7 476 siti chandra reka selly... Identification and Selection of Entomopathogenic Fungi as Biocontrol Agents for from South Sumatra Aphis gossypii SITI HERLINDA , CHANDRA IRSAN, REKA MAYASARI, SELLY SEPTARIANI* Aphid, is a vector of curly virus disease. The damage of chili due to its feeding is only 35% and it can achieved 100% if the damage caused by the aphid as a vector. The objectives of this research were to explore, to isolate, to identify, and to select entomopathogenic fungi as biocontrol agents for . The fungi were explored using insect bait in soil and collected infected insects from South Sumatra, Indonesia. Then, the fungi were isolated and identified, and finally the bioefficacy tests were done using 1 x 10 conidia mL against the third instar of The explorations found 25 isolates of enthomopathogenic fungi consisting 10 isolates of and 15 isolates of Selection of the fungi isolates on the aphid nymphs showed that isolate BPM isolated from caused the highest mortality rate (80.80%), while the lowest (47.20%) was caused by the isolate BAgTb isolated from The shortest time needed to produce 50% mortality (Lethal Time ) was 2.54 days (isolate of ). The longest time (3.66 days) was produced by isolate of . Key words: , , fungi AND Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Sriwijaya, Jalan Raya Palembang-Prabumulih, km 32, Ogan Ilir, Inderalaya 30662, South Sumatra, Indonesia Aphis gossypii A. gossypii A. gossypii. Beauveria bassiana Metarhizium anisopliae. Pseudoplusia chalcites A. gossypii. Chrysodeixis chalcites from Muarasiban Tenebrio molitor from Tanjung Raja Aphis gosypii Beauveria, Metarhizium 6 -1 50 T . he most important pest for chili is which is the vector of curly virus disease Aphid, is a vector of curly virus disease. The damage of chili due to its feeding is only 35% and it can achieved 100% if the damage caused by the aphid as a vector (Fuller . 1999). is a carrier for 76 viruses attacking various host plants (Satar . 1999) and has been reported resistant to various insecticides (Wang 2002). It is crucial to identify an alternative control that is relatively safer for both agricultural product and environ- mental health. Biological control is the main component of integrated pest management (IPM) that is a safer pest control than other control methods (Lopes . 2009). Biological control is needed for green consumers in the world who prefered pesticide free agricultural products. This can be achieved by controlled biologically of the vector insects using natural enemies, such as entomopathogen. and are common soil-borne entomopathogenic fungi that occur worldwide. cause a disease known as the white muscardine disease because infected insects covered with a layer of white mold (Alves 2002; Klinger 2006) and the green muscardine disease for (Santiago 2001). Both fungi attack the immature and adult stages of several insect orders, such as Hemiptera (Liu . 2002) and Diptera (Moraga . 2006). In order to develop successful biological control, a basic research is needed to find entomopathogenic fungi which are most pathogenic againts the vector, Therefore, the objectives of this research were to explore, to isolate, to identify, and to select entomopathogenic fungi as biocontrol agents for Aphis gossypii, Aphis gossypii et al A. gossypii et al et al. et al Beauveria bassiana Metarhizium anisopliae B. bassiana et al. et al. M. anisopliae et al. et al et al A. gossypii. A. gossypii MATERIALS AND METHODS Exploration of Entomopathogenic Fungi. Isolation and Identification of Entomopathogenic Fungi. Entomo- pathogenic fungi exploration was done by using two methods to obtain many species or strains of the fungi. The first method by collecting aphid nymphs and adults, larvae