item: #1 of 115
          id: njb-1
      author: Baral, Adesh; Gorkhali, Ritesh; Basnet, Amit; Koirala, Shubham; Bhattarai, Hitesh K
       title: Efficacy and Toxicity of Different Forms of Asparaginases Against Acute Lymphoblastic Leukemia: A Review
        date: 2021-12-30
       words: 6954
      flesch: 56
     summary: In a comparison done between intermittent and continuous routes of PEG E. coli asparaginase among 625 children of Europe, it was found that 95% of patients were asparagine depleted during treatment There were also specific toxicities seen in the group given PEG E. coli asparaginase.
    keywords: activity; acute; anti; arm; asparaginase; asparaginase activity; asparaginase group; average; baral; biotechnol; blood; bsn; cancer; cells; children; chrysanthemi; chrysanthemi asparaginase; clinical; coli; coli asparaginase; concentration; der; different; dose; e. coli; efficacy; enzyme; erwinia; escherichia; escherichia coli; et al; event; forms; free; grade; group; higher; hypersensitivity; induction; intramuscular; leukemia; lymphoblastic; native; native e.; native escherichia; nepal; njb; overall; pancreatitis; patients; peg; peg asparaginase; peg e.; peg escherichia; period; phase; range; reaction; recombinant; sluis; studies; study; survival; table; therapeutic; therapy; toxicity; treatment; trial; type; van; weeks; years
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        item: #2 of 115
          id: njb-106
      author:  Thapa, Aishwarya;  Budhathoki, Anupa;  Sapkota, Anupama; Sainju, Muskan; Shrestha, Prativa; Prasad Pant, Shyam
       title: Isolation, Identification and Screening of Bacillus species with Antimicrobial Activity from Different Soil Samples of Kathmandu Valley
        date: 2021-12-30
       words: 3695
      flesch: 50
     summary: Zone of inhibition demonstrated by antimicrobial metabolite producing isolates against test organisms Sample Isolate Number Name of the isolate S. aureus E. coli Pseudomonas spp. Hence, this study determines the soil in Kathmandu Valley as a potential source of antimicrobial producing Bacillus spp.
    keywords: activity; agar; antibiotic; antimicrobial; aureus; bacillus; bacillus spp; bacteria; biotechnol; bsn; characterization; coli; colonies; compounds; crude; different; esbl; extract; gram; growth; inhibition; inhibitory; isolates; isolation; kathmandu; method; motile; mrsa; negative; nepal; njb; nutrient; organisms; positive; primary; production; pseudomonas; resistance; salmonella; samples; screening; soil; species; spore; spp; study; test; thapa; valley; zone
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        item: #3 of 115
          id: njb-108
      author: Joshi, Bal Krishna; Ghimire, Krishna Hari; Bista, Prakash Raj; Yadaw, Ram Baran; Shrestha, Ram Krishna; Kharel, Gaurish Krishna; Paneru, Prakash; KC, Ram Bahadur; Bhandari, Deepak
       title: Intellectual Property Right on Basmati Rice: Current Scenario and Evidences of Origin, Diversity, Cultivation and Use Values of Basmati Rice in Nepal
        date: 2021-07-31
       words: 10753
      flesch: 63
     summary: Because of its high market value at global level, many countries and organizations have been attempting to get intellectual property rights (mainly patent and geographical indication tag) on Basmati rice. India applied for GI tag to Basmati rice in the European Union (EU) in July 2018, and Nepal submitted opposition letter along with proofs and evidences of origin, diversity, cultivation and use values of Basmati rice on 9 December 2020.
    keywords: 1981/08/31; 1994/09/22; 600; acharya; agricultural; agrobiodiversity; agronomy; ancient; areas; aromatic landraces; aromatic rice; ason; available; bajhang; bajura; bara; basmati; basmati dhan; basmati jhapa; basmati rice; basmati type; basmati::irgc; bhandari; bharatpur; bhatta; biodiversity; biotechnol; bird; bsn; center; chaudhary; chitwan; community; conservation; countries; crop; cultivation; dadeldhura; dec; dhan; dhan::irgc; different; districts; diversity; dna; doti; editors; et al; evidences; farm; farmers; figure; fine; gauchan; genebank; genetic; geographical; ghaiya; ghimire; gorkha; grain; group; hansaraj; high; historical; india; indication; information; international; internet; ipgri; irgc; jhapa; jhinuwa; joshi; joshi bk; joshi et; kailali; kalanamak; kalo; kanchanpur; kaski; kathmandu; khanal; khumaltar; krishna; lamjung; landraces; local; long; markers; market; masino; medium; morang; municipality; narc; national; native; nepal; nepal basmati; nepal j; nepalese; ngrc; njb; nuniya; origin; parsa; paudel; plant; pokhara; products; proofs; quality; ramechhap; rato; registered; research; resources; rice; rice grain; rice landraces; rice varieties; sali; science; seed; seto; shivasatasi; short; shrestha; singh; siraha; society; special; sthapit; subedi; sunaulo; sunsari; table; tag; tarai; technology; time; total; traditional; traits; tulsi; type; type rice; use; values; varieties; word
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        item: #4 of 115
          id: njb-109
      author: Kunwar, Saroj; Khatiwada, Saroj; Gelal, Basanta; Thapa, Saroj; Shah, Gaurishankar; Baral, Nirmal; Lamsal, Madhab
       title: Thyroid Function and Thyroglobulin Level in Iodine-Deficient Children of Eastern Nepal
        date: 2021-07-31
       words: 3060
      flesch: 61
     summary: Several studies, particularly in eastern Nepal show promising results in the improvement of iodine deficiency as revealed by an increase in median urinary iodine concentration (UIC) of school children In another study in eastern regions, Dhankuta and Dharan, iodine deficiency was 26.6% and 15.6% respectively among school children [13, 14].
    keywords: age; blood; children; concentration; data; deficiency; district; doi; eastern; free; ft3; ft4; function; gelal; health; hormones; hypothyroidism; insufficient; intake; iodine; lamsal; low; median; nepal; normal; nutrition; participants; range; reference; samples; school; serum; status; studies; study; subclinical; thyroglobulin; thyroid; tsh; uic; urinary
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        item: #5 of 115
          id: njb-112
      author: Pakuwal, Evance; Manandhar, Prakash
       title: Comparative Study of Nutritional Profile of Rice Varieties in Nepal
        date: 2021-07-31
       words: 5024
      flesch: 64
     summary: The results also highlight the cooking and physicochemical properties of rice depending on the amylose content of rice varieties. Among those, pigmented rice varieties such as red, black, purple, and brown rice are known to be consumed for a long time in Southeast Asian countries.
    keywords: activity; amylose; analysis; anthraquinone; antioxidant; available; bath; biotechnol; black; black rice; bsn; characteristics; content; cooking; determination; different; different rice; extract; food; glycoside; grains; highest; journal; jumli; length; manandhar; marsi; marsi rice; method; minutes; mixed; moisture; nepal; njb; nutritional; pakuwal; phenolic; photograph; phytochemical; pigmented; presence; present; properties; protein; red; respectively; results; rice; rice varieties; rice variety; sample; saponin; solution; standard; study; sugar; table; tannin; time; varieties; variety; water
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        item: #6 of 115
          id: njb-113
      author: Shrestha, Dhiraj; Bhattachan, Balkrishna; Parajuli, Hiramani; Shrestha, Sujata
       title: Avian/Bird flu: A review: H5N1 outbreaks in Nepal
        date: 2021-07-31
       words: 14271
      flesch: 57
     summary: Guan Y, Peiris JSM, Lipatov AS, Ellis TM, Dyrting KC, Krauss S, Zhang LJ, Webster RG, Shortridge KF: Emergence of multiple genotypes of H5N1 avian influenza viruses in Hong Kong SAR. Duan L, Bahl J, Smith GJD, Wang J, Vijaykrishna D, Zhang LJ, Zhang JX, Li KS, Fan XH, Cheung CL: The development and genetic diversity of H5N1 influenza virus in China, 1996–2006. Virology 2008, 380(2):243-254.
    keywords: acid; acute; agriculture; aiv; aivs; animal; antibodies; antibody; antigenic; antiviral; ards; areas; asia; avian influenza; biotechnol; birds; blood; bsn; care; case; cell; chan; change; chen; chickens; china; clade; clinical; close; collected; commercial; contact; contaminated; control; countries; current; days; dead; death; definition; detection; diagnosis; different; direct; disease; domestic; dose; drift; drug; ducks; e.g.; east; effective; engl; epidemiology; et al; evidence; evolution; exposure; feb; figure; genes; genetic; global; guan; guidelines; h1n1; h5n1; h5n1 avian; h5n1 infection; h5n1 influenza; h5n1 virus; h7n9; hand; health; health organization; hemagglutinin; high; hong; host; hpai; human; human h5n1; human infection; human transmission; illness; immune; infected; infection; influenza; influenza virus; international; isolation; j u; journal; jsm; kathmandu; kawaoka; kong; laboratory; lancet; layers; lee; like; lim; live; lower; major; management; mar; measures; meat; med; medical; microbiology; migratory; molecular; mortality; multiple; mutations; national; nature; nepal; nepal j; neuraminidase; new; njb; non; nphl; oie; onset; organization; oseltamivir; outbreak; pandemic; pandemic influenza; pathogenic; pathogenic avian; pathogenicity; patients; pcr; peiris; person; pneumonia; polymerase; population; possible; potential; poultry; preparedness; prevalence; preventive; probable; products; proper; protein; public; rapid; rate; recent; replication; research; respiratory; response; results; review; risk; role; samples; serum; severe; shrestha; sites; situation; smith; south; species; specific; specimen; spread; start; strains; studies; study; subtypes; surveillance; swab; symptoms; syndrome; table; test; therapy; threat; time; tract; transmission; treatment; update; vaccines; variation; veterinary; vietnam; viral; virology; virus; virus infection; viruses; vtm; water; webster; weeks; wild; workers; world; world health; years; zhang
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        item: #7 of 115
          id: njb-114
      author: Shrestha, Tara; Lamichhane, Janardan
       title: Assessment of phytochemicals, antimicrobial, antioxidant and cytotoxicity activity of methanolic extract of Tinospora cordifolia (Gurjo)
        date: 2021-07-31
       words: 3281
      flesch: 50
     summary: It is suggested that T. cordifolia methanol extract when taken orally has increased the erythrocytes membrane lipid peroxide and catalase activity [11] T. cordifolia is a well-known medicinal plant in traditional medicine, and several recent scientific investigations have highlighted its potential value in modern medicine. Brine shrimp lethality assay The toxic property of T. cordifolia methanolic extract was determined using the brine shrimp lethality
    keywords: 100mg; 25mg; 50mg; acid; activity; alkaloids; analysis; antibacterial; antimicrobial; antioxidant; ascorbic; bioassay; biotechnol; brine; bsn; concentration; cordifolia; cytotoxicity; disc; diseases; dmso; dpph; extract; extraction; figure; free; glycosides; inhibition; journal; jul; lamichhane; lc50; lethality; medicinal; methanol; nepal; njb; pharmaceutical; phytochemicals; plant; presence; radical; research; review; sample; scavenging; shrestha; shrimp; standard; stem; streptococcus; test; tinospora; traditional; triterpenes; zone
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        item: #8 of 115
          id: njb-115
      author: Maharjan, Bidhya; Karki, Shovana Thapa; Maharjan, Roshani
       title: Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children's Hospital
        date: 2021-07-31
       words: 7514
      flesch: 57
     summary: 57(98.28%) Doxycycline 0(0%) 2(3.44%) 56(96.56%) S. aureus isolates with complete resistance to Vancomycin (MIC≥16µg/ml) are termed as Vancomycin resistant S. aureus (VRSA).
    keywords: 2017; age; antibiotic; antimicrobial; association; aureus; azithromycin; bacteriological; biotechnol; bsn; care; cases; children; chloramphenicol; ciprofloxacin; clindamycin; clinical; coli; college; constitutive; correlation; culture; department; different; diffusion; disc; distribution; doi; doxycycline; drug; erythromycin; et al; figure; friendship; gram; group; growth; high; hospital; icu; increase; india; inducible; infection; international; isolates; journal; kathmandu; linezolid; maharjan; mcg; mdr; medical; meropenem; methicillin; microbiology; mrsa; mssa; multidrug; negative; nepal; njb; opd; organism; pant; patients; pattern; positive; prevalence; profile; pus; research; resistant; resistant staphylococcus; result; s. aureus; samples; sci; sensitive; shrestha; similar; spp; staphylococcus; staphylococcus aureus; strain; study; surgical; susceptibility; swab; table; tertiary; test; total; vancomycin; ward; wound; years
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        item: #9 of 115
          id: njb-116
      author: Adhikari, Bibek; Shah, Pradeep Kumar; Karki, Roman
       title: Antibiogram and Phytochemical Analysis of Cinnamon, Clove, and Sichuan Pepper Extracts
        date: 2021-07-31
       words: 5655
      flesch: 56
     summary: Several infectious diseases are treated using a wide range of plant extracts since they possess antimicrobial potentials. Alkaloids, flavonoids, phenolic compounds, and tannins are the essential phytochemicals present in the plants with possible therapeutic activities and these phytochemicals obtained in plant extracts have demonstrated antimicrobial potential against a wide range of infectious microorganisms
    keywords: absolute; acid; activities; activity; adhikari; aeruginosa; agar; albicans; analysis; antibacterial; antifungal; antimicrobial; antioxidant; aromaticum; aureus; biotechnol; bsn; candida; cinnamomum; cinnamon; clove; coli; concentration; content; different; distilled; effect; effective; essential; et al; ethanolic; eugenol; extracts; flavonoids; folin; food; gallic; gentamicin; higher; highest; india; inhibition; journal; mean; medicinal; methanol; method; microorganisms; mirabilis; nepal; njb; oil; pepper; pharm; phytochemicals; plant; polyphenol; potential; prepared; properties; property; proteus; pseudomonas; reagent; research; responsible; result; salmonella; sci; sharma; species; spices; standard; staphylococcus; study; syzygium; table; tannins; test; typhi; water; world; zanthoxylum; zoi; zone
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        item: #10 of 115
          id: njb-117
      author: K.C., Mamata; Lamichhane, Anuj
       title: Advances in Agricultural Biotechnology
        date: 2021-07-31
       words: 6648
      flesch: 50
     summary: Tissue culture has been used extensively to create genetic variability through the in-vitro culture of protoplasts, anthers, microspores, ovules, and embryos, to improve crop plants and to increase the number of desirable germplasm available to the plant breeder. Disease resistance genes are also transferred using somatic hybridization like asymmetric somatic hybridization was used to transfer bacterial blight resistance trait from wild Oryza meyeriana L. to Oryza sativa L. ssp.
    keywords: abiotic; acid; adoption; advances; agriculture; analysis; applications; available; bacterial; banana; biotechnology; blight; breeding; bsn; cells; certain; commercialized; concerns; conditions; content; cotton; countries; crops; cultivation; development; different; discovery; disease; dna; economic; effects; embryo; engineering; environmental; events; farmers; food; gene; genetic; genome; genomics; golden; haploid; health; herbicide; higher; human; hybridization; improvement; increase; insect; internet; jul; jun; lamichhane; line; maize; major; marker; material; molecular; nepal; new; njb; novel; number; omics; organisms; oryza; papaya; pest; plant; population; potato; potential; production; productivity; protein; quality; recent; rescue; resistance; review; revolution; rice; risks; sativa; sci; seeds; selection; somatic; soybean; species; specific; status; stress; structure; techniques; technologies; time; tissue; tolerance; tolerant; tomato; tools; traditional; traits; transfer; transgenesis; transgenic; transgenic crops; use; varieties; virus; wheat; wide; world; yield
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        item: #11 of 115
          id: njb-119
      author: Budhathoki, Shailesh; Shrestha, Anima
       title: Screening of Actinomycetes from Soil for Antibacterial Activity
        date: 2020-12-30
       words: 5935
      flesch: 48
     summary: Actinomycetes have broad spectrum antibacterial activity Among test organisms, Staphylococcus aureus was found to be the most susceptible with susceptibility toward 12 actinomycetes isolates followed by Klebsiella pneumoniae, susceptible towards 10 isolates of actinomycetes. Bar diagram showing susceptibility of test organisms towards actinomycetes isolates.
    keywords: -110; actinomycetes; actinomycetes isolates; active; activity; aeruginosa; agar; agents; agri; alkaline; antibacterial; antibiotic; antimicrobial; aureus; bacillus; biotechnol; brown; bsn; budhathoki; characterization; coli; colony; damp; days; dec; different; discovery; effective; findings; forest; fractions; gram; growth; india; inhibitory; isolates; isolation; journal; klebsiella; laboratory; land; metabolites; microbiology; moisture; mrsa; mssa; nepal; neutral; njb; novel; number; organisms; paratyphi; plates; pneumoniae; positive; potential; primary; production; range; research; resistant; salmonella; samples; screening; secondary; shrestha; soil; staphylococcus; staphylococcus aureus; strains; streptomyces; study; subtilis; susceptible; table; temperature; test; test organisms
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        item: #12 of 115
          id: njb-120
      author: Koirala, Barsha; Shrestha, Angela
       title: Comparative Study of Bioactive Compounds in Different Varieties of Pears in Nepal
        date: 2020-12-30
       words: 5011
      flesch: 62
     summary: Qualitative tests for sugars/carbohydrates in pear juice The various tests for sugars (Molisch’s test, Benedict’s test, Barfoed’s test, Seliwanoff test, Fehling’s test, Bial’s test, and Iodine test), protein test and phytochemicals (Catechins, Flavonoids, Cyclic glycosides, Terpenoids) were performed for pear juices. They were present in high amount in Chojuro, Kosui and Pharping pears.
    keywords: + +; absorbance; acid; acidity; analysis; anthocyanins; antioxidants; ash; barfoed; bartlette; benedict; bial; biotechnol; catechins; chinese; chojuro; clarity; color; concentration; content; cyclic; dec; denote; different; dye; flavonoids; food; fructose; fruits; glucose; glycosides; highest; iodine; juice; koirala; kosui; local; mixed; moisture; nepal; parameters; pears; pharping; phenolic; physicochemical; phytochemicals; positive; positive test; presence; red; research; sample; shrestha; solution; study; sucrose; sugars; table; tannins; terpenoids; test; total; value; varieties; vitamin; water; weight; yakumo
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        item: #13 of 115
          id: njb-129
      author: Pathak, Enish; Sanjyal, Arjun; Regmi, Chhatra Raj; Paudel, Saroj; Shrestha, Anima
       title: Screening of Potential Plant Growth Promoting Properties of Bacillus Species Isolated from Different Regions of Nepal
        date: 2021-07-31
       words: 3912
      flesch: 58
     summary: This study was aimed to isolate Bacillus species from soil of different regions of Nepal and screen for their ability to promote plant growth directly or indirectly by testing their ability to produce plant growth hormone indole acetic acid, hydrogen cyanide, ammonia and protease as well as phosphate solubilization. The isolated strains showed positive results to maximum PGPR traits and exhibited a potential to be used as alternatives to chemical fertilizers and pesticides and could be used as low-cost bio-based technology to promote plant growth in the agricultural sector.
