Journal of large-scale research facilities, 2, A99 (2016) http://dx.doi.org/10.17815/jlsrf-2-100 Published: 02.12.2016 BioRef: The Re�ectometer for Biological Applications (V18) at BER II Helmholtz-Zentrum Berlin für Materialien und Energie * Instrument Scientist: - Dr. Marcus Trapp, Helmholtz-Zentrum Berlin für Materialien und Energie phone: +49 30 8062-43020, email: marcus.trapp@helmholtz-berlin.de Abstract: The time-of-�ight neutron re�ectometer BioRef is dedicated to the investigation of solid- liquid interfaces, in particular for soft matter applications. The possibility to mount a FTIR-ATR to the sample stage o�ers the possibility of combined in-situ measurements. 1 Introduction BioRef is a time-of-�ight neutron re�ectometer with strong focus on soft matter applications, in par- ticular at solid–liquid interfaces in the context of biological model systems under physiological condi- tions, including non-equilibrium situations. The instrument was built in joint e�ort of Ruprecht-Karls- Universität Heidelberg (RKU) and HZB within the “BMBF Verbundforschung” funding scheme. Unique features of BioRef are the chopper system, which allows for focusing on a selected Q-range in order to support fast kinetic studies, and the availability of simultaneous in-situ infra-red (IR) spectroscopy measurements. The latter complement the structural information provided by neutron re�ectivity (NR) with information on the molecules’ conformational order. The add-on IR spectroscopy unit allows for the in-situ investigations of Si-supported interfaces in ATR (attenuated total re�ection) geometry. A Bruker Vertex 70 infrared spectrometer is installed at the sample position for the very reason. The IR beam enters the Si substrate through the inclined top surface (45°) under 90° incidence, is then totally re�ected internally several times at the sample surface (front side) and the backside of the Si-substrate before leaving the substrate through its inclined bottom face and de�ected into an external IR-detector. The setup enables combined in-situ (kinetic) NR and IR studies with z-resolved depth pro�les from about 5 – 420 nm total thickness and conformational information on the embedded molecules acquired at the same time. *Cite article as: Helmholtz-Zentrum Berlin für Materialien und Energie. (2016). BioRef: The Re�ectometer for Biological Applications (V18) at BER II. Journal of large-scale research facilities, 2, A99. http://dx.doi.org/10.17815/jlsrf-2-100 1 http://jlsrf.org/ http://dx.doi.org/10.17815/jlsrf-2-100 http://dx.doi.org/10.17815/jlsrf-2-100 https://creativecommons.org/licenses/by/4.0/ Journal of large-scale research facilities, 2, A99 (2016) http://dx.doi.org/10.17815/jlsrf-2-100 Figure 1: View of V18. 2 Instrument application Typical applications are: • Solid-liquid interfaces • Combined NR and ATR-FTIR measurements • Time resolved NR 3 Instrument layout Figure 2: Schematic view of V18. 2 http://dx.doi.org/10.17815/jlsrf-2-100 https://creativecommons.org/licenses/by/4.0/ http://dx.doi.org/10.17815/jlsrf-2-100 Journal of large-scale research facilities, 2, A99 (2016) 4 Technical Data Neutron guide NL 3b Wavelength 90 Hz: 2.0 - 6.0 Å 45 Hz: 2.0 - 10.0 Å 25 Hz: 2.0 - 16.4 Å Wave length resolution Constant ∆λ /λ = 1 -5%, 7% - 11% Scattering plane Horizontal Range of re�ectivities 1x10−7 with a 50x80 mm2 sample Q resolution ∆Q/Q = 1.4 – 7% and 10 – 15% Detector 300 x 300 mm2 Multiwire PSD detector Polarized neutrons No Instrument options Possibility of combined NR and ATR-IR measurements Sample environment • Rectangular �ow cells (50x80 mm2) • Round �ow cells (∅ 60 mm) • Hydration chamber Table 1: Technical data of V18. 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BioRef: A versatile time-of-�ight re�ectometer for soft matter applications at Helmholtz–Zentrum Berlin. Review of Scienti�c Instruments, 82(5), 055101-9. http://dx.doi.org/10.1063/1.3581210 Trapp, M., Steitz, R., Kreuzer, M., Strobl, M., Rose, M., & Dahint, R. (2016). BioRef II—Neutron re�ectom- etry with relaxed resolution for fast, kinetic measurements at HZB. Review of Scienti�c Instruments, 87(10). http://dx.doi.org/10.1063/1.4964294 Wojciechowski, K., Orczyk, M., Gutberlet, T., Trapp, M., Marcinkowski, K., Kobiela, T., & Geue, T. (2014). Unusual penetration of phospholipid mono- and bilayers by Quillaja bark saponin 3 http://dx.doi.org/10.17815/jlsrf-2-100 http://dx.doi.org/10.1016/j.bbamem.2012.05.022 http://dx.doi.org/10.1021/acs.langmuir.5b01678 http://dx.doi.org/10.1088/1742-6596/251/1/012059 http://dx.doi.org/10.1088/1742-6596/251/1/012059 http://dx.doi.org/10.1063/1.3581210 http://dx.doi.org/10.1063/1.4964294 https://creativecommons.org/licenses/by/4.0/ Journal of large-scale research facilities, 2, A99 (2016) http://dx.doi.org/10.17815/jlsrf-2-100 biosurfactant. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1838(7), 1931 - 1940. http://dx.doi.org/10.1016/j.bbamem.2014.04.008 Wojciechowski, K., Orczyk, M., Marcinkowski, K., Kobiela, T., Trapp, M., Gutberlet, T., & Geue, T. (2014). E�ect of hydration of sugar groups on adsorption of Quillaja bark saponin at air/water and Si/water interfaces. Colloids and Surfaces B: Biointerfaces, 117, 60 - 67. http://dx.doi.org/10.1016/j.colsurfb.2014.02.010 4 http://dx.doi.org/10.17815/jlsrf-2-100 http://dx.doi.org/10.1016/j.bbamem.2014.04.008 http://dx.doi.org/10.1016/j.colsurfb.2014.02.010 https://creativecommons.org/licenses/by/4.0/ Introduction Instrument application Instrument layout Technical Data