of Lepidoptera, Hemiptera, and other insect ordo that were sick or dead due to fungus infection (Herlinda 2008). The infected insects that showed symptoms of dry body and the presence of conidia and fungal conidia, white or green body of the larvae were isolated or purified. Surveys to explore the fungi were carried out five times on each location. Then, the fungus-infected insects were isolated in the laboratory at a cabinet of laminar air flow that had been sterilized with 70% alcohol. The second method of fungus exploration was to use insect as bait following method of Hashim and Azwana (2003). The insect used was third instar of (hongkong caterpillar) that had been newly molting. Soil used to trap the fungi was taken by purposive sampling from forests in South Sumatra. The soil sample was taken by digging at a depth of 50-10 cm, brought to the laboratory as much as 400 g, and then it was put into a plastic tray (13 x 13 x 10 cm ). The hongkong caterpillars were immersed 0.5 cm deep in the soil, and 20 larvae of the caterpillars were put in bottom of the tray. This treatment was repeated 20 times. Then, the tray was covered with a piece of black cloth that had been moistened. Three days later, the infected caterpillars were examined and isolated in the laboratory. Isolation of entomopathogenic fungi used methods of Herlinda (2006). The fungus-infected insects and caterpillars were sterilized with 1% sodium hypochlorite or 70% alcohol for three minutes. Then insects were rinsed with sterile water three times, and dried on top of sterile filter paper. Then, they were placed in a petri dish (diameter 9 cm) containing moist sterile paper and incubated to stimulate et al. Tenebrio molitor et al. 3 *Corresponding author, Phone +62-711-580663, Fax: +62-711-580276, Email: sherlinda_hpt_fp@unsri.ac.id ISSN 1978-3477 Vol 4, No 3, Dec 2010, p 137-142 I N D O N E S I A Available online at: http://www.permi.or.id/journal/index.php/mionline DOI: 10.5454/mi.4.3.7 conidial germination. Fungi were isolated, cultured on Saborroud Dextrose Agar (SDA) medium, and incubated for seven days at 25-27 C and relative humidity 80-85%. Then, a pure culture fungus was identified by using reference of Toledo (2010). The isolate was grown on slants of SDA medium supplemented with chitin from the small mole cricket, kept in 1.5 x 13 cm glass test tubes, and incubated for 7 days. The fungal spores were harvested from the slant culture, and each 1 g medium of the isolate was separately suspended in 9 mL water. Conidial density was calculated after analysis of a 1 mL sample of the suspension in a haemocytometer. Conidial germination as a measure of viability was obtained by spreading 1x10 propagules in 100 μL on glucose yeast agar (GYA) medium. The suspension was incubated at room temperature for 24 h. To calculate conidial viability per unit volume, total counts estimated with the haemacytometer were multiplied by the percentage of germination. After culturing isolates of the fungi, was also cultured on the chili to get the available colony that would be used to entomopathogenic fungi selection. The selection was done using method of Herlinda (2008). Conidia L fungal suspension (density of 1x10 conidia mL ) on the third instar of . In this experiment, ° et al. A. gossypii et al. A. Gossypii 6 6 -1 Selection of Entomopathogenic Fungi as Biocontrol Agents. of the fungal isolates were used by dripping topically 10 μ each isolate (Table 1) was inoculated on 25 newly molting- third instar of and repeated five times. Nymphs that had been exposed to the fungus conidia were subsequently maintained in plastic cylinders (diameter 9 cm and height 30 cm) covered with cloth. In the cylinders, there was a pot (diameter 9 cm) of