id	sid	tid	token	lemma	pos
njb-39	1	1	nepal	nepal	ADJ
njb-39	1	2	journal	journal	NOUN
njb-39	1	3	of	of	ADP
njb-39	1	4	biotechnology	biotechnology	NOUN
njb-39	1	5	.	.	PUNCT
njb-39	2	1	d	d	X
njb-39	2	2	e	e	PROPN
njb-39	2	3	c	c	PROPN
njb-39	2	4	.	.	PUNCT
njb-39	3	1	2	2	NUM
njb-39	3	2	0	0	NUM
njb-39	3	3	1	1	NUM
njb-39	3	4	5	5	NUM
njb-39	3	5	vol	vol	NOUN
njb-39	3	6	.	.	PUNCT
njb-39	4	1	3	3	NUM
njb-39	4	2	,	,	PUNCT
njb-39	4	3	no	no	NOUN
njb-39	4	4	.	.	NOUN
njb-39	4	5	1:15	1:15	NUM
njb-39	4	6	-	-	SYM
njb-39	4	7	21	21	NUM
njb-39	4	8	issn	issn	PROPN
njb-39	4	9	2091	2091	NUM
njb-39	4	10	-	-	SYM
njb-39	4	11	1130	1130	NUM
njb-39	4	12	(	(	PUNCT
njb-39	4	13	print	print	NOUN
njb-39	4	14	)	)	PUNCT
njb-39	4	15	/issn	/issn	PUNCT
njb-39	5	1	2467	2467	NUM
njb-39	5	2	-	-	SYM
njb-39	5	3	9319	9319	NUM
njb-39	5	4	(	(	PUNCT
njb-39	5	5	online	online	ADJ
njb-39	5	6	)	)	PUNCT
njb-39	5	7	original	original	ADJ
njb-39	5	8	research	research	NOUN
njb-39	5	9	article	article	NOUN
njb-39	5	10	©	©	PROPN
njb-39	5	11	njb	njb	PROPN
njb-39	5	12	,	,	PUNCT
njb-39	5	13	biotechnology	biotechnology	NOUN
njb-39	5	14	society	society	NOUN
njb-39	5	15	of	of	ADP
njb-39	5	16	nepal	nepal	ADJ
njb-39	5	17	15	15	NUM
njb-39	5	18	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-39	5	19	effect	effect	NOUN
njb-39	5	20	of	of	ADP
njb-39	5	21	lyophilization	lyophilization	NOUN
njb-39	5	22	on	on	ADP
njb-39	5	23	infectivity	infectivity	NOUN
njb-39	5	24	and	and	CCONJ
njb-39	5	25	viral	viral	ADJ
njb-39	5	26	load	load	NOUN
njb-39	5	27	of	of	ADP
njb-39	5	28	adenovirus	adenovirus	PROPN
njb-39	5	29	bimlesh	bimlesh	PROPN
njb-39	5	30	kumar	kumar	PROPN
njb-39	5	31	jha1	jha1	PROPN
njb-39	5	32	*	*	PROPN
njb-39	5	33	,	,	PUNCT
njb-39	5	34	birendra	birendra	PROPN
njb-39	5	35	prasad	prasad	PROPN
njb-39	5	36	gupta	gupta	PROPN
njb-39	5	37	2	2	NUM
njb-39	5	38	,	,	PUNCT
njb-39	5	39	prashanna	prashanna	PROPN
njb-39	5	40	maharjan3	maharjan3	PROPN
njb-39	5	41	,	,	PUNCT
njb-39	5	42	somila	somila	PROPN
njb-39	5	43	kakshapati3	kakshapati3	PROPN
njb-39	5	44	,	,	PUNCT
njb-39	5	45	nabin	nabin	PROPN
njb-39	5	46	narayan	narayan	PROPN
njb-39	5	47	munankarmi,3	munankarmi,3	VERB
njb-39	5	48	1national	1national	NUM
njb-39	5	49	public	public	ADJ
njb-39	5	50	health	health	NOUN
njb-39	5	51	laboratory	laboratory	NOUN