    keywords: + +; ability; acid; agar; ammonia; bacillus; biotechnol; brown; chemical; colonies; color; cyanide; diameter; different; fertilizers; figure; filter; growth; halo; hcn; iaa; incubation; isolates; kathmandu; laboratory; light; medium; microbiol; nepal; njb; paper; pathak; pgpr; phosphate; pink; plant; plant growth; positive; production; properties; protease; reagent; rhizobacteria; samples; soil; solubilization; species; spp; strains; study; table; test; traits; width; yellow; zone
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        item: #14 of 115
          id: njb-132
      author: Gunasinghe, Y.H.K.I.S.; Rathnayake, I.V.N.; Deeyamulla, M.P.
       title: Plant and Plant Associated Microflora: Potential Bioremediation Option of Indoor Air Pollutants
        date: 2021-07-31
       words: 8828
      flesch: 46
     summary: Removal ratio of gaseous toluene and xylene transported from air to root zone via the stem by indoor plants. Can hydroculture be used to enhance the performance of indoor plants for the removal of air pollutants?
    keywords: activities; aerial; air; air pollutants; air pollution; air quality; area; aromatic; asparagus; associated; atmos; aureum; available; bacteria; benzene; biol; biological; biotechnol; bsn; building; cancer; capability; cause; chemical; cleavage; commutatum; compounds; concentration; conditions; contaminants; davallia; degradation; degrading; deremensis; dracaena; effects; efficiency; elsevier; endophytes; environ; environmental; epipremnum; et al; exposure; factors; fejeensis; ficus; filtration; fittonia; foliage; formaldehyde; formaldehyde removal; gaseous; growth; gunasinghe; health; hedera; helix; high; higher; hours; humans; hydrocarbons; h–1; illnesses; impact; increase; indoor; indoor air; indoor plants; initial; inorganic; internet; japonica; kays; kim; leaf; level; literature; liu; ltd; matter; mechanisms; methods; microbial; microdasys; microflora; microorganisms; mmol; m–3; m−2; nepal; nitrogen; njb; nose; nox; organic; ornamental; outdoor; particles; particulate; parts; philodendron; phyllosphere; phytoremediation; plant; plant species; podophyllum; pollutants; polluted; pollution; poor; potential; potted; ppm; problems; pseudomonas; purification; quality; range; reduction; ref; remediation; removal; res; research; review; role; root; safe; sansevieria; schefflera; sci; short; soil; sources; spathiphyllum; species; strategies; stress; studies; substances; systems; table; temperature; term; thiravetyan; throat; time; toluene; total; toxic; trifasciata; uptake; use; van; ventilation; voc; vocs; volatile; wallisii; xylene; zamiifolia; zamioculcas; zone
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        item: #15 of 115
          id: njb-133
      author: Shresta, Shrimita; Bhattarai, Bibek Raj; Adhikari, Bikash; Rayamajhee, Binod; Poudel, Pramod; Khanal, Santosh; Marasini, Bishnu P.; Aryal, Babita; Bhandari, Sudip; Parajuli, Niranjan
       title: Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants
        date: 2021-07-31
       words: 7666
      flesch: 49
     summary: Hence, the antibacterial activity of plant extracts reported here would be beneficial to identify some potent secondary metabolites as future drug candidates for the therapeutic measures of MDR-strains-induced infections in Nepal and beyond. To obtain plant extracts, the filtrates were concentrated in a rotary evaporator at 50 °C.
    keywords: 767; acacia; acid; activities; activity; adenophorum; aeruginosa; antibacterial; antibacterial activity; antimicrobial; antioxidant; ardens; atcc; aureus; australis; bioactive; biotechnol; biotechnology; bsn; catechu; chrysanthemum; clinical; coli; compounds; control; cordifolium; cumini; diabetes; dmso; drug; eclipta; effects; esculenta; eupoterium; evaluation; extracts; food; free; gae; guajava; highest; hypericum; ic50; indica; indicium; journal; kathmandu; leaves; lee; m. s.; mangifera; marsupium; mdr; medicinal; medicinal plants; microbiology; morus; myrica; neomycin; nepal; njb; officinale; pathogens; phytochemical; plant; plant extracts; pneumoniae; properties; prostrata; psidium; pterocarpus; quercetin; resistant; robusta; scavenging; shorea; shrestha; smallanthus; sonchifolius; species; standard; strains; study; syzygium; table; tfc; tpc; treatment; urtica; value; xanthomonas; zingiber; zoi
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        item: #16 of 115
          id: njb-16
      author: Poudel, Santosh; Paudyal, Akash; Sharma, Bishnu Prasad; Sharma, Kamana; Baral, Yubaraj; Adhikari, Shailaja; Shakya Hada, Manju Shree 
       title: Microbial and Physico-Chemical Quality Assessment of Rivers of Kathmandu Valley
        date: 2021-12-30
       words: 5118
      flesch: 63
     summary: The main objective of the study is to detect the physio-chemical and microbiological parameters of water sample from the Bagmati river and its tributaries of Kathmandu valley along with antibiotic susceptibility. Physical Parameters of water samples.
    keywords: alkalinity; ammonia; analysis; antibiotics; assessment; available; bagmati; bagmati river; balkhu; biotechnol; bishnumati; bod; bsn; chemical; chloride; coli; coliform; dec; dhobi; dissolved; guidelines; hanumante; higher; highest; human; i.e.; isolates; journal; kathmandu; level; lowest; method; microbiological; nepal; njb; oxygen; parameters; phosphate; physico; pollution; poudel; previous; quality; r r; r s; resistance; river; s s; sample; study; sundarijal; susceptibility; t u; temperature; total; treatment; tributaries; tukucha; turbidity; u r; valley; value; wastewater; water
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        item: #17 of 115
          id: njb-161
      author: Amgai, Resham B.; Pokharel, Shreejan; Pantha, Sumitra; Parajuli, Atit; Subedi, Sudeep; Dhital, Shambhu P.
       title: Selection of Pyramided Barley Advanced Lines for Stripe Rust, Leaf Rust and Crown Rust Diseases Using Molecular Markers
        date: 2020-12-30
       words: 2892
      flesch: 63
     summary: Therefore, one polymorphic sequence tagged sites (STS) marker (ABG054) for stripe rust resistance, two polymorphic simple sequence repeats (SSR) markers (Bmac0144h and HVM049) for leaf rust and one polymorphic SSR marker (Bmag0006) for crown rust resistance were used to select the advanced barley lines (at F8 stage) from above parents. Selection, identification and incorporation of rust resistance genes is the only option for the development of rust resistance barley varieties for Nepal.
    keywords: 115; abg054; acc#2478; advanced; barley; biotechnol; bold; bonus; breeding; bsn; crosses; crown; dec; disease; field; figure; gene; hordei; hvm049; leaf; lines; major; marker; mas; microsatellite; min; molecular; nepal; nepalese; njb; note; parents; pcr; polymorphism; pyramided; pyramiding; research; resistance; rust; selection; solu; ssr; stage; stripe; table; uwa; variety
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        item: #18 of 115
          id: njb-170
      author: Pawar, Rasika; Zambare, Vasudeo; Nabar, Bela
       title: Comparative Assessment of Antibiotic Resistance in Lactic Acid Bacteria Isolated from Healthy Human Adult and Infant Feces
        date: 2020-10-22
       words: 4458
      flesch: 48
     summary: Antibiotic resistance was exhibited by lactic acid bacteria against most commonly used antibiotics and it was higher in strains isolated from adult fecal samples than in the strains isolated from infant fecal samples. The increasing trend in antibiotic resistance from infant to adult might be due to food habits and antibiotic intakes.
    keywords: acid; acid bacteria; adult; agar; ampicillin; analysis; animal; antibiotic; antibiotic resistance; antimicrobial; assessment; bacteria; biotechnol; bsn; cefotaxime; cefuroxime; chloramphenicol; ciprofloxacin; clinical; colonies; colonization; cultures; data; dec; development; enterococcus; erythromycin; feb; fecal; feces; food; gentamycin; gut; health; higher; human; india; infant; infant fecal; isolated; issue; journal; lab; lactic; lactic acid; lactobacillus; levels; microbiology; microflora; nepal; njb; pawar; pediococcus; penicillin; plates; present; probiotics; resistance; safety; samples; sensitive; software; special; spp; strains; study; susceptibility; tobramycin; vancomycin; whonet; world
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        item: #19 of 115
          id: njb-171
      author: Bhalshakar, Chhaya
       title: Study of the Impact of Organic Manures and Biofertilizers on growth of Phaseolus aureus Roxb.
        date: 2020-10-22
       words: 4011
      flesch: 66
     summary: The biological manure helps to increase crop yields, and also plays a vital role in the nutrient accessibility in soil by improving the physical, chemical, and biological structure of soil, and it enhances the utilization of applied fertilizers Weed plants compete with the agricultural crops; they cause a tremendous reduction in crop yields and increase their production costs.
    keywords: achyranthes; agriculture; analyses; atcb; atvb; aureus; azotobacter; bacteria; bhalshankar; biod; biofertilizer; cake; chemical; con; control; crop; dose; dry; efficiency; fertilizers; fresh; growth; ha-1; higher; highest; increase; india; journal; kg ha-1; lowest; manures; mixed; ncb; neem; neemcake; nepal; nitrogen; npk; organic; phosphate; plant; production; ratio; roxb; significant; single; soil; table; tephrosia; total; treatment; vermicompost; weed; weight; yield
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        item: #20 of 115
          id: njb-174
      author: Suwal, Meena Maiya; Lamichhane, Janardan; Gauchan, Dhurva Prasad
       title: Regeneration Technique of Bamboo Species through Nodal Segments: A Review
        date: 2020-07-31
       words: 11693
      flesch: 65
     summary: The effect of coconut water on bamboo shoot multiplication has been reported by different researchers. Future research must be focused to generate a large scale of bamboo plants.
    keywords: 3mg; acid; ads; arya; ascorbic; asper; axillary; b. balcooa; balcooa; bamboo; bamboo species; bambusa; bap; better; biology; biotechnology; break; browning; bsn; bud; cell; coconut; collection; combination; concentration; condition; contamination; coumarin; culm; culture; dendrocalamus; development; different; edible; effective; embryogenesis; establishment; et al; explants; factors; field; forest; giganteus; growth; hamiltonii; hardening; high; hormones; iaa; iba; important; induction; influence; initiation; jan; journal; jul; kalia; l naa; l y; large; liquid; liquid ms+; mass; mass multiplication; mature; media; medium; method; mg/ l; micropropagation; mother; ms media; ms+ bap; ms+ iba; ms+ naa; multiplication; munro; naa; nees; negi; nepal; njb; nodal; nodal explants; nutans; organ; period; plantlets; position; problem; production; proliferation; protocols; ramanayake; rapid; rate; regeneration; regulators; researchers; review; rooting; roxb; saxena; scale; science; season; segments; sharma; shoot; shoot multiplication; singh; size; soil; solid; somatic; sood; species; strength; strictus; sucrose; supplement; surface; suwal; tdz; technique; tissue; treatment; tulda; u l; vitro; vulgaris; wall; water; world; years; ½ ms+
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        item: #21 of 115
          id: njb-175
      author: Kumari, Rinki; Dubey, G.P.
       title: Standardization of Ayurvedic Drug- Nyctanthes arbor-tristis, Hippophae salicifolia, Ocimum tenuiflorum and Reinwardtia indica and Combined Herb-Herb
        date: 2020-07-31
       words: 10500
      flesch: 62
     summary: In India, Ayurvedic Pharmacopeia is not progressively sufficient to guarantee the quality and virtue of plant extracts’ use as a herbal drug. R. indica extract is widely used by local communities for different purposes such as for tongue wash, to bring about an increase in - lactation period, a remedy against skin diseases, as an anti-infection agent etc.
    keywords: + +; absent; academy; acetic; acharya; acid; agar; agnivesha; alcohol; analysis; arbor; area; ash; ayurvedic; biotechnol; black; black powder; blue; brown; brown brown; brown green; brown powder; bsn; carbohydrate; cells; charaka; chowkhamba; clinical; collected; color; combined; compounds; content; dark; dark brown; different; dridhabala; drug; dubey; editors; epidermis; estimation; ethanol; ether; evaluation; extract; extractive; figure; flavonoids; fluorescence; formulation; fruit; green; green black; green green; green powder; greenish; hcl; health; herb; herbal; hippophae; hptlc; hrs; hydroalcoholic; indica; inorganic; insoluble; j u; journal; krishnadas; kumari; leaf; light; light brown; light yellow; like; material; matter; medicinal; medicine; method; n. arbor; naoh; nepal; nepal j; njb; nmt; parameters; parenchyma; peak; percentage; petiole; phytochemical; plant; polyherbal; powder; presence; present; proximate; quality; r. indica; results; salicifolia; samhita; sample; section; single; soluble; solution; standard; standardization; sthana; study; sulphated; sulphuric; sutra; system; table; tenuiflorum; test; total; track; traditional; transverse; treatment; trichomes; tristis; value; vascular; vyt; water; wavelength; weight; yellow; yellow powder; yellowish; yellowish brown
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        item: #22 of 115
          id: njb-176
      author: Mohammed, Messaoudi; Maroua, Merah
       title: Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits
        date: 2020-07-31
       words: 4546
      flesch: 59
     summary: Tests of antioxidant capacity The antioxidant capacity of the four varieties were evaluated using free radical scavenging test (DPPH) and total antioxidant capacity (TAC). Total antioxidant capacity of fig flowers fruits methanolic extracts.
    keywords: absorbance; acid; activity; analysis; anthocyanins; antioxidant; antioxidant capacity; ascorbic; bakor; bechar; biotechnol; bsn; capacity; carica; chemistry; compounds; content; correlation; ctl; des; determination; differences; different; dpph; dry; extracts; ficus; fig; figs; figure; flavonoids; flowers; food; free; fruits; gae; high; human; ic50; inhibition; journal; level; mascara; medicinal; meoh; meoh var.2; merah; messaoudi; methanolic; min; nepal; njb; phenolic; phytochemicals; plant; positive; radical; samples; scavenging; significant; solution; species; tac; tannins; test; tfl; total; total antioxidant; tpl; value; var.2; varieties; variety; weight
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        item: #23 of 115
          id: njb-178
      author: Hori, Kengo; Devkota, Hari Prasad
       title: Phenolic Compounds from the Aerial Parts of Adenophora triphylla (Thunb.) A. DC. var. triphylla and their Free Radical Scavenging Activity
        date: 2020-07-31
       words: 2692
      flesch: 63
     summary: japonica (Regel) H. Hara (“Tsuriganeninjin” in Japanese) are among many varieties of plant A. triphylla (Thunb.) Free radical scavenging activity of the extract and compounds was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity method.
    keywords: acid; activities; activity; adenophora; aerial; chemical; compounds; devkota; dpph; extract; flavonoids; foods; free; functional; gel; isolated; japan; japonica; kumamoto; leaves; lh-20; luteolin; meoh; nepal; nmr; parts; phenolic; plant; potential; radical; research; scavenging; sephadex; study; table; thunb; triphylla; triphylla var; var
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        item: #24 of 115
          id: njb-180
      author: Rai, Shyamala; Karki, Barsha; Humagain, Sujita; Rimal, Sandesh; Adhikari, Sandhya; Adhikari, Shilpa; Thapa, Suchitra
       title: Antibiogram of Escherichia coli and Staphylococcus aureus Isolated from Milk Sold in Kathmandu District
        date: 2020-12-30
       words: 3861
      flesch: 52
     summary: [7, 11] in milk samples but in the case of Nepal very less studies are found on antibiotic resistance of milk isolates. Total bacterial count of milk samples The collected milk samples were serially diluted up to- 10-8 dilution.
    keywords: acid; ampicillin; analysis; antibiogram; antibiotic; antimicrobial; aureus; cefoxitin; ceftriaxone; cfu; chloramphenicol; ciprofloxacin; coli; dec; distribution; district; e. coli; esbl; escherichia; figure; food; health; higher; infections; isolates; journal; kathmandu; load; mdr; medium; microbial; microbiology; milk; milk samples; mrsa; nalidixic; nepal; pasteurized; pathogenic; presence; prevalence; quality; raw; raw milk; resistance; s. aureus; samples; standard; staphylococcus; studies; study; susceptibility; tbc; tetracycline; total; yes
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        item: #25 of 115
          id: njb-182
      author: Devkota, Surya Prasad; Paudel, Ashmita; Gurung, Krishna
       title: Vancomycin Intermediate MRSA Isolates Obtained from Retail Chicken Meat and Eggs Collected at Pokhara, Nepal
        date: 2019-12-29
       words: 3803
      flesch: 49
     summary: S aureus was isolated from eggshells and chicken meat samples collected from different retail outlets of the Pokhara metropolitan. Isolated yellow colonies were further examined by Gram-staining, catalase, and coagulase test to detect S aureus.
    keywords: agar; animal; antibiotic; antimicrobial; aureus; biotechnology; chicken; china; clsi; concentration; detection; devkota; egg; eggs; erythromycin; food; health; humans; inoculated; intermediate; isolates; items; journal; meat; methicillin; mic; mrsa; mrsa isolates; mssa; nepal; nepjol.info/index.php/njb; njb; pokhara; prevalence; range; resistance; retail; s aureus; samples; sensitive; society; staphylococcus; staphylococcus aureus; sterile; study; susceptibility; table; test; use; value; vancomycin; vol
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        item: #26 of 115
          id: njb-189
      author: Chhetri, Til Kumari; Subedee, Bijay Raj; Pant, Bijaya
       title: Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability
        date: 2019-12-29
       words: 7065
      flesch: 62
     summary: Increasing the efficiency of the use of available soil nitrogen can meet the additional Nitrogen demand by making cereal plants capable of fixing its own nitrogen through close Nepal Journal of Biotechnology. Apart from direct benefit from effective nitrogen fixation [4] legumes and Rhizobium provides added value in weed, pathogen and insect control when rotated with crop in farming system [5] together with improving soil structure and increasing soil organic matter content
    keywords: agar; agricultural; air; alginate; average; bacteria; beads; beaker; biochemical; biological; biotechnology; bradyrhizobium; broth; cells; chhetri; colonies; colony; color; concentration; congo; crop; culture; days; different; dilution; disc; effective; encapsulation; environmental; fixation; fixing; form; growth; hours; immobilized; inoculants; inoculated; inoculation; inoculums; japonicum; journal; legumes; mass; media; minutes; months; nacl; nepal; nepjol.info/index.php/njb; nitrogen; nitrogen fixation; njb; nodules; number; organism; peat; plant; plates; prepared; present; production; pure; research; result; rhizobia; rhizobium; root; rotation; seed; slow; society; sodium; soil; solution; species; sterile; strain; study; sucrose; surface; survival; test; tolerance; use; viability; viable; vol; water; yema
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        item: #27 of 115
          id: njb-190
      author: Bhandari, Sudip; Rayamajhee, Binod; Dhungel, Laxmi; Poudel, Sami; Gaire, Bhagwati; Shrestha, Sunil; Parajuli, Niranjan
       title: Infectious sources of Histoplasmosis and molecular techniques for its identification
        date: 2019-12-29
       words: 4230
      flesch: 56
     summary: Notably, in tuberculosis- endemic regions, disseminated histoplasmosis can easily be misdiagnosed as tuberculosis because of its similar clinical symptoms and must be considered as histoplasmosis infection in patients who do not respond to empiric anti- tubercular therapy [20]. The real-time RT-PCR or qPCR shows promising results in the diagnosis of histoplasmosis infection, which currently identified the H. capsulatum among several other fungi
    keywords: aids; assay; bhandari; biotechnology; body; capsulatum; cases; clinical; country; daily; detection; diagnosis; disease; dissemination; dna; endemic; form; fungal; fungus; health; high; histoplasma; histoplasmosis; hiv; identification; immune; india; infection; journal; kauffman; lack; med; microbiol; molecular; mycelial; national; nepal; nepjol.info/index.php/njb; njb; number; organ; patients; pcr; people; population; probe; progressive; proper; public; pulmonary; reaction; real; research; review; risk; samples; sector; severe; significant; society; soil; spores; strategies; symptoms; technique; time; transplant; treatment; update; vol; wheat; years; yeast
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        item: #28 of 115
          id: njb-195
      author: Subedi, Deepak; Devkota, Surya Prasad; Bhatta, Dharm Raj; Sharma, Binita Koirala; Paudel, Ashmita; Gurung, Krishna; Gautam, Damodar
       title: Antibiotic Susceptibility Profile of respiratory pathogens obtained at tertiary care hospital from western Nepal
        date: 2019-12-29
       words: 3314
      flesch: 47
     summary: Keywords: respiratory pathogens, western Nepal, Pseudomonas aeruginosa, Acinetobacter spp, Klebsiella pneumonia, S. aureus. Surveillance study on respiratory pathogens was imminent in this part of the nation.