chili growth. Every 6 h during the nymph stage, the number of dead nymph were recorded, while the number of nymphs growing to be adults were also recorded daily until all the nymphs became adult. The difference of mortality data and adult percent emerging were analyzed using analysis of variance, with the Tukey test. Time of nymph death was analyzed to determine the LT using probit analysis and also calculated by using SAS-STAT program Two species of entomopathogenic fungi from South Sumatra were identified, and consisted of 10 isolates, whereas were 15 isolates (Table 1) The fungi could be found in lowland and highland areas of South Sumatra, however they were more found in lowland areas than in highlands. Exploration method by dipping bait insecs in the soil was more effective than collecting infected insects from the field. isolates more often found during survey than isolates. A. gossypii B. bassiana M. anisopliae. B. bassiana M. anisopliae . M. anisopliae B. bassiana Data Analysis. Isolates of Entomopathogenic Fungi. 50 . RESULTS Table 1 Entomopathogenic fungi isolates collected from South Sumatra Isolate codes Source host insects Origins Exploration methods Lowland areas BAgTb Aphis gossypii Talang Buruk Collection of infected insects BNlPTr Nilaparvata lugens Pantura Collection of infected insects BTmSo Tenebrio molitor Soak Bait insects in soil BTmTb Tenebrio molitor Talang Buruk Bait insects in soil MaAgIn Aphis gossypii Inderalaya Collection of infected insects MaLaIn Leptocorisa acuta Inderalaya Collection of infected insects MAgIn Aphis gossypii Inderalaya Collection of infected insects MLaPTr Leptocorisa acuta Pantura Collection of infected insects MTmBb Tenebrio molitor Bukit besar Bait insects in soil MTmIn Tenebrio molitor Inderalaya Bait insects in soil MTmGb Tenebrio molitor Gelumbang Bait insects in soil MTmKt Tenebrio molitor Kenten Bait insects in soil MTmTk Tenebrio molitor Talang Kelapa Bait insects in soil MTmTr Tenebrio molitor Tanjung Raja Bait insects in soil Highland areas BLePd Lipaphis erysimi Pagardin Collection of infected insects BPM Chrysodeixis chalcites Muarasiban Collection of infected insects BPcPd1 Chrysodeixis chalcites Pagardin Collection of infected insects BPcPd2 Chrysodeixis chalcites Pagardin Collection of infected insects BTmPd Tenebrio molitor Pagardin Bait insects in soil BCcC Chrysodeixis chalcites Curup Collection of infected insects MAgPd Aphis gossypii Pagardin Collection of infected insects MTmBk Tenebrio molitor Bedeng Kresek Bait insects in soil MTmJr Tenebrio molitor Jarai Bait insects in soil MTmKj Tenebrio molitor Kerinjing Bait insects in soil MTmMs Tenebrio molitor Muarasiban Bait insects in soil Isolate code using first letter B refers to and M refers toBeauveria bassiana Metarhizium anisopliae. 138 HERLINDA ET AL. Microbiol Indones Isolate codes Conidial density (x 10 6 conidia mL -1 ) Range Mean±SE Beauveria bassiana BAgTb 36.60-37.40 37.00 ±0.40 ef BCcC 21.55-22.23 21.87 ±0.34 abc BLePd 43.30-45.15 43.98 ±1.02 f BNlPTr 20.60-43.35 21.53 ±0.54 abc BPM 37.15-59.60 45.39 ±12.36 f BPcPd1 24.60 -27.52 26.25 ±1.49 cd BPcPd2 20.60-22.83 21.61 ±1.13 abc BTmPd 42.17-43.65 42.73±0.80 f BTmSo 17.23-21.23 19.85±2.27 ab BTmTb 20.40 -24.52 22.81±2.15 abc Metarhizium anisopliae MaAgIn 19.08-20.25 19.63±0.59 a MAgIn 37.40-38.00 37.78±0.33 ef MAgPd 43.95-44.85 44.46±0.46 f MaLaIn 19.37-19.45 19.38±0.04 a MLaPtr 17.88-20.90 19.74±1.63 a MTmBb 18.75-20.45 19.36±0.95 a MTmBk 38.05-39.20 38.72±0.59 ef MTmGb 29.50-34.37 31.98±2.44 f MTmIn 35.70-38.47 38.96±3.53 ef MTmJr 36.87-38.70 37.62±0.96 ef MTmKj 42.45-44.70 44.06±1.40 f MTmKt 27.65-23.35 25.05±0.32 bc MTmMs 42.87-43.42 43.10±0.29 f MTmTk 18.00-18.63 18.36±2.29 a MTmTr 38.37-44.27 41.98±3.16 