njb-39	5	52	,	,	PUNCT
njb-39	5	53	teku	teku	NOUN
njb-39	5	54	,	,	PUNCT
njb-39	5	55	kathmandu	kathmandu	NOUN
njb-39	5	56	,	,	PUNCT
njb-39	5	57	nepal	nepal	ADJ
njb-39	5	58	2central	2central	NUM
njb-39	5	59	department	department	NOUN
njb-39	5	60	of	of	ADP
njb-39	5	61	biotechnology	biotechnology	NOUN
njb-39	5	62	,	,	PUNCT
njb-39	5	63	tribhuvan	tribhuvan	PROPN
njb-39	5	64	university	university	PROPN
njb-39	5	65	,	,	PUNCT
njb-39	5	66	kirtipur	kirtipur	PROPN
njb-39	5	67	,	,	PUNCT
njb-39	5	68	kathmandu	kathmandu	NOUN
njb-39	5	69	,	,	PUNCT
njb-39	5	70	nepal	nepal	ADJ
njb-39	5	71	3biotechnology	3biotechnology	NUM
njb-39	5	72	society	society	NOUN
njb-39	5	73	of	of	ADP
njb-39	5	74	nepal	nepal	PROPN
njb-39	5	75	(	(	PUNCT
njb-39	5	76	bsn	bsn	PROPN
njb-39	5	77	)	)	PUNCT
njb-39	5	78	,	,	PUNCT
njb-39	5	79	kathmandu	kathmandu	NOUN
njb-39	5	80	,	,	PUNCT
njb-39	5	81	nepal	nepal	ADJ
njb-39	5	82	abstract	abstract	ADJ
njb-39	5	83	freeze	freeze	VERB
njb-39	5	84	drying	dry	VERB
njb-39	5	85	(	(	PUNCT
njb-39	5	86	lyophilization	lyophilization	NOUN
njb-39	5	87	)	)	PUNCT
njb-39	5	88	performed	perform	VERB
njb-39	5	89	at	at	ADP
njb-39	5	90	temperature	temperature	NOUN
njb-39	5	91	and	and	CCONJ
njb-39	5	92	pressure	pressure	NOUN
njb-39	5	93	below	below	ADP
njb-39	5	94	the	the	DET
njb-39	5	95	triple	triple	ADJ
njb-39	5	96	point	point	NOUN
njb-39	5	97	is	be	AUX
njb-39	5	98	being	be	AUX
njb-39	5	99	practiced	practice	VERB
njb-39	5	100	for	for	ADP
njb-39	5	101	the	the	DET
njb-39	5	102	preservation	preservation	NOUN
njb-39	5	103	of	of	ADP
njb-39	5	104	virus	virus	NOUN
njb-39	5	105	stocks	stock	NOUN
njb-39	5	106	for	for	ADP
njb-39	5	107	longer	long	ADJ
njb-39	5	108	periods	period	NOUN
njb-39	5	109	.	.	PUNCT
njb-39	6	1	the	the	DET
njb-39	6	2	present	present	ADJ
njb-39	6	3	study	study	NOUN
njb-39	6	4	is	be	AUX
njb-39	6	5	aimed	aim	VERB
njb-39	6	6	to	to	PART
njb-39	6	7	lyophilize	lyophilize	VERB
njb-39	6	8	adenovirus	adenovirus	PROPN
njb-39	6	9	strain	strain	VERB
njb-39	6	10	to	to	PART
njb-39	6	11	study	study	VERB
njb-39	6	12	its	its	PRON
njb-39	6	13	effects	effect	NOUN
njb-39	6	14	on	on	ADP
njb-39	6	15	infectivity	infectivity	NOUN
njb-39	6	16	and	and	CCONJ
njb-39	6	17	viral	viral	ADJ
njb-39	6	18	load	load	NOUN
njb-39	6	19	.	.	PUNCT