    keywords: acinetobacter; aeruginosa; antibiotic; antimicrobial; aureus; biotechnology; candida; care; clindamycin; colistin; drug; erythromycin; gram; hospital; infections; int; isolates; journal; klebsiella; lower; med; microbiol; negative; nepal; nepjol.info/index.php/njb; njb; pathogens; patients; pneumoniae; pokhara; positive; profile; pseudomonas; pyogenes; resistance; respiratory; samples; sensitive; society; spp; sputum; staphylococcus; streptococcus; study; subedi; susceptibility; table; tertiary; test; throat; tract; vancomycin; vol
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        item: #29 of 115
          id: njb-2
      author: Shrestha, Bijay Kumar; Shakya, Jenish
       title: Simple method devised for rapid isolation and identification of Vibrio cholerae from water resources of Sunsari District, Nepal
        date: 2021-12-30
       words: 4313
      flesch: 57
     summary: This study aimed to study the incidence of Vibrio species and employ simple method for rapid detection of Vibrio cholerae from water samples of Sunsari, Nepal. Therefore, the aim of this study was to study the incidence of Vibrio species and devise simple method for rapid detection of Vibrio cholera from water samples of Sunsari, Nepal.
    keywords: agar; alginolyticus; alkaline; arginine; biochemical; biotechnol; bsn; chloride; cholerae; citrate; collected; colonies; colony; contamination; culture; devoid; different; diseases; distribution; district; doi; drinking; environment; fermenting; fluvialis; health; hours; identification; incidence; isolated; isolation; journal; media; method; microbiology; nacl; nepal; njb; non; nutrient; outbreak; oxidase; pond; presumptive; public; rapid; resource; samples; sewage; shakya; shrestha; simple; sodium; species; strains; study; sucrose; sunsari; table; tap; tcbs; test; tube; vibrio; vibrio cholerae; water; water samples; yellow
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        item: #30 of 115
          id: njb-203
      author: Suwal, Shyam; Marciniak, Alice
       title: Technologies for the Extraction, Separation and Purification of polyphenols – A Review
        date: 2019-01-15
       words: 9372
      flesch: 51
     summary: ©NJB, Biotechnology Society of Nepal 81 Nepjol.info/index.php/njb on polyphenols extraction but considerable innovations are needed for their industrial and economic feasibility. The irradiation of plant tissues with ultrasound increase the number of extraction solvent exposed to the molecules by breaking down the cell wall, increasing swelling and hydration degree thus improving the diffusion and mass transfer [19].
    keywords: 2010; acids; activity; addition; anthocyanins; antioxidant; application; assisted; autheor; bazinet; bioactive; biotechnology; cell; chem; chemical; chromatogr; citrus; comparison; compounds; concentration; content; conventional; cost; cranberry; degradation; different; diseases; distillation; e c; effect; efficiency; electric; electrodialysis; eng; et al; ethanol; exchange; extraction; extraction process; field; figure; filtration; flavonoids; fluid; food; functional; grape; green; health; heat; high; higher; hydrostatic; iec; increase; industrial; interest; journal; juice; known; leaves; liquid; low; mae; material; membrane; methods; microwave; min; molecular; molecules; nepal; nepjol.info/index.php/njb; njb; non; number; olive; organic; parameters; pef; phenolic; plant; polyphenols; present; pressure; pressurized; process; processing; product; pulses; purification; quality; rate; recovery; red; review; sample; sci; seeds; selectivity; separation; sfe; society; solid; solvent; solvent extraction; sources; soxhlet; study; supercritical; surname; system; tea; technique; technologies; temperature; thermal; time; total; treatment; type; uae; ultrafiltration; ultrasound; use; vol; water; wine
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        item: #31 of 115
          id: njb-204
      author: Shrestha, Dhiraj; Bhattachan, Balkrishna
       title: Nipah Virus (NiV) Infection: Is Nepal Prepared for the Possible Outbreak?
        date: 2019-01-15
       words: 2696
      flesch: 59
     summary: Thus, the territorial habitat of these bats is the high risk zone of NiV outbreak. 23 Dawes BE, Kalveram B, Ikegami T, Juelich T, Smith JK, Zhang L, Park A, Lee B, Komeno T, Furuta Y, Freiberg AN: Favipiravir (T-705) protects against Nipah virus infection in the hamster model.
    keywords: available; bats; bhattachan; biotechnology; blueprint; cases; disease; distribution; doi; encephalitis; family; health; high; host; human; ifn; india; infection; journal; model; mortality; natural; nepal; nepjol.info/index.php/njb; nipah; nipah virus; niv; njb; organization; outbreak; pigs; pmid; possible; prevention; protection; proteins; r&d; research; respiratory; risk; shrestha; signaling; society; symptoms; threat; transmission; vaccines; virol; virus; vol; world
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        item: #32 of 115
          id: njb-205
      author: Thapa, Christina; Shakya, Pallavi; Shrestha, Rabina; Pal, Sushovita; Manandhar, Prakash
       title: Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB
        date: 2019-01-15
       words: 3576
      flesch: 50
     summary: ©NJB, Biotechnology Society of Nepal 62 Nepjol.info/index.php/njb Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB Christina Thapa¹, Pallavi Shakya¹, Rabina Shrestha¹, Sushovita Pal¹, Prakash Manandhar² Department of Biotechnology, SANN International College, Purbanchal University, Nepal1 Department of Microbiology, St. Xavier’s College, Nepal2 Abstract Polyhydroxybutyrates (PHBs) are energy reserves synthesized by different micro-organisms such as Alcaligenes, Pseudomonas, Staphylococcus, Algae, in excess of carbon and limitation of nutrients like nitrogen. The micro-organisms for this purpose were isolated from 4 different soil samples and screened for PHB production.
    keywords: accumulation; agar; analysis; arthrobacter; bacillus; bacteria; biotechnology; black; broth; carbon; characterization; concentration; conditions; culture; different; extraction; figure; ftir; glucose; growth; hours; incubation; isolates; isolation; journal; luteus; media; micrococcus; morphological; nacl; nepal; nepjol.info/index.php/njb; njb; optimization; pasteurii; phb; plastics; positive; production; research; samples; showing; society; source; species; spp; staining; strains; sudan; test; vol
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        item: #33 of 115
          id: njb-21
      author: Sah, Sunil Kumar; Yadav, Naval Kishor; Kurmi, Roshan; Rauniyar, Ramanuj; Manandhar, Krishan Das; Gupta, Birendra Prasad
       title: Pattern of Cancer in Nepal from 2003 to 2011
        date: 2016-12-31
       words: 2711
      flesch: 64
     summary: The total number of cancer cases were increasing from 2003 to 2011 and it become double in 2011.. Out of 75 district of Nepal, more number of cancer cases was found in Kathmandu, Sunsari, Morang, Chitwan, Lalitpur, Jhapa, Kaski, Nawalparasi, Rupendehi and Kavrepalchowk in 2010.
    keywords: age; biotechnology; breast; cancer; cancer cases; cases; chitwan; common; diagnosis; distribution; examination; female; figure; group; hospital; iarc; journal; kathmandu; kurmi; lung; male; nepal; nepjol.info/index.php/njb; njb; number; religion; society; study; table; total; vol; years
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        item: #34 of 115
          id: njb-212
      author: Bhattachan, Balkrishna; Rai, Ganesh; Munankarmi, Nabin Narayan; Sherchand, Jeevan Bahadur
       title: Cyclospora cayetanensis: An Infestation among Diarrheal Children in Kathmandu Valley, Nepal
        date: 2019-01-15
       words: 2267
      flesch: 63
     summary: ©NJB, Biotechnology Society of Nepal 16 Nepjol.info/index.php/njb Cyclospora cayetanensis: Orozco-Mosqueda GE, Martı´nez-Loya OA, and Ortega YR: Cyclospora cayetanensis in a Pediatric Hospital in Morelia, Mexico.
    keywords: age; august; biotechnology; cayetanensis; children; cross; cryptosporidium; cyclospora; diarrheal; female; higher; highest; hospital; infection; intestinal; journal; june; kanti; kathmandu; med; medicine; nepal; old; parasites; patients; prevalence; rate; research; samples; september; society; stool; study; table; total; water; year
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        item: #35 of 115
          id: njb-213
      author: Nepal, Pragya; Singh, Minu; Baniya, Amina; Singh, Sushma; Sainju, Hari Krishna; Shrestha, Rajani
       title: Comparative Antioxidant, Antimicrobial and Phytochemical Assesments of Leaves of Desmostachya bipinnata L. Stapf, Hordeum vulgare L. and Drepanostachyum falcatum (Nees) Keng f.
        date: 2019-01-15
       words: 5084
      flesch: 51
     summary: Plant extracts used in different … H.vulgare D.bipinnata D.falcatum 0 5 10 15 20 25 Pure 2:01 1:01Z o n e o f In h ib it io n ( in m m ) Plant extracts used in different concentrations H.vulgare D.bipinnata D.falcatum 0 5 10 15 20 25 pure 2:01 1:01 Z o n e o f In h ib it io n (i n m m )
    keywords: + +; absorbance; acid; activity; alkaloids; analysis; antifungal; antimicrobial; antioxidant; assay; aureus; bacteria; biotechnology; bipinnata; compounds; concentration; coumarins; desmostachya; different; dpph; drepanostachyum; drepanostachyum falcatum; extracts; falcatum; figure; glycosides; graph; hordeum; hordeum vulgare; inhibition; journal; keng; kumar; medicinal; methanol; nees; nepal; nepjol.info/index.php/njb; njb; phytochemical; plants; positive; presence; present; pure; radical; religious; result; salmonella; sample; saponins; scavenging; screening; society; solution; sterols; table; test; use; vol; vulgare; zoi; zone
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        item: #36 of 115
          id: njb-214
      author: Saiju, Hari Krishna; Bajracharya, Abhishesh; Rajbahak, Brishav; Ghimire, Stuti
       title: Comparative Study of Growth Statistics of Two Species of Paulownia and Optimization of Rooting Methods
        date: 2019-01-15
       words: 3267
      flesch: 68
     summary: Keywords: Paulownia tomentosa, Paulownia fortuneii, growth comparison, in-vitro rooting, sand- rooting, nodal culture *Corresponding Author Email: hk.saiju@aitm.edu.np Introduction Paulownia is a fast-growing, woody tree native to the forests of China [1]. Steud and Paulownia fortuneii (Seem.)
    keywords: average; average root; biotechnology; comparison; concentrations; culture; density; development; figure; fortuneii; growth; higher; incubation; l naa; length; medium; naa; nepal; number; optimization; paulownia; plants; rate; room; root; rooting; samples; sand; species; study; table; tomentosa; total; vessels; week; wood
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        item: #37 of 115
          id: njb-22
      author: Thapa, Nita; Thapa, Pratiksha; Bhandari, Jay; Niraula, Prasodhan; Shrestha, Nikita; Shrestha, Bhupal G
       title: Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus
        date: 2016-12-31
       words: 3568
      flesch: 57
     summary: ©NJB, Biotechnology Society of Nepal 52 Nepjol.info/index.php/njb IC50 value of ascorbic acid = 5.62µg/ml IC50 value of Artocarpus heterophyllus = 8.33µg/ml IC50 value of ascorbic acid = 12.96µg/ml IC50 value of Artocarpus heterophyllus = 29.29µg/ml IC50 value of Ascorbic acid=5.27µg/ml IC50 value of Artocarpus heterophyllus=10.69µg/ml Figure 2: A, B and C represents antioxidant activity of methanol, ethyl acetate, aqueous methanol respectively. topic of antimicrobial plant extracts as they believe that these phytochemicals will find their way as alternate to antimicrobial drugs as those prescribed by physicians. Further, antimicrobial activity was assessed through disc diffusion method with six pathological bacteria and two fungi strains in four different concentrations of plant extract.
    keywords: 1ml; 200mg; acetate; acid; activity; antimicrobial; antioxidant; aqueous; artocarpus; ascorbic; biotechnology; cancer; coumarin; dpph; ethyl; extract; extraction; flavus; heterophyllus; ic50; inhibition; journal; leaf; medicinal; methanol; nepal; nepjol.info/index.php/njb; njb; phytochemical; plant; presence; rhizopus; sample; saponin; shrestha; society; solution; solvent; standard; study; subtilis; table; test; thapa; traditional; use; value; vol; yield; zoi
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        item: #38 of 115
          id: njb-224
      author: Balzer, Anna; Shah, Sujit; Kumar Ale, Yam; Adhikari, Dipti; Niroula, Sanjit; Mandal, Jawaharlal; Bahadur Pun, Amar; Poudel, Krishna; Bhantana, Parashuram; Timsina, Govinda; Karn, Ravindra; Karki, Sujwal
       title: Development of Effective Protocol for Four Varieties of Large Cardamom
        date: 2022-07-30
       words: 3871
      flesch: 65
     summary: ©NJB, BSN 3 Table 1: Growth patterns of Dambarsai, Golsai, Sikkimae, and Ramsai cardamom varieties in response to differing cytokinin concentration in micropropagation. Table 2: Growth ranking for optimal shooting and rooting of Dambarsai, Golsai, Sikkimae, and Ramsai cardamom varieties in response to differing cytokinin concentration in micropropagation.
    keywords: 0.5mg; 1mg; acclimatization; amomum; assay; author; bap; biotechnol; cardamom; cocopeat; concentration; culture; cytokinin; dambarsai; days; development; different; disease; field; golsai; growth; high; iba; l bap; l iba; large; length; media; micropropagation; moss; nepal; number; optimal; plant; production; protocol; quality; ramsai; ranking; research; root; roxb; sawdust; shoot; sikkimae; soil; study; substrate; subulatum; table; tissue; transfer; varieties; variety
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        item: #39 of 115
          id: njb-225
      author: Prasai, Shristi; Shrestha, Puja; Pandey, Sriniwas; Adhikari, Ishika; Gurung, Srijana; Prajapati, Kamil
       title: Microbial Quality Assessment of Raw Freshwater Fish Sold in Local Markets of Kathmandu Valley
        date: 2022-07-30
       words: 4746
      flesch: 64
     summary: It was then serially diluted up to a dilution of 10-5. Bacteriological analysis of fish samples For total plate count (TPC), 0.1ml sample from 10-3 and 10-5 dilutions were taken and spread plating was done on PCA agar. Average TPC of fish samples Sample Organs (cfu/gm in average) Skin Gills Intestine Average LR 6.5 x 106 8.2 x 107 8.05 x 107 5.6 x 107 CC 1.23 x 107 2.96 x 107 8.03 x 107 4.1 x 107 CB 1.2 x 107 6.6 x 107 2.3 x 108 1.02 x 108 Average 1.02 x 107 5.9 x 107 1.3 x 108 Note: LR = Labeo rohita, CC = Cyprinus carpio and CB = Clarias batrachus In this study, 9 fish sample of 3 different varieties, L. rohita (LR), C. carpio (CC) and C. batrachus (CB) were analyzed for its microbial quality and antimicrobial susceptibility testing was done for the isolated strain.
    keywords: aureus; average; bacteria; batrachus; c. carpio; carpio; cfu; cholerae; coli; coliform; cons; contaminated; count; cyprinus; different; fecal; fish; fishes; food; freshwater; gills; gram; highest; intestine; isolated; kathmandu; labeo; load; markets; microbial; nepal; paratyphi; pathogenic; presence; present; quality; raw; rohita; salmonella; samples; sci; skin; spp; staphylococcus; study; table; tcc; total; tpc; typhi; vibrio
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        item: #40 of 115
          id: njb-226
      author: Habiba Akter, Umme; Begum, Fatema; Islam, M. R.; Nahar Prinky, Jannatun; Khatun, Mst. Rehena
       title: Occurrence of Purple Blotch Disease Associated with Selected Garlic Varieties and its Management Through Bio-Agent, Botanicals and Fungicides
        date: 2022-07-30
       words: 8468
      flesch: 65
     summary: The current research work was aimed to assess the occurrence of purple blotch disease incidence and severity of selected garlic varieties, to isolate and identify purple blotch disease and pathogenicity test of Alternaria porri, and its management using bio-agent, plant extracts and fungicides. BARI Rashun-3 showed the highest disease incidence (40.00%) and severity (92.00%) of purple blotch disease.
    keywords: agar; agent; agricultural; akter; allium; alternaria; alternaria porri; bangladesh; bari; bau; bau1; bau2; bio; biotechnol; black; blotch; blotch disease; botanicals; brown; bsn; camara; colony; color; condition; control; correlation; das; data; days; different; disease; disease incidence; disease severity; effective; ellis; evaluation; extract; fluffy; fresh; fungicides; garlic; growth; harzianum; identification; incidence; indian; inoculation; int; islam; isolates; lantana; leaf; leaves; lesions; level; lind; local; lowest; management; maximum; media; morphological; nepal; njb; onion; pathogenicity; pathogens; pda; plant; porri; production; purple; purple blotch; rashun-3; regular; research; results; sci; severity; similar; soil; studies; symptoms; table; test; treatment; trichoderma; university; varieties; variety; water; weight; white; yield
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        item: #41 of 115
          id: njb-227
      author: Kumar Shrestha, Bijay; Dhungana, Bidhya; Shakya, Jenish; Shrestha, Romika; Chauhan, Sujata
       title: Phytochemical and Antimicrobial Screening of Bark Extract of Shorea robusta (Sal)
        date: 2022-07-30
       words: 4546
      flesch: 51
     summary: Antimicrobial Screening of bark extract of S. robusta against E. coli Figure 3: Antimicrobial susceptibility test by S. robusta extract. In one study, S. robusta extract exhibited antibacterial activity against different clinical pathogens
    keywords: acid; activity; agar; alkaloids; anthraquinone; antibacterial; antimicrobial; aureus; ayurvedic; bacterial; bark; bark extract; biotechnol; bsn; campus; central; clinical; coli; color; concentration; cultural; culture; development; different; diseases; doi; drug; ethanolic; ethanolic bark; ethanolic extract; extract; flavonoids; gaertn; growth; himedia; india; infections; information; inhibition; inhibitory; inoculated; journal; medicinal; minimum; nepal; njb; pathogens; phlobatannins; phytochemical; plant; plates; presence; present; proteins; research; resistance; robusta; robusta bark; s. robusta; sample; screening; shorea; shorea robusta; shrestha; solution; steroids; strains; study; suspension; table; tannins; technology; terpenoids; test; traditional; zone
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        item: #42 of 115
          id: njb-228
      author: Bajgain, Abhishek; Dev Neupane, Bashu; Sarraf, Diwakar; Karmacharya, Jwalant; Ranjitkar, Saksham; Shrestha, Rajan; Gyawali, Rajendra
       title: Quality Evaluation of Apis laboriosa and Apis mellifera Honey Collected from Bagmati Province, Nepal
        date: 2022-07-30
       words: 4502
      flesch: 65
     summary: Various values of pH shown by different honey samples are tabulated down in Table 4. Honey samples of Apis laboriosa and Apis mellifera were collected during spring of 2019 & 2021 and autumn 2021 from the Bagmati province, Nepal.