f Data in the same column followed by the same letter showed they were not significantly different (HSD test, P 0.05). and isolates had very dense conidia, but the trend of conidial density of isolates was significantly higher compared to isolates (Table 2). The highest conidial density of entomopathogenic fungi reached 45.39x10 conidia mL that was found on isolates coded BPcM, the lowest found on MTmTk isolate of (18.36x10 conidia mL ). The highest conidial viability of entomopathogenic fungi reached 47.50% with a 41.87% average viability that was found in isolates coded by BPcM (Table 3). The lowest conidial viability averaged of 11.90% found on isolates coded by MTmBb. The conidial viability of isolates tended significantly higher than those of isolates. Twenty five isolates were selected to determine the most virulent isolate nymphs. The results showed that all and isolates were pathogenic to the nymphs of causing mortalities between 42.40 and 80.80% (Table 4). The most virulent isolate was BPM of isolate causing an average of 80.80% mortality, and the two least ones were BAgTb of isolate and MLaPtr of isolate. mortality caused Conidial Density and Viability of Entomopathogenic Fungi. Virulence Isolates of Entomopathogenic Fungi. B. bassiana M. anisopliae B. bassiana M. anisopliae B. bassiana M. anisopliae B. bassiana M. anisopliae B. bassiana M. anisopliae againts A. gossypii B. bassiana M. anisopliae A. gossypii B. bassiana B. bassiana M. anisopliae A. gossypii 6 -1 6 -1 Isolate codes Percentage of conidial viability (%) Range Mean±SE Beauveria bassiana BAgTb 14.30-24.20 20.67±5.52 abcd BCcC 19.89-33.29 25.20±7.12 abcdef BLePd 20.00-28.10 25.20±4.51 abcdef BNlPTr 14.80-20.30 16.80±3.04 abc BPM 38.10-47.50 41.87±4.97 f BPcPd1 22.72-34.78 28.37±6.07 abcdef BPcPd2 14.83-31.37 21.50±8.72 abcde BTmPd 12.50-19.40 16.01±3.45 abc BTmSo 29.03-36.40 36.58±3.79 def BTmTb 26.30-40.90 31.5 ±8.16 bcdef Metarhizium anisopliae MaAgIn 5.49-21.37 13.15±7.95 ab MAgIn 10.50-15.15 13.15±2.39 ab MAgPd 15.40-23.50 18.83±4.19 abcd MaLaIn 9.69-18.28 14.80±4.52 abc MLaPtr 15.70-21.53 16.80±2.92 abcd MTmBb 8.71-14.47 11.9±2.93 a MTmBk 10.80-17.80 14.87±3.64 abc MTmGb 25.00-29.80 28.13±2.72 abcdef MTmIn 20.70-34.60 26.09±7.46 abcdef MTmJr 14.30-24.20 20.67±5.52 abcd MTmKj 20.00-27.02 23.61±3.51 abcdef MTmKt 28.00-43.50 32.21±7.89 cdef MTmMs 20.00-27.60 21.00±6.16 abcd MTmTk 15.68-26.71 21.70±5.58 abcde MTmTr 31.43-53.30 39.91±11.7 ef Table 3. Conidial viability of and M isolates Beauveria bassiana etarhizium anisopliae Data in the same column followed by the same letter showed they were not significantly different (HSD test, P 0.05). Volume 4, 2010 Microbiol Indones 139 Beauveria bassiana etarhizium anisopliae Table 2. Conidial density of and M isolates by BPM isolate was significantly different from BAgTb and MLaPtr isolates. BPcM isolate was isolated from in highland areas, BAgTb isolate from , and MLaPtr isolate from Both of the BAgTb MLaPtr isolates were from lowland areas. The level of mortality caused by treatment tended to be higher than those of treatment. All and isolates were able to infect nymphs and almost had low value of lethal time median (Lt ). The time median when death occured differed among isolates and it varied between 2.54 and 3.66 days. (Table 5). The result indicated that BPM isolate of had the lowest LT value (2.54 days) againts nymphs, while MTmTr isolate of was the highest one (3.66 days) In this study, exploration methods that were able to find entomopathogenic fungi were deeping insect bait in the soil and collecting the infected insects from the fields. Preliminary survey following method of Feng (2007) had tried to find the fungal conidia of entomopathogen from air using sticky