id	sid	tid	token	lemma	pos
njb-38	1	1	nepal	nepal	ADJ
njb-38	1	2	journal	journal	NOUN
njb-38	1	3	of	of	ADP
njb-38	1	4	biotechnology	biotechnology	NOUN
njb-38	1	5	.	.	PUNCT
njb-38	2	1	d	d	X
njb-38	2	2	e	e	PROPN
njb-38	2	3	c	c	PROPN
njb-38	2	4	.	.	PUNCT
njb-38	3	1	2	2	NUM
njb-38	3	2	0	0	NUM
njb-38	3	3	1	1	NUM
njb-38	3	4	5	5	NUM
njb-38	3	5	vol	vol	NOUN
njb-38	3	6	.	.	PUNCT
njb-38	4	1	3	3	NUM
njb-38	4	2	,	,	PUNCT
njb-38	4	3	no	no	INTJ
njb-38	4	4	.	.	PUNCT
njb-38	5	1	1:22	1:22	NUM
njb-38	5	2	-	-	SYM
njb-38	5	3	28	28	NUM
njb-38	5	4	issn	issn	PROPN
njb-38	5	5	2091	2091	NUM
njb-38	5	6	-	-	SYM
njb-38	5	7	1130	1130	NUM
njb-38	5	8	(	(	PUNCT
njb-38	5	9	print	print	NOUN
njb-38	5	10	)	)	PUNCT
njb-38	5	11	/	/	PUNCT
njb-38	5	12	issn	issn	PROPN
njb-38	5	13	2467	2467	NUM
njb-38	5	14	-	-	SYM
njb-38	5	15	9319	9319	NUM
njb-38	5	16	(	(	PUNCT
njb-38	5	17	online	online	ADJ
njb-38	5	18	)	)	PUNCT
njb-38	5	19	original	original	ADJ
njb-38	5	20	research	research	NOUN
njb-38	5	21	article	article	NOUN
njb-38	5	22	©	©	PROPN
njb-38	5	23	njb	njb	PROPN
njb-38	5	24	,	,	PUNCT
njb-38	5	25	biotechnology	biotechnology	NOUN
njb-38	5	26	society	society	NOUN
njb-38	5	27	of	of	ADP
njb-38	5	28	nepal	nepal	ADJ
njb-38	5	29	22	22	NUM
njb-38	5	30	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-38	5	31	heat	heat	NOUN
njb-38	5	32	shock	shock	NOUN
njb-38	5	33	protein	protein	NOUN
njb-38	5	34	70	70	NUM
njb-38	5	35	(	(	PUNCT
njb-38	5	36	hsp70	hsp70	NOUN
njb-38	5	37	)	)	PUNCT
njb-38	5	38	expression	expression	NOUN
njb-38	5	39	in	in	ADP
njb-38	5	40	antimony	antimony	NOUN
njb-38	5	41	susceptible	susceptible	ADJ
njb-38	5	42	/	/	SYM
njb-38	5	43	resistant	resistant	ADJ
njb-38	5	44	clinical	clinical	ADJ
njb-38	5	45	isolates	isolate	NOUN
njb-38	5	46	of	of	ADP
njb-38	5	47	leishmania	leishmania	PROPN
njb-38	5	48	donovani	donovani	PROPN
njb-38	5	49	mahendra	mahendra	PROPN
njb-38	5	50	maharjan1,2	maharjan1,2	PROPN
njb-38	5	51	*	*	PROPN
njb-38	5	52	and	and	CCONJ
njb-38	5	53	rentala	rentala	PROPN
njb-38	5	54	madhubala2	madhubala2	PROPN
njb-38	6	1	1central	1central	NUM
njb-38	6	2	department	department	NOUN
njb-38	6	3	of	of	ADP
njb-38	6	4	zoology	zoology	PROPN
njb-38	6	5	,	,	PUNCT
njb-38	6	6	tribhuvan	tribhuvan	PROPN
njb-38	6	7	university	university	PROPN
njb-38	6	8	,	,	PUNCT
njb-38	6	9	kirtipur	kirtipur	PROPN
njb-38	6	10	,	,	PUNCT
njb-38	6	11	kathmandu	kathmandu	NOUN
njb-38	6	12	,	,	PUNCT
njb-38	6	13	nepal	nepal	ADJ
njb-38	6	14	2	2	NUM
njb-38	6	15	school	school	NOUN
njb-38	6	16	of	of	ADP
njb-38	6	17	life	life	NOUN
njb-38	6	18	sciences	science	NOUN
njb-38	6	19	,	,	PUNCT
njb-38	6	20	jawahar	jawahar	PROPN
njb-38	6	21	lal	lal	PROPN
njb-38	6	22	nehru	nehru	PROPN
njb-38	6	23	university	university	PROPN
njb-38	6	24	,	,	PUNCT
njb-38	6	25	new	new	PROPN
njb-38	6	26	delhi	delhi	PROPN
njb-38	6	27	,	,	PUNCT
njb-38	6	28	india	india	PROPN
njb-38	6	29	abstract	abstract	PROPN
njb-38	6	30	pentavalent	pentavalent	PROPN
njb-38	6	31	antimonials	antimonial	NOUN
njb-38	6	32	have	have	AUX
njb-38	6	33	long	long	ADV
njb-38	6	34	been	be	AUX
njb-38	6	35	the	the	DET
njb-38	6	36	first	first	ADJ
njb-38	6	37	line	line	NOUN
njb-38	6	38	of	of	ADP
njb-38	6	39	defence	defence	NOUN
njb-38	6	40	against	against	ADP
njb-38	6	41	leishmaniasis	leishmaniasis	NOUN
njb-38	6	42	,	,	PUNCT
njb-38	6	43	but	but	CCONJ
njb-38	6	44	resistance	resistance	NOUN
njb-38	6	45	has	have	AUX
njb-38	6	46	been	be	AUX
njb-38	6	47	reported	report	VERB
njb-38	6	48	in	in	ADP
njb-38	6	49	different	different	ADJ
njb-38	6	50	parts	part	NOUN
njb-38	6	51	of	of	ADP
njb-38	6	52	the	the	DET
njb-38	6	53	world	world	NOUN
njb-38	6	54	.	.	PUNCT
njb-38	7	1	pentavalent	pentavalent	NOUN
njb-38	7	2	antimony	antimony	NOUN
njb-38	7	3	is	be	AUX
njb-38	7	4	reduced	reduce	VERB
njb-38	7	5	into	into	ADP
njb-38	7	6	trivalent	trivalent	ADJ
njb-38	7	7	form	form	NOUN
njb-38	7	8	in	in	ADP
njb-38	7	9	the	the	DET
njb-38	7	10	cells	cell	NOUN
njb-38	7	11	and	and	CCONJ
njb-38	7	12	is	be	AUX
njb-38	7	13	a	a	DET
njb-38	7	14	potential	potential	ADJ
njb-38	7	15	inducer	inducer	NOUN
njb-38	7	16	of	of	ADP
njb-38	7	17	hsp70	hsp70	NOUN
njb-38	7	18	in	in	ADP
njb-38	7	19	l.	l.	PROPN
njb-38	7	20	donovani	donovani	PROPN
njb-38	7	21	.	.	PUNCT
njb-38	8	1	expression	expression	NOUN
njb-38	8	2	profile	profile	NOUN
njb-38	8	3	of	of	ADP
njb-38	8	4	hsp70	hsp70	NOUN
njb-38	8	5	in	in	ADP
njb-38	8	6	antimony	antimony	NOUN
njb-38	8	7	susceptible	susceptible	ADJ
njb-38	8	8	and	and	CCONJ
njb-38	8	9	resistant	resistant	ADJ
njb-38	8	10	l.	l.	PROPN
njb-38	8	11	donovani	donovani	PROPN
njb-38	8	12	isolates	isolate	NOUN
njb-38	8	13	were	be	AUX
njb-38	8	14	characterized	characterize	VERB
njb-38	8	15	by	by	ADP
njb-38	8	16	southern	southern	ADJ
njb-38	8	17	blot	blot	NOUN
njb-38	8	18	,	,	PUNCT
njb-38	8	19	northern	northern	ADJ
njb-38	8	20	blot	blot	NOUN
njb-38	8	21	and	and	CCONJ
njb-38	8	22	western	western	ADJ
njb-38	8	23	blot	blot	NOUN
njb-38	8	24	analysis	analysis	NOUN
njb-38	8	25	.	.	PUNCT
njb-38	9	1	hsp70	hsp70	NOUN
njb-38	9	2	gene	gene	NOUN
njb-38	9	3	copy	copy	NOUN
njb-38	9	4	number	number	NOUN
njb-38	9	5	,	,	PUNCT
njb-38	9	6	gene	gene	NOUN
njb-38	9	7	expression	expression	NOUN
njb-38	9	8	and	and	CCONJ
njb-38	9	9	hsp70	hsp70	NOUN
njb-38	9	10	protein	protein	NOUN
njb-38	9	11	expression	expression	NOUN
njb-38	9	12	was	be	AUX
njb-38	9	13	found	find	VERB
njb-38	9	14	uniform	uniform	NOUN
njb-38	9	15	in	in	ADP
njb-38	9	16	both	both	PRON
njb-38	9	17	antimony	antimony	NOUN
njb-38	9	18	sensitive	sensitive	ADJ
njb-38	9	19	and	and	CCONJ
njb-38	9	20	resistant	resistant	ADJ
njb-38	9	21	clinical	clinical	ADJ
njb-38	9	22	isolates	isolate	NOUN
njb-38	9	23	.	.	PUNCT
njb-38	10	1	in	in	ADP
njb-38	10	2	laboratory	laboratory	NOUN
njb-38	10	3	condition	condition	NOUN
njb-38	10	4	,	,	PUNCT
njb-38	10	5	leishmania	leishmania	PROPN
njb-38	10	6	cells	cell	NOUN
njb-38	10	7	respond	respond	VERB
njb-38	10	8	to	to	ADP
njb-38	10	9	antimonial	antimonial	ADJ
njb-38	10	10	drug	drug	NOUN
njb-38	10	11	stress	stress	NOUN
njb-38	10	12	by	by	ADP
njb-38	10	13	three	three	NUM
njb-38	10	14	fold	fold	NOUN
njb-38	10	15	over	over	ADP
njb-38	10	16	expression	expression	NOUN
njb-38	10	17	of	of	ADP
njb-38	10	18	the	the	DET
njb-38	10	19	hsp70	hsp70	PROPN
njb-38	10	20	protein	protein	NOUN
njb-38	10	21	.	.	PUNCT
njb-38	11	1	the	the	DET
njb-38	11	2	observed	observe	VERB
njb-38	11	3	results	result	NOUN
njb-38	11	4	indicated	indicate	VERB
njb-38	11	5	that	that	SCONJ
njb-38	11	6	hsp70	hsp70	NOUN
njb-38	11	7	play	play	VERB
njb-38	11	8	important	important	ADJ
njb-38	11	9	role	role	NOUN
njb-38	11	10	in	in	ADP
njb-38	11	11	stress	stress	NOUN
njb-38	11	12	tolerance	tolerance	NOUN
njb-38	11	13	against	against	ADP
njb-38	11	14	antimonial	antimonial	ADJ
njb-38	11	15	drug	drug	NOUN
njb-38	11	16	without	without	ADP
njb-38	11	17	differential	differential	ADJ
njb-38	11	18	expression	expression	NOUN
njb-38	11	19	in	in	ADP
njb-38	11	20	antimony	antimony	NOUN
njb-38	11	21	sensitive	sensitive	ADJ
njb-38	11	22	and	and	CCONJ
njb-38	11	23	resistance	resistance	NOUN
njb-38	11	24	clinical	clinical	ADJ
njb-38	11	25	isolates	isolate	NOUN
njb-38	11	26	of	of	ADP
njb-38	11	27	l.	l.	PROPN
njb-38	11	28	donovani	donovani	PROPN
njb-38	11	29	.	.	PUNCT
njb-38	12	1	keywords	keyword	NOUN
njb-38	12	2	:	:	PUNCT
njb-38	12	3	pentavalent	pentavalent	NOUN
njb-38	12	4	antimony	antimony	NOUN
njb-38	12	5	;	;	PUNCT
njb-38	12	6	hsp70	hsp70	NOUN
njb-38	12	7	;	;	PUNCT
njb-38	12	8	visceral	visceral	NOUN
njb-38	12	9	leishmaniasis	leishmaniasis	NOUN
njb-38	12	10	;	;	PUNCT
njb-38	12	11	drug	drug	NOUN
njb-38	12	12	resistance	resistance	NOUN
njb-38	12	13	;	;	PUNCT
njb-38	12	14	pfge	pfge	NOUN
njb-38	12	15	;	;	PUNCT
njb-38	12	16	hybridization	hybridization	NOUN
njb-38	12	17	*	*	PUNCT
njb-38	12	18	corresponding	correspond	VERB
njb-38	12	19	author	author	NOUN
njb-38	12	20	email	email	NOUN
njb-38	12	21	:	:	PUNCT
njb-38	12	22	mmaharjan@cdztu.edu.np	mmaharjan@cdztu.edu.np	PROPN
njb-38	12	23	introduction	introduction	NOUN
njb-38	12	24	visceral	visceral	NOUN
njb-38	12	25	leishmaniasis	leishmaniasis	NOUN
njb-38	12	26	is	be	AUX
njb-38	12	27	a	a	DET
njb-38	12	28	protozoan	protozoan	NOUN
njb-38	12	29	parasitic	parasitic	ADJ
njb-38	12	30	disease	disease	NOUN
njb-38	12	31	caused	cause	VERB
njb-38	12	32	by	by	ADP
njb-38	12	33	leishmania	leishmania	PROPN
njb-38	12	34	donovani	donovani	PROPN
njb-38	12	35	.	.	PUNCT
njb-38	13	1	leishmaniasis	leishmaniasis	NOUN
njb-38	13	2	constitutes	constitute	VERB
njb-38	13	3	a	a	DET
njb-38	13	4	major	major	ADJ
njb-38	13	5	public	public	ADJ
njb-38	13	6	health	health	NOUN
njb-38	13	7	problem	problem	NOUN
njb-38	13	8	with	with	ADP
njb-38	13	9	increasing	increase	VERB
njb-38	13	10	pattern	pattern	NOUN
njb-38	13	11	of	of	ADP
njb-38	13	12	disease	disease	NOUN
njb-38	13	13	burden	burden	NOUN
njb-38	13	14	[	[	X
njb-38	13	15	1,2	1,2	NUM
njb-38	13	16	]	]	PUNCT
njb-38	13	17	.	.	PUNCT
njb-38	14	1	it	it	PRON
njb-38	14	2	is	be	AUX
njb-38	14	3	a	a	DET
njb-38	14	4	neglected	neglect	VERB
njb-38	14	5	tropical	tropical	ADJ
njb-38	14	6	disease	disease	NOUN
njb-38	14	7	(	(	PUNCT
njb-38	14	8	ntd	ntd	ADV
njb-38	14	9	)	)	PUNCT
njb-38	14	10	which	which	PRON
njb-38	14	11	affects	affect	VERB
njb-38	14	12	mainly	mainly	ADV
njb-38	14	13	the	the	DET
njb-38	14	14	poor	poor	ADJ
njb-38	14	15	population	population	NOUN
njb-38	14	16	groups	group	NOUN
njb-38	14	17	,	,	PUNCT
njb-38	14	18	primarily	primarily	ADV
njb-38	14	19	in	in	ADP
njb-38	14	20	rural	rural	ADJ
njb-38	14	21	areas	area	NOUN
njb-38	14	22	.	.	PUNCT
njb-38	15	1	unfortunately	unfortunately	ADV
njb-38	15	2	,	,	PUNCT
njb-38	15	3	there	there	PRON
njb-38	15	4	is	be	VERB
njb-38	15	5	still	still	ADV
njb-38	15	6	lack	lack	NOUN
njb-38	15	7	of	of	ADP
njb-38	15	8	effective	effective	ADJ
njb-38	15	9	,	,	PUNCT
njb-38	15	10	affordable	affordable	ADJ
njb-38	15	11	and	and	CCONJ
njb-38	15	12	easy	easy	ADJ
njb-38	15	13	-	-	PUNCT
njb-38	15	14	to	to	ADP
njb-38	15	15	-	-	PUNCT
njb-38	15	16	use	use	VERB
njb-38	15	17	drugs	drug	NOUN
njb-38	15	18	for	for	ADP
njb-38	15	19	leishmaniasis	leishmaniasis	NOUN
njb-38	15	20	treatment	treatment	NOUN
njb-38	15	21	.	.	PUNCT
njb-38	16	1	since	since	SCONJ
njb-38	16	2	vaccine	vaccine	NOUN
njb-38	16	3	against	against	ADP
njb-38	16	4	leishmaniasis	leishmaniasis	NOUN
njb-38	16	5	is	be	AUX
njb-38	16	6	still	still	ADV
njb-38	16	7	under	under	ADP
njb-38	16	8	development	development	NOUN
njb-38	16	9	,	,	PUNCT
njb-38	16	10	the	the	DET
njb-38	16	11	control	control	NOUN
njb-38	16	12	lies	lie	VERB
njb-38	16	13	solely	solely	ADV
njb-38	16	14	on	on	ADP
njb-38	16	15	chemotherapy	chemotherapy	NOUN
njb-38	16	16	[	[	PUNCT
njb-38	16	17	3	3	NUM
njb-38	16	18	]	]	X
njb-38	16	19	.	.	PUNCT
njb-38	17	1	however	however	ADV
njb-38	17	2	,	,	PUNCT
njb-38	17	3	emergence	emergence	NOUN
njb-38	17	4	of	of	ADP
njb-38	17	5	drug	drug	NOUN
njb-38	17	6	resistance	resistance	NOUN
njb-38	17	7	in	in	ADP
njb-38	17	8	parasitic	parasitic	ADJ
njb-38	17	9	protozoa	protozoan	NOUN
njb-38	17	10	is	be	AUX
njb-38	17	11	becoming	become	VERB
njb-38	17	12	a	a	DET
njb-38	17	13	major	major	ADJ
njb-38	17	14	public	public	ADJ
njb-38	17	15	health	health	NOUN
njb-38	17	16	problem	problem	NOUN
njb-38	17	17	.	.	PUNCT
njb-38	18	1	heat	heat	NOUN
njb-38	18	2	shock	shock	NOUN
njb-38	18	3	proteins	protein	NOUN
njb-38	18	4	(	(	PUNCT
njb-38	18	5	hsps	hsps	NOUN
njb-38	18	6	)	)	PUNCT
njb-38	18	7	are	be	AUX
njb-38	18	8	highly	highly	ADV
njb-38	18	9	conserved	conserved	ADJ
njb-38	18	10	proteins	protein	NOUN
njb-38	18	11	found	find	VERB
njb-38	18	12	in	in	ADP
njb-38	18	13	both	both	PRON
njb-38	18	14	prokaryotic	prokaryotic	ADJ
njb-38	18	15	and	and	CCONJ
njb-38	18	16	eukaryotic	eukaryotic	ADJ
njb-38	18	17	cells	cell	NOUN
njb-38	18	18	.	.	PUNCT
njb-38	19	1	hsp70	hsp70	PROPN
njb-38	19	2	is	be	AUX
njb-38	19	3	a	a	DET
njb-38	19	4	molecular	molecular	ADJ
njb-38	19	5	chaperone	chaperone	NOUN
njb-38	19	6	which	which	PRON
njb-38	19	7	plays	play	VERB
njb-38	19	8	an	an	DET
njb-38	19	9	important	important	ADJ
njb-38	19	10	role	role	NOUN
njb-38	19	11	in	in	ADP
njb-38	19	12	protein	protein	NOUN
njb-38	19	13	folding	folding	NOUN
njb-38	19	14	and	and	CCONJ
njb-38	19	15	assembly	assembly	NOUN
njb-38	19	16	of	of	ADP
njb-38	19	17	polypeptides	polypeptide	NOUN
njb-38	19	18	within	within	ADP
njb-38	19	19	the	the	DET
njb-38	19	20	cell	cell	NOUN
njb-38	19	21	.	.	PUNCT
njb-38	20	1	when	when	SCONJ
njb-38	20	2	cells	cell	NOUN
njb-38	20	3	are	be	AUX
njb-38	20	4	exposed	expose	VERB
njb-38	20	5	to	to	ADP
njb-38	20	6	the	the	DET
njb-38	20	7	stressed	stressed	ADJ
njb-38	20	8	condition	condition	NOUN
njb-38	20	9	,	,	PUNCT
njb-38	20	10	the	the	DET
njb-38	20	11	proportion	proportion	NOUN
njb-38	20	12	of	of	ADP
njb-38	20	13	misfolded	misfolde	VERB
njb-38	20	14	proteins	protein	NOUN
njb-38	20	15	(	(	PUNCT
njb-38	20	16	mfps	mfps	PROPN
njb-38	20	17	)	)	PUNCT
njb-38	20	18	suddenly	suddenly	ADV
njb-38	20	19	increases	increase	VERB
njb-38	20	20	and	and	CCONJ
njb-38	20	21	the	the	DET
njb-38	20	22	cell	cell	NOUN
njb-38	20	23	reacts	react	VERB
njb-38	20	24	by	by	ADP
njb-38	20	25	synthesizing	synthesize	VERB
njb-38	20	26	hsps	hsp	NOUN
njb-38	20	27	to	to	PART
njb-38	20	28	assist	assist	VERB
njb-38	20	29	those	those	DET
njb-38	20	30	proteins	protein	NOUN
njb-38	20	31	in	in	ADP
njb-38	20	32	refolding	refolding	NOUN
njb-38	20	33	.	.	PUNCT
njb-38	21	1	the	the	DET
njb-38	21	2	stress	stress	NOUN
njb-38	21	3	response	response	NOUN
njb-38	21	4	is	be	AUX
njb-38	21	5	controlled	control	VERB
njb-38	21	6	primarily	primarily	ADV
njb-38	21	7	at	at	ADP
njb-38	21	8	the	the	DET
njb-38	21	9	transcription	transcription	NOUN
njb-38	21	10	level	level	NOUN
njb-38	21	11	by	by	ADP
njb-38	21	12	a	a	DET
njb-38	21	13	heat	heat	NOUN
njb-38	21	14	shock	shock	NOUN
njb-38	21	15	factor	factor	NOUN
njb-38	21	16	(	(	PUNCT
njb-38	21	17	hsf	hsf	NOUN
njb-38	21	18	)	)	PUNCT
njb-38	22	1	[	[	PUNCT
njb-38	22	2	4	4	NUM
njb-38	22	3	]	]	PUNCT
njb-38	22	4	.	.	PUNCT
njb-38	23	1	it	it	PRON
njb-38	23	2	has	have	AUX
njb-38	23	3	been	be	AUX
njb-38	23	4	shown	show	VERB
njb-38	23	5	that	that	DET
njb-38	23	6	transcript	transcript	NOUN
njb-38	23	7	level	level	NOUN
njb-38	23	8	of	of	ADP
njb-38	23	9	hsp70	hsp70	NOUN
njb-38	23	10	increased	increase	VERB
njb-38	23	11	in	in	ADP
njb-38	23	12	l.	l.	ADJ
njb-38	23	13	major	major	PROPN
njb-38	23	14	and	and	CCONJ
njb-38	23	15	in	in	ADP
njb-38	23	16	l.	l.	PROPN
njb-38	23	17	infantum	infantum	PROPN
njb-38	23	18	,	,	PUNCT
njb-38	23	19	in	in	ADP
njb-38	23	20	response	response	NOUN
njb-38	23	21	to	to	ADP
njb-38	23	22	elevated	elevated	ADJ
njb-38	23	23	temperature	temperature	NOUN
njb-38	23	24	conditions	condition	NOUN
njb-38	23	25	[	[	X
njb-38	23	26	5	5	NUM
njb-38	23	27	,	,	PUNCT
njb-38	23	28	6	6	NUM
njb-38	23	29	,	,	PUNCT
njb-38	23	30	7	7	NUM
njb-38	23	31	]	]	PUNCT
njb-38	23	32	.	.	PUNCT
njb-38	24	1	besides	besides	SCONJ
njb-38	24	2	elevated	elevated	ADJ
njb-38	24	3	temperature	temperature	NOUN
njb-38	24	4	,	,	PUNCT
njb-38	24	5	metals	metal	NOUN
njb-38	24	6	are	be	AUX
njb-38	24	7	also	also	ADV
njb-38	24	8	known	know	VERB
njb-38	24	9	to	to	PART
njb-38	24	10	be	be	AUX
njb-38	24	11	important	important	ADJ
njb-38	24	12	stress	stress	NOUN
njb-38	24	13	inducers	inducer	VERB
njb-38	24	14	in	in	ADP
njb-38	24	15	the	the	DET
njb-38	24	16	cells	cell	NOUN
njb-38	24	17	.	.	PUNCT
njb-38	25	1	antimonial	antimonial	ADJ
njb-38	25	2	compounds	compound	NOUN
njb-38	25	3	;	;	PUNCT
njb-38	25	4	pentostam	pentostam	NOUN
njb-38	25	5	and	and	CCONJ
njb-38	25	6	glucantime	glucantime	NOUN
njb-38	25	7	are	be	AUX
njb-38	25	8	still	still	ADV
njb-38	25	9	the	the	DET
njb-38	25	10	drug	drug	NOUN
njb-38	25	11	of	of	ADP
njb-38	25	12	choice	choice	NOUN
njb-38	25	13	in	in	ADP
njb-38	25	14	the	the	DET
njb-38	25	15	treatment	treatment	NOUN
njb-38	25	16	against	against	ADP
njb-38	25	17	all	all	DET
njb-38	25	18	forms	form	NOUN
njb-38	25	19	of	of	ADP
njb-38	25	20	leishmania	leishmania	NOUN
njb-38	25	21	infections	infection	NOUN
njb-38	25	22	[	[	PUNCT
njb-38	25	23	8	8	NUM
njb-38	25	24	,	,	PUNCT
njb-38	25	25	9	9	NUM
njb-38	25	26	]	]	PUNCT
njb-38	25	27	.	.	PUNCT
njb-38	26	1	since	since	SCONJ
njb-38	26	2	,	,	PUNCT
njb-38	26	3	antimonial	antimonial	ADJ
njb-38	26	4	drug	drug	NOUN
njb-38	26	5	resistance	resistance	NOUN
njb-38	26	6	is	be	AUX
njb-38	26	7	becoming	become	VERB
njb-38	26	8	a	a	DET
njb-38	26	9	common	common	ADJ
njb-38	26	10	problem	problem	NOUN
njb-38	26	11	in	in	ADP
njb-38	26	12	many	many	ADJ
njb-38	26	13	leishmaniasis	leishmaniasis	NOUN
njb-38	26	14	endemic	endemic	ADJ
njb-38	26	15	regions	region	NOUN
njb-38	26	16	,	,	PUNCT
njb-38	26	17	extensive	extensive	ADJ
njb-38	26	18	studies	study	NOUN
njb-38	26	19	have	have	AUX
njb-38	26	20	been	be	AUX
njb-38	26	21	carried	carry	VERB
njb-38	26	22	out	out	ADP
njb-38	26	23	to	to	PART
njb-38	26	24	investigate	investigate	VERB
njb-38	26	25	the	the	DET
njb-38	26	26	mechanism	mechanism	NOUN
njb-38	26	27	of	of	ADP
njb-38	26	28	drug	drug	NOUN
njb-38	26	29	resistance	resistance	NOUN
njb-38	26	30	in	in	ADP
njb-38	26	31	the	the	DET
njb-38	26	32	laboratory	laboratory	NOUN
njb-38	26	33	mutants	mutant	NOUN
njb-38	26	34	.	.	PUNCT
njb-38	27	1	one	one	NUM
njb-38	27	2	of	of	ADP
njb-38	27	3	the	the	DET
njb-38	27	4	mechanisms	mechanism	NOUN
njb-38	27	5	of	of	ADP
njb-38	27	6	resistance	resistance	NOUN
njb-38	27	7	is	be	AUX
njb-38	27	8	postulated	postulate	VERB
njb-38	27	9	to	to	PART
njb-38	27	10	be	be	AUX
njb-38	27	11	hsp70	hsp70	PROPN
njb-38	27	12	.	.	PUNCT
njb-38	28	1	l.	l.	PROPN
njb-38	28	2	tarentolae	tarentolae	PROPN
njb-38	28	3	cells	cell	NOUN
njb-38	28	4	transfected	transfecte	VERB
njb-38	28	5	with	with	ADP
njb-38	28	6	hsp70	hsp70	PROPN
njb-38	28	7	gene	gene	NOUN
njb-38	28	8	showed	show	VERB
njb-38	28	9	resistance	resistance	NOUN
njb-38	28	10	to	to	ADP
njb-38	28	11	sbiii	sbiii	NOUN
njb-38	28	12	or	or	CCONJ
njb-38	28	13	arsenite	arsenite	NOUN
njb-38	28	14	[	[	X
njb-38	28	15	10	10	NUM
njb-38	28	16	]	]	PUNCT
njb-38	28	17	.	.	PUNCT
njb-38	29	1	this	this	PRON
njb-38	29	2	suggests	suggest	VERB
njb-38	29	3	that	that	SCONJ
njb-38	29	4	hsp70	hsp70	PROPN
njb-38	29	5	has	have	VERB
njb-38	29	6	a	a	DET
njb-38	29	7	role	role	NOUN
njb-38	29	8	in	in	ADP
njb-38	29	9	antimony	antimony	ADJ
njb-38	29	10	resistance	resistance	NOUN
njb-38	29	11	mechanism	mechanism	NOUN
njb-38	29	12	.	.	PUNCT
njb-38	30	1	in	in	ADP
njb-38	30	2	the	the	DET
njb-38	30	3	present	present	ADJ
njb-38	30	4	study	study	NOUN
njb-38	30	5	,	,	PUNCT
njb-38	30	6	we	we	PRON
njb-38	30	7	have	have	AUX
njb-38	30	8	characterized	characterize	VERB
njb-38	30	9	the	the	DET
njb-38	30	10	expression	expression	NOUN
njb-38	30	11	of	of	ADP
njb-38	30	12	heat	heat	NOUN
njb-38	30	13	shock	shock	NOUN
njb-38	30	14	protein	protein	NOUN
njb-38	30	15	70	70	NUM
njb-38	30	16	in	in	ADP
njb-38	30	17	antimony	antimony	NOUN
njb-38	30	18	sensitive	sensitive	ADJ
njb-38	30	19	and	and	CCONJ
njb-38	30	20	resistant	resistant	ADJ
njb-38	30	21	clinical	clinical	ADJ
njb-38	30	22	isolates	isolate	NOUN
njb-38	30	23	of	of	ADP
njb-38	30	24	l.	l.	PROPN
njb-38	30	25	donovani	donovani	PROPN
njb-38	30	26	.	.	PUNCT
njb-38	31	1	materials	material	NOUN
njb-38	31	2	and	and	CCONJ
njb-38	31	3	methods	method	NOUN
njb-38	31	4	parasite	parasite	NOUN
njb-38	31	5	and	and	CCONJ
njb-38	31	6	culture	culture	NOUN
njb-38	31	7	condition	condition	NOUN
njb-38	31	8	promastigotes	promastigote	NOUN
njb-38	31	9	of	of	ADP
njb-38	31	10	indian	indian	PROPN
njb-38	31	11	leishmania	leishmania	PROPN
njb-38	31	12	donovani	donovani	PROPN
njb-38	31	13	clones	clones	PROPN
njb-38	31	14	ag83	ag83	PROPN
njb-38	31	15	-	-	PUNCT