    keywords: + +; absorbance; acid; anti; apis; autumn; bajgain; biotechnol; bsn; colour; concentration; content; different; dpph; figure; flow; honey; honey samples; index; instrument; laboriosa; manufacturer; moisture; nectar; nepal; njb; optical; oxidant; phenolic; positive; potency; ppm; properties; quercetin; refractive; rheological; rotation; rpm; s.n; samples; sand; specific; spring; standard; table; test; tfc; time; total; tpc; viscosity; water
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        item: #43 of 115
          id: njb-229
      author: Saud, Sarada; Agrawal, Ashwani; Pokhrel, Soniya; Subedi, Sushma; Shrestha, Sanjit; Man Amatya, Niroj
       title: Screening of Carbapenem Resistance Klebsiella pneumoniae and its MIC against Imipenem
        date: 2022-07-30
       words: 3321
      flesch: 44
     summary: Therefore, this cross-sectional study was conducted in B & B Hospital, Lalitpur to screen the distribution of carbapenem resistance K. pneumoniae through ertapenem and to assess the minimum inhibitory concentration of imipenem for screened carbapenem resistance K. pneumoniae. Shilpa K, Thomas R, Ramyashree A. Isolation and Antimicrobial sensitivity pattern of Klebsiella pneumoniae from sputum samples in a tertiary care hospital.
    keywords: antibiotic; antimicrobial; bacterium; biotechnol; blood; bsn; carbapenem; carbapenemase; ceftriaxone; ciprofloxacin; clinical; clsi; control; crkp; different; disc; enterobacteriaceae; ertapenem; european; gentamicin; global; health; hospital; imipenem; india; infection; intermediate; isolates; klebsiella; mic; microbiology; nepal; njb; non; number; pathogen; patients; pneumoniae; range; rate; resistance; samples; saud; screening; sensitive; study; surveillance; susceptibility; susceptible; table; test
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        item: #44 of 115
          id: njb-23
      author: Sharma, Manisha; Pant, Narayan Dutt; Pandey, Pratikshya
       title: Prevalence of Non-Albicans Candida Among the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal
        date: 2016-12-31
       words: 2488
      flesch: 49
     summary: Among them, Candida glabrata, Candida parapsilosis and Candida tropicalis are non-albicans Candida of increasing clinical significance [5]. Abstract The main objective of this study was to determine the prevalence of non-albicans Candida among the patients attending a tertiary care hospital in Kathmandu, Nepal.
    keywords: albicans; albicans candida; biotechnology; candida; candida spp; care; clinical; deorukhkar; fungal; glabrata; hospital; identification; immunocompromised; infection; isolated; isolation; kathmandu; nepal; non; patients; prevalence; rate; samples; secretion; species; spp; sputum; study; swab; test; total; tropicalis; urine; vaginal
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        item: #45 of 115
          id: njb-239
      author:  Kumar Shrestha, Deepak; Kumar Sapkota, Basanta; Prasad Sharma, Krishna
       title: Assessment of Phytochemicals, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Selected Nepalese Medicinal Plants
        date: 2022-12-31
       words: 4837
      flesch: 56
     summary: Polyphenols are the strong antioxidant in plant extracts [11]. This test is a straightforward, popular, and widely accepted method to assess the antioxidant potential of plant extracts.
    keywords: + +; absorbance; acid; activities; activity; adhatoda; aegle; alkaloids; analysis; antioxidant; arbortristis; ascorbic; biotechnol; bsn; calibration; chloride; ciocalteu; concentration; content; crateva; curve; different; dioica; dpph; evaluation; extract; flavonoid; folin; free; gae; gallic; highest; ic50; journal; justicia; lowest; marmelos; mean; medicinal; metabolites; methanolic; method; natural; nepal; njb; nyctanthes; oxidative; phenolic; phytochemical; plants; polyphenols; present; quercetin; radical; reagent; research; results; sample; scavenging; shrestha; sodium; solution; standard; study; table; test; total; tpc; unilocularis; urtica; value; weight
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        item: #46 of 115
          id: njb-24
      author: Shrestha, Kushal; Shrestha, Smita; Khatiwada, Saroj; Acharya, Bishu; Manandhar, Sulochana; Pokharel, Rohit Kumar
       title: Multiplex Ligation Dependent Probe Amplification Based Mutation Analysis of Dystrophin Gene in Nepalese Patients with Duchenne Muscular Dystrophy
        date: 2016-12-31
       words: 3087
      flesch: 58
     summary: A commercial MLPA kit (MRC Holland) with probes of P034 (DMD exons 1-10, 21-30, 41-50, and 61-70) and P035 (DMD exons 11-20, 31-40, 51-60, and 71-79) was used to detect DMD deletion/duplication in DMD patients according to the manufacturer’s recommended protocol (Amsterdam, Netherlands). Wang X, Wang Z, Yan M, Huang S, Chen TJ, Zhong N: Similarity of DMD gene deletion and duplication in the Chinese patients compared to global populations.
    keywords: age; amplification; analysis; assay; biotechnology; bmd; cases; clinical; deletion; dependent; detection; diagnosis; dmd; dna; duchenne; duplications; dystrophin; dystrophin gene; exon; exonic; gene; genetic; journal; level; ligation; mlpa; multiplex; muscular; mutation; nepal; nepalese; nepjol.info/index.php/njb; njb; number; p034; p035; parents; patients; probe; regions; samples; shrestha; society; study; test; tribhuvan; university; vol; years
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        item: #47 of 115
          id: njb-240
      author: Gyawali, Rajendra; Paudel, Aparna; Lamsal, Babita; Bhatta, Pranisha; Maharjan, Albina; Khaitu, Nina; Shrestha, Rajan
       title: Comparative Study on Antioxidant Activity of Propolis of Apis mellifera from Different Regions of Nepal
        date: 2022-12-31
       words: 2987
      flesch: 53
     summary: Propolis samples Total Phenolic Content Nepal J Biotechnol. It is concluded that there is no direct correlation on total antioxidant property of propolis with their total phenolic and total flavonoid content among collected samples.
    keywords: absorbance; acid; activity; antibacterial; antioxidant; assay; bee; biological; biotechnol; capacity; chemical; collected; composition; compounds; content; different; doi; dpph; equivalent; extract; figure; flavonoid; free; gae; gallic; gram; gyawali; highest; ic50; journal; method; nepal; nepalese; njb; origin; phenolic; plant; properties; property; propolis; quercetin; radical; research; samples; scavenging; shrestha; spl; standard; study; total; value
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        item: #48 of 115
          id: njb-241
      author: Rehena, M. K.; Aminuzzaman, F. M.; Ashrafi, M. L.; Chowdhury, M.S.M.; Habiba, U. A.; Nazifa, Z.; Ahmed, M.
       title: Efficacy of Ethanol Extract of Botanicals in Controlling Wheat Blast Fungus Magnaporthe oryzae triticum in vitro
        date: 2022-12-31
       words: 4826
      flesch: 61
     summary: The effect of plant extract was calculated as percent growth inhibition using the following formula as adopted by [22, 23]. Effects of plant extracts on controlling wheat blast disease caused by Magnaporthe oryzae Pathotype triticum in Bangladesh.
    keywords: 1:0.25; allamanda; allium; aloe; aloe vera; azadirachta; bangladesh; black; blast; botanicals; cathartica; cepa; colony; concentration; control; culture; cumin; curcuma; dai; days; different; disease; ethanol; extracts; food; garlic; growth; highest; indica; inhibition; leaf; longa; magnaporthe; media; mot; mycelial; nepal; nigella; officinale; oryzae; pathogen; pda; plant; plates; radial; regular; research; rice; sativa; sativum; seed; table; triticum; vera; vitro; wheat; white; zingiber
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        item: #49 of 115
          id: njb-242
      author: Chinenye Onwubiko, Nwakuche; Ifeanyi Uguru, Michael; Ovute Chimdi, Grace
       title: Molecular Genetic Diversity in Bambara groundnut [Vigna subterranea (L.) Verdc] Assessed by Microsatellite Markers
        date: 2022-12-31
       words: 3870
      flesch: 56
     summary: In fact, there was a report from a previous study on a similar trend of association between Bambara groundnut accessions collected from different geographical areas, and in that study, it was concluded that these accessions were either related, or the genotypes were the same (22). 2 0 2 2 D e c ; 1 0 (2): 85-90 Research article DOI: https://doi.org/10.54796/njb.v10i2.242 ©NJB, BSN 85 Molecular Genetic Diversity in Bambara groundnut [Vigna subterranea (L.) Verdc]
    keywords: accessions; analysis; areas; arithmetic; bambara; bambara groundnut; biotechnol; bsn; characters; cluster; crop; dendrogram; different; diversity; dna; gel; genetic; genetic diversity; genotypes; geographical; groundnut; groundnut accessions; group; improvement; jaccard; journal; landraces; level; markers; mean; minutes; molecular; morphological; nepal; nigeria; njb; onwubiko; origin; plant; polymorphism; report; result; selection; similarity; species; ssr; study; subterranea; supernatant; togo; tubes; tvsu; variation; verdc; vigna; vigna subterranea
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        item: #50 of 115
          id: njb-243
      author: Aththanayake, A.M.K.C.B.; Rathnayake, I.V.N.; Deeyamulla, M.P.
       title: Detoxification and Removal of Hexavalent Chromium in Aquatic Systems: Applications of Bioremediation
        date: 2022-12-31
       words: 14927
      flesch: 43
     summary: Adsorption is widely used for chromium removal in wastewater, consisting of Nepal J Biotechnol. This study also indicates 65% of chromium removal from tannery effluent and 85% of Cr(VI) removal from the synthetic medium at pH 6 within 07 days.
    keywords: accumulation; acidic; acinetobacter; adsorbent; adsorption; adsorption cr(vi; agents; air; algae; application; approach; apr; aquatic; aqueous; arthrobacter; aspergillus; aththanayake; attachment; aug; authorities; available; bacillus; bacterial; beads; biodegrad; biodeterior; biofilm; biological; biomass; bioreduction; bioremediation; bioresour; biosorption; biotechnol; bsn; calcium; cancer; carbon; cell; cellulosimicrobium; cerevisiae; changes; characterization; chemical; chemosphere; chen; chlorella; chromate; chrome; chromium; chromium reduction; chromium removal; chromium(vi; coli; community; components; compounds; concentration; conditions; consortia; contaminated; cost; cr(iii; cr(vi; crb; crit; damages; das; days; dec; desalination; detoxification; different; discharge; disposal; editors; effective; effects; efficient; effluent; electron; electroplating; eng; eng j; environ; environ sci; environmental; eps; escherichia; et al; exchange; exposure; factors; feb; filtration; formation; forms; free; friendly; fungal; fungi; genotoxic; glass; green; growth; hazard; health; heavy; hexavalent; hexavalent chromium; high; higher; hrs; https://linkinghub.elsevier.com/retrieve/pii/; husk; immobilized; industrial; industry; inhibition; initial; int j; international; internet; isolated; j environ; j hazard; jan; jul; jun; kelaniya; kumar; leather; lett; level; liu; living; low; mar; marine; mater; materials; mechanisms; membrane; metal; methods; microbiol; microorganisms; mixed; mobility; montmorillonite; nanocomposite; nanoparticles; natural; nature; nepal; nepal j; new; njb; nov; occupational; oct; optimization; oxidation; oxide; park; physical; physiological; phytoremediation; pigment; planktonic; plants; point; pollut; pollutants; potential; presence; preservation; process; processes; pseudomonas; range; raw; reduction; reduction cr(vi; remediate; remediation; removal; requirements; res; research; resins; resistance; response; review; saccharomyces; saha; scale; sci; sep; shewanella; silva; site; skin; soil; solubility; solutions; sorokiniana; sources; species; springer; stainless; staphylococcus; states; steel; strain; stress; studies; study; subtilis; surface; synthetic; synthetic wastewater; system; table; tannery; tanning; techniques; technologies; technology; temperature; textile; time; tolerance; toxic; toxicity; toxicol; treatment; trichoderma; trivalent; type; ulva; uptake; vulgaris; wang; wastes; wastewater; wide; wood; yeast; zhang
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        item: #51 of 115
          id: njb-25
      author: Amgai, Resham Babu; Prasai, Hari Kumar; Pandey, Yama Raj
       title: Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation
        date: 2016-12-31
       words: 2251
      flesch: 64
     summary: MS basal media+4mg/l BAP+0.2mg/l IAA don’t produce any shooting directly from immature shoot with single bud (Table 6). 3. Sharma S, Singh B, Rani G, Zaidi AA, Hallan V, Nagpal A, Virk GS: Production of Indian citrus ringspot virus free plants of Kinnow employing chemotherapy coupled with shoot tip grafting.
    keywords: agriculture; bap; basal; biotechnology; bud; callusing; citrus; composition; culture; different; explant; flower; grafting; highest; iaa; immature; incubation; mandarin; mean; media; micro; nepal; observed; orange; shooting; single; table; test; tip
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        item: #52 of 115
          id: njb-26
      author: Kurmi, Roshan; Rauniyar, Ramanuj; Manandhar, Krishna Das; Gupta, Birendra Prasad
       title: Evaluation of the XpertMTB/RIF for the Diagnosis of Pulmonary Tuberculosis Among the Patients Attending DOTS Center Parsa District of Nepal
        date: 2016-12-31
       words: 4235
      flesch: 55
     summary: [1]. DOTS program was initiated in April 2001 with a goal to diagnose and cure TB patients which has treated more than 20 thousand patients till date [2]. Gene Xpert MTB RIF Pattern in TB patient and MDR Pattern in TB Patients are shown in Figure 2 and Figure 3 respectively.
    keywords: analysis; assay; biotechnology; cases; centres; chest; clinical; combined; cough; culture; development; diagnosis; district; dots; figure; genexpert; government; health; higher; hiv; journal; kurmi; low; mdr; microscopy; mtb; national; negative; nepal; nepjol.info/index.php/njb; njb; number; parsa; patients; positive; possible; predictive; prevalence; program; pulmonary; rapid; region; resistant; results; rif; sensitivity; similar; smear; society; specificity; sputum; study; table; test; treatment; tuberculosis; uncentrifuged; value; vol
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        item: #53 of 115
          id: njb-27
      author: Shrestha, Rupendra; Khadka, Sundar; Wagle, Susbin Raj; Sapkota, Alisha
       title: Detection of Latent HIV-1 Infection and Drug Resistant Mutation Testing in Nepal: HIV-1 env V3 DNA Sequence and RT Gene (M184V) Mutation
        date: 2016-12-31
       words: 4705
      flesch: 59
     summary: In addition, there have been no systematic studies of HIV-1 resistance mutation in Nepalese population. Although the study shows negative for the M184V resistance mutation, screening of various panels of drug resistance mutations should be performed in recently infected HIV- 1 patients for planning the effective ART strategy.
    keywords: 3tc; aids; amplification; amplified; antiretroviral; art; azt; biotechnology; cviaii; detection; digestion; dna; drug; drug resistance; env; gel; gene; genotypic; high; hiv-1; human; infected; infection; inhibitors; journal; lamivudine; latent; limit; m184v; m184v mutation; minutes; molecular; mutant; mutation; negative; nepal; nepjol.info/index.php/njb; nested; nfv; njb; patients; pcr; positive; present; primers; product; proviral; proviral dna; reaction; research; resistance; resistance mutation; restriction; results; reverse; rfda; rna; samples; sec; sequence; shrestha; size; society; specific; study; table; testing; therapy; transcriptase; treatment; type; v3 dna; variants; virus; vol; wild; ° c
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        item: #54 of 115
          id: njb-274
      author: NJB, Nepal Journal of Biotechnology
       title: Effect of Coconut Water and GA3 Concentrations on in vitro Clonal Propagation of Potato Cultivars from Nepal
        date: 2023-07-31
       words: 5563
      flesch: 67
     summary: 1.16 CW 20 mL/L 9.5cd ± 0.5 5.67a ± 1.15 7bc ± 1 9.33ab ± 0.58 55c ± 6.24 1.02b ± 0.11 Cardinal 0 5.5b ± 0.46 10.03a ± 0.31 2.67a ± 0.58 7.67a ±1.53 0.73b ± 0.10 10.46a ± 1.57 9.84a ± 0.68 0.1 7.93a ± 0.86
    keywords: 200c; abm; agar; agent; basal; biomass; biotechnol; bsn; cardinal; cbm; clarigel; coconut; combination; comparison; composition; concentrations; culture; dev; development; different; dry; g+c; ga3; gellan; gelrite; growth; gum; hairs; himedia; internode; jam; janak; journal; kafle; leaf; length; media; medium; meristem; multiplication; nepal; njb; nodal; nodes; number; performance; plant; plantlets; potato; propagation; rate; regulators; results; root; root length; shoot; size; sprouts; study; survival; tip; tissue; unchanged; values; varieties; variety; water
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        item: #55 of 115
          id: njb-28
      author: Amgai, Resham Babu; Pantha, Sumitra; Bhatta, Madan Raj
       title: Characterization of Nepalese Barley Gene Pool for Leaf Rust Resistance
        date: 2016-12-31
       words: 2914
      flesch: 67
     summary: Mammadov JA, Brooks WS, Griffey CA, Saghai Maroof MA: Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7. Identification of particular rust resistance gene and its incorporation is the only option for the development of barley varieties for leaf rust rust resistance.
