Bibliography
This is an automatically generated bibliography describing the content of this study carrel.
- njb-1
- author: Baral, Adesh; Gorkhali, Ritesh; Basnet, Amit; Koirala, Shubham; Bhattarai, Hitesh K
- title: Efficacy and Toxicity of Different Forms of Asparaginases Against Acute Lymphoblastic Leukemia: A Review
- date: 2021-12-30
- words: 6954
- flesch: 56
- summary: In a comparison done between intermittent and continuous routes of PEG E. coli asparaginase among 625 children of Europe, it was found that 95% of patients were asparagine depleted during treatment There were also specific toxicities seen in the group given PEG E. coli asparaginase.
- keywords: activity; acute; anti; arm; asparaginase; asparaginase activity; asparaginase group; average; baral; biotechnol; blood; bsn; cancer; cells; children; chrysanthemi; chrysanthemi asparaginase; clinical; coli; coli asparaginase; concentration; der; different; dose; e. coli; efficacy; enzyme; erwinia; escherichia; escherichia coli; et al; event; forms; free; grade; group; higher; hypersensitivity; induction; intramuscular; leukemia; lymphoblastic; native; native e.; native escherichia; nepal; njb; overall; pancreatitis; patients; peg; peg asparaginase; peg e.; peg escherichia; period; phase; range; reaction; recombinant; sluis; studies; study; survival; table; therapeutic; therapy; toxicity; treatment; trial; type; van; weeks; years
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- njb-106
- author: Thapa, Aishwarya; Budhathoki, Anupa; Sapkota, Anupama; Sainju, Muskan; Shrestha, Prativa; Prasad Pant, Shyam
- title: Isolation, Identification and Screening of Bacillus species with Antimicrobial Activity from Different Soil Samples of Kathmandu Valley
- date: 2021-12-30
- words: 3695
- flesch: 50
- summary: Zone of inhibition demonstrated by antimicrobial metabolite producing isolates against test organisms Sample Isolate Number Name of the isolate S. aureus E. coli Pseudomonas spp. Hence, this study determines the soil in Kathmandu Valley as a potential source of antimicrobial producing Bacillus spp.
- keywords: activity; agar; antibiotic; antimicrobial; aureus; bacillus; bacillus spp; bacteria; biotechnol; bsn; characterization; coli; colonies; compounds; crude; different; esbl; extract; gram; growth; inhibition; inhibitory; isolates; isolation; kathmandu; method; motile; mrsa; negative; nepal; njb; nutrient; organisms; positive; primary; production; pseudomonas; resistance; salmonella; samples; screening; soil; species; spore; spp; study; test; thapa; valley; zone
- versions: original; plain text
- njb-108
- author: Joshi, Bal Krishna; Ghimire, Krishna Hari; Bista, Prakash Raj; Yadaw, Ram Baran; Shrestha, Ram Krishna; Kharel, Gaurish Krishna; Paneru, Prakash; KC, Ram Bahadur; Bhandari, Deepak
- title: Intellectual Property Right on Basmati Rice: Current Scenario and Evidences of Origin, Diversity, Cultivation and Use Values of Basmati Rice in Nepal
- date: 2021-07-31
- words: 10753
- flesch: 63
- summary: Because of its high market value at global level, many countries and organizations have been attempting to get intellectual property rights (mainly patent and geographical indication tag) on Basmati rice. India applied for GI tag to Basmati rice in the European Union (EU) in July 2018, and Nepal submitted opposition letter along with proofs and evidences of origin, diversity, cultivation and use values of Basmati rice on 9 December 2020.
- keywords: 1981/08/31; 1994/09/22; 600; acharya; agricultural; agrobiodiversity; agronomy; ancient; areas; aromatic landraces; aromatic rice; ason; available; bajhang; bajura; bara; basmati; basmati dhan; basmati jhapa; basmati rice; basmati type; basmati::irgc; bhandari; bharatpur; bhatta; biodiversity; biotechnol; bird; bsn; center; chaudhary; chitwan; community; conservation; countries; crop; cultivation; dadeldhura; dec; dhan; dhan::irgc; different; districts; diversity; dna; doti; editors; et al; evidences; farm; farmers; figure; fine; gauchan; genebank; genetic; geographical; ghaiya; ghimire; gorkha; grain; group; hansaraj; high; historical; india; indication; information; international; internet; ipgri; irgc; jhapa; jhinuwa; joshi; joshi bk; joshi et; kailali; kalanamak; kalo; kanchanpur; kaski; kathmandu; khanal; khumaltar; krishna; lamjung; landraces; local; long; markers; market; masino; medium; morang; municipality; narc; national; native; nepal; nepal basmati; nepal j; nepalese; ngrc; njb; nuniya; origin; parsa; paudel; plant; pokhara; products; proofs; quality; ramechhap; rato; registered; research; resources; rice; rice grain; rice landraces; rice varieties; sali; science; seed; seto; shivasatasi; short; shrestha; singh; siraha; society; special; sthapit; subedi; sunaulo; sunsari; table; tag; tarai; technology; time; total; traditional; traits; tulsi; type; type rice; use; values; varieties; word
- versions: original; plain text
- njb-109
- author: Kunwar, Saroj; Khatiwada, Saroj; Gelal, Basanta; Thapa, Saroj; Shah, Gaurishankar; Baral, Nirmal; Lamsal, Madhab
- title: Thyroid Function and Thyroglobulin Level in Iodine-Deficient Children of Eastern Nepal
- date: 2021-07-31
- words: 3060
- flesch: 61
- summary: Several studies, particularly in eastern Nepal show promising results in the improvement of iodine deficiency as revealed by an increase in median urinary iodine concentration (UIC) of school children In another study in eastern regions, Dhankuta and Dharan, iodine deficiency was 26.6% and 15.6% respectively among school children [13, 14].
- keywords: age; blood; children; concentration; data; deficiency; district; doi; eastern; free; ft3; ft4; function; gelal; health; hormones; hypothyroidism; insufficient; intake; iodine; lamsal; low; median; nepal; normal; nutrition; participants; range; reference; samples; school; serum; status; studies; study; subclinical; thyroglobulin; thyroid; tsh; uic; urinary
- versions: original; plain text
- njb-112
- author: Pakuwal, Evance; Manandhar, Prakash
- title: Comparative Study of Nutritional Profile of Rice Varieties in Nepal
- date: 2021-07-31
- words: 5024
- flesch: 64
- summary: The results also highlight the cooking and physicochemical properties of rice depending on the amylose content of rice varieties. Among those, pigmented rice varieties such as red, black, purple, and brown rice are known to be consumed for a long time in Southeast Asian countries.
- keywords: activity; amylose; analysis; anthraquinone; antioxidant; available; bath; biotechnol; black; black rice; bsn; characteristics; content; cooking; determination; different; different rice; extract; food; glycoside; grains; highest; journal; jumli; length; manandhar; marsi; marsi rice; method; minutes; mixed; moisture; nepal; njb; nutritional; pakuwal; phenolic; photograph; phytochemical; pigmented; presence; present; properties; protein; red; respectively; results; rice; rice varieties; rice variety; sample; saponin; solution; standard; study; sugar; table; tannin; time; varieties; variety; water
- versions: original; plain text
- njb-113
- author: Shrestha, Dhiraj; Bhattachan, Balkrishna; Parajuli, Hiramani; Shrestha, Sujata
- title: Avian/Bird flu: A review: H5N1 outbreaks in Nepal
- date: 2021-07-31
- words: 14271
- flesch: 57
- summary: Guan Y, Peiris JSM, Lipatov AS, Ellis TM, Dyrting KC, Krauss S, Zhang LJ, Webster RG, Shortridge KF: Emergence of multiple genotypes of H5N1 avian influenza viruses in Hong Kong SAR. Duan L, Bahl J, Smith GJD, Wang J, Vijaykrishna D, Zhang LJ, Zhang JX, Li KS, Fan XH, Cheung CL: The development and genetic diversity of H5N1 influenza virus in China, 1996–2006. Virology 2008, 380(2):243-254.
- keywords: acid; acute; agriculture; aiv; aivs; animal; antibodies; antibody; antigenic; antiviral; ards; areas; asia; avian influenza; biotechnol; birds; blood; bsn; care; case; cell; chan; change; chen; chickens; china; clade; clinical; close; collected; commercial; contact; contaminated; control; countries; current; days; dead; death; definition; detection; diagnosis; different; direct; disease; domestic; dose; drift; drug; ducks; e.g.; east; effective; engl; epidemiology; et al; evidence; evolution; exposure; feb; figure; genes; genetic; global; guan; guidelines; h1n1; h5n1; h5n1 avian; h5n1 infection; h5n1 influenza; h5n1 virus; h7n9; hand; health; health organization; hemagglutinin; high; hong; host; hpai; human; human h5n1; human infection; human transmission; illness; immune; infected; infection; influenza; influenza virus; international; isolation; j u; journal; jsm; kathmandu; kawaoka; kong; laboratory; lancet; layers; lee; like; lim; live; lower; major; management; mar; measures; meat; med; medical; microbiology; migratory; molecular; mortality; multiple; mutations; national; nature; nepal; nepal j; neuraminidase; new; njb; non; nphl; oie; onset; organization; oseltamivir; outbreak; pandemic; pandemic influenza; pathogenic; pathogenic avian; pathogenicity; patients; pcr; peiris; person; pneumonia; polymerase; population; possible; potential; poultry; preparedness; prevalence; preventive; probable; products; proper; protein; public; rapid; rate; recent; replication; research; respiratory; response; results; review; risk; role; samples; serum; severe; shrestha; sites; situation; smith; south; species; specific; specimen; spread; start; strains; studies; study; subtypes; surveillance; swab; symptoms; syndrome; table; test; therapy; threat; time; tract; transmission; treatment; update; vaccines; variation; veterinary; vietnam; viral; virology; virus; virus infection; viruses; vtm; water; webster; weeks; wild; workers; world; world health; years; zhang
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- njb-114
- author: Shrestha, Tara; Lamichhane, Janardan
- title: Assessment of phytochemicals, antimicrobial, antioxidant and cytotoxicity activity of methanolic extract of Tinospora cordifolia (Gurjo)
- date: 2021-07-31
- words: 3281
- flesch: 50
- summary: It is suggested that T. cordifolia methanol extract when taken orally has increased the erythrocytes membrane lipid peroxide and catalase activity [11] T. cordifolia is a well-known medicinal plant in traditional medicine, and several recent scientific investigations have highlighted its potential value in modern medicine. Brine shrimp lethality assay The toxic property of T. cordifolia methanolic extract was determined using the brine shrimp lethality
- keywords: 100mg; 25mg; 50mg; acid; activity; alkaloids; analysis; antibacterial; antimicrobial; antioxidant; ascorbic; bioassay; biotechnol; brine; bsn; concentration; cordifolia; cytotoxicity; disc; diseases; dmso; dpph; extract; extraction; figure; free; glycosides; inhibition; journal; jul; lamichhane; lc50; lethality; medicinal; methanol; nepal; njb; pharmaceutical; phytochemicals; plant; presence; radical; research; review; sample; scavenging; shrestha; shrimp; standard; stem; streptococcus; test; tinospora; traditional; triterpenes; zone
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- njb-115
- author: Maharjan, Bidhya; Karki, Shovana Thapa; Maharjan, Roshani
- title: Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children's Hospital
- date: 2021-07-31
- words: 7514
- flesch: 57
- summary: 57(98.28%) Doxycycline 0(0%) 2(3.44%) 56(96.56%) S. aureus isolates with complete resistance to Vancomycin (MIC≥16µg/ml) are termed as Vancomycin resistant S. aureus (VRSA).
- keywords: 2017; age; antibiotic; antimicrobial; association; aureus; azithromycin; bacteriological; biotechnol; bsn; care; cases; children; chloramphenicol; ciprofloxacin; clindamycin; clinical; coli; college; constitutive; correlation; culture; department; different; diffusion; disc; distribution; doi; doxycycline; drug; erythromycin; et al; figure; friendship; gram; group; growth; high; hospital; icu; increase; india; inducible; infection; international; isolates; journal; kathmandu; linezolid; maharjan; mcg; mdr; medical; meropenem; methicillin; microbiology; mrsa; mssa; multidrug; negative; nepal; njb; opd; organism; pant; patients; pattern; positive; prevalence; profile; pus; research; resistant; resistant staphylococcus; result; s. aureus; samples; sci; sensitive; shrestha; similar; spp; staphylococcus; staphylococcus aureus; strain; study; surgical; susceptibility; swab; table; tertiary; test; total; vancomycin; ward; wound; years
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- njb-116
- author: Adhikari, Bibek; Shah, Pradeep Kumar; Karki, Roman
- title: Antibiogram and Phytochemical Analysis of Cinnamon, Clove, and Sichuan Pepper Extracts
- date: 2021-07-31
- words: 5655
- flesch: 56
- summary: Several infectious diseases are treated using a wide range of plant extracts since they possess antimicrobial potentials. Alkaloids, flavonoids, phenolic compounds, and tannins are the essential phytochemicals present in the plants with possible therapeutic activities and these phytochemicals obtained in plant extracts have demonstrated antimicrobial potential against a wide range of infectious microorganisms
- keywords: absolute; acid; activities; activity; adhikari; aeruginosa; agar; albicans; analysis; antibacterial; antifungal; antimicrobial; antioxidant; aromaticum; aureus; biotechnol; bsn; candida; cinnamomum; cinnamon; clove; coli; concentration; content; different; distilled; effect; effective; essential; et al; ethanolic; eugenol; extracts; flavonoids; folin; food; gallic; gentamicin; higher; highest; india; inhibition; journal; mean; medicinal; methanol; method; microorganisms; mirabilis; nepal; njb; oil; pepper; pharm; phytochemicals; plant; polyphenol; potential; prepared; properties; property; proteus; pseudomonas; reagent; research; responsible; result; salmonella; sci; sharma; species; spices; standard; staphylococcus; study; syzygium; table; tannins; test; typhi; water; world; zanthoxylum; zoi; zone
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- njb-117
- author: K.C., Mamata; Lamichhane, Anuj
- title: Advances in Agricultural Biotechnology
- date: 2021-07-31
- words: 6648
- flesch: 50
- summary: Tissue culture has been used extensively to create genetic variability through the in-vitro culture of protoplasts, anthers, microspores, ovules, and embryos, to improve crop plants and to increase the number of desirable germplasm available to the plant breeder. Disease resistance genes are also transferred using somatic hybridization like asymmetric somatic hybridization was used to transfer bacterial blight resistance trait from wild Oryza meyeriana L. to Oryza sativa L. ssp.
- keywords: abiotic; acid; adoption; advances; agriculture; analysis; applications; available; bacterial; banana; biotechnology; blight; breeding; bsn; cells; certain; commercialized; concerns; conditions; content; cotton; countries; crops; cultivation; development; different; discovery; disease; dna; economic; effects; embryo; engineering; environmental; events; farmers; food; gene; genetic; genome; genomics; golden; haploid; health; herbicide; higher; human; hybridization; improvement; increase; insect; internet; jul; jun; lamichhane; line; maize; major; marker; material; molecular; nepal; new; njb; novel; number; omics; organisms; oryza; papaya; pest; plant; population; potato; potential; production; productivity; protein; quality; recent; rescue; resistance; review; revolution; rice; risks; sativa; sci; seeds; selection; somatic; soybean; species; specific; status; stress; structure; techniques; technologies; time; tissue; tolerance; tolerant; tomato; tools; traditional; traits; transfer; transgenesis; transgenic; transgenic crops; use; varieties; virus; wheat; wide; world; yield
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- njb-119
- author: Budhathoki, Shailesh; Shrestha, Anima
- title: Screening of Actinomycetes from Soil for Antibacterial Activity
- date: 2020-12-30
- words: 5935
- flesch: 48
- summary: Actinomycetes have broad spectrum antibacterial activity Among test organisms, Staphylococcus aureus was found to be the most susceptible with susceptibility toward 12 actinomycetes isolates followed by Klebsiella pneumoniae, susceptible towards 10 isolates of actinomycetes. Bar diagram showing susceptibility of test organisms towards actinomycetes isolates.
- keywords: -110; actinomycetes; actinomycetes isolates; active; activity; aeruginosa; agar; agents; agri; alkaline; antibacterial; antibiotic; antimicrobial; aureus; bacillus; biotechnol; brown; bsn; budhathoki; characterization; coli; colony; damp; days; dec; different; discovery; effective; findings; forest; fractions; gram; growth; india; inhibitory; isolates; isolation; journal; klebsiella; laboratory; land; metabolites; microbiology; moisture; mrsa; mssa; nepal; neutral; njb; novel; number; organisms; paratyphi; plates; pneumoniae; positive; potential; primary; production; range; research; resistant; salmonella; samples; screening; secondary; shrestha; soil; staphylococcus; staphylococcus aureus; strains; streptomyces; study; subtilis; susceptible; table; temperature; test; test organisms
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- njb-120
- author: Koirala, Barsha; Shrestha, Angela
- title: Comparative Study of Bioactive Compounds in Different Varieties of Pears in Nepal
- date: 2020-12-30
- words: 5011
- flesch: 62
- summary: Qualitative tests for sugars/carbohydrates in pear juice The various tests for sugars (Molisch’s test, Benedict’s test, Barfoed’s test, Seliwanoff test, Fehling’s test, Bial’s test, and Iodine test), protein test and phytochemicals (Catechins, Flavonoids, Cyclic glycosides, Terpenoids) were performed for pear juices. They were present in high amount in Chojuro, Kosui and Pharping pears.
- keywords: + +; absorbance; acid; acidity; analysis; anthocyanins; antioxidants; ash; barfoed; bartlette; benedict; bial; biotechnol; catechins; chinese; chojuro; clarity; color; concentration; content; cyclic; dec; denote; different; dye; flavonoids; food; fructose; fruits; glucose; glycosides; highest; iodine; juice; koirala; kosui; local; mixed; moisture; nepal; parameters; pears; pharping; phenolic; physicochemical; phytochemicals; positive; positive test; presence; red; research; sample; shrestha; solution; study; sucrose; sugars; table; tannins; terpenoids; test; total; value; varieties; vitamin; water; weight; yakumo
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- njb-129
- author: Pathak, Enish; Sanjyal, Arjun; Regmi, Chhatra Raj; Paudel, Saroj; Shrestha, Anima
- title: Screening of Potential Plant Growth Promoting Properties of Bacillus Species Isolated from Different Regions of Nepal
- date: 2021-07-31
- words: 3912
- flesch: 58
- summary: This study was aimed to isolate Bacillus species from soil of different regions of Nepal and screen for their ability to promote plant growth directly or indirectly by testing their ability to produce plant growth hormone indole acetic acid, hydrogen cyanide, ammonia and protease as well as phosphate solubilization. The isolated strains showed positive results to maximum PGPR traits and exhibited a potential to be used as alternatives to chemical fertilizers and pesticides and could be used as low-cost bio-based technology to promote plant growth in the agricultural sector.