njb-38	31	16	s	s	NOUN
njb-38	31	17	(	(	PUNCT
njb-38	31	18	mhom	mhom	PROPN
njb-38	31	19	/	/	SYM
njb-38	31	20	in/1983	in/1983	PROPN
njb-38	31	21	/	/	SYM
njb-38	31	22	ag83	ag83	PROPN
njb-38	31	23	)	)	PUNCT
njb-38	31	24	,	,	PUNCT
njb-38	31	25	ge1	ge1	PROPN
njb-38	31	26	(	(	PUNCT
njb-38	31	27	mhom	mhom	PROPN
njb-38	31	28	/	/	SYM
njb-38	31	29	in	in	ADP
njb-38	31	30	/80/	/80/	PROPN
njb-38	31	31	ge1f8r	ge1f8r	PROPN
njb-38	31	32	)	)	PUNCT
njb-38	31	33	and	and	CCONJ
njb-38	31	34	three	three	NUM
njb-38	31	35	untyped	untype	VERB
njb-38	31	36	strains	strain	NOUN
njb-38	31	37	2001	2001	NUM
njb-38	31	38	,	,	PUNCT
njb-38	31	39	ns2	ns2	NOUN
njb-38	31	40	,	,	PUNCT
njb-38	31	41	and	and	CCONJ
njb-38	31	42	41	41	NUM
njb-38	31	43	were	be	AUX
njb-38	31	44	isolated	isolate	VERB
njb-38	31	45	from	from	ADP
njb-38	31	46	patients	patient	NOUN
njb-38	31	47	with	with	ADP
njb-38	31	48	visceral	visceral	NOUN
njb-38	31	49	leishmaniasis	leishmaniasis	NOUN
njb-38	31	50	(	(	PUNCT
njb-38	31	51	vl	vl	NOUN
njb-38	31	52	)	)	PUNCT
njb-38	31	53	and	and	CCONJ
njb-38	31	54	were	be	AUX
njb-38	31	55	routinely	routinely	ADV
njb-38	31	56	cultured	cultured	ADJ
njb-38	31	57	at	at	ADP
njb-38	31	58	22c	22c	NUM
njb-38	31	59	in	in	ADP
njb-38	31	60	m-199	m-199	PROPN
njb-38	31	61	medium	medium	NOUN
njb-38	31	62	(	(	PUNCT
njb-38	31	63	sigma	sigma	PROPN
njb-38	31	64	,	,	PUNCT
njb-38	31	65	usa	usa	PROPN
njb-38	31	66	)	)	PUNCT
njb-38	31	67	supplemented	supplement	VERB
njb-38	31	68	with	with	ADP
njb-38	31	69	10	10	NUM
njb-38	31	70	%	%	NOUN
njb-38	31	71	heat	heat	NOUN
njb-38	31	72	inactivated	inactivate	VERB
njb-38	31	73	fetal	fetal	ADJ
njb-38	31	74	bovine	bovine	NOUN
njb-38	31	75	serum	serum	NOUN
njb-38	31	76	(	(	PUNCT
njb-38	31	77	fbs	fbs	ADJ
njb-38	31	78	,	,	PUNCT
njb-38	31	79	gibco	gibco	PROPN
njb-38	31	80	/	/	SYM
njb-38	31	81	brl	brl	PROPN
njb-38	31	82	,	,	PUNCT
njb-38	31	83	life	life	NOUN
njb-38	31	84	technologies	technology	NOUN
njb-38	31	85	,	,	PUNCT
njb-38	31	86	scotland	scotland	PROPN
njb-38	31	87	,	,	PUNCT
njb-38	31	88	uk	uk	PROPN
njb-38	31	89	)	)	PUNCT
njb-38	31	90	and	and	CCONJ
njb-38	31	91	13	13	NUM
njb-38	31	92	mg	mg	PROPN
njb-38	31	93	/	/	SYM
njb-38	31	94	ml	ml	PROPN
njb-38	31	95	penicillin	penicillin	PROPN
njb-38	31	96	and	and	CCONJ
njb-38	31	97	streptomycin	streptomycin	PROPN
njb-38	31	98	(	(	PUNCT
njb-38	31	99	sigma	sigma	PROPN
njb-38	31	100	,	,	PUNCT
njb-38	31	101	usa	usa	PROPN
njb-38	31	102	)	)	PUNCT
njb-38	32	1	[	[	PUNCT
njb-38	32	2	11	11	NUM
njb-38	32	3	]	]	PUNCT
njb-38	32	4	.	.	PUNCT
njb-38	32	5	nepal	nepal	ADJ
njb-38	32	6	journal	journal	PROPN
njb-38	32	7	of	of	ADP
njb-38	32	8	biotechnology	biotechnology	NOUN
njb-38	32	9	.	.	PUNCT
njb-38	33	1	dec	dec	PROPN
njb-38	33	2	.	.	PROPN
njb-38	33	3	2015	2015	NUM
njb-38	33	4	vol	vol	NOUN
njb-38	33	5	.	.	PROPN
njb-38	34	1	3	3	NUM
njb-38	34	2	,	,	PUNCT
njb-38	34	3	no	no	INTJ
njb-38	34	4	.	.	PUNCT
njb-38	35	1	1:22	1:22	NUM
njb-38	35	2	-	-	SYM
njb-38	35	3	28	28	NUM
njb-38	35	4	maharjan	maharjan	NOUN
njb-38	35	5	and	and	CCONJ
njb-38	35	6	madhubala	madhubala	PROPN
njb-38	35	7	©	©	PROPN
njb-38	35	8	njb	njb	PROPN
njb-38	35	9	,	,	PUNCT
njb-38	35	10	biotechnology	biotechnology	NOUN
njb-38	35	11	society	society	NOUN
njb-38	35	12	of	of	ADP
njb-38	35	13	nepal	nepal	ADJ
njb-38	35	14	23	23	NUM
njb-38	35	15	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-38	35	16	clinical	clinical	ADJ
njb-38	35	17	isolates	isolate	NOUN
njb-38	35	18	obtained	obtain	VERB
njb-38	35	19	from	from	ADP
njb-38	35	20	vl	vl	PROPN
njb-38	35	21	patients	patient	NOUN
njb-38	35	22	who	who	PRON
njb-38	35	23	responded	respond	VERB
njb-38	35	24	to	to	ADP
njb-38	35	25	sag	sag	ADJ
njb-38	35	26	chemotherapy	chemotherapy	NOUN
njb-38	35	27	were	be	AUX
njb-38	35	28	designated	designate	VERB
njb-38	35	29	as	as	ADP
njb-38	35	30	sag	sag	PROPN
njb-38	35	31	-	-	PUNCT
njb-38	35	32	s	s	NOUN
njb-38	35	33	(	(	PUNCT
njb-38	35	34	sag	sag	ADJ
njb-38	35	35	sensitive	sensitive	ADJ
njb-38	35	36	)	)	PUNCT
njb-38	35	37	whereas	whereas	SCONJ
njb-38	35	38	vl	vl	PROPN
njb-38	35	39	patients	patient	NOUN
njb-38	35	40	that	that	PRON
njb-38	35	41	did	do	AUX
njb-38	35	42	not	not	PART
njb-38	35	43	respond	respond	VERB
njb-38	35	44	to	to	ADP
njb-38	35	45	sag	sag	PROPN
njb-38	35	46	were	be	AUX
njb-38	35	47	designated	designate	VERB
njb-38	35	48	as	as	ADP
njb-38	35	49	sag	sag	ADJ
njb-38	35	50	-	-	PUNCT
njb-38	35	51	r	r	NOUN
njb-38	35	52	(	(	PUNCT
njb-38	35	53	sag	sag	ADJ
njb-38	35	54	resistant	resistant	ADJ
njb-38	35	55	)	)	PUNCT
njb-38	35	56	.	.	PUNCT
njb-38	36	1	accordingly	accordingly	ADV
njb-38	36	2	,	,	PUNCT
njb-38	36	3	sag	sag	PROPN
njb-38	36	4	sensitive	sensitive	ADJ
njb-38	36	5	isolate	isolate	VERB
njb-38	36	6	used	use	VERB
njb-38	36	7	in	in	ADP
njb-38	36	8	this	this	DET
njb-38	36	9	study	study	NOUN
njb-38	36	10	is	be	AUX
njb-38	36	11	2001	2001	NUM
njb-38	36	12	-	-	PUNCT
njb-38	36	13	s	s	NOUN
njb-38	36	14	whereas	whereas	SCONJ
njb-38	36	15	the	the	DET
njb-38	36	16	four	four	NUM
njb-38	36	17	sag	sag	ADJ
njb-38	36	18	-	-	PUNCT
njb-38	36	19	r	r	NOUN
njb-38	36	20	isolates	isolate	NOUN
njb-38	36	21	are	be	AUX
njb-38	36	22	41	41	NUM
njb-38	36	23	-	-	PUNCT
njb-38	36	24	r	r	NOUN
njb-38	36	25	,	,	PUNCT
njb-38	36	26	ns2	ns2	PROPN
njb-38	36	27	-	-	PUNCT
njb-38	36	28	r	r	NOUN
njb-38	36	29	,	,	PUNCT
njb-38	36	30	and	and	CCONJ
njb-38	36	31	ge1	ge1	PROPN
njb-38	36	32	-	-	PUNCT
njb-38	36	33	r.	r.	PROPN
njb-38	36	34	the	the	DET
njb-38	36	35	sag	sag	ADJ
njb-38	36	36	-	-	PUNCT
njb-38	36	37	resistant	resistant	ADJ
njb-38	36	38	isolates	isolate	NOUN
njb-38	36	39	were	be	AUX
njb-38	36	40	maintained	maintain	VERB
njb-38	36	41	in	in	ADP
njb-38	36	42	the	the	DET
njb-38	36	43	absence	absence	NOUN
njb-38	36	44	of	of	ADP
njb-38	36	45	drug	drug	NOUN
njb-38	36	46	pressure	pressure	NOUN
njb-38	36	47	in	in	ADP
njb-38	36	48	vitro	vitro	PROPN
njb-38	36	49	.	.	PUNCT
njb-38	37	1	the	the	DET
njb-38	37	2	isolates	isolate	NOUN
njb-38	37	3	have	have	AUX
njb-38	37	4	been	be	AUX
njb-38	37	5	passage	passage	NOUN
njb-38	37	6	through	through	ADP
njb-38	37	7	hamsters	hamster	NOUN
njb-38	37	8	or	or	CCONJ
njb-38	37	9	balb	balb	PROPN
njb-38	37	10	/	/	SYM
njb-38	37	11	c	c	NOUN
njb-38	37	12	mice	mouse	NOUN
njb-38	37	13	to	to	PART
njb-38	37	14	retain	retain	VERB
njb-38	37	15	their	their	PRON
njb-38	37	16	virulence	virulence	NOUN
njb-38	37	17	and	and	CCONJ
njb-38	37	18	importantly	importantly	ADV
njb-38	37	19	their	their	PRON
njb-38	37	20	chemo	chemo	NOUN
njb-38	37	21	-	-	PUNCT
njb-38	37	22	sensitivity	sensitivity	NOUN
njb-38	37	23	profiles	profile	NOUN
njb-38	37	24	have	have	AUX
njb-38	37	25	remained	remain	VERB
njb-38	37	26	unchanged	unchanged	ADJ
njb-38	37	27	.	.	PUNCT
njb-38	38	1	cloning	cloning	NOUN
njb-38	38	2	of	of	ADP
njb-38	38	3	heat	heat	NOUN
njb-38	38	4	shock	shock	NOUN
njb-38	38	5	protein	protein	NOUN
njb-38	38	6	70	70	NUM
njb-38	38	7	(	(	PUNCT
njb-38	38	8	hsp70	hsp70	PROPN
njb-38	38	9	)	)	PUNCT
njb-38	38	10	gene	gene	NOUN
njb-38	38	11	from	from	ADP
njb-38	38	12	l.	l.	PROPN
njb-38	38	13	donovani	donovani	PROPN
njb-38	38	14	.	.	PUNCT
njb-38	39	1	a	a	DET
njb-38	39	2	1962	1962	NUM
njb-38	39	3	-	-	PUNCT
njb-38	39	4	bp	bp	PROPN
njb-38	39	5	dna	dna	PROPN
njb-38	39	6	fragment	fragment	NOUN
njb-38	39	7	was	be	AUX
njb-38	39	8	amplified	amplify	VERB
njb-38	39	9	from	from	ADP
njb-38	39	10	the	the	DET
njb-38	39	11	genomic	genomic	ADJ
njb-38	39	12	dna	dna	NOUN
njb-38	39	13	using	use	VERB
njb-38	39	14	a	a	DET
njb-38	39	15	sense	sense	NOUN
njb-38	39	16	primer	primer	NOUN
njb-38	39	17	with	with	ADP
njb-38	39	18	a	a	DET
njb-38	39	19	flanking	flanking	NOUN
njb-38	39	20	bamhi	bamhi	ADJ
njb-38	39	21	site	site	NOUN
njb-38	39	22	,	,	PUNCT
njb-38	39	23	5’cgcggatccatgacattcgacggcgccatc-3	5’cgcggatccatgacattcgacggcgccatc-3	PROPN
njb-38	39	24	’	'	PUNCT
njb-38	39	25	,	,	PUNCT
njb-38	39	26	at	at	ADP
njb-38	39	27	position	position	NOUN
njb-38	39	28	1	1	NUM
njb-38	39	29	-	-	SYM
njb-38	39	30	21	21	NUM
njb-38	39	31	,	,	PUNCT
njb-38	39	32	and	and	CCONJ
njb-38	39	33	the	the	DET
njb-38	39	34	antisense	antisense	NOUN
njb-38	39	35	primer	primer	NOUN
njb-38	39	36	with	with	ADP
njb-38	39	37	a	a	DET
njb-38	39	38	flanking	flank	VERB
njb-38	39	39	hindiii	hindiii	NOUN
njb-38	39	40	site	site	NOUN
njb-38	39	41	,	,	PUNCT
njb-38	39	42	5	5	NUM
njb-38	39	43	’	'	PUNCT
njb-38	39	44	–	–	PUNCT
njb-38	39	45	cccaagcttttagtcgacctcctcgaccttgg	cccaagcttttagtcgacctcctcgaccttgg	NOUN
njb-38	39	46	–	–	PUNCT
njb-38	39	47	3	3	NUM
njb-38	39	48	’	'	PUNCT
njb-38	39	49	,	,	PUNCT
njb-38	39	50	including	include	VERB
njb-38	39	51	the	the	DET
njb-38	39	52	stop	stop	NOUN
njb-38	39	53	codon	codon	PROPN
njb-38	39	54	,	,	PUNCT
njb-38	39	55	at	at	ADP
njb-38	39	56	position	position	NOUN
njb-38	39	57	1939	1939	NUM
njb-38	39	58	-	-	SYM
njb-38	39	59	1962	1962	NUM
njb-38	39	60	.	.	PUNCT
njb-38	40	1	polymerase	polymerase	NOUN
njb-38	40	2	chain	chain	NOUN
njb-38	40	3	reaction	reaction	NOUN
njb-38	40	4	(	(	PUNCT
njb-38	40	5	pcr	pcr	PROPN
njb-38	40	6	)	)	PUNCT
njb-38	40	7	was	be	AUX
njb-38	40	8	performed	perform	VERB
njb-38	40	9	in	in	ADP
njb-38	40	10	50	50	NUM
njb-38	40	11	µl	µl	NOUN
njb-38	40	12	reaction	reaction	NOUN
njb-38	40	13	volume	volume	NOUN
njb-38	40	14	containing	contain	VERB
njb-38	40	15	100	100	NUM
njb-38	40	16	ng	ng	NOUN
njb-38	40	17	of	of	ADP
njb-38	40	18	genomic	genomic	PROPN
njb-38	40	19	dna	dna	PROPN
njb-38	40	20	,	,	PUNCT
njb-38	40	21	25	25	NUM
njb-38	40	22	pmol	pmol	NOUN
njb-38	40	23	each	each	PRON
njb-38	40	24	of	of	ADP
njb-38	40	25	gene	gene	NOUN
njb-38	40	26	-	-	PUNCT
njb-38	40	27	specific	specific	ADJ
njb-38	40	28	forward	forward	ADV
njb-38	40	29	and	and	CCONJ
njb-38	40	30	reverse	reverse	VERB
njb-38	40	31	primers	primer	NOUN
njb-38	40	32	,	,	PUNCT
njb-38	40	33	200	200	NUM
njb-38	40	34	µmol	µmol	NOUN
njb-38	40	35	of	of	ADP
njb-38	40	36	each	each	DET
njb-38	40	37	dntp	dntp	NOUN
njb-38	40	38	,	,	PUNCT
njb-38	40	39	2	2	NUM
njb-38	40	40	mm	mm	NUM
njb-38	40	41	mgcl2	mgcl2	PROPN
njb-38	40	42	,	,	PUNCT
njb-38	40	43	5u	5u	NUM
njb-38	40	44	taq	taq	NOUN
njb-38	40	45	dna	dna	PROPN
njb-38	40	46	polymerase	polymerase	NOUN
njb-38	40	47	(	(	PUNCT
njb-38	40	48	mbi	mbi	PROPN
njb-38	40	49	fermentas	fermenta	NOUN
njb-38	40	50	)	)	PUNCT
njb-38	40	51	and	and	CCONJ
njb-38	40	52	5	5	NUM
njb-38	40	53	%	%	NOUN
njb-38	40	54	dmso	dmso	NOUN
njb-38	40	55	.	.	PUNCT
njb-38	41	1	the	the	DET
njb-38	41	2	pcr	pcr	PROPN
njb-38	41	3	conditions	condition	NOUN
njb-38	41	4	were	be	AUX
njb-38	41	5	as	as	SCONJ
njb-38	41	6	follows	follow	VERB
njb-38	41	7	:	:	PUNCT
njb-38	41	8	940c	940c	PROPN
njb-38	41	9	for	for	ADP
njb-38	41	10	10	10	NUM
njb-38	41	11	min	min	NOUN
njb-38	41	12	,	,	PUNCT
njb-38	41	13	940	940	NUM
njb-38	41	14	c	c	NOUN
njb-38	41	15	for	for	ADP
njb-38	41	16	45	45	NUM
njb-38	41	17	sec	sec	PROPN
njb-38	41	18	,	,	PUNCT
njb-38	41	19	620c	620c	NUM
njb-38	41	20	for	for	ADP
njb-38	41	21	30	30	NUM
njb-38	41	22	seconds	second	NOUN
njb-38	41	23	,	,	PUNCT
njb-38	41	24	720c	720c	NUM
njb-38	41	25	for	for	ADP
njb-38	41	26	2	2	NUM
njb-38	41	27	min	min	NOUN
njb-38	41	28	and	and	CCONJ
njb-38	41	29	35	35	NUM
njb-38	41	30	cycles	cycle	NOUN
njb-38	41	31	.	.	PUNCT
njb-38	42	1	final	final	ADJ
njb-38	42	2	extension	extension	NOUN
njb-38	42	3	was	be	AUX
njb-38	42	4	carried	carry	VERB
njb-38	42	5	for	for	ADP
njb-38	42	6	10	10	NUM
njb-38	42	7	min	min	NOUN
njb-38	42	8	at	at	ADP
njb-38	42	9	720	720	NUM
njb-38	42	10	.	.	PUNCT
njb-38	43	1	a	a	DET
njb-38	43	2	single	single	ADJ
njb-38	43	3	1.9	1.9	NUM
njb-38	43	4	kb	kb	NOUN
njb-38	43	5	pcr	pcr	PROPN
njb-38	43	6	product	product	NOUN
njb-38	43	7	was	be	AUX
njb-38	43	8	obtained	obtain	VERB
njb-38	43	9	and	and	CCONJ
njb-38	43	10	cloned	clone	VERB
njb-38	43	11	into	into	ADP
njb-38	43	12	the	the	DET
njb-38	43	13	bamhi	bamhi	ADJ
njb-38	43	14	hindiii	hindiii	PROPN
njb-38	43	15	site	site	NOUN
njb-38	43	16	of	of	ADP
njb-38	43	17	pet-30a	pet-30a	NUM
njb-38	43	18	(	(	PUNCT
njb-38	43	19	figure	figure	NOUN
njb-38	43	20	1	1	NUM
njb-38	43	21	)	)	PUNCT
njb-38	43	22	vector	vector	NOUN
njb-38	43	23	(	(	PUNCT
njb-38	43	24	novagen	novagen	PROPN
njb-38	43	25	)	)	PUNCT
njb-38	43	26	.	.	PUNCT
njb-38	44	1	the	the	DET
njb-38	44	2	recombinant	recombinant	ADJ
njb-38	44	3	construct	construct	NOUN
njb-38	44	4	was	be	AUX
njb-38	44	5	transformed	transform	VERB
njb-38	44	6	into	into	ADP
njb-38	44	7	bl21	bl21	PROPN
njb-38	44	8	(	(	PUNCT
njb-38	44	9	de3	de3	PROPN
njb-38	44	10	)	)	PUNCT
njb-38	44	11	strain	strain	NOUN
njb-38	44	12	(	(	PUNCT
njb-38	44	13	figure	figure	NOUN
njb-38	44	14	2	2	NUM
njb-38	44	15	)	)	PUNCT
njb-38	44	16	of	of	ADP
njb-38	44	17	escherichia	escherichia	NOUN
njb-38	44	18	coli	coli	NOUN
njb-38	44	19	and	and	CCONJ
njb-38	44	20	subjected	subject	VERB
njb-38	44	21	to	to	ADP
njb-38	44	22	automated	automate	VERB
njb-38	44	23	sequencing	sequence	VERB
njb-38	44	24	..	..	PUNCT
njb-38	44	25	expression	expression	NOUN
njb-38	44	26	and	and	CCONJ
njb-38	44	27	purification	purification	NOUN
njb-38	44	28	of	of	ADP
njb-38	44	29	hsp70	hsp70	PROPN
njb-38	44	30	protein	protein	NOUN
njb-38	44	31	.	.	PUNCT
njb-38	45	1	expression	expression	NOUN
njb-38	45	2	of	of	ADP
njb-38	45	3	hsp70	hsp70	PROPN
njb-38	45	4	protein	protein	NOUN
njb-38	45	5	from	from	ADP
njb-38	45	6	the	the	DET
njb-38	45	7	construct	construct	NOUN
njb-38	45	8	pet30a	pet30a	ADJ
njb-38	45	9	ldhsp70	ldhsp70	NOUN
njb-38	45	10	was	be	AUX
njb-38	45	11	induced	induce	VERB
njb-38	45	12	at	at	ADP
njb-38	45	13	od	od	ADP
njb-38	45	14	of	of	ADP
njb-38	45	15	0.7	0.7	NUM
njb-38	45	16	with	with	ADP
njb-38	45	17	50	50	NUM
njb-38	45	18	µm	µm	NOUN
njb-38	45	19	,	,	PUNCT
njb-38	45	20	100	100	NUM
njb-38	45	21	µm	µm	NOUN
njb-38	45	22	and	and	CCONJ
njb-38	45	23	500	500	NUM
njb-38	45	24	µm	µm	NOUN
njb-38	45	25	isopropylthiogalactoside	isopropylthiogalactoside	ADJ
njb-38	45	26	(	(	PUNCT
njb-38	45	27	iptg	iptg	PROPN
njb-38	45	28	)	)	PUNCT
njb-38	45	29	(	(	PUNCT
njb-38	45	30	sigma	sigma	PROPN
njb-38	45	31	)	)	PUNCT
njb-38	45	32	at	at	ADP
njb-38	45	33	37c	37c	NUM
njb-38	45	34	for	for	ADP
njb-38	45	35	different	different	ADJ
njb-38	45	36	time	time	NOUN
njb-38	45	37	periods	period	NOUN
njb-38	45	38	.	.	PUNCT
njb-38	46	1	all	all	DET
njb-38	46	2	the	the	DET
njb-38	46	3	steps	step	NOUN
njb-38	46	4	of	of	ADP
njb-38	46	5	purification	purification	NOUN
njb-38	46	6	were	be	AUX
njb-38	46	7	carried	carry	VERB
njb-38	46	8	out	out	ADP
njb-38	46	9	at	at	ADP
njb-38	46	10	4c	4c	NOUN
njb-38	46	11	as	as	SCONJ
njb-38	46	12	mentioned	mention	VERB
njb-38	46	13	in	in	ADP
njb-38	46	14	general	general	ADJ
njb-38	46	15	materials	material	NOUN
njb-38	46	16	and	and	CCONJ
njb-38	46	17	methods	method	NOUN
njb-38	46	18	.	.	PUNCT
njb-38	47	1	figure	figure	NOUN
njb-38	47	2	1	1	NUM
njb-38	47	3	:	:	PUNCT
njb-38	47	4	restriction	restriction	NOUN
njb-38	47	5	map	map	NOUN
njb-38	47	6	of	of	ADP
njb-38	47	7	vector	vector	NOUN
njb-38	47	8	pet-30a(+).the	pet-30a(+).the	PROPN
njb-38	47	9	hsp70	hsp70	NOUN
njb-38	47	10	pcr	pcr	NOUN
njb-38	47	11	product	product	NOUN
njb-38	47	12	(	(	PUNCT
njb-38	47	13	1.9	1.9	NUM
njb-38	47	14	kb	kb	NOUN
njb-38	47	15	)	)	PUNCT
njb-38	47	16	was	be	AUX
njb-38	47	17	cloned	clone	VERB
njb-38	47	18	into	into	ADP
njb-38	47	19	the	the	DET
njb-38	47	20	bamhi	bamhi	PROPN
njb-38	47	21	and	and	CCONJ
njb-38	47	22	hindiii	hindiii	ADJ
njb-38	47	23	restriction	restriction	NOUN
njb-38	47	24	site	site	NOUN
njb-38	47	25	of	of	ADP
njb-38	47	26	pet-30a	pet-30a	NUM
njb-38	47	27	vector	vector	NOUN
njb-38	47	28	.	.	PUNCT
njb-38	48	1	figure	figure	NOUN
njb-38	48	2	2	2	NUM
njb-38	48	3	:	:	PUNCT
njb-38	48	4	cloning	cloning	NOUN
njb-38	48	5	strategy	strategy	NOUN
njb-38	48	6	of	of	ADP
njb-38	48	7	heat	heat	NOUN
njb-38	48	8	shock	shock	NOUN
njb-38	48	9	protein	protein	NOUN
njb-38	48	10	(	(	PUNCT
njb-38	48	11	hsp70	hsp70	PROPN
njb-38	48	12	)	)	PUNCT
njb-38	48	13	in	in	ADP
njb-38	48	14	pet-30a	pet-30a	NUM
njb-38	48	15	expression	expression	NOUN
njb-38	48	16	vector	vector	NOUN
njb-38	48	17	protein	protein	NOUN
njb-38	48	18	determination	determination	NOUN
njb-38	48	19	protein	protein	NOUN
njb-38	48	20	concentration	concentration	NOUN
njb-38	48	21	was	be	AUX
njb-38	48	22	determined	determine	VERB
njb-38	48	23	by	by	ADP
njb-38	48	24	bradford	bradford	PROPN
njb-38	48	25	’s	’s	PART
njb-38	48	26	method	method	NOUN
njb-38	48	27	using	use	VERB
njb-38	48	28	bovine	bovine	ADJ
njb-38	48	29	serum	serum	NOUN
njb-38	48	30	albumin	albumin	NOUN
njb-38	48	31	(	(	PUNCT
njb-38	48	32	bsa	bsa	NOUN
njb-38	48	33	)	)	PUNCT
njb-38	48	34	as	as	ADP
njb-38	48	35	standard	standard	ADJ
njb-38	48	36	[	[	X
njb-38	48	37	12	12	NUM
njb-38	48	38	]	]	PUNCT
njb-38	48	39	.	.	PUNCT
njb-38	49	1	antibody	antibody	NOUN
njb-38	49	2	production	production	NOUN
njb-38	49	3	the	the	DET
njb-38	49	4	purified	purified	ADJ
njb-38	49	5	recombinant	recombinant	NOUN
njb-38	49	6	hsp70	hsp70	NOUN
njb-38	49	7	protein	protein	NOUN
njb-38	49	8	(	(	PUNCT
njb-38	49	9	20	20	NUM
njb-38	49	10	µg	µg	NOUN
njb-38	49	11	)	)	PUNCT
njb-38	49	12	was	be	AUX
njb-38	49	13	subcutaneously	subcutaneously	ADV
njb-38	49	14	injected	inject	VERB
njb-38	49	15	in	in	ADP
njb-38	49	16	mice	mouse	NOUN
njb-38	49	17	using	use	VERB
njb-38	49	18	freund	freund	NOUN
njb-38	49	19	’s	’s	PART
njb-38	49	20	complete	complete	ADJ
njb-38	49	21	nepal	nepal	ADJ
njb-38	49	22	journal	journal	NOUN
njb-38	49	23	of	of	ADP
njb-38	49	24	biotechnology	biotechnology	NOUN
njb-38	49	25	.	.	PUNCT
njb-38	50	1	dec	dec	PROPN
njb-38	50	2	.	.	PROPN
njb-38	50	3	2015	2015	NUM
njb-38	50	4	vol	vol	NOUN
njb-38	50	5	.	.	PROPN
njb-38	51	1	3	3	NUM
njb-38	51	2	,	,	PUNCT
njb-38	51	3	no	no	INTJ
njb-38	51	4	.	.	PUNCT
njb-38	52	1	1:22	1:22	NUM
njb-38	52	2	-	-	SYM
njb-38	52	3	28	28	NUM
njb-38	52	4	maharjan	maharjan	NOUN
njb-38	52	5	and	and	CCONJ
njb-38	52	6	madhubala	madhubala	PROPN
njb-38	52	7	©	©	PROPN
njb-38	52	8	njb	njb	PROPN
njb-38	52	9	,	,	PUNCT
njb-38	52	10	biotechnology	biotechnology	NOUN
njb-38	52	11	society	society	NOUN
njb-38	52	12	of	of	ADP
njb-38	52	13	nepal	nepal	ADJ
njb-38	52	14	24	24	NUM
njb-38	52	15	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-38	52	16	adjuvant	adjuvant	NOUN
njb-38	52	17	followed	follow	VERB
njb-38	52	18	by	by	ADP
njb-38	52	19	two	two	NUM
njb-38	52	20	booster	booster	NOUN
njb-38	52	21	doses	dose	NOUN
njb-38	52	22	of	of	ADP
njb-38	52	23	recombinant	recombinant	ADJ
njb-38	52	24	hsp70	hsp70	NOUN
njb-38	52	25	protein	protein	NOUN
njb-38	52	26	(	(	PUNCT
njb-38	52	27	15	15	NUM
njb-38	52	28	µg	µg	NOUN
njb-38	52	29	)	)	PUNCT
njb-38	52	30	with	with	ADP
njb-38	52	31	incomplete	incomplete	ADJ
njb-38	52	32	adjuvant	adjuvant	NOUN
njb-38	52	33	at	at	ADP
njb-38	52	34	twoweek	twoweek	NOUN
njb-38	52	35	intervals	interval	NOUN
njb-38	52	36	to	to	PART
njb-38	52	37	produce	produce	VERB
njb-38	52	38	the	the	DET
njb-38	52	39	polyclonal	polyclonal	ADJ
njb-38	52	40	antibody	antibody	NOUN
njb-38	52	41	against	against	ADP
njb-38	52	42	the	the	DET
njb-38	52	43	recombinant	recombinant	ADJ
njb-38	52	44	hsp70	hsp70	PROPN
njb-38	52	45	protein	protein	NOUN
njb-38	52	46	.	.	PUNCT
njb-38	53	1	the	the	DET
njb-38	53	2	mice	mouse	NOUN
njb-38	53	3	were	be	AUX
njb-38	53	4	bled	bleed	VERB
njb-38	53	5	two	two	NUM
njb-38	53	6	weeks	week	NOUN
njb-38	53	7	after	after	ADP
njb-38	53	8	the	the	DET
njb-38	53	9	second	second	ADJ
njb-38	53	10	booster	booster	NOUN
njb-38	53	11	dose	dose	NOUN
njb-38	53	12	and	and	CCONJ
njb-38	53	13	sera	sera	PROPN
njb-38	53	14	was	be	AUX
njb-38	53	15	collected	collect	VERB
njb-38	53	16	for	for	ADP
njb-38	53	17	western	western	ADJ
njb-38	53	18	blot	blot	NOUN
njb-38	53	19	analysis	analysis	NOUN
njb-38	53	20	.	.	PUNCT
