12 (Yaya Rukayadi) Catatan penelitian (94-96).pmd SHORT COMMUNICATION The Effects of Xanthorrhizol on the Morphology of Candida Cells Examined by Scanning Electron Microscopy YAYA RUKAYADI∗ AND JAE-KWAN HWANG Department of Biotechnology, Yonsei University, 134-Sinchon-dong, Seodaemun-gu, Seoul 120-749, Korea The effects of xanthorrhizol, a natural anticandidal agent isolated from the rhizome of temulawak or java turmeric (Curcuma xanthorrhiza Roxb.) on the morphology of four human pathogenic Candida species, i.e., C. albicans, C. glabrata, C. guilliermondii, and C. parapsilosis was examined by scanning electron microscopy (SEM). The SEM analysis showed that, unlike control cells representing normal oval to spherical with smooth surface, treatment of Candida strains with xanthorrhizol at 1 x MICs (minimum inhibitory concentration) significantly affected the external morphology, exhibiting deformation, and protrusions on the cell surface. The potent anticandidal activity of xanthorrhizol may support the use of medicinal plants for the treatment of candidal infections. Key words: anticandidal, Candida sp., scanning electron microscopy, xanthorrhizol _____________________________________________ _________________ ∗ Corresponding author, Phone: +82-2-2123-4097, Fax: +82-2-362-7265, E-mail: yr32@yahoo.com The incidence of invasive fungal infections, particularly those caused by Candida sp., has increased over the past few decades (Hsueh et al. 2005). Recently, strains of Candida sp., such as C. albicans, C. glabrata, C. guilliermondii, and C. parapsilosis are showing increased resistance to traditional antifungal agents (Hawser and Dauglas 1995; Nguyen et al. 1996; Barchiesi et al. 1999; Dauglas 2003). This demonstrates the great importance of identifying novel antifungal agents (Ficker et al. 2003). Recent years have seen a growing interest in the use of natural antifungal agents isolated from the medicinal plants. Xanthorrhizol, a novel bioactive compound isolated from the rhizome of an indigenous Indonesian medicinal plant, temulawak or java turmeric (Curcuma xanthorrhiza Roxb.) has been previously reported to possess an antibacterial activity against several oral pathogens (Hwang et al. 2000a, 2000b) and has the ability to prevent and remove Streptococcus mutans biofilm formation (Rukayadi and Hwang 2006a, 2006b, 2006c). Xanthorrhizol also has anticandidal activity (Rukayadi et al. 2006), anti-Malassezia activity (Rukayadi and Hwang 2007a) and antimycotic activity against opportunistic filamentous fungi (Rukayadi and Hwang 2007b). This short communication reports the effect of xanthorrhizol on the morphology of Candida cells examined using the scanning electron microscopy (SEM). The Candida strains (C. albicans ATCC 10231, C. glabrata ATCC 50044, C. guillerimondii ATCC 9058, and C. parapsilosis ATCC 22019), used in this study, were obtained from the American Type Culture Collection (Rockville, MD, USA). The strains were cultured on Sabouraud dextrose broth (SDB) or Sabouraud dextrose agar (SDA) (Difco, Becton Dickinson and Company, USA) for 48 h at 35 oC. The standardized inoculum (a McFarland standard) for each isolate used was 5 x 106 cfu ml-1 (NCCLS 2002). Xanthorrhizol (Fig 1) (Hwang et al. 2000a) was isolated from the ethyl acetate fraction of the methanol extract from Curcuma xanthorrhiza according to the method of Hwang et al. (2000a). Briefly, the rhizomes of C. xanthorrhiza (100 g) were ground and extracted with 75% MeOH (v/v; 400 ml), and further fractioned consecutively with ethyl acetate (4.8 g), n-butanol (1.7 g), and water (1.1 g). Xanthorrhizol was isolated from the ethyl acetate fraction using silica-gel- column chromatography (Merk; 70-230 mesh; 5 x 43 cm; n- hexane/ethyl acetate, 10:1) yielding 0.2 g. Xanthorrhizol was dissolved in 10% (vol/vol) dimethylsulfoxide (DMSO) to obtain 1 mg ml-1 stock solutions. DMSO at 10% (vol/vol) was found not to kill Candida. Samples for scanning electron microscopy were provided as follows: 10 ml of cultures of C. albicans, C. glabrata, C. guilliermondii, and C. parapsilosis were exposed to 1 x MICs of xanthorrhizol (15, 10, 8, and 25 µg ml-1, respectively) (Rukayadi et al. 