Final SPH -JHS Coverpage 16-2 Jan 2021 single 287 J. Hortl. Sci. Vol. 16(2) : 287-291, 2021 This is an open access article d istributed under the terms of Creative Commons Attribution-NonCommer cial-ShareAl ike 4.0 International License, which permits unrestricted non-commercial use, d istribution, and reproduction in any med ium, provide d the original author and source are credited. Original Research Paper Qualitative and organoleptic evaluation of immature cashew kernels under storage Sharon Jacob1 and Sobhana A.2 1Department of Post Harvest Technology, College of Agriculture, Vellanikkara, Kerala Agricultural University, Thrissur - 680656, Kerala, India 2Professor and Head, Fruit Crops Research Station, Thrissur, India *Corresponding author Email : jacobsharon1930@gmail.com ABSTRACT Cashew cultivars, based on flowering behaviour, are categorized into three types, viz., early season, mid-season and late season. In late season type, the harvesting of cashew nuts coincides with the rainy season during which the quality of matured nuts are affected by increased pest and disease attack. This loss can be reduced if the nuts are harvested before it reaches its complete maturity. In this context, present study was conducted in immature cashew kernel to find out suitable storage treatments to enhance the shelf life. Immature cashew kernels were stored in different concentrations of brine solution (5%, 10% and 15%), sugar syrup (50°B, 60°B and 70°B) and by drying in hot air oven until the moisture content of kernel reaches 2-3 per cent. Storage period was for four months and the observations like tannin content, microbial content and organoleptic qualities of kernels stored in each treatment were analysed at the beginning and at the end of the storage. The treatment with 10% brine and 70°B sugar syrup for four months were found as best for storing immature cashew kernels. Keywords: Cashew, immature kernels, organoleptic qualities and storage INTRODUCTION Cashew, an important horticultural crop of India, has a great socio-economic significance in this country. It is native of Brazil and the Lower Amazons. The demand for raw and processed cashew nut is high in both internal and export markets. According to the annual report of CEPCI (2019), India continued to be the largest producer of cashew nut in the year 2017- 2018 and the state that contributed maximum towards production is Maharashtra (33%), followed by Andhra Pradesh (14%), Kerala (11%) and Karnataka (11%). At present cashew kernels are consumed directly or used for various food preparations. Raw cashews contain 5% water, 30% carbohydrates, 44% fat and 18% protein. In a 100 gram reference quantity, raw cashews provide 553 Calories, 67% of the Daily Value (DV) in total fats, 36% DV of protein, 13% DV of dietary fiber and 11% DV of carbohydrates (USDA, 2015). The research efforts are concentrated mostly on mature kernels, and immature kernels gained little attention. In Kerala, sprouted cashew nuts are eaten as raw and also after cooking. Considerable quantities of cashew nuts are produced during rainy season in Kerala, especially in the late season flowering varieties like Madakkathara-2 and Sulabha, which are inferior in quality and are being wasted. The occurrence of late season flowering is mostly observed in hilly regions of Kerala like Wayanad and Idukki districts. The quality of nut is affected mainly by the infestation of pests and diseases during this season. It is estimated that more than 50% of the crop is lost annually due to pests and diseases in cashew (Haribabu et al., 1983). If the nuts are harvested before it reaches complete maturity and if those can be economically utilized, the loss during rainy season can be reduced to a great extent. Therefore, in this experiment, the immature nuts were harvested in tender form, when the shells were not hardened and were green in colour. The shell is soft and can be cut with a knife and kernel can be extracted. The kernels can be put to use in a variety of ways like serving as a snack or it could be relished as salads by combining with mango and sweets like tikka and cashew cake can be prepared 288 Jacob and Sobhana (Anandkumar et al., 2011). If we can store these immature kernels, it can be used round the year. In this context, the storage studies of immature cashew kernels were studied in College of Agriculture, Kerala Agricultural University, Thrissur, India. MATERIALS AND METHODS The immature cashew nuts were collected from Cashew Research Station, Madakkathara, Thrissur, India. Inflorescences of cashew tree were tagged at the time of anthesis and the nuts were harvested after 55 days. This is the stage before the nuts turn from green to