ISSN 1978-3477, eISSN 2087-8575 Volume 11, Number 1, March 2017 Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris Effect of Tempeh Supplementation on the Profiles of Human Intestinal Immune System and Gut Microbiota Studies for IAA (Indole-3-Acetic Acid) Production by Isolates H6 with Nitric Acid Mutation Analysis of Human Immune Response against Salivary Glands Protein Extract of Anopheles sundaicus. L in Malaria Endemic Area Construction and Expression of Single Recombinant Peptide Surfactant for Enhanced Oil Recovery (EOR) Application Martha Eka Cahyani, Is Helianti, Niknik Nurhayati, and Abinawanto Stephanie, Nine Kirana Ratih, Susan Soka, and Antonius Suwanto Rahayu Fitriani Wangsa Putrie, Tiwit Widowati, Sylvia JR Lekatompessy, and Harmastini Sukiman Mohammad Mirza Nuryady, Sugeng Setyo Utomo, Yunita Armiyanti, Sri Mumpuni Wahyu Widjajati, and Kartika Senjarini Cut Nanda Sari , Usman, Riesa KW Rohmat, Leni Herlina, Ken Sawitri Suliandari, Onie Kristiawan, Dwiyantari, Tati Kristianti, and Sony Suhandono 1 11 18 23 28 ISSN 1978-3477, eISSN 2087-8575 Volume 11, Number 1, March 2017 I N D O N E S I A Accredited at level “A” until Februari 2019 No. / /201040 P 4 Patron Siswa Setyahadi, 2020 Chief Editor Debbie S Retnoningrum, 2020 Editorial Board Members Antonius Suwanto, 2020 Brett Neilan, 2020 Dessy Natalia, 2020 Managing Editor Is Helianti, 2020 Astutiati Nurhasanah, 2020 Electronic Editor Iman Rusmana, 2020 Is Helianti, 2020 Business Manager Diana Nurani, 2020 Editorial Office Indonesian Society for Microbiology (Sekretariat PERMI) Room 124/TMC 2 DRN, Puspiptek-Serpong, Tangerang Selatan , Indonesia15314 Phone: +62-21-7560536 ext 7119 Fax: +62-21-7560694 E-mail: microbiology.indonesia@gmail.com URL: http://jurnal.permi.or.id/index.php/mionline Publisher Indonesian Society for Microbiology Published in March, June, September, and December. Subscription Prices for One Year, not including shipping and handling Indonesian Overseas Individual rate (IDR) 1 0 000,- 200 000,-5 Institutional rate (institution or library) (IDR) 240 000,- 400 000,- Bank Bank Mandiri Cabang Menara Thamrin, Jakarta, Acc PERMI; Acc No 103-0002080774 Printed by: CV. Istiqom Print Neung Tiaamroeng, 2020 Norio Kurosawa, 2020 Kartini Kramadibrata, 2020 Diana E Waturangi, 2020 Endang Purwantini, 2020 Wellyzar Sjamsuridzal, 2020 Yuan Kun Lee, 2020 Yaya Rukayadi, 2020 Netty Widyastuti Sigit, 2020 GENERAL EXECUTIVE BOARD OF INDONESIAN SOCIETY FOR MICROBIOLOGY 2015-2019 Advisory Board: Prof. Dr. Pratiwi Sudarmono, PhD, Sp.MK; Dr. Mohammad Dimyati; Prof. Dr. Endang Sukara; Prof. dr. Amin Soebandrio, PhD, Sp.MK; Prof. Dr. Ir. Betty Sri Laksmi Jenie, MS; Prof. Dr. Ir. Antonius Suwanto, MSc; Prof. Dr-Eng. Eniya Listiani Dewi, B. Eng, M.Eng; President: Dr. Siswa Setyahadi; Vice President: Prof. Fedik A Rantam, PhD; General Secretary: Diana Nurani, M.Si; Vice General Secretary: Drs. Nuki B Nugroho, M.Si; Treasurer: Dr. Niknik Nurhayati; Dr. Sylva Abraham; Scientific and Publication Committee: Dr. Debbie S. Retnoningrum; Dr. Is Helianti; Dr. Iman Rusmana; Prof. Dr. Marlina, Apt; Prof. Dra. Netty Widyastuti, M.Si; Dr. Ir. Nur Hidayat, MP; Drh. Mahdi Abrar; Dr. Supriyadi; Dr. Astutiati Nurhasanah; Tarwadi, MSc.; Certification Committee: Dr. Ir. Trismilah, M.Si; Dr. Erwahyuni Endah Prabandari; Ir. Dwi Kusuma Indriani, MP.; Dra. Dini Ryandini, M.Si; Dr. Ir. Maman Turjaman, DEA; Dr. Ernawati Giri Rahma; Dra. Harmastini Sukiman, M.Agr; dr. Purwati SpPD, Ph.D; Dr. Wahyu Purbowasito; Organization Advancement and Networking: Dr. Puspita Lisdiyanti; Prof. Dr. Ir. Endang S. Rahayu, MS; Sri Harjati Suhardi, PhD; Lia Yulia Budiarti, MKes; Dr. Chaidir; Dr. Retno Indrawati, drg., M.Si; Alit Pangestu, STP; Promotion and Advocacy Committee: Dra. MG. Isworo Rukmi, M.Kes; Jimmy Hariantono, PhD; Yawarsa; Gianina; Asri Sulfianti, MSi Cover Depan