njb-38	54	1	isolation	isolation	NOUN
njb-38	54	2	of	of	ADP
njb-38	54	3	genomic	genomic	ADJ
njb-38	54	4	dna	dna	PROPN
njb-38	54	5	and	and	CCONJ
njb-38	54	6	total	total	ADJ
njb-38	54	7	rna	rna	PROPN
njb-38	54	8	.	.	PUNCT
njb-38	55	1	genomic	genomic	ADJ
njb-38	55	2	dna	dna	PROPN
njb-38	55	3	was	be	AUX
njb-38	55	4	isolated	isolate	VERB
njb-38	55	5	from	from	ADP
njb-38	55	6	~	~	PUNCT
njb-38	55	7	2	2	NUM
njb-38	55	8	x	x	SYM
njb-38	55	9	109	109	NUM
njb-38	55	10	cells	cell	NOUN
njb-38	55	11	from	from	ADP
njb-38	55	12	10	10	NUM
njb-38	55	13	-	-	SYM
njb-38	55	14	15	15	NUM
njb-38	55	15	ml	ml	NOUN
njb-38	55	16	culture	culture	NOUN
njb-38	55	17	(	(	PUNCT
njb-38	55	18	mid	mid	ADJ
njb-38	55	19	log	log	NOUN
njb-38	55	20	phase	phase	NOUN
njb-38	55	21	promastigotes	promastigote	NOUN
njb-38	55	22	)	)	PUNCT
njb-38	55	23	as	as	SCONJ
njb-38	55	24	described	describe	VERB
njb-38	55	25	in	in	ADP
njb-38	55	26	general	general	ADJ
njb-38	55	27	materials	material	NOUN
njb-38	55	28	and	and	CCONJ
njb-38	55	29	methods	method	NOUN
njb-38	55	30	.	.	PUNCT
njb-38	56	1	5	5	NUM
njb-38	56	2	µg	µg	NOUN
njb-38	56	3	of	of	ADP
njb-38	56	4	genomic	genomic	ADJ
njb-38	56	5	dna	dna	PROPN
njb-38	56	6	was	be	AUX
njb-38	56	7	digested	digest	VERB
njb-38	56	8	overnight	overnight	ADV
njb-38	56	9	with	with	ADP
njb-38	56	10	hindiii	hindiii	NOUN
njb-38	56	11	and	and	CCONJ
njb-38	56	12	subjected	subject	VERB
njb-38	56	13	to	to	ADP
njb-38	56	14	electrophoresis	electrophoresis	NOUN
njb-38	56	15	in	in	ADP
njb-38	56	16	0.8	0.8	NUM
njb-38	56	17	%	%	NOUN
njb-38	56	18	agarose	agarose	NOUN
njb-38	56	19	gels	gel	NOUN
njb-38	56	20	.	.	PUNCT
njb-38	57	1	the	the	DET
njb-38	57	2	gel	gel	NOUN
njb-38	57	3	was	be	AUX
njb-38	57	4	run	run	VERB
njb-38	57	5	at	at	ADP
njb-38	57	6	a	a	DET
njb-38	57	7	constant	constant	ADJ
njb-38	57	8	voltage	voltage	NOUN
njb-38	57	9	of	of	ADP
njb-38	57	10	30v	30v	NOUN
njb-38	57	11	overnight	overnight	ADV
njb-38	57	12	.	.	PUNCT
njb-38	58	1	total	total	ADJ
njb-38	58	2	rna	rna	PROPN
njb-38	58	3	was	be	AUX
njb-38	58	4	isolated	isolate	VERB
njb-38	58	5	from	from	ADP
njb-38	58	6	2	2	NUM
njb-38	58	7			NOUN
njb-38	58	8	108	108	NUM
njb-38	58	9	promastigotes	promastigote	NOUN
njb-38	58	10	using	use	VERB
njb-38	58	11	tri	tri	NOUN
njb-38	58	12	reagenttm	reagenttm	NOUN
njb-38	58	13	(	(	PUNCT
njb-38	58	14	sigma	sigma	PROPN
njb-38	58	15	)	)	PUNCT
njb-38	58	16	according	accord	VERB
njb-38	58	17	to	to	ADP
njb-38	58	18	manufacturer	manufacturer	NOUN
njb-38	58	19	’s	’s	PART
njb-38	58	20	instructions	instruction	NOUN
njb-38	58	21	.	.	PUNCT
njb-38	59	1	the	the	DET
njb-38	59	2	isolated	isolated	ADJ
njb-38	59	3	rna	rna	PROPN
njb-38	59	4	was	be	AUX
njb-38	59	5	stored	store	VERB
njb-38	59	6	in	in	ADP
njb-38	59	7	diethyl	diethyl	PROPN
njb-38	59	8	pyrocarbonate	pyrocarbonate	NOUN
njb-38	59	9	(	(	PUNCT
njb-38	59	10	depc	depc	PROPN
njb-38	59	11	)	)	PUNCT
njb-38	59	12	treated	treat	VERB
njb-38	59	13	water	water	NOUN
njb-38	59	14	in	in	ADP
njb-38	59	15	small	small	ADJ
njb-38	59	16	aliquots	aliquot	NOUN
njb-38	59	17	at	at	ADP
njb-38	59	18	–	–	PUNCT
njb-38	59	19	800c	800c	NUM
njb-38	59	20	.	.	PUNCT
njb-38	60	1	for	for	ADP
njb-38	60	2	northern	northern	ADJ
njb-38	60	3	blot	blot	NOUN
njb-38	60	4	analysis	analysis	NOUN
njb-38	60	5	,	,	PUNCT
njb-38	60	6	15	15	NUM
njb-38	60	7	g	g	NOUN
njb-38	60	8	of	of	ADP
njb-38	60	9	total	total	ADJ
njb-38	60	10	rna	rna	PROPN
njb-38	60	11	was	be	AUX
njb-38	60	12	fractionated	fractionate	VERB
njb-38	60	13	by	by	ADP
njb-38	60	14	denaturing	denature	VERB
njb-38	60	15	agarose	agarose	NOUN
njb-38	60	16	gel	gel	NOUN
njb-38	60	17	electrophoresis	electrophoresis	NOUN
njb-38	60	18	and	and	CCONJ
njb-38	60	19	transferred	transfer	VERB
njb-38	60	20	to	to	ADP
njb-38	60	21	nylon	nylon	NOUN
njb-38	60	22	membrane	membrane	NOUN
njb-38	60	23	following	follow	VERB
njb-38	60	24	the	the	DET
njb-38	60	25	standard	standard	ADJ
njb-38	60	26	procedure	procedure	NOUN
njb-38	60	27	.	.	PUNCT
njb-38	61	1	pulse	pulse	PROPN
njb-38	61	2	field	field	NOUN
njb-38	61	3	gel	gel	NOUN
njb-38	61	4	electrophoresis	electrophoresis	NOUN
njb-38	61	5	(	(	PUNCT
njb-38	61	6	pfge	pfge	NOUN
njb-38	61	7	)	)	PUNCT
njb-38	61	8	leishmania	leishmania	PROPN
njb-38	61	9	chromosomes	chromosome	NOUN
njb-38	61	10	were	be	AUX
njb-38	61	11	separated	separate	VERB
njb-38	61	12	by	by	ADP
njb-38	61	13	pfge	pfge	PROPN
njb-38	61	14	.	.	PUNCT
njb-38	62	1	low	low	ADJ
njb-38	62	2	melting	melt	VERB
njb-38	62	3	agarose	agarose	NOUN
njb-38	62	4	blocks	block	NOUN
njb-38	62	5	,	,	PUNCT
njb-38	62	6	containing	contain	VERB
njb-38	62	7	embedded	embed	VERB
njb-38	62	8	cells	cell	NOUN
njb-38	62	9	(	(	PUNCT
njb-38	62	10	108	108	NUM
njb-38	62	11	log	log	NOUN
njb-38	62	12	phase	phase	NOUN
njb-38	62	13	promastigotes	promastigote	NOUN
njb-38	62	14	/	/	SYM
njb-38	62	15	ml	ml	PROPN
njb-38	62	16	)	)	PUNCT
njb-38	62	17	were	be	AUX
njb-38	62	18	electrophoresed	electrophorese	VERB
njb-38	62	19	in	in	ADP
njb-38	62	20	a	a	DET
njb-38	62	21	contour	contour	ADJ
njb-38	62	22	clamped	clamp	VERB
njb-38	62	23	homogenous	homogenous	ADJ
njb-38	62	24	electric	electric	ADJ
njb-38	62	25	field	field	NOUN
njb-38	62	26	apparatus	apparatus	NOUN
njb-38	62	27	(	(	PUNCT
njb-38	62	28	chef	chef	PROPN
njb-38	62	29	driii	driii	PROPN
njb-38	62	30	,	,	PUNCT
njb-38	62	31	bio	bio	PROPN
njb-38	62	32	-	-	PROPN
njb-38	62	33	rad	rad	ADJ
njb-38	62	34	)	)	PUNCT
njb-38	62	35	in	in	ADP
njb-38	62	36	0.5	0.5	NUM
njb-38	62	37			NOUN
njb-38	62	38	tbe	tbe	NOUN
njb-38	62	39	with	with	ADP
njb-38	62	40	buffer	buffer	NOUN
njb-38	62	41	circulation	circulation	NOUN
njb-38	62	42	at	at	ADP
njb-38	62	43	a	a	DET
njb-38	62	44	constant	constant	ADJ
njb-38	62	45	temperature	temperature	NOUN
njb-38	62	46	of	of	ADP
njb-38	62	47	14ºc	14ºc	ADJ
njb-38	62	48	.	.	PUNCT
njb-38	63	1	saccharomyces	saccharomyce	NOUN
njb-38	63	2	cerevisiae	cerevisiae	NOUN
njb-38	63	3	chromosomes	chromosome	NOUN
njb-38	63	4	were	be	AUX
njb-38	63	5	used	use	VERB
njb-38	63	6	as	as	ADP
njb-38	63	7	chromosomal	chromosomal	ADJ
njb-38	63	8	size	size	NOUN
njb-38	63	9	markers	marker	NOUN
njb-38	63	10	.	.	PUNCT
njb-38	64	1	pulse	pulse	PROPN
njb-38	64	2	field	field	NOUN
njb-38	64	3	gel	gel	NOUN
njb-38	64	4	electrophoresis	electrophoresis	NOUN
njb-38	64	5	(	(	PUNCT
njb-38	64	6	pfge	pfge	NOUN
njb-38	64	7	)	)	PUNCT
njb-38	64	8	running	run	VERB
njb-38	64	9	conditions	condition	NOUN
njb-38	64	10	were	be	AUX
njb-38	64	11	as	as	SCONJ
njb-38	64	12	follows	follow	VERB
njb-38	64	13	:	:	PUNCT
njb-38	64	14	initial	initial	ADJ
njb-38	64	15	switch	switch	NOUN
njb-38	64	16	time	time	NOUN
njb-38	64	17	,	,	PUNCT
njb-38	64	18	60	60	NUM
njb-38	64	19	s	s	NOUN
njb-38	64	20	;	;	PUNCT
njb-38	64	21	final	final	ADJ
njb-38	64	22	switch	switch	NOUN
njb-38	64	23	time	time	NOUN
njb-38	64	24	,	,	PUNCT
njb-38	64	25	120	120	NUM
njb-38	64	26	s	s	NOUN
njb-38	64	27	;	;	PUNCT
njb-38	64	28	run	run	VERB
njb-38	64	29	time	time	NOUN
njb-38	64	30	,	,	PUNCT
njb-38	64	31	24	24	NUM
njb-38	64	32	h	h	NUM
njb-38	64	33	;	;	PUNCT
njb-38	64	34	current	current	ADJ
njb-38	64	35	6v	6v	NUM
njb-38	64	36	/	/	SYM
njb-38	64	37	cm	cm	NOUN
njb-38	64	38	;	;	PUNCT
njb-38	64	39	including	include	VERB
njb-38	64	40	angle	angle	NOUN
njb-38	64	41	120º.	120º.	NUM
njb-38	65	1	the	the	DET
njb-38	65	2	transfer	transfer	NOUN
njb-38	65	3	of	of	ADP
njb-38	65	4	pfge	pfge	NOUN
njb-38	65	5	separated	separate	VERB
njb-38	65	6	chromosomes	chromosome	NOUN
njb-38	65	7	from	from	ADP
njb-38	65	8	agarose	agarose	NOUN
njb-38	65	9	gels	gel	NOUN
njb-38	65	10	to	to	ADP
njb-38	65	11	nylon	nylon	NOUN
njb-38	65	12	membrane	membrane	NOUN
njb-38	65	13	was	be	AUX
njb-38	65	14	achieved	achieve	VERB
njb-38	65	15	by	by	ADP
njb-38	65	16	capillary	capillary	ADJ
njb-38	65	17	method	method	NOUN
njb-38	65	18	as	as	SCONJ
njb-38	65	19	described	describe	VERB
njb-38	65	20	by	by	ADP
njb-38	65	21	[	[	SYM
njb-38	65	22	13	13	NUM
njb-38	65	23	]	]	PUNCT
njb-38	65	24	.	.	PUNCT
njb-38	66	1	hybridization	hybridization	NOUN
njb-38	66	2	of	of	ADP
njb-38	66	3	northern	northern	ADJ
njb-38	66	4	,	,	PUNCT
njb-38	66	5	southern	southern	ADJ
njb-38	66	6	and	and	CCONJ
njb-38	66	7	pfge	pfge	NOUN
njb-38	66	8	blot	blot	NOUN
njb-38	66	9	pre	pre	NOUN
njb-38	66	10	-	-	NOUN
njb-38	66	11	hybridization	hybridization	NOUN
njb-38	66	12	was	be	AUX
njb-38	66	13	done	do	VERB
njb-38	66	14	at	at	ADP
njb-38	66	15	65	65	NUM
njb-38	66	16	°	°	NOUN
njb-38	66	17	c	c	NOUN
njb-38	66	18	for	for	ADP
njb-38	66	19	4	4	NUM
njb-38	66	20	hours	hour	NOUN
njb-38	66	21	in	in	ADP
njb-38	66	22	a	a	DET
njb-38	66	23	buffer	buffer	NOUN
njb-38	66	24	containing	contain	VERB
njb-38	66	25	0.5	0.5	NUM
njb-38	66	26	m	m	NOUN
njb-38	66	27	sodium	sodium	NOUN
njb-38	66	28	phosphate	phosphate	NOUN
njb-38	66	29	;	;	PUNCT
njb-38	66	30	7	7	NUM
njb-38	66	31	%	%	NOUN
njb-38	66	32	sds	sds	NOUN
njb-38	66	33	;	;	PUNCT
njb-38	66	34	1	1	NUM
njb-38	66	35	mm	mm	PROPN
njb-38	66	36	edta	edta	NOUN
njb-38	66	37	(	(	PUNCT
njb-38	66	38	ph	ph	NOUN
njb-38	66	39	8.0	8.0	NUM
njb-38	66	40	)	)	PUNCT
njb-38	66	41	and	and	CCONJ
njb-38	66	42	100	100	NUM
njb-38	66	43	µg	µg	PROPN
njb-38	66	44	/	/	SYM
njb-38	66	45	ml	ml	PROPN
njb-38	66	46	sheared	shear	VERB
njb-38	66	47	denatured	denature	VERB
njb-38	66	48	salmon	salmon	NOUN
njb-38	66	49	sperm	sperm	NOUN
njb-38	66	50	dna	dna	PROPN
njb-38	66	51	.	.	PUNCT
njb-38	67	1	the	the	DET
njb-38	67	2	blots	blot	NOUN
njb-38	67	3	were	be	AUX
njb-38	67	4	hybridised	hybridise	VERB
njb-38	67	5	with	with	ADP
njb-38	67	6	denatured	denature	VERB
njb-38	67	7	α-[p32]-dctp	α-[p32]-dctp	ADV
njb-38	67	8	-	-	PUNCT
njb-38	67	9	labelled	label	VERB
njb-38	67	10	dna	dna	PROPN
njb-38	67	11	probe	probe	NOUN
njb-38	67	12	(	(	PUNCT
njb-38	67	13	pcr	pcr	PROPN
njb-38	67	14	probe	probe	NOUN
njb-38	67	15	described	describe	VERB
njb-38	67	16	for	for	ADP
njb-38	67	17	the	the	DET
njb-38	67	18	l.	l.	PROPN
njb-38	67	19	donovani	donovani	PROPN
njb-38	67	20	hsp70	hsp70	PROPN
njb-38	67	21	coding	code	VERB
njb-38	67	22	region	region	NOUN
njb-38	67	23	)	)	PUNCT
njb-38	67	24	at	at	ADP
njb-38	67	25	106cpm	106cpm	NUM
njb-38	67	26	/	/	SYM
njb-38	67	27	ml	ml	PROPN
njb-38	67	28	.	.	PUNCT
njb-38	68	1	the	the	DET
njb-38	68	2	probe	probe	NOUN
njb-38	68	3	was	be	AUX
njb-38	68	4	labeled	label	VERB
njb-38	68	5	by	by	ADP
njb-38	68	6	random	random	ADJ
njb-38	68	7	priming	priming	NOUN
njb-38	68	8	(	(	PUNCT
njb-38	68	9	neb	neb	NOUN
njb-38	68	10	blot	blot	NOUN
njb-38	68	11	®	®	NOUN
njb-38	68	12	kit	kit	NOUN
njb-38	68	13	,	,	PUNCT
njb-38	68	14	new	new	PROPN
njb-38	68	15	england	england	PROPN
njb-38	68	16	biolabs	biolabs	PROPN
njb-38	68	17	,	,	PUNCT
njb-38	68	18	inc	inc	PROPN
njb-38	68	19	.	.	PROPN
njb-38	68	20	)	)	PUNCT
njb-38	68	21	.	.	PUNCT
njb-38	69	1	membranes	membrane	NOUN
njb-38	69	2	were	be	AUX
njb-38	69	3	washed	wash	VERB
njb-38	69	4	sequentially	sequentially	ADV
njb-38	69	5	as	as	SCONJ
njb-38	69	6	follows	follow	VERB
njb-38	69	7	:	:	PUNCT
njb-38	69	8	2	2	NUM
njb-38	69	9	ssc	ssc	NOUN
njb-38	69	10	,	,	PUNCT
njb-38	69	11	0.1	0.1	NUM
njb-38	69	12	%	%	NOUN
njb-38	69	13	sds	sds	NOUN
njb-38	69	14	;	;	PUNCT
njb-38	69	15	1	1	NUM
njb-38	69	16	ssc	ssc	NOUN
njb-38	69	17	,	,	PUNCT
njb-38	69	18	0.1	0.1	NUM
njb-38	69	19	%	%	NOUN
njb-38	69	20	sds	sds	NOUN
njb-38	69	21	;	;	PUNCT
njb-38	69	22	0.5	0.5	NUM
njb-38	69	23	ssc	ssc	NOUN
njb-38	69	24	,	,	PUNCT
njb-38	69	25	0.1	0.1	NUM
njb-38	69	26	%	%	NOUN
njb-38	69	27	sds	sds	NOUN
njb-38	69	28	;	;	PUNCT
njb-38	69	29	0.2	0.2	NUM
njb-38	69	30	ssc	ssc	NOUN
njb-38	69	31	,	,	PUNCT
njb-38	69	32	0.1	0.1	NUM
njb-38	69	33	%	%	NOUN
njb-38	69	34	sds	sds	NOUN
njb-38	69	35	for	for	ADP
njb-38	69	36	10	10	NUM
njb-38	69	37	min	min	NOUN
njb-38	69	38	each	each	PRON
njb-38	69	39	at	at	ADP
njb-38	69	40	65	65	NUM
njb-38	69	41	°	°	NOUN
njb-38	69	42	c	c	NOUN
njb-38	69	43	until	until	SCONJ
njb-38	69	44	the	the	DET
njb-38	69	45	non	non	ADJ
njb-38	69	46	-	-	ADJ
njb-38	69	47	specific	specific	ADJ
njb-38	69	48	counts	count	NOUN
njb-38	69	49	had	have	AUX
njb-38	69	50	substantially	substantially	ADV
njb-38	69	51	reduced	reduce	VERB
njb-38	69	52	.	.	PUNCT
njb-38	70	1	membranes	membrane	NOUN
njb-38	70	2	were	be	AUX
njb-38	70	3	air	air	NOUN
njb-38	70	4	-	-	PUNCT
njb-38	70	5	dried	dry	VERB
njb-38	70	6	and	and	CCONJ
njb-38	70	7	exposed	expose	VERB
njb-38	70	8	to	to	ADP
njb-38	70	9	imaging	imaging	NOUN
njb-38	70	10	plate	plate	NOUN
njb-38	70	11	.	.	PUNCT
njb-38	71	1	the	the	DET
njb-38	71	2	image	image	NOUN
njb-38	71	3	was	be	AUX
njb-38	71	4	developed	develop	VERB
njb-38	71	5	by	by	ADP
njb-38	71	6	phosphorimager	phosphorimager	NOUN
njb-38	71	7	(	(	PUNCT
njb-38	71	8	fuji	fuji	PROPN
njb-38	71	9	film	film	PROPN
njb-38	71	10	fla-5000	fla-5000	PROPN
njb-38	71	11	,	,	PUNCT
njb-38	71	12	japan	japan	PROPN
njb-38	71	13	)	)	PUNCT
njb-38	71	14	using	use	VERB
njb-38	71	15	image	image	NOUN
njb-38	71	16	quant	quant	ADJ
njb-38	71	17	software	software	NOUN
njb-38	71	18	.	.	PUNCT
njb-38	72	1	western	western	ADJ
njb-38	72	2	blot	blot	NOUN
njb-38	72	3	analysis	analysis	NOUN
njb-38	72	4	late	late	ADJ
njb-38	72	5	log	log	NOUN
njb-38	72	6	phase	phase	NOUN
njb-38	72	7	promastigotes	promastigote	NOUN
njb-38	72	8	(	(	PUNCT
njb-38	72	9	1	1	NUM
njb-38	72	10			NOUN
njb-38	72	11	108	108	NUM
njb-38	72	12	)	)	PUNCT
njb-38	72	13	were	be	AUX
njb-38	72	14	harvested	harvest	VERB
njb-38	72	15	and	and	CCONJ
njb-38	72	16	the	the	DET
njb-38	72	17	resultant	resultant	NOUN
njb-38	72	18	cell	cell	NOUN
njb-38	72	19	pellet	pellet	NOUN
njb-38	72	20	was	be	AUX
njb-38	72	21	lysed	lyse	VERB
njb-38	72	22	by	by	ADP
njb-38	72	23	sonication	sonication	NOUN
njb-38	72	24	and	and	CCONJ
njb-38	72	25	cell	cell	NOUN
njb-38	72	26	supernatants	supernatant	NOUN
njb-38	72	27	were	be	AUX
njb-38	72	28	prepared	prepare	VERB
njb-38	72	29	by	by	ADP
njb-38	72	30	centrifugation	centrifugation	NOUN
njb-38	72	31	at	at	ADP
njb-38	72	32	20,000	20,000	NUM
njb-38	72	33	x	x	SYM
njb-38	72	34	g.	g.	NOUN
njb-38	72	35	50	50	NUM
njb-38	72	36	µg	µg	NOUN
njb-38	72	37	of	of	ADP
njb-38	72	38	protein	protein	NOUN
njb-38	72	39	from	from	ADP
njb-38	72	40	each	each	DET
njb-38	72	41	cell	cell	NOUN
njb-38	72	42	line	line	NOUN
njb-38	72	43	were	be	AUX
njb-38	72	44	fractionated	fractionate	VERB
njb-38	72	45	by	by	ADP
njb-38	72	46	sds	sds	NOUN
njb-38	72	47	-	-	PUNCT
njb-38	72	48	polyacrylamide	polyacrylamide	NOUN
njb-38	72	49	gel	gel	NOUN
njb-38	72	50	electrophoresis	electrophoresis	NOUN
njb-38	72	51	and	and	CCONJ
njb-38	72	52	blotted	blot	VERB
njb-38	72	53	on	on	ADP
njb-38	72	54	nitrocellulose	nitrocellulose	NOUN
njb-38	72	55	membrane	membrane	NOUN
njb-38	72	56	using	use	VERB
njb-38	72	57	electrophoretic	electrophoretic	ADJ
njb-38	72	58	transfer	transfer	NOUN
njb-38	72	59	cell	cell	NOUN
njb-38	72	60	(	(	PUNCT
njb-38	72	61	biorad	biorad	NOUN
njb-38	72	62	)	)	PUNCT
njb-38	72	63	.	.	PUNCT
njb-38	73	1	western	western	ADJ
njb-38	73	2	blot	blot	NOUN
njb-38	73	3	analysis	analysis	NOUN
njb-38	73	4	was	be	AUX
njb-38	73	5	done	do	VERB
njb-38	73	6	using	use	VERB
njb-38	73	7	the	the	DET
njb-38	73	8	ecl	ecl	PROPN
njb-38	73	9	kit	kit	PROPN
njb-38	73	10	(	(	PUNCT
njb-38	73	11	amersham	amersham	PROPN
njb-38	73	12	pharmacia	pharmacia	PROPN
njb-38	73	13	biotech	biotech	PROPN
njb-38	73	14	)	)	PUNCT
njb-38	73	15	according	accord	VERB
njb-38	73	16	to	to	ADP
njb-38	73	17	the	the	DET
njb-38	73	18	manufacturer	manufacturer	NOUN
njb-38	73	19	’s	’s	PART
njb-38	73	20	protocol	protocol	NOUN
njb-38	73	21	.	.	PUNCT
njb-38	74	1	polyclonal	polyclonal	ADJ
njb-38	74	2	antibody	antibody	NOUN
njb-38	74	3	to	to	PART
njb-38	74	4	purified	purify	VERB
njb-38	74	5	recombinant	recombinant	PROPN
njb-38	74	6	l.	l.	PROPN
njb-38	74	7	donovani	donovani	PROPN
njb-38	74	8	hsp70	hsp70	PROPN
njb-38	74	9	generated	generate	VERB
njb-38	74	10	in	in	ADP
njb-38	74	11	mice	mouse	NOUN
njb-38	74	12	was	be	AUX
njb-38	74	13	used	use	VERB
njb-38	74	14	for	for	ADP
njb-38	74	15	the	the	DET
njb-38	74	16	western	western	ADJ
njb-38	74	17	blot	blot	NOUN
njb-38	74	18	analysis	analysis	NOUN
njb-38	74	19	.	.	PUNCT
njb-38	75	1	autoradiograms	autoradiogram	NOUN
njb-38	75	2	were	be	AUX
njb-38	75	3	analyzed	analyze	VERB
njb-38	75	4	by	by	ADP
njb-38	75	5	using	use	VERB
njb-38	75	6	model	model	NOUN
njb-38	75	7	fla	fla	PROPN
njb-38	75	8	5000	5000	NUM
njb-38	75	9	imaging	imaging	NOUN
njb-38	75	10	densitometer	densitometer	NOUN
njb-38	75	11	(	(	PUNCT
njb-38	75	12	fuji	fuji	PROPN
njb-38	75	13	,	,	PUNCT
njb-38	75	14	japan	japan	PROPN
njb-38	75	15	)	)	PUNCT
njb-38	75	16	.	.	PUNCT
njb-38	76	1	the	the	DET
njb-38	76	2	results	result	NOUN
njb-38	76	3	shown	show	VERB
njb-38	76	4	are	be	AUX
njb-38	76	5	from	from	ADP
njb-38	76	6	a	a	DET
njb-38	76	7	single	single	ADJ
njb-38	76	8	experiment	experiment	NOUN
njb-38	76	9	typical	typical	ADJ
njb-38	76	10	of	of	ADP
njb-38	76	11	at	at	ADV
njb-38	76	12	least	least	ADV
njb-38	76	13	three	three	NUM
njb-38	76	14	giving	give	VERB
njb-38	76	15	identical	identical	ADJ
njb-38	76	16	results	result	NOUN
njb-38	76	17	.	.	PUNCT
njb-38	77	1	results	result	VERB
njb-38	77	2	heat	heat	NOUN
njb-38	77	3	shock	shock	NOUN
njb-38	77	4	protein	protein	NOUN
njb-38	77	5	70	70	NUM
njb-38	77	6	(	(	PUNCT
njb-38	77	7	hsp70	hsp70	PROPN
njb-38	77	8	)	)	PUNCT
njb-38	77	9	sequence	sequence	NOUN
njb-38	77	10	analysis	analysis	NOUN
njb-38	77	11	the	the	DET
njb-38	77	12	gene	gene	NOUN
njb-38	77	13	encoding	encode	VERB
njb-38	77	14	the	the	DET
njb-38	77	15	heat	heat	NOUN
njb-38	77	16	shock	shock	NOUN
njb-38	77	17	protein	protein	NOUN
njb-38	77	18	70	70	NUM
njb-38	77	19	kda	kda	NOUN
njb-38	77	20	(	(	PUNCT
njb-38	77	21	hsp70	hsp70	PROPN
njb-38	77	22	)	)	PUNCT
njb-38	77	23	nucleotide	nucleotide	NOUN
njb-38	77	24	sequence	sequence	NOUN
njb-38	77	25	was	be	AUX
njb-38	77	26	retrieved	retrieve	VERB
njb-38	77	27	from	from	ADP
njb-38	77	28	embl	embl	NOUN
njb-38	77	29	sequence	sequence	NOUN
njb-38	77	30	data	datum	NOUN
njb-38	77	31	bank	bank	NOUN
njb-38	77	32	under	under	ADP
njb-38	77	33	the	the	DET
njb-38	77	34	accession	accession	NOUN
njb-38	77	35	no	no	NOUN
njb-38	77	36	.	.	PUNCT
njb-38	78	1	x52314	x52314	INTJ
njb-38	78	2	.	.	PUNCT
njb-38	79	1	in	in	ADP
njb-38	79	2	order	order	NOUN
njb-38	79	3	to	to	PART
njb-38	79	4	clone	clone	VERB
njb-38	79	5	the	the	DET
njb-38	79	6	full	full	ADJ
njb-38	79	7	length	length	NOUN
njb-38	79	8	gene	gene	NOUN
njb-38	79	9	encoding	encode	VERB
njb-38	79	10	hsp70	hsp70	PROPN
njb-38	79	11	,	,	PUNCT
njb-38	79	12	polymerase	polymerase	NOUN
njb-38	79	13	chain	chain	NOUN
njb-38	79	14	reaction	reaction	NOUN
njb-38	79	15	(	(	PUNCT
njb-38	79	16	pcr	pcr	PROPN
njb-38	79	17	)	)	PUNCT
njb-38	79	18	was	be	AUX
njb-38	79	19	performed	perform	VERB
njb-38	79	20	using	use	VERB
njb-38	79	21	specific	specific	ADJ
njb-38	79	22	oligonucleotides	oligonucleotide	NOUN
njb-38	79	23	.	.	PUNCT
njb-38	80	1	the	the	DET
njb-38	80	2	sense	sense	NOUN
njb-38	80	3	primer	primer	NOUN
njb-38	80	4	was	be	AUX
njb-38	80	5	5'-cgcggatccatg	5'-cgcggatccatg	NUM
njb-38	80	6	acattcgacggcgccatc-3	acattcgacggcgccatc-3	NUM
njb-38	80	7	’	'	PUNCT
njb-38	80	8	at	at	ADP
njb-38	80	9	position	position	NOUN
njb-38	80	10	1	1	NUM
njb-38	80	11	-	-	SYM
njb-38	80	12	21	21	NUM
njb-38	80	13	and	and	CCONJ
njb-38	80	14	the	the	DET
njb-38	80	15	antisense	antisense	NOUN
njb-38	80	16	primer	primer	NOUN
njb-38	80	17	with	with	ADP
njb-38	80	18	a	a	DET
njb-38	80	19	flanking	flank	VERB
njb-38	80	20	hindiii	hindiii	NOUN
njb-38	80	21	site	site	NOUN
njb-38	80	22	,	,	PUNCT
njb-38	80	23	5’cccaagcttttagtcgacctcctcgaccttgg3	5’cccaagcttttagtcgacctcctcgaccttgg3	NUM
njb-38	80	24	’	'	PUNCT
njb-38	80	25	including	include	VERB
njb-38	80	26	the	the	DET
njb-38	80	27	stop	stop	NOUN
njb-38	80	28	codon	codon	NOUN