grey colour. The harvested tender nuts were cut into halves using sharp knife and the kernels were scooped out. The outer covering of kernel (i.e.,) testa, was removed and these kernels were washed thoroughly in water followed by steam blanching for two to three minutes. Brine solutions of 5%, 10% and 15% concentrations were prepared and poured in sterilized glass bottles, to which the pre-treated kernels were added. Three replications were kept for each treatment. Similarly immature kernels were stored in sugar syrups of 50°B, 60°B and 70°B concentrations. Permissible qu a ntity of pr es er va tive wa s a dded to ever y treatments. Another treatment under storage studies was by drying. The pre-treated kernels were dried in hot air oven for two days, until the moisture content of the kernels were reduced to 2-3% and then stor ed in gla ss conta iners after complete cooling. Observations taken were tannin content, microbial content and organoleptic evaluation of kernels at first and fourth months of storage. Tannin content Tannin content of the kernels were estimated using Folin-Denis method at first and fourth months of storage. Microbial Count In microbial analysis, total bacterial, fungal and yeast counts were evaluated for both stored kernels and keeping solution. Organoleptic Evaluation Organoleptic evaluation was carried out by a panel of judges consisting of 15 members. The evaluation was carried out at beginning and ending of the storage per iod. Different orga noleptic pa r ameter s like appearance, colour, texture, flavour, taste, mouth feel and overall acceptability were evaluated. RESULT AND DISCUSSION Tannin content Tannin content could not be detected in any of the treated kernels at both first and fourth months of s tor a ge. It might be b eca us e of the pr ima r y processing steps like washing and steam blanching employed before storage treatments. Anand (1970) reported loss of tannins during pre-treatments like soaking, blanching and brining of fruits during p r epa r a t ion of a onla p r eser ve. Acc or ding t o Afoakwa et al. (2007), blanching of Bamba ra groundnuts before ca nning r educed the ta nnin content. Thus, these pre-treatments and storage tr ea tments wer e found to be ver y effective in r emoving ta nnin content of imma tur e ca shew kernels. Table 1. Microbial count of the keeping solution in different storage treatments Treatments Bacteria (107 cfu/ml) Fungi (103 cfu/ml) Yeast (104cfu/ml) 1 MAS 4 MAS 1 MAS 4 MAS 1 MAS 4 MAS 5% brine 7.67 (0.93) 6.33 (0.86) 1.67 (0.42) 4.67 (0.75) 1.33 (0.36) 1.67 (0.42) 10% brine 0.00 0.00 0.00 0.33 (0.10) 0.00 0.00 15% brine 0.00 0.00 0.00 0.00 0.00 0.00 50°B syrup 5.00 (0.77) 1.67 (0.26) 1.33 (0.36) 2.00 (0.48) 0.00 1.67 (0.42) 60°B syrup 0.00 0.00 0.00 0.00 0.00 0.00 70°B syrup 0.00 0.00 0.00 0.00 0.00 0.00 CD (5%) 0.11 0.32 0.20 0.14 0.07 0.10 MAS - Months after storage (Values in the parenthesis are logarithmically transformed) Cfu/ml - Colony forming unit per ml J. Hortl. Sci. Vol. 16(2) : 287-291, 2021 Treatments 289 Qualitative and organoleptic evaluation of immature cashew kernels under storage Microbial count The microbial count of both kernels and keeping solution is presented in table 1 and table 2. Among the seven treatments of storage, the bacterial count was beyond permissible limit both in the solution and kernels stored in 5% brine as well as in 50°B sugar syrup. The fungal population was within the acceptable limit for all the treatments. The yeast population was also found above the permissible limit for 5% brine and 50°B sugar syrup. This might be due to the less concentration of salt and sugar content in both these treatments which might not be sufficient to control the microbes. Ranken et al. (1997) reported that placing vegetables in 8-11% of salt content inhibited the microorganisms that may cause spoilage of vegetables. Thus, the immature cashew kernels can be stored for four months without microbial attack in 10% brine, 15% brine, 60°B sugar syrup and 70°B sugar syrup. Storage after drying was also found to contain permissible limit of microbial population. Organoleptic evaluation Among the seven treatments of storage, kernels stored in sugar syrup were the most accepted ones, both at the first and last months of storage (Fig 1). According to Ponting (1973), sugar uptake by the product kept in sugar solution, through osmotic process, modified the composition and taste of the fina l pr odu ct. In this ex per iment, the s cor es obtained for flavour (6.40-7.07) and taste (6.00- 7.07) were higher for kernels in sugar syrup. The uptake of sugar in the kernel might have resulted in the incr ea sed t a ste a nd fla vour lea ding to enhanced palatability and higher score. Kernels preserved in 70° B sugar syrup had the highest overa ll accepta bility scor e (7. 