    keywords: 5hs; accessions; barley; biotechnology; bonus; characteristics; collection; disease; gene; genetic; genotype; germplasm; gr-25; jau; jc#49; journal; lalitpur; landraces; leaf; leaf rust; local; major; markers; molecular; nb-1003; nepal; nepalese; nepjol.info/index.php/njb; njb; pcr; plant; presence; puccinia; qblr; qtl; research; resistance; rph2; rph7; rust; rust resistance; screening; society; solu; ssr; susceptible; table; uwa; variation; vol
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        item: #56 of 115
          id: njb-29
      author: Maharjan, Mahendra; Mandal, Swati; Madhubala, Rentala
       title: Assessing the Role of Potential Biomarkers in Antimony Susceptible and Resistant Clinical Isolates of L. donovani from India
        date: 2016-12-31
       words: 6676
      flesch: 54
     summary: Comparison of MRPA gene expression in SAG-S versus SAG-R field isolates was done to verify if there is any correlation between MRPA gene expression and SAG susceptibility profile of the clinical isolates. MRPA gene expression in SAG-S versus SAG-R field isolates showed correlation between MRPA gene expression and SAG susceptibility profile of the clinical isolates.
    keywords: abc; ag83; amastigotes; amplification; analysis; antimony; azar; biotechnology; blot; clinical; clinical isolates; control; digested; dna; donovani; drug; earlier; expression; field; figure; fold; gene; hindiii; india; intracellular; isolates; journal; kala; leishmania; leishmaniasis; levels; maharjan; mc4; mc8; mc9; mrpa; nepal; nepjol.info/index.php/njb; njb; nr3a; number; odc; ouellette; patients; pcr; pkdl; present; probe; promastigotes; resistant; resistant isolates; rk1; rmp142; rmp155; rmp240; rmp8; role; sag; sbiii; sensitive; society; southern; specific; strains; study; thiol; time; total; transporter; trypanothione; vol; -gcs
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        item: #57 of 115
          id: njb-3
      author: Khadka, Sundar; Kandel, Samikshya; Pandit, Roshan; Manjhi, Rosham; Dhital, Subhash; Baniya, Jagat Bahadur; Mishra, Shravan Kumar; Mahato, Raj Kumar
       title: Sero-prevalance of Cryptococcal Antigenemia in HIV Positive Individual having CD4 Counts 
        date: 2021-12-30
       words: 2550
      flesch: 56
     summary: The study concludes higher prevalence of Cryptococcal antigenemia among HIV infected individuals and recommends Cryptococcal antigen screening to be made mandatory in HIV positive patients having CD4 T cells count below 100/µl. All subjects enrolled in this study were HIV positive patients having CD4 T cells counts less than 100 cells/mm3.
    keywords: agglutination; aids; antigen; antigenemia; assay; cd4; cells; count; crag; cryptococcal; data; deficiency; detection; female; flow; health; higher; hiv; immunocompromised; individuals; infection; journal; laboratory; latex; meningitis; mm3; national; neoformans; nepal; opportunistic; patients; people; positive; prevalence; rate; screening; study; test; total
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        item: #58 of 115
          id: njb-30
      author: Aryal, Sagar; Karki, Gaurab; Pandey, Sunil
       title: Microbial Diversity in Freshwater and Marine Environment
        date: 2015-12-30
       words: 1781
      flesch: 53
     summary: Nominal cell counts of >105 cells per ml in surface sea water [12, 13] predict that the oceans harbor 3.6 X 1029 microbial cells with a total cellular carbon content of 3 X 1017g Freshwater Microbial Diversity Freshwater and marine environments differ in many ways including salinity, average temperature, depth, and nutrient content, but both provide many excellent habitats for microorganisms.
    keywords: bacteria; benjamin; benthic; biotechnology; carbon; cells; cummings; cyanobacteria; depth; diversity; earth; ecology; environment; freshwater; high; life; light; limnetic; marine; meters; microbial; microbiology; microorganisms; nepal; numbers; nutrient; ocean; organic; oxygen; phototrophs; primary; prokaryotic; sea; species; surface; water; zone
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        item: #59 of 115
          id: njb-31
      author: Pant, Narayan Dutt; Sharma, Manisha; Khatiwada, Saroj
       title: Fasciolopsis buski Vomited Out by a Child; The First Case Reported from Nepal
        date: 2015-12-30
       words: 1107
      flesch: 50
     summary: ISSN 2467-9319 (online) CASE STUDY ©NJB, Biotechnology Society of Nepal 66 Nepjol.info/index.php/njb Fasciolopsis buski Vomited Out by a Child; The First Case Reported from Nepal Narayan Dutt Pant1*, Manisha Sharma2, Saroj Khatiwada3 1Department of Microbiology Grande International Hospital, Dhapasi, Kathmandu, Nepal 2Department of Microbiology, Kathmandu medical college, Kathmandu, Nepal 3CIST College, Kathmandu, Nepal Abstract Live adult worms of Fasciolopsis buski are rarely seen in humans except in autopsy. Trop Dis Bull 1994, 91:r126-r150. 4. Mahajan RK, Duggal S, Biswas NK, Duggal N, Hans C: A finding of live Fasciolopsis buski in an ileostomy opening.
    keywords: adult; antihelminthic; biotechnology; buski; case; child; common; diarrhea; fasciolopsis; gastrointestinal; history; humans; india; infection; kathmandu; live; nepal; patient; report; treatment; water; worms
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        item: #60 of 115
          id: njb-32
      author: Kusi, Meena; Shrestha, Kanti; Malla, Rajani
       title: Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu
        date: 2015-12-30
       words: 4132
      flesch: 54
     summary: [21- 23] Brine Shrimp Lethality Assay: Crude plant extract was prepared in three different concentrations of 1000ppm, 100ppm and 10ppm. Viscum album in Nepal has been used as a medicinal herb since very long.
    keywords: acacia; activity; aerial; album; alkaloids; analysis; antibacterial; antioxidant; assay; associated; atcc; bioactive; biotechnology; brine; cardiac; catechu; compounds; concentration; control; crude; dpph; dry; ec50; extract; ferric; flavonoids; frap; free; glycosides; gram; inhibition; journal; khusi; known; larvae; lc50; linn; medicinal; medicine; methanolic; mistletoe; nepal; nepjol.info/index.php/njb; njb; parts; percentage; phytochemical; plant; positive; presence; radical; reagent; results; saponins; scavenging; science; screening; shrimp; significant; society; standard; study; table; tannins; test; toxicity; use; value; viscum; viscum album; vol
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        item: #61 of 115
          id: njb-33
      author: Awasthi, Tek Raj; Pant, Narayan Dutt; Dahal, Puspa Raj
       title: Prevalence of multidrug resistant bacteria in causing community acquired urinary tract infection among the patients attending outpatient department of Seti Zonal Hospital, Dhangadi, Nepal
        date: 2015-12-30
       words: 2988
      flesch: 51
     summary: ©NJB, Biotechnology Society of Nepal 55 Nepjol.info/index.php/njb Prevalence of Multidrug Resistant Bacteria in Causing Community Acquired Urinary Tract Infection Among the Patients Attending Outpatient Department of Seti Zonal Hospital, Dhangadi, Nepal Tek Raj Awasthi1, Narayan Dutt Pant2*, Puspa Raj Dahal3 1Department of Microbiology, Siddhanath Science Campus, Kanchanpur , Nepal 2Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal 3Department of Microbiology, Trichandra Multiple College, Kathmandu, Nepal Abstract Involvement of multidrug resistant bacteria in causing community acquired infection is very serious public health issue. The main objective of our study was to determine the prevalence of multidrug resistant bacteria in causing community acquired urinary tract infection.
    keywords: aeruginosa; antibiotics; antimicrobial; aureus; bacteria; biotechnology; coli; community; department; different; escherichia; gram; growth; health; hospital; infection; involvement; isolates; kathmandu; mdr; microbiology; multidrug; negative; nepal; patients; positive; prevalence; proteus; region; resistant; samples; shrestha; significant; staphylococcus; study; susceptibility; susceptible; test; total; tract; urinary; urine; uti
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        item: #62 of 115
          id: njb-34
      author: Shrestha, Pritish; Bista, Manisha; Sharma, Prativa; Shrestha, Shristi; Lamichhane, Basanta; Adhikari, Sandeep; Pandey, Binayak Raj; Shrestha, Bhupal Govinda
       title: Phytochemical screening, antimicrobial activity and cytotoxicity of Nepalese medicinal plants Swertia chirayita and Dendrobium amoenum
        date: 2015-12-30
       words: 3196
      flesch: 58
     summary: A, B, C, D, E and F represents ZOI of different plant extract against S. paratyphi, E. coli, K. pneumoniae, S. typhi, P. aerugenosa and S. aureus respectively. Plant extract showed the presence of antioxidants like alkaloid, terpenoids, flavonoids, tannin, glycosides.
    keywords: acetate; activity; amoenum; antimicrobial; biotechnology; brine; chiraito; chirayita; cold; concentration; cytotoxicity; daman; death; dendrobium; dhunche; different; equation; ethyl; extract; extraction; figure; graph; inhibition; journal; lc50; medicinal; medium; methanol; nepal; nepjol.info/index.php/njb; njb; percentage; phytochemical; plants; ppm; research; room; sample; science; screening; shrestha; shrimp; society; solution; species; swertia; temperature; test; value; vol; warm; yield; zoi; zone
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        item: #63 of 115
          id: njb-35
      author: Joshi, Bal K; Louws, Frank J; Yenco, G Craig; Sosinski, Byron R; Arellano, Consuelo; Panthee, Dilip R
       title: Molecular Markers for Septoria Leaf Spot (Septoria lycopersicii Speg.) Resistance in Tomato (Solanum lycopersicum L.)
        date: 2015-12-30
       words: 4755
      flesch: 63
     summary: We studied the inheritance of SLS resistance and identified molecular markers linked to SLS resistance using bulked segregant analysis (BSA) in a segregating F2 population. Subject to verification in independent populations, these markers may be useful for MAS of SLS resistance in tomato.
    keywords: 839; analysis; area; band; biotechnology; blight; breeding; bsa; bulk; carolina; disease; dna; dominant; early; et al; figure; gene; individuals; jhoshi; journal; leaf; lines; lycopersici; markers; mas; molecular; nepal; nepjol.info/index.php/njb; njb; north; parent; pcr; plants; polymorphic; population; primers; rapd; reaction; resistance; score; segregant; septoria; single; size; sls; society; spot; study; susceptible; symptoms; tomato; total; university; vol
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        item: #64 of 115
          id: njb-36
      author: Amgai, Resham Babu; Niroula, Raj Kumar; Pantha, Sumitra; Hamal, Shreya Singh; Tamang, Bishal Gole; Sah, Bindeshwar Prasad; Bhatta, Madan Raj
       title: Marker Assisted Screening of Nepalese rice for bacterial leaf blight (BLB) resistance
        date: 2015-12-30
       words: 1845
      flesch: 61
     summary: BLB resistance rice varieties are highly demanded worldwide. We have detected BLB resistance gene Xa-10 on five accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions.
    keywords: absence; accessions; agriculture; amgai; bacterial; biotechnology; blb; blight; breeding; check; council; different; disease; dna; gene; genetic; journal; landraces; leaf; marker; molecular; nepal; nepalese; nepjol.info/index.php/njb; njb; pcr; plant; presence; research; resistance; rice; screening; society; table; varieties; variety; vol; xa-5
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        item: #65 of 115
          id: njb-37
      author: Thapa, Sandeep; Shrestha, Rajani; Tirewal, Anjali; Sharma, Arjun; K.C., Yuvraj
       title: Isolation of yeast from soil and different food samples and its characterization based on fermentation
        date: 2015-12-30
       words: 3097
      flesch: 54
     summary: The authors are also thankful to Pinnacle Academy, Lalitpur, Nepal and Mr. Sushil Khanal from Central Department of Biotechnology, Trubhuvan University, Nepal for providing us Yeasts samples. Simple staining was performed to elucidate the morphology and arrangement of yeast cells and budding.
    keywords: absorbance; alcoholic; bioethanol; biotechnology; broth; carbon; cells; cerevisiae; characteristics; color; concentration; culture; day; dichromate; different; ethanol; extraction; fermentation; glucose; growth; high; isolated; journal; media; method; microbiol; mucoid; nepal; nepjol.info/index.php/njb; njb; oval; oxidation; peptone; phase; plate; production; purple; sample; smell; smooth; society; solution; solvent; source; spot; standard; study; table; thapa; vol; xylose; yeast
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        item: #66 of 115
          id: njb-38
      author: Maharjan, Mahendra; Madhubala, Rentala
       title: Heat shock protein 70 (HSP70) expression in antimony susceptible/resistant clinical isolates of Leishmania donovani
        date: 2015-12-30
       words: 4441
      flesch: 57
     summary: HSP70 gene copy number, gene expression and HSP70 protein expression was found uniform in both antimony sensitive and resistant clinical isolates. A time dependent increase of HSP70 protein expression in L. donovani field isolates was observed.
    keywords: ag83; analysis; antibody; antimony; biotechnology; blot; cells; clinical; copy; dna; donovani; drug; expression; field; figure; fold; gel; gene; genomic; heat; hindiii; hours; hsp70; hsp70 gene; hsp70 protein; hybridization; induction; infantum; isolates; journal; lane; leishmania; leishmaniasis; level; maharjan; mice; molecular; mutants; nepal; nepjol.info/index.php/njb; njb; northern; number; pcr; pfge; probe; promastigotes; protein; purification; recombinant; resistant; sag; sbiii; sds; sensitive; sequence; shock; single; society; southern; stress; tarentolae; time; vector; vol; western
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        item: #67 of 115
          id: njb-39
      author: Jha, Bimlesh Kumar; Gupta, Birendra Prasad; Maharjan, Prashanna; Kakshapati, Somila; Munankarmi, Nabin Narayan
       title: Effect of lyophilization on infectivity and viral load of Adenovirus
        date: 2015-12-30
       words: 4375
      flesch: 53
     summary: Adenovirus PCR was done in virus infected cell line where a fragment of 161bp was amplified from hexon gene of AdV genome and visualized by 2% agarose gel electrophoresis (Figure 1). To determine the effect of lyophilization on virus titer the lyophilized virus after revival was titrated in Hep-2 cell line and virus titer was determined by TCID50 and PFU following the same procedure as described earlier for the stock virus.
    keywords: adenovirus; adv; biotechnology; buffer; cell; centrifuge; column; culture; difference; dna; drying; effect; figure; freeze; ice; infected; infectivity; insert; jha; journal; ligation; load; lyophilization; lyophilized; methods; min; mini; months; nepal; nepjol.info/index.php/njb; njb; pcr; pellet; rapid; reaction; real; revived; significant; society; sterile; stock; storage; study; supernatant; tcid50; temperature; time; tissue; titer; tube; viral; viral load; virus; viruses; vol; water; white
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        item: #68 of 115
          id: njb-4
      author: Koirala, Niranjan; Khanal, Sareeta; Chaudhary, Sujan; Gautam, Sagar; Sah, Shiv Nandan; Subba, Prince; Marraiki, Najat; Batiha, Gaber El-Saber
       title: Potential surface active agent production using very low grade and cheap substrate by Bacillus subtilis as microbial cell factory
        date: 2021-12-30
       words: 5110
      flesch: 52
     summary: The emulsification activity of cell free broth extracted from soya cake This study recorded the emulsification activity was observed highest in mustard oil with the cell free extract from mustard oil cake whereas engine oil showed the lowest (Figure. 1). Emulsification activity in mustard oil from extract from mustard oil cake was found to be 1.13, 0.99, 0.81, 0.95 and 0.76 at the time interval of 1, 2, 3, 4 and 5 hour respectively.
    keywords: ability; active; activity; applications; applied; bacillus; bacterial; bio; biochemical; biosurfactants; biotechnology; broth; bsn; cake; cell; chemical; cost; crude; day; dec.9; different; e24; emulsification; emulsification index; engine; engine oil; environmental; extract; figure; free; higher; highest; index; industrial; journal; kerosene; koirala; lowest; medium; microbial; microbiology; mustard; mustard cake; mustard oil; nepal; njb; oil; oil cake; photograph; positive; potential; production; properties; range; research; result; soil; soya; soya cake; strain; study; substrate; subtilis; sunflower; sunflower oil; surface; surfactants; table; tension; test; time; university; value; water; whey
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        item: #69 of 115
          id: njb-40
      author: Sah, Surendra Kumar; Khatiwada, Saroj; Chaudhary, Deepak; Jha, Chandra Bhushan; Bhattacharya, Soumya
       title: Doxorubicin induced histomorphometric changes in testes of albino rat
        date: 2015-12-30
       words: 3045
      flesch: 51
     summary: We also observed progressive decrease in testis weight from short term to long term experimental group. As compared to short term experimental group, midterm experimental group and long term experimental group have decreased diameter and cross-sectional area of the seminiferous tubules, which may be due to more effect of the drugs on exposure to long time.
    keywords: albino; animals; area; biotechnology; body; cells; changes; control; cross; damage; diameter; difference; dose; doxorubicin; effects; epithelium; experimental; experimental group; group; health; journal; long; long term; male; midterm; nepal; njb; normal; p<0.001; present; rats; right; sectional; seminiferous; seminiferous tubules; short; short term; significant; single; study; term; term experimental; testicular; testis; testis weight; tubules; volume; week; weight
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        item: #70 of 115
          id: njb-41
      author: Shrestha, Geeta Rajbhandari; Mridha, Amin Udhin
       title: Detection and Quantitation of Aflatoxin for the Diagnosis of Aspergillus flavus
        date: 2015-12-30
       words: 2615
      flesch: 55
     summary: The different strains of A. flavus producing aflatoxins were screened on Aflatoxin Producing Medium (APM) as described by Donald et al. (1981) Aflatoxins production is low during early stages of growth that increases with the decline of growth and the maximum production was recorded after the stationary phase of growth, which soon decline in its production (Table 1 and Figure 1).
    keywords: aflatoxins; aftb1; aspergillus; biotechnology; cancer; carcinoma; cause; contamination; days; detection; different; diseases; effect; elisa; exposure; factors; figure; flavus; food; groopman; growth; health; hepatocellular; highest; human; incubation; level; media; medium; method; mycotoxins; nepal; periods; production; samples; significant; storage; study; sucrose; temperatures; test; tlc
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        item: #71 of 115
          id: njb-42
      author: Upreti, Deepak; Sapkota, Naresh Prasad; Aryal, Bibek; Shakya, Sangita
       title: Accumulation of Poly-hydroxy-butyric Acid (PHB) by Bacillus Strain Isolated from Paddy Field of Kathmandu University Premises
        date: 2015-12-30
       words: 2165
      flesch: 52
     summary: Screening for PHB production To test the production of PHB, seed culture of isolated colonies were grown in 10 ml Nutrient broth and incubated for 24 hours at 30 °C. Results Of the fifteen samples evaluated for PHB production, DNB-6 isolate accumulated the maximum PHB (31.91%) of dry cell weight.
    keywords: accumulation; acid; bacillus; bacteria; biodegradable; biotechnology; cell; chloroform; concentration; curve; deficient; different; dnb-6; figure; glucose; growth; hours; intensity; isolate; maximum; media; nepal; nitrogen; nutrient; phase; phb; poly; polymer; production; soil; strain; time; upreti; yield
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        item: #72 of 115
          id: njb-43
      author: Rosyara, Umesh R; Joshi, Bal K
       title: Association Mapping for Improvement of Quantitative Traits in Plant Breeding Populations
        date: 2022-07-11
       words: 7281
      flesch: 53
     summary: In the present review, we discuss different mapping populations, approaches, prospects and limitations of using association mapping in plant breeding populations. Jan.  2012,  Vol.  2,  No.  1:  72  –  89                                                                                                                    Biotechnology  Society  of  Nepal  (BSN),  All  rights  reserved   72 REVIEW ARTICLE Association  Mapping  for  Improvement  of  Quantitative  Traits  in  Plant  Breeding   Populations   Umesh R. Rosyara1 and Bal K. Joshi2 1  South  Dakota  State  University,  Plant  Science  Department,  Brookings,  South  Dakota,  USA   2  Biotechnology  Unit,  NARC,  Khumaltar,  PO  Box  1135  Kathmandu,  Nepal   *Correspondence  Author  recent  address:  joshibalak@yahoo.com   ABSTRACT DNA-based molecular markers have been extensively utilized for mapping of genes and quantitative trait loci (QTL) of interest based on linkage analysis in mapping populations.