njb-39	7	1	in	in	ADP
njb-39	7	2	-	-	PUNCT
njb-39	7	3	house	house	NOUN
njb-39	7	4	adenovirus	adenovirus	NOUN
njb-39	7	5	reference	reference	NOUN
njb-39	7	6	strain	strain	NOUN
njb-39	7	7	(	(	PUNCT
njb-39	7	8	stock	stock	NOUN
njb-39	7	9	virus	virus	NOUN
njb-39	7	10	)	)	PUNCT
njb-39	7	11	was	be	AUX
njb-39	7	12	propagated	propagate	VERB
njb-39	7	13	in	in	ADP
njb-39	7	14	hep-2	hep-2	PUNCT
njb-39	7	15	cell	cell	NOUN
njb-39	7	16	line	line	NOUN
njb-39	7	17	in	in	ADP
njb-39	7	18	25cm2	25cm2	ADJ
njb-39	7	19	cell	cell	NOUN
njb-39	7	20	culture	culture	NOUN
njb-39	7	21	flasks	flask	NOUN
njb-39	7	22	.	.	PUNCT
njb-39	8	1	in	in	ADP
njb-39	8	2	24	24	NUM
njb-39	8	3	-	-	PUNCT
njb-39	8	4	well	well	NOUN
njb-39	8	5	plates	plate	NOUN
njb-39	8	6	the	the	DET
njb-39	8	7	serial	serial	ADJ
njb-39	8	8	dilutions	dilution	NOUN
njb-39	8	9	of	of	ADP
njb-39	8	10	stock	stock	NOUN
njb-39	8	11	virus	virus	NOUN
njb-39	8	12	from	from	ADP
njb-39	8	13	10	10	NUM
njb-39	8	14	-	-	SYM
njb-39	8	15	1	1	NUM
njb-39	8	16	to	to	PART
njb-39	8	17	10	10	NUM
njb-39	8	18	-	-	SYM
njb-39	8	19	7	7	NUM
njb-39	8	20	(	(	PUNCT
njb-39	8	21	100µl	100µl	PROPN
njb-39	8	22	inoculum	inoculum	PROPN
njb-39	8	23	)	)	PUNCT
njb-39	8	24	was	be	AUX
njb-39	8	25	inoculated	inoculate	VERB
njb-39	8	26	in	in	ADP
njb-39	8	27	each	each	DET
njb-39	8	28	well	well	ADV
njb-39	8	29	with	with	ADP
njb-39	8	30	hep-2	hep-2	PUNCT
njb-39	8	31	cells	cell	NOUN
njb-39	8	32	for	for	ADP
njb-39	8	33	tcid50	tcid50	ADJ
njb-39	8	34	titer	titer	NOUN
njb-39	8	35	and	and	CCONJ
njb-39	8	36	viral	viral	ADJ
njb-39	8	37	dna	dna	NOUN
njb-39	8	38	was	be	AUX
njb-39	8	39	extracted	extract	VERB
njb-39	8	40	separately	separately	ADV
njb-39	8	41	to	to	PART
njb-39	8	42	determine	determine	VERB
njb-39	8	43	viral	viral	ADJ
njb-39	8	44	load	load	NOUN
njb-39	8	45	by	by	ADP
njb-39	8	46	taqman	taqman	NOUN
njb-39	8	47	real	real	ADJ
njb-39	8	48	time	time	NOUN
njb-39	8	49	pcr	pcr	PROPN
njb-39	8	50	.	.	PUNCT
njb-39	9	1	stock	stock	NOUN
njb-39	9	2	virus	virus	NOUN
njb-39	9	3	was	be	AUX
njb-39	9	4	lyophilized	lyophilize	VERB
njb-39	9	5	in	in	ADP
njb-39	9	6	3	3	NUM
njb-39	9	7	lots	lot	NOUN
njb-39	9	8	and	and	CCONJ
njb-39	9	9	stored	store	VERB
njb-39	9	10	at	at	ADP
njb-39	9	11	rt	rt	PROPN
njb-39	9	12	(	(	PUNCT
njb-39	9	13	25±2	25±2	NUM