40) followed by kernels in 60° B (7.00) and 50° B (6.60) sugar syrup; higher sugar level might have resulted in more absorption. Kernel stored in 15% brine was the least accepted trea tment which might be due to its high salt content that made it unpalatable after four months of st or a ge. R os s e t a l. ( 2 00 2) r ep or ted t ha t macadamia kernel pieces, which were immersed in salt solution, became unacceptable on extended storage. Kernels in 10% brine was found better than 5% and 15% brine solutions in sensory parameters like appearance, colour, texture, flavour and overall acceptability. Ac c or ding t o H u t t on ( 2 0 0 2 ) , s a l t a c t a s a preserva tive against microbial growth and also impa r ts char a cter istic fla vour. All the qua lity parameters of organoleptic evaluation were found better for kernels stored in 10% brine after four months of storage compared to the first month. The preservative action of salt leading to enha nced storage life has been reported in many vegetables. Ba r wa l et a l. (2 00 5) r ep or ted tha t b la nc hed cauliflowers steeped in 10% and 15% salt solution were found acceptable up to 180 days. In dry storage, the dried kernels had an off taste after four months of storage which could be attributed to the rancidity of the kernels as experienced in nuts with Table 2. Microbial count of the kernels preserved in different storage treatments Treatments Bacteria (107 cfu/ml) Fungi (103 cfu/ml) Yeast (104cfu/ml) 1 MAS 4 MAS 1 MAS 4 MAS 1 MAS 4 MAS 5% brine 5.67 (0.82) 4.33 (0.73) 0.67 (0.20) 1.67 (0.42) 0.33 (0.10) 1.00 (0.30) 10% brine 0.00 0.00 0.00 0.33 (0.10) 0.00 0.00 15% brine 0.00 0.00 0.00 0.00 0.00 0.00 50°B syrup 3.33 (0.63) 1.00 (0.30) 3.33 (0.63) 3.00 (0.60) 0.33 (0.10) 1.67 (0.42) 60°B syrup 0.00 0.00 0.00 0.00 0.00 0.00 70°B syrup 0.00 0.00 0.00 0.00 0.00 0.00 drying 0.00 0.00 0.00 0.33 (0.10) 0.00 0.00 CD (5%) 0.04 0.03 0.12 0.17 NS 0.06 MAS - Months after storage (Values in the parenthesis are logarithmically transformed) Cfu/ml - Colony forming unit per ml NS - Non-significant J. Hortl. Sci. Vol. 16(2) : 287-291, 2021 Treatments 290 high fat content. According to Mexis and Kontominas (2009), the rancid taste of nuts during sensory evaluation occurred due to lipid oxidation. Young (2007) reported that rancidity was considered as the first sign of deterioration of nuts, since most edible nuts are rich in oil content. Hence the dried immature kernels cannot be used as such for consumption after a storage period of four months. CONCLUSION According to the findings, tender cashew nuts can be stor ed sa fely for f our months in pr eser ve (70°Brix) and also in 10% brine added with allowed preservatives. This can be followed anywhere in the world, where the mature nuts are affected due to any biotic or abiotic problems. ACKNOWLEDGEMENT The author s ar e ver y much grateful to Kera la Agr ic u lt u r a l Univer s it y, T hr is s u r, I ndia f or providing immense support and sufficient facilities for the conduct of this experiment. (a) (b) Figure 1. Effect of different treatments on sensory attributes immature cashew kernel at (a) first month and (b) fourth month of storage T 1 - Kernels preserved in 5% brine; T 2 - Kernels preserved in 10% brine; T 3 - Kernels preserved in 15% brine; T 4 - Kernels preserved in 50º brix sugar syrup; T 5 - Kernels preserved in 60º brix sugar syrup; T 6 - Kernels preserved in 70º brix sugar syrup; T 7 - Kernels preserved by drying REFERENCES Afoakwa, E. O., Budu, A. S. and Merson, A. B. 2007. Response surface methodology for studying the effect of processing conditions on some nutritional and textural properties of Bambara groundnuts (Voandzei subterranean) during canning. Int. J. Food Sci. Nutr. 58: 270-281. Anand, J. C. 1970. Retention of added vitamin C in amla preserves. Indian Food Packer 24(5): 19-20. Anandkumar, S., Kumar, V., and Anandraj, N. 2011. Processing and value addition of cashew nut [abstract]. 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Available: https://doi.org/10.1002/ 0471238961.1421201925152114.a01.pub2. [30th May 2020]. J. Hortl. Sci. Vol. 16(2) : 287-291, 2021 Qualitative and organoleptic evaluation of immature cashew kernels under storage 00 Contents.pdf 01 Shalini.pdf 02 Sheikh.pdf 03 Debanath.pdf 04 Nimbolkar.pdf 05 Satisha.pdf 06 Kaur.pdf 07 Nitin Kumar.pdf 08 Varsha.pdf 09 Ravishankar.pdf 10 Swamini.pdf 11 Vijaykumar.pdf 12 Usha bharathi.pdf 13 Yogalakshmi.pdf 14 Adams.pdf 15 Lakshman.pdf 16 Yella swami.pdf 17 Varalakshmi.pdf 18 Sharon.pdf 19 Lamesssa.pdf 20 Divya.pdf 21 Wani.pdf 22 Event Report.pdf 23 Index and Last Pages.pdf