    keywords: 2000; 2004; acad; advanced; allele; analysis; approach; association; association mapping; biotechnology; breeding; bsn; candidate; case; collections; control; core; crop; cross; data; decay; different; disequilibrium; diversity; effects; elite; expected; extended; extreme; families; family; fine; generations; genet; genetic; genome; genomic; genotype; genotyping; germplasm; high; hum; improvement; individuals; information; jan; journal; lines; linkage; loci; low; maize; mapping; markers; method; natl; nepal; number; parents; phenotypic; plant; polymorphism; populations; power; proc; progeny; programs; qtl; quantitative; recent; recombination; related; results; rights; samples; sci; selection; significant; single; size; society; structure; studies; study; synthetic; test; time; trait; transmission; unlinked; usa; use; variation; vol
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        item: #73 of 115
          id: njb-44
      author: Deswal, Sumit
       title: Models of T cell antigen receptor activation: the puzzle still remained
        date: 2022-07-11
       words: 3650
      flesch: 52
     summary: Although the downstream signaling mechanisms of T cell activation have been explored to quite detailed extent, the basic mechanism of how ligand binding to extracellular domains of TCRαβ leads to downstream signaling remained unclear. E-mail: deswal@immunbio.mpg.de, Tel.: +49-761-5108-314, FAX: +49-761-5108-423 Abstract T cell antigen receptor (TCR) is a protein-complex expressed on all T cells of the immune system and is responsible for the activation of T cells when infectious agent is presented by an antigen presenting cell (APC) in the form of peptides bound to the major histocompatibility complex (pMHC).
    keywords: activation; antigen; binding; biotechnology; bsn; cd3; cd4; cd45; cd8; cell; change; class; clustering; complex; conformational; cytoplasmic; geometry; heterodimer; homodimer; immunol; itams; jan; journal; kinase; kinetic; lck; ligand; lipid; membrane; model; molecules; nepal; observations; phosphatase; phosphorylation; pmhc; protein; raft; receptor; rights; sci; segregation; signaling; society; structure; t cell; tcr; vol
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        item: #74 of 115
          id: njb-45
      author: Singh, Dipali; Tripathi, Anushree; Kumar, Gautam
       title: An Overview of Computational Approaches in Structure Based Drug Design
        date: 2022-07-11
       words: 3147
      flesch: 52
     summary: This review summarizes the methods of structure based drug design, usage of related softwares and a case study that explores to find a suitable drug (lead) molecule for the mutated state of H-Ras protein in order to prevent complex formation with Raf protein. Keywords: computer aided drug design, structure based drug design, Ras-protein Introduction Traditionally, new drugs were generated from plants and other natural products through accidental observations and discoveries.
    keywords: acid; active; approaches; binding; biotechnology; bsn; cancer; candidate; cell; compounds; computational; computer; database; dec; design; discovery; docking; drug; drug design; energy; enzyme; field; fit; fragment; functional; grid; growth; hydroxyurea; inhibitors; journal; lead; ligand; methods; molecular; molecule; mutated; nepal; new; novo; p21; potential; process; protein; ras; review; rights; sbdd; scoring; site; small; society; state; structure; target; vol
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        item: #75 of 115
          id: njb-46
      author: Patel, Hardik A; Patel, Rajesh K; Khristi, Sunil M; Parikh, Kruti; Rajendran, Geetha
       title: Isolation and Characterization of Bacterial Endophytes from Lycopersicon esculentum Plant and their Plant Growth Promoting Characteristics
        date: 2022-07-11
       words: 5540
      flesch: 57
     summary: PGPR have been reported to directly enhance plant growth by the production of plant growth regulators, and improvements in plant nutrient uptake [12,16] or indirectly by the production of metabolites like antibiotics, siderophores etc that decrease the growth of phytopathogens [12]. Four of the strains among the 18 showed maximum positive results of plant growth promoting regulators (PGPR) test and among them best probable isolate was selected and thus its 16SrDNA was amplified and sequenced.
    keywords: 16s; acetic; acid; activity; agar; amplified; assay; bacilli; bacteria; biotechnology; bsn; cell; characteristics; chromium; clearance; cocco; culture; different; endophytic; entire; esculentum; flat; gram; growth; growth hr; host; hr10; hr13; hr3; hr4; hr7; hr8; hr9; hrs; iaa; indole; irregular; isolates; jan; journal; lycopersicon; maximum; medium; microbiol; min; morphological; motile; negative; nepal; nutrient; opaque; organic; pectinase; pgpr; phosphate; pigmentation; plant; plant growth; plate; positive; presence; production; promotion; rdna; results; rights; root; rough; round; sequence; siderophore; small; smooth; society; soil; sterile; strains; study; table; tolerance; transparent; vol; yellow; zone
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        item: #76 of 115
          id: njb-47
      author: Latha, Parvatham Madhu; Chanakya, Pallem; Srikanth, Manipati
       title: Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake
        date: 2022-07-11
       words: 2912
      flesch: 48
     summary: [11]. Concentration of Lovastatin (mg/g) = Concentration of Lovastatin (mg/ml) x Dilution factor (DF) Amount of substrate taken (g) Results and Discussion Selection of solid substrates in SSF for lovastatin production: Solid substrates employed in SSF processes are heterogeneous natural raw-materials which are generally insoluble in water and play a dual role-supply of nutrients to the microbial culture growing and anchorage for the growing cells. 2: Effect of moisture content on lovastatin production Effect of Inoculum volume Fermentation was carried out with different inoculum volumes (0.5-4ml) of five day old A. fischeri culture for a period of 144h to study its effect on lovastatin production.
    keywords: aspergillus; biotechnology; bsn; cake; coconut; content; culture; dry; effect; fermentation; fig; fischeri; growth; initial; inoculum; jan; journal; lovastatin; maximum; microbial; moisture; nepal; oil; parameters; present; process; production; rights; society; solid; sources; ssf; state; study; substrate; temperature; time; vol; volume; yield
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        item: #77 of 115
          id: njb-48
      author: Joshi, Bal K; Bimb, Hari P; Kansakar, David; Ghimire, Ekta
       title: Genetic Relationship among Nepalese Rice Landraces and Cultivars based on RAPD Markers
        date: 2022-07-11
       words: 3173
      flesch: 72
     summary: Key words: Genetic distance, rice, RAPD Introduction Nepal is rich in rice genetic resources The three blast resistance genes pyramided rice genotype, A57-115-8 was genetically near with Anpjutte, Tauli and Thapachini.
    keywords: a57; abd; analysis; bands; biotechnology; blast; breeding; bsn; cluster; collection; cultivars; dhan; diversity; dna; genetic; genome; genotypes; hardinath; highest; information; ir-24; jan; jarneli; joshi; journal; kali; khumaltar; lalshar; landrace; line; mansara; markers; min; mugu; nepal; nr10276; nr10414; nrrp; number; plant; polymorphism; pranpyuri; primers; quadrant; rapd; rice; rights; sample; similarity; society; study; table; tag; varieties; vol
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        item: #78 of 115
          id: njb-49
      author: Dulal, Santosh; Ban, Bhupal; Yang, Gi Hyoek; Jung, Hyun Ho
       title: Cloning, Expression, Purification, and Characterization of Clostridium botulinum Neurotoxin Serotype F Domains
        date: 2022-07-11
       words: 5985
      flesch: 54
     summary: Jan.  2012,  Vol.  2,  No.  1:  1  –  15                                                                                                                    Biotechnology  Society  of  Nepal  (BSN),  All  rights  reserved   10 strong affinity towards F-HN and native type F toxin (Figs. 5b and 6b). Conclusion This paper describes molecular and immunological studies on C. botulinum neurotoxin type F domains.
    keywords: affinity; amplified; analysis; antibodies; antibody; binding; biotechnology; bont; botulinum; botulism; bsn; catalytic; cells; chain; chemicals; chromatography; cloning; coli; concentrations; dna; domains; elisa; expression; fig; free; fusion; gst; hcc; heavy; high; immunoreactivity; inc; indirect; induction; iptg; jan; journal; kda; lane; langeland; light; mice; microbiol; min; mouse; native; nepal; neurotoxin; neutralizing; page; pbs; pcr; pgex-4t-1; plasmids; plates; polyclonal; proteins; purification; purified; receptor; recombinant; restriction; rights; ril; sds; sequence; serotype; serum; society; soluble; specific; substrate; subunit; terminal; thrombin; translocation; type; usa; vaccine; vector; vol; western
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        item: #79 of 115
          id: njb-5
      author: Maharjan, Rasna; Thapa, Prakash; Khadayat, Karan; Kalauni, Surya Kant
       title: Phytochemical Analysis and α-Amylase Inhibitory Activity of Young and Mature Leaves of Cinnamomum tamala
        date: 2021-12-30
       words: 5161
      flesch: 58
     summary: C. tamala leaves exhibited significant biological properties with scientific validation including antioxidant [16,17], antimicrobial [18,19,20], anti-diabetic [21,22,23], anticancer [24], and anti-inflammatory Methanolic young leaves extract showed α-amylase inhibition with IC50 value 224.6 ± 2.76 μg/mL as compared to acarbose with IC50 value 5.93 ± 0.14 μg/mL. The result suggests that young leaves of C. tamala had anti-diabetic activity so further work should be carried out.
    keywords: 2):14; absorbance; acarbose; acid; activity; amylase; analysis; antioxidant; available; biotechnol; bsn; c. tamala; carbohydrates; cinnamomum; compounds; concentration; content; control; dec.9; department; diabetes; diabetic; different; diseases; eem; eey; enzyme; extract; extraction; flavonoid; food; gae; health; higher; ic50; inhibition; inhibitory; insulin; internet; leaf; leaves; maharjan; mature; mature leaves; mean; medicinal; mem; methanolic; mey; nepal; nitrophenyl; njb; pancreatic; percentage; phenolic; phytochemical; plants; potential; present; properties; quercetin; research; sample; sci; screening; sem; sep; standard; study; substrate; table; tamala; temperature; tfc; therapeutic; total; tpc; tribhuvan; type; university; value; vitro; wang; yield; young; young leaves
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        item: #80 of 115
          id: njb-50
      author: Dhakal, Dipesh
       title: The Future Science
        date: 2022-07-11
       words: 435
      flesch: 34
     summary: Hence,  the  future  science  should  be  BE  NATURAL   WITH  SUSTAINABILITY!   For  that   the   idea   from   the   developed   community   should   be   mingled   with   the   natural   resources   available   in   hotspots  patches  of  globe,  to  create  a  utopian  era. 
    keywords: current; globe; life; natural; resources; scientific; system
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        item: #81 of 115
          id: njb-51
      author: Dhakal, Dipesh
       title: Race for Excellence
        date: 2022-07-11
       words: 425
      flesch: 48
     summary: Utilizing the science of biology, chemistry, physics, engineering, and information technology to develop products and services of great promise is the threshold of current scientific research. The fundamental backbone of scientific clues and technological intervention relies on course and discourse of the natural attributes within the nature itself; taking into consideration of the physical, chemical, biological and computational prospect.
    keywords: human; implications; life; nature; race; science; scientific; technology
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        item: #82 of 115
          id: njb-52
      author: Adhikari, Raju; Adhikari, Benu
       title: Biotechnology Growth Partnership: A Potential Flagship Program in S&T
        date: 2022-07-11
       words: 2280
      flesch: 44
     summary: Nepal  currently  trades  about  36  different  types  of  products  derived  from  herbal plants and the total revenue earned is reported  to  be  approximately  12  million  NRs  (US  $240,000).  The clinical trials and manufacturing of products could  be  carried  out  in  Nepal  which  will  help  develop  necessary  infrastructure  for  the  production  of  such  value added products and clinical trial facilities  in the  long  run  on  low  cost.   
    keywords: acid; adhikari; areas; australia; biotechnology; cheese; cordyceps; countries; country; government; high; medicinal; mycelia; nepal; overseas; partnership; plants; potential; products; project; raw; research; s&t; scientific; sinensis; value; work; yak
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        item: #83 of 115
          id: njb-53
      author: Ray, Bishnu Pada; Hassan, Lutful; Sarker, Smreeti Kana
       title: In vitro cultivation and regeneration of Solanum melongena (L.) using stem, root and leaf explants
        date: 2022-07-11
       words: 2581
      flesch: 62
     summary: Key words: Regeneration, BAP, NAA.    Correspondence Author:  E‐mail: bpray2003@yahoo.com  Introduction  Brinjal (Solanum melongena L.), belongs to the family  Solanaceae, is one of the most popular, palatable and  nutritious  vegetable  crop  in  Bangladesh.  Abstract  The treatment combinations was BAP (0, 2.0, 3.0 and 4.0 mg/L) and NAA (0, 0.1, 0.5, and 1.0 mg/L).
    keywords: abcde; bap; biotechnology; callus; combinations; days; defg; explants; induction; klmno; leaf; medium; naa; nepal; regeneration; stem
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        item: #84 of 115
          id: njb-54
      author: Ammann, Klaus
       title: Molecular differences between GM- and non-GM crops over-estimated?
        date: 2022-07-11
       words: 10606
      flesch: 29
     summary: Nature,  356, 6364, pp  1‐2     http://dx.doi.org/10.1038/356001b0 AND http:// www.botanischergarten.ch/Regulation/Anonymous‐US‐ Regulation‐Nature‐1992.pdf  Arber, W. (1994)    MOLECULAR EVOLUTION: COMPARISON OF NATURAL  AND ENGINEERED GENETIC VARIATIONS.  Plant Cell Reports, 18,  10, pp  858‐862     http://dx.doi.org/10.1007/s002990050674 AND http:// www.botanischergarten.ch/Mutations/Barnabas‐ Colchicine‐Genome‐Doubling‐1999.pdf  Barros, E. (2010) Molecular Profiling Techniques as Tools to  Detect Potential Unintended Effects in Genetically  Engineered Maize (revised title), Molecular Profiling  Techniques Detect Unintended Effects in Genetically  Engineered Maize (old title).
    keywords: academy; action; america; ammann; analysis; arber; assessment; biosafety; biotechnology; breeding; bsn; case; changes; chromosome; comparison; conclusions; conventional; crops; data; differences; different; dna; dynamics; early; effects; elements; engineering; environmental; evolution; evolutionary; expression; fedoroff; fig; food; gene; genetic; genome; genomic; horizontal; http://; impact; important; isi>://wos; jan; journal; lack; level; lines; locus; maize; mcclintock; methods; molecular; mutation; national; natural; nature; nepal; new; organic; paper; plants; polyploids; possible; proceedings; process; processes; recent; regulation; regulatory; research; rights; risk; sciences; scientific; sequences; similar; society; soltis; species; states; system; time; transcriptome; transfer; transgenesis; transgenic; transposition; united; van; vol; wheat
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        item: #85 of 115
          id: njb-55
      author: Chandan, S; Umesha, S; Bhure, SK; Haraprasad, N; Chandrashekar, S
       title: Development of PCR assay for targeting partial lipL21 and lipL41 gene of leptospira
        date: 2022-07-11
       words: 4502
      flesch: 52
     summary: Primer  degradation  was  suspected  and  the  PCR  reactions  at  the  optimized  conditions  were  repeated with fresh primer set P30/31. PCR reactions  were  again  carried  out  on  nine  serovars  at  MgCl2  Results and Discussion  Specificity  of  Leptospira  spp.  Primers.  The following conditions were chosen: PCR was  performed  in  25  ml  reaction  volume  containing  10x  Taq buffer with KCl (100mM Tris HCl, 50mM KCl, 0.8%  Nonidet P40), 1.5mM MgCl2, 2.5mM of each dNTPs, 2U  of  Taq  polymerase,  25pmoles  of  each  primer  and  0.25ml  of  DNA  (40ng/ml)  extract  from  L.  icterohaemorrhagiae. 
    keywords: agarose; annealing; assay; biotechnology; buffer; detection; dna; enzyme; fig; figure; gel; gene; jan; lane; leptospira; leptospirosis; lipl41; method; min; nepal; p28/29; p30/31; pathogenic; pcr; primer; product; reaction; restriction; run; sequence; serovars; species; specificity; standardized; table; temperature; template
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        item: #86 of 115
          id: njb-56
      author: Shrestha, Raunak; Joshi, Rubin Narayan; Joshi, Kriti; Poudel, Bal Hari; Shrestha, Bhupal Govinda
       title: Analysis of KatG Ser315Thr Mutation in Multidrug Resistant Mycobacterium tuberculosis and SLC11A1 Polymorphism in Multidrug Resistance Tuberculosis in Central Development Region of Nepal Using PCR-RFLP Technique: A Pilot Study
        date: 2022-07-11
       words: 3600
      flesch: 48
     summary: Samples  021,  022,  and  023  has  a  band  size  of  153  bp  indicating  no  mutation  at  315th  codon  position  where  as  sample  020  and  024  has  a  band sized 132 bp indicating SeràThr mutation at 315th  codon position of KatG gene.   This fact remained as a challenge  SampleID  MDR‐TB  INT4 PCR amplicon size  (bp)  ApaI digested product of  INT4 (bp)  G/C Polymorphism in INT4  SLC‐001  POSITIVE  514  514  NEGATIVE  SLC‐002  POSITIVE  514  514  NEGATIVE  SLC‐003  POSITIVE  514  514  NEGATIVE  SLC‐004  POSITIVE  514  514  NEGATIVE  SLC‐005  POSITIVE  514  514  NEGATIVE  SLC‐006  POSITIVE  514  514  NEGATIVE  SLC‐007  POSITIVE  514  514  NEGATIVE  SLC‐008  POSITIVE  514  514  NEGATIVE  SLC‐009  POSITIVE  514  514  NEGATIVE  SLC‐010  POSITIVE  514  514  NEGATIVE  SLC‐011  POSITIVE  514  514  NEGATIVE  SLC‐012  POSITIVE  514  514  NEGATIVE  SLC‐013  POSITIVE  514  514  NEGATIVE  SLC‐014  POSITIVE  514  514  NEGATIVE  SLC‐015  POSITIVE  514  514  NEGATIVE  SLC‐016  POSITIVE  514  514  NEGATIVE  SLC‐017  POSITIVE  514  514  NEGATIVE  SLC‐018  POSITIVE  514  514  NEGATIVE  SLC‐019  POSITIVE  514  514  NEGATIVE  SLC‐020  POSITIVE  514  514  NEGATIVE  SLC‐021  POSITIVE  514  514  NEGATIVE  SLC‐022  POSITIVE  514  514  NEGATIVE  SLC‐023  POSITIVE 
    keywords: agarose; biotechnology; cases; control; dna; drug; figure; fragment; gel; gene; genomic; inh; int4; katg; mdr‐tb; mtb; mutation; negative; nepal; pcr; polymorphisms; positive; region; resistance; restriction; samples; ser315thr; slc11a1; study; tuberculosis
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        item: #87 of 115
          id: njb-57
      author: Mathew, Shiju; Thomas, George; Ahmad, Tufail
       title: An Evaluation of the Fungi Isolated from Sub-epidermal Region of Post-harvested Stored Wheat Grains
        date: 2022-07-11
       words: 2810
      flesch: 59
     summary: Fungal activity can cause undesirable effects in  grains  including  discolouration,  contribute  to  heating  and  losses  in  nutritional  value,  produce  off‐odours,  losses  in  germinability,  deterioration  in  baking  and  milling  quality,  and  can  result  in  contamination  by  mycotoxins (Hocking, 2003; Magan et al., 2003).  Jan. 2011, Vol. 1, No. 1 : 9‐13  13   Biotechnology Society of Nepal (BSN), All rights reserved   Detection  techniques  for  stored‐product  insects  in  grain.