- keywords: + +; ability; acid; agar; ammonia; bacillus; biotechnol; brown; chemical; colonies; color; cyanide; diameter; different; fertilizers; figure; filter; growth; halo; hcn; iaa; incubation; isolates; kathmandu; laboratory; light; medium; microbiol; nepal; njb; paper; pathak; pgpr; phosphate; pink; plant; plant growth; positive; production; properties; protease; reagent; rhizobacteria; samples; soil; solubilization; species; spp; strains; study; table; test; traits; width; yellow; zone
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- njb-132
- author: Gunasinghe, Y.H.K.I.S.; Rathnayake, I.V.N.; Deeyamulla, M.P.
- title: Plant and Plant Associated Microflora: Potential Bioremediation Option of Indoor Air Pollutants
- date: 2021-07-31
- words: 8828
- flesch: 46
- summary: Removal ratio of gaseous toluene and xylene transported from air to root zone via the stem by indoor plants. Can hydroculture be used to enhance the performance of indoor plants for the removal of air pollutants?
- keywords: activities; aerial; air; air pollutants; air pollution; air quality; area; aromatic; asparagus; associated; atmos; aureum; available; bacteria; benzene; biol; biological; biotechnol; bsn; building; cancer; capability; cause; chemical; cleavage; commutatum; compounds; concentration; conditions; contaminants; davallia; degradation; degrading; deremensis; dracaena; effects; efficiency; elsevier; endophytes; environ; environmental; epipremnum; et al; exposure; factors; fejeensis; ficus; filtration; fittonia; foliage; formaldehyde; formaldehyde removal; gaseous; growth; gunasinghe; health; hedera; helix; high; higher; hours; humans; hydrocarbons; h–1; illnesses; impact; increase; indoor; indoor air; indoor plants; initial; inorganic; internet; japonica; kays; kim; leaf; level; literature; liu; ltd; matter; mechanisms; methods; microbial; microdasys; microflora; microorganisms; mmol; m–3; m−2; nepal; nitrogen; njb; nose; nox; organic; ornamental; outdoor; particles; particulate; parts; philodendron; phyllosphere; phytoremediation; plant; plant species; podophyllum; pollutants; polluted; pollution; poor; potential; potted; ppm; problems; pseudomonas; purification; quality; range; reduction; ref; remediation; removal; res; research; review; role; root; safe; sansevieria; schefflera; sci; short; soil; sources; spathiphyllum; species; strategies; stress; studies; substances; systems; table; temperature; term; thiravetyan; throat; time; toluene; total; toxic; trifasciata; uptake; use; van; ventilation; voc; vocs; volatile; wallisii; xylene; zamiifolia; zamioculcas; zone
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- njb-133
- author: Shresta, Shrimita; Bhattarai, Bibek Raj; Adhikari, Bikash; Rayamajhee, Binod; Poudel, Pramod; Khanal, Santosh; Marasini, Bishnu P.; Aryal, Babita; Bhandari, Sudip; Parajuli, Niranjan
- title: Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants
- date: 2021-07-31
- words: 7666
- flesch: 49
- summary: Hence, the antibacterial activity of plant extracts reported here would be beneficial to identify some potent secondary metabolites as future drug candidates for the therapeutic measures of MDR-strains-induced infections in Nepal and beyond. To obtain plant extracts, the filtrates were concentrated in a rotary evaporator at 50 °C.
- keywords: 767; acacia; acid; activities; activity; adenophorum; aeruginosa; antibacterial; antibacterial activity; antimicrobial; antioxidant; ardens; atcc; aureus; australis; bioactive; biotechnol; biotechnology; bsn; catechu; chrysanthemum; clinical; coli; compounds; control; cordifolium; cumini; diabetes; dmso; drug; eclipta; effects; esculenta; eupoterium; evaluation; extracts; food; free; gae; guajava; highest; hypericum; ic50; indica; indicium; journal; kathmandu; leaves; lee; m. s.; mangifera; marsupium; mdr; medicinal; medicinal plants; microbiology; morus; myrica; neomycin; nepal; njb; officinale; pathogens; phytochemical; plant; plant extracts; pneumoniae; properties; prostrata; psidium; pterocarpus; quercetin; resistant; robusta; scavenging; shorea; shrestha; smallanthus; sonchifolius; species; standard; strains; study; syzygium; table; tfc; tpc; treatment; urtica; value; xanthomonas; zingiber; zoi
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- njb-16
- author: Poudel, Santosh; Paudyal, Akash; Sharma, Bishnu Prasad; Sharma, Kamana; Baral, Yubaraj; Adhikari, Shailaja; Shakya Hada, Manju Shree
- title: Microbial and Physico-Chemical Quality Assessment of Rivers of Kathmandu Valley
- date: 2021-12-30
- words: 5118
- flesch: 63
- summary: The main objective of the study is to detect the physio-chemical and microbiological parameters of water sample from the Bagmati river and its tributaries of Kathmandu valley along with antibiotic susceptibility. Physical Parameters of water samples.
- keywords: alkalinity; ammonia; analysis; antibiotics; assessment; available; bagmati; bagmati river; balkhu; biotechnol; bishnumati; bod; bsn; chemical; chloride; coli; coliform; dec; dhobi; dissolved; guidelines; hanumante; higher; highest; human; i.e.; isolates; journal; kathmandu; level; lowest; method; microbiological; nepal; njb; oxygen; parameters; phosphate; physico; pollution; poudel; previous; quality; r r; r s; resistance; river; s s; sample; study; sundarijal; susceptibility; t u; temperature; total; treatment; tributaries; tukucha; turbidity; u r; valley; value; wastewater; water
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- njb-161
- author: Amgai, Resham B.; Pokharel, Shreejan; Pantha, Sumitra; Parajuli, Atit; Subedi, Sudeep; Dhital, Shambhu P.
- title: Selection of Pyramided Barley Advanced Lines for Stripe Rust, Leaf Rust and Crown Rust Diseases Using Molecular Markers
- date: 2020-12-30
- words: 2892
- flesch: 63
- summary: Therefore, one polymorphic sequence tagged sites (STS) marker (ABG054) for stripe rust resistance, two polymorphic simple sequence repeats (SSR) markers (Bmac0144h and HVM049) for leaf rust and one polymorphic SSR marker (Bmag0006) for crown rust resistance were used to select the advanced barley lines (at F8 stage) from above parents. Selection, identification and incorporation of rust resistance genes is the only option for the development of rust resistance barley varieties for Nepal.
- keywords: 115; abg054; acc#2478; advanced; barley; biotechnol; bold; bonus; breeding; bsn; crosses; crown; dec; disease; field; figure; gene; hordei; hvm049; leaf; lines; major; marker; mas; microsatellite; min; molecular; nepal; nepalese; njb; note; parents; pcr; polymorphism; pyramided; pyramiding; research; resistance; rust; selection; solu; ssr; stage; stripe; table; uwa; variety
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- njb-170
- author: Pawar, Rasika; Zambare, Vasudeo; Nabar, Bela
- title: Comparative Assessment of Antibiotic Resistance in Lactic Acid Bacteria Isolated from Healthy Human Adult and Infant Feces
- date: 2020-10-22
- words: 4458
- flesch: 48
- summary: Antibiotic resistance was exhibited by lactic acid bacteria against most commonly used antibiotics and it was higher in strains isolated from adult fecal samples than in the strains isolated from infant fecal samples. The increasing trend in antibiotic resistance from infant to adult might be due to food habits and antibiotic intakes.
- keywords: acid; acid bacteria; adult; agar; ampicillin; analysis; animal; antibiotic; antibiotic resistance; antimicrobial; assessment; bacteria; biotechnol; bsn; cefotaxime; cefuroxime; chloramphenicol; ciprofloxacin; clinical; colonies; colonization; cultures; data; dec; development; enterococcus; erythromycin; feb; fecal; feces; food; gentamycin; gut; health; higher; human; india; infant; infant fecal; isolated; issue; journal; lab; lactic; lactic acid; lactobacillus; levels; microbiology; microflora; nepal; njb; pawar; pediococcus; penicillin; plates; present; probiotics; resistance; safety; samples; sensitive; software; special; spp; strains; study; susceptibility; tobramycin; vancomycin; whonet; world
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- njb-171
- author: Bhalshakar, Chhaya
- title: Study of the Impact of Organic Manures and Biofertilizers on growth of Phaseolus aureus Roxb.
- date: 2020-10-22
- words: 4011
- flesch: 66
- summary: The biological manure helps to increase crop yields, and also plays a vital role in the nutrient accessibility in soil by improving the physical, chemical, and biological structure of soil, and it enhances the utilization of applied fertilizers Weed plants compete with the agricultural crops; they cause a tremendous reduction in crop yields and increase their production costs.
- keywords: achyranthes; agriculture; analyses; atcb; atvb; aureus; azotobacter; bacteria; bhalshankar; biod; biofertilizer; cake; chemical; con; control; crop; dose; dry; efficiency; fertilizers; fresh; growth; ha-1; higher; highest; increase; india; journal; kg ha-1; lowest; manures; mixed; ncb; neem; neemcake; nepal; nitrogen; npk; organic; phosphate; plant; production; ratio; roxb; significant; single; soil; table; tephrosia; total; treatment; vermicompost; weed; weight; yield
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- njb-174
- author: Suwal, Meena Maiya; Lamichhane, Janardan; Gauchan, Dhurva Prasad
- title: Regeneration Technique of Bamboo Species through Nodal Segments: A Review
- date: 2020-07-31
- words: 11693
- flesch: 65
- summary: The effect of coconut water on bamboo shoot multiplication has been reported by different researchers. Future research must be focused to generate a large scale of bamboo plants.
- keywords: 3mg; acid; ads; arya; ascorbic; asper; axillary; b. balcooa; balcooa; bamboo; bamboo species; bambusa; bap; better; biology; biotechnology; break; browning; bsn; bud; cell; coconut; collection; combination; concentration; condition; contamination; coumarin; culm; culture; dendrocalamus; development; different; edible; effective; embryogenesis; establishment; et al; explants; factors; field; forest; giganteus; growth; hamiltonii; hardening; high; hormones; iaa; iba; important; induction; influence; initiation; jan; journal; jul; kalia; l naa; l y; large; liquid; liquid ms+; mass; mass multiplication; mature; media; medium; method; mg/ l; micropropagation; mother; ms media; ms+ bap; ms+ iba; ms+ naa; multiplication; munro; naa; nees; negi; nepal; njb; nodal; nodal explants; nutans; organ; period; plantlets; position; problem; production; proliferation; protocols; ramanayake; rapid; rate; regeneration; regulators; researchers; review; rooting; roxb; saxena; scale; science; season; segments; sharma; shoot; shoot multiplication; singh; size; soil; solid; somatic; sood; species; strength; strictus; sucrose; supplement; surface; suwal; tdz; technique; tissue; treatment; tulda; u l; vitro; vulgaris; wall; water; world; years; ½ ms+
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- njb-175
- author: Kumari, Rinki; Dubey, G.P.
- title: Standardization of Ayurvedic Drug- Nyctanthes arbor-tristis, Hippophae salicifolia, Ocimum tenuiflorum and Reinwardtia indica and Combined Herb-Herb
- date: 2020-07-31
- words: 10500
- flesch: 62
- summary: In India, Ayurvedic Pharmacopeia is not progressively sufficient to guarantee the quality and virtue of plant extracts’ use as a herbal drug. R. indica extract is widely used by local communities for different purposes such as for tongue wash, to bring about an increase in - lactation period, a remedy against skin diseases, as an anti-infection agent etc.
- keywords: + +; absent; academy; acetic; acharya; acid; agar; agnivesha; alcohol; analysis; arbor; area; ash; ayurvedic; biotechnol; black; black powder; blue; brown; brown brown; brown green; brown powder; bsn; carbohydrate; cells; charaka; chowkhamba; clinical; collected; color; combined; compounds; content; dark; dark brown; different; dridhabala; drug; dubey; editors; epidermis; estimation; ethanol; ether; evaluation; extract; extractive; figure; flavonoids; fluorescence; formulation; fruit; green; green black; green green; green powder; greenish; hcl; health; herb; herbal; hippophae; hptlc; hrs; hydroalcoholic; indica; inorganic; insoluble; j u; journal; krishnadas; kumari; leaf; light; light brown; light yellow; like; material; matter; medicinal; medicine; method; n. arbor; naoh; nepal; nepal j; njb; nmt; parameters; parenchyma; peak; percentage; petiole; phytochemical; plant; polyherbal; powder; presence; present; proximate; quality; r. indica; results; salicifolia; samhita; sample; section; single; soluble; solution; standard; standardization; sthana; study; sulphated; sulphuric; sutra; system; table; tenuiflorum; test; total; track; traditional; transverse; treatment; trichomes; tristis; value; vascular; vyt; water; wavelength; weight; yellow; yellow powder; yellowish; yellowish brown
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- njb-176
- author: Mohammed, Messaoudi; Maroua, Merah
- title: Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits
- date: 2020-07-31
- words: 4546
- flesch: 59
- summary: Tests of antioxidant capacity The antioxidant capacity of the four varieties were evaluated using free radical scavenging test (DPPH) and total antioxidant capacity (TAC). Total antioxidant capacity of fig flowers fruits methanolic extracts.
- keywords: absorbance; acid; activity; analysis; anthocyanins; antioxidant; antioxidant capacity; ascorbic; bakor; bechar; biotechnol; bsn; capacity; carica; chemistry; compounds; content; correlation; ctl; des; determination; differences; different; dpph; dry; extracts; ficus; fig; figs; figure; flavonoids; flowers; food; free; fruits; gae; high; human; ic50; inhibition; journal; level; mascara; medicinal; meoh; meoh var.2; merah; messaoudi; methanolic; min; nepal; njb; phenolic; phytochemicals; plant; positive; radical; samples; scavenging; significant; solution; species; tac; tannins; test; tfl; total; total antioxidant; tpl; value; var.2; varieties; variety; weight
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- njb-178
- author: Hori, Kengo; Devkota, Hari Prasad
- title: Phenolic Compounds from the Aerial Parts of Adenophora triphylla (Thunb.) A. DC. var. triphylla and their Free Radical Scavenging Activity
- date: 2020-07-31
- words: 2692
- flesch: 63
- summary: japonica (Regel) H. Hara (“Tsuriganeninjin” in Japanese) are among many varieties of plant A. triphylla (Thunb.) Free radical scavenging activity of the extract and compounds was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity method.
- keywords: acid; activities; activity; adenophora; aerial; chemical; compounds; devkota; dpph; extract; flavonoids; foods; free; functional; gel; isolated; japan; japonica; kumamoto; leaves; lh-20; luteolin; meoh; nepal; nmr; parts; phenolic; plant; potential; radical; research; scavenging; sephadex; study; table; thunb; triphylla; triphylla var; var
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- njb-180
- author: Rai, Shyamala; Karki, Barsha; Humagain, Sujita; Rimal, Sandesh; Adhikari, Sandhya; Adhikari, Shilpa; Thapa, Suchitra
- title: Antibiogram of Escherichia coli and Staphylococcus aureus Isolated from Milk Sold in Kathmandu District
- date: 2020-12-30
- words: 3861
- flesch: 52
- summary: [7, 11] in milk samples but in the case of Nepal very less studies are found on antibiotic resistance of milk isolates. Total bacterial count of milk samples The collected milk samples were serially diluted up to- 10-8 dilution.
- keywords: acid; ampicillin; analysis; antibiogram; antibiotic; antimicrobial; aureus; cefoxitin; ceftriaxone; cfu; chloramphenicol; ciprofloxacin; coli; dec; distribution; district; e. coli; esbl; escherichia; figure; food; health; higher; infections; isolates; journal; kathmandu; load; mdr; medium; microbial; microbiology; milk; milk samples; mrsa; nalidixic; nepal; pasteurized; pathogenic; presence; prevalence; quality; raw; raw milk; resistance; s. aureus; samples; standard; staphylococcus; studies; study; susceptibility; tbc; tetracycline; total; yes
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- njb-182
- author: Devkota, Surya Prasad; Paudel, Ashmita; Gurung, Krishna
- title: Vancomycin Intermediate MRSA Isolates Obtained from Retail Chicken Meat and Eggs Collected at Pokhara, Nepal
- date: 2019-12-29
- words: 3803
- flesch: 49
- summary: S aureus was isolated from eggshells and chicken meat samples collected from different retail outlets of the Pokhara metropolitan. Isolated yellow colonies were further examined by Gram-staining, catalase, and coagulase test to detect S aureus.
- keywords: agar; animal; antibiotic; antimicrobial; aureus; biotechnology; chicken; china; clsi; concentration; detection; devkota; egg; eggs; erythromycin; food; health; humans; inoculated; intermediate; isolates; items; journal; meat; methicillin; mic; mrsa; mrsa isolates; mssa; nepal; nepjol.info/index.php/njb; njb; pokhara; prevalence; range; resistance; retail; s aureus; samples; sensitive; society; staphylococcus; staphylococcus aureus; sterile; study; susceptibility; table; test; use; value; vancomycin; vol
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- njb-189
- author: Chhetri, Til Kumari; Subedee, Bijay Raj; Pant, Bijaya
- title: Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability
- date: 2019-12-29
- words: 7065
- flesch: 62
- summary: Increasing the efficiency of the use of available soil nitrogen can meet the additional Nitrogen demand by making cereal plants capable of fixing its own nitrogen through close Nepal Journal of Biotechnology. Apart from direct benefit from effective nitrogen fixation [4] legumes and Rhizobium provides added value in weed, pathogen and insect control when rotated with crop in farming system [5] together with improving soil structure and increasing soil organic matter content
- keywords: agar; agricultural; air; alginate; average; bacteria; beads; beaker; biochemical; biological; biotechnology; bradyrhizobium; broth; cells; chhetri; colonies; colony; color; concentration; congo; crop; culture; days; different; dilution; disc; effective; encapsulation; environmental; fixation; fixing; form; growth; hours; immobilized; inoculants; inoculated; inoculation; inoculums; japonicum; journal; legumes; mass; media; minutes; months; nacl; nepal; nepjol.info/index.php/njb; nitrogen; nitrogen fixation; njb; nodules; number; organism; peat; plant; plates; prepared; present; production; pure; research; result; rhizobia; rhizobium; root; rotation; seed; slow; society; sodium; soil; solution; species; sterile; strain; study; sucrose; surface; survival; test; tolerance; use; viability; viable; vol; water; yema
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- njb-190
- author: Bhandari, Sudip; Rayamajhee, Binod; Dhungel, Laxmi; Poudel, Sami; Gaire, Bhagwati; Shrestha, Sunil; Parajuli, Niranjan
- title: Infectious sources of Histoplasmosis and molecular techniques for its identification
- date: 2019-12-29
- words: 4230
- flesch: 56
- summary: Notably, in tuberculosis- endemic regions, disseminated histoplasmosis can easily be misdiagnosed as tuberculosis because of its similar clinical symptoms and must be considered as histoplasmosis infection in patients who do not respond to empiric anti- tubercular therapy [20]. The real-time RT-PCR or qPCR shows promising results in the diagnosis of histoplasmosis infection, which currently identified the H. capsulatum among several other fungi
- keywords: aids; assay; bhandari; biotechnology; body; capsulatum; cases; clinical; country; daily; detection; diagnosis; disease; dissemination; dna; endemic; form; fungal; fungus; health; high; histoplasma; histoplasmosis; hiv; identification; immune; india; infection; journal; kauffman; lack; med; microbiol; molecular; mycelial; national; nepal; nepjol.info/index.php/njb; njb; number; organ; patients; pcr; people; population; probe; progressive; proper; public; pulmonary; reaction; real; research; review; risk; samples; sector; severe; significant; society; soil; spores; strategies; symptoms; technique; time; transplant; treatment; update; vol; wheat; years; yeast
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- njb-195
- author: Subedi, Deepak; Devkota, Surya Prasad; Bhatta, Dharm Raj; Sharma, Binita Koirala; Paudel, Ashmita; Gurung, Krishna; Gautam, Damodar
- title: Antibiotic Susceptibility Profile of respiratory pathogens obtained at tertiary care hospital from western Nepal
- date: 2019-12-29
- words: 3314
- flesch: 47
- summary: Keywords: respiratory pathogens, western Nepal, Pseudomonas aeruginosa, Acinetobacter spp, Klebsiella pneumonia, S. aureus. Surveillance study on respiratory pathogens was imminent in this part of the nation.