cards but they were unable to be found. The fungi were easier to be found from the soil compared to infected insects from the fields. Herlinda . (2008) found that the entomopathogenic fungi obtained from infected Chrysodeixis chalcites Aphis gossypii Leptocorisa acuta. and A. gossypii B. bassiana M. anisopliae B. bassiana M. anisopliae A. gossypii B. bassiana A. gossypii M. anisopliae et al. et al 50 50 . DISCUSSION Isolate codes Nymph mortality (%) Range Mean ± SE Beauveria bassiana BAgTb 32-60 42.40±13.44 a BCcC 36-64 47.20±12.13 ab BLePd 24-72 52.80±17.29 ab BNIPTr 32-64 46.40±13.44 ab BPM 72-92 80.80± 7.69 b BPcPd 52-84 67.20±14.25 ab BPcPd2 32-64 47.20±13.38 ab BTmPd 36-52 44.80± 7.15 ab BTmSo 32-76 53.60±21.46 ab BTmTb 52-80 54.40±16.63 ab Metarhizium anisopliae MaAgIn 44-60 53.60± 6.69 ab MagIn 20-92 47.20±29.03 ab MagPd 32-68 48.00±16.73 ab MaLaIn 36-68 49.60±13.73 ab MLaPtr 32-64 42.40±17.57 a MTmBb 32-52 44.80± 7.69 ab MTmBk 52-60 53.60± 8.29 ab MTmGb 40-80 54.40 ±15.3 ab MtmIn 36-64 47.20±11.09 ab MTmJr 40-80 57.60±10.43 ab MTmKj 28-88 46.40±23.93 ab MTmKt 40-80 56.80±14.80 ab MTmMs 32-68 52.00±12.96 ab MTmTk 36-68 50.40±15.12 ab MTmTr 36-56 44.00± 7.48 ab Table 4 Mortality of nymph exposed to conidia of and at a concentration of 10 conidia mL Aphis gossypii Beauveria bassiana Metarhizium anisopliae 6 -1 Data in the same column followed by the same letter showed they were not significantly different (HSD test, P 0.05). 50Table 5 Lethal time median (LT ) of nymph exposed to conidia of and at a concentration of 1 x 10 conidia mL Aphis gossypii Beauveria bassiana Metharizium anisopliae 6 -1 Isolate codes Mean LT50 (days) 95% Confidence limit Regression Equation Lower Upper Beauveria bassiana BAgTb 3.18 2.97 3.50 y= 0.176+0.066x BCcC 3.02 2.73 3.53 y= 0.155 +0.061x BLePd 2.91 2.69 3.23 y= 3.070+1.060x BNlPTr 3.06 2.83 3.39 y= 0.167+ 0.065x BPM 2.54 2.42 2.67 y= 0.194+ 0.077x BPcPd1 2.80 2.67 2.96 y= 0.194 +0.075x BPcPD2 3.02 2.72 3.56 y= 0.194 +0.075x BTmPd 3.08 2.85 3.44 y= 2.980 +0.097x BTmSo 3.10 2.86 3.46 y= 0.185 +0.078x BTmTb 3.08 2.84 3.43 y= 0.157 +0.062x Metarhizium anisopliae MaAgIn 2.81 2.56 3.21 y= 0.142 + 0.058x MAgIn 3.03 2.78 3.42 y= 2.890 + 0.950x MAgPd 3.02 2.75 3.47 y= 0.188 + 0.079x MaLaIn 2.89 2.57 3.48 y= 0.144 + 0.058x MaLaPTr 3.11 2.80 3.71 y= 0.151 + 0.060x MTmBb 3.09 2.81 3.55 y= 0.155 + 0.062x MTmBk 2.95 2.75 3.25 y= 0.166 + 0.060x MTmGb 3.03 2.89 3.20 y= 3.060 + 1.010x MTmIn 3.14 3.10 3.89 y= 0.127 + 0.047x MTmJr 2.87 2.63 3.26 y= 3.050 + 1.060x MTmKj 3.20 2.93 3.39 y= 0.179 + 0.069x MTmKt 3.23 2.98 3.60 y= 0.135 + 0.049x MTmMs 3.07 2.92 3.27 y= 0.910 + 0.073x MTmTk 2.91 2.62 3.39 y= 2.820 + 0.970x MTmTr 3.66 3.36 4.13 y= 0.155 + 0.540x insects tended to be more difficult to be isolated. The other from the infected insects often were contaminated by air fungi. Hashim and Azwana (2003) reported that the conidia in the soil tended to be more persistent, they could be easily trapped using insect bait. Fuxa and Richter (2004) found that soils with high clay content improved persistence of the fungal conidia. Thus, future research using clay will be required to formulate the fungus conidia to increase efficacy as biological control. Condial density observed were densed, and the highest conidial density of entomopathogenic fungi reached 45.39x10 conidia mL that was found on isolates coded by BPcM. Soundarapandian and Chandra (2007) stated that conidial density were determined by mass production media and temperature of the incubated room. Liquid media tended to produce more conidia than those of solid ones. The optimum temperature and ideal pH for the mass production of was found to be 25-30°C and 7, respectively. Few germinated conidia were observed at 24 h of incubation that only reached 47.50%. The fungal isolates used in this study generally had low viability, it could be caused by shorter time (24 h) used in incubation of conidial suspension. Conidia were considered germinated if germtube lengths were two times in diameter of the propagules or if with conspicuous swelling (Toledo . 