njb-39	9	14	°	°	NOUN
njb-39	9	15	c	c	NOUN
njb-39	9	16	)	)	PUNCT
njb-39	9	17	and	and	CCONJ
njb-39	9	18	4	4	NUM
njb-39	9	19	°	°	NOUN
njb-39	9	20	c	c	NOUN
njb-39	9	21	separately	separately	ADV
njb-39	9	22	for	for	ADP
njb-39	9	23	1	1	NUM
njb-39	9	24	,	,	PUNCT
njb-39	9	25	4	4	NUM
njb-39	9	26	and	and	CCONJ
njb-39	9	27	6	6	NUM
njb-39	9	28	months	month	NOUN
njb-39	9	29	and	and	CCONJ
njb-39	9	30	subjected	subject	VERB
njb-39	9	31	to	to	ADP
njb-39	9	32	tcid50	tcid50	NOUN
njb-39	9	33	(	(	PUNCT
njb-39	9	34	for	for	ADP
njb-39	9	35	viral	viral	ADJ
njb-39	9	36	infectivity	infectivity	NOUN
njb-39	9	37	)	)	PUNCT
njb-39	9	38	and	and	CCONJ
njb-39	9	39	viral	viral	ADJ
njb-39	9	40	load	load	NOUN
njb-39	9	41	assay	assay	NOUN
njb-39	9	42	(	(	PUNCT
njb-39	9	43	for	for	ADP
njb-39	9	44	total	total	ADJ
njb-39	9	45	viral	viral	ADJ
njb-39	9	46	genome	genome	NOUN
njb-39	9	47	copies	copy	NOUN
njb-39	9	48	)	)	PUNCT
njb-39	9	49	.	.	PUNCT
njb-39	10	1	following	follow	VERB
njb-39	10	2	lyophilisation	lyophilisation	NOUN
njb-39	10	3	and	and	CCONJ
njb-39	10	4	storage	storage	NOUN
njb-39	10	5	of	of	ADP
njb-39	10	6	adenoviral	adenoviral	ADJ
njb-39	10	7	strains	strain	NOUN
njb-39	10	8	at	at	ADP
njb-39	10	9	rt	rt	PROPN
njb-39	10	10	and	and	CCONJ
njb-39	10	11	4	4	NUM
njb-39	10	12	°	°	NOUN
njb-39	10	13	c	c	NOUN
njb-39	10	14	separately	separately	ADV
njb-39	10	15	did	do	AUX
njb-39	10	16	not	not	PART
njb-39	10	17	affect	affect	VERB
njb-39	10	18	significantly	significantly	ADV
njb-39	10	19	on	on	ADP
njb-39	10	20	the	the	DET
njb-39	10	21	viral	viral	ADJ
njb-39	10	22	stability	stability	NOUN
njb-39	10	23	,	,	PUNCT
njb-39	10	24	infectivity	infectivity	NOUN
njb-39	10	25	as	as	ADV
njb-39	10	26	well	well	ADV
njb-39	10	27	as	as	ADP
njb-39	10	28	viral	viral	ADJ
njb-39	10	29	copy	copy	NOUN
njb-39	10	30	number	number	NOUN
njb-39	10	31	till	till	SCONJ
njb-39	10	32	4	4	NUM
njb-39	10	33	months	month	NOUN
njb-39	10	34	.	.	PUNCT
njb-39	11	1	however	however	ADV
njb-39	11	2	,	,	PUNCT
njb-39	11	3	storage	storage	NOUN
njb-39	11	4	at	at	ADP
njb-39	11	5	rt	rt	PROPN
njb-39	11	6	for	for	ADP
njb-39	11	7	6	6	NUM
njb-39	11	8	months	month	NOUN
njb-39	11	9	resulted	result	VERB
njb-39	11	10	in	in	ADP
njb-39	11	11	1	1	NUM
njb-39	11	12	log	log	NOUN
njb-39	11	13	reduction	reduction	NOUN