- keywords: acinetobacter; aeruginosa; antibiotic; antimicrobial; aureus; biotechnology; candida; care; clindamycin; colistin; drug; erythromycin; gram; hospital; infections; int; isolates; journal; klebsiella; lower; med; microbiol; negative; nepal; nepjol.info/index.php/njb; njb; pathogens; patients; pneumoniae; pokhara; positive; profile; pseudomonas; pyogenes; resistance; respiratory; samples; sensitive; society; spp; sputum; staphylococcus; streptococcus; study; subedi; susceptibility; table; tertiary; test; throat; tract; vancomycin; vol
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- njb-2
- author: Shrestha, Bijay Kumar; Shakya, Jenish
- title: Simple method devised for rapid isolation and identification of Vibrio cholerae from water resources of Sunsari District, Nepal
- date: 2021-12-30
- words: 4313
- flesch: 57
- summary: This study aimed to study the incidence of Vibrio species and employ simple method for rapid detection of Vibrio cholerae from water samples of Sunsari, Nepal. Therefore, the aim of this study was to study the incidence of Vibrio species and devise simple method for rapid detection of Vibrio cholera from water samples of Sunsari, Nepal.
- keywords: agar; alginolyticus; alkaline; arginine; biochemical; biotechnol; bsn; chloride; cholerae; citrate; collected; colonies; colony; contamination; culture; devoid; different; diseases; distribution; district; doi; drinking; environment; fermenting; fluvialis; health; hours; identification; incidence; isolated; isolation; journal; media; method; microbiology; nacl; nepal; njb; non; nutrient; outbreak; oxidase; pond; presumptive; public; rapid; resource; samples; sewage; shakya; shrestha; simple; sodium; species; strains; study; sucrose; sunsari; table; tap; tcbs; test; tube; vibrio; vibrio cholerae; water; water samples; yellow
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- njb-203
- author: Suwal, Shyam; Marciniak, Alice
- title: Technologies for the Extraction, Separation and Purification of polyphenols – A Review
- date: 2019-01-15
- words: 9372
- flesch: 51
- summary: ©NJB, Biotechnology Society of Nepal 81 Nepjol.info/index.php/njb on polyphenols extraction but considerable innovations are needed for their industrial and economic feasibility. The irradiation of plant tissues with ultrasound increase the number of extraction solvent exposed to the molecules by breaking down the cell wall, increasing swelling and hydration degree thus improving the diffusion and mass transfer [19].
- keywords: 2010; acids; activity; addition; anthocyanins; antioxidant; application; assisted; autheor; bazinet; bioactive; biotechnology; cell; chem; chemical; chromatogr; citrus; comparison; compounds; concentration; content; conventional; cost; cranberry; degradation; different; diseases; distillation; e c; effect; efficiency; electric; electrodialysis; eng; et al; ethanol; exchange; extraction; extraction process; field; figure; filtration; flavonoids; fluid; food; functional; grape; green; health; heat; high; higher; hydrostatic; iec; increase; industrial; interest; journal; juice; known; leaves; liquid; low; mae; material; membrane; methods; microwave; min; molecular; molecules; nepal; nepjol.info/index.php/njb; njb; non; number; olive; organic; parameters; pef; phenolic; plant; polyphenols; present; pressure; pressurized; process; processing; product; pulses; purification; quality; rate; recovery; red; review; sample; sci; seeds; selectivity; separation; sfe; society; solid; solvent; solvent extraction; sources; soxhlet; study; supercritical; surname; system; tea; technique; technologies; temperature; thermal; time; total; treatment; type; uae; ultrafiltration; ultrasound; use; vol; water; wine
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- njb-204
- author: Shrestha, Dhiraj; Bhattachan, Balkrishna
- title: Nipah Virus (NiV) Infection: Is Nepal Prepared for the Possible Outbreak?
- date: 2019-01-15
- words: 2696
- flesch: 59
- summary: Thus, the territorial habitat of these bats is the high risk zone of NiV outbreak. 23 Dawes BE, Kalveram B, Ikegami T, Juelich T, Smith JK, Zhang L, Park A, Lee B, Komeno T, Furuta Y, Freiberg AN: Favipiravir (T-705) protects against Nipah virus infection in the hamster model.
- keywords: available; bats; bhattachan; biotechnology; blueprint; cases; disease; distribution; doi; encephalitis; family; health; high; host; human; ifn; india; infection; journal; model; mortality; natural; nepal; nepjol.info/index.php/njb; nipah; nipah virus; niv; njb; organization; outbreak; pigs; pmid; possible; prevention; protection; proteins; r&d; research; respiratory; risk; shrestha; signaling; society; symptoms; threat; transmission; vaccines; virol; virus; vol; world
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- njb-205
- author: Thapa, Christina; Shakya, Pallavi; Shrestha, Rabina; Pal, Sushovita; Manandhar, Prakash
- title: Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB
- date: 2019-01-15
- words: 3576
- flesch: 50
- summary: ©NJB, Biotechnology Society of Nepal 62 Nepjol.info/index.php/njb Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB Christina Thapa¹, Pallavi Shakya¹, Rabina Shrestha¹, Sushovita Pal¹, Prakash Manandhar² Department of Biotechnology, SANN International College, Purbanchal University, Nepal1 Department of Microbiology, St. Xavier’s College, Nepal2 Abstract Polyhydroxybutyrates (PHBs) are energy reserves synthesized by different micro-organisms such as Alcaligenes, Pseudomonas, Staphylococcus, Algae, in excess of carbon and limitation of nutrients like nitrogen. The micro-organisms for this purpose were isolated from 4 different soil samples and screened for PHB production.
- keywords: accumulation; agar; analysis; arthrobacter; bacillus; bacteria; biotechnology; black; broth; carbon; characterization; concentration; conditions; culture; different; extraction; figure; ftir; glucose; growth; hours; incubation; isolates; isolation; journal; luteus; media; micrococcus; morphological; nacl; nepal; nepjol.info/index.php/njb; njb; optimization; pasteurii; phb; plastics; positive; production; research; samples; showing; society; source; species; spp; staining; strains; sudan; test; vol
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- njb-21
- author: Sah, Sunil Kumar; Yadav, Naval Kishor; Kurmi, Roshan; Rauniyar, Ramanuj; Manandhar, Krishan Das; Gupta, Birendra Prasad
- title: Pattern of Cancer in Nepal from 2003 to 2011
- date: 2016-12-31
- words: 2711
- flesch: 64
- summary: The total number of cancer cases were increasing from 2003 to 2011 and it become double in 2011.. Out of 75 district of Nepal, more number of cancer cases was found in Kathmandu, Sunsari, Morang, Chitwan, Lalitpur, Jhapa, Kaski, Nawalparasi, Rupendehi and Kavrepalchowk in 2010.
- keywords: age; biotechnology; breast; cancer; cancer cases; cases; chitwan; common; diagnosis; distribution; examination; female; figure; group; hospital; iarc; journal; kathmandu; kurmi; lung; male; nepal; nepjol.info/index.php/njb; njb; number; religion; society; study; table; total; vol; years
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- njb-212
- author: Bhattachan, Balkrishna; Rai, Ganesh; Munankarmi, Nabin Narayan; Sherchand, Jeevan Bahadur
- title: Cyclospora cayetanensis: An Infestation among Diarrheal Children in Kathmandu Valley, Nepal
- date: 2019-01-15
- words: 2267
- flesch: 63
- summary: ©NJB, Biotechnology Society of Nepal 16 Nepjol.info/index.php/njb Cyclospora cayetanensis: Orozco-Mosqueda GE, Martı´nez-Loya OA, and Ortega YR: Cyclospora cayetanensis in a Pediatric Hospital in Morelia, Mexico.
- keywords: age; august; biotechnology; cayetanensis; children; cross; cryptosporidium; cyclospora; diarrheal; female; higher; highest; hospital; infection; intestinal; journal; june; kanti; kathmandu; med; medicine; nepal; old; parasites; patients; prevalence; rate; research; samples; september; society; stool; study; table; total; water; year
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- njb-213
- author: Nepal, Pragya; Singh, Minu; Baniya, Amina; Singh, Sushma; Sainju, Hari Krishna; Shrestha, Rajani
- title: Comparative Antioxidant, Antimicrobial and Phytochemical Assesments of Leaves of Desmostachya bipinnata L. Stapf, Hordeum vulgare L. and Drepanostachyum falcatum (Nees) Keng f.
- date: 2019-01-15
- words: 5084
- flesch: 51
- summary: Plant extracts used in different … H.vulgare D.bipinnata D.falcatum 0 5 10 15 20 25 Pure 2:01 1:01Z o n e o f In h ib it io n ( in m m ) Plant extracts used in different concentrations H.vulgare D.bipinnata D.falcatum 0 5 10 15 20 25 pure 2:01 1:01 Z o n e o f In h ib it io n (i n m m )
- keywords: + +; absorbance; acid; activity; alkaloids; analysis; antifungal; antimicrobial; antioxidant; assay; aureus; bacteria; biotechnology; bipinnata; compounds; concentration; coumarins; desmostachya; different; dpph; drepanostachyum; drepanostachyum falcatum; extracts; falcatum; figure; glycosides; graph; hordeum; hordeum vulgare; inhibition; journal; keng; kumar; medicinal; methanol; nees; nepal; nepjol.info/index.php/njb; njb; phytochemical; plants; positive; presence; present; pure; radical; religious; result; salmonella; sample; saponins; scavenging; screening; society; solution; sterols; table; test; use; vol; vulgare; zoi; zone
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- njb-214
- author: Saiju, Hari Krishna; Bajracharya, Abhishesh; Rajbahak, Brishav; Ghimire, Stuti
- title: Comparative Study of Growth Statistics of Two Species of Paulownia and Optimization of Rooting Methods
- date: 2019-01-15
- words: 3267
- flesch: 68
- summary: Keywords: Paulownia tomentosa, Paulownia fortuneii, growth comparison, in-vitro rooting, sand- rooting, nodal culture *Corresponding Author Email: hk.saiju@aitm.edu.np Introduction Paulownia is a fast-growing, woody tree native to the forests of China [1]. Steud and Paulownia fortuneii (Seem.)
- keywords: average; average root; biotechnology; comparison; concentrations; culture; density; development; figure; fortuneii; growth; higher; incubation; l naa; length; medium; naa; nepal; number; optimization; paulownia; plants; rate; room; root; rooting; samples; sand; species; study; table; tomentosa; total; vessels; week; wood
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- njb-22
- author: Thapa, Nita; Thapa, Pratiksha; Bhandari, Jay; Niraula, Prasodhan; Shrestha, Nikita; Shrestha, Bhupal G
- title: Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus
- date: 2016-12-31
- words: 3568
- flesch: 57
- summary: ©NJB, Biotechnology Society of Nepal 52 Nepjol.info/index.php/njb IC50 value of ascorbic acid = 5.62µg/ml IC50 value of Artocarpus heterophyllus = 8.33µg/ml IC50 value of ascorbic acid = 12.96µg/ml IC50 value of Artocarpus heterophyllus = 29.29µg/ml IC50 value of Ascorbic acid=5.27µg/ml IC50 value of Artocarpus heterophyllus=10.69µg/ml Figure 2: A, B and C represents antioxidant activity of methanol, ethyl acetate, aqueous methanol respectively. topic of antimicrobial plant extracts as they believe that these phytochemicals will find their way as alternate to antimicrobial drugs as those prescribed by physicians. Further, antimicrobial activity was assessed through disc diffusion method with six pathological bacteria and two fungi strains in four different concentrations of plant extract.
- keywords: 1ml; 200mg; acetate; acid; activity; antimicrobial; antioxidant; aqueous; artocarpus; ascorbic; biotechnology; cancer; coumarin; dpph; ethyl; extract; extraction; flavus; heterophyllus; ic50; inhibition; journal; leaf; medicinal; methanol; nepal; nepjol.info/index.php/njb; njb; phytochemical; plant; presence; rhizopus; sample; saponin; shrestha; society; solution; solvent; standard; study; subtilis; table; test; thapa; traditional; use; value; vol; yield; zoi
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- njb-224
- author: Balzer, Anna; Shah, Sujit; Kumar Ale, Yam; Adhikari, Dipti; Niroula, Sanjit; Mandal, Jawaharlal; Bahadur Pun, Amar; Poudel, Krishna; Bhantana, Parashuram; Timsina, Govinda; Karn, Ravindra; Karki, Sujwal
- title: Development of Effective Protocol for Four Varieties of Large Cardamom
- date: 2022-07-30
- words: 3871
- flesch: 65
- summary: ©NJB, BSN 3 Table 1: Growth patterns of Dambarsai, Golsai, Sikkimae, and Ramsai cardamom varieties in response to differing cytokinin concentration in micropropagation. Table 2: Growth ranking for optimal shooting and rooting of Dambarsai, Golsai, Sikkimae, and Ramsai cardamom varieties in response to differing cytokinin concentration in micropropagation.
- keywords: 0.5mg; 1mg; acclimatization; amomum; assay; author; bap; biotechnol; cardamom; cocopeat; concentration; culture; cytokinin; dambarsai; days; development; different; disease; field; golsai; growth; high; iba; l bap; l iba; large; length; media; micropropagation; moss; nepal; number; optimal; plant; production; protocol; quality; ramsai; ranking; research; root; roxb; sawdust; shoot; sikkimae; soil; study; substrate; subulatum; table; tissue; transfer; varieties; variety
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- njb-225
- author: Prasai, Shristi; Shrestha, Puja; Pandey, Sriniwas; Adhikari, Ishika; Gurung, Srijana; Prajapati, Kamil
- title: Microbial Quality Assessment of Raw Freshwater Fish Sold in Local Markets of Kathmandu Valley
- date: 2022-07-30
- words: 4746
- flesch: 64
- summary: It was then serially diluted up to a dilution of 10-5. Bacteriological analysis of fish samples For total plate count (TPC), 0.1ml sample from 10-3 and 10-5 dilutions were taken and spread plating was done on PCA agar. Average TPC of fish samples Sample Organs (cfu/gm in average) Skin Gills Intestine Average LR 6.5 x 106 8.2 x 107 8.05 x 107 5.6 x 107 CC 1.23 x 107 2.96 x 107 8.03 x 107 4.1 x 107 CB 1.2 x 107 6.6 x 107 2.3 x 108 1.02 x 108 Average 1.02 x 107 5.9 x 107 1.3 x 108 Note: LR = Labeo rohita, CC = Cyprinus carpio and CB = Clarias batrachus In this study, 9 fish sample of 3 different varieties, L. rohita (LR), C. carpio (CC) and C. batrachus (CB) were analyzed for its microbial quality and antimicrobial susceptibility testing was done for the isolated strain.
- keywords: aureus; average; bacteria; batrachus; c. carpio; carpio; cfu; cholerae; coli; coliform; cons; contaminated; count; cyprinus; different; fecal; fish; fishes; food; freshwater; gills; gram; highest; intestine; isolated; kathmandu; labeo; load; markets; microbial; nepal; paratyphi; pathogenic; presence; present; quality; raw; rohita; salmonella; samples; sci; skin; spp; staphylococcus; study; table; tcc; total; tpc; typhi; vibrio
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- njb-226
- author: Habiba Akter, Umme; Begum, Fatema; Islam, M. R.; Nahar Prinky, Jannatun; Khatun, Mst. Rehena
- title: Occurrence of Purple Blotch Disease Associated with Selected Garlic Varieties and its Management Through Bio-Agent, Botanicals and Fungicides
- date: 2022-07-30
- words: 8468
- flesch: 65
- summary: The current research work was aimed to assess the occurrence of purple blotch disease incidence and severity of selected garlic varieties, to isolate and identify purple blotch disease and pathogenicity test of Alternaria porri, and its management using bio-agent, plant extracts and fungicides. BARI Rashun-3 showed the highest disease incidence (40.00%) and severity (92.00%) of purple blotch disease.