    keywords: agar; allahabad; alternaria; aspergillus; biotechnology; different; epidermis; extract; factors; food; fungal; fungi; grains; harvest; india; losses; microorganisms; mycelium; nepal; quality; samples; solution; species; storage; studies; sub‐epidermal; university; water; wheat
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        item: #88 of 115
          id: njb-58
      author: Joshi, Bal K; Panthee, Dilip R
       title: Information Theory and Multivariate Techniques for Analyzing DNA Sequence Data: An Example from Tomato Genes
        date: 2022-07-11
       words: 4699
      flesch: 63
     summary: Main  objective  was  use  of  information  theory  and  multivariate  techniques  to  understand  diversity  among  genes  and  relate  the  sequence  complexity  with  phenotypes.  Sequences  similarity  among  genes  was  observed within a family.
    keywords: amino; analysis; atchley; biotechnology; cluster; complexity; component; crop; data; disease; diversity; dna; entropy; evolution; fruit; genes; genetic; information; journal; molecular; multivariate; mutual; nepal; potato; principal; protein; pseudomonas; quality; resistance; sequences; species; theory; tomato; value; variation
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        item: #89 of 115
          id: njb-62
      author: Daniel, Imaobong E.; Akpan, Ekemini I.; Utam, Edidiong C.
       title: Phytochemical Evaluation, Antioxidant and Antimicrobial Activities of Various Extracts from Leaves and Stems of Bryophyllum pinnatum
        date: 2020-07-31
       words: 8255
      flesch: 56
     summary: Magnesium strip and HCl were mixed with plant extracts. Inhibition of Listeria monocytogenes and Aeromonas hydrophila by plant extracts in refrigerated cooked beef.
    keywords: ability; absorbance; acetate; acetate extracts; acetate leaf; acetate stem; acid; activities; activity; aeruginosa; agar; albican; alkaloids; anthocyanins; antimicrobial; antimicrobial activity; antioxidant; antioxidant activity; assay; aureus; bacteria; bioactive; biotechnol; bryophyllum; bryophyllum pinnatum; bsn; candida; capacity; chemistry; coli; color; compounds; concentrated; concentration; crude; daniel; determination; determined; different; different extracts; distilled; dpph; escherichia; ethyl; ethyl acetate; extracts; ferric; figure; flavonoids; food; frap; free; highest; human; ic50; inhibition; isolates; jan; journal; leaf; leaf extracts; leaves; medicinal; methanol; methanol ethyl; methanol extracts; methanol leaf; methanol stem; method; mixture; ml methanol; nepal; niger; nigeria; njb; organisms; parts; phenolic; phytochemical; pinnatum; plant; plant extracts; polyphenols; power; presence; present; present present; properties; radical; reagent; research; resistant; results; salmonella; saponins; scavenging; screening; solution; spp; standard; stem; stem extracts; study; table; tannins; test; total; vitro; water
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        item: #90 of 115
          id: njb-64
      author: Joshi, Bal Krishna; Joshi, Darbin; Ghimire, Surya Kanta
       title: Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers
        date: 2020-07-31
       words: 5501
      flesch: 64
     summary: Knowledge on genetic diversity is important to manage and use of finger millet landraces and genotypes properly. We have used finger millet landraces from just few farmers of two districts.
    keywords: accessions; agricultural; amplification; analysis; applied; assessment; bands; biotechnol; breeding; bsn; buffer; cluster; conservation; coracana; correlation; crop; data; dendrogram; development; dhading; different; distance; diversity; dna; eleusine; et al; figure; finger; finger millet; gaertn; gel; genetic; genetic diversity; genotypes; germplasm; group; highest; information; joshi; joshi et; journal; jun; kalo; kaski; landraces; lowest; major; markers; mean; millet; millet landraces; min; molecular; nei; nepal; nepal j; njb; number; pic; plant; polymorphic; population; primers; principal; profiles; purbeli; rapd; rapd markers; research; resources; scoring; ssr; ssr markers; ssr-06; study; table; ugep-10; ugep-53; upgma; value
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        item: #91 of 115
          id: njb-66
      author: Bhandari, Ganesh; Pokhrel, Basant; Oli, Yogesh; Katuwal, Archana; Bhandari, Netra Lal
       title: Screening of Methicillin Resistant Staphylococcus aureus (MRSA) from Wounds in Pediatric Patients Visiting Tertiary Care in Hospital
        date: 2019-12-29
       words: 4020
      flesch: 55
     summary: 278 Sensitive % Resistant % Amikacin Ciprofloxacin Cotrimoxazole Cefoxitin Erythromycin Cloxacillin Cefotaxime Penicillin Ofloxacin Chloramphenicol Gentamicin 236 100 125 176 166 180 236 28 116 255 264 84.89 35.97 44.96 63.30 59.7 64.74 84.89 10.08 41.72 91.72 94.96 42 178 153 102 112 98 42 250 162 23 14 15.10 64.02 55.04 36.69 40.28 35.25 15.10 89.92 58.27 8.27 5.03 Table 7. Resistant % Sensitive %
    keywords: agar; age; antibiotic; aureus; bacterial; bhandari; biotechnology; cefoxitin; children; chloramphenicol; clinical; culture; department; different; distribution; female; figure; gender; gentamicin; growth; health; hospital; infections; isolates; journal; laboratory; male; methicillin; microbiology; mrsa; mssa; nepal; nepjol.info/index.php/njb; njb; number; outpatients; patients; pattern; penicillin; positive; prevalence; pus; resistant; s. aureus; samples; sensitivity; society; staphylococcus; staphylococcus aureus; study; susceptibility; table; total; university; vol
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        item: #92 of 115
          id: njb-67
      author: Kumari, Rinki
       title: Potential of Ayurvedic Drugs in Differentiating Neuronal Stem Cells from Human Breast Milk: A Review
        date: 2019-12-29
       words: 6480
      flesch: 50
     summary: Recently, Stem cell therapy has revolutionized excellent clinical therapy with the potential of stem cells to differentiate into various cell types and it may help to replace different cell lines of an organism. Stem cells, able to construct every type of human tissues or organ and due to unlimited self-renewal to produce progeny exactly the same as the originating cell with the extremely regulated mode.
    keywords: 2015; able; acid; active; activity; adult; alternative; alzheimer; angelica; applicable; application; ayurveda; ayurvedic; biotechnology; blood; body; bone; brain; breast; breast milk; cell therapy; cells; chinese; clinical; complement; components; development; differentiation; disease; disorders; drugs; effect; embryonic; et al; evidence; expression; extract; factor; family; figure; formulation; ginkgo; good; growth; hbm; health; herbal; high; hmscs; human; human breast; important; indian; induced; infants; int; issues; journal; kim; kumari; like; lineage; literature; mammary; marker; marrow; mechanisms; medhya; medicinal; medicine; mesenchymal; milk; molecules; nepal; nepjol.info/index.php/njb; nestin; neural; neurodegenerative; neurogenesis; neurogenic; neuronal; neurons; njb; non; organ; papaya; placenta; plant; polyherbal; positive; potential; presence; process; progenitor; proliferation; properties; rasayana; regenerative; repair; research; review; role; root; scientific; sinensis; single; society; source; specific; stem; stem cells; stimulant; studies; study; system; therapeutic; therapy; tissue; traditional; treatment; type; use; vol; zhang
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        item: #93 of 115
          id: njb-68
      author: Thapa, Rojina; Thapa, Rambika; Mahat, Sunita; Thapa, Rupak
       title: Polyphenols: Nature's Gift as dietary phytoconstituents against different human ailments
        date: 2019-12-29
       words: 5310
      flesch: 54
     summary: Yamada H, Watanabe H: Tea polyphenols in preventing cardiovascular diseases. Yamada H, Watanabe H: Tea polyphenols in preventing cardiovascular diseases.
    keywords: 2012; acids; action; activity; age; aging; anticancer; antidiabetic; antioxidant; bioavailability; biol; biotechnology; blood; bond; cancer; cancerous; cardiovascular; cardiovascular diseases; catechin; cell; chem; coffee; components; compounds; constituents; consumption; daily; death; derivatives; diabetes; dietary; different; diseases; effects; factors; figure; flavonoids; food; free; fruits; glucose; grapes; green; health; high; http://dx.doi.org/; human; important; intake; intestine; journal; lignans; low; mechanism; med; metab; natural; nepal; nepjol.info/index.php/njb; neurodegenerative; nutr; oxidative; phenolic; phenyl; plant; polyphenols; potential; prevention; process; products; promising; properties; quercetin; radicals; range; red; research; resveratrol; rich; risk; role; scavenging; society; sources; stilbene; stress; studies; study; synthesis; tea; thapa; vegetables; vol; wide; wine
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        item: #94 of 115
          id: njb-69
      author: Maharjan, Elina; Shakya, Pooja; Bhattachan, Balkrishna; Baral, Bharat Prasad; Shrestha, Dhiraj
       title: Phenotypic characterization of beta-lactamases producing Gram-negative bacteria in a tertiary hospital, Nepal
        date: 2019-12-29
       words: 4319
      flesch: 61
     summary: [17] and 4% Nepal et al in 2017 Poudyal S, Bhatta DR, Shakya G, Upadhyaya B, Dumre SP, Buda GC, et al: Extended- Spectrum beta-lactamase producing multidrug resistant clinical bacterial isolates at National Public Health Laboratory, Nepal.
    keywords: abr; acinetobacter; aeruginosa; antibiotic; antimicrobial; bacteria; beta; biotechnology; care; cefpodoxime; center; clinical; coli; control; data; disc; effective; esbl; extended; gram; health; higher; hospital; imipenem; isolates; journal; klebsiella; laboratory; lactamase; maharjan; management; mbl; mdr; metallo; method; microbiol; negative; nepal; nepjol.info/index.php/njb; njb; pneumoniae; positive; producers; production; resistance; samples; society; spectrum; spp; strains; study; table; test; total; vol; zoi
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        item: #95 of 115
          id: njb-70
      author: Kumari, Rinki; Ilango, K.; Singh, G.P.I.; Dubey, G.P.
       title: Pharmacological In vivo test to evaluate the antidepressant activity of polyherbal formulation
        date: 2019-12-29
       words: 6282
      flesch: 52
     summary: The results indicated that polyherbal formulation exhibited significant antidepressant activity, as indicated by its ability to decrease force swim stress, induced immobility time in rats as well as restoring the biogenic amines to normal level that were altered by the swim induced stress in whole rat brain. The present study can be summarized by following finding: 1. the force swim stress procedure caused depressive-like behavior in treatment rats, as observed by different assays such as mirror chamber test and elevated plus maze test; 2. significant reduction in depressive- like behaviors was evident in the stressed rats treated with PF and Sertraline (positive control); 3. forced swim test procedures imbalance the neurotransmitter and induced inflammatory injury along with oxidative damage by increasing lipid peroxidation in the treated rats and 4. PF and Sertraline treatments exerted protective effects against forced swim stress and increase the level of neurotransmitter and decrease the glutathione in the rat brain.
    keywords: 14th; 28th; 7th; acetylcholine; acid; activities; activity; animals; antidepressant; antioxidant; anxiety; arbortristis; behavioral; biochemical; biol; biotechnology; body; brain; chronic; cognitive; content; control; damage; data; days; decrease; depression; different; disorders; drug; effect; entries; extract; figure; forced; formulation; fst; glutathione; group; hippophae; immobility; increase; journal; kumari; level; life; like; lipid; major; mao; melatonin; mice; min; model; monoamine; nepal; nepjol.info/index.php/njb; neurotransmitter; njb; noradrenalin; number; nyctanthes; ocimum; open; oxidase; oxidative; peroxidation; pharmacol; plants; polyherbal; positive; potential; present; rats; reduced; reduction; results; reuptake; role; salcifolia; selective; serotonin; sertraline; significant; society; somnifera; standard; stressed; studies; study; swim; system; test; time; treatment; university; values; vol; water; withania
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        item: #96 of 115
          id: njb-71
      author: Chhetri, Bijay
       title: Nematode Fauna Associated with Kiwi (Actinidia delicosa, Chev.) Plants in Machchhegaun, Kathmandu, Nepal
        date: 2019-12-29
       words: 3321
      flesch: 60
     summary: No any published data about study of plant nematodes was found from study area. So, these four order of plant nematodes have been reported for the first time associated with Kiwi plants from Machchhegaun in Nepal.
    keywords: anterior; area; biotechnology; central; chhetri; crop; discolaimus; distribution; dorylaimus spp; eucephalobus spp; figure; free; helicotylenchus; helicotylenchus spp; iotonchus spp; journal; kathmandu; kiwi; kiwi plants; living; machchhegaun; magnification; mesorhabditis spp; mononchus; nematodes; nepal; nepjol.info/index.php/njb; njb; number; order; organic; parahadronchus; parahadronchus spp; parasitic; plants; posterior; predatory; present; production; region; rhizosphere; samples; society; soil; species; spp; study; total; tylenchida; vol
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        item: #97 of 115
          id: njb-74
      author: Pandey, Binayak Raj; Shrestha, Angela; Sharma, Nisha; Shrestha, Bhupal Govinda
       title: Evaluation of Phytochemical, Antimicrobial, Antioxidant Activity and Cytotoxic Potentials of Agave americana
        date: 2019-12-29
       words: 4482
      flesch: 54
     summary: Figure 1 Graphical comparison of antimicrobial activity of A. americana extract against different clinical isolates. The graph shows antioxidant assay of A. americana extract in comparison to ascorbic acid as the standard.
    keywords: activities; activity; agave; agave americana; americana; antibiotics; antimicrobial; antioxidant; assay; aureus; bacillus; biotechnology; breast; calculated; cancer; cell; clinical; concentration; control; cytotoxic; cytotoxicity; death; different; dmso; dpph; ethnomedicinal; extract; extraction; figure; flavonoids; ic50; journal; line; medicinal; medicine; method; negative; nepal; nepjol.info/index.php/njb; njb; non; pandey; phytochemical; plant; presence; properties; radical; research; resistant; results; saponins; scavenging; screening; shrestha; society; standard; study; table; thuringiensis; value; vol
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        item: #98 of 115
          id: njb-75
      author: Shakya, Shubha Ratna; Labh, Shyam Narayan
       title: Ethanol Extract of Choerospondias axillaris Fruit Pulp Enhances Haematological Parameters in Oncorhynchus mykiss Cultured in Nepal
        date: 2019-12-29
       words: 5833
      flesch: 60
     summary: In intensive aquaculture stress level in fish impairing immune responses against pathogens leads to disease outbreaks in fishes Discussion In fishes, blood is a patho-physiological reflector of the entire body and the haematological parameters such as leucocyte count, erythrocyte count, hematocrit and hemoglobin are routinely tested to monitor the health status of fish and any deviation from the normal ranges may indicate a disturbance in the physiological process.
    keywords: 2003; agents; analysis; antibiotics; aquaculture; axillaris; biol; biotechnology; blood; cage; cells; changes; chemical; choerospondias; cold; compounds; concentration; content; control; count; diet; dietary; different; disease; drugs; effective; effects; environment; experimental; extract; factors; fed; feed; fish; fishes; fruit; group; growth; haematocrit; haematological; haematological parameters; haematology; haemoglobin; hct; health; herbal; herbs; higher; immune; immunity; increase; india; ingredients; journal; kg-1; labh; lapsi; maximum; mchc; mcv; mean; medicinal; minimum; mykiss; natural; nepal; nepjol.info/index.php/njb; nile; njb; non; oil; oncorhynchus; parameters; phyllanthus; physiological; plants; present; production; pulp; rainbow; rbc; res; resistance; response; sci; shakya; shellfish; significant; similar; society; specific; status; stimulants; stress; study; system; table; tilapia; total; trout; use; value; vitamin; vol; water; white
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        item: #99 of 115
          id: njb-76
      author: Shrestha, Dhiraj; Thapa, Pratigya; Bhandari, Dinesh; Bhattachan, Balkrishna; Parajuli, Hiramani; Chaudary, Prakash; Sharma, Vijay Kumar; Shah, Pradeep Kumar
       title: Detection of Pyuria by Microscopic Urinalysis as a Marker of Pediatric Urinary Tract Infection
        date: 2019-12-29
       words: 3234
      flesch: 56
     summary: We confirmed 64 (18.1%) UTI cases by culture, the gold standard for UTI diagnosis. Fever was the most common clinical manifestation in UTI cases.
    keywords: biotechnology; cases; children; clinical; common; culture; data; diagnosis; febrile; female; fever; health; healthcare; infants; infection; journal; kathmandu; laboratory; male; marker; microbiology; microscopic; microscopic urinalysis; negative; nepal; nepjol.info/index.php/njb; njb; patients; pediatric; positive; predictive; pyuria; samples; sensitivity; society; specificity; study; symptoms; table; test; total; tract; treatment; urinalysis; urinary; urinary tract; urine; uti; value; vol
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        item: #100 of 115
          id: njb-77
      author: Agrahari, Gaurav; Koirala, Amrit; Thapa, Roshan; Chaudhary, Mahesh Kumar; Tuladhar, Reshma
       title: Antimicrobial Resistance Patterns and Plasmid Profiles of Methicillin Resistant Staphylococcus aureus Isolated from Clinical Samples
        date: 2019-12-29
       words: 4008
      flesch: 49
     summary: Terry Alli OA, Akinloye O, Rowley DA, Butcher PD: A comparative assessment of ribosomal DNA polymorphisms in methicillin resistant Staphylococcus aureus (MRSA) epidemiology. ORIGINAL RESEARCH ARTICLE Antimicrobial Resistance Patterns and Plasmid Profiles of Methicillin Resistant Staphylococcus aureus Isolated from Clinical Samples Gaurav Agrahari1, Amrit Koirala1, Roshan Thapa1, Mahesh Kumar Chaudhary2 and Reshma Tuladhar1* 1Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal, 2KIST Medical College, Imadol, Kathmandu, Nepal, Abstract Methicillin-resistant Staphylococcus aureus (MRSA), showing resistance to several antibiotics is a global health problem associated with considerable mortality and morbidity.