njb-39	11	14	in	in	ADP
njb-39	11	15	viral	viral	ADJ
njb-39	11	16	copy	copy	NOUN
njb-39	11	17	number	number	NOUN
njb-39	11	18	.	.	PUNCT
njb-39	12	1	thus	thus	ADV
njb-39	12	2	,	,	PUNCT
njb-39	12	3	storage	storage	NOUN
njb-39	12	4	of	of	ADP
njb-39	12	5	even	even	ADV
njb-39	12	6	lyophilized	lyophilize	VERB
njb-39	12	7	virus	virus	NOUN
njb-39	12	8	stock	stock	NOUN
njb-39	12	9	would	would	AUX
njb-39	12	10	necessitate	necessitate	VERB
njb-39	12	11	a	a	DET
njb-39	12	12	temperature	temperature	NOUN
njb-39	12	13	of	of	ADP
njb-39	12	14	at	at	ADV
njb-39	12	15	least	least	ADV
njb-39	12	16	4	4	NUM
njb-39	12	17	°	°	NOUN
njb-39	12	18	c	c	NOUN
njb-39	12	19	for	for	ADP
njb-39	12	20	prolonged	prolonged	ADJ
njb-39	12	21	periods	period	NOUN
njb-39	12	22	.	.	PUNCT
njb-39	13	1	the	the	DET
njb-39	13	2	present	present	ADJ
njb-39	13	3	study	study	NOUN
njb-39	13	4	could	could	AUX
njb-39	13	5	successfully	successfully	ADV
njb-39	13	6	lyophilize	lyophilize	VERB
njb-39	13	7	adenovirus	adenovirus	NOUN
njb-39	13	8	and	and	CCONJ
njb-39	13	9	retain	retain	VERB
njb-39	13	10	its	its	PRON
njb-39	13	11	infectivity	infectivity	NOUN
njb-39	13	12	over	over	ADP
njb-39	13	13	a	a	DET
njb-39	13	14	period	period	NOUN
njb-39	13	15	of	of	ADP
njb-39	13	16	6	6	NUM
njb-39	13	17	months	month	NOUN
njb-39	13	18	when	when	SCONJ
njb-39	13	19	stored	store	VERB
njb-39	13	20	at	at	ADP
njb-39	13	21	rt	rt	PROPN
njb-39	13	22	and	and	CCONJ
njb-39	13	23	4	4	NUM
njb-39	13	24	°	°	NOUN
njb-39	13	25	c	c	NOUN
njb-39	13	26	.	.	PUNCT
njb-39	14	1	no	no	DET
njb-39	14	2	significant	significant	ADJ
njb-39	14	3	difference	difference	NOUN
njb-39	14	4	in	in	ADP
njb-39	14	5	the	the	DET
njb-39	14	6	infectivity	infectivity	NOUN
njb-39	14	7	or	or	CCONJ
njb-39	14	8	tcid50	tcid50	NOUN
njb-39	14	9	titer	titer	NOUN
njb-39	14	10	was	be	AUX
njb-39	14	11	observed	observe	VERB
njb-39	14	12	in	in	ADP
njb-39	14	13	the	the	DET
njb-39	14	14	lyophilized	lyophilize	VERB
njb-39	14	15	virus	virus	NOUN
njb-39	14	16	as	as	SCONJ
njb-39	14	17	compared	compare	VERB
njb-39	14	18	to	to	ADP
njb-39	14	19	the	the	DET
njb-39	14	20	stock	stock	NOUN
njb-39	14	21	virus	virus	NOUN
njb-39	14	22	.	.	PUNCT
njb-39	15	1	however	however	ADV
njb-39	15	2	,	,	PUNCT
njb-39	15	3	the	the	DET
njb-39	15	4	viral	viral	ADJ
njb-39	15	5	load	load	NOUN