- keywords: agar; agent; agricultural; akter; allium; alternaria; alternaria porri; bangladesh; bari; bau; bau1; bau2; bio; biotechnol; black; blotch; blotch disease; botanicals; brown; bsn; camara; colony; color; condition; control; correlation; das; data; days; different; disease; disease incidence; disease severity; effective; ellis; evaluation; extract; fluffy; fresh; fungicides; garlic; growth; harzianum; identification; incidence; indian; inoculation; int; islam; isolates; lantana; leaf; leaves; lesions; level; lind; local; lowest; management; maximum; media; morphological; nepal; njb; onion; pathogenicity; pathogens; pda; plant; porri; production; purple; purple blotch; rashun-3; regular; research; results; sci; severity; similar; soil; studies; symptoms; table; test; treatment; trichoderma; university; varieties; variety; water; weight; white; yield
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- njb-227
- author: Kumar Shrestha, Bijay; Dhungana, Bidhya; Shakya, Jenish; Shrestha, Romika; Chauhan, Sujata
- title: Phytochemical and Antimicrobial Screening of Bark Extract of Shorea robusta (Sal)
- date: 2022-07-30
- words: 4546
- flesch: 51
- summary: Antimicrobial Screening of bark extract of S. robusta against E. coli Figure 3: Antimicrobial susceptibility test by S. robusta extract. In one study, S. robusta extract exhibited antibacterial activity against different clinical pathogens
- keywords: acid; activity; agar; alkaloids; anthraquinone; antibacterial; antimicrobial; aureus; ayurvedic; bacterial; bark; bark extract; biotechnol; bsn; campus; central; clinical; coli; color; concentration; cultural; culture; development; different; diseases; doi; drug; ethanolic; ethanolic bark; ethanolic extract; extract; flavonoids; gaertn; growth; himedia; india; infections; information; inhibition; inhibitory; inoculated; journal; medicinal; minimum; nepal; njb; pathogens; phlobatannins; phytochemical; plant; plates; presence; present; proteins; research; resistance; robusta; robusta bark; s. robusta; sample; screening; shorea; shorea robusta; shrestha; solution; steroids; strains; study; suspension; table; tannins; technology; terpenoids; test; traditional; zone
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- njb-228
- author: Bajgain, Abhishek; Dev Neupane, Bashu; Sarraf, Diwakar; Karmacharya, Jwalant; Ranjitkar, Saksham; Shrestha, Rajan; Gyawali, Rajendra
- title: Quality Evaluation of Apis laboriosa and Apis mellifera Honey Collected from Bagmati Province, Nepal
- date: 2022-07-30
- words: 4502
- flesch: 65
- summary: Various values of pH shown by different honey samples are tabulated down in Table 4. Honey samples of Apis laboriosa and Apis mellifera were collected during spring of 2019 & 2021 and autumn 2021 from the Bagmati province, Nepal.
- keywords: + +; absorbance; acid; anti; apis; autumn; bajgain; biotechnol; bsn; colour; concentration; content; different; dpph; figure; flow; honey; honey samples; index; instrument; laboriosa; manufacturer; moisture; nectar; nepal; njb; optical; oxidant; phenolic; positive; potency; ppm; properties; quercetin; refractive; rheological; rotation; rpm; s.n; samples; sand; specific; spring; standard; table; test; tfc; time; total; tpc; viscosity; water
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- njb-229
- author: Saud, Sarada; Agrawal, Ashwani; Pokhrel, Soniya; Subedi, Sushma; Shrestha, Sanjit; Man Amatya, Niroj
- title: Screening of Carbapenem Resistance Klebsiella pneumoniae and its MIC against Imipenem
- date: 2022-07-30
- words: 3321
- flesch: 44
- summary: Therefore, this cross-sectional study was conducted in B & B Hospital, Lalitpur to screen the distribution of carbapenem resistance K. pneumoniae through ertapenem and to assess the minimum inhibitory concentration of imipenem for screened carbapenem resistance K. pneumoniae. Shilpa K, Thomas R, Ramyashree A. Isolation and Antimicrobial sensitivity pattern of Klebsiella pneumoniae from sputum samples in a tertiary care hospital.
- keywords: antibiotic; antimicrobial; bacterium; biotechnol; blood; bsn; carbapenem; carbapenemase; ceftriaxone; ciprofloxacin; clinical; clsi; control; crkp; different; disc; enterobacteriaceae; ertapenem; european; gentamicin; global; health; hospital; imipenem; india; infection; intermediate; isolates; klebsiella; mic; microbiology; nepal; njb; non; number; pathogen; patients; pneumoniae; range; rate; resistance; samples; saud; screening; sensitive; study; surveillance; susceptibility; susceptible; table; test
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- njb-23
- author: Sharma, Manisha; Pant, Narayan Dutt; Pandey, Pratikshya
- title: Prevalence of Non-Albicans Candida Among the Patients Attending a Tertiary Care Hospital in Kathmandu, Nepal
- date: 2016-12-31
- words: 2488
- flesch: 49
- summary: Among them, Candida glabrata, Candida parapsilosis and Candida tropicalis are non-albicans Candida of increasing clinical significance [5]. Abstract The main objective of this study was to determine the prevalence of non-albicans Candida among the patients attending a tertiary care hospital in Kathmandu, Nepal.
- keywords: albicans; albicans candida; biotechnology; candida; candida spp; care; clinical; deorukhkar; fungal; glabrata; hospital; identification; immunocompromised; infection; isolated; isolation; kathmandu; nepal; non; patients; prevalence; rate; samples; secretion; species; spp; sputum; study; swab; test; total; tropicalis; urine; vaginal
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- njb-239
- author: Kumar Shrestha, Deepak; Kumar Sapkota, Basanta; Prasad Sharma, Krishna
- title: Assessment of Phytochemicals, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Selected Nepalese Medicinal Plants
- date: 2022-12-31
- words: 4837
- flesch: 56
- summary: Polyphenols are the strong antioxidant in plant extracts [11]. This test is a straightforward, popular, and widely accepted method to assess the antioxidant potential of plant extracts.
- keywords: + +; absorbance; acid; activities; activity; adhatoda; aegle; alkaloids; analysis; antioxidant; arbortristis; ascorbic; biotechnol; bsn; calibration; chloride; ciocalteu; concentration; content; crateva; curve; different; dioica; dpph; evaluation; extract; flavonoid; folin; free; gae; gallic; highest; ic50; journal; justicia; lowest; marmelos; mean; medicinal; metabolites; methanolic; method; natural; nepal; njb; nyctanthes; oxidative; phenolic; phytochemical; plants; polyphenols; present; quercetin; radical; reagent; research; results; sample; scavenging; shrestha; sodium; solution; standard; study; table; test; total; tpc; unilocularis; urtica; value; weight
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- njb-24
- author: Shrestha, Kushal; Shrestha, Smita; Khatiwada, Saroj; Acharya, Bishu; Manandhar, Sulochana; Pokharel, Rohit Kumar
- title: Multiplex Ligation Dependent Probe Amplification Based Mutation Analysis of Dystrophin Gene in Nepalese Patients with Duchenne Muscular Dystrophy
- date: 2016-12-31
- words: 3087
- flesch: 58
- summary: A commercial MLPA kit (MRC Holland) with probes of P034 (DMD exons 1-10, 21-30, 41-50, and 61-70) and P035 (DMD exons 11-20, 31-40, 51-60, and 71-79) was used to detect DMD deletion/duplication in DMD patients according to the manufacturer’s recommended protocol (Amsterdam, Netherlands). Wang X, Wang Z, Yan M, Huang S, Chen TJ, Zhong N: Similarity of DMD gene deletion and duplication in the Chinese patients compared to global populations.
- keywords: age; amplification; analysis; assay; biotechnology; bmd; cases; clinical; deletion; dependent; detection; diagnosis; dmd; dna; duchenne; duplications; dystrophin; dystrophin gene; exon; exonic; gene; genetic; journal; level; ligation; mlpa; multiplex; muscular; mutation; nepal; nepalese; nepjol.info/index.php/njb; njb; number; p034; p035; parents; patients; probe; regions; samples; shrestha; society; study; test; tribhuvan; university; vol; years
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- njb-240
- author: Gyawali, Rajendra; Paudel, Aparna; Lamsal, Babita; Bhatta, Pranisha; Maharjan, Albina; Khaitu, Nina; Shrestha, Rajan
- title: Comparative Study on Antioxidant Activity of Propolis of Apis mellifera from Different Regions of Nepal
- date: 2022-12-31
- words: 2987
- flesch: 53
- summary: Propolis samples Total Phenolic Content Nepal J Biotechnol. It is concluded that there is no direct correlation on total antioxidant property of propolis with their total phenolic and total flavonoid content among collected samples.
- keywords: absorbance; acid; activity; antibacterial; antioxidant; assay; bee; biological; biotechnol; capacity; chemical; collected; composition; compounds; content; different; doi; dpph; equivalent; extract; figure; flavonoid; free; gae; gallic; gram; gyawali; highest; ic50; journal; method; nepal; nepalese; njb; origin; phenolic; plant; properties; property; propolis; quercetin; radical; research; samples; scavenging; shrestha; spl; standard; study; total; value
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- njb-241
- author: Rehena, M. K.; Aminuzzaman, F. M.; Ashrafi, M. L.; Chowdhury, M.S.M.; Habiba, U. A.; Nazifa, Z.; Ahmed, M.
- title: Efficacy of Ethanol Extract of Botanicals in Controlling Wheat Blast Fungus Magnaporthe oryzae triticum in vitro
- date: 2022-12-31
- words: 4826
- flesch: 61
- summary: The effect of plant extract was calculated as percent growth inhibition using the following formula as adopted by [22, 23]. Effects of plant extracts on controlling wheat blast disease caused by Magnaporthe oryzae Pathotype triticum in Bangladesh.
- keywords: 1:0.25; allamanda; allium; aloe; aloe vera; azadirachta; bangladesh; black; blast; botanicals; cathartica; cepa; colony; concentration; control; culture; cumin; curcuma; dai; days; different; disease; ethanol; extracts; food; garlic; growth; highest; indica; inhibition; leaf; longa; magnaporthe; media; mot; mycelial; nepal; nigella; officinale; oryzae; pathogen; pda; plant; plates; radial; regular; research; rice; sativa; sativum; seed; table; triticum; vera; vitro; wheat; white; zingiber
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- njb-242
- author: Chinenye Onwubiko, Nwakuche; Ifeanyi Uguru, Michael; Ovute Chimdi, Grace
- title: Molecular Genetic Diversity in Bambara groundnut [Vigna subterranea (L.) Verdc] Assessed by Microsatellite Markers
- date: 2022-12-31
- words: 3870
- flesch: 56
- summary: In fact, there was a report from a previous study on a similar trend of association between Bambara groundnut accessions collected from different geographical areas, and in that study, it was concluded that these accessions were either related, or the genotypes were the same (22). 2 0 2 2 D e c ; 1 0 (2): 85-90 Research article DOI: https://doi.org/10.54796/njb.v10i2.242 ©NJB, BSN 85 Molecular Genetic Diversity in Bambara groundnut [Vigna subterranea (L.) Verdc]
- keywords: accessions; analysis; areas; arithmetic; bambara; bambara groundnut; biotechnol; bsn; characters; cluster; crop; dendrogram; different; diversity; dna; gel; genetic; genetic diversity; genotypes; geographical; groundnut; groundnut accessions; group; improvement; jaccard; journal; landraces; level; markers; mean; minutes; molecular; morphological; nepal; nigeria; njb; onwubiko; origin; plant; polymorphism; report; result; selection; similarity; species; ssr; study; subterranea; supernatant; togo; tubes; tvsu; variation; verdc; vigna; vigna subterranea
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- njb-243
- author: Aththanayake, A.M.K.C.B.; Rathnayake, I.V.N.; Deeyamulla, M.P.
- title: Detoxification and Removal of Hexavalent Chromium in Aquatic Systems: Applications of Bioremediation
- date: 2022-12-31
- words: 14927
- flesch: 43
- summary: Adsorption is widely used for chromium removal in wastewater, consisting of Nepal J Biotechnol. This study also indicates 65% of chromium removal from tannery effluent and 85% of Cr(VI) removal from the synthetic medium at pH 6 within 07 days.
- keywords: accumulation; acidic; acinetobacter; adsorbent; adsorption; adsorption cr(vi; agents; air; algae; application; approach; apr; aquatic; aqueous; arthrobacter; aspergillus; aththanayake; attachment; aug; authorities; available; bacillus; bacterial; beads; biodegrad; biodeterior; biofilm; biological; biomass; bioreduction; bioremediation; bioresour; biosorption; biotechnol; bsn; calcium; cancer; carbon; cell; cellulosimicrobium; cerevisiae; changes; characterization; chemical; chemosphere; chen; chlorella; chromate; chrome; chromium; chromium reduction; chromium removal; chromium(vi; coli; community; components; compounds; concentration; conditions; consortia; contaminated; cost; cr(iii; cr(vi; crb; crit; damages; das; days; dec; desalination; detoxification; different; discharge; disposal; editors; effective; effects; efficient; effluent; electron; electroplating; eng; eng j; environ; environ sci; environmental; eps; escherichia; et al; exchange; exposure; factors; feb; filtration; formation; forms; free; friendly; fungal; fungi; genotoxic; glass; green; growth; hazard; health; heavy; hexavalent; hexavalent chromium; high; higher; hrs; https://linkinghub.elsevier.com/retrieve/pii/; husk; immobilized; industrial; industry; inhibition; initial; int j; international; internet; isolated; j environ; j hazard; jan; jul; jun; kelaniya; kumar; leather; lett; level; liu; living; low; mar; marine; mater; materials; mechanisms; membrane; metal; methods; microbiol; microorganisms; mixed; mobility; montmorillonite; nanocomposite; nanoparticles; natural; nature; nepal; nepal j; new; njb; nov; occupational; oct; optimization; oxidation; oxide; park; physical; physiological; phytoremediation; pigment; planktonic; plants; point; pollut; pollutants; potential; presence; preservation; process; processes; pseudomonas; range; raw; reduction; reduction cr(vi; remediate; remediation; removal; requirements; res; research; resins; resistance; response; review; saccharomyces; saha; scale; sci; sep; shewanella; silva; site; skin; soil; solubility; solutions; sorokiniana; sources; species; springer; stainless; staphylococcus; states; steel; strain; stress; studies; study; subtilis; surface; synthetic; synthetic wastewater; system; table; tannery; tanning; techniques; technologies; technology; temperature; textile; time; tolerance; toxic; toxicity; toxicol; treatment; trichoderma; trivalent; type; ulva; uptake; vulgaris; wang; wastes; wastewater; wide; wood; yeast; zhang
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- njb-25
- author: Amgai, Resham Babu; Prasai, Hari Kumar; Pandey, Yama Raj
- title: Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco) Micro-propagation
- date: 2016-12-31
- words: 2251
- flesch: 64
- summary: MS basal media+4mg/l BAP+0.2mg/l IAA don’t produce any shooting directly from immature shoot with single bud (Table 6). 3. Sharma S, Singh B, Rani G, Zaidi AA, Hallan V, Nagpal A, Virk GS: Production of Indian citrus ringspot virus free plants of Kinnow employing chemotherapy coupled with shoot tip grafting.
- keywords: agriculture; bap; basal; biotechnology; bud; callusing; citrus; composition; culture; different; explant; flower; grafting; highest; iaa; immature; incubation; mandarin; mean; media; micro; nepal; observed; orange; shooting; single; table; test; tip
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- njb-26
- author: Kurmi, Roshan; Rauniyar, Ramanuj; Manandhar, Krishna Das; Gupta, Birendra Prasad
- title: Evaluation of the XpertMTB/RIF for the Diagnosis of Pulmonary Tuberculosis Among the Patients Attending DOTS Center Parsa District of Nepal
- date: 2016-12-31
- words: 4235
- flesch: 55
- summary: [1]. DOTS program was initiated in April 2001 with a goal to diagnose and cure TB patients which has treated more than 20 thousand patients till date [2]. Gene Xpert MTB RIF Pattern in TB patient and MDR Pattern in TB Patients are shown in Figure 2 and Figure 3 respectively.
- keywords: analysis; assay; biotechnology; cases; centres; chest; clinical; combined; cough; culture; development; diagnosis; district; dots; figure; genexpert; government; health; higher; hiv; journal; kurmi; low; mdr; microscopy; mtb; national; negative; nepal; nepjol.info/index.php/njb; njb; number; parsa; patients; positive; possible; predictive; prevalence; program; pulmonary; rapid; region; resistant; results; rif; sensitivity; similar; smear; society; specificity; sputum; study; table; test; treatment; tuberculosis; uncentrifuged; value; vol
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- njb-27
- author: Shrestha, Rupendra; Khadka, Sundar; Wagle, Susbin Raj; Sapkota, Alisha
- title: Detection of Latent HIV-1 Infection and Drug Resistant Mutation Testing in Nepal: HIV-1 env V3 DNA Sequence and RT Gene (M184V) Mutation
- date: 2016-12-31
- words: 4705
- flesch: 59
- summary: In addition, there have been no systematic studies of HIV-1 resistance mutation in Nepalese population. Although the study shows negative for the M184V resistance mutation, screening of various panels of drug resistance mutations should be performed in recently infected HIV- 1 patients for planning the effective ART strategy.
- keywords: 3tc; aids; amplification; amplified; antiretroviral; art; azt; biotechnology; cviaii; detection; digestion; dna; drug; drug resistance; env; gel; gene; genotypic; high; hiv-1; human; infected; infection; inhibitors; journal; lamivudine; latent; limit; m184v; m184v mutation; minutes; molecular; mutant; mutation; negative; nepal; nepjol.info/index.php/njb; nested; nfv; njb; patients; pcr; positive; present; primers; product; proviral; proviral dna; reaction; research; resistance; resistance mutation; restriction; results; reverse; rfda; rna; samples; sec; sequence; shrestha; size; society; specific; study; table; testing; therapy; transcriptase; treatment; type; v3 dna; variants; virus; vol; wild; ° c
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- njb-274
- author: NJB, Nepal Journal of Biotechnology
- title: Effect of Coconut Water and GA3 Concentrations on in vitro Clonal Propagation of Potato Cultivars from Nepal
- date: 2023-07-31
- words: 5563
- flesch: 67
- summary: 1.16 CW 20 mL/L 9.5cd ± 0.5 5.67a ± 1.15 7bc ± 1 9.33ab ± 0.58 55c ± 6.24 1.02b ± 0.11 Cardinal 0 5.5b ± 0.46 10.03a ± 0.31 2.67a ± 0.58 7.67a ±1.53 0.73b ± 0.10 10.46a ± 1.57 9.84a ± 0.68 0.1 7.93a ± 0.86
- keywords: 200c; abm; agar; agent; basal; biomass; biotechnol; bsn; cardinal; cbm; clarigel; coconut; combination; comparison; composition; concentrations; culture; dev; development; different; dry; g+c; ga3; gellan; gelrite; growth; gum; hairs; himedia; internode; jam; janak; journal; kafle; leaf; length; media; medium; meristem; multiplication; nepal; njb; nodal; nodes; number; performance; plant; plantlets; potato; propagation; rate; regulators; results; root; root length; shoot; size; sprouts; study; survival; tip; tissue; unchanged; values; varieties; variety; water
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- njb-28
- author: Amgai, Resham Babu; Pantha, Sumitra; Bhatta, Madan Raj
- title: Characterization of Nepalese Barley Gene Pool for Leaf Rust Resistance
- date: 2016-12-31
- words: 2914
- flesch: 67
- summary: Mammadov JA, Brooks WS, Griffey CA, Saghai Maroof MA: Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7. Identification of particular rust resistance gene and its incorporation is the only option for the development of barley varieties for leaf rust rust resistance.