njb-38	80	29	at	at	ADP
njb-38	80	30	position	position	NOUN
njb-38	80	31	1939	1939	NUM
njb-38	80	32	-	-	SYM
njb-38	80	33	1962	1962	NUM
njb-38	80	34	.	.	PUNCT
njb-38	81	1	genomic	genomic	ADJ
njb-38	81	2	dna	dna	PROPN
njb-38	81	3	from	from	ADP
njb-38	81	4	l.	l.	PROPN
njb-38	81	5	donovani	donovani	PROPN
njb-38	81	6	ag83	ag83	PROPN
njb-38	81	7	(	(	PUNCT
njb-38	81	8	mhom	mhom	PROPN
njb-38	81	9	/	/	SYM
njb-38	81	10	in/1983	in/1983	PROPN
njb-38	81	11	/	/	SYM
njb-38	81	12	ag83	ag83	PROPN
njb-38	81	13	)	)	PUNCT
njb-38	81	14	promastigotes	promastigote	NOUN
njb-38	81	15	was	be	AUX
njb-38	81	16	used	use	VERB
njb-38	81	17	as	as	ADP
njb-38	81	18	a	a	DET
njb-38	81	19	template	template	NOUN
njb-38	81	20	.	.	PUNCT
njb-38	82	1	a	a	DET
njb-38	82	2	single	single	ADJ
njb-38	82	3	full	full	ADJ
njb-38	82	4	length	length	NOUN
njb-38	82	5	1962	1962	NUM
njb-38	82	6	-	-	PUNCT
njb-38	82	7	bp	bp	NOUN
njb-38	82	8	pcr	pcr	PROPN
njb-38	82	9	product	product	NOUN
njb-38	82	10	was	be	AUX
njb-38	82	11	obtained	obtain	VERB
njb-38	82	12	.	.	PUNCT
njb-38	83	1	the	the	DET
njb-38	83	2	pcr	pcr	PROPN
njb-38	83	3	fragment	fragment	NOUN
njb-38	83	4	was	be	AUX
njb-38	83	5	purified	purify	VERB
njb-38	83	6	from	from	ADP
njb-38	83	7	the	the	DET
njb-38	83	8	gel	gel	NOUN
njb-38	83	9	and	and	CCONJ
njb-38	83	10	double	double	ADJ
njb-38	83	11	digested	digest	VERB
njb-38	83	12	with	with	ADP
njb-38	83	13	bamh1	bamh1	ADJ
njb-38	83	14	and	and	CCONJ
njb-38	83	15	hindiii	hindiii	ADJ
njb-38	83	16	restriction	restriction	NOUN
njb-38	83	17	enzymes	enzyme	NOUN
njb-38	83	18	.	.	PUNCT
njb-38	84	1	the	the	DET
njb-38	84	2	digested	digest	VERB
njb-38	84	3	product	product	NOUN
njb-38	84	4	was	be	AUX
njb-38	84	5	ligated	ligate	VERB
njb-38	84	6	with	with	ADP
njb-38	84	7	pet30a	pet30a	PROPN
njb-38	84	8	cloning	cloning	NOUN
njb-38	84	9	vector	vector	NOUN
njb-38	84	10	at	at	ADP
njb-38	84	11	160c	160c	NOUN
njb-38	84	12	overnight	overnight	ADV
njb-38	84	13	.	.	PUNCT
njb-38	85	1	the	the	DET
njb-38	85	2	ligated	ligate	VERB
njb-38	85	3	product	product	NOUN
njb-38	85	4	was	be	AUX
njb-38	85	5	transformed	transform	VERB
njb-38	85	6	into	into	ADP
njb-38	85	7	dh5α	dh5α	PROPN
njb-38	85	8	competent	competent	ADJ
njb-38	85	9	cells	cell	NOUN
njb-38	85	10	.	.	PUNCT
njb-38	86	1	the	the	DET
njb-38	86	2	positive	positive	ADJ
njb-38	86	3	clones	clone	NOUN
njb-38	86	4	were	be	AUX
njb-38	86	5	confirmed	confirm	VERB
njb-38	86	6	by	by	ADP
njb-38	86	7	colony	colony	NOUN
njb-38	86	8	pcr	pcr	PROPN
njb-38	86	9	.	.	PUNCT
njb-38	87	1	further	far	ADV
njb-38	87	2	,	,	PUNCT
njb-38	87	3	the	the	DET
njb-38	87	4	cloned	clone	VERB
njb-38	87	5	hsp70	hsp70	NOUN
njb-38	87	6	pcr	pcr	NOUN
njb-38	87	7	fragment	fragment	ADJ
njb-38	87	8	was	be	AUX
njb-38	87	9	confirmed	confirm	VERB
njb-38	87	10	by	by	ADP
njb-38	87	11	sequencing	sequence	VERB
njb-38	87	12	.	.	PUNCT
njb-38	88	1	a	a	DET
njb-38	88	2	single	single	ADJ
njb-38	88	3	open	open	ADJ
njb-38	88	4	reading	reading	NOUN
njb-38	88	5	frame	frame	NOUN
njb-38	88	6	consisting	consist	VERB
njb-38	88	7	of	of	ADP
njb-38	88	8	1962	1962	NUM
njb-38	88	9	-	-	PUNCT
njb-38	88	10	bp	bp	PROPN
njb-38	88	11	was	be	AUX
njb-38	88	12	isolated	isolate	VERB
njb-38	88	13	.	.	PUNCT
njb-38	89	1	no	no	DET
njb-38	89	2	variation	variation	NOUN
njb-38	89	3	in	in	ADP
njb-38	89	4	the	the	DET
njb-38	89	5	coding	code	VERB
njb-38	89	6	sequence	sequence	NOUN
njb-38	89	7	was	be	AUX
njb-38	89	8	found	find	VERB
njb-38	89	9	from	from	ADP
njb-38	89	10	the	the	DET
njb-38	89	11	sequence	sequence	NOUN
njb-38	89	12	described	describe	VERB
njb-38	89	13	earlier	early	ADV
njb-38	89	14	.	.	PUNCT
njb-38	90	1	nepal	nepal	ADJ
njb-38	90	2	journal	journal	PROPN
njb-38	90	3	of	of	ADP
njb-38	90	4	biotechnology	biotechnology	NOUN
njb-38	90	5	.	.	PUNCT
njb-38	91	1	dec	dec	PROPN
njb-38	91	2	.	.	PROPN
njb-38	91	3	2015	2015	NUM
njb-38	91	4	vol	vol	NOUN
njb-38	91	5	.	.	PROPN
njb-38	92	1	3	3	NUM
njb-38	92	2	,	,	PUNCT
njb-38	92	3	no	no	INTJ
njb-38	92	4	.	.	PUNCT
njb-38	93	1	1:22	1:22	NUM
njb-38	93	2	-	-	SYM
njb-38	93	3	28	28	NUM
njb-38	93	4	maharjan	maharjan	NOUN
njb-38	93	5	and	and	CCONJ
njb-38	93	6	madhubala	madhubala	PROPN
njb-38	93	7	©	©	PROPN
njb-38	93	8	njb	njb	PROPN
njb-38	93	9	,	,	PUNCT
njb-38	93	10	biotechnology	biotechnology	NOUN
njb-38	93	11	society	society	NOUN
njb-38	93	12	of	of	ADP
njb-38	93	13	nepal	nepal	ADJ
njb-38	93	14	25	25	NUM
njb-38	93	15	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-38	93	16	over	over	ADP
njb-38	93	17	-	-	PUNCT
njb-38	93	18	expression	expression	NOUN
njb-38	93	19	and	and	CCONJ
njb-38	93	20	purification	purification	NOUN
njb-38	93	21	of	of	ADP
njb-38	93	22	fulllength	fulllength	PROPN
njb-38	93	23	l.	l.	PROPN
njb-38	93	24	donovani	donovani	PROPN
njb-38	93	25	hsp70	hsp70	PROPN
njb-38	93	26	in	in	ADP
njb-38	93	27	e.	e.	PROPN
njb-38	93	28	coli	coli	PROPN
njb-38	93	29	in	in	ADP
njb-38	93	30	order	order	NOUN
njb-38	93	31	to	to	PART
njb-38	93	32	overexpress	overexpress	NOUN
njb-38	93	33	and	and	CCONJ
njb-38	93	34	purify	purify	VERB
njb-38	93	35	recombinant	recombinant	ADJ
njb-38	93	36	protein	protein	NOUN
njb-38	93	37	,	,	PUNCT
njb-38	93	38	the	the	DET
njb-38	93	39	encoding	encoding	PROPN
njb-38	93	40	l.	l.	PROPN
njb-38	93	41	donovani	donovani	PROPN
njb-38	93	42	hsp70	hsp70	PROPN
njb-38	93	43	sequence	sequence	NOUN
njb-38	93	44	was	be	AUX
njb-38	93	45	cloned	clone	VERB
njb-38	93	46	inframe	inframe	NOUN
njb-38	93	47	in	in	ADP
njb-38	93	48	pet-30a	pet-30a	NUM
njb-38	93	49	expression	expression	NOUN
njb-38	93	50	vector	vector	NOUN
njb-38	93	51	.	.	PUNCT
njb-38	94	1	the	the	DET
njb-38	94	2	resultant	resultant	NOUN
njb-38	94	3	pet-30a	pet-30a	NUM
njb-38	94	4	-ldhsp70	-ldhsp70	PUNCT
njb-38	94	5	construct	construct	NOUN
njb-38	94	6	was	be	AUX
njb-38	94	7	transformed	transform	VERB
njb-38	94	8	into	into	ADP
njb-38	94	9	e.	e.	PROPN
njb-38	94	10	coli	coli	PROPN
njb-38	94	11	and	and	CCONJ
njb-38	94	12	protein	protein	NOUN
njb-38	94	13	over	over	ADP
njb-38	94	14	expression	expression	NOUN
njb-38	94	15	was	be	AUX
njb-38	94	16	induced	induce	VERB
njb-38	94	17	using	use	VERB
njb-38	94	18	iptg	iptg	NOUN
njb-38	94	19	(	(	PUNCT
njb-38	94	20	figure	figure	NOUN
njb-38	94	21	3a	3a	NUM
njb-38	94	22	and	and	CCONJ
njb-38	94	23	3b	3b	NUM
njb-38	94	24	)	)	PUNCT
njb-38	94	25	as	as	SCONJ
njb-38	94	26	described	describe	VERB
njb-38	94	27	in	in	ADP
njb-38	94	28	general	general	ADJ
njb-38	94	29	materials	material	NOUN
njb-38	94	30	and	and	CCONJ
njb-38	94	31	method	method	NOUN
njb-38	94	32	.	.	PUNCT
njb-38	95	1	a	a	DET
njb-38	95	2	protein	protein	NOUN
njb-38	95	3	with	with	ADP
njb-38	95	4	a	a	DET
njb-38	95	5	molecular	molecular	ADJ
njb-38	95	6	weight	weight	NOUN
njb-38	95	7	that	that	PRON
njb-38	95	8	matched	match	VERB
njb-38	95	9	the	the	DET
njb-38	95	10	estimated	estimate	VERB
njb-38	95	11	70	70	NUM
njb-38	95	12	kda	kda	NOUN
njb-38	95	13	according	accord	VERB
njb-38	95	14	to	to	ADP
njb-38	95	15	amino	amino	NOUN
njb-38	95	16	acid	acid	NOUN
njb-38	95	17	composition	composition	NOUN
njb-38	95	18	of	of	ADP
njb-38	95	19	l.	l.	PROPN
njb-38	95	20	donovani	donovani	PROPN
njb-38	95	21	hsp70	hsp70	PROPN
njb-38	95	22	with	with	ADP
njb-38	95	23	his6	his6	PROPN
njb-38	95	24	tag	tag	NOUN
njb-38	95	25	and	and	CCONJ
njb-38	95	26	s	s	NOUN
njb-38	95	27	-	-	NOUN
njb-38	95	28	tag	tag	NOUN
njb-38	95	29	present	present	NOUN
njb-38	95	30	at	at	ADP
njb-38	95	31	its	its	PRON
njb-38	95	32	n	n	ADJ
njb-38	95	33	-	-	ADJ
njb-38	95	34	terminal	terminal	ADJ
njb-38	95	35	end	end	NOUN
njb-38	95	36	was	be	AUX
njb-38	95	37	also	also	ADV
njb-38	95	38	induced	induce	VERB
njb-38	95	39	.	.	PUNCT
njb-38	96	1	the	the	DET
njb-38	96	2	recombinant	recombinant	ADJ
njb-38	96	3	protein	protein	NOUN
njb-38	96	4	was	be	AUX
njb-38	96	5	purified	purify	VERB
njb-38	96	6	by	by	ADP
njb-38	96	7	ni2	ni2	PROPN
njb-38	96	8	+	+	PROPN
njb-38	96	9	-nta	-nta	PROPN
njb-38	96	10	affinity	affinity	NOUN
njb-38	96	11	chromatography	chromatography	NOUN
njb-38	96	12	column	column	NOUN
njb-38	96	13	purification	purification	NOUN
njb-38	96	14	of	of	ADP
njb-38	96	15	his	his	PRON
njb-38	96	16	-	-	PUNCT
njb-38	96	17	tagged	tag	VERB
njb-38	96	18	l.	l.	PROPN
njb-38	96	19	donovani	donovani	X
njb-38	96	20	hsp70	hsp70	NOUN
njb-38	96	21	by	by	ADP
njb-38	96	22	metal	metal	NOUN
njb-38	96	23	affinity	affinity	NOUN
njb-38	96	24	chromatography	chromatography	NOUN
njb-38	96	25	(	(	PUNCT
njb-38	96	26	figure	figure	NOUN
njb-38	96	27	3c	3c	NUM
njb-38	96	28	)	)	PUNCT
njb-38	96	29	yielded	yield	VERB
njb-38	96	30	~200	~200	PRON
njb-38	96	31	µg	µg	NOUN
njb-38	96	32	of	of	ADP
njb-38	96	33	purified	purified	ADJ
njb-38	96	34	protein	protein	NOUN
njb-38	96	35	from	from	ADP
njb-38	96	36	1litre	1litre	NUM
njb-38	96	37	bacterial	bacterial	ADJ
njb-38	96	38	culture	culture	NOUN
njb-38	96	39	.	.	PUNCT
njb-38	97	1	figure	figure	NOUN
njb-38	97	2	3	3	NUM
njb-38	97	3	:	:	PUNCT
njb-38	97	4	overexpression	overexpression	NOUN
njb-38	97	5	and	and	CCONJ
njb-38	97	6	purification	purification	NOUN
njb-38	97	7	of	of	ADP
njb-38	97	8	l.	l.	PROPN
njb-38	97	9	donovani	donovani	PROPN
njb-38	97	10	hsp70	hsp70	PROPN
njb-38	97	11	protein	protein	NOUN
njb-38	97	12	.	.	PUNCT
njb-38	98	1	(	(	PUNCT
njb-38	98	2	a	a	DET
njb-38	98	3	)	)	PUNCT
njb-38	98	4	coomassie	coomassie	ADJ
njb-38	98	5	blue	blue	ADJ
njb-38	98	6	staining	staining	NOUN
njb-38	98	7	of	of	ADP
njb-38	98	8	sds	sds	PROPN
njb-38	98	9	–	–	PUNCT
njb-38	98	10	page	page	NOUN
njb-38	98	11	showing	show	VERB
njb-38	98	12	overexpression	overexpression	NOUN
njb-38	98	13	of	of	ADP
njb-38	98	14	full	full	ADJ
njb-38	98	15	-	-	PUNCT
njb-38	98	16	length	length	NOUN
njb-38	98	17	l.	l.	PROPN
njb-38	98	18	donovani	donovani	PROPN
njb-38	98	19	hsp70	hsp70	PROPN
njb-38	98	20	protein	protein	NOUN
njb-38	98	21	in	in	ADP
njb-38	98	22	e.	e.	PROPN
njb-38	98	23	coli	coli	PROPN
njb-38	98	24	.	.	PUNCT
njb-38	99	1	the	the	DET
njb-38	99	2	pet-30a	pet-30a	NUM
njb-38	99	3	bacterial	bacterial	ADJ
njb-38	99	4	extract	extract	NOUN
njb-38	99	5	before	before	ADP
njb-38	99	6	induction	induction	NOUN
njb-38	99	7	(	(	PUNCT
njb-38	99	8	lane	lane	NOUN
njb-38	99	9	2	2	NUM
njb-38	99	10	and	and	CCONJ
njb-38	99	11	6	6	NUM
njb-38	99	12	)	)	PUNCT
njb-38	99	13	and	and	CCONJ
njb-38	99	14	after	after	ADP
njb-38	99	15	induction	induction	NOUN
njb-38	99	16	(	(	PUNCT
njb-38	99	17	lanes	lane	VERB
njb-38	99	18	3	3	NUM
njb-38	99	19	-	-	SYM
njb-38	99	20	5	5	NUM
njb-38	99	21	and	and	CCONJ
njb-38	99	22	7	7	NUM
njb-38	99	23	-	-	SYM
njb-38	99	24	9	9	NUM
njb-38	99	25	)	)	PUNCT
njb-38	99	26	of	of	ADP
njb-38	99	27	two	two	NUM
njb-38	99	28	positive	positive	ADJ
njb-38	99	29	clones	clone	NOUN
njb-38	99	30	at	at	ADP
njb-38	99	31	1	1	NUM
njb-38	99	32	,	,	PUNCT
njb-38	99	33	2	2	NUM
njb-38	99	34	,	,	PUNCT
njb-38	99	35	and	and	CCONJ
njb-38	99	36	3	3	NUM
njb-38	99	37	h	h	NOUN
njb-38	99	38	,	,	PUNCT
njb-38	99	39	respectively	respectively	ADV
njb-38	99	40	with	with	ADP
njb-38	99	41	1	1	NUM
njb-38	99	42	mm	mm	NOUN
njb-38	99	43	iptg	iptg	NOUN
njb-38	99	44	.	.	PUNCT
njb-38	100	1	arrow	arrow	NOUN
njb-38	100	2	shows	show	VERB
njb-38	100	3	the	the	DET
njb-38	100	4	induced	induced	ADJ
njb-38	100	5	recombinant	recombinant	ADJ
njb-38	100	6	hsp70	hsp70	PROPN
njb-38	100	7	protein	protein	NOUN
njb-38	100	8	.	.	PUNCT
njb-38	101	1	broad	broad	ADJ
njb-38	101	2	range	range	NOUN
njb-38	101	3	protein	protein	NOUN
njb-38	101	4	mw	mw	X
njb-38	101	5	marker	marker	NOUN
njb-38	101	6	(	(	PUNCT
njb-38	101	7	mbifermentas	mbifermenta	NOUN
njb-38	101	8	)	)	PUNCT
njb-38	101	9	was	be	AUX
njb-38	101	10	used	use	VERB
njb-38	101	11	to	to	PART
njb-38	101	12	identify	identify	VERB
njb-38	101	13	the	the	DET
njb-38	101	14	size	size	NOUN
njb-38	101	15	of	of	ADP
njb-38	101	16	the	the	DET
njb-38	101	17	recombinant	recombinant	ADJ
njb-38	101	18	protein	protein	NOUN
njb-38	101	19	(	(	PUNCT
njb-38	101	20	lane	lane	NOUN
njb-38	101	21	1	1	NUM
njb-38	101	22	)	)	PUNCT
njb-38	101	23	.	.	PUNCT
njb-38	102	1	(	(	PUNCT
njb-38	102	2	b	b	X
njb-38	102	3	)	)	PUNCT
njb-38	102	4	the	the	DET
njb-38	102	5	pet30a	pet30a	NUM
njb-38	102	6	bacterial	bacterial	ADJ
njb-38	102	7	extract	extract	NOUN
njb-38	102	8	before	before	ADP
njb-38	102	9	induction	induction	NOUN
njb-38	102	10	(	(	PUNCT
njb-38	102	11	lane	lane	NOUN
njb-38	102	12	1	1	NUM
njb-38	102	13	)	)	PUNCT
njb-38	102	14	,	,	PUNCT
njb-38	102	15	molecular	molecular	ADJ
njb-38	102	16	marker	marker	NOUN
njb-38	102	17	(	(	PUNCT
njb-38	102	18	lane	lane	NOUN
njb-38	102	19	3	3	NUM
njb-38	102	20	)	)	PUNCT
njb-38	102	21	and	and	CCONJ
njb-38	102	22	three	three	NUM
njb-38	102	23	hour	hour	NOUN
njb-38	102	24	after	after	ADP
njb-38	102	25	induction	induction	NOUN
njb-38	102	26	(	(	PUNCT
njb-38	102	27	lane	lane	NOUN
njb-38	102	28	2	2	NUM
njb-38	102	29	,	,	PUNCT
njb-38	102	30	4	4	NUM
njb-38	102	31	,	,	PUNCT
njb-38	102	32	5	5	NUM
njb-38	102	33	and	and	CCONJ
njb-38	102	34	6	6	NUM
njb-38	102	35	)	)	PUNCT
njb-38	102	36	with	with	ADP
njb-38	102	37	50	50	NUM
njb-38	102	38	m	m	PROPN
njb-38	102	39	,	,	PUNCT
njb-38	102	40	100	100	NUM
njb-38	102	41	m	m	PROPN
njb-38	102	42	,	,	PUNCT
njb-38	102	43	200	200	NUM
njb-38	102	44	m	m	NOUN
njb-38	102	45	and	and	CCONJ
njb-38	102	46	500	500	NUM
njb-38	102	47	m	m	PROPN
njb-38	102	48	iptg	iptg	NOUN
njb-38	102	49	respectively	respectively	ADV
njb-38	102	50	.	.	PUNCT
njb-38	103	1	(	(	PUNCT
njb-38	103	2	c	c	X
njb-38	103	3	)	)	PUNCT
njb-38	103	4	purification	purification	NOUN
njb-38	103	5	profile	profile	NOUN
njb-38	103	6	of	of	ADP
njb-38	103	7	soluble	soluble	ADJ
njb-38	103	8	form	form	NOUN
njb-38	103	9	of	of	ADP
njb-38	103	10	hsp70	hsp70	PROPN
njb-38	103	11	protein	protein	NOUN
njb-38	103	12	by	by	ADP
njb-38	103	13	ninta	ninta	PROPN
njb-38	103	14	affinity	affinity	PROPN
njb-38	103	15	bead	bead	NOUN
njb-38	103	16	affinity	affinity	NOUN
njb-38	103	17	chromatography	chromatography	NOUN
njb-38	103	18	resin	resin	NOUN
njb-38	103	19	.	.	PUNCT
njb-38	104	1	lane	lane	NOUN
njb-38	104	2	1	1	NUM
njb-38	104	3	flow	flow	NOUN
njb-38	104	4	through	through	ADV
njb-38	104	5	,	,	PUNCT
njb-38	104	6	lanes	lane	NOUN
njb-38	104	7	2	2	NUM
njb-38	104	8	and	and	CCONJ
njb-38	104	9	3	3	NUM
njb-38	104	10	,	,	PUNCT
njb-38	104	11	washes	wash	VERB
njb-38	104	12	with	with	ADP
njb-38	104	13	20	20	NUM
njb-38	104	14	mm	mm	NOUN
njb-38	104	15	and	and	CCONJ
njb-38	104	16	60	60	NUM
njb-38	104	17	mm	mm	NOUN
njb-38	104	18	immidazole	immidazole	ADJ
njb-38	104	19	,	,	PUNCT
njb-38	104	20	lane	lane	NOUN
njb-38	104	21	4	4	NUM
njb-38	104	22	-	-	SYM
njb-38	104	23	7	7	NUM
njb-38	104	24	elutes	elute	NOUN
njb-38	104	25	using	use	VERB
njb-38	104	26	100	100	NUM
njb-38	104	27	mm	mm	PROPN
njb-38	104	28	,	,	PUNCT
njb-38	104	29	150	150	NUM
njb-38	104	30	mm	mm	PROPN
njb-38	104	31	,	,	PUNCT
njb-38	104	32	200	200	NUM
njb-38	104	33	mm	mm	NOUN
njb-38	104	34	,	,	PUNCT
njb-38	104	35	and	and	CCONJ
njb-38	104	36	250	250	NUM
njb-38	104	37	mm	mm	NOUN
njb-38	104	38	immidazole	immidazole	ADJ
njb-38	104	39	,	,	PUNCT
njb-38	104	40	lane	lane	NOUN
njb-38	104	41	8	8	NUM
njb-38	104	42	nibeads	nibead	NOUN
njb-38	104	43	,	,	PUNCT
njb-38	104	44	lane	lane	NOUN
njb-38	104	45	9blank	9blank	NUM
njb-38	104	46	.	.	PUNCT
njb-38	105	1	all	all	DET
njb-38	105	2	the	the	DET
njb-38	105	3	purification	purification	NOUN
njb-38	105	4	fractions	fraction	NOUN
njb-38	105	5	were	be	AUX
njb-38	105	6	run	run	VERB
njb-38	105	7	along	along	ADP
njb-38	105	8	with	with	ADP
njb-38	105	9	the	the	DET
njb-38	105	10	molecular	molecular	ADJ
njb-38	105	11	weight	weight	NOUN
njb-38	105	12	protein	protein	NOUN
njb-38	105	13	marker	marker	NOUN
njb-38	105	14	(	(	PUNCT
njb-38	105	15	lane	lane	NOUN
njb-38	105	16	m	m	NUM
njb-38	105	17	)	)	PUNCT
njb-38	105	18	.	.	PUNCT
njb-38	106	1	polyclonal	polyclonal	ADJ
njb-38	106	2	antibody	antibody	NOUN
njb-38	106	3	production	production	NOUN
njb-38	106	4	and	and	CCONJ
njb-38	106	5	western	western	ADJ
njb-38	106	6	blotting	blot	VERB
njb-38	106	7	western	western	ADJ
njb-38	106	8	blot	blot	NOUN
njb-38	106	9	using	use	VERB
njb-38	106	10	size	size	NOUN
njb-38	106	11	-	-	PUNCT
njb-38	106	12	fractionated	fractionate	VERB
njb-38	106	13	parasite	parasite	ADJ
njb-38	106	14	protein	protein	NOUN
njb-38	106	15	,	,	PUNCT
njb-38	106	16	antiserum	antiserum	NOUN
njb-38	106	17	could	could	AUX
njb-38	106	18	detect	detect	VERB
njb-38	106	19	a	a	DET
njb-38	106	20	band	band	NOUN
njb-38	106	21	of	of	ADP
njb-38	106	22	anticipated	anticipated	PROPN
njb-38	106	23	l.	l.	PROPN
njb-38	106	24	donovani	donovani	PROPN
njb-38	106	25	hsp70	hsp70	PROPN
njb-38	106	26	size	size	VERB
njb-38	106	27	~70	~70	NUM
njb-38	106	28	kda	kda	NOUN
njb-38	106	29	in	in	ADP
njb-38	106	30	promastigote	promastigote	ADJ
njb-38	106	31	extracts	extract	NOUN
njb-38	106	32	.	.	PUNCT
njb-38	107	1	in	in	ADP
njb-38	107	2	antimony	antimony	NOUN
njb-38	107	3	resistant	resistant	ADJ
njb-38	107	4	l.	l.	ADJ
njb-38	107	5	tarentolae	tarentolae	ADJ
njb-38	107	6	mutants	mutant	NOUN
njb-38	107	7	,	,	PUNCT
njb-38	107	8	hsp70	hsp70	PROPN
njb-38	107	9	was	be	AUX
njb-38	107	10	reported	report	VERB
njb-38	107	11	to	to	PART
njb-38	107	12	increase	increase	VERB
njb-38	107	13	more	more	ADJ
njb-38	107	14	than	than	ADP
njb-38	107	15	4	4	NUM
njb-38	107	16	-	-	ADJ
njb-38	107	17	fold	fold	ADJ
njb-38	107	18	compared	compare	VERB
njb-38	107	19	to	to	ADP
njb-38	107	20	the	the	DET
njb-38	107	21	wild	wild	ADJ
njb-38	107	22	-	-	PUNCT
njb-38	107	23	type	type	NOUN
njb-38	107	24	cells	cell	NOUN
njb-38	107	25	[	[	PUNCT
njb-38	107	26	10	10	NUM
njb-38	107	27	]	]	PUNCT
njb-38	107	28	.	.	PUNCT
njb-38	108	1	the	the	DET
njb-38	108	2	overexpression	overexpression	NOUN
njb-38	108	3	of	of	ADP
njb-38	108	4	hsp70	hsp70	NOUN
njb-38	108	5	remained	remain	VERB
njb-38	108	6	stable	stable	ADJ
njb-38	108	7	in	in	ADP
njb-38	108	8	l.	l.	ADJ
njb-38	108	9	tarentolae	tarentolae	PROPN
njb-38	108	10	mutants	mutant	NOUN
njb-38	108	11	after	after	ADP
njb-38	108	12	several	several	ADJ
njb-38	108	13	hundreds	hundred	NOUN
njb-38	108	14	of	of	ADP
njb-38	108	15	passages	passage	NOUN
njb-38	108	16	without	without	ADP
njb-38	108	17	drug	drug	NOUN
njb-38	108	18	[	[	PUNCT
njb-38	108	19	10	10	NUM
njb-38	108	20	]	]	PUNCT
njb-38	108	21	.	.	PUNCT
njb-38	109	1	to	to	PART
njb-38	109	2	test	test	VERB
njb-38	109	3	the	the	DET
njb-38	109	4	link	link	NOUN
njb-38	109	5	between	between	ADP
njb-38	109	6	hsp70	hsp70	PROPN
njb-38	109	7	expression	expression	NOUN
njb-38	109	8	and	and	CCONJ
njb-38	109	9	resistant	resistant	ADJ
njb-38	109	10	phenotype	phenotype	NOUN
njb-38	109	11	in	in	ADP
njb-38	109	12	antimony	antimony	NOUN
njb-38	109	13	resistant	resistant	ADJ
njb-38	109	14	l.	l.	PROPN
njb-38	109	15	donovani	donovani	PROPN
njb-38	109	16	field	field	NOUN
njb-38	109	17	isolates	isolate	NOUN
njb-38	109	18	,	,	PUNCT
njb-38	109	19	we	we	PRON
njb-38	109	20	performed	perform	VERB
njb-38	109	21	western	western	ADJ
njb-38	109	22	blot	blot	NOUN
njb-38	109	23	analysis	analysis	NOUN
njb-38	109	24	using	use	VERB
njb-38	109	25	polyclonal	polyclonal	ADJ
njb-38	109	26	antibody	antibody	NOUN
njb-38	109	27	.	.	PUNCT
njb-38	110	1	a	a	DET
njb-38	110	2	western	western	ADJ
njb-38	110	3	blot	blot	NOUN
njb-38	110	4	using	use	VERB
njb-38	110	5	equal	equal	ADJ
njb-38	110	6	amount	amount	NOUN
njb-38	110	7	of	of	ADP
njb-38	110	8	parasite	parasite	ADJ
njb-38	110	9	protein	protein	NOUN
njb-38	110	10	(	(	PUNCT
njb-38	110	11	50	50	NUM
njb-38	110	12	µg	µg	NOUN
njb-38	110	13	each	each	PRON
njb-38	110	14	)	)	PUNCT
njb-38	110	15	extract	extract	NOUN
njb-38	110	16	from	from	ADP
njb-38	110	17	antimony	antimony	NOUN
njb-38	110	18	sensitive	sensitive	ADJ
njb-38	110	19	(	(	PUNCT
njb-38	110	20	ag83	ag83	PROPN