njb-39	15	6	was	be	AUX
njb-39	15	7	observed	observe	VERB
njb-39	15	8	to	to	PART
njb-39	15	9	increase	increase	VERB
njb-39	15	10	with	with	ADP
njb-39	15	11	lyophilization	lyophilization	NOUN
njb-39	15	12	of	of	ADP
njb-39	15	13	the	the	DET
njb-39	15	14	virus	virus	NOUN
njb-39	15	15	over	over	ADP
njb-39	15	16	6	6	NUM
njb-39	15	17	months	month	NOUN
njb-39	15	18	when	when	SCONJ
njb-39	15	19	stored	store	VERB
njb-39	15	20	at	at	ADP
njb-39	15	21	4	4	NUM
njb-39	15	22	°	°	NOUN
njb-39	15	23	c	c	NOUN
njb-39	15	24	which	which	PRON
njb-39	15	25	possibly	possibly	ADV
njb-39	15	26	is	be	AUX
njb-39	15	27	due	due	ADJ
njb-39	15	28	to	to	ADP
njb-39	15	29	the	the	DET
njb-39	15	30	concentration	concentration	NOUN
njb-39	15	31	of	of	ADP
njb-39	15	32	the	the	DET
njb-39	15	33	virus	virus	NOUN
njb-39	15	34	on	on	ADP
njb-39	15	35	freeze	freeze	NOUN
njb-39	15	36	-	-	PUNCT
njb-39	15	37	drying	drying	NOUN
njb-39	15	38	.	.	PUNCT
njb-39	16	1	keywords	keyword	NOUN
njb-39	16	2	:	:	PUNCT
njb-39	16	3	adenovirus	adenovirus	NOUN
njb-39	16	4	,	,	PUNCT
njb-39	16	5	lyophilisation	lyophilisation	NOUN
njb-39	16	6	,	,	PUNCT
njb-39	16	7	physical	physical	ADJ
njb-39	16	8	stability	stability	NOUN
njb-39	16	9	,	,	PUNCT
njb-39	16	10	formulation	formulation	NOUN
njb-39	16	11	*	*	SYM
njb-39	16	12	corresponding	correspond	VERB
njb-39	16	13	author	author	NOUN
njb-39	16	14	email	email	NOUN
njb-39	16	15	:	:	PUNCT
njb-39	16	16	jhabimlesh7@gmail.com	jhabimlesh7@gmail.com	X
njb-39	16	17	introduction	introduction	NOUN
njb-39	16	18	the	the	DET
njb-39	16	19	structure	structure	NOUN
njb-39	16	20	and	and	CCONJ
njb-39	16	21	function	function	NOUN
njb-39	16	22	of	of	ADP
njb-39	16	23	organisms	organism	NOUN
njb-39	16	24	change	change	VERB
njb-39	16	25	and	and	CCONJ
njb-39	16	26	get	get	AUX
njb-39	16	27	lost	lose	VERB
njb-39	16	28	with	with	ADP
njb-39	16	29	time	time	NOUN
njb-39	16	30	,	,	PUNCT
njb-39	16	31	as	as	ADP
njb-39	16	32	in	in	ADP
njb-39	16	33	laboratory	laboratory	NOUN
njb-39	16	34	cultures	culture	NOUN
njb-39	16	35	.	.	PUNCT
njb-39	17	1	attempts	attempt	NOUN
njb-39	17	2	to	to	PART
njb-39	17	3	stop	stop	VERB
njb-39	17	4	the	the	DET
njb-39	17	5	biological	biological	ADJ
njb-39	17	6	clock	clock	NOUN
njb-39	17	7	have	have	AUX
njb-39	17	8	been	be	AUX
njb-39	17	9	