- keywords: 5hs; accessions; barley; biotechnology; bonus; characteristics; collection; disease; gene; genetic; genotype; germplasm; gr-25; jau; jc#49; journal; lalitpur; landraces; leaf; leaf rust; local; major; markers; molecular; nb-1003; nepal; nepalese; nepjol.info/index.php/njb; njb; pcr; plant; presence; puccinia; qblr; qtl; research; resistance; rph2; rph7; rust; rust resistance; screening; society; solu; ssr; susceptible; table; uwa; variation; vol
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- njb-29
- author: Maharjan, Mahendra; Mandal, Swati; Madhubala, Rentala
- title: Assessing the Role of Potential Biomarkers in Antimony Susceptible and Resistant Clinical Isolates of L. donovani from India
- date: 2016-12-31
- words: 6676
- flesch: 54
- summary: Comparison of MRPA gene expression in SAG-S versus SAG-R field isolates was done to verify if there is any correlation between MRPA gene expression and SAG susceptibility profile of the clinical isolates. MRPA gene expression in SAG-S versus SAG-R field isolates showed correlation between MRPA gene expression and SAG susceptibility profile of the clinical isolates.
- keywords: abc; ag83; amastigotes; amplification; analysis; antimony; azar; biotechnology; blot; clinical; clinical isolates; control; digested; dna; donovani; drug; earlier; expression; field; figure; fold; gene; hindiii; india; intracellular; isolates; journal; kala; leishmania; leishmaniasis; levels; maharjan; mc4; mc8; mc9; mrpa; nepal; nepjol.info/index.php/njb; njb; nr3a; number; odc; ouellette; patients; pcr; pkdl; present; probe; promastigotes; resistant; resistant isolates; rk1; rmp142; rmp155; rmp240; rmp8; role; sag; sbiii; sensitive; society; southern; specific; strains; study; thiol; time; total; transporter; trypanothione; vol; -gcs
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- njb-3
- author: Khadka, Sundar; Kandel, Samikshya; Pandit, Roshan; Manjhi, Rosham; Dhital, Subhash; Baniya, Jagat Bahadur; Mishra, Shravan Kumar; Mahato, Raj Kumar
- title: Sero-prevalance of Cryptococcal Antigenemia in HIV Positive Individual having CD4 Counts
- date: 2021-12-30
- words: 2550
- flesch: 56
- summary: The study concludes higher prevalence of Cryptococcal antigenemia among HIV infected individuals and recommends Cryptococcal antigen screening to be made mandatory in HIV positive patients having CD4 T cells count below 100/µl. All subjects enrolled in this study were HIV positive patients having CD4 T cells counts less than 100 cells/mm3.
- keywords: agglutination; aids; antigen; antigenemia; assay; cd4; cells; count; crag; cryptococcal; data; deficiency; detection; female; flow; health; higher; hiv; immunocompromised; individuals; infection; journal; laboratory; latex; meningitis; mm3; national; neoformans; nepal; opportunistic; patients; people; positive; prevalence; rate; screening; study; test; total
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- njb-30
- author: Aryal, Sagar; Karki, Gaurab; Pandey, Sunil
- title: Microbial Diversity in Freshwater and Marine Environment
- date: 2015-12-30
- words: 1781
- flesch: 53
- summary: Nominal cell counts of >105 cells per ml in surface sea water [12, 13] predict that the oceans harbor 3.6 X 1029 microbial cells with a total cellular carbon content of 3 X 1017g Freshwater Microbial Diversity Freshwater and marine environments differ in many ways including salinity, average temperature, depth, and nutrient content, but both provide many excellent habitats for microorganisms.
- keywords: bacteria; benjamin; benthic; biotechnology; carbon; cells; cummings; cyanobacteria; depth; diversity; earth; ecology; environment; freshwater; high; life; light; limnetic; marine; meters; microbial; microbiology; microorganisms; nepal; numbers; nutrient; ocean; organic; oxygen; phototrophs; primary; prokaryotic; sea; species; surface; water; zone
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- njb-31
- author: Pant, Narayan Dutt; Sharma, Manisha; Khatiwada, Saroj
- title: Fasciolopsis buski Vomited Out by a Child; The First Case Reported from Nepal
- date: 2015-12-30
- words: 1107
- flesch: 50
- summary: ISSN 2467-9319 (online) CASE STUDY ©NJB, Biotechnology Society of Nepal 66 Nepjol.info/index.php/njb Fasciolopsis buski Vomited Out by a Child; The First Case Reported from Nepal Narayan Dutt Pant1*, Manisha Sharma2, Saroj Khatiwada3 1Department of Microbiology Grande International Hospital, Dhapasi, Kathmandu, Nepal 2Department of Microbiology, Kathmandu medical college, Kathmandu, Nepal 3CIST College, Kathmandu, Nepal Abstract Live adult worms of Fasciolopsis buski are rarely seen in humans except in autopsy. Trop Dis Bull 1994, 91:r126-r150. 4. Mahajan RK, Duggal S, Biswas NK, Duggal N, Hans C: A finding of live Fasciolopsis buski in an ileostomy opening.
- keywords: adult; antihelminthic; biotechnology; buski; case; child; common; diarrhea; fasciolopsis; gastrointestinal; history; humans; india; infection; kathmandu; live; nepal; patient; report; treatment; water; worms
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- njb-32
- author: Kusi, Meena; Shrestha, Kanti; Malla, Rajani
- title: Study on Phytochemical, Antibacterial, Antioxidant and Toxicity Profile of Viscum album Linn Associated with Acacia catechu
- date: 2015-12-30
- words: 4132
- flesch: 54
- summary: [21- 23] Brine Shrimp Lethality Assay: Crude plant extract was prepared in three different concentrations of 1000ppm, 100ppm and 10ppm. Viscum album in Nepal has been used as a medicinal herb since very long.
- keywords: acacia; activity; aerial; album; alkaloids; analysis; antibacterial; antioxidant; assay; associated; atcc; bioactive; biotechnology; brine; cardiac; catechu; compounds; concentration; control; crude; dpph; dry; ec50; extract; ferric; flavonoids; frap; free; glycosides; gram; inhibition; journal; khusi; known; larvae; lc50; linn; medicinal; medicine; methanolic; mistletoe; nepal; nepjol.info/index.php/njb; njb; parts; percentage; phytochemical; plant; positive; presence; radical; reagent; results; saponins; scavenging; science; screening; shrimp; significant; society; standard; study; table; tannins; test; toxicity; use; value; viscum; viscum album; vol
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- njb-33
- author: Awasthi, Tek Raj; Pant, Narayan Dutt; Dahal, Puspa Raj
- title: Prevalence of multidrug resistant bacteria in causing community acquired urinary tract infection among the patients attending outpatient department of Seti Zonal Hospital, Dhangadi, Nepal
- date: 2015-12-30
- words: 2988
- flesch: 51
- summary: ©NJB, Biotechnology Society of Nepal 55 Nepjol.info/index.php/njb Prevalence of Multidrug Resistant Bacteria in Causing Community Acquired Urinary Tract Infection Among the Patients Attending Outpatient Department of Seti Zonal Hospital, Dhangadi, Nepal Tek Raj Awasthi1, Narayan Dutt Pant2*, Puspa Raj Dahal3 1Department of Microbiology, Siddhanath Science Campus, Kanchanpur , Nepal 2Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal 3Department of Microbiology, Trichandra Multiple College, Kathmandu, Nepal Abstract Involvement of multidrug resistant bacteria in causing community acquired infection is very serious public health issue. The main objective of our study was to determine the prevalence of multidrug resistant bacteria in causing community acquired urinary tract infection.
- keywords: aeruginosa; antibiotics; antimicrobial; aureus; bacteria; biotechnology; coli; community; department; different; escherichia; gram; growth; health; hospital; infection; involvement; isolates; kathmandu; mdr; microbiology; multidrug; negative; nepal; patients; positive; prevalence; proteus; region; resistant; samples; shrestha; significant; staphylococcus; study; susceptibility; susceptible; test; total; tract; urinary; urine; uti
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- njb-34
- author: Shrestha, Pritish; Bista, Manisha; Sharma, Prativa; Shrestha, Shristi; Lamichhane, Basanta; Adhikari, Sandeep; Pandey, Binayak Raj; Shrestha, Bhupal Govinda
- title: Phytochemical screening, antimicrobial activity and cytotoxicity of Nepalese medicinal plants Swertia chirayita and Dendrobium amoenum
- date: 2015-12-30
- words: 3196
- flesch: 58
- summary: A, B, C, D, E and F represents ZOI of different plant extract against S. paratyphi, E. coli, K. pneumoniae, S. typhi, P. aerugenosa and S. aureus respectively. Plant extract showed the presence of antioxidants like alkaloid, terpenoids, flavonoids, tannin, glycosides.
- keywords: acetate; activity; amoenum; antimicrobial; biotechnology; brine; chiraito; chirayita; cold; concentration; cytotoxicity; daman; death; dendrobium; dhunche; different; equation; ethyl; extract; extraction; figure; graph; inhibition; journal; lc50; medicinal; medium; methanol; nepal; nepjol.info/index.php/njb; njb; percentage; phytochemical; plants; ppm; research; room; sample; science; screening; shrestha; shrimp; society; solution; species; swertia; temperature; test; value; vol; warm; yield; zoi; zone
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- njb-35
- author: Joshi, Bal K; Louws, Frank J; Yenco, G Craig; Sosinski, Byron R; Arellano, Consuelo; Panthee, Dilip R
- title: Molecular Markers for Septoria Leaf Spot (Septoria lycopersicii Speg.) Resistance in Tomato (Solanum lycopersicum L.)
- date: 2015-12-30
- words: 4755
- flesch: 63
- summary: We studied the inheritance of SLS resistance and identified molecular markers linked to SLS resistance using bulked segregant analysis (BSA) in a segregating F2 population. Subject to verification in independent populations, these markers may be useful for MAS of SLS resistance in tomato.
- keywords: 839; analysis; area; band; biotechnology; blight; breeding; bsa; bulk; carolina; disease; dna; dominant; early; et al; figure; gene; individuals; jhoshi; journal; leaf; lines; lycopersici; markers; mas; molecular; nepal; nepjol.info/index.php/njb; njb; north; parent; pcr; plants; polymorphic; population; primers; rapd; reaction; resistance; score; segregant; septoria; single; size; sls; society; spot; study; susceptible; symptoms; tomato; total; university; vol
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- njb-36
- author: Amgai, Resham Babu; Niroula, Raj Kumar; Pantha, Sumitra; Hamal, Shreya Singh; Tamang, Bishal Gole; Sah, Bindeshwar Prasad; Bhatta, Madan Raj
- title: Marker Assisted Screening of Nepalese rice for bacterial leaf blight (BLB) resistance
- date: 2015-12-30
- words: 1845
- flesch: 61
- summary: BLB resistance rice varieties are highly demanded worldwide. We have detected BLB resistance gene Xa-10 on five accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions.
- keywords: absence; accessions; agriculture; amgai; bacterial; biotechnology; blb; blight; breeding; check; council; different; disease; dna; gene; genetic; journal; landraces; leaf; marker; molecular; nepal; nepalese; nepjol.info/index.php/njb; njb; pcr; plant; presence; research; resistance; rice; screening; society; table; varieties; variety; vol; xa-5
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- njb-37
- author: Thapa, Sandeep; Shrestha, Rajani; Tirewal, Anjali; Sharma, Arjun; K.C., Yuvraj
- title: Isolation of yeast from soil and different food samples and its characterization based on fermentation
- date: 2015-12-30
- words: 3097
- flesch: 54
- summary: The authors are also thankful to Pinnacle Academy, Lalitpur, Nepal and Mr. Sushil Khanal from Central Department of Biotechnology, Trubhuvan University, Nepal for providing us Yeasts samples. Simple staining was performed to elucidate the morphology and arrangement of yeast cells and budding.
- keywords: absorbance; alcoholic; bioethanol; biotechnology; broth; carbon; cells; cerevisiae; characteristics; color; concentration; culture; day; dichromate; different; ethanol; extraction; fermentation; glucose; growth; high; isolated; journal; media; method; microbiol; mucoid; nepal; nepjol.info/index.php/njb; njb; oval; oxidation; peptone; phase; plate; production; purple; sample; smell; smooth; society; solution; solvent; source; spot; standard; study; table; thapa; vol; xylose; yeast
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- njb-38
- author: Maharjan, Mahendra; Madhubala, Rentala
- title: Heat shock protein 70 (HSP70) expression in antimony susceptible/resistant clinical isolates of Leishmania donovani
- date: 2015-12-30
- words: 4441
- flesch: 57
- summary: HSP70 gene copy number, gene expression and HSP70 protein expression was found uniform in both antimony sensitive and resistant clinical isolates. A time dependent increase of HSP70 protein expression in L. donovani field isolates was observed.
- keywords: ag83; analysis; antibody; antimony; biotechnology; blot; cells; clinical; copy; dna; donovani; drug; expression; field; figure; fold; gel; gene; genomic; heat; hindiii; hours; hsp70; hsp70 gene; hsp70 protein; hybridization; induction; infantum; isolates; journal; lane; leishmania; leishmaniasis; level; maharjan; mice; molecular; mutants; nepal; nepjol.info/index.php/njb; njb; northern; number; pcr; pfge; probe; promastigotes; protein; purification; recombinant; resistant; sag; sbiii; sds; sensitive; sequence; shock; single; society; southern; stress; tarentolae; time; vector; vol; western
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- njb-39
- author: Jha, Bimlesh Kumar; Gupta, Birendra Prasad; Maharjan, Prashanna; Kakshapati, Somila; Munankarmi, Nabin Narayan
- title: Effect of lyophilization on infectivity and viral load of Adenovirus
- date: 2015-12-30
- words: 4375
- flesch: 53
- summary: Adenovirus PCR was done in virus infected cell line where a fragment of 161bp was amplified from hexon gene of AdV genome and visualized by 2% agarose gel electrophoresis (Figure 1). To determine the effect of lyophilization on virus titer the lyophilized virus after revival was titrated in Hep-2 cell line and virus titer was determined by TCID50 and PFU following the same procedure as described earlier for the stock virus.
- keywords: adenovirus; adv; biotechnology; buffer; cell; centrifuge; column; culture; difference; dna; drying; effect; figure; freeze; ice; infected; infectivity; insert; jha; journal; ligation; load; lyophilization; lyophilized; methods; min; mini; months; nepal; nepjol.info/index.php/njb; njb; pcr; pellet; rapid; reaction; real; revived; significant; society; sterile; stock; storage; study; supernatant; tcid50; temperature; time; tissue; titer; tube; viral; viral load; virus; viruses; vol; water; white
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- njb-4
- author: Koirala, Niranjan; Khanal, Sareeta; Chaudhary, Sujan; Gautam, Sagar; Sah, Shiv Nandan; Subba, Prince; Marraiki, Najat; Batiha, Gaber El-Saber
- title: Potential surface active agent production using very low grade and cheap substrate by Bacillus subtilis as microbial cell factory
- date: 2021-12-30
- words: 5110
- flesch: 52
- summary: The emulsification activity of cell free broth extracted from soya cake This study recorded the emulsification activity was observed highest in mustard oil with the cell free extract from mustard oil cake whereas engine oil showed the lowest (Figure. 1). Emulsification activity in mustard oil from extract from mustard oil cake was found to be 1.13, 0.99, 0.81, 0.95 and 0.76 at the time interval of 1, 2, 3, 4 and 5 hour respectively.
- keywords: ability; active; activity; applications; applied; bacillus; bacterial; bio; biochemical; biosurfactants; biotechnology; broth; bsn; cake; cell; chemical; cost; crude; day; dec.9; different; e24; emulsification; emulsification index; engine; engine oil; environmental; extract; figure; free; higher; highest; index; industrial; journal; kerosene; koirala; lowest; medium; microbial; microbiology; mustard; mustard cake; mustard oil; nepal; njb; oil; oil cake; photograph; positive; potential; production; properties; range; research; result; soil; soya; soya cake; strain; study; substrate; subtilis; sunflower; sunflower oil; surface; surfactants; table; tension; test; time; university; value; water; whey
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- njb-40
- author: Sah, Surendra Kumar; Khatiwada, Saroj; Chaudhary, Deepak; Jha, Chandra Bhushan; Bhattacharya, Soumya
- title: Doxorubicin induced histomorphometric changes in testes of albino rat
- date: 2015-12-30
- words: 3045
- flesch: 51
- summary: We also observed progressive decrease in testis weight from short term to long term experimental group. As compared to short term experimental group, midterm experimental group and long term experimental group have decreased diameter and cross-sectional area of the seminiferous tubules, which may be due to more effect of the drugs on exposure to long time.
- keywords: albino; animals; area; biotechnology; body; cells; changes; control; cross; damage; diameter; difference; dose; doxorubicin; effects; epithelium; experimental; experimental group; group; health; journal; long; long term; male; midterm; nepal; njb; normal; p<0.001; present; rats; right; sectional; seminiferous; seminiferous tubules; short; short term; significant; single; study; term; term experimental; testicular; testis; testis weight; tubules; volume; week; weight
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- njb-41
- author: Shrestha, Geeta Rajbhandari; Mridha, Amin Udhin
- title: Detection and Quantitation of Aflatoxin for the Diagnosis of Aspergillus flavus
- date: 2015-12-30
- words: 2615
- flesch: 55
- summary: The different strains of A. flavus producing aflatoxins were screened on Aflatoxin Producing Medium (APM) as described by Donald et al. (1981) Aflatoxins production is low during early stages of growth that increases with the decline of growth and the maximum production was recorded after the stationary phase of growth, which soon decline in its production (Table 1 and Figure 1).
- keywords: aflatoxins; aftb1; aspergillus; biotechnology; cancer; carcinoma; cause; contamination; days; detection; different; diseases; effect; elisa; exposure; factors; figure; flavus; food; groopman; growth; health; hepatocellular; highest; human; incubation; level; media; medium; method; mycotoxins; nepal; periods; production; samples; significant; storage; study; sucrose; temperatures; test; tlc
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- njb-42
- author: Upreti, Deepak; Sapkota, Naresh Prasad; Aryal, Bibek; Shakya, Sangita
- title: Accumulation of Poly-hydroxy-butyric Acid (PHB) by Bacillus Strain Isolated from Paddy Field of Kathmandu University Premises
- date: 2015-12-30
- words: 2165
- flesch: 52
- summary: Screening for PHB production To test the production of PHB, seed culture of isolated colonies were grown in 10 ml Nutrient broth and incubated for 24 hours at 30 °C. Results Of the fifteen samples evaluated for PHB production, DNB-6 isolate accumulated the maximum PHB (31.91%) of dry cell weight.