njb-38	110	21	-	-	PUNCT
njb-38	110	22	s	s	PROPN
njb-38	110	23	and	and	CCONJ
njb-38	110	24	2001	2001	NUM
njb-38	110	25	-	-	PUNCT
njb-38	110	26	s	s	PROPN
njb-38	110	27	)	)	PUNCT
njb-38	110	28	and	and	CCONJ
njb-38	110	29	resistant	resistant	ADJ
njb-38	110	30	(	(	PUNCT
njb-38	110	31	ge1	ge1	NOUN
njb-38	110	32	-	-	PUNCT
njb-38	110	33	r	r	NOUN
njb-38	110	34	,	,	PUNCT
njb-38	110	35	41	41	NUM
njb-38	110	36	-	-	PUNCT
njb-38	110	37	r	r	NOUN
njb-38	110	38	and	and	CCONJ
njb-38	110	39	ns2	ns2	NOUN
njb-38	110	40	-	-	PUNCT
njb-38	110	41	r	r	SYM
njb-38	110	42	)	)	PUNCT
njb-38	110	43	field	field	NOUN
njb-38	110	44	isolate	isolate	VERB
njb-38	110	45	did	do	AUX
njb-38	110	46	not	not	PART
njb-38	110	47	show	show	VERB
njb-38	110	48	any	any	DET
njb-38	110	49	differential	differential	ADJ
njb-38	110	50	expression	expression	NOUN
njb-38	110	51	of	of	ADP
njb-38	110	52	hsp70	hsp70	PROPN
njb-38	110	53	protein	protein	NOUN
njb-38	110	54	(	(	PUNCT
njb-38	110	55	figure	figure	NOUN
njb-38	110	56	4	4	NUM
njb-38	110	57	)	)	PUNCT
njb-38	110	58	.	.	PUNCT
njb-38	111	1	figure	figure	VERB
njb-38	111	2	4	4	NUM
njb-38	111	3	.	.	PUNCT
njb-38	112	1	western	western	ADJ
njb-38	112	2	blotting	blotting	NOUN
njb-38	112	3	using	use	VERB
njb-38	112	4	anti	anti	ADJ
njb-38	112	5	-	-	ADJ
njb-38	112	6	his	his	PRON
njb-38	112	7	-	-	PUNCT
njb-38	112	8	hsp70	hsp70	NOUN
njb-38	112	9	antibody	antibody	NOUN
njb-38	112	10	raised	raise	VERB
njb-38	112	11	against	against	ADP
njb-38	112	12	ldhsp70	ldhsp70	NOUN
njb-38	112	13	in	in	ADP
njb-38	112	14	balb	balb	PROPN
njb-38	112	15	/	/	SYM
njb-38	112	16	c	c	NOUN
njb-38	112	17	mice	mouse	NOUN
njb-38	112	18	.	.	PUNCT
njb-38	113	1	hsp70	hsp70	PROPN
njb-38	113	2	protein	protein	NOUN
njb-38	113	3	in	in	ADP
njb-38	113	4	fractionated	fractionated	ADJ
njb-38	113	5	extracts	extract	NOUN
njb-38	113	6	obtained	obtain	VERB
njb-38	113	7	from	from	ADP
njb-38	113	8	sag	sag	PROPN
njb-38	113	9	-	-	PUNCT
njb-38	113	10	s	s	NOUN
njb-38	113	11	and	and	CCONJ
njb-38	113	12	sag	sag	ADJ
njb-38	113	13	-	-	PUNCT
njb-38	113	14	r	r	NOUN
njb-38	113	15	cultures	culture	NOUN
njb-38	113	16	harvested	harvest	VERB
njb-38	113	17	after	after	ADP
njb-38	113	18	48	48	NUM
njb-38	113	19	h	h	NOUN
njb-38	113	20	of	of	ADP
njb-38	113	21	growth	growth	NOUN
njb-38	113	22	.	.	PUNCT
njb-38	114	1	the	the	DET
njb-38	114	2	same	same	ADJ
njb-38	114	3	blots	blot	NOUN
njb-38	114	4	were	be	AUX
njb-38	114	5	reprobed	reprobe	VERB
njb-38	114	6	with	with	ADP
njb-38	114	7	antibody	antibody	NOUN
njb-38	114	8	against	against	ADP
njb-38	114	9	βtubulin	βtubulin	PROPN
njb-38	114	10	protein	protein	NOUN
njb-38	114	11	to	to	PART
njb-38	114	12	normalize	normalize	VERB
njb-38	114	13	the	the	DET
njb-38	114	14	loading	loading	NOUN
njb-38	114	15	on	on	ADP
njb-38	114	16	to	to	ADP
njb-38	114	17	each	each	DET
njb-38	114	18	lane	lane	NOUN
njb-38	114	19	of	of	ADP
njb-38	114	20	the	the	DET
njb-38	114	21	gel	gel	NOUN
njb-38	114	22	.	.	PUNCT
njb-38	115	1	southern	southern	ADJ
njb-38	115	2	blot	blot	PROPN
njb-38	115	3	hybridization	hybridization	NOUN
njb-38	115	4	of	of	ADP
njb-38	115	5	hsp70	hsp70	PROPN
njb-38	115	6	gene	gene	NOUN
njb-38	115	7	the	the	DET
njb-38	115	8	copy	copy	NOUN
njb-38	115	9	number	number	NOUN
njb-38	115	10	of	of	ADP
njb-38	115	11	hsp70	hsp70	NOUN
njb-38	115	12	gene	gene	NOUN
njb-38	115	13	varies	vary	VERB
njb-38	115	14	considerably	considerably	ADV
njb-38	115	15	among	among	ADP
njb-38	115	16	various	various	ADJ
njb-38	115	17	leishmania	leishmania	NOUN
njb-38	115	18	species	specie	NOUN
njb-38	115	19	[	[	PUNCT
njb-38	115	20	6	6	NUM
njb-38	115	21	,	,	PUNCT
njb-38	115	22	10	10	NUM
njb-38	115	23	,	,	PUNCT
njb-38	115	24	14	14	NUM
njb-38	115	25	]	]	PUNCT
njb-38	115	26	.	.	PUNCT
njb-38	116	1	to	to	PART
njb-38	116	2	determine	determine	VERB
njb-38	116	3	hsp70	hsp70	NOUN
njb-38	116	4	gene	gene	NOUN
njb-38	116	5	copy	copy	NOUN
njb-38	116	6	number	number	NOUN
njb-38	116	7	in	in	ADP
njb-38	116	8	l.	l.	PROPN
njb-38	116	9	donovani	donovani	PROPN
njb-38	116	10	field	field	PROPN
njb-38	116	11	isolates	isolate	NOUN
njb-38	116	12	,	,	PUNCT
njb-38	116	13	southern	southern	ADJ
njb-38	116	14	blot	blot	NOUN
njb-38	116	15	analysis	analysis	NOUN
njb-38	116	16	was	be	AUX
njb-38	116	17	performed	perform	VERB
njb-38	116	18	using	use	VERB
njb-38	116	19	1.9	1.9	NUM
njb-38	116	20	kb	kb	NOUN
njb-38	116	21	hsp70	hsp70	PROPN
njb-38	116	22	pcr	pcr	NOUN
njb-38	116	23	product	product	NOUN
njb-38	116	24	as	as	ADP
njb-38	116	25	a	a	DET
njb-38	116	26	probe	probe	NOUN
njb-38	116	27	as	as	SCONJ
njb-38	116	28	described	describe	VERB
njb-38	116	29	under	under	ADP
njb-38	116	30	general	general	ADJ
njb-38	116	31	materials	material	NOUN
njb-38	116	32	and	and	CCONJ
njb-38	116	33	methods	method	NOUN
njb-38	116	34	.	.	PUNCT
njb-38	117	1	hindiii	hindiii	NOUN
njb-38	117	2	which	which	PRON
njb-38	117	3	digests	digest	VERB
njb-38	117	4	outside	outside	ADP
njb-38	117	5	the	the	DET
njb-38	117	6	gene	gene	NOUN
njb-38	117	7	producing	produce	VERB
njb-38	117	8	a	a	DET
njb-38	117	9	10	10	NUM
njb-38	117	10	kb	kb	PROPN
njb-38	117	11	restriction	restriction	NOUN
njb-38	117	12	fragment	fragment	NOUN
njb-38	117	13	(	(	PUNCT
njb-38	117	14	expected	expect	VERB
njb-38	117	15	size	size	NOUN
njb-38	117	16	)	)	PUNCT
njb-38	117	17	was	be	AUX
njb-38	117	18	used	use	VERB
njb-38	117	19	to	to	PART
njb-38	117	20	check	check	VERB
njb-38	117	21	the	the	DET
njb-38	117	22	copy	copy	NOUN
njb-38	117	23	number	number	NOUN
njb-38	117	24	of	of	ADP
njb-38	117	25	the	the	DET
njb-38	117	26	gene	gene	NOUN
njb-38	117	27	.	.	PUNCT
njb-38	118	1	the	the	DET
njb-38	118	2	probe	probe	NOUN
njb-38	118	3	hybridized	hybridize	VERB
njb-38	118	4	to	to	ADP
njb-38	118	5	a	a	DET
njb-38	118	6	single	single	ADJ
njb-38	118	7	fragment	fragment	NOUN
njb-38	118	8	of	of	ADP
njb-38	118	9	10	10	NUM
njb-38	118	10	kb	kb	NOUN
njb-38	118	11	in	in	ADP
njb-38	118	12	all	all	DET
njb-38	118	13	the	the	DET
njb-38	118	14	field	field	NOUN
njb-38	118	15	isolates	isolate	VERB
njb-38	118	16	(	(	PUNCT
njb-38	118	17	figure	figure	NOUN
njb-38	118	18	5	5	NUM
njb-38	118	19	)	)	PUNCT
njb-38	118	20	indicating	indicate	VERB
njb-38	118	21	that	that	SCONJ
njb-38	118	22	hsp70	hsp70	PROPN
njb-38	118	23	exists	exist	VERB
njb-38	118	24	as	as	ADP
njb-38	118	25	a	a	DET
njb-38	118	26	single	single	ADJ
njb-38	118	27	copy	copy	NOUN
njb-38	118	28	gene	gene	NOUN
njb-38	118	29	in	in	ADP
njb-38	118	30	these	these	DET
njb-38	118	31	strains	strain	NOUN
njb-38	118	32	.	.	PUNCT
njb-38	119	1	further	far	ADV
njb-38	119	2	,	,	PUNCT
njb-38	119	3	we	we	PRON
njb-38	119	4	checked	check	VERB
njb-38	119	5	the	the	DET
njb-38	119	6	chromosomal	chromosomal	ADJ
njb-38	119	7	localization	localization	NOUN
njb-38	119	8	of	of	ADP
njb-38	119	9	hsp70	hsp70	PROPN
njb-38	119	10	gene	gene	NOUN
njb-38	119	11	by	by	ADP
njb-38	119	12	southern	southern	ADJ
njb-38	119	13	blot	blot	NOUN
njb-38	119	14	hybridization	hybridization	NOUN
njb-38	119	15	of	of	ADP
njb-38	119	16	pfge	pfge	NOUN
njb-38	119	17	blot	blot	NOUN
njb-38	119	18	.	.	PUNCT
njb-38	120	1	the	the	DET
njb-38	120	2	hsp70	hsp70	PROPN
njb-38	120	3	probe	probe	NOUN
njb-38	120	4	hybridized	hybridize	VERB
njb-38	120	5	to	to	ADP
njb-38	120	6	a	a	DET
njb-38	120	7	single	single	ADJ
njb-38	120	8	chromosomal	chromosomal	ADJ
njb-38	120	9	band	band	NOUN
njb-38	120	10	of	of	ADP
njb-38	120	11	1.2	1.2	NUM
njb-38	120	12	mb	mb	ADP
njb-38	120	13	region	region	NOUN
njb-38	120	14	that	that	PRON
njb-38	120	15	corresponds	correspond	VERB
njb-38	120	16	to	to	ADP
njb-38	120	17	the	the	DET
njb-38	120	18	chromosome	chromosome	NOUN
njb-38	120	19	number	number	NOUN
njb-38	120	20	28	28	NUM
njb-38	120	21	of	of	ADP
njb-38	120	22	the	the	DET
njb-38	120	23	l.	l.	PROPN
njb-38	120	24	infantum	infantum	PROPN
njb-38	120	25	genome	genome	NOUN
njb-38	120	26	database	database	NOUN
njb-38	120	27	(	(	PUNCT
njb-38	120	28	figure	figure	NOUN
njb-38	120	29	6	6	NUM
njb-38	120	30	)	)	PUNCT
njb-38	120	31	.	.	PUNCT
njb-38	121	1	nepal	nepal	ADJ
njb-38	121	2	journal	journal	PROPN
njb-38	121	3	of	of	ADP
njb-38	121	4	biotechnology	biotechnology	NOUN
njb-38	121	5	.	.	PUNCT
njb-38	122	1	dec	dec	PROPN
njb-38	122	2	.	.	PROPN
njb-38	122	3	2015	2015	NUM
njb-38	122	4	vol	vol	NOUN
njb-38	122	5	.	.	PROPN
njb-38	123	1	3	3	NUM
njb-38	123	2	,	,	PUNCT
njb-38	123	3	no	no	INTJ
njb-38	123	4	.	.	PUNCT
njb-38	124	1	1:22	1:22	NUM
njb-38	124	2	-	-	SYM
njb-38	124	3	28	28	NUM
njb-38	124	4	maharjan	maharjan	NOUN
njb-38	124	5	and	and	CCONJ
njb-38	124	6	madhubala	madhubala	PROPN
njb-38	124	7	©	©	PROPN
njb-38	124	8	njb	njb	PROPN
njb-38	124	9	,	,	PUNCT
njb-38	124	10	biotechnology	biotechnology	NOUN
njb-38	124	11	society	society	NOUN
njb-38	124	12	of	of	ADP
njb-38	124	13	nepal	nepal	ADJ
njb-38	124	14	26	26	NUM
njb-38	124	15	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-38	124	16	figure	figure	NOUN
njb-38	124	17	5	5	NUM
njb-38	124	18	.	.	PUNCT
njb-38	125	1	southern	southern	ADJ
njb-38	125	2	blot	blot	NOUN
njb-38	125	3	analysis	analysis	NOUN
njb-38	125	4	of	of	ADP
njb-38	125	5	hsp70	hsp70	PROPN
njb-38	125	6	gene	gene	NOUN
njb-38	125	7	in	in	ADP
njb-38	125	8	sag	sag	PROPN
njb-38	125	9	–	–	PUNCT
njb-38	125	10	s	s	PROPN
njb-38	125	11	and	and	CCONJ
njb-38	125	12	sag	sag	ADJ
njb-38	125	13	–	–	PUNCT
njb-38	125	14	r	r	PROPN
njb-38	125	15	leishmania	leishmania	PROPN
njb-38	125	16	donovani	donovani	PROPN
njb-38	125	17	field	field	NOUN
njb-38	125	18	isolates	isolate	NOUN
njb-38	125	19	.	.	PUNCT
njb-38	126	1	total	total	ADJ
njb-38	126	2	genomic	genomic	ADJ
njb-38	126	3	dna	dna	NOUN
njb-38	126	4	of	of	ADP
njb-38	126	5	leishmania	leishmania	NOUN
njb-38	126	6	strains	strain	NOUN
njb-38	126	7	were	be	AUX
njb-38	126	8	isolated	isolate	VERB
njb-38	126	9	and	and	CCONJ
njb-38	126	10	digested	digest	VERB
njb-38	126	11	with	with	ADP
njb-38	126	12	hindiii	hindiii	ADJ
njb-38	126	13	restriction	restriction	NOUN
njb-38	126	14	enzyme	enzyme	NOUN
njb-38	126	15	.	.	PUNCT
njb-38	127	1	the	the	DET
njb-38	127	2	blot	blot	NOUN
njb-38	127	3	was	be	AUX
njb-38	127	4	probed	probe	VERB
njb-38	127	5	with	with	ADP
njb-38	127	6	1.9	1.9	NUM
njb-38	127	7	kb	kb	PROPN
njb-38	127	8	full	full	ADJ
njb-38	127	9	length	length	NOUN
njb-38	127	10	hsp70	hsp70	PROPN
njb-38	127	11	gene	gene	NOUN
njb-38	127	12	.	.	PUNCT
njb-38	128	1	the	the	DET
njb-38	128	2	blot	blot	NOUN
njb-38	128	3	was	be	AUX
njb-38	128	4	reprobed	reprobe	VERB
njb-38	128	5	with	with	ADP
njb-38	128	6	α	α	PRON
njb-38	128	7	-	-	PUNCT
njb-38	128	8	tubulin	tubulin	NOUN
njb-38	128	9	to	to	PART
njb-38	128	10	monitor	monitor	VERB
njb-38	128	11	the	the	DET
njb-38	128	12	amount	amount	NOUN
njb-38	128	13	of	of	ADP
njb-38	128	14	digested	digested	ADJ
njb-38	128	15	dna	dna	NOUN
njb-38	128	16	layered	layer	VERB
njb-38	128	17	on	on	ADP
njb-38	128	18	the	the	DET
njb-38	128	19	gel	gel	NOUN
njb-38	128	20	.	.	PUNCT
njb-38	129	1	figure	figure	VERB
njb-38	129	2	6	6	NUM
njb-38	129	3	.	.	PUNCT
njb-38	129	4	pfge	pfge	NOUN
njb-38	129	5	analysis	analysis	NOUN
njb-38	129	6	of	of	ADP
njb-38	129	7	sag	sag	PROPN
njb-38	129	8	-	-	PUNCT
njb-38	129	9	s	s	NOUN
njb-38	129	10	and	and	CCONJ
njb-38	129	11	sag	sag	ADJ
njb-38	129	12	-	-	PUNCT
njb-38	129	13	r	r	NOUN
njb-38	129	14	l.	l.	PROPN
njb-38	129	15	donovani	donovani	PROPN
njb-38	129	16	isolates	isolate	VERB
njb-38	129	17	indicating	indicate	VERB
njb-38	129	18	chromosomal	chromosomal	ADJ
njb-38	129	19	localization	localization	NOUN
njb-38	129	20	of	of	ADP
njb-38	129	21	hsp70	hsp70	PROPN
njb-38	129	22	gene	gene	NOUN
njb-38	129	23	.	.	PUNCT
njb-38	130	1	chromosomes	chromosome	NOUN
njb-38	130	2	of	of	ADP
njb-38	130	3	l.	l.	PROPN
njb-38	130	4	donovani	donovani	PROPN
njb-38	130	5	isolates	isolate	NOUN
njb-38	130	6	were	be	AUX
njb-38	130	7	separated	separate	VERB
njb-38	130	8	by	by	ADP
njb-38	130	9	chef	chef	NOUN
njb-38	130	10	and	and	CCONJ
njb-38	130	11	southern	southern	ADJ
njb-38	130	12	blots	blot	NOUN
njb-38	130	13	were	be	AUX
njb-38	130	14	hybridized	hybridize	VERB
njb-38	130	15	with	with	ADP
njb-38	130	16	the	the	DET
njb-38	130	17	hsp70	hsp70	PROPN
njb-38	130	18	probe	probe	NOUN
njb-38	130	19	.	.	PUNCT
njb-38	131	1	the	the	DET
njb-38	131	2	size	size	NOUN
njb-38	131	3	of	of	ADP
njb-38	131	4	hybridizing	hybridize	VERB
njb-38	131	5	band	band	NOUN
njb-38	131	6	was	be	AUX
njb-38	131	7	identified	identify	VERB
njb-38	131	8	using	use	VERB
njb-38	131	9	chromosomes	chromosome	NOUN
njb-38	131	10	of	of	ADP
njb-38	131	11	s.	s.	PROPN
njb-38	131	12	cerevisae	cerevisae	PROPN
njb-38	131	13	as	as	ADP
njb-38	131	14	marker	marker	NOUN
njb-38	131	15	figure	figure	NOUN
njb-38	131	16	7	7	NUM
njb-38	131	17	.	.	PUNCT
njb-38	132	1	expression	expression	VERB
njb-38	132	2	analysis	analysis	NOUN
njb-38	132	3	of	of	ADP
njb-38	132	4	hsp70	hsp70	PROPN
njb-38	132	5	gene	gene	NOUN
njb-38	132	6	in	in	ADP
njb-38	132	7	l.	l.	PROPN
njb-38	132	8	donovani	donovani	PROPN
njb-38	132	9	northern	northern	ADJ
njb-38	132	10	blot	blot	NOUN
njb-38	132	11	analysis	analysis	NOUN
njb-38	132	12	of	of	ADP
njb-38	132	13	mrna	mrna	NOUN
njb-38	132	14	from	from	ADP
njb-38	132	15	sag	sag	PROPN
njb-38	132	16	-	-	PUNCT
njb-38	132	17	s	s	NOUN
njb-38	132	18	and	and	CCONJ
njb-38	132	19	sag	sag	ADJ
njb-38	132	20	-	-	PUNCT
njb-38	132	21	r	r	NOUN
njb-38	132	22	isolates	isolate	NOUN
njb-38	132	23	(	(	PUNCT
njb-38	132	24	log	log	NOUN
njb-38	132	25	phase	phase	NOUN
njb-38	132	26	culture	culture	NOUN
njb-38	132	27	)	)	PUNCT
njb-38	132	28	.	.	PUNCT
njb-38	133	1	15	15	NUM
njb-38	133	2	µg	µg	NOUN
njb-38	133	3	of	of	ADP
njb-38	133	4	total	total	ADJ
njb-38	133	5	rna	rna	PROPN
njb-38	133	6	was	be	AUX
njb-38	133	7	loaded	load	VERB
njb-38	133	8	per	per	ADP
njb-38	133	9	lane	lane	NOUN
njb-38	133	10	,	,	PUNCT
njb-38	133	11	transferred	transfer	VERB
njb-38	133	12	and	and	CCONJ
njb-38	133	13	hybridized	hybridize	VERB
njb-38	133	14	with	with	ADP
njb-38	133	15	hsp70	hsp70	PROPN
njb-38	133	16	probe	probe	NOUN
njb-38	133	17	.	.	PUNCT
njb-38	134	1	a	a	DET
njb-38	134	2	α	α	ADJ
njb-38	134	3	-	-	PUNCT
njb-38	134	4	tubulin	tubulin	NOUN
njb-38	134	5	probe	probe	NOUN
njb-38	134	6	and	and	CCONJ
njb-38	134	7	was	be	AUX
njb-38	134	8	used	use	VERB
njb-38	134	9	to	to	PART
njb-38	134	10	monitor	monitor	VERB
njb-38	134	11	the	the	DET
njb-38	134	12	amount	amount	NOUN
njb-38	134	13	of	of	ADP
njb-38	134	14	rna	rna	PROPN
njb-38	134	15	layerd	layerd	NOUN
njb-38	134	16	on	on	ADP
njb-38	134	17	the	the	DET
njb-38	134	18	gel	gel	NOUN
njb-38	134	19	.	.	PUNCT
njb-38	135	1	the	the	DET
njb-38	135	2	rrna	rrna	PROPN
njb-38	135	3	stained	stain	VERB
njb-38	135	4	with	with	ADP
njb-38	135	5	ethidium	ethidium	NOUN
njb-38	135	6	bromide	bromide	NOUN
njb-38	135	7	was	be	AUX
njb-38	135	8	used	use	VERB
njb-38	135	9	to	to	PART
njb-38	135	10	normalize	normalize	VERB
njb-38	135	11	the	the	DET
njb-38	135	12	rna	rna	PROPN
njb-38	135	13	loading	loading	NOUN
njb-38	135	14	.	.	PUNCT
njb-38	136	1	northern	northern	ADJ
njb-38	136	2	blot	blot	NOUN
njb-38	136	3	hybridization	hybridization	NOUN
njb-38	136	4	of	of	ADP
njb-38	136	5	hsp70	hsp70	PROPN
njb-38	136	6	gene	gene	NOUN
njb-38	136	7	since	since	SCONJ
njb-38	136	8	,	,	PUNCT
njb-38	136	9	we	we	PRON
njb-38	136	10	could	could	AUX
njb-38	136	11	n’t	not	PART
njb-38	136	12	find	find	VERB
njb-38	136	13	any	any	DET
njb-38	136	14	difference	difference	NOUN
njb-38	136	15	in	in	ADP
njb-38	136	16	hsp70	hsp70	PROPN
njb-38	136	17	protein	protein	NOUN
njb-38	136	18	expression	expression	NOUN
njb-38	136	19	and	and	CCONJ
njb-38	136	20	copy	copy	NOUN
njb-38	136	21	number	number	NOUN
njb-38	136	22	of	of	ADP
njb-38	136	23	gene	gene	NOUN
njb-38	136	24	in	in	ADP
njb-38	136	25	between	between	ADP
njb-38	136	26	sags	sag	NOUN
njb-38	136	27	and	and	CCONJ
njb-38	136	28	sag	sag	ADJ
njb-38	136	29	-	-	PUNCT
njb-38	136	30	r	r	NOUN
njb-38	136	31	l.	l.	PROPN
njb-38	136	32	donovani	donovani	PROPN
njb-38	136	33	field	field	PROPN
njb-38	136	34	isolates	isolate	NOUN
njb-38	136	35	,	,	PUNCT
njb-38	136	36	we	we	PRON
njb-38	136	37	checked	check	VERB
njb-38	136	38	transcript	transcript	NOUN
njb-38	136	39	level	level	NOUN
njb-38	136	40	of	of	ADP
njb-38	136	41	hsp70	hsp70	NOUN
njb-38	136	42	gene	gene	NOUN
njb-38	136	43	expression	expression	NOUN
njb-38	136	44	.	.	PUNCT
njb-38	137	1	northern	northern	ADJ
njb-38	137	2	blotting	blotting	NOUN
njb-38	137	3	of	of	ADP
njb-38	137	4	sag	sag	PROPN
njb-38	137	5	-	-	PUNCT
njb-38	137	6	s	s	NOUN
njb-38	137	7	and	and	CCONJ
njb-38	137	8	sag	sag	ADJ
njb-38	137	9	-	-	PUNCT
njb-38	137	10	r	r	NOUN
njb-38	137	11	l.	l.	PROPN
njb-38	137	12	donovani	donovani	PROPN
njb-38	137	13	field	field	PROPN
njb-38	137	14	isolates	isolate	NOUN
njb-38	137	15	revealed	reveal	VERB
njb-38	137	16	a	a	DET
njb-38	137	17	single	single	ADJ
njb-38	137	18	transcript	transcript	NOUN
njb-38	137	19	of	of	ADP
njb-38	137	20	~	~	PUNCT
njb-38	137	21	3.5	3.5	NUM
njb-38	137	22	kb	kb	NOUN
njb-38	137	23	in	in	ADP
njb-38	137	24	all	all	DET
njb-38	137	25	the	the	DET
njb-38	137	26	strains	strain	NOUN
njb-38	137	27	(	(	PUNCT
njb-38	137	28	figure	figure	NOUN
njb-38	137	29	7	7	NUM
njb-38	137	30	)	)	PUNCT
njb-38	137	31	.	.	PUNCT
njb-38	138	1	densitometric	densitometric	ADJ
njb-38	138	2	analysis	analysis	NOUN
njb-38	138	3	of	of	ADP
njb-38	138	4	hybridizing	hybridize	VERB
njb-38	138	5	bands	band	NOUN
njb-38	138	6	corresponds	correspond	VERB
njb-38	138	7	to	to	ADP
njb-38	138	8	the	the	DET
njb-38	138	9	internal	internal	ADJ
njb-38	138	10	control	control	NOUN
njb-38	138	11	α	α	PROPN
njb-38	138	12	-	-	PUNCT
njb-38	138	13	tubulin	tubulin	NOUN
njb-38	138	14	showed	show	VERB
njb-38	138	15	similar	similar	ADJ
njb-38	138	16	expression	expression	NOUN
njb-38	138	17	of	of	ADP
njb-38	138	18	hsp70	hsp70	PROPN
njb-38	138	19	gene	gene	NOUN
njb-38	138	20	in	in	ADP
njb-38	138	21	between	between	ADP
njb-38	138	22	sag	sag	NOUN
njb-38	138	23	sensitive	sensitive	ADJ
njb-38	138	24	and	and	CCONJ
njb-38	138	25	resistant	resistant	ADJ
njb-38	138	26	field	field	NOUN
njb-38	138	27	isolates	isolate	NOUN
njb-38	138	28	.	.	PUNCT
njb-38	139	1	induction	induction	NOUN
njb-38	139	2	of	of	ADP
njb-38	139	3	hsp70	hsp70	NOUN
njb-38	139	4	by	by	ADP
njb-38	139	5	sbiii	sbiii	NOUN
njb-38	139	6	in	in	ADP
njb-38	139	7	promastigote	promastigote	PROPN
njb-38	139	8	of	of	ADP
njb-38	139	9	l.	l.	PROPN
njb-38	139	10	donovani	donovani	PROPN
njb-38	139	11	field	field	PROPN
njb-38	139	12	isolate	isolate	VERB
njb-38	139	13	leishmania	leishmania	PROPN
njb-38	139	14	cells	cell	NOUN
njb-38	139	15	respond	respond	VERB
njb-38	139	16	to	to	ADP
njb-38	139	17	sbiii	sbiii	NOUN
njb-38	139	18	exposure	exposure	NOUN
njb-38	139	19	by	by	ADP
njb-38	139	20	increasing	increase	VERB
njb-38	139	21	hsp70	hsp70	NOUN
njb-38	139	22	protein	protein	NOUN
njb-38	140	1	[	[	X
njb-38	140	2	10	10	NUM
njb-38	140	3	]	]	PUNCT
njb-38	140	4	.	.	PUNCT
njb-38	141	1	induction	induction	NOUN
njb-38	141	2	of	of	ADP
njb-38	141	3	hsp70	hsp70	PROPN
njb-38	141	4	was	be	AUX
njb-38	141	5	found	find	VERB
njb-38	141	6	more	more	ADJ
njb-38	141	7	than	than	ADP
njb-38	141	8	four	four	NUM
njb-38	141	9	(	(	PUNCT
njb-38	141	10	>	>	SYM
njb-38	141	11	4	4	NUM
njb-38	141	12	)	)	PUNCT
njb-38	141	13	fold	fold	VERB
njb-38	141	14	in	in	ADP
njb-38	141	15	l.	l.	PROPN
njb-38	141	16	tarentolae	tarentolae	PROPN
njb-38	141	17	and	and	CCONJ
njb-38	141	18	l.	l.	PROPN
njb-38	141	19	infantum	infantum	PROPN
njb-38	141	20	cells	cell	NOUN
njb-38	141	21	when	when	SCONJ
njb-38	141	22	incubated	incubate	VERB
njb-38	141	23	for	for	ADP
njb-38	141	24	24	24	NUM
njb-38	141	25	hours	hour	NOUN
njb-38	141	26	at	at	ADP
njb-38	141	27	ic50	ic50	PROPN
njb-38	141	28	concentration	concentration	NOUN
njb-38	141	29	of	of	ADP