2010). They reported that germinated conidia in vitro for and at 72 hours could be 95.50% and 100%, respectively Bidochka . (2000) stated that 6 -1 B. bassiana M. anisopliae et al B. bassiana M. anisopliae . et al 140 HERLINDA ET AL. Microbiol Indones conidial viability was determined by temperature. The optimum temperature needed for entomopathogenic fungal conidia to germinate was 22-27 C with optimum humidity above 90%, and at under 86% humidity, the virulence would decrease continuously. Twenty five isolates of and found in this reasearch were almost pathogenic againts nymphs. The most virulent isolate was BPM of isolate causing an average of 80.80% mortality. BPcM isolate was isolated from that was unrelated to No relationship between pathogenicity and the origin of the isolates was observed. BagTb of isolate isolated from had the lowest pathogenicity and caused only 42.40% mortality of . Liu . (2002) also found that virulent isolates of or could be originally isolated from related and unrelated hosts. The ability of the BPcM isolate to produce the highest mortality rates might be caused either by their genetic characteristics, or by their conidial viability. Aregger (1992) stated that conidial viability factor might be the factor affecting virulence. Rate of loss of conidial viability of varies among the strain. Decline of conidial viability of this fungus correlated with decline of host mortality due to its infection. and isolates needed just 2.54 days and 2.81 days, respectively to kill . It takes shorter time than they killed other species of host, such as planthopper (Toledo 2010). Thompson and Brandenburg (2005) reported that death caused by the fungi usually occured more than 48 h after attachment of conidia to the insect cuticle. Toledo (2010) found that germ tubes on host cuticular surface began to be found at 24 and 48 h, and they were observed penetrating directly through the host cuticle in regions near the hairs of the second antennal segment and on the laterosternites of abdomen. After 72 h, long and errant germ tubes were detected on the cuticular surface. Fuxa and Richter (2004) stated that hyphae from conidia entered the host's body with the help of enzymes or mechanical pressure. In the end, the host was covered all over with propagules and the soft parts of the body were penetrated so hyphal growth could be observed outside the host insect's body. External hyphal growth would produce conidia which spread spores into the environment upon reaching maturity, then infect other healthy insects. We concluded that and were able to kill nymphs. Twenty five isolates of and found were almost pathogenic againts them. The most virulent isolate was BPM of isolate causing an average of 80.80% mortality. Dead insect hosts infected by showed the same symptoms as those infected by , except for the color of the hyphae which was greenish white We would like to thank Riyanto and Cheppy Wati for their assistance in the surveys. Financial support of this o B. bassiana M. anisopliae A. gossypii B. bassiana Chrysodeixis chalcites A. gossypii. B. bassiana A. gossypii A. gossypii et al B. bassiana M. anisopliae B. bassiana B. bassiana M. anisopliae A. gossypii Peregrinus maidis et al. et al. M. anisopliae B. bassiana M. anisopliae A. gossypii B. bassiana M. anisopliae B. bassiana M. anisopliae B. bassiana . 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