- keywords: accumulation; acid; bacillus; bacteria; biodegradable; biotechnology; cell; chloroform; concentration; curve; deficient; different; dnb-6; figure; glucose; growth; hours; intensity; isolate; maximum; media; nepal; nitrogen; nutrient; phase; phb; poly; polymer; production; soil; strain; time; upreti; yield
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- njb-43
- author: Rosyara, Umesh R; Joshi, Bal K
- title: Association Mapping for Improvement of Quantitative Traits in Plant Breeding Populations
- date: 2022-07-11
- words: 7281
- flesch: 53
- summary: In the present review, we discuss different mapping populations, approaches, prospects and limitations of using association mapping in plant breeding populations. Jan. 2012, Vol. 2, No. 1: 72 – 89 Biotechnology Society of Nepal (BSN), All rights reserved 72 REVIEW ARTICLE Association Mapping for Improvement of Quantitative Traits in Plant Breeding Populations Umesh R. Rosyara1 and Bal K. Joshi2 1 South Dakota State University, Plant Science Department, Brookings, South Dakota, USA 2 Biotechnology Unit, NARC, Khumaltar, PO Box 1135 Kathmandu, Nepal *Correspondence Author recent address: joshibalak@yahoo.com ABSTRACT DNA-based molecular markers have been extensively utilized for mapping of genes and quantitative trait loci (QTL) of interest based on linkage analysis in mapping populations.
- keywords: 2000; 2004; acad; advanced; allele; analysis; approach; association; association mapping; biotechnology; breeding; bsn; candidate; case; collections; control; core; crop; cross; data; decay; different; disequilibrium; diversity; effects; elite; expected; extended; extreme; families; family; fine; generations; genet; genetic; genome; genomic; genotype; genotyping; germplasm; high; hum; improvement; individuals; information; jan; journal; lines; linkage; loci; low; maize; mapping; markers; method; natl; nepal; number; parents; phenotypic; plant; polymorphism; populations; power; proc; progeny; programs; qtl; quantitative; recent; recombination; related; results; rights; samples; sci; selection; significant; single; size; society; structure; studies; study; synthetic; test; time; trait; transmission; unlinked; usa; use; variation; vol
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- njb-44
- author: Deswal, Sumit
- title: Models of T cell antigen receptor activation: the puzzle still remained
- date: 2022-07-11
- words: 3650
- flesch: 52
- summary: Although the downstream signaling mechanisms of T cell activation have been explored to quite detailed extent, the basic mechanism of how ligand binding to extracellular domains of TCRαβ leads to downstream signaling remained unclear. E-mail: deswal@immunbio.mpg.de, Tel.: +49-761-5108-314, FAX: +49-761-5108-423 Abstract T cell antigen receptor (TCR) is a protein-complex expressed on all T cells of the immune system and is responsible for the activation of T cells when infectious agent is presented by an antigen presenting cell (APC) in the form of peptides bound to the major histocompatibility complex (pMHC).
- keywords: activation; antigen; binding; biotechnology; bsn; cd3; cd4; cd45; cd8; cell; change; class; clustering; complex; conformational; cytoplasmic; geometry; heterodimer; homodimer; immunol; itams; jan; journal; kinase; kinetic; lck; ligand; lipid; membrane; model; molecules; nepal; observations; phosphatase; phosphorylation; pmhc; protein; raft; receptor; rights; sci; segregation; signaling; society; structure; t cell; tcr; vol
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- njb-45
- author: Singh, Dipali; Tripathi, Anushree; Kumar, Gautam
- title: An Overview of Computational Approaches in Structure Based Drug Design
- date: 2022-07-11
- words: 3147
- flesch: 52
- summary: This review summarizes the methods of structure based drug design, usage of related softwares and a case study that explores to find a suitable drug (lead) molecule for the mutated state of H-Ras protein in order to prevent complex formation with Raf protein. Keywords: computer aided drug design, structure based drug design, Ras-protein Introduction Traditionally, new drugs were generated from plants and other natural products through accidental observations and discoveries.
- keywords: acid; active; approaches; binding; biotechnology; bsn; cancer; candidate; cell; compounds; computational; computer; database; dec; design; discovery; docking; drug; drug design; energy; enzyme; field; fit; fragment; functional; grid; growth; hydroxyurea; inhibitors; journal; lead; ligand; methods; molecular; molecule; mutated; nepal; new; novo; p21; potential; process; protein; ras; review; rights; sbdd; scoring; site; small; society; state; structure; target; vol
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- njb-46
- author: Patel, Hardik A; Patel, Rajesh K; Khristi, Sunil M; Parikh, Kruti; Rajendran, Geetha
- title: Isolation and Characterization of Bacterial Endophytes from Lycopersicon esculentum Plant and their Plant Growth Promoting Characteristics
- date: 2022-07-11
- words: 5540
- flesch: 57
- summary: PGPR have been reported to directly enhance plant growth by the production of plant growth regulators, and improvements in plant nutrient uptake [12,16] or indirectly by the production of metabolites like antibiotics, siderophores etc that decrease the growth of phytopathogens [12]. Four of the strains among the 18 showed maximum positive results of plant growth promoting regulators (PGPR) test and among them best probable isolate was selected and thus its 16SrDNA was amplified and sequenced.
- keywords: 16s; acetic; acid; activity; agar; amplified; assay; bacilli; bacteria; biotechnology; bsn; cell; characteristics; chromium; clearance; cocco; culture; different; endophytic; entire; esculentum; flat; gram; growth; growth hr; host; hr10; hr13; hr3; hr4; hr7; hr8; hr9; hrs; iaa; indole; irregular; isolates; jan; journal; lycopersicon; maximum; medium; microbiol; min; morphological; motile; negative; nepal; nutrient; opaque; organic; pectinase; pgpr; phosphate; pigmentation; plant; plant growth; plate; positive; presence; production; promotion; rdna; results; rights; root; rough; round; sequence; siderophore; small; smooth; society; soil; sterile; strains; study; table; tolerance; transparent; vol; yellow; zone
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- njb-47
- author: Latha, Parvatham Madhu; Chanakya, Pallem; Srikanth, Manipati
- title: Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake
- date: 2022-07-11
- words: 2912
- flesch: 48
- summary: [11]. Concentration of Lovastatin (mg/g) = Concentration of Lovastatin (mg/ml) x Dilution factor (DF) Amount of substrate taken (g) Results and Discussion Selection of solid substrates in SSF for lovastatin production: Solid substrates employed in SSF processes are heterogeneous natural raw-materials which are generally insoluble in water and play a dual role-supply of nutrients to the microbial culture growing and anchorage for the growing cells. 2: Effect of moisture content on lovastatin production Effect of Inoculum volume Fermentation was carried out with different inoculum volumes (0.5-4ml) of five day old A. fischeri culture for a period of 144h to study its effect on lovastatin production.
- keywords: aspergillus; biotechnology; bsn; cake; coconut; content; culture; dry; effect; fermentation; fig; fischeri; growth; initial; inoculum; jan; journal; lovastatin; maximum; microbial; moisture; nepal; oil; parameters; present; process; production; rights; society; solid; sources; ssf; state; study; substrate; temperature; time; vol; volume; yield
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- njb-48
- author: Joshi, Bal K; Bimb, Hari P; Kansakar, David; Ghimire, Ekta
- title: Genetic Relationship among Nepalese Rice Landraces and Cultivars based on RAPD Markers
- date: 2022-07-11
- words: 3173
- flesch: 72
- summary: Key words: Genetic distance, rice, RAPD Introduction Nepal is rich in rice genetic resources The three blast resistance genes pyramided rice genotype, A57-115-8 was genetically near with Anpjutte, Tauli and Thapachini.
- keywords: a57; abd; analysis; bands; biotechnology; blast; breeding; bsn; cluster; collection; cultivars; dhan; diversity; dna; genetic; genome; genotypes; hardinath; highest; information; ir-24; jan; jarneli; joshi; journal; kali; khumaltar; lalshar; landrace; line; mansara; markers; min; mugu; nepal; nr10276; nr10414; nrrp; number; plant; polymorphism; pranpyuri; primers; quadrant; rapd; rice; rights; sample; similarity; society; study; table; tag; varieties; vol
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- njb-49
- author: Dulal, Santosh; Ban, Bhupal; Yang, Gi Hyoek; Jung, Hyun Ho
- title: Cloning, Expression, Purification, and Characterization of Clostridium botulinum Neurotoxin Serotype F Domains
- date: 2022-07-11
- words: 5985
- flesch: 54
- summary: Jan. 2012, Vol. 2, No. 1: 1 – 15 Biotechnology Society of Nepal (BSN), All rights reserved 10 strong affinity towards F-HN and native type F toxin (Figs. 5b and 6b). Conclusion This paper describes molecular and immunological studies on C. botulinum neurotoxin type F domains.
- keywords: affinity; amplified; analysis; antibodies; antibody; binding; biotechnology; bont; botulinum; botulism; bsn; catalytic; cells; chain; chemicals; chromatography; cloning; coli; concentrations; dna; domains; elisa; expression; fig; free; fusion; gst; hcc; heavy; high; immunoreactivity; inc; indirect; induction; iptg; jan; journal; kda; lane; langeland; light; mice; microbiol; min; mouse; native; nepal; neurotoxin; neutralizing; page; pbs; pcr; pgex-4t-1; plasmids; plates; polyclonal; proteins; purification; purified; receptor; recombinant; restriction; rights; ril; sds; sequence; serotype; serum; society; soluble; specific; substrate; subunit; terminal; thrombin; translocation; type; usa; vaccine; vector; vol; western
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- njb-5
- author: Maharjan, Rasna; Thapa, Prakash; Khadayat, Karan; Kalauni, Surya Kant
- title: Phytochemical Analysis and α-Amylase Inhibitory Activity of Young and Mature Leaves of Cinnamomum tamala
- date: 2021-12-30
- words: 5161
- flesch: 58
- summary: C. tamala leaves exhibited significant biological properties with scientific validation including antioxidant [16,17], antimicrobial [18,19,20], anti-diabetic [21,22,23], anticancer [24], and anti-inflammatory Methanolic young leaves extract showed α-amylase inhibition with IC50 value 224.6 ± 2.76 μg/mL as compared to acarbose with IC50 value 5.93 ± 0.14 μg/mL. The result suggests that young leaves of C. tamala had anti-diabetic activity so further work should be carried out.
- keywords: 2):14; absorbance; acarbose; acid; activity; amylase; analysis; antioxidant; available; biotechnol; bsn; c. tamala; carbohydrates; cinnamomum; compounds; concentration; content; control; dec.9; department; diabetes; diabetic; different; diseases; eem; eey; enzyme; extract; extraction; flavonoid; food; gae; health; higher; ic50; inhibition; inhibitory; insulin; internet; leaf; leaves; maharjan; mature; mature leaves; mean; medicinal; mem; methanolic; mey; nepal; nitrophenyl; njb; pancreatic; percentage; phenolic; phytochemical; plants; potential; present; properties; quercetin; research; sample; sci; screening; sem; sep; standard; study; substrate; table; tamala; temperature; tfc; therapeutic; total; tpc; tribhuvan; type; university; value; vitro; wang; yield; young; young leaves
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- njb-50
- author: Dhakal, Dipesh
- title: The Future Science
- date: 2022-07-11
- words: 435
- flesch: 34
- summary: Hence, the future science should be BE NATURAL WITH SUSTAINABILITY! For that the idea from the developed community should be mingled with the natural resources available in hotspots patches of globe, to create a utopian era.
- keywords: current; globe; life; natural; resources; scientific; system
- versions: original; plain text
- njb-51
- author: Dhakal, Dipesh
- title: Race for Excellence
- date: 2022-07-11
- words: 425
- flesch: 48
- summary: Utilizing the science of biology, chemistry, physics, engineering, and information technology to develop products and services of great promise is the threshold of current scientific research. The fundamental backbone of scientific clues and technological intervention relies on course and discourse of the natural attributes within the nature itself; taking into consideration of the physical, chemical, biological and computational prospect.
- keywords: human; implications; life; nature; race; science; scientific; technology
- versions: original; plain text
- njb-52
- author: Adhikari, Raju; Adhikari, Benu
- title: Biotechnology Growth Partnership: A Potential Flagship Program in S&T
- date: 2022-07-11
- words: 2280
- flesch: 44
- summary: Nepal currently trades about 36 different types of products derived from herbal plants and the total revenue earned is reported to be approximately 12 million NRs (US $240,000). The clinical trials and manufacturing of products could be carried out in Nepal which will help develop necessary infrastructure for the production of such value added products and clinical trial facilities in the long run on low cost.
- keywords: acid; adhikari; areas; australia; biotechnology; cheese; cordyceps; countries; country; government; high; medicinal; mycelia; nepal; overseas; partnership; plants; potential; products; project; raw; research; s&t; scientific; sinensis; value; work; yak
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- njb-53
- author: Ray, Bishnu Pada; Hassan, Lutful; Sarker, Smreeti Kana
- title: In vitro cultivation and regeneration of Solanum melongena (L.) using stem, root and leaf explants
- date: 2022-07-11
- words: 2581
- flesch: 62
- summary: Key words: Regeneration, BAP, NAA. Correspondence Author: E‐mail: bpray2003@yahoo.com Introduction Brinjal (Solanum melongena L.), belongs to the family Solanaceae, is one of the most popular, palatable and nutritious vegetable crop in Bangladesh. Abstract The treatment combinations was BAP (0, 2.0, 3.0 and 4.0 mg/L) and NAA (0, 0.1, 0.5, and 1.0 mg/L).
- keywords: abcde; bap; biotechnology; callus; combinations; days; defg; explants; induction; klmno; leaf; medium; naa; nepal; regeneration; stem
- versions: original; plain text
- njb-54
- author: Ammann, Klaus
- title: Molecular differences between GM- and non-GM crops over-estimated?
- date: 2022-07-11
- words: 10606
- flesch: 29
- summary: Nature, 356, 6364, pp 1‐2 http://dx.doi.org/10.1038/356001b0 AND http:// www.botanischergarten.ch/Regulation/Anonymous‐US‐ Regulation‐Nature‐1992.pdf Arber, W. (1994) MOLECULAR EVOLUTION: COMPARISON OF NATURAL AND ENGINEERED GENETIC VARIATIONS. Plant Cell Reports, 18, 10, pp 858‐862 http://dx.doi.org/10.1007/s002990050674 AND http:// www.botanischergarten.ch/Mutations/Barnabas‐ Colchicine‐Genome‐Doubling‐1999.pdf Barros, E. (2010) Molecular Profiling Techniques as Tools to Detect Potential Unintended Effects in Genetically Engineered Maize (revised title), Molecular Profiling Techniques Detect Unintended Effects in Genetically Engineered Maize (old title).
- keywords: academy; action; america; ammann; analysis; arber; assessment; biosafety; biotechnology; breeding; bsn; case; changes; chromosome; comparison; conclusions; conventional; crops; data; differences; different; dna; dynamics; early; effects; elements; engineering; environmental; evolution; evolutionary; expression; fedoroff; fig; food; gene; genetic; genome; genomic; horizontal; http://; impact; important; isi>://wos; jan; journal; lack; level; lines; locus; maize; mcclintock; methods; molecular; mutation; national; natural; nature; nepal; new; organic; paper; plants; polyploids; possible; proceedings; process; processes; recent; regulation; regulatory; research; rights; risk; sciences; scientific; sequences; similar; society; soltis; species; states; system; time; transcriptome; transfer; transgenesis; transgenic; transposition; united; van; vol; wheat
- versions: original; plain text
- njb-55
- author: Chandan, S; Umesha, S; Bhure, SK; Haraprasad, N; Chandrashekar, S
- title: Development of PCR assay for targeting partial lipL21 and lipL41 gene of leptospira
- date: 2022-07-11
- words: 4502
- flesch: 52
- summary: Primer degradation was suspected and the PCR reactions at the optimized conditions were repeated with fresh primer set P30/31. PCR reactions were again carried out on nine serovars at MgCl2 Results and Discussion Specificity of Leptospira spp. Primers. The following conditions were chosen: PCR was performed in 25 ml reaction volume containing 10x Taq buffer with KCl (100mM Tris HCl, 50mM KCl, 0.8% Nonidet P40), 1.5mM MgCl2, 2.5mM of each dNTPs, 2U of Taq polymerase, 25pmoles of each primer and 0.25ml of DNA (40ng/ml) extract from L. icterohaemorrhagiae.
- keywords: agarose; annealing; assay; biotechnology; buffer; detection; dna; enzyme; fig; figure; gel; gene; jan; lane; leptospira; leptospirosis; lipl41; method; min; nepal; p28/29; p30/31; pathogenic; pcr; primer; product; reaction; restriction; run; sequence; serovars; species; specificity; standardized; table; temperature; template
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- njb-56
- author: Shrestha, Raunak; Joshi, Rubin Narayan; Joshi, Kriti; Poudel, Bal Hari; Shrestha, Bhupal Govinda
- title: Analysis of KatG Ser315Thr Mutation in Multidrug Resistant Mycobacterium tuberculosis and SLC11A1 Polymorphism in Multidrug Resistance Tuberculosis in Central Development Region of Nepal Using PCR-RFLP Technique: A Pilot Study
- date: 2022-07-11
- words: 3600
- flesch: 48
- summary: Samples 021, 022, and 023 has a band size of 153 bp indicating no mutation at 315th codon position where as sample 020 and 024 has a band sized 132 bp indicating SeràThr mutation at 315th codon position of KatG gene. This fact remained as a challenge SampleID MDR‐TB INT4 PCR amplicon size (bp) ApaI digested product of INT4 (bp) G/C Polymorphism in INT4 SLC‐001 POSITIVE 514 514 NEGATIVE SLC‐002 POSITIVE 514 514 NEGATIVE SLC‐003 POSITIVE 514 514 NEGATIVE SLC‐004 POSITIVE 514 514 NEGATIVE SLC‐005 POSITIVE 514 514 NEGATIVE SLC‐006 POSITIVE 514 514 NEGATIVE SLC‐007 POSITIVE 514 514 NEGATIVE SLC‐008 POSITIVE 514 514 NEGATIVE SLC‐009 POSITIVE 514 514 NEGATIVE SLC‐010 POSITIVE 514 514 NEGATIVE SLC‐011 POSITIVE 514 514 NEGATIVE SLC‐012 POSITIVE 514 514 NEGATIVE SLC‐013 POSITIVE 514 514 NEGATIVE SLC‐014 POSITIVE 514 514 NEGATIVE SLC‐015 POSITIVE 514 514 NEGATIVE SLC‐016 POSITIVE 514 514 NEGATIVE SLC‐017 POSITIVE 514 514 NEGATIVE SLC‐018 POSITIVE 514 514 NEGATIVE SLC‐019 POSITIVE 514 514 NEGATIVE SLC‐020 POSITIVE 514 514 NEGATIVE SLC‐021 POSITIVE 514 514 NEGATIVE SLC‐022 POSITIVE 514 514 NEGATIVE SLC‐023 POSITIVE
- keywords: agarose; biotechnology; cases; control; dna; drug; figure; fragment; gel; gene; genomic; inh; int4; katg; mdr‐tb; mtb; mutation; negative; nepal; pcr; polymorphisms; positive; region; resistance; restriction; samples; ser315thr; slc11a1; study; tuberculosis
- versions: original; plain text
- njb-57
- author: Mathew, Shiju; Thomas, George; Ahmad, Tufail
- title: An Evaluation of the Fungi Isolated from Sub-epidermal Region of Post-harvested Stored Wheat Grains
- date: 2022-07-11
- words: 2810
- flesch: 59
- summary: Fungal activity can cause undesirable effects in grains including discolouration, contribute to heating and losses in nutritional value, produce off‐odours, losses in germinability, deterioration in baking and milling quality, and can result in contamination by mycotoxins (Hocking, 2003; Magan et al., 2003). Jan. 2011, Vol. 1, No. 1 : 9‐13 13 Biotechnology Society of Nepal (BSN), All rights reserved Detection techniques for stored‐product insects in grain.