2006). After 1 hour of incubation at 35 oC, 1 ml of each culture, and a negative control of a corresponding culture were aliquoted and centrifuged at 3,900 x g for 10 min. Cell pellets were resuspended in 10 ml of sterile water and fixed overnight in 4% (vol/vol) glutaraldehyde. The samples were washed twice with 2-ml portions of sterile water and centrifuged at 3,900 x g for 10 1 4 4 OH 3 2 1 7 1 5 6 5 8 9 1 0 1 2 1 1 1 3 Fig 1 Structure of xanthorrhizol (Hwang et al. 2000a). MICROBIOLOGY INDONESIA, August 2007, p 98-100 Volume 1, Number 2 ISSN 1978-3477 min. The final pellets were then resuspended in sterile water. A drop of each suspension was transferred onto glass cover slips and fixed onto aluminium SEM stubs (Agar Science Ltd, Standstead, UK). The drop was spread thinly on the slip and dried in the air for 2 hour at room temperature. Graded concentrations of ethanol (70, 80, 90, and 95%, vol/vol), were applied, 2-5 min each, to ensure complete dehydration of specimens. The specimens were coated with gold in a low- pressure argon atmosphere employing a model E5000 Polaron Sputter Coating Unit (Polaron Equipment Ltd, New Haven, West Sussex, UK). A JEOL JSM-840 scanning electron microscope (Jeol Technics Ltd, Tokyo, Japan) was used to evaluate samples, operating at accelerating voltages of 20- 25 kV (Helal et al. 2006). SEM analysis showed that, unlike control cells (antifungal-agent free) showing normal oval to spherical shapes with smooth surfaces, treatment of Candida species with MIC of xanthorrhizol affected the external morphology of these yeasts (Fig 2). Control cells displayed well-formed cells with smooth unadulterated surface (Fig 2a, c, e, g). In contrast, cells incubated in the presence of xanthorrhizol demonstrated a greater tendency to clump compared with the control cultures (e.g., C. albicans - Fig 2b). Xanthorrhizol- treated C. glabrata cells showed minor abnormalities without a smooth or a slightly awkward surface (Fig 2d). Xanthorrhizol-treated Candida cells exhibited deformation and protrusions on the cell surface, which was more clearly demonstrated with C. guilliermondii and C. parapsilosis (Fig 2f, h). Electron micrographs revealed the existence of a recognizable affected external morphology of Candida cells caused by xanthorrhizol. Visible deformation, protrusion, or clumping was noted for each species at concentration MICs for 1 h treatment. In general, Candida exposed to xanthorrhizol at concentrations 1 x MICs exhibited substantial ultrastructural abnormalities such as shape deformation, protrusion, rugged cells surface, and clumping. Although, we were not able to identify the underlying molecular changes caused by the compounds by scanning electron microscope after 1 h treatment, we were able to show that the observable cell wall changes were generally obtained following exposure of the isolates to concentrations of xanthorrhizol equal to 1 x MICs. Analysis of electron micrograph at the appropriate exposure time and higher concentrations (2 x MICs or 4 x MICs) may result in more detailed analyses of the activities and effects of antifungal agents (Klepser et al. 1998). Further studies have been conducted examining the effect of xanthorrhizol on the morphology Candida cells at 2 x MICs and 4 x MICs for 2 and 4 h of incubation. In summary, the potent anticandidal action of xanthorrhizol against strains of four human pathogenic Candida species was demonstrated by scanning electron microscopy analysis. The results showed the usefulness of xanthorrhizol, a promising new antifungal agent for the topical treatment of candidiasis. Fig 2 SEM of Candida albicans (a and b), C. glabrata (c and d), C. guilliermondii (e and f), and C. parapsilosis (g and h) after treating by xanthorrhizol at 1 x MIC for 1 h of incubation. Volume 1, 2007 Microbiol Indones 99 a b c d e f g h REFERENCES Barchiesi F, Tortorano AM, Di Francesco LF, Cogliati M, Scalise G, Viviani MA. 1999. In vitro activity of five antifungal agents against uncommon clinical isolates of Candida spp. J Antimicrob Chemother 43:295-299. Douglas LJ. 2003. Candida biofilms and their role in infection. 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