar application of zinc and boron on yield and fruit quality of guava (Psidium guajava L.). Hort Flora. Res. Spectrum, 1(3): 281-283. Ullah, S., Khan. A.S., Malik, A.U., Afzal, I., Shahid, S. and Razzaq, K. 2012. Foliar application of boron influences the lea f miner al sta tus, vegetative and reproductive growth, and yield and fruit quality of ‘Kinnow’ mandarin (Citrus reticulate Blanco.). J. Plant Nutr., 35: 2067- 2079. (Received on 14.04.2021, Revised on 13.09.2021 and Accepted on 26.10.2021) Waskela, R.S., Kanpure, R.N., Kumawat, B.R. and Kachouli, B.K. 2013. Effect of foliar spray of micronutrients on growth, yield and quality of guava (Psidium guajava L.) cv. Dharidar. Int. J. Agric. Sci., 9(2): 551-556. Yadav, V., Singh, P.N. and Yadav, P. 2013. Effect of foliar fertilization of boron, zinc and iron on fruit growth and yield of low-chill peach cv. Sharbati. Int. J. Sci. Res. Publ., 3(8): 2250- 2256. Zoremtluangi, J., Saipari, E. and Mandal, D. 2019. Foliar application of zinc, manganese, copper a nd bor on influenced the fr uit gr owth, development and quality of Khasi Mandarin (Citrus reticulata Blanco). J. Pharmacogn. Phytochem., 8(3): 3324-3327. Response of fruit yield and quality to foliar application of micro-nutrients in lemon J. Hortl. Sci. Vol. 16(2) : 144-151, 2021 00 Contents.pdf 02 Sheikh.pdf 19 Lamesssa.pdf 20 Divya.pdf 21 Wani.pdf 23 Index and Last Pages.pdf