njb-38	141	30	the	the	DET
njb-38	141	31	drug	drug	NOUN
njb-38	141	32	[	[	PUNCT
njb-38	141	33	10	10	NUM
njb-38	141	34	]	]	PUNCT
njb-38	141	35	.	.	PUNCT
njb-38	142	1	to	to	PART
njb-38	142	2	test	test	VERB
njb-38	142	3	whether	whether	SCONJ
njb-38	142	4	strain	strain	NOUN
njb-38	142	5	were	be	AUX
njb-38	142	6	incubated	incubate	VERB
njb-38	142	7	with	with	ADP
njb-38	142	8	15	15	NUM
njb-38	142	9	µm	µm	NOUN
njb-38	142	10	(	(	PUNCT
njb-38	142	11	ic50	ic50	PROPN
njb-38	142	12	concentration	concentration	NOUN
njb-38	142	13	of	of	ADP
njb-38	142	14	ag83	ag83	PROPN
njb-38	142	15	-	-	PUNCT
njb-38	142	16	s	s	PART
njb-38	142	17	)	)	PUNCT
njb-38	142	18	sbiii	sbiii	NOUN
njb-38	142	19	for	for	ADP
njb-38	142	20	24	24	NUM
njb-38	142	21	hours	hour	NOUN
njb-38	142	22	.	.	PUNCT
njb-38	143	1	a	a	DET
njb-38	143	2	time	time	NOUN
njb-38	143	3	dependent	dependent	ADJ
njb-38	143	4	increase	increase	NOUN
njb-38	143	5	of	of	ADP
njb-38	143	6	hsp70	hsp70	PROPN
njb-38	143	7	protein	protein	NOUN
njb-38	143	8	expression	expression	NOUN
njb-38	143	9	in	in	ADP
njb-38	143	10	l.	l.	PROPN
njb-38	143	11	donovani	donovani	PROPN
njb-38	143	12	field	field	NOUN
njb-38	143	13	isolates	isolate	NOUN
njb-38	143	14	was	be	AUX
njb-38	143	15	observed	observe	VERB
njb-38	143	16	.	.	PUNCT
njb-38	144	1	protein	protein	NOUN
njb-38	144	2	expression	expression	NOUN
njb-38	144	3	increased	increase	VERB
njb-38	144	4	by	by	ADP
njb-38	144	5	more	more	ADJ
njb-38	144	6	than	than	ADP
njb-38	144	7	>	>	X
njb-38	144	8	1.7	1.7	NUM
njb-38	144	9	fold	fold	NOUN
njb-38	144	10	at	at	ADP
njb-38	144	11	12	12	NUM
njb-38	144	12	hours	hour	NOUN
njb-38	144	13	and	and	CCONJ
njb-38	144	14	more	more	ADJ
njb-38	144	15	than	than	ADP
njb-38	144	16	three	three	NUM
njb-38	144	17	(	(	PUNCT
njb-38	144	18	>	>	SYM
njb-38	144	19	3	3	NUM
njb-38	144	20	)	)	PUNCT
njb-38	144	21	fold	fold	VERB
njb-38	144	22	after	after	ADP
njb-38	144	23	24	24	NUM
njb-38	144	24	hours	hour	NOUN
njb-38	144	25	compared	compare	VERB
njb-38	144	26	to	to	ADP
njb-38	144	27	the	the	DET
njb-38	144	28	cells	cell	NOUN
njb-38	144	29	incubated	incubate	VERB
njb-38	144	30	without	without	ADP
njb-38	144	31	drug	drug	NOUN
njb-38	144	32	(	(	PUNCT
njb-38	144	33	figure	figure	NOUN
njb-38	144	34	8a	8a	NUM
njb-38	144	35	and	and	CCONJ
njb-38	144	36	8b	8b	NUM
njb-38	144	37	)	)	PUNCT
njb-38	144	38	.	.	PUNCT
njb-38	145	1	trivalent	trivalent	ADJ
njb-38	145	2	antimony	antimony	NOUN
njb-38	145	3	could	could	AUX
njb-38	145	4	induce	induce	VERB
njb-38	145	5	the	the	DET
njb-38	145	6	expression	expression	NOUN
njb-38	145	7	of	of	ADP
njb-38	145	8	hsp70	hsp70	PROPN
njb-38	145	9	protein	protein	NOUN
njb-38	145	10	in	in	ADP
njb-38	145	11	l.	l.	PROPN
njb-38	145	12	donovani	donovani	PROPN
njb-38	145	13	field	field	PROPN
njb-38	145	14	isolates	isolate	NOUN
njb-38	145	15	,	,	PUNCT
njb-38	145	16	we	we	PRON
njb-38	145	17	used	use	VERB
njb-38	145	18	polyclonal	polyclonal	ADJ
njb-38	145	19	antibody	antibody	NOUN
njb-38	145	20	raised	raise	VERB
njb-38	145	21	against	against	ADP
njb-38	145	22	hsp70	hsp70	PROPN
njb-38	145	23	antigen	antigen	PROPN
njb-38	145	24	in	in	ADP
njb-38	145	25	balb	balb	PROPN
njb-38	145	26	/	/	SYM
njb-38	145	27	c	c	NOUN
njb-38	145	28	mice	mouse	NOUN
njb-38	145	29	.	.	PUNCT
njb-38	146	1	promastigotes	promastigote	NOUN
njb-38	146	2	of	of	ADP
njb-38	146	3	l.	l.	PROPN
njb-38	146	4	donovani	donovani	PROPN
njb-38	146	5	(	(	PUNCT
njb-38	146	6	ag83	ag83	PROPN
njb-38	146	7	-	-	PUNCT
njb-38	146	8	s	s	PART
njb-38	146	9	)	)	PUNCT
njb-38	146	10	discussion	discussion	NOUN
njb-38	146	11	physiological	physiological	ADJ
njb-38	146	12	role	role	NOUN
njb-38	146	13	of	of	ADP
njb-38	146	14	the	the	DET
njb-38	146	15	hsp70	hsp70	NOUN
njb-38	146	16	as	as	ADP
njb-38	146	17	a	a	DET
njb-38	146	18	molecular	molecular	ADJ
njb-38	146	19	chaperon	chaperon	NOUN
njb-38	146	20	has	have	AUX
njb-38	146	21	been	be	AUX
njb-38	146	22	well	well	ADV
njb-38	146	23	described	describe	VERB
njb-38	146	24	[	[	X
njb-38	146	25	15	15	NUM
njb-38	146	26	]	]	PUNCT
njb-38	146	27	.	.	PUNCT
njb-38	147	1	induction	induction	NOUN
njb-38	147	2	of	of	ADP
njb-38	147	3	hsp70	hsp70	NOUN
njb-38	147	4	can	can	AUX
njb-38	147	5	protect	protect	VERB
njb-38	147	6	cells	cell	NOUN
njb-38	147	7	by	by	ADP
njb-38	147	8	binding	bind	VERB
njb-38	147	9	to	to	ADP
njb-38	147	10	misfolded	misfolde	VERB
njb-38	147	11	proteins	protein	NOUN
njb-38	147	12	in	in	ADP
njb-38	147	13	variety	variety	NOUN
njb-38	147	14	of	of	ADP
njb-38	147	15	adverse	adverse	ADJ
njb-38	147	16	conditions	condition	NOUN
njb-38	147	17	[	[	PUNCT
njb-38	147	18	16	16	NUM
njb-38	147	19	]	]	PUNCT
njb-38	147	20	.	.	PUNCT
njb-38	148	1	besides	besides	SCONJ
njb-38	148	2	its	its	PRON
njb-38	148	3	physiological	physiological	ADJ
njb-38	148	4	cytoprotective	cytoprotective	ADJ
njb-38	148	5	role	role	NOUN
njb-38	148	6	an	an	DET
njb-38	148	7	increased	increase	VERB
njb-38	148	8	expres	expre	NOUN
njb-38	148	9	figure	figure	NOUN
njb-38	148	10	8	8	NUM
njb-38	148	11	.	.	PUNCT
njb-38	148	12	effect	effect	NOUN
njb-38	148	13	of	of	ADP
njb-38	148	14	sbiii	sbiii	NOUN
njb-38	148	15	on	on	ADP
njb-38	148	16	hsp70	hsp70	PROPN
njb-38	148	17	protein	protein	NOUN
njb-38	148	18	expression	expression	NOUN
njb-38	148	19	at	at	ADP
njb-38	148	20	different	different	ADJ
njb-38	148	21	time	time	NOUN
njb-38	148	22	points	point	NOUN
njb-38	148	23	.	.	PUNCT
njb-38	149	1	(	(	PUNCT
njb-38	149	2	a	a	X
njb-38	149	3	)	)	PUNCT
njb-38	149	4	western	western	ADJ
njb-38	149	5	blot	blot	NOUN
njb-38	149	6	analysis	analysis	NOUN
njb-38	149	7	of	of	ADP
njb-38	149	8	hsp70	hsp70	NOUN
njb-38	149	9	in	in	ADP
njb-38	149	10	l.	l.	PROPN
njb-38	149	11	donovani	donovani	PROPN
njb-38	149	12	promastigotes	promastigote	NOUN
njb-38	149	13	after	after	ADP
njb-38	149	14	treatment	treatment	NOUN
njb-38	149	15	with	with	ADP
njb-38	149	16	15	15	NUM
njb-38	149	17	µm	µm	NOUN
njb-38	149	18	sbiii	sbiii	NOUN
njb-38	149	19	for	for	ADP
njb-38	149	20	0	0	NUM
njb-38	149	21	h	h	NUM
njb-38	149	22	,	,	PUNCT
njb-38	149	23	12	12	NUM
njb-38	149	24	h	h	NOUN
njb-38	149	25	and	and	CCONJ
njb-38	149	26	24	24	NUM
njb-38	149	27	h.	h.	NOUN
njb-38	149	28	the	the	DET
njb-38	149	29	same	same	ADJ
njb-38	149	30	blot	blot	NOUN
njb-38	149	31	was	be	AUX
njb-38	149	32	reacted	react	VERB
njb-38	149	33	with	with	ADP
njb-38	149	34	antibody	antibody	NOUN
njb-38	149	35	against	against	ADP
njb-38	149	36	β	β	NOUN
njb-38	149	37	-	-	PUNCT
njb-38	149	38	tubulin	tubulin	NOUN
njb-38	149	39	protein	protein	NOUN
njb-38	149	40	to	to	PART
njb-38	149	41	normalize	normalize	VERB
njb-38	149	42	the	the	DET
njb-38	149	43	loading	loading	NOUN
njb-38	149	44	on	on	ADP
njb-38	149	45	to	to	ADP
njb-38	149	46	each	each	DET
njb-38	149	47	lane	lane	NOUN
njb-38	149	48	of	of	ADP
njb-38	149	49	the	the	DET
njb-38	149	50	gel	gel	NOUN
njb-38	149	51	.	.	PUNCT
njb-38	150	1	(	(	PUNCT
njb-38	150	2	b	b	NOUN
njb-38	150	3	)	)	PUNCT
njb-38	150	4	densitometric	densitometric	ADJ
njb-38	150	5	scanning	scanning	NOUN
njb-38	150	6	of	of	ADP
njb-38	150	7	the	the	DET
njb-38	150	8	western	western	ADJ
njb-38	150	9	blot	blot	NOUN
njb-38	150	10	in	in	ADP
njb-38	150	11	a.	a.	NOUN
njb-38	150	12	the	the	DET
njb-38	150	13	bands	band	NOUN
njb-38	150	14	were	be	AUX
njb-38	150	15	quantified	quantify	VERB
njb-38	150	16	by	by	ADP
njb-38	150	17	scanning	scan	VERB
njb-38	150	18	on	on	ADP
njb-38	150	19	a	a	DET
njb-38	150	20	densitometer	densitometer	NOUN
njb-38	150	21	and	and	CCONJ
njb-38	150	22	fold	fold	ADJ
njb-38	150	23	difference	difference	NOUN
njb-38	150	24	in	in	ADP
njb-38	150	25	signal	signal	ADJ
njb-38	150	26	intensities	intensity	NOUN
njb-38	150	27	relative	relative	ADJ
njb-38	150	28	to	to	ADP
njb-38	150	29	the	the	DET
njb-38	150	30	zero	zero	NUM
njb-38	150	31	control	control	NOUN
njb-38	150	32	were	be	AUX
njb-38	150	33	plotted	plot	VERB
njb-38	150	34	.	.	PUNCT
njb-38	151	1	nepal	nepal	ADJ
njb-38	151	2	journal	journal	PROPN
njb-38	151	3	of	of	ADP
njb-38	151	4	biotechnology	biotechnology	NOUN
njb-38	151	5	.	.	PUNCT
njb-38	152	1	dec	dec	PROPN
njb-38	152	2	.	.	PROPN
njb-38	152	3	2015	2015	NUM
njb-38	152	4	vol	vol	NOUN
njb-38	152	5	.	.	PROPN
njb-38	153	1	3	3	NUM
njb-38	153	2	,	,	PUNCT
njb-38	153	3	no	no	INTJ
njb-38	153	4	.	.	PUNCT
njb-38	154	1	1:22	1:22	NUM
njb-38	154	2	-	-	SYM
njb-38	154	3	28	28	NUM
njb-38	154	4	maharjan	maharjan	NOUN
njb-38	154	5	and	and	CCONJ
njb-38	154	6	madhubala	madhubala	PROPN
njb-38	154	7	©	©	PROPN
njb-38	154	8	njb	njb	PROPN
njb-38	154	9	,	,	PUNCT
njb-38	154	10	biotechnology	biotechnology	NOUN
njb-38	154	11	society	society	NOUN
njb-38	154	12	of	of	ADP
njb-38	154	13	nepal	nepal	ADJ
njb-38	154	14	27	27	NUM
njb-38	154	15	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-38	154	16	expression	expression	NOUN
njb-38	154	17	of	of	ADP
njb-38	154	18	hsp70	hsp70	PROPN
njb-38	154	19	protein	protein	NOUN
njb-38	154	20	in	in	ADP
njb-38	154	21	antimony	antimony	NOUN
njb-38	154	22	resistant	resistant	ADJ
njb-38	154	23	l.	l.	NOUN
njb-38	154	24	tarentolae	tarentolae	PROPN
njb-38	154	25	and	and	CCONJ
njb-38	154	26	l.	l.	PROPN
njb-38	154	27	infantum	infantum	PROPN
njb-38	154	28	laboratory	laboratory	PROPN
njb-38	154	29	mutants	mutant	NOUN
njb-38	154	30	and	and	CCONJ
njb-38	154	31	the	the	DET
njb-38	154	32	increased	increase	VERB
njb-38	154	33	expression	expression	NOUN
njb-38	154	34	of	of	ADP
njb-38	154	35	hsp70	hsp70	NOUN
njb-38	154	36	was	be	AUX
njb-38	154	37	stable	stable	ADJ
njb-38	154	38	after	after	ADP
njb-38	154	39	hundreds	hundred	NOUN
njb-38	154	40	of	of	ADP
njb-38	154	41	passages	passage	NOUN
njb-38	154	42	without	without	ADP
njb-38	154	43	the	the	DET
njb-38	154	44	drug	drug	NOUN
njb-38	154	45	[	[	PUNCT
njb-38	154	46	10	10	NUM
njb-38	154	47	]	]	PUNCT
njb-38	154	48	.	.	PUNCT
njb-38	155	1	if	if	SCONJ
njb-38	155	2	the	the	DET
njb-38	155	3	linkage	linkage	NOUN
njb-38	155	4	between	between	ADP
njb-38	155	5	hsp70	hsp70	PROPN
njb-38	155	6	overexpression	overexpression	NOUN
njb-38	155	7	and	and	CCONJ
njb-38	155	8	antimony	antimony	NOUN
njb-38	155	9	resistant	resistant	ADJ
njb-38	155	10	phenotype	phenotype	NOUN
njb-38	155	11	observed	observe	VERB
njb-38	155	12	in	in	ADP
njb-38	155	13	those	those	DET
njb-38	155	14	laboratory	laboratory	NOUN
njb-38	155	15	mutants	mutant	NOUN
njb-38	155	16	is	be	AUX
njb-38	155	17	also	also	ADV
njb-38	155	18	true	true	ADJ
njb-38	155	19	for	for	ADP
njb-38	155	20	l.	l.	PROPN
njb-38	155	21	donovani	donovani	PROPN
njb-38	155	22	field	field	PROPN
njb-38	155	23	isolates	isolate	NOUN
njb-38	155	24	,	,	PUNCT
njb-38	155	25	hsp70	hsp70	NOUN
njb-38	155	26	gene	gene	NOUN
njb-38	155	27	could	could	AUX
njb-38	155	28	be	be	AUX
njb-38	155	29	one	one	NUM
njb-38	155	30	of	of	ADP
njb-38	155	31	the	the	DET
njb-38	155	32	important	important	ADJ
njb-38	155	33	biomarker	biomarker	NOUN
njb-38	155	34	for	for	ADP
njb-38	155	35	monitoring	monitor	VERB
njb-38	155	36	antimony	antimony	ADJ
njb-38	155	37	resistance	resistance	NOUN
njb-38	155	38	in	in	ADP
njb-38	155	39	the	the	DET
njb-38	155	40	field	field	NOUN
njb-38	155	41	conditions	condition	NOUN
njb-38	155	42	.	.	PUNCT
njb-38	156	1	with	with	ADP
njb-38	156	2	this	this	DET
njb-38	156	3	hypothesis	hypothesis	NOUN
njb-38	156	4	we	we	PRON
njb-38	156	5	have	have	AUX
njb-38	156	6	cloned	clone	VERB
njb-38	156	7	the	the	DET
njb-38	156	8	hsp70	hsp70	PROPN
njb-38	156	9	gene	gene	NOUN
njb-38	156	10	in	in	ADP
njb-38	156	11	bacterial	bacterial	ADJ
njb-38	156	12	expression	expression	NOUN
njb-38	156	13	vector	vector	NOUN
njb-38	156	14	,	,	PUNCT
njb-38	156	15	purified	purify	VERB
njb-38	156	16	the	the	DET
njb-38	156	17	recombinant	recombinant	ADJ
njb-38	156	18	protein	protein	NOUN
njb-38	156	19	and	and	CCONJ
njb-38	156	20	raised	raise	VERB
njb-38	156	21	the	the	DET
njb-38	156	22	polyclonal	polyclonal	ADJ
njb-38	156	23	antibody	antibody	NOUN
njb-38	156	24	against	against	ADP
njb-38	156	25	hsp70	hsp70	PROPN
njb-38	156	26	antigen	antigen	PROPN
njb-38	156	27	in	in	ADP
njb-38	156	28	balb	balb	PROPN
njb-38	156	29	/	/	SYM
njb-38	156	30	c	c	NOUN
njb-38	156	31	mice	mouse	NOUN
njb-38	156	32	.	.	PUNCT
njb-38	157	1	we	we	PRON
njb-38	157	2	checked	check	VERB
njb-38	157	3	the	the	DET
njb-38	157	4	hsp70	hsp70	PROPN
njb-38	157	5	protein	protein	NOUN
njb-38	157	6	expression	expression	NOUN
njb-38	157	7	in	in	ADP
njb-38	157	8	two	two	NUM
njb-38	157	9	antimony	antimony	NOUN
njb-38	157	10	sensitive	sensitive	ADJ
njb-38	157	11	(	(	PUNCT
njb-38	157	12	ag83	ag83	PROPN
njb-38	157	13	-	-	PUNCT
njb-38	157	14	s	s	PROPN
njb-38	157	15	and	and	CCONJ
njb-38	157	16	2001	2001	NUM
njb-38	157	17	-	-	PUNCT
njb-38	157	18	s	s	PROPN
njb-38	157	19	)	)	PUNCT
njb-38	157	20	and	and	CCONJ
njb-38	157	21	three	three	NUM
njb-38	157	22	natural	natural	ADJ
njb-38	157	23	antimony	antimony	NOUN
njb-38	157	24	resistant	resistant	ADJ
njb-38	157	25	(	(	PUNCT
njb-38	157	26	41	41	NUM
njb-38	157	27	-	-	PUNCT
njb-38	157	28	r	r	NOUN
njb-38	157	29	,	,	PUNCT
njb-38	157	30	ck2	ck2	PROPN
njb-38	157	31	-	-	PUNCT
njb-38	157	32	r	r	NOUN
njb-38	157	33	and	and	CCONJ
njb-38	157	34	ns2	ns2	NOUN
njb-38	157	35	-	-	PUNCT
njb-38	157	36	r	r	NOUN
njb-38	157	37	)	)	PUNCT
njb-38	157	38	l.	l.	PROPN
njb-38	157	39	donovani	donovani	PROPN
njb-38	157	40	clinical	clinical	ADJ
njb-38	157	41	isolates	isolate	NOUN
njb-38	157	42	using	use	VERB
njb-38	157	43	western	western	ADJ
njb-38	157	44	blotting	blotting	NOUN
njb-38	157	45	.	.	PUNCT
njb-38	158	1	we	we	PRON
njb-38	158	2	found	find	VERB
njb-38	158	3	constitutive	constitutive	ADJ
njb-38	158	4	expression	expression	NOUN
njb-38	158	5	of	of	ADP
njb-38	158	6	hsp70	hsp70	PROPN
njb-38	158	7	protein	protein	NOUN
njb-38	158	8	in	in	ADP
njb-38	158	9	both	both	PRON
njb-38	158	10	sag	sag	PROPN
njb-38	158	11	-	-	PUNCT
njb-38	158	12	s	s	NOUN
njb-38	158	13	and	and	CCONJ
njb-38	158	14	sag	sag	ADJ
njb-38	158	15	-	-	PUNCT
njb-38	158	16	r	r	NOUN
njb-38	158	17	clinical	clinical	ADJ
njb-38	158	18	isolates	isolate	NOUN
njb-38	158	19	.	.	PUNCT
njb-38	159	1	no	no	DET
njb-38	159	2	difference	difference	NOUN
njb-38	159	3	in	in	ADP
njb-38	159	4	hsp70	hsp70	PROPN
njb-38	159	5	protein	protein	NOUN
njb-38	159	6	expression	expression	NOUN
njb-38	159	7	was	be	AUX
njb-38	159	8	observed	observe	VERB
njb-38	159	9	in	in	ADP
njb-38	159	10	antimony	antimony	NOUN
njb-38	159	11	sensitive	sensitive	ADJ
njb-38	159	12	and	and	CCONJ
njb-38	159	13	resistant	resistant	ADJ
njb-38	159	14	field	field	NOUN
njb-38	159	15	isolates	isolate	NOUN
njb-38	159	16	.	.	PUNCT
njb-38	160	1	further	far	ADV
njb-38	160	2	,	,	PUNCT
njb-38	160	3	we	we	PRON
njb-38	160	4	characterized	characterize	VERB
njb-38	160	5	the	the	DET
njb-38	160	6	hsp70	hsp70	PROPN
njb-38	160	7	gene	gene	NOUN
njb-38	160	8	in	in	ADP
njb-38	160	9	sag	sag	PROPN
njb-38	160	10	-	-	PUNCT
njb-38	160	11	s	s	NOUN
njb-38	160	12	and	and	CCONJ
njb-38	160	13	sag	sag	ADJ
njb-38	160	14	-	-	PUNCT
njb-38	160	15	r	r	NOUN
njb-38	160	16	l.	l.	PROPN
njb-38	160	17	donovani	donovani	PROPN
njb-38	160	18	indian	indian	PROPN
njb-38	160	19	field	field	NOUN
njb-38	160	20	isolates	isolate	VERB
njb-38	160	21	to	to	PART
njb-38	160	22	check	check	VERB
njb-38	160	23	the	the	DET
njb-38	160	24	chromosomal	chromosomal	ADJ
njb-38	160	25	localization	localization	NOUN
njb-38	160	26	and	and	CCONJ
njb-38	160	27	copy	copy	NOUN
njb-38	160	28	number	number	NOUN
njb-38	160	29	of	of	ADP
njb-38	160	30	the	the	DET
njb-38	160	31	gene	gene	NOUN
njb-38	160	32	.	.	PUNCT
njb-38	161	1	the	the	DET
njb-38	161	2	copy	copy	NOUN
njb-38	161	3	number	number	NOUN
njb-38	161	4	of	of	ADP
njb-38	161	5	hsp70	hsp70	NOUN
njb-38	161	6	gene	gene	NOUN
njb-38	161	7	has	have	AUX
njb-38	161	8	been	be	AUX
njb-38	161	9	reported	report	VERB
njb-38	161	10	to	to	PART
njb-38	161	11	be	be	AUX
njb-38	161	12	considerably	considerably	ADV
njb-38	161	13	varied	varied	ADJ
njb-38	161	14	among	among	ADP
njb-38	161	15	different	different	ADJ
njb-38	161	16	trypanosomatids	trypanosomatid	NOUN
njb-38	161	17	such	such	ADJ
njb-38	161	18	as	as	ADP
njb-38	161	19	single	single	ADJ
njb-38	161	20	copy	copy	NOUN
njb-38	161	21	in	in	ADP
njb-38	161	22	l.	l.	PROPN
njb-38	161	23	braziliensis	braziliensis	PROPN
njb-38	161	24	,	,	PUNCT
njb-38	161	25	2	2	NUM
njb-38	161	26	copies	copy	NOUN
njb-38	161	27	in	in	ADP
njb-38	161	28	l.	l.	PROPN
njb-38	161	29	tarentolae	tarentolae	PROPN
njb-38	161	30	,	,	PUNCT
njb-38	161	31	4	4	NUM
njb-38	161	32	copies	copy	NOUN
njb-38	161	33	in	in	ADP
njb-38	161	34	l.	l.	PROPN
njb-38	161	35	major	major	PROPN
njb-38	161	36	,	,	PUNCT
njb-38	161	37	6	6	NUM
njb-38	161	38	copies	copy	NOUN
njb-38	161	39	in	in	ADP
njb-38	161	40	l.	l.	PROPN
njb-38	161	41	infantum	infantum	PROPN
njb-38	161	42	,	,	PUNCT
njb-38	161	43	7	7	NUM
njb-38	161	44	copies	copy	NOUN
njb-38	161	45	in	in	ADP
njb-38	161	46	l.	l.	PROPN
njb-38	161	47	amazonensis	amazonensis	NOUN
njb-38	161	48	and	and	CCONJ
njb-38	161	49	t.	t.	PROPN
njb-38	161	50	cruzi	cruzi	PROPN
njb-38	162	1	[	[	PUNCT
njb-38	162	2	5	5	NUM
njb-38	162	3	,	,	PUNCT
njb-38	162	4	6	6	NUM
njb-38	162	5	,	,	PUNCT
njb-38	162	6	10	10	NUM
njb-38	162	7	,	,	PUNCT
njb-38	162	8	14	14	NUM
njb-38	162	9	,	,	PUNCT
njb-38	162	10	17	17	NUM
njb-38	162	11	,	,	PUNCT
njb-38	162	12	18	18	NUM
njb-38	162	13	]	]	PUNCT
njb-38	162	14	.	.	PUNCT
njb-38	163	1	in	in	ADP
njb-38	163	2	l.	l.	PROPN
njb-38	163	3	donovani	donovani	PROPN
njb-38	163	4	indian	indian	PROPN
njb-38	163	5	field	field	NOUN
njb-38	163	6	isolates	isolate	VERB
njb-38	163	7	,	,	PUNCT
njb-38	163	8	the	the	DET
njb-38	163	9	copy	copy	NOUN
njb-38	163	10	number	number	NOUN
njb-38	163	11	of	of	ADP
njb-38	163	12	hsp70	hsp70	NOUN
njb-38	163	13	gene	gene	NOUN
njb-38	163	14	was	be	AUX
njb-38	163	15	found	find	VERB
njb-38	163	16	to	to	PART
njb-38	163	17	be	be	AUX
njb-38	163	18	single	single	ADJ
njb-38	163	19	copy	copy	NOUN
njb-38	163	20	.	.	PUNCT
njb-38	164	1	no	no	DET
njb-38	164	2	difference	difference	NOUN
njb-38	164	3	in	in	ADP
njb-38	164	4	gene	gene	NOUN
njb-38	164	5	copy	copy	NOUN
njb-38	164	6	number	number	NOUN
njb-38	164	7	was	be	AUX
njb-38	164	8	observed	observe	VERB
njb-38	164	9	in	in	ADP
njb-38	164	10	between	between	ADP
njb-38	164	11	sag	sag	PROPN
njb-38	164	12	-	-	PUNCT
njb-38	164	13	s	s	NOUN
njb-38	164	14	and	and	CCONJ
njb-38	164	15	sag	sag	ADJ
njb-38	164	16	-	-	PUNCT
njb-38	164	17	r	r	NOUN
njb-38	164	18	field	field	NOUN
njb-38	164	19	isolates	isolate	NOUN
njb-38	164	20	.	.	PUNCT
njb-38	165	1	to	to	PART
njb-38	165	2	further	far	ADV
njb-38	165	3	verify	verify	VERB
njb-38	165	4	the	the	DET
njb-38	165	5	hsp70	hsp70	PROPN
njb-38	165	6	gene	gene	NOUN
njb-38	165	7	amplification	amplification	NOUN
njb-38	165	8	,	,	PUNCT
njb-38	165	9	we	we	PRON
njb-38	165	10	performed	perform	VERB
njb-38	165	11	southern	southern	ADJ
njb-38	165	12	blot	blot	NOUN
njb-38	165	13	hybridization	hybridization	NOUN
njb-38	165	14	of	of	ADP
njb-38	165	15	pfge	pfge	NOUN
njb-38	165	16	blot	blot	NOUN
njb-38	165	17	.	.	PUNCT
njb-38	166	1	hsp70	hsp70	PROPN
njb-38	166	2	probe	probe	VERB
njb-38	166	3	hybridized	hybridize	VERB
njb-38	166	4	to	to	ADP
njb-38	166	5	a	a	DET
njb-38	166	6	highly	highly	ADV
njb-38	166	7	amplified	amplify	VERB
njb-38	166	8	single	single	ADJ
njb-38	166	9	band	band	NOUN
njb-38	166	10	at	at	ADP
njb-38	166	11	the	the	DET
njb-38	166	12	1.2	1.2	NUM
njb-38	166	13	mb	mb	ADP
njb-38	166	14	region	region	NOUN
njb-38	166	15	which	which	PRON
njb-38	166	16	corresponds	correspond	VERB
njb-38	166	17	to	to	ADP
njb-38	166	18	the	the	DET
njb-38	166	19	28	28	NUM
njb-38	166	20	chromosome	chromosome	NOUN
njb-38	166	21	of	of	ADP
njb-38	166	22	the	the	DET
njb-38	166	23	l.	l.	PROPN
njb-38	166	24	infantum	infantum	PROPN
njb-38	166	25	genome	genome	NOUN