- keywords: agar; allahabad; alternaria; aspergillus; biotechnology; different; epidermis; extract; factors; food; fungal; fungi; grains; harvest; india; losses; microorganisms; mycelium; nepal; quality; samples; solution; species; storage; studies; sub‐epidermal; university; water; wheat
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- njb-58
- author: Joshi, Bal K; Panthee, Dilip R
- title: Information Theory and Multivariate Techniques for Analyzing DNA Sequence Data: An Example from Tomato Genes
- date: 2022-07-11
- words: 4699
- flesch: 63
- summary: Main objective was use of information theory and multivariate techniques to understand diversity among genes and relate the sequence complexity with phenotypes. Sequences similarity among genes was observed within a family.
- keywords: amino; analysis; atchley; biotechnology; cluster; complexity; component; crop; data; disease; diversity; dna; entropy; evolution; fruit; genes; genetic; information; journal; molecular; multivariate; mutual; nepal; potato; principal; protein; pseudomonas; quality; resistance; sequences; species; theory; tomato; value; variation
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- njb-62
- author: Daniel, Imaobong E.; Akpan, Ekemini I.; Utam, Edidiong C.
- title: Phytochemical Evaluation, Antioxidant and Antimicrobial Activities of Various Extracts from Leaves and Stems of Bryophyllum pinnatum
- date: 2020-07-31
- words: 8255
- flesch: 56
- summary: Magnesium strip and HCl were mixed with plant extracts. Inhibition of Listeria monocytogenes and Aeromonas hydrophila by plant extracts in refrigerated cooked beef.
- keywords: ability; absorbance; acetate; acetate extracts; acetate leaf; acetate stem; acid; activities; activity; aeruginosa; agar; albican; alkaloids; anthocyanins; antimicrobial; antimicrobial activity; antioxidant; antioxidant activity; assay; aureus; bacteria; bioactive; biotechnol; bryophyllum; bryophyllum pinnatum; bsn; candida; capacity; chemistry; coli; color; compounds; concentrated; concentration; crude; daniel; determination; determined; different; different extracts; distilled; dpph; escherichia; ethyl; ethyl acetate; extracts; ferric; figure; flavonoids; food; frap; free; highest; human; ic50; inhibition; isolates; jan; journal; leaf; leaf extracts; leaves; medicinal; methanol; methanol ethyl; methanol extracts; methanol leaf; methanol stem; method; mixture; ml methanol; nepal; niger; nigeria; njb; organisms; parts; phenolic; phytochemical; pinnatum; plant; plant extracts; polyphenols; power; presence; present; present present; properties; radical; reagent; research; resistant; results; salmonella; saponins; scavenging; screening; solution; spp; standard; stem; stem extracts; study; table; tannins; test; total; vitro; water
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- njb-64
- author: Joshi, Bal Krishna; Joshi, Darbin; Ghimire, Surya Kanta
- title: Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers
- date: 2020-07-31
- words: 5501
- flesch: 64
- summary: Knowledge on genetic diversity is important to manage and use of finger millet landraces and genotypes properly. We have used finger millet landraces from just few farmers of two districts.
- keywords: accessions; agricultural; amplification; analysis; applied; assessment; bands; biotechnol; breeding; bsn; buffer; cluster; conservation; coracana; correlation; crop; data; dendrogram; development; dhading; different; distance; diversity; dna; eleusine; et al; figure; finger; finger millet; gaertn; gel; genetic; genetic diversity; genotypes; germplasm; group; highest; information; joshi; joshi et; journal; jun; kalo; kaski; landraces; lowest; major; markers; mean; millet; millet landraces; min; molecular; nei; nepal; nepal j; njb; number; pic; plant; polymorphic; population; primers; principal; profiles; purbeli; rapd; rapd markers; research; resources; scoring; ssr; ssr markers; ssr-06; study; table; ugep-10; ugep-53; upgma; value
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- njb-66
- author: Bhandari, Ganesh; Pokhrel, Basant; Oli, Yogesh; Katuwal, Archana; Bhandari, Netra Lal
- title: Screening of Methicillin Resistant Staphylococcus aureus (MRSA) from Wounds in Pediatric Patients Visiting Tertiary Care in Hospital
- date: 2019-12-29
- words: 4020
- flesch: 55
- summary: 278 Sensitive % Resistant % Amikacin Ciprofloxacin Cotrimoxazole Cefoxitin Erythromycin Cloxacillin Cefotaxime Penicillin Ofloxacin Chloramphenicol Gentamicin 236 100 125 176 166 180 236 28 116 255 264 84.89 35.97 44.96 63.30 59.7 64.74 84.89 10.08 41.72 91.72 94.96 42 178 153 102 112 98 42 250 162 23 14 15.10 64.02 55.04 36.69 40.28 35.25 15.10 89.92 58.27 8.27 5.03 Table 7. Resistant % Sensitive %
- keywords: agar; age; antibiotic; aureus; bacterial; bhandari; biotechnology; cefoxitin; children; chloramphenicol; clinical; culture; department; different; distribution; female; figure; gender; gentamicin; growth; health; hospital; infections; isolates; journal; laboratory; male; methicillin; microbiology; mrsa; mssa; nepal; nepjol.info/index.php/njb; njb; number; outpatients; patients; pattern; penicillin; positive; prevalence; pus; resistant; s. aureus; samples; sensitivity; society; staphylococcus; staphylococcus aureus; study; susceptibility; table; total; university; vol
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- njb-67
- author: Kumari, Rinki
- title: Potential of Ayurvedic Drugs in Differentiating Neuronal Stem Cells from Human Breast Milk: A Review
- date: 2019-12-29
- words: 6480
- flesch: 50
- summary: Recently, Stem cell therapy has revolutionized excellent clinical therapy with the potential of stem cells to differentiate into various cell types and it may help to replace different cell lines of an organism. Stem cells, able to construct every type of human tissues or organ and due to unlimited self-renewal to produce progeny exactly the same as the originating cell with the extremely regulated mode.
- keywords: 2015; able; acid; active; activity; adult; alternative; alzheimer; angelica; applicable; application; ayurveda; ayurvedic; biotechnology; blood; body; bone; brain; breast; breast milk; cell therapy; cells; chinese; clinical; complement; components; development; differentiation; disease; disorders; drugs; effect; embryonic; et al; evidence; expression; extract; factor; family; figure; formulation; ginkgo; good; growth; hbm; health; herbal; high; hmscs; human; human breast; important; indian; induced; infants; int; issues; journal; kim; kumari; like; lineage; literature; mammary; marker; marrow; mechanisms; medhya; medicinal; medicine; mesenchymal; milk; molecules; nepal; nepjol.info/index.php/njb; nestin; neural; neurodegenerative; neurogenesis; neurogenic; neuronal; neurons; njb; non; organ; papaya; placenta; plant; polyherbal; positive; potential; presence; process; progenitor; proliferation; properties; rasayana; regenerative; repair; research; review; role; root; scientific; sinensis; single; society; source; specific; stem; stem cells; stimulant; studies; study; system; therapeutic; therapy; tissue; traditional; treatment; type; use; vol; zhang
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- njb-68
- author: Thapa, Rojina; Thapa, Rambika; Mahat, Sunita; Thapa, Rupak
- title: Polyphenols: Nature's Gift as dietary phytoconstituents against different human ailments
- date: 2019-12-29
- words: 5310
- flesch: 54
- summary: Yamada H, Watanabe H: Tea polyphenols in preventing cardiovascular diseases. Yamada H, Watanabe H: Tea polyphenols in preventing cardiovascular diseases.
- keywords: 2012; acids; action; activity; age; aging; anticancer; antidiabetic; antioxidant; bioavailability; biol; biotechnology; blood; bond; cancer; cancerous; cardiovascular; cardiovascular diseases; catechin; cell; chem; coffee; components; compounds; constituents; consumption; daily; death; derivatives; diabetes; dietary; different; diseases; effects; factors; figure; flavonoids; food; free; fruits; glucose; grapes; green; health; high; http://dx.doi.org/; human; important; intake; intestine; journal; lignans; low; mechanism; med; metab; natural; nepal; nepjol.info/index.php/njb; neurodegenerative; nutr; oxidative; phenolic; phenyl; plant; polyphenols; potential; prevention; process; products; promising; properties; quercetin; radicals; range; red; research; resveratrol; rich; risk; role; scavenging; society; sources; stilbene; stress; studies; study; synthesis; tea; thapa; vegetables; vol; wide; wine
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- njb-69
- author: Maharjan, Elina; Shakya, Pooja; Bhattachan, Balkrishna; Baral, Bharat Prasad; Shrestha, Dhiraj
- title: Phenotypic characterization of beta-lactamases producing Gram-negative bacteria in a tertiary hospital, Nepal
- date: 2019-12-29
- words: 4319
- flesch: 61
- summary: [17] and 4% Nepal et al in 2017 Poudyal S, Bhatta DR, Shakya G, Upadhyaya B, Dumre SP, Buda GC, et al: Extended- Spectrum beta-lactamase producing multidrug resistant clinical bacterial isolates at National Public Health Laboratory, Nepal.
- keywords: abr; acinetobacter; aeruginosa; antibiotic; antimicrobial; bacteria; beta; biotechnology; care; cefpodoxime; center; clinical; coli; control; data; disc; effective; esbl; extended; gram; health; higher; hospital; imipenem; isolates; journal; klebsiella; laboratory; lactamase; maharjan; management; mbl; mdr; metallo; method; microbiol; negative; nepal; nepjol.info/index.php/njb; njb; pneumoniae; positive; producers; production; resistance; samples; society; spectrum; spp; strains; study; table; test; total; vol; zoi
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- njb-70
- author: Kumari, Rinki; Ilango, K.; Singh, G.P.I.; Dubey, G.P.
- title: Pharmacological In vivo test to evaluate the antidepressant activity of polyherbal formulation
- date: 2019-12-29
- words: 6282
- flesch: 52
- summary: The results indicated that polyherbal formulation exhibited significant antidepressant activity, as indicated by its ability to decrease force swim stress, induced immobility time in rats as well as restoring the biogenic amines to normal level that were altered by the swim induced stress in whole rat brain. The present study can be summarized by following finding: 1. the force swim stress procedure caused depressive-like behavior in treatment rats, as observed by different assays such as mirror chamber test and elevated plus maze test; 2. significant reduction in depressive- like behaviors was evident in the stressed rats treated with PF and Sertraline (positive control); 3. forced swim test procedures imbalance the neurotransmitter and induced inflammatory injury along with oxidative damage by increasing lipid peroxidation in the treated rats and 4. PF and Sertraline treatments exerted protective effects against forced swim stress and increase the level of neurotransmitter and decrease the glutathione in the rat brain.
- keywords: 14th; 28th; 7th; acetylcholine; acid; activities; activity; animals; antidepressant; antioxidant; anxiety; arbortristis; behavioral; biochemical; biol; biotechnology; body; brain; chronic; cognitive; content; control; damage; data; days; decrease; depression; different; disorders; drug; effect; entries; extract; figure; forced; formulation; fst; glutathione; group; hippophae; immobility; increase; journal; kumari; level; life; like; lipid; major; mao; melatonin; mice; min; model; monoamine; nepal; nepjol.info/index.php/njb; neurotransmitter; njb; noradrenalin; number; nyctanthes; ocimum; open; oxidase; oxidative; peroxidation; pharmacol; plants; polyherbal; positive; potential; present; rats; reduced; reduction; results; reuptake; role; salcifolia; selective; serotonin; sertraline; significant; society; somnifera; standard; stressed; studies; study; swim; system; test; time; treatment; university; values; vol; water; withania
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- njb-71
- author: Chhetri, Bijay
- title: Nematode Fauna Associated with Kiwi (Actinidia delicosa, Chev.) Plants in Machchhegaun, Kathmandu, Nepal
- date: 2019-12-29
- words: 3321
- flesch: 60
- summary: No any published data about study of plant nematodes was found from study area. So, these four order of plant nematodes have been reported for the first time associated with Kiwi plants from Machchhegaun in Nepal.
- keywords: anterior; area; biotechnology; central; chhetri; crop; discolaimus; distribution; dorylaimus spp; eucephalobus spp; figure; free; helicotylenchus; helicotylenchus spp; iotonchus spp; journal; kathmandu; kiwi; kiwi plants; living; machchhegaun; magnification; mesorhabditis spp; mononchus; nematodes; nepal; nepjol.info/index.php/njb; njb; number; order; organic; parahadronchus; parahadronchus spp; parasitic; plants; posterior; predatory; present; production; region; rhizosphere; samples; society; soil; species; spp; study; total; tylenchida; vol
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- njb-74
- author: Pandey, Binayak Raj; Shrestha, Angela; Sharma, Nisha; Shrestha, Bhupal Govinda
- title: Evaluation of Phytochemical, Antimicrobial, Antioxidant Activity and Cytotoxic Potentials of Agave americana
- date: 2019-12-29
- words: 4482
- flesch: 54
- summary: Figure 1 Graphical comparison of antimicrobial activity of A. americana extract against different clinical isolates. The graph shows antioxidant assay of A. americana extract in comparison to ascorbic acid as the standard.
- keywords: activities; activity; agave; agave americana; americana; antibiotics; antimicrobial; antioxidant; assay; aureus; bacillus; biotechnology; breast; calculated; cancer; cell; clinical; concentration; control; cytotoxic; cytotoxicity; death; different; dmso; dpph; ethnomedicinal; extract; extraction; figure; flavonoids; ic50; journal; line; medicinal; medicine; method; negative; nepal; nepjol.info/index.php/njb; njb; non; pandey; phytochemical; plant; presence; properties; radical; research; resistant; results; saponins; scavenging; screening; shrestha; society; standard; study; table; thuringiensis; value; vol
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- njb-75
- author: Shakya, Shubha Ratna; Labh, Shyam Narayan
- title: Ethanol Extract of Choerospondias axillaris Fruit Pulp Enhances Haematological Parameters in Oncorhynchus mykiss Cultured in Nepal
- date: 2019-12-29
- words: 5833
- flesch: 60
- summary: In intensive aquaculture stress level in fish impairing immune responses against pathogens leads to disease outbreaks in fishes Discussion In fishes, blood is a patho-physiological reflector of the entire body and the haematological parameters such as leucocyte count, erythrocyte count, hematocrit and hemoglobin are routinely tested to monitor the health status of fish and any deviation from the normal ranges may indicate a disturbance in the physiological process.
- keywords: 2003; agents; analysis; antibiotics; aquaculture; axillaris; biol; biotechnology; blood; cage; cells; changes; chemical; choerospondias; cold; compounds; concentration; content; control; count; diet; dietary; different; disease; drugs; effective; effects; environment; experimental; extract; factors; fed; feed; fish; fishes; fruit; group; growth; haematocrit; haematological; haematological parameters; haematology; haemoglobin; hct; health; herbal; herbs; higher; immune; immunity; increase; india; ingredients; journal; kg-1; labh; lapsi; maximum; mchc; mcv; mean; medicinal; minimum; mykiss; natural; nepal; nepjol.info/index.php/njb; nile; njb; non; oil; oncorhynchus; parameters; phyllanthus; physiological; plants; present; production; pulp; rainbow; rbc; res; resistance; response; sci; shakya; shellfish; significant; similar; society; specific; status; stimulants; stress; study; system; table; tilapia; total; trout; use; value; vitamin; vol; water; white
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- njb-76
- author: Shrestha, Dhiraj; Thapa, Pratigya; Bhandari, Dinesh; Bhattachan, Balkrishna; Parajuli, Hiramani; Chaudary, Prakash; Sharma, Vijay Kumar; Shah, Pradeep Kumar
- title: Detection of Pyuria by Microscopic Urinalysis as a Marker of Pediatric Urinary Tract Infection
- date: 2019-12-29
- words: 3234
- flesch: 56
- summary: We confirmed 64 (18.1%) UTI cases by culture, the gold standard for UTI diagnosis. Fever was the most common clinical manifestation in UTI cases.
- keywords: biotechnology; cases; children; clinical; common; culture; data; diagnosis; febrile; female; fever; health; healthcare; infants; infection; journal; kathmandu; laboratory; male; marker; microbiology; microscopic; microscopic urinalysis; negative; nepal; nepjol.info/index.php/njb; njb; patients; pediatric; positive; predictive; pyuria; samples; sensitivity; society; specificity; study; symptoms; table; test; total; tract; treatment; urinalysis; urinary; urinary tract; urine; uti; value; vol
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- njb-77
- author: Agrahari, Gaurav; Koirala, Amrit; Thapa, Roshan; Chaudhary, Mahesh Kumar; Tuladhar, Reshma
- title: Antimicrobial Resistance Patterns and Plasmid Profiles of Methicillin Resistant Staphylococcus aureus Isolated from Clinical Samples
- date: 2019-12-29
- words: 4008
- flesch: 49
- summary: Terry Alli OA, Akinloye O, Rowley DA, Butcher PD: A comparative assessment of ribosomal DNA polymorphisms in methicillin resistant Staphylococcus aureus (MRSA) epidemiology. ORIGINAL RESEARCH ARTICLE Antimicrobial Resistance Patterns and Plasmid Profiles of Methicillin Resistant Staphylococcus aureus Isolated from Clinical Samples Gaurav Agrahari1, Amrit Koirala1, Roshan Thapa1, Mahesh Kumar Chaudhary2 and Reshma Tuladhar1* 1Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal, 2KIST Medical College, Imadol, Kathmandu, Nepal, Abstract Methicillin-resistant Staphylococcus aureus (MRSA), showing resistance to several antibiotics is a global health problem associated with considerable mortality and morbidity.
- keywords: agarose; agrahari; alkaline; analysis; antibiotic; antimicrobial; aureus; biotechnology; care; cefoxitin; clinical; coagulase; college; community; department; detection; different; diffusion; disc; dna; epidemiology; figure; gel; hospital; imadol; isolated; journal; kathmandu; lysis; meca; medical; methicillin; method; microbiology; molecular; mrsa; nepal; nepjol.info/index.php/njb; njb; patients; pattern; penicillin; plasmid; positive; prevalence; profile; resistant; resistant staphylococcus; s. aureus; samples; sensitivity; society; specimens; standard; staphylococcus; staphylococcus aureus; strains; studies; study; susceptibility; techniques; test; vancomycin; vol
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- njb-82
- author: Manzum, Amika Ahmed; Mamun, Md Arafat Al
- title: Isolation of Bacillus spp. Bacteria from Soil for Production of Cellulase
- date: 2019-01-15
- words: 2790
- flesch: 54
- summary: [8].Therefore in this study an investigation was undertaken to isolate Bacillus bacteria capable of producing cellulase enzyme. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples.
- keywords: activity; api; bacillus; bacteria; biotechnology; cellulase; cellulolytic; culture; doi; enzyme; food; glucose; industrial; isolates; isolation; journal; kit; large; mamun; manzum; media; medium; microbiol; min; nepal; nepjol.info/index.php/njb; njb; positive; production; research; results; samples; society; soil; species; spp; strains; study; test; vol
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- njb-83
- author: Baniya, Amina; Singh, Sushma; Singh, Minu; Nepal, Pragya; Adhikari, Mahesh; Aryal, Sagar; Adhikari, Anurag
- title: Isolation and Screening of Antibiotics Producing Streptomyces spp from the Soil Collected around the Root of Alnus nepalensis from Godawari
- date: 2019-01-15
- words: 4649
- flesch: 51
- summary: NASA 108(ZOI-29.66mm) and NASA 113(ZOI-26.4mm) showed antibacterial activity against Imepenem resistant E. faecalis (Figure 2). NASA 103, NASA 104, NASA 105, NASA 106, NASA 107, NASA 109, NASA 110, NASA 111, NASA 114, NASA 115, NASA 201, NASA 301, NASA 302, NASA 305, Nepal Journal of Biotechnology.
- keywords: + +; actinomycetes; activity; agar; alder; alnus; antibacterial; antibiotics; antimicrobial; baniya; biotechnology; characterization; coli; culture; dark; different; diversity; extraction; fermentation; figure; genome; gray; growth; imepenem; inhibition; isolates; isolation; journal; klebsiella; lalitpur; mdr; mdrs; media; methicillin; method; microbial; microorganisms; mrsa; multidrug; mycelium; nasa; nepal; nepalensis; nepjol.info/index.php/njb; njb; non; number; pathogens; pneumoniae; primary; research; resistant; rhizosphere; screening; secondary; slimey; society; soil; species; starch; strains; streptomyces; substrate; table; treatment; tree; vancomycin; vol; vre; white; whitish; yellow +; zoi; zone
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- njb-84
- author: Kumar, Ashok; Sharma, Palvi; Mahajan, Anupama; Begum, Zarina
- title: In Vitro Comparative Study of Antioxidant and Antibacterial Activity of Selected Dietary Plants
- date: 2019-01-15
- words: 3729
- flesch: 48
- summary: Due to drug resistance of pathogens, efforts have been made to find their substitute for treatment of diseases and with knowledge of antimicrobial activity of plant extracts, they may play a significant role in cure of microbial diseases [16]. 10mg/ml of Ascorbic acid used as reference for compare results of plant extracts.
- keywords: ability; activities; activity; aeuroginosa; agar; alkaloids; aloe; analysis; antibacterial; antimicrobial; antimicrobial activity; antioxidant; antioxidant activity; ascorbic; auerus; bacillus; biotechnology; bud; coli; diffusion; effect; escherichia; extract; flavonoids; flower; food; free; garlic; ginger; glycosides; growth; herbal; inhibition; journal; kumar; maximum; medicinal; method; mtcc; nepal; nepjol.info/index.php/njb; njb; phytochemical; plants; pseudomonas; radical; research; result; scavenging; sci; science; screening; singh; society; species; spectrophotometric; standard; staphylococcus; study; subtilis; table; terpenoids; test; turmeric; vol
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- njb-85
- author: Shah, Gyanendra Bikram; Gurung, Krishna; Baral, Bharat Prasad; Gurung, Ram Raja; Bhattachan, Balkrishna
- title: Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors
- date: 2019-01-15
- words: 3578
- flesch: 63
- summary: ©NJB, Biotechnology Society of Nepal 33 Nepjol.info/index.php/njb Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors Gyanendra Bikram Shah1, 2, Krishna Gurung1, Bharat Prasad Baral1, Ram Raja Gurung3, Blood donors in Pokhara Valley were screened for anti-HCV antibodies, anti-HBV antibodies using third generation ELISA kits and automated ELISA Processor in serology laboratory at Central Blood Transfusion Service (CBTS) of Nepal Red Cross Society (NRCS) in Pokhara, Nepal.
- keywords: age; biotechnology; blood; blood donors; cancer; chronic; cirrhosis; criteria; cross; data; diseases; distribution; donation; donors; elisa; factors; female; gender; group; hbv; hcv; health; hepatitis; higher; highest; infection; infection rate; journal; liver; method; nepal; nepjol.info/index.php/njb; njb; old; patients; people; pokhara; rapid; rate; risk; seroprevalence; shah; significant; society; study; table; tattoo; test; total; transfusion; virus; vol; volunteer; wise; year
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- njb-86
- author: Baral, Bharat Prasad; Koirala Sharma, Binita; Gurung, Krishna; Gurung, Ram Raja; Supram, Hosuru Subramanava; Bhattachan, Balkrishna
- title: Fecal carriage of Extended Spectrum β-Lactamases (ESBL) Producing Escherichia coli and Klebsiella spp. among School Children in Pokhara, Nepal
- date: 2019-01-15
- words: 3561
- flesch: 56
- summary: Conversely, the findings of another study [20] showed the variable result with E. coli isolates, 33(9%) were ESBL producers. This study aimed to determine the prevalence of ESBL producing E. coli and Klebsiella spp.
- keywords: antibiotics; antimicrobial; bacteria; baral; biotechnology; care; cefotaxime; ceftazidime; children; clinical; coli; collected; control; data; e. coli; esbl; escherichia; extended; figure; gram; hospital; infections; isolates; journal; klebsiella; klebsiella spp; laboratory; lactamase; mdr; nepal; nepjol.info/index.php/njb; njb; organisms; patients; pneumoniae; pokhara; positive; prevalence; producer; resistance; samples; school; society; spectrum; spp; stool; study; susceptibility; test; total; vol
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- njb-87
- author: Aryal, Sajesan; Hada, Aroj; Hamal, Sanjay; Prajapati, Abhishek; Timilsina, Paras Mani; Adhikari, Sandeep
- title: Development of Cost Optimized Horizontal Gel Electrophoresis Running Unit for Developing Countries (Nepal)
- date: 2019-01-15
- words: 2958
- flesch: 61
- summary: *Corresponding Author Email: acsandeep2014@gmail.com Introduction In graduate courses and research environment, the use of gel electrophoresis seems to be an expensive technique. This protocol upon optimization can be effectively embraced by the high schools, colleges and even universities to demonstrate the basic principle of gel electrophoresis and provide each student with an equal opportunity to get acquainted with the physical as well as working mechanisms of the gel electrophoresis equipment at a low cost.
- keywords: agarose; aluminum; anode; bands; biotechnology; box; buffer; cathode; cost; countries; dna; electrodes; electrophoresis; expensive; figure; fragments; gel; hindiii; image; laboratory; ladder; lambda; migration; neb; nepal; nichrome; plastic; platinum; run; safe; samples; separation; standard; students; tray; unit; usd; use; wires
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- njb-91
- author: Shakya, Shubha Ratna
- title: Effect of Herbs and Herbal Products Feed Supplements on Growth in Fishes: A Review
- date: 2017-12-31
- words: 4198
- flesch: 60
- summary: An artificial feed used in the aquaculture improves fish growth with maximum weight in short time [3]. New substances are added in fish feed to improve feed conversion efficiency that result in fish growth [4].
- keywords: activity; additives; allium; antibiotics; aquaculture; better; biotechnology; body; carp; carpio; catla; common; composition; consumption; control; conversion; cyprinus; diet; dietary; different; digestive; diseases; effect; efficiency; egypt; fed; feed; fenugreek; fingerlings; fish; fish growth; fishes; garlic; growth; growth performance; health; herbal; herbs; immunity; improved; increase; indian; journal; labeo; major; maximum; medicinal; mossambicus; nepal; nepjol.info/index.php/njb; nile; niloticus; njb; oreochromis; oreochromis niloticus; performance; plants; powder; production; products; protein; quality; rate; red; response; rohita; sativum; sci; sgr; shakya; society; stress; supplementation; supplemented; survival; tilapia; university; use; utilization; vitamin; vol; weight; world
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- njb-92
- author: Giri, Gorakh Raj; Timilsina, Uddhav
- title: Apoptosis: Implications in Viral and Mycobacterium tuberculosis infections
- date: 2017-12-31
- words: 7305
- flesch: 50
- summary: Both viral and Mycobacterium tuberculosis (Mtb) infections modulate host cell apoptosis for their benefits [6,9–11]. Thus, evidence suggests that host cell apoptosis is crucial for host resistance to Mtb infection.
- keywords: 1997; acad; activation; active; adenovirus; alveolar; anti; antiapoptotic; apoptosis; apoptotic; bad; bak; baltim; barr; bax; bcl2; bh3; bid; binding; biol; biotechnology; blocks; caspase; cell; cell apoptosis; cell death; chem; chen; complex; cytochrome; cytomegalovirus; death; degradation; dependent; dna; dnase; domains; epstein; et al; executioner; expression; extrinsic; factor; family; fas; fasl; figure; flip; formation; function; genes; giri; granzyme; hiv-1; host; human; immune; immunol; induction; infected; infection; inhibition; inhibits; initiator; interaction; intracellular; intrinsic; journal; large; leading; lee; ligand; like; macrophages; mechanisms; members; membrane; microbiol; mitochondrial; modulation; mol; molecular; mtb; mycobacterium; mycobacterium tuberculosis; natl; necrosis; nepal; nepjol.info/index.php/njb; new; njb; p53; pathological; pathway; permeability; physiol; proapoptotic; proc; protein; receptor; regulation; release; response; role; sci; signals; society; stimuli; survival; tat; timilsina; tnf; tnfα; trail; transmembrane; trigger; tuberculosis; tumor; upregulates; viral; virulent; virus; viruses; vol; wang; xiap
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- njb-93
- author: Rimal, Ujjwal; Thapa, Shovana; Maharjan, Roshani
- title: Prevalence of Extended Spectrum Beta-Lactamase Producing Escherichia coli and Klebsiella species from Urinary Specimens of Children attending Friendship International Children’s Hospital
- date: 2017-12-31
- words: 4741
- flesch: 55
- summary: In our study, 25.5% of E. coli isolates and 12.5% of Klebsiella pneumoniae isolates were ESBL producer. Regular and routine monitoring of ESBL producing isolates is essential.
- keywords: amoxicillin; antibiotic; antimicrobial; bacterial; beta; biotechnology; ceftazidime; ceftriaxone; children; ciprofloxacin; clinical; coli; cotrimoxazole; drug; e. coli; enzymes; esbl; escherichia; escherichia coli; extended; female; growth; hospital; imipenem; infections; international; isolates; journal; kathmandu; klebsiella; klebsiella pneumoniae; laboratory; lactamase; nepal; nepjol.info/index.php/njb; nitrofurantoin; njb; oxytoca; patients; pattern; pneumoniae; positive; prevalence; producers; rate; resistance; result; rimal; samples; sensitive; significant; similar; society; spectrum; spp; strains; studies; study; susceptibility; table; test; total; tract; urinary; urine; vol
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- njb-94
- author: Chaudhary, Pradip Kumar; Shrestha, Mitesh; Poudel, Bal Hari; Adhikari, Mahesh Kumar
- title: In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
- date: 2017-12-31
- words: 2639
- flesch: 59
- summary: ©NJB, Biotechnology Society of Nepal 28 Nepjol.info/index.php/njb induce development of fruiting bodies from wild edible mushroom V. bombycina. A variety of waste materials have been used for cultivation of Volvariella mushroom that include paddy straw, water hyacinth, oil palm bunch and oil palm pericarp waste, banana waste, sawdust, cotton waste, sugar cane bagasse, composted mixtures of tropical wood wastes and pineapple skin waste.
- keywords: 25oc; bags; biotechnology; bodies; bombycina; chaudhary; collected; cultivation; culture; days; development; edible; environmental; factors; figure; formation; fruiting; growth; incubation; journal; medicinal; mushroom; mycelium; nepal; nepjol.info/index.php/njb; njb; paddy; pink; plant; populus; primordial; pure; silky; society; spawn; species; stage; straw; substrates; temperature; tree; university; volvacea; volvariella; volvariella bombycina; waste; weeks; white; wild
- versions: original; plain text
- njb-95
- author: Subedi, Munal; Magar, Mamata Gharti; Rajbhandari, Gita Shrestha
- title: Assessment of Quality of Underground Drinking Water: Very near (≤20 meters) and Far (>50 meters) from the River
- date: 2017-12-31
- words: 3887
- flesch: 59
- summary: 300 ml of underground water samples were collected from 100 different households located near site (≤20 meters) and far site (>50 meters) from Bagmati river. However, in areas ≤10 meter distance, pH ranged from 7.3-8.9, whereas in water samples from areas >50 meters distance pH ranged from 6.9-7.9.
- keywords: ammonia; analysis; areas; bagmati; biological; biotechnology; chloride; coliform; conductivity; contamination; content; different; distance; drinking; far; fecal; figure; health; high; higher; households; journal; kathmandu; meters; meters distance; method; near; nearer; nepal; nepjol.info/index.php/njb; nitrate; njb; parameters; physiochemical; polluted; pollution; purpose; quality; range; river; samples; sites; society; sources; standard; study; subedi; treatment; underground; underground water; valley; values; vol; water; ≤10; ≤20
- versions: original; plain text
- njb-96
- author: Thapa, Kabi; Bhattachan, Balkrishna; Gurung, Raja Ram; Katuwal, Archana; Khadka, Jagat Bahadur
- title: Antibiotic Resistance Pattern of Shigella spp. Among Gastroenteritis Patients at Tertiary Care Hospital in Pokhara, Nepal
- date: 2017-12-31
- words: 4007
- flesch: 57
- summary: ©NJB, Biotechnology Society of Nepal 14 Nepjol.info/index.php/njb Antibiotic Resistance Pattern of Shigella spp. The main aim of our study is to isolate and identify Shigella spp.
- keywords: acid; age; ampicillin; antibiotic; antimicrobial; bacterial; biotechnology; boydii; cases; children; ciprofloxacin; clinical; collected; countries; dis; disease; drugs; dysenteriae; flexneri; gastroenteritis; gram; group; hospital; india; infection; isolated; journal; khan; laboratory; med; method; nalidixic; negative; nepal; nepjol.info/index.php/njb; njb; old; patients; pattern; positive; prevalence; rate; regional; resistance; s. sonnei; sample; shigella; shigella spp; shigellosis; society; sonnei; species; spp; stool; study; test; thapa; total; trimoxazole; vol; western; years
- versions: original; plain text
- njb-97
- author: Thusa, Rojeena; Mulmi, Sushika
- title: Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata
- date: 2017-12-31
- words: 4907
- flesch: 59
- summary: Test for Reducing Sugar To 0.5ml of plant extract, 1ml of water and 5-8 drops of Fehling’s solution was added and heated over water bath. Test for Cardiac Glycosides 5ml of plant extract was treated with 2ml of glacial acetic acid containing one drop of FeCl3 solution.
- keywords: + +; acetate; acid; activity; antimicrobial; aristata; aureus; authentic; berberine; berberis; biotechnology; brine; cm-1; color; compound; dark; department; different; ethyl; extract; figure; green; hexane; isolated; isolation; journal; leaf; mahonia; mahonia nepalensis; methanol; mn2; mn3; mulmi; nepalensis; nepjol.info/index.php/njb; njb; orange; phytochemical; plant; precipitate; presence; r.f; red; resistant; sample; screening; shrimp; society; solution; spectrum; spot; staphylococcus; stem; sticky; stretch; table; test; thusa; tlc; value; vol; yellow; yield
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- njb-98
- author: Devkota, Hari Prasad; Miyazaki, Sena; Yahara, Shoji
- title: Amentoflavone and Kaempferol Glycosides from the Aerial Parts of Cissampelos pareira
- date: 2017-12-31
- words: 1870
- flesch: 66
- summary: Keywords: Cissampelos pareira; Menispermaceae; Batulpate; Flavonoids; Amentoflavone *Corresponding Author Email: devkotah@kumamoto-u.ac.jp Introduction Cissampelos pareira L. (Family: Menispermaceae), commonly known as “Batulpate” in Nepal, is a climbing herb with perennial root-stock. Borthakur M, Devi N, Mahanta R: Antifertility activity of the methanolic leaf extract of Cissampelos pareira in female albino mice.
- keywords: aerial; alkaloids; amentoflavone; biotechnology; chemical; cissampelos; compounds; constituents; extract; family; flavonoids; gel; glucopyranoside; glucuronopyranoside; glycosides; isolated; kaempferol; menispermaceae; meoh; nepal; nmr; o--d; pareira; parts; plant; silica; table; water
- versions: original; plain text
- njb-99
- author: Bastakoti, Srijana; Belbase, Shiva; Manandhar, Shrinkhala; Arjyal, Charu
- title: Trichoderma species as Biocontrol Agent against Soil Borne Fungal Pathogens
- date: 2017-12-31
- words: 4403
- flesch: 49
- summary: Keywords: Biocontrol, Trichoderma species, inhibition percentage, soil borne pathogens and dual culture technique *Correspondence Author Email: srijanabastakoti33@gmail.com Introduction Soil borne plant pathogenic fungi cause heavy crop losses all over the world. Biological control of plant diseases, especially those caused by soil borne plant pathogens and nematodes, by microorganisms has been considered a more natural and environmentally acceptable alternative to the existing chemical treatment methods [3].
- keywords: action; agent; agricultural; antibiotics; bastakoti; biocontrol; biological; biotechnology; borne; chemical; collected; colony; culture; days; diameter; different; disease; dual; effective; efficacy; environment; enzymes; figure; fungal; fungal pathogens; fungi; fusarium; growth; incubation; inhibition; isolates; isolation; journal; nepal; nepjol.info/index.php/njb; njb; pathogens; pathology; pda; percentage; plant; plate; potential; presence; radial; region; research; rhizoctonia; rolfsii; samples; sclerotionia; sclerotium; sclerotium rolfsii; society; soil; solani; species; study; technique; test; trichoderma; trichoderma species; vol
- versions: original; plain text