    keywords: agarose; agrahari; alkaline; analysis; antibiotic; antimicrobial; aureus; biotechnology; care; cefoxitin; clinical; coagulase; college; community; department; detection; different; diffusion; disc; dna; epidemiology; figure; gel; hospital; imadol; isolated; journal; kathmandu; lysis; meca; medical; methicillin; method; microbiology; molecular; mrsa; nepal; nepjol.info/index.php/njb; njb; patients; pattern; penicillin; plasmid; positive; prevalence; profile; resistant; resistant staphylococcus; s. aureus; samples; sensitivity; society; specimens; standard; staphylococcus; staphylococcus aureus; strains; studies; study; susceptibility; techniques; test; vancomycin; vol
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        item: #101 of 115
          id: njb-82
      author: Manzum, Amika Ahmed; Mamun, Md Arafat Al
       title: Isolation of Bacillus spp. Bacteria from Soil for Production of Cellulase
        date: 2019-01-15
       words: 2790
      flesch: 54
     summary: [8].Therefore in this study an investigation was undertaken to isolate Bacillus bacteria capable of producing cellulase enzyme. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples.
    keywords: activity; api; bacillus; bacteria; biotechnology; cellulase; cellulolytic; culture; doi; enzyme; food; glucose; industrial; isolates; isolation; journal; kit; large; mamun; manzum; media; medium; microbiol; min; nepal; nepjol.info/index.php/njb; njb; positive; production; research; results; samples; society; soil; species; spp; strains; study; test; vol
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        item: #102 of 115
          id: njb-83
      author: Baniya, Amina; Singh, Sushma; Singh, Minu; Nepal, Pragya; Adhikari, Mahesh; Aryal, Sagar; Adhikari, Anurag
       title: Isolation and Screening of Antibiotics Producing Streptomyces spp from the Soil Collected around the Root of Alnus nepalensis from Godawari
        date: 2019-01-15
       words: 4649
      flesch: 51
     summary: NASA 108(ZOI-29.66mm) and NASA 113(ZOI-26.4mm) showed antibacterial activity against Imepenem resistant E. faecalis (Figure 2). NASA 103, NASA 104, NASA 105, NASA 106, NASA 107, NASA 109, NASA 110, NASA 111, NASA 114, NASA 115, NASA 201, NASA 301, NASA 302, NASA 305, Nepal Journal of Biotechnology.
    keywords: + +; actinomycetes; activity; agar; alder; alnus; antibacterial; antibiotics; antimicrobial; baniya; biotechnology; characterization; coli; culture; dark; different; diversity; extraction; fermentation; figure; genome; gray; growth; imepenem; inhibition; isolates; isolation; journal; klebsiella; lalitpur; mdr; mdrs; media; methicillin; method; microbial; microorganisms; mrsa; multidrug; mycelium; nasa; nepal; nepalensis; nepjol.info/index.php/njb; njb; non; number; pathogens; pneumoniae; primary; research; resistant; rhizosphere; screening; secondary; slimey; society; soil; species; starch; strains; streptomyces; substrate; table; treatment; tree; vancomycin; vol; vre; white; whitish; yellow +; zoi; zone
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        item: #103 of 115
          id: njb-84
      author: Kumar, Ashok; Sharma, Palvi; Mahajan, Anupama; Begum, Zarina
       title: In Vitro Comparative Study of Antioxidant and Antibacterial Activity of Selected Dietary Plants
        date: 2019-01-15
       words: 3729
      flesch: 48
     summary: Due to drug resistance of pathogens, efforts have been made to find their substitute for treatment of diseases and with knowledge of antimicrobial activity of plant extracts, they may play a significant role in cure of microbial diseases [16]. 10mg/ml of Ascorbic acid used as reference for compare results of plant extracts.
    keywords: ability; activities; activity; aeuroginosa; agar; alkaloids; aloe; analysis; antibacterial; antimicrobial; antimicrobial activity; antioxidant; antioxidant activity; ascorbic; auerus; bacillus; biotechnology; bud; coli; diffusion; effect; escherichia; extract; flavonoids; flower; food; free; garlic; ginger; glycosides; growth; herbal; inhibition; journal; kumar; maximum; medicinal; method; mtcc; nepal; nepjol.info/index.php/njb; njb; phytochemical; plants; pseudomonas; radical; research; result; scavenging; sci; science; screening; singh; society; species; spectrophotometric; standard; staphylococcus; study; subtilis; table; terpenoids; test; turmeric; vol
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        item: #104 of 115
          id: njb-85
      author: Shah, Gyanendra Bikram; Gurung, Krishna; Baral, Bharat Prasad; Gurung, Ram Raja; Bhattachan, Balkrishna
       title: Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors
        date: 2019-01-15
       words: 3578
      flesch: 63
     summary: ©NJB, Biotechnology Society of Nepal 33 Nepjol.info/index.php/njb Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors Gyanendra Bikram Shah1, 2, Krishna Gurung1, Bharat Prasad Baral1, Ram Raja Gurung3, Blood donors in Pokhara Valley were screened for anti-HCV antibodies, anti-HBV antibodies using third generation ELISA kits and automated ELISA Processor in serology laboratory at Central Blood Transfusion Service (CBTS) of Nepal Red Cross Society (NRCS) in Pokhara, Nepal.
    keywords: age; biotechnology; blood; blood donors; cancer; chronic; cirrhosis; criteria; cross; data; diseases; distribution; donation; donors; elisa; factors; female; gender; group; hbv; hcv; health; hepatitis; higher; highest; infection; infection rate; journal; liver; method; nepal; nepjol.info/index.php/njb; njb; old; patients; people; pokhara; rapid; rate; risk; seroprevalence; shah; significant; society; study; table; tattoo; test; total; transfusion; virus; vol; volunteer; wise; year
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        item: #105 of 115
          id: njb-86
      author: Baral, Bharat Prasad; Koirala Sharma, Binita; Gurung, Krishna; Gurung, Ram Raja; Supram, Hosuru Subramanava; Bhattachan, Balkrishna
       title: Fecal carriage of Extended Spectrum β-Lactamases (ESBL) Producing Escherichia coli and Klebsiella spp. among School Children in Pokhara, Nepal
        date: 2019-01-15
       words: 3561
      flesch: 56
     summary: Conversely, the findings of another study [20] showed the variable result with E. coli isolates, 33(9%) were ESBL producers. This study aimed to determine the prevalence of ESBL producing E. coli and Klebsiella spp.
    keywords: antibiotics; antimicrobial; bacteria; baral; biotechnology; care; cefotaxime; ceftazidime; children; clinical; coli; collected; control; data; e. coli; esbl; escherichia; extended; figure; gram; hospital; infections; isolates; journal; klebsiella; klebsiella spp; laboratory; lactamase; mdr; nepal; nepjol.info/index.php/njb; njb; organisms; patients; pneumoniae; pokhara; positive; prevalence; producer; resistance; samples; school; society; spectrum; spp; stool; study; susceptibility; test; total; vol
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        item: #106 of 115
          id: njb-87
      author: Aryal, Sajesan; Hada, Aroj; Hamal, Sanjay; Prajapati, Abhishek; Timilsina, Paras Mani; Adhikari, Sandeep
       title: Development of Cost Optimized Horizontal Gel Electrophoresis Running Unit for Developing Countries (Nepal)
        date: 2019-01-15
       words: 2958
      flesch: 61
     summary: *Corresponding Author Email: acsandeep2014@gmail.com Introduction In graduate courses and research environment, the use of gel electrophoresis seems to be an expensive technique. This protocol upon optimization can be effectively embraced by the high schools, colleges and even universities to demonstrate the basic principle of gel electrophoresis and provide each student with an equal opportunity to get acquainted with the physical as well as working mechanisms of the gel electrophoresis equipment at a low cost.
    keywords: agarose; aluminum; anode; bands; biotechnology; box; buffer; cathode; cost; countries; dna; electrodes; electrophoresis; expensive; figure; fragments; gel; hindiii; image; laboratory; ladder; lambda; migration; neb; nepal; nichrome; plastic; platinum; run; safe; samples; separation; standard; students; tray; unit; usd; use; wires
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        item: #107 of 115
          id: njb-91
      author: Shakya, Shubha Ratna
       title: Effect of Herbs and Herbal Products Feed Supplements on Growth in Fishes: A Review
        date: 2017-12-31
       words: 4198
      flesch: 60
     summary: An artificial feed used in the aquaculture improves fish growth with maximum weight in short time [3]. New substances are added in fish feed to improve feed conversion efficiency that result in fish growth [4].
    keywords: activity; additives; allium; antibiotics; aquaculture; better; biotechnology; body; carp; carpio; catla; common; composition; consumption; control; conversion; cyprinus; diet; dietary; different; digestive; diseases; effect; efficiency; egypt; fed; feed; fenugreek; fingerlings; fish; fish growth; fishes; garlic; growth; growth performance; health; herbal; herbs; immunity; improved; increase; indian; journal; labeo; major; maximum; medicinal; mossambicus; nepal; nepjol.info/index.php/njb; nile; niloticus; njb; oreochromis; oreochromis niloticus; performance; plants; powder; production; products; protein; quality; rate; red; response; rohita; sativum; sci; sgr; shakya; society; stress; supplementation; supplemented; survival; tilapia; university; use; utilization; vitamin; vol; weight; world
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        item: #108 of 115
          id: njb-92
      author: Giri, Gorakh Raj; Timilsina, Uddhav
       title: Apoptosis: Implications in Viral and Mycobacterium tuberculosis infections
        date: 2017-12-31
       words: 7305
      flesch: 50
     summary: Both viral and Mycobacterium tuberculosis (Mtb) infections modulate host cell apoptosis for their benefits [6,9–11]. Thus, evidence suggests that host cell apoptosis is crucial for host resistance to Mtb infection.
    keywords: 1997; acad; activation; active; adenovirus; alveolar; anti; antiapoptotic; apoptosis; apoptotic; bad; bak; baltim; barr; bax; bcl2; bh3; bid; binding; biol; biotechnology; blocks; caspase; cell; cell apoptosis; cell death; chem; chen; complex; cytochrome; cytomegalovirus; death; degradation; dependent; dna; dnase; domains; epstein; et al; executioner; expression; extrinsic; factor; family; fas; fasl; figure; flip; formation; function; genes; giri; granzyme; hiv-1; host; human; immune; immunol; induction; infected; infection; inhibition; inhibits; initiator; interaction; intracellular; intrinsic; journal; large; leading; lee; ligand; like; macrophages; mechanisms; members; membrane; microbiol; mitochondrial; modulation; mol; molecular; mtb; mycobacterium; mycobacterium tuberculosis; natl; necrosis; nepal; nepjol.info/index.php/njb; new; njb; p53; pathological; pathway; permeability; physiol; proapoptotic; proc; protein; receptor; regulation; release; response; role; sci; signals; society; stimuli; survival; tat; timilsina; tnf; tnfα; trail; transmembrane; trigger; tuberculosis; tumor; upregulates; viral; virulent; virus; viruses; vol; wang; xiap
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        item: #109 of 115
          id: njb-93
      author: Rimal, Ujjwal; Thapa, Shovana; Maharjan, Roshani
       title: Prevalence of Extended Spectrum Beta-Lactamase Producing Escherichia coli and Klebsiella species from Urinary Specimens of Children attending Friendship International Children’s Hospital
        date: 2017-12-31
       words: 4741
      flesch: 55
     summary: In our study, 25.5% of E. coli isolates and 12.5% of Klebsiella pneumoniae isolates were ESBL producer. Regular and routine monitoring of ESBL producing isolates is essential.
    keywords: amoxicillin; antibiotic; antimicrobial; bacterial; beta; biotechnology; ceftazidime; ceftriaxone; children; ciprofloxacin; clinical; coli; cotrimoxazole; drug; e. coli; enzymes; esbl; escherichia; escherichia coli; extended; female; growth; hospital; imipenem; infections; international; isolates; journal; kathmandu; klebsiella; klebsiella pneumoniae; laboratory; lactamase; nepal; nepjol.info/index.php/njb; nitrofurantoin; njb; oxytoca; patients; pattern; pneumoniae; positive; prevalence; producers; rate; resistance; result; rimal; samples; sensitive; significant; similar; society; spectrum; spp; strains; studies; study; susceptibility; table; test; total; tract; urinary; urine; vol
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        item: #110 of 115
          id: njb-94
      author: Chaudhary, Pradip Kumar; Shrestha, Mitesh; Poudel, Bal Hari; Adhikari, Mahesh Kumar
       title: In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
        date: 2017-12-31
       words: 2639
      flesch: 59
     summary: ©NJB, Biotechnology Society of Nepal 28 Nepjol.info/index.php/njb induce development of fruiting bodies from wild edible mushroom V. bombycina. A variety of waste materials have been used for cultivation of Volvariella mushroom that include paddy straw, water hyacinth, oil palm bunch and oil palm pericarp waste, banana waste, sawdust, cotton waste, sugar cane bagasse, composted mixtures of tropical wood wastes and pineapple skin waste.
    keywords: 25oc; bags; biotechnology; bodies; bombycina; chaudhary; collected; cultivation; culture; days; development; edible; environmental; factors; figure; formation; fruiting; growth; incubation; journal; medicinal; mushroom; mycelium; nepal; nepjol.info/index.php/njb; njb; paddy; pink; plant; populus; primordial; pure; silky; society; spawn; species; stage; straw; substrates; temperature; tree; university; volvacea; volvariella; volvariella bombycina; waste; weeks; white; wild
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        item: #111 of 115
          id: njb-95
      author: Subedi, Munal; Magar, Mamata Gharti; Rajbhandari, Gita Shrestha
       title: Assessment of Quality of Underground Drinking Water: Very near (≤20 meters) and Far (>50 meters) from the River
        date: 2017-12-31
       words: 3887
      flesch: 59
     summary: 300 ml of underground water samples were collected from 100 different households located near site (≤20 meters) and far site (>50 meters) from Bagmati river. However, in areas ≤10 meter distance, pH ranged from 7.3-8.9, whereas in water samples from areas >50 meters distance pH ranged from 6.9-7.9.
    keywords: ammonia; analysis; areas; bagmati; biological; biotechnology; chloride; coliform; conductivity; contamination; content; different; distance; drinking; far; fecal; figure; health; high; higher; households; journal; kathmandu; meters; meters distance; method; near; nearer; nepal; nepjol.info/index.php/njb; nitrate; njb; parameters; physiochemical; polluted; pollution; purpose; quality; range; river; samples; sites; society; sources; standard; study; subedi; treatment; underground; underground water; valley; values; vol; water; ≤10; ≤20
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        item: #112 of 115
          id: njb-96
      author: Thapa, Kabi; Bhattachan, Balkrishna; Gurung, Raja Ram; Katuwal, Archana; Khadka, Jagat Bahadur
       title: Antibiotic Resistance Pattern of Shigella spp. Among Gastroenteritis Patients at Tertiary Care Hospital in Pokhara, Nepal
        date: 2017-12-31
       words: 4007
      flesch: 57
     summary: ©NJB, Biotechnology Society of Nepal 14 Nepjol.info/index.php/njb Antibiotic Resistance Pattern of Shigella spp. The main aim of our study is to isolate and identify Shigella spp.
    keywords: acid; age; ampicillin; antibiotic; antimicrobial; bacterial; biotechnology; boydii; cases; children; ciprofloxacin; clinical; collected; countries; dis; disease; drugs; dysenteriae; flexneri; gastroenteritis; gram; group; hospital; india; infection; isolated; journal; khan; laboratory; med; method; nalidixic; negative; nepal; nepjol.info/index.php/njb; njb; old; patients; pattern; positive; prevalence; rate; regional; resistance; s. sonnei; sample; shigella; shigella spp; shigellosis; society; sonnei; species; spp; stool; study; test; thapa; total; trimoxazole; vol; western; years
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        item: #113 of 115
          id: njb-97
      author: Thusa, Rojeena; Mulmi, Sushika
       title: Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata
        date: 2017-12-31
       words: 4907
      flesch: 59
     summary: Test for Reducing Sugar To 0.5ml of plant extract, 1ml of water and 5-8 drops of Fehling’s solution was added and heated over water bath. Test for Cardiac Glycosides 5ml of plant extract was treated with 2ml of glacial acetic acid containing one drop of FeCl3 solution.
    keywords: + +; acetate; acid; activity; antimicrobial; aristata; aureus; authentic; berberine; berberis; biotechnology; brine; cm-1; color; compound; dark; department; different; ethyl; extract; figure; green; hexane; isolated; isolation; journal; leaf; mahonia; mahonia nepalensis; methanol; mn2; mn3; mulmi; nepalensis; nepjol.info/index.php/njb; njb; orange; phytochemical; plant; precipitate; presence; r.f; red; resistant; sample; screening; shrimp; society; solution; spectrum; spot; staphylococcus; stem; sticky; stretch; table; test; thusa; tlc; value; vol; yellow; yield
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        item: #114 of 115
          id: njb-98
      author: Devkota, Hari Prasad; Miyazaki, Sena; Yahara, Shoji
       title: Amentoflavone and Kaempferol Glycosides from the Aerial Parts of Cissampelos pareira
        date: 2017-12-31
       words: 1870
      flesch: 66
     summary: Keywords: Cissampelos pareira; Menispermaceae; Batulpate; Flavonoids; Amentoflavone *Corresponding Author Email: devkotah@kumamoto-u.ac.jp Introduction Cissampelos pareira L. (Family: Menispermaceae), commonly known as “Batulpate” in Nepal, is a climbing herb with perennial root-stock. Borthakur M, Devi N, Mahanta R: Antifertility activity of the methanolic leaf extract of Cissampelos pareira in female albino mice.
    keywords: aerial; alkaloids; amentoflavone; biotechnology; chemical; cissampelos; compounds; constituents; extract; family; flavonoids; gel; glucopyranoside; glucuronopyranoside; glycosides; isolated; kaempferol; menispermaceae; meoh; nepal; nmr; o--d; pareira; parts; plant; silica; table; water
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        item: #115 of 115
          id: njb-99
      author: Bastakoti, Srijana; Belbase, Shiva; Manandhar, Shrinkhala; Arjyal, Charu
       title: Trichoderma species as Biocontrol Agent against Soil Borne Fungal Pathogens
        date: 2017-12-31
       words: 4403
      flesch: 49
     summary: Keywords: Biocontrol, Trichoderma species, inhibition percentage, soil borne pathogens and dual culture technique *Correspondence Author Email: srijanabastakoti33@gmail.com Introduction Soil borne plant pathogenic fungi cause heavy crop losses all over the world. Biological control of plant diseases, especially those caused by soil borne plant pathogens and nematodes, by microorganisms has been considered a more natural and environmentally acceptable alternative to the existing chemical treatment methods [3].
    keywords: action; agent; agricultural; antibiotics; bastakoti; biocontrol; biological; biotechnology; borne; chemical; collected; colony; culture; days; diameter; different; disease; dual; effective; efficacy; environment; enzymes; figure; fungal; fungal pathogens; fungi; fusarium; growth; incubation; inhibition; isolates; isolation; journal; nepal; nepjol.info/index.php/njb; njb; pathogens; pathology; pda; percentage; plant; plate; potential; presence; radial; region; research; rhizoctonia; rolfsii; samples; sclerotionia; sclerotium; sclerotium rolfsii; society; soil; solani; species; study; technique; test; trichoderma; trichoderma species; vol
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