njb-38	166	26	database	database	NOUN
njb-38	166	27	.	.	PUNCT
njb-38	167	1	transcript	transcript	NOUN
njb-38	167	2	level	level	NOUN
njb-38	167	3	of	of	ADP
njb-38	167	4	the	the	DET
njb-38	167	5	hsp70	hsp70	PROPN
njb-38	167	6	gene	gene	NOUN
njb-38	167	7	has	have	AUX
njb-38	167	8	been	be	AUX
njb-38	167	9	shown	show	VERB
njb-38	167	10	to	to	PART
njb-38	167	11	be	be	AUX
njb-38	167	12	transiently	transiently	ADV
njb-38	167	13	induced	induce	VERB
njb-38	167	14	by	by	ADP
njb-38	167	15	environmental	environmental	ADJ
njb-38	167	16	stress	stress	NOUN
njb-38	167	17	such	such	ADJ
njb-38	167	18	as	as	ADP
njb-38	167	19	exposure	exposure	NOUN
njb-38	167	20	to	to	ADP
njb-38	167	21	heat	heat	NOUN
njb-38	167	22	shock	shock	NOUN
njb-38	167	23	or	or	CCONJ
njb-38	167	24	heavy	heavy	ADJ
njb-38	167	25	metals	metal	NOUN
njb-38	167	26	[	[	PUNCT
njb-38	167	27	19	19	NUM
njb-38	167	28	]	]	PUNCT
njb-38	167	29	.	.	PUNCT
njb-38	168	1	during	during	ADP
njb-38	168	2	heat	heat	NOUN
njb-38	168	3	shock	shock	NOUN
njb-38	168	4	,	,	PUNCT
njb-38	168	5	hsp70	hsp70	NOUN
njb-38	168	6	transcription	transcription	NOUN
njb-38	168	7	rate	rate	NOUN
njb-38	168	8	increases	increase	NOUN
njb-38	168	9	rapidly	rapidly	ADV
njb-38	168	10	and	and	CCONJ
njb-38	168	11	then	then	ADV
njb-38	168	12	decline	decline	VERB
njb-38	168	13	slowly	slowly	ADV
njb-38	168	14	[	[	X
njb-38	168	15	20	20	NUM
njb-38	168	16	]	]	PUNCT
njb-38	168	17	.	.	PUNCT
njb-38	169	1	increased	increase	VERB
njb-38	169	2	hsp70	hsp70	PROPN
njb-38	169	3	transcript	transcript	NOUN
njb-38	169	4	level	level	NOUN
njb-38	169	5	has	have	AUX
njb-38	169	6	been	be	AUX
njb-38	169	7	shown	show	VERB
njb-38	169	8	in	in	ADP
njb-38	169	9	leishmania	leishmania	PROPN
njb-38	169	10	when	when	SCONJ
njb-38	169	11	cells	cell	NOUN
njb-38	169	12	were	be	AUX
njb-38	169	13	exposed	expose	VERB
njb-38	169	14	to	to	PART
njb-38	169	15	heat	heat	NOUN
njb-38	169	16	[	[	PUNCT
njb-38	169	17	6	6	NUM
njb-38	169	18	]	]	PUNCT
njb-38	169	19	.	.	PUNCT
njb-38	170	1	the	the	DET
njb-38	170	2	transcription	transcription	NOUN
njb-38	170	3	mechanism	mechanism	NOUN
njb-38	170	4	has	have	AUX
njb-38	170	5	shown	show	VERB
njb-38	170	6	to	to	PART
njb-38	170	7	be	be	AUX
njb-38	170	8	controlled	control	VERB
njb-38	170	9	at	at	ADP
njb-38	170	10	the	the	DET
njb-38	170	11	posttranscriptional	posttranscriptional	ADJ
njb-38	170	12	level	level	NOUN
njb-38	170	13	specifically	specifically	ADV
njb-38	170	14	by	by	ADP
njb-38	170	15	the	the	DET
njb-38	170	16	3’untranslated	3’untranslated	NUM
njb-38	170	17	region	region	NOUN
njb-38	170	18	of	of	ADP
njb-38	170	19	the	the	DET
njb-38	170	20	gene	gene	NOUN
njb-38	170	21	in	in	ADP
njb-38	170	22	contrast	contrast	NOUN
njb-38	170	23	to	to	ADP
njb-38	170	24	most	most	ADJ
njb-38	170	25	other	other	ADJ
njb-38	170	26	organisms	organism	NOUN
njb-38	170	27	[	[	PUNCT
njb-38	170	28	7	7	NUM
njb-38	170	29	,	,	PUNCT
njb-38	170	30	21	21	NUM
njb-38	170	31	]	]	PUNCT
njb-38	170	32	.	.	PUNCT
njb-38	171	1	similar	similar	ADJ
njb-38	171	2	regulation	regulation	NOUN
njb-38	171	3	has	have	AUX
njb-38	171	4	also	also	ADV
njb-38	171	5	been	be	AUX
njb-38	171	6	described	describe	VERB
njb-38	171	7	in	in	ADP
njb-38	171	8	the	the	DET
njb-38	171	9	related	relate	VERB
njb-38	171	10	parasite	parasite	NOUN
njb-38	171	11	t.	t.	PROPN
njb-38	171	12	brucei	brucei	NOUN
njb-38	171	13	[	[	PUNCT
njb-38	171	14	5	5	NUM
njb-38	171	15	]	]	X
njb-38	171	16	.	.	PUNCT
njb-38	172	1	increased	increase	VERB
njb-38	172	2	expression	expression	NOUN
njb-38	172	3	of	of	ADP
njb-38	172	4	hsp70	hsp70	NOUN
njb-38	172	5	has	have	AUX
njb-38	172	6	been	be	AUX
njb-38	172	7	reported	report	VERB
njb-38	172	8	in	in	ADP
njb-38	172	9	antimony	antimony	NOUN
njb-38	172	10	resistant	resistant	ADJ
njb-38	172	11	l.	l.	PROPN
njb-38	172	12	tarentolae	tarentolae	PROPN
njb-38	172	13	and	and	CCONJ
njb-38	172	14	l.	l.	PROPN
njb-38	172	15	infantum	infantum	PROPN
njb-38	172	16	mutants	mutant	NOUN
njb-38	172	17	compared	compare	VERB
njb-38	172	18	to	to	ADP
njb-38	172	19	wild	wild	ADJ
njb-38	172	20	type	type	NOUN
njb-38	172	21	cells	cell	NOUN
njb-38	172	22	[	[	PUNCT
njb-38	172	23	10	10	NUM
njb-38	172	24	]	]	PUNCT
njb-38	172	25	.	.	PUNCT
njb-38	173	1	the	the	DET
njb-38	173	2	increased	increase	VERB
njb-38	173	3	expressions	expression	NOUN
njb-38	173	4	of	of	ADP
njb-38	173	5	hsp70	hsp70	NOUN
njb-38	173	6	in	in	ADP
njb-38	173	7	laboratory	laboratory	NOUN
njb-38	173	8	mutant	mutant	NOUN
njb-38	173	9	were	be	AUX
njb-38	173	10	found	find	VERB
njb-38	173	11	to	to	PART
njb-38	173	12	be	be	AUX
njb-38	173	13	stable	stable	ADJ
njb-38	173	14	after	after	ADP
njb-38	173	15	hundreds	hundred	NOUN
njb-38	173	16	of	of	ADP
njb-38	173	17	passages	passage	NOUN
njb-38	173	18	without	without	ADP
njb-38	173	19	the	the	DET
njb-38	173	20	drug	drug	NOUN
njb-38	173	21	[	[	PUNCT
njb-38	173	22	10	10	NUM
njb-38	173	23	]	]	PUNCT
njb-38	173	24	.	.	PUNCT
njb-38	174	1	earlier	early	ADJ
njb-38	174	2	studies	study	NOUN
njb-38	174	3	indicated	indicate	VERB
njb-38	174	4	that	that	SCONJ
njb-38	174	5	hsp70	hsp70	PROPN
njb-38	174	6	overexpression	overexpression	NOUN
njb-38	174	7	in	in	ADP
njb-38	174	8	antimony	antimony	ADJ
njb-38	174	9	resistant	resistant	ADJ
njb-38	174	10	mutants	mutant	NOUN
njb-38	174	11	was	be	AUX
njb-38	174	12	the	the	DET
njb-38	174	13	stable	stable	ADJ
njb-38	174	14	phenotype	phenotype	NOUN
njb-38	174	15	.	.	PUNCT
njb-38	175	1	to	to	PART
njb-38	175	2	check	check	VERB
njb-38	175	3	whether	whether	SCONJ
njb-38	175	4	similar	similar	ADJ
njb-38	175	5	mechanism	mechanism	NOUN
njb-38	175	6	is	be	AUX
njb-38	175	7	operating	operate	VERB
njb-38	175	8	in	in	ADP
njb-38	175	9	field	field	NOUN
njb-38	175	10	condition	condition	NOUN
njb-38	175	11	,	,	PUNCT
njb-38	175	12	we	we	PRON
njb-38	175	13	performed	perform	VERB
njb-38	175	14	northern	northern	ADJ
njb-38	175	15	blot	blot	NOUN
njb-38	175	16	hybridization	hybridization	NOUN
njb-38	175	17	of	of	ADP
njb-38	175	18	hsp70	hsp70	PROPN
njb-38	175	19	gene	gene	NOUN
njb-38	175	20	in	in	ADP
njb-38	175	21	between	between	ADP
njb-38	175	22	natural	natural	ADJ
njb-38	175	23	antimony	antimony	NOUN
njb-38	175	24	resistant	resistant	ADJ
njb-38	175	25	l.	l.	PROPN
njb-38	175	26	donovani	donovani	PROPN
njb-38	175	27	clinical	clinical	ADJ
njb-38	175	28	isolates	isolate	NOUN
njb-38	175	29	to	to	ADP
njb-38	175	30	that	that	PRON
njb-38	175	31	of	of	ADP
njb-38	175	32	the	the	DET
njb-38	175	33	antimony	antimony	NOUN
njb-38	175	34	sensitive	sensitive	ADJ
njb-38	175	35	parasites	parasite	NOUN
njb-38	175	36	.	.	PUNCT
njb-38	176	1	no	no	DET
njb-38	176	2	difference	difference	NOUN
njb-38	176	3	in	in	ADP
njb-38	176	4	hsp70	hsp70	PROPN
njb-38	176	5	transcript	transcript	NOUN
njb-38	176	6	level	level	NOUN
njb-38	176	7	was	be	AUX
njb-38	176	8	found	find	VERB
njb-38	176	9	between	between	ADP
njb-38	176	10	sag	sag	PROPN
njb-38	176	11	-	-	PUNCT
njb-38	176	12	s	s	NOUN
njb-38	176	13	and	and	CCONJ
njb-38	176	14	sag	sag	ADJ
njb-38	176	15	-	-	PUNCT
njb-38	176	16	r	r	NOUN
njb-38	176	17	clinical	clinical	ADJ
njb-38	176	18	isolates	isolate	NOUN
njb-38	176	19	.	.	PUNCT
njb-38	177	1	arsenite	arsenite	NOUN
njb-38	177	2	and	and	CCONJ
njb-38	177	3	sb	sb	PROPN
njb-38	177	4	iii	iii	PROPN
njb-38	177	5	have	have	AUX
njb-38	177	6	been	be	AUX
njb-38	177	7	described	describe	VERB
njb-38	177	8	as	as	ADP
njb-38	177	9	the	the	DET
njb-38	177	10	known	know	VERB
njb-38	177	11	inducer	inducer	NOUN
njb-38	177	12	of	of	ADP
njb-38	177	13	the	the	DET
njb-38	177	14	hsp70	hsp70	NOUN
njb-38	177	15	in	in	ADP
njb-38	177	16	leishmania	leishmania	PROPN
njb-38	178	1	[	[	PUNCT
njb-38	178	2	10	10	NUM
njb-38	178	3	,	,	PUNCT
njb-38	178	4	22	22	NUM
njb-38	178	5	,	,	PUNCT
njb-38	178	6	23	23	NUM
njb-38	178	7	]	]	PUNCT
njb-38	178	8	.	.	PUNCT
njb-38	179	1	by	by	ADP
njb-38	179	2	gene	gene	NOUN
njb-38	179	3	transfection	transfection	NOUN
njb-38	179	4	experiment	experiment	NOUN
njb-38	179	5	,	,	PUNCT
njb-38	179	6	it	it	PRON
njb-38	179	7	has	have	AUX
njb-38	179	8	been	be	AUX
njb-38	179	9	shown	show	VERB
njb-38	179	10	that	that	SCONJ
njb-38	179	11	hsp70	hsp70	PROPN
njb-38	179	12	contribute	contribute	VERB
njb-38	179	13	first	first	ADJ
njb-38	179	14	line	line	NOUN
njb-38	179	15	of	of	ADP
njb-38	179	16	defense	defense	NOUN
njb-38	179	17	against	against	ADP
njb-38	179	18	sb	sb	PROPN
njb-38	179	19	iii	iii	PUNCT
njb-38	180	1	[	[	PUNCT
njb-38	180	2	10	10	NUM
njb-38	180	3	]	]	PUNCT
njb-38	180	4	suggesting	suggest	VERB
njb-38	180	5	the	the	DET
njb-38	180	6	role	role	NOUN
njb-38	180	7	of	of	ADP
njb-38	180	8	hsp70	hsp70	NOUN
njb-38	180	9	in	in	ADP
njb-38	180	10	antimonial	antimonial	ADJ
njb-38	180	11	drug	drug	NOUN
njb-38	180	12	resistance	resistance	NOUN
njb-38	180	13	.	.	PUNCT
njb-38	181	1	in	in	ADP
njb-38	181	2	the	the	DET
njb-38	181	3	present	present	ADJ
njb-38	181	4	study	study	NOUN
njb-38	181	5	,	,	PUNCT
njb-38	181	6	we	we	PRON
njb-38	181	7	checked	check	VERB
njb-38	181	8	hsp70	hsp70	NOUN
njb-38	181	9	protein	protein	NOUN
njb-38	181	10	expression	expression	NOUN
njb-38	181	11	in	in	ADP
njb-38	181	12	promastigotes	promastigote	NOUN
njb-38	181	13	of	of	ADP
njb-38	181	14	l.	l.	PROPN
njb-38	181	15	donovani	donovani	PROPN
njb-38	181	16	(	(	PUNCT
njb-38	181	17	ag83	ag83	PROPN
njb-38	181	18	-	-	PUNCT
njb-38	181	19	s	s	PROPN
njb-38	181	20	)	)	PUNCT
njb-38	181	21	in	in	ADP
njb-38	181	22	presence	presence	NOUN
njb-38	181	23	of	of	ADP
njb-38	181	24	sb	sb	PROPN
njb-38	181	25	iii	iii	PROPN
njb-38	181	26	.	.	PUNCT
njb-38	182	1	interestingly	interestingly	ADV
njb-38	182	2	,	,	PUNCT
njb-38	182	3	we	we	PRON
njb-38	182	4	could	could	AUX
njb-38	182	5	find	find	VERB
njb-38	182	6	considerable	considerable	ADJ
njb-38	182	7	over	over	ADP
njb-38	182	8	expression	expression	NOUN
njb-38	182	9	of	of	ADP
njb-38	182	10	hsp70	hsp70	PROPN
njb-38	182	11	protein	protein	NOUN
njb-38	182	12	when	when	SCONJ
njb-38	182	13	cells	cell	NOUN
njb-38	182	14	were	be	AUX
njb-38	182	15	incubated	incubate	VERB
njb-38	182	16	in	in	ADP
njb-38	182	17	ic50	ic50	PROPN
njb-38	182	18	concentration	concentration	NOUN
njb-38	182	19	of	of	ADP
njb-38	182	20	sb	sb	PROPN
njb-38	182	21	iii	iii	PROPN
njb-38	182	22	.	.	PROPN
njb-38	182	23	protein	protein	NOUN
njb-38	182	24	over	over	ADP
njb-38	182	25	expression	expression	NOUN
njb-38	182	26	was	be	AUX
njb-38	182	27	about	about	ADV
njb-38	182	28	1.7	1.7	NUM
njb-38	182	29	fold	fold	NOUN
njb-38	182	30	at	at	ADP
njb-38	182	31	12	12	NUM
njb-38	182	32	hours	hour	NOUN
njb-38	182	33	that	that	PRON
njb-38	182	34	increased	increase	VERB
njb-38	182	35	up	up	ADV
njb-38	182	36	to	to	ADP
njb-38	182	37	3	3	NUM
njb-38	182	38	fold	fold	NOUN
njb-38	182	39	at	at	ADP
njb-38	182	40	24	24	NUM
njb-38	182	41	hours	hour	NOUN
njb-38	182	42	time	time	NOUN
njb-38	182	43	period	period	NOUN
njb-38	182	44	.	.	PUNCT
njb-38	183	1	the	the	DET
njb-38	183	2	observed	observe	VERB
njb-38	183	3	results	result	NOUN
njb-38	183	4	suggest	suggest	VERB
njb-38	183	5	that	that	SCONJ
njb-38	183	6	leishmania	leishmania	PROPN
njb-38	183	7	cells	cell	NOUN
njb-38	183	8	respond	respond	VERB
njb-38	183	9	to	to	ADP
njb-38	183	10	antimonial	antimonial	ADJ
njb-38	183	11	drug	drug	NOUN
njb-38	183	12	stress	stress	NOUN
njb-38	183	13	by	by	ADP
njb-38	183	14	over	over	ADP
njb-38	183	15	expression	expression	NOUN
njb-38	183	16	of	of	ADP
njb-38	183	17	the	the	DET
njb-38	183	18	hsp70	hsp70	PROPN
njb-38	183	19	protein	protein	NOUN
njb-38	183	20	.	.	PUNCT
njb-38	184	1	acknowledgements	acknowledgement	VERB
njb-38	184	2	the	the	DET
njb-38	184	3	authors	author	NOUN
njb-38	184	4	thank	thank	VERB
njb-38	184	5	university	university	PROPN
njb-38	184	6	grants	grants	PROPN
njb-38	184	7	commission	commission	PROPN
njb-38	184	8	,	,	PUNCT
njb-38	184	9	new	new	PROPN
njb-38	184	10	delhi	delhi	PROPN
njb-38	184	11	,	,	PUNCT
njb-38	184	12	india	india	PROPN
njb-38	184	13	for	for	ADP
njb-38	184	14	providing	provide	VERB
njb-38	184	15	jrf	jrf	PROPN
njb-38	184	16	/	/	SYM
njb-38	184	17	srf	srf	PROPN
njb-38	184	18	to	to	ADP
njb-38	184	19	mahendra	mahendra	PROPN
njb-38	184	20	maharjan	maharjan	PROPN
njb-38	184	21	to	to	PART
njb-38	184	22	conduct	conduct	VERB
njb-38	184	23	this	this	DET
njb-38	184	24	study	study	NOUN
njb-38	184	25	.	.	PUNCT
njb-38	185	1	references	reference	NOUN
njb-38	185	2	1	1	NUM
njb-38	185	3	.	.	PUNCT
njb-38	186	1	desjeux	desjeux	PROPN
njb-38	186	2	p	p	PROPN
njb-38	186	3	:	:	PUNCT
njb-38	186	4	the	the	DET
njb-38	186	5	increase	increase	NOUN
njb-38	186	6	in	in	ADP
njb-38	186	7	risk	risk	NOUN
njb-38	186	8	factors	factor	NOUN
njb-38	186	9	for	for	ADP
njb-38	186	10	leishmaniasis	leishmaniasis	NOUN
njb-38	186	11	worldwide	worldwide	ADV
njb-38	186	12	.	.	PUNCT
njb-38	187	1	trans	trans	PROPN
njb-38	187	2	r	r	PROPN
njb-38	187	3	soc	soc	PROPN
njb-38	187	4	trop	trop	PROPN
njb-38	187	5	med	me	VERB
njb-38	187	6	hyg	hyg	PROPN
njb-38	187	7	2001	2001	NUM
njb-38	187	8	,	,	PUNCT
njb-38	187	9	95	95	NUM
njb-38	187	10	:	:	PUNCT
njb-38	187	11	239	239	NUM
njb-38	187	12	-	-	SYM
njb-38	187	13	243	243	NUM
njb-38	187	14	.	.	NUM
njb-38	187	15	2	2	NUM
njb-38	187	16	.	.	PUNCT
njb-38	187	17	desjeux	desjeux	PROPN
njb-38	187	18	p	p	PROPN
njb-38	187	19	:	:	PUNCT
njb-38	187	20	leishmaniasis	leishmaniasis	NOUN
njb-38	187	21	:	:	PUNCT
njb-38	187	22	current	current	ADJ
njb-38	187	23	situation	situation	NOUN
njb-38	187	24	and	and	CCONJ
njb-38	187	25	new	new	ADJ
njb-38	187	26	perspectives	perspective	NOUN
njb-38	187	27	.	.	PUNCT
njb-38	188	1	comp	comp	NOUN
njb-38	188	2	immunol	immunol	NOUN
njb-38	188	3	microbiol	microbiol	PROPN
njb-38	188	4	infect	infect	PROPN
njb-38	188	5	dis	dis	PROPN
njb-38	188	6	2004	2004	NUM
njb-38	188	7	,	,	PUNCT
njb-38	188	8	27	27	NUM
njb-38	188	9	:	:	PUNCT
njb-38	188	10	305	305	NUM
njb-38	188	11	-	-	SYM
njb-38	188	12	318	318	NUM
njb-38	188	13	.	.	NUM
njb-38	189	1	3	3	X
njb-38	189	2	.	.	PUNCT
njb-38	189	3	brandonisio	brandonisio	PROPN
njb-38	189	4	o	o	NOUN
njb-38	189	5	and	and	CCONJ
njb-38	189	6	spinelli	spinelli	NOUN
njb-38	189	7	r	r	NOUN
njb-38	189	8	:	:	PUNCT
njb-38	189	9	immune	immune	ADJ
njb-38	189	10	response	response	NOUN
njb-38	189	11	to	to	ADP
njb-38	189	12	parasitic	parasitic	ADJ
njb-38	189	13	infections	infection	NOUN
njb-38	189	14	--	--	PUNCT
njb-38	189	15	an	an	DET
njb-38	189	16	introduction	introduction	NOUN
njb-38	189	17	.	.	PUNCT
njb-38	190	1	curr	curr	PROPN
njb-38	190	2	drug	drug	PROPN
njb-38	190	3	target	target	PROPN
njb-38	190	4	.	.	PUNCT
njb-38	191	1	immune	immune	ADJ
njb-38	191	2	endocr	endocr	PROPN
njb-38	191	3	metabol	metabol	PROPN
njb-38	191	4	disord	disord	PROPN
njb-38	191	5	2002	2002	NUM
njb-38	191	6	,	,	PUNCT
njb-38	191	7	2	2	NUM
njb-38	191	8	:	:	SYM
njb-38	191	9	193199	193199	NUM
njb-38	191	10	.	.	PUNCT
njb-38	192	1	4	4	X
njb-38	192	2	.	.	X
njb-38	192	3	pockley	pockley	PROPN
njb-38	192	4	ag	ag	PROPN
njb-38	192	5	:	:	PUNCT
njb-38	192	6	heat	heat	NOUN
njb-38	192	7	shock	shock	NOUN
njb-38	192	8	proteins	protein	NOUN
njb-38	192	9	in	in	ADP
njb-38	192	10	health	health	NOUN
njb-38	192	11	and	and	CCONJ
njb-38	192	12	disease	disease	NOUN
njb-38	192	13	:	:	PUNCT
njb-38	192	14	therapeutic	therapeutic	ADJ
njb-38	192	15	targets	target	NOUN
njb-38	192	16	or	or	CCONJ
njb-38	192	17	therapeutic	therapeutic	ADJ
njb-38	192	18	agents	agent	NOUN
njb-38	192	19	?	?	PUNCT
njb-38	193	1	expert	expert	NOUN
njb-38	193	2	rev	rev	PROPN
njb-38	193	3	mol	mol	PROPN
njb-38	193	4	med	me	VERB
njb-38	193	5	2001	2001	NUM
njb-38	193	6	,	,	PUNCT
njb-38	193	7	3	3	NUM
njb-38	193	8	:	:	SYM
njb-38	193	9	1	1	NUM
njb-38	193	10	-	-	SYM
njb-38	193	11	21	21	NUM
njb-38	193	12	.	.	PUNCT
njb-38	193	13	nepal	nepal	ADJ
njb-38	193	14	journal	journal	PROPN
njb-38	193	15	of	of	ADP
njb-38	193	16	biotechnology	biotechnology	NOUN
njb-38	193	17	.	.	PUNCT
njb-38	194	1	dec	dec	PROPN
njb-38	194	2	.	.	PROPN
njb-38	194	3	2015	2015	NUM
njb-38	194	4	vol	vol	NOUN
njb-38	194	5	.	.	PROPN
njb-38	195	1	3	3	NUM
njb-38	195	2	,	,	PUNCT
njb-38	195	3	no	no	INTJ
njb-38	195	4	.	.	PUNCT
njb-38	196	1	1:22	1:22	NUM
njb-38	196	2	-	-	SYM
njb-38	196	3	28	28	NUM
njb-38	196	4	maharjan	maharjan	NOUN
njb-38	196	5	and	and	CCONJ
njb-38	196	6	madhubala	madhubala	PROPN
njb-38	196	7	©	©	PROPN
njb-38	196	8	njb	njb	PROPN
njb-38	196	9	,	,	PUNCT
njb-38	196	10	biotechnology	biotechnology	NOUN
njb-38	196	11	society	society	NOUN
njb-38	196	12	of	of	ADP
njb-38	196	13	nepal	nepal	ADJ
njb-38	196	14	28	28	NUM
njb-38	196	15	nepjol.info/index.php/njb	nepjol.info/index.php/njb	PROPN
njb-38	196	16	5	5	NUM
njb-38	196	17	.	.	PUNCT
njb-38	197	1	lee	lee	PROPN
njb-38	197	2	mg	mg	PROPN
njb-38	197	3	,	,	PUNCT
njb-38	197	4	atkinson	atkinson	PROPN
njb-38	197	5	bl	bl	PROPN
njb-38	197	6	,	,	PUNCT
njb-38	197	7	giannini	giannini	PROPN
njb-38	197	8	sh	sh	PROPN
njb-38	197	9	,	,	PUNCT
njb-38	197	10	van	van	PROPN
njb-38	197	11	der	der	PROPN
njb-38	197	12	ploeg	ploeg	PROPN
njb-38	197	13	lh	lh	PROPN
njb-38	197	14	:	:	PUNCT
njb-38	197	15	structure	structure	NOUN
njb-38	197	16	and	and	CCONJ
njb-38	197	17	expression	expression	NOUN
njb-38	197	18	of	of	ADP
njb-38	197	19	the	the	DET
njb-38	197	20	hsp	hsp	ADJ
njb-38	197	21	70	70	NUM
njb-38	197	22	gene	gene	NOUN
njb-38	197	23	family	family	NOUN
njb-38	197	24	of	of	ADP
njb-38	197	25	leishmania	leishmania	PROPN
njb-38	197	26	major	major	PROPN
njb-38	197	27	.	.	PUNCT
njb-38	198	1	nucleic	nucleic	ADJ
njb-38	198	2	acids	acids	PROPN
njb-38	198	3	research	research	PROPN
njb-38	198	4	,	,	PUNCT
njb-38	198	5	1988	1988	NUM
njb-38	198	6	,	,	PUNCT
njb-38	198	7	16(20):9567	16(20):9567	NUM
njb-38	198	8	-	-	SYM
njb-38	198	9	9585	9585	NUM
njb-38	198	10	.	.	PUNCT
njb-38	199	1	6	6	NUM
njb-38	199	2	.	.	PUNCT
njb-38	200	1	quijada	quijada	PROPN
njb-38	200	2	l	l	PROPN
njb-38	200	3	,	,	PUNCT
njb-38	200	4	soto	soto	PROPN
njb-38	200	5	m	m	PROPN
njb-38	200	6	,	,	PUNCT
njb-38	200	7	alonso	alonso	PROPN
njb-38	200	8	c	c	PROPN
njb-38	200	9	,	,	PUNCT
njb-38	200	10	requena	requena	PROPN
njb-38	200	11	jm	jm	PROPN
njb-38	200	12	:	:	PUNCT
njb-38	200	13	analysis	analysis	NOUN
njb-38	200	14	of	of	ADP
njb-38	200	15	post	post	ADJ
njb-38	200	16	-	-	ADJ
njb-38	200	17	transcriptional	transcriptional	ADJ
njb-38	200	18	regulation	regulation	NOUN
njb-38	200	19	operating	operate	VERB
njb-38	200	20	on	on	ADP
njb-38	200	21	transcription	transcription	NOUN
njb-38	200	22	products	product	NOUN
njb-38	200	23	of	of	ADP
njb-38	200	24	the	the	DET
njb-38	200	25	tandemly	tandemly	ADV
njb-38	200	26	linked	link	VERB
njb-38	200	27	leishmania	leishmania	PROPN
njb-38	200	28	infantum	infantum	PROPN
njb-38	200	29	hsp70	hsp70	PROPN
njb-38	200	30	genes	gene	NOUN
njb-38	200	31	.	.	PUNCT
njb-38	201	1	j	j	PROPN
njb-38	201	2	biol	biol	PROPN
njb-38	201	3	chem	chem	PROPN
njb-38	201	4	1997	1997	NUM
njb-38	201	5	,	,	PUNCT
njb-38	201	6	272(7):4493	272(7):4493	PROPN
njb-38	201	7	-	-	SYM
njb-38	201	8	4499	4499	NUM
njb-38	201	9	.	.	PUNCT
njb-38	202	1	7	7	NUM
njb-38	202	2	.	.	PUNCT
njb-38	202	3	quijada	quijada	PROPN
njb-38	202	4	l	l	PROPN
njb-38	202	5	,	,	PUNCT
njb-38	202	6	soto	soto	PROPN
njb-38	202	7	m	m	PROPN
njb-38	202	8	,	,	PUNCT
njb-38	202	9	alonso	alonso	PROPN
njb-38	202	10	c	c	NOUN
njb-38	202	11	requena	requena	PROPN
njb-38	202	12	jm	jm	PROPN
njb-38	202	13	:	:	PUNCT
njb-38	202	14	identification	identification	NOUN
njb-38	202	15	of	of	ADP
njb-38	202	16	a	a	DET
njb-38	202	17	putative	putative	ADJ
njb-38	202	18	regulatory	regulatory	ADJ
njb-38	202	19	element	element	NOUN
njb-38	202	20	in	in	ADP
njb-38	202	21	the	the	DET
njb-38	202	22	3'-untranslated	3'-untranslated	NUM
njb-38	202	23	region	region	NOUN
njb-38	202	24	that	that	PRON
njb-38	202	25	controls	control	VERB
njb-38	202	26	expression	expression	NOUN
njb-38	202	27	of	of	ADP
njb-38	202	28	hsp70	hsp70	NOUN
njb-38	202	29	in	in	ADP
njb-38	202	30	leishmania	leishmania	PROPN
njb-38	202	31	infantum	infantum	PROPN
njb-38	202	32	.	.	PUNCT
njb-38	203	1	mol	mol	PROPN
njb-38	203	2	biochem	biochem	NOUN
njb-38	203	3	parasitol	parasitol	PROPN
njb-38	203	4	.	.	PUNCT
njb-38	204	1	2000	2000	NUM
njb-38	204	2	,	,	PUNCT
njb-38	204	3	110(1):79	110(1):79	NUM
njb-38	204	4	-	-	SYM
njb-38	204	5	91	91	NUM
njb-38	204	6	.	.	NUM
njb-38	204	7	8	8	NUM
njb-38	204	8	.	.	PUNCT
njb-38	205	1	guerin	guerin	PROPN
njb-38	205	2	pj	pj	PROPN
njb-38	205	3	,	,	PUNCT
njb-38	205	4	olliaro	olliaro	NOUN
njb-38	205	5	p	p	PROPN
njb-38	205	6	,	,	PUNCT
njb-38	205	7	sundar	sundar	PROPN
njb-38	205	8	s	s	PROPN
njb-38	205	9	,	,	PUNCT
njb-38	205	10	boelaert	boelaert	PROPN
njb-38	205	11	m	m	PROPN
njb-38	205	12	,	,	PUNCT
njb-38	205	13	croft	croft	PROPN
njb-38	205	14	sl	sl	PROPN
njb-38	205	15	,	,	PUNCT
njb-38	205	16	desjeux	desjeux	PROPN
njb-38	205	17	p	p	PROPN
njb-38	205	18	,	,	PUNCT
njb-38	205	19	wasunna	wasunna	PROPN
njb-38	205	20	mk	mk	PROPN
njb-38	205	21	,	,	PUNCT
njb-38	205	22	bryceson	bryceson	PROPN
njb-38	205	23	ad	ad	PROPN
njb-38	205	24	:	:	PUNCT
njb-38	205	25	visceral	visceral	ADJ
njb-38	205	26	leishmaniasis	leishmaniasis	NOUN
njb-38	205	27	:	:	PUNCT
njb-38	205	28	current	current	ADJ
njb-38	205	29	status	status	NOUN
njb-38	205	30	of	of	ADP
njb-38	205	31	control	control	NOUN
njb-38	205	32	,	,	PUNCT
njb-38	205	33	diagnosis	diagnosis	NOUN
njb-38	205	34	,	,	PUNCT
njb-38	205	35	and	and	CCONJ
njb-38	205	36	treatment	treatment	NOUN
njb-38	205	37	,	,	PUNCT
njb-38	205	38	and	and	CCONJ
njb-38	205	39	a	a	DET
njb-38	205	40	proposed	propose	VERB
njb-38	205	41	research	research	NOUN
njb-38	205	42	and	and	CCONJ
njb-38	205	43	development	development	NOUN
njb-38	205	44	agenda	agenda	NOUN
njb-38	205	45	.	.	PUNCT
njb-38	206	1	lancet	lancet	PROPN
njb-38	206	2	infectious	infectious	ADJ
njb-38	206	3	diseases	disease	NOUN
njb-38	206	4	,	,	PUNCT
njb-38	206	5	2002	2002	NUM
njb-38	206	6	,	,	PUNCT
njb-38	206	7	2(8):494	2(8):494	NUM
njb-38	206	8	-	-	SYM
njb-38	206	9	501	501	NUM
njb-38	206	10	.	.	NOUN
njb-38	207	1	9	9	NUM
njb-38	207	2	.	.	X
njb-38	207	3	haimeur	haimeur	VERB
njb-38	207	4	a	a	PRON
njb-38	207	5	,	,	PUNCT
njb-38	207	6	brochu	brochu	PROPN
njb-38	207	7	c	c	PROPN
njb-38	207	8	,	,	PUNCT
njb-38	207	9	genest	genest	PROPN
njb-38	207	10	p	p	PROPN
njb-38	207	11	,	,	PUNCT
njb-38	207	12	papadopoulou	papadopoulou	PROPN
njb-38	207	13	b	b	PROPN
njb-38	207	14	,	,	PUNCT
njb-38	207	15	ouellette	ouellette	NOUN
njb-38	207	16	m	m	NOUN
njb-38	207	17	:	:	PUNCT
njb-38	207	18	amplification	amplification	NOUN
njb-38	207	19	of	of	ADP
njb-38	207	20	the	the	DET
njb-38	207	21	abc	abc	PROPN
njb-38	207	22	transporter	transporter	PROPN
njb-38	207	23	gene	gene	NOUN
njb-38	207	24	pgpa	pgpa	NOUN
njb-38	207	25	and	and	CCONJ
njb-38	207	26	increased	increase	VERB
njb-38	207	27	trypanothione	trypanothione	NOUN
njb-38	207	28	levels	level	NOUN
njb-38	207	29	in	in	ADP
njb-38	207	30	potassium	potassium	NOUN
njb-38	207	31	antimonyl	antimonyl	PROPN
njb-38	207	32	tartrate	tartrate	VERB
njb-38	207	33	(	(	PUNCT
njb-38	207	34	sbiii	sbiii	NOUN
njb-38	207	35	)	)	PUNCT
njb-38	207	36	resistant	resistant	ADJ
njb-38	207	37	leishmania	leishmania	PROPN
njb-38	207	38	tarentolae	tarentolae	PROPN
njb-38	207	39	.	.	PUNCT
njb-38	208	1	mol	mol	PROPN
njb-38	208	2	biochem	biochem	NOUN
njb-38	208	3	parasitol	parasitol	PROPN
njb-38	208	4	2000	2000	NUM
njb-38	208	5	,	,	PUNCT
njb-38	208	6	108(1):131	108(1):131	NUM
njb-38	208	7	-	-	SYM
njb-38	208	8	135	135	NUM
njb-38	208	9	.	.	NUM
njb-38	208	10	10	10	NUM
njb-38	208	11	.	.	PUNCT
njb-38	209	1	brochu	brochu	PROPN
njb-38	209	2	c	c	PROPN
njb-38	209	3	,	,	PUNCT
njb-38	209	4	haimeur	haimeur	VERB
njb-38	209	5	a	a	DET
njb-38	209	6	,	,	PUNCT
njb-38	209	7	ouellette	ouellette	NOUN
njb-38	209	8	m	m	PRON
njb-38	209	9	:	:	PUNCT
njb-38	209	10	the	the	DET
njb-38	209	11	heat	heat	NOUN
njb-38	209	12	shock	shock	NOUN
njb-38	209	13	protein	protein	NOUN
njb-38	209	14	hsp70	hsp70	NOUN
njb-38	209	15	and	and	CCONJ
njb-38	209	16	heat	heat	NOUN
njb-38	209	17	shock	shock	NOUN
njb-38	209	18	cognate	cognate	ADJ
njb-38	209	19	protein	protein	NOUN
njb-38	209	20	hsc70	hsc70	NOUN
njb-38	209	21	contribute	contribute	VERB
njb-38	209	22	to	to	ADP
njb-38	209	23	antimony	antimony	NOUN
njb-38	209	24	tolerance	tolerance	NOUN
njb-38	209	25	in	in	ADP
njb-38	209	26	the	the	DET
njb-38	209	27	protozoan	protozoan	NOUN
njb-38	209	28	parasite	parasite	NOUN
njb-38	209	29	leishmania	leishmania	PROPN
njb-38	209	30	.	.	PUNCT
njb-38	210	1	cell	cell	NOUN
njb-38	210	2	stress	stress	NOUN
njb-38	210	3	chaperones	chaperone	NOUN
njb-38	210	4	2004	2004	NUM
njb-38	210	5	,	,	PUNCT
njb-38	210	6	9(3):294	9(3):294	NUM
njb-38	210	7	-	-	SYM
njb-38	210	8	303	303	NUM
njb-38	210	9	.	.	NUM
njb-38	210	10	11	11	NUM
njb-38	210	11	.	.	PUNCT
njb-38	211	1	bhattacharjee	bhattacharjee	PROPN
njb-38	211	2	h	h	PROPN
njb-38	211	3	,	,	PUNCT
njb-38	211	4	carbrey	carbrey	PROPN
njb-38	211	5	j	j	PROPN
njb-38	211	6	,	,	PUNCT
njb-38	211	7	rosen	rosen	PROPN
njb-38	211	8	bp	bp	PROPN
njb-38	211	9	,	,	PUNCT
njb-38	211	10	mukhopadhyay	mukhopadhyay	PROPN
njb-38	211	11	r	r	NOUN
njb-38	211	12	:	:	PUNCT
njb-38	211	13	drug	drug	NOUN
njb-38	211	14	uptake	uptake	NOUN
njb-38	211	15	and	and	CCONJ
njb-38	211	16	pharmacological	pharmacological	ADJ
njb-38	211	17	modulation	modulation	NOUN
njb-38	211	18	of	of	ADP
njb-38	211	19	drug	drug	NOUN
njb-38	211	20	sensitivity	sensitivity	NOUN
njb-38	211	21	in	in	ADP
njb-38	211	22	leukemia	leukemia	NOUN
njb-38	211	23	by	by	ADP
njb-38	211	24	aqp9	aqp9	PROPN
njb-38	211	25	.	.	PUNCT
njb-38	212	1	biochemical	biochemical	ADJ
njb-38	212	2	and	and	CCONJ
njb-38	212	3	biophysical	biophysical	ADJ
njb-38	212	4	research	research	NOUN
njb-38	212	5	communications	communication	NOUN
njb-38	212	6	2004	2004	NUM
njb-38	212	7	,	,	PUNCT
njb-38	212	8	322(3):836	322(3):836	NUM
njb-38	212	9	-	-	SYM
njb-38	212	10	841	841	NUM
njb-38	212	11	.	.	NUM
njb-38	212	12	12	12	NUM
njb-38	212	13	.	.	PUNCT
njb-38	213	1	bradford	bradford	PROPN
njb-38	213	2	mm	mm	PROPN
njb-38	213	3	:	:	PUNCT
njb-38	213	4	a	a	DET
njb-38	213	5	rapid	rapid	ADJ
njb-38	213	6	and	and	CCONJ
njb-38	213	7	sensitive	sensitive	ADJ
njb-38	213	8	method	method	NOUN
njb-38	213	9	for	for	ADP
njb-38	213	10	the	the	DET
njb-38	213	11	quantitation	quantitation	NOUN
njb-38	213	12	of	of	ADP
njb-38	213	13	microgram	microgram	NOUN
njb-38	213	14	quantities	quantity	NOUN
njb-38	213	15	of	of	ADP
njb-38	213	16	protein	protein	NOUN
njb-38	213	17	utilizing	utilize	VERB
njb-38	213	18	the	the	DET
njb-38	213	19	principle	principle	NOUN
njb-38	213	20	of	of	ADP
njb-38	213	21	protein	protein	NOUN
njb-38	213	22	-	-	PUNCT
njb-38	213	23	dye	dye	NOUN
njb-38	213	24	binding	binding	NOUN
njb-38	213	25	.	.	PUNCT
njb-38	214	1	analytical	analytical	PROPN
njb-38	214	2	biochemistry	biochemistry	NOUN
njb-38	214	3	,	,	PUNCT
njb-38	214	4	1976	1976	NUM
njb-38	214	5	,	,	PUNCT
njb-38	214	6	72:248	72:248	NUM
njb-38	214	7	-	-	SYM
njb-38	214	8	25	25	NUM
njb-38	214	9	,	,	PUNCT
njb-38	214	10	.	.	PUNCT
njb-38	215	1	13	13	NUM
njb-38	215	2	.	.	PUNCT
njb-38	215	3	sambrook	sambrook	PROPN
njb-38	215	4	j	j	PROPN
njb-38	215	5	,	,	PUNCT
njb-38	215	6	fritsch	fritsch	PROPN
njb-38	215	7	ef	ef	PROPN
njb-38	215	8	,	,	PUNCT
njb-38	215	9	maniatis	maniatis	PROPN
njb-38	215	10	t	t	PROPN
njb-38	215	11	:	:	PUNCT
njb-38	215	12	molecular	molecular	ADJ
njb-38	215	13	cloning	cloning	NOUN
njb-38	215	14	:	:	PUNCT
njb-38	215	15	a	a	DET
njb-38	215	16	laboratory	laboratory	NOUN
njb-38	215	17	manual	manual	NOUN
njb-38	215	18	,	,	PUNCT
njb-38	215	19	cold	cold	ADJ
njb-38	215	20	spring	spring	NOUN
njb-38	215	21	harbour	harbour	NOUN
njb-38	215	22	press	press	PROPN
njb-38	215	23	,	,	PUNCT
njb-38	215	24	1989	1989	NUM
njb-38	215	25	.	.	PUNCT
njb-38	216	1	14	14	NUM
njb-38	216	2	.	.	PUNCT
njb-38	217	1	zurita	zurita	PROPN
njb-38	217	2	ai	ai	VERB
njb-38	217	3	,	,	PUNCT
njb-38	217	4	rodriguez	rodriguez	NOUN
njb-38	217	5	j	j	PROPN
njb-38	217	6	,	,	PUNCT
njb-38	217	7	pinero	pinero	PROPN
njb-38	217	8	je	je	PROPN
njb-38	217	9	,	,	PUNCT
njb-38	217	10	pacheco	pacheco	PROPN
njb-38	217	11	r	r	PROPN
njb-38	217	12	,	,	PUNCT
njb-38	217	13	carmelo	carmelo	PROPN
njb-38	217	14	e	e	PROPN
njb-38	217	15	,	,	PUNCT
njb-38	217	16	del	del	PROPN
njb-38	217	17	ca	ca	PROPN
njb-38	217	18	,	,	PUNCT
njb-38	217	19	valladares	valladare	VERB
njb-38	217	20	b	b	PROPN
njb-38	217	21	:	:	PUNCT
njb-38	217	22	cloning	cloning	NOUN
njb-38	217	23	and	and	CCONJ
njb-38	217	24	characterization	characterization	NOUN
njb-38	217	25	of	of	ADP
njb-38	217	26	the	the	DET
njb-38	217	27	leishmania	leishmania	PROPN
njb-38	217	28	(	(	PUNCT
njb-38	217	29	viannia	viannia	NOUN
njb-38	217	30	)	)	PUNCT
njb-38	217	31	braziliensis	braziliensis	NOUN
njb-38	217	32	hsp70	hsp70	PROPN
njb-38	217	33	gene	gene	NOUN
njb-38	217	34	,	,	PUNCT
njb-38	217	35	diagnostic	diagnostic	ADJ
njb-38	217	36	use	use	NOUN
njb-38	217	37	of	of	ADP
njb-38	217	38	the	the	DET
njb-38	217	39	cterminal	cterminal	ADJ
njb-38	217	40	fragment	fragment	PROPN
njb-38	217	41	rlb70(513	rlb70(513	PROPN
njb-38	217	42	-	-	NUM
njb-38	217	43	663	663	NUM
njb-38	217	44	)	)	PUNCT
njb-38	217	45	.	.	PUNCT
njb-38	218	1	journal	journal	PROPN
njb-38	218	2	of	of	ADP
njb-38	218	3	parasitology	parasitology	NOUN
njb-38	218	4	2003	2003	NUM
njb-38	218	5	,	,	PUNCT
njb-38	218	6	89(2):372	89(2):372	NUM
njb-38	218	7	-	-	NUM
njb-38	218	8	378	378	NUM
njb-38	218	9	.	.	NOUN
njb-38	218	10	15	15	NUM
njb-38	218	11	.	.	PUNCT
njb-38	219	1	hartl	hartl	PROPN
njb-38	219	2	fu	fu	PROPN
njb-38	219	3	,	,	PUNCT
njb-38	219	4	hayer	hayer	PROPN
njb-38	219	5	-	-	PUNCT
njb-38	219	6	hartl	hartl	PROPN
njb-38	219	7	m	m	PROPN
njb-38	219	8	:	:	PUNCT
njb-38	219	9	molecular	molecular	ADJ
njb-38	219	10	chaperones	chaperone	NOUN
njb-38	219	11	in	in	ADP
njb-38	219	12	the	the	DET
njb-38	219	13	cytosol	cytosol	NOUN
njb-38	219	14	:	:	PUNCT
njb-38	219	15	from	from	ADP
njb-38	219	16	nascent	nascent	ADJ
njb-38	219	17	chain	chain	NOUN
njb-38	219	18	to	to	ADP
njb-38	219	19	folded	fold	VERB
njb-38	219	20	protein	protein	NOUN
njb-38	219	21	.	.	PUNCT
njb-38	220	1	science	science	NOUN
njb-38	220	2	2002	2002	NUM
njb-38	220	3	,	,	PUNCT
njb-38	220	4	295(5561):1852	295(5561):1852	NUM
njb-38	220	5	-	-	PUNCT
njb-38	220	6	1858	1858	NUM
njb-38	220	7	.	.	PUNCT
njb-38	221	1	16	16	NUM
njb-38	221	2	.	.	PUNCT
njb-38	222	1	morimoto	morimoto	PROPN
njb-38	222	2	ri	ri	PROPN
njb-38	222	3	,	,	PUNCT
njb-38	222	4	santoro	santoro	PROPN
njb-38	222	5	mg	mg	PROPN
njb-38	222	6	:	:	PUNCT
njb-38	222	7	stress	stress	NOUN
njb-38	222	8	-	-	PUNCT
njb-38	222	9	inducible	inducible	ADJ
njb-38	222	10	responses	response	NOUN
njb-38	222	11	and	and	CCONJ
njb-38	222	12	heat	heat	NOUN
njb-38	222	13	shock	shock	NOUN
njb-38	222	14	proteins	protein	NOUN
njb-38	222	15	:	:	PUNCT
njb-38	222	16	new	new	ADJ
njb-38	222	17	pharmacologic	pharmacologic	ADJ
njb-38	222	18	targets	target	NOUN
njb-38	222	19	for	for	ADP
njb-38	222	20	cytoprotection	cytoprotection	NOUN
njb-38	222	21	.	.	PUNCT
njb-38	223	1	nature	nature	NOUN
njb-38	223	2	biotechnology	biotechnology	NOUN
njb-38	223	3	1998	1998	NUM
njb-38	223	4	,	,	PUNCT
njb-38	223	5	16(9):833	16(9):833	NOUN
njb-38	223	6	-	-	SYM
njb-38	223	7	838	838	NUM
njb-38	223	8	.	.	NUM
njb-38	223	9	17	17	NUM
njb-38	223	10	.	.	PUNCT
njb-38	224	1	bock	bock	PROPN
njb-38	224	2	jh	jh	PROPN
njb-38	224	3	,	,	PUNCT
njb-38	224	4	langer	langer	ADJ
njb-38	224	5	pj	pj	NOUN
njb-38	224	6	:	:	PUNCT
njb-38	224	7	sequence	sequence	NOUN
njb-38	224	8	and	and	CCONJ
njb-38	224	9	genomic	genomic	NOUN
njb-38	224	10	organization	organization	NOUN
njb-38	224	11	of	of	ADP
njb-38	224	12	the	the	DET
njb-38	224	13	hsp70	hsp70	PROPN
njb-38	224	14	genes	gene	NOUN
njb-38	224	15	of	of	ADP
njb-38	224	16	leishmania	leishmania	PROPN
njb-38	224	17	amazonensis	amazonensis	NOUN
njb-38	224	18	.	.	PUNCT
njb-38	225	1	molecular	molecular	ADJ
njb-38	225	2	and	and	CCONJ
njb-38	225	3	biochemical	biochemical	ADJ
njb-38	225	4	parasitology	parasitology	NOUN
njb-38	225	5	1993	1993	NUM
njb-38	225	6	,	,	PUNCT
njb-38	225	7	62	62	NUM
njb-38	225	8	,	,	PUNCT
njb-38	225	9	no	no	NOUN
njb-38	225	10	.	.	NOUN
njb-38	225	11	2	2	NUM
njb-38	225	12	,	,	PUNCT
njb-38	225	13	pp	pp	ADV
njb-38	225	14	.	.	PUNCT
njb-38	226	1	187	187	NUM
njb-38	226	2	-	-	SYM
njb-38	226	3	197	197	NUM
njb-38	226	4	.	.	NUM
njb-38	226	5	18	18	NUM
njb-38	226	6	.	.	PUNCT
njb-38	227	1	requena	requena	PROPN
njb-38	227	2	jm	jm	PROPN
njb-38	227	3	,	,	PUNCT
njb-38	227	4	lopez	lopez	PROPN
njb-38	227	5	mc	mc	PROPN
njb-38	227	6	,	,	PUNCT
njb-38	227	7	jimenez	jimenez	PROPN
njb-38	227	8	-	-	PUNCT
njb-38	227	9	ruiz	ruiz	NOUN
njb-38	227	10	a	a	PROPN
njb-38	227	11	,	,	PUNCT
njb-38	227	12	de	de	X
njb-38	227	13	la	la	PROPN
njb-38	227	14	torre	torre	PROPN
njb-38	227	15	jc	jc	PROPN
njb-38	227	16	,	,	PUNCT
njb-38	227	17	alonso	alonso	PROPN
njb-38	227	18	c	c	NOUN
njb-38	227	19	:	:	PUNCT
njb-38	227	20	a	a	DET
njb-38	227	21	head	head	NOUN
njb-38	227	22	-	-	PUNCT
njb-38	227	23	to	to	ADP
njb-38	227	24	-	-	PUNCT
njb-38	227	25	tail	tail	NOUN
njb-38	227	26	tandem	tandem	NOUN
njb-38	227	27	organization	organization	NOUN
njb-38	227	28	of	of	ADP
njb-38	227	29	hsp70	hsp70	PROPN
njb-38	227	30	genes	gene	NOUN
njb-38	227	31	in	in	ADP
njb-38	227	32	trypanosoma	trypanosoma	PROPN
njb-38	227	33	cruzi	cruzi	PROPN
njb-38	227	34	.	.	PUNCT
njb-38	228	1	nucleic	nucleic	PROPN
njb-38	228	2	acids	acids	PROPN
njb-38	228	3	research	research	PROPN
njb-38	228	4	,	,	PUNCT
njb-38	228	5	1988	1988	NUM
njb-38	228	6	,	,	PUNCT
njb-38	228	7	16:1393	16:1393	NUM
njb-38	228	8	-	-	SYM
njb-38	228	9	1406	1406	NUM
njb-38	228	10	.	.	PUNCT
njb-38	229	1	19	19	NUM
njb-38	229	2	.	.	PUNCT
njb-38	229	3	milarski	milarski	PROPN
njb-38	229	4	kl	kl	PROPN
njb-38	229	5	,	,	PUNCT
njb-38	229	6	morimoto	morimoto	PROPN
njb-38	229	7	ri	ri	NOUN
njb-38	229	8	:	:	PUNCT
njb-38	229	9	expression	expression	NOUN
njb-38	229	10	of	of	ADP
njb-38	229	11	human	human	NOUN
njb-38	229	12	hsp70	hsp70	NOUN
njb-38	229	13	during	during	ADP
njb-38	229	14	the	the	DET
njb-38	229	15	synthetic	synthetic	ADJ
njb-38	229	16	phase	phase	NOUN
njb-38	229	17	of	of	ADP
njb-38	229	18	the	the	DET
njb-38	229	19	cell	cell	NOUN
njb-38	229	20	cycle	cycle	NOUN
njb-38	229	21	.	.	PUNCT
njb-38	230	1	proceedings	proceeding	NOUN
njb-38	230	2	of	of	ADP
njb-38	230	3	the	the	DET
njb-38	230	4	national	national	PROPN
njb-38	230	5	academy	academy	PROPN
njb-38	230	6	of	of	ADP
njb-38	230	7	sciences	sciences	PROPN
njb-38	230	8	1986	1986	NUM
njb-38	230	9	,	,	PUNCT
njb-38	230	10	83(24):9517	83(24):9517	NUM
njb-38	230	11	-	-	SYM
njb-38	230	12	9521	9521	NUM
njb-38	230	13	.	.	PUNCT
njb-38	231	1	20	20	NUM
njb-38	231	2	.	.	PUNCT
njb-38	231	3	theodorakis	theodorakis	PROPN
njb-38	231	4	ng	ng	PROPN
njb-38	231	5	,	,	PUNCT
njb-38	231	6	morimoto	morimoto	PROPN
njb-38	231	7	ri	ri	PROPN
njb-38	231	8	:	:	PUNCT
njb-38	231	9	posttranscriptional	posttranscriptional	ADJ
njb-38	231	10	regulation	regulation	NOUN
njb-38	231	11	of	of	ADP
njb-38	231	12	hsp70	hsp70	PROPN
njb-38	231	13	expression	expression	NOUN
njb-38	231	14	in	in	ADP
njb-38	231	15	human	human	ADJ
njb-38	231	16	cells	cell	NOUN
njb-38	231	17	:	:	PUNCT
njb-38	231	18	effects	effect	NOUN
njb-38	231	19	of	of	ADP
njb-38	231	20	heat	heat	NOUN
njb-38	231	21	shock	shock	NOUN
njb-38	231	22	,	,	PUNCT
njb-38	231	23	inhibition	inhibition	NOUN
njb-38	231	24	of	of	ADP
njb-38	231	25	protein	protein	NOUN
njb-38	231	26	synthesis	synthesis	NOUN
njb-38	231	27	,	,	PUNCT
njb-38	231	28	and	and	CCONJ
njb-38	231	29	adenovirus	adenovirus	NOUN
njb-38	231	30	infection	infection	NOUN
njb-38	231	31	on	on	ADP
njb-38	231	32	translation	translation	NOUN
njb-38	231	33	and	and	CCONJ
njb-38	231	34	mrna	mrna	NOUN
njb-38	231	35	stability	stability	NOUN
njb-38	231	36	.	.	PUNCT
njb-38	232	1	molecular	molecular	ADJ
njb-38	232	2	and	and	CCONJ
njb-38	232	3	cellular	cellular	ADJ
njb-38	232	4	biology	biology	NOUN
njb-38	232	5	,	,	PUNCT
njb-38	232	6	1987	1987	NUM
njb-38	232	7	,	,	PUNCT
njb-38	232	8	7(7):4357	7(7):4357	NOUN
njb-38	232	9	-	-	PUNCT
njb-38	232	10	4368	4368	NUM
njb-38	232	11	.	.	PUNCT
njb-38	233	1	21	21	NUM
njb-38	233	2	.	.	PUNCT
njb-38	233	3	brandau	brandau	PROPN
njb-38	233	4	s	s	PROPN
njb-38	233	5	,	,	PUNCT
njb-38	233	6	dresel	dresel	PROPN
njb-38	233	7	a	a	PRON
njb-38	233	8	,	,	PUNCT
njb-38	233	9	clos	clos	PROPN
njb-38	233	10	j	j	X
njb-38	233	11	:	:	PUNCT
njb-38	233	12	high	high	ADJ
njb-38	233	13	constitutive	constitutive	ADJ
njb-38	233	14	levels	level	NOUN
njb-38	233	15	of	of	ADP
njb-38	233	16	heat	heat	NOUN
njb-38	233	17	-	-	PUNCT
njb-38	233	18	shock	shock	NOUN
njb-38	233	19	proteins	protein	NOUN
njb-38	233	20	in	in	ADP
njb-38	233	21	humanpathogenic	humanpathogenic	ADJ
njb-38	233	22	parasites	parasite	NOUN
njb-38	233	23	of	of	ADP
njb-38	233	24	the	the	DET
njb-38	233	25	genus	genus	NOUN
njb-38	233	26	leishmania	leishmania	PROPN
njb-38	233	27	.	.	PUNCT
njb-38	234	1	biochemical	biochemical	ADJ
njb-38	234	2	journal	journal	PROPN
njb-38	234	3	,	,	PUNCT
njb-38	234	4	1995	1995	NUM
njb-38	234	5	,	,	PUNCT
njb-38	234	6	10:225	10:225	NUM
njb-38	234	7	-	-	SYM
njb-38	234	8	232	232	NUM
njb-38	234	9	.	.	NUM
njb-38	234	10	22	22	NUM
njb-38	234	11	.	.	PUNCT
njb-38	235	1	del	del	PROPN
njb-38	235	2	razo	razo	PROPN
njb-38	235	3	lm	lm	PROPN
njb-38	235	4	,	,	PUNCT
njb-38	235	5	quintanilla	quintanilla	PROPN
njb-38	235	6	-	-	PUNCT
njb-38	235	7	vega	vega	PROPN
njb-38	235	8	b	b	PROPN
njb-38	235	9	,	,	PUNCT
njb-38	235	10	brambilacolombres	brambilacolombres	PROPN
njb-38	235	11	e	e	PROPN
njb-38	235	12	,	,	PUNCT
njb-38	235	13	calderon	calderon	PROPN
njb-38	235	14	-	-	PUNCT
njb-38	235	15	aranda	aranda	PROPN
njb-38	235	16	es	es	PROPN
njb-38	235	17	,	,	PUNCT
njb-38	235	18	manno	manno	PROPN
njb-38	235	19	m	m	PROPN
njb-38	235	20	,	,	PUNCT
njb-38	235	21	albores	albore	VERB
njb-38	235	22	a	a	DET
njb-38	235	23	:	:	PUNCT
njb-38	235	24	stress	stress	NOUN
njb-38	235	25	proteins	protein	NOUN
njb-38	235	26	induced	induce	VERB
njb-38	235	27	by	by	ADP
njb-38	235	28	arsenic	arsenic	ADJ
njb-38	235	29	.	.	PUNCT
njb-38	236	1	toxicology	toxicology	NOUN
njb-38	236	2	and	and	CCONJ
njb-38	236	3	applied	apply	VERB
njb-38	236	4	pharmacology	pharmacology	NOUN
njb-38	236	5	,	,	PUNCT
njb-38	236	6	2001	2001	NUM
njb-38	236	7	177(2):132	177(2):132	NUM
njb-38	236	8	-	-	SYM
njb-38	236	9	148	148	NUM
njb-38	236	10	.	.	NUM
njb-38	236	11	23	23	NUM
njb-38	236	12	.	.	PUNCT
njb-38	237	1	lawrence	lawrence	PROPN
njb-38	237	2	f	f	PROPN
njb-38	237	3	,	,	PUNCT
njb-38	237	4	robert	robert	PROPN
njb-38	237	5	-	-	PUNCT
njb-38	237	6	gero	gero	PROPN
njb-38	237	7	m	m	PROPN
njb-38	237	8	:	:	PUNCT
njb-38	237	9	induction	induction	NOUN
njb-38	237	10	of	of	ADP
njb-38	237	11	heat	heat	NOUN
njb-38	237	12	shock	shock	NOUN
njb-38	237	13	and	and	CCONJ
njb-38	237	14	stress	stress	NOUN
njb-38	237	15	proteins	protein	NOUN
njb-38	237	16	in	in	ADP
njb-38	237	17	promastigotes	promastigote	NOUN
njb-38	237	18	of	of	ADP
njb-38	237	19	three	three	NUM
njb-38	237	20	leishmania	leishmania	PROPN
njb-38	237	21	species	specie	NOUN
njb-38	237	22	.	.	PUNCT
njb-38	238	1	proceedings	proceeding	NOUN
njb-38	238	2	of	of	ADP
njb-38	238	3	the	the	DET
njb-38	238	4	national	national	PROPN
njb-38	238	5	academy	academy	PROPN
njb-38	238	6	of	of	ADP
njb-38	238	7	sciences	sciences	PROPN
njb-38	238	8	,	,	PUNCT
njb-38	238	9	1985	1985	NUM
njb-38	238	10	,	,	PUNCT
njb-38	238	11	82(13):4414	82(13):4414	NUM
njb-38	238	12	-	-	SYM
njb-38	238	13	4417	4417	NUM
njb-38	238	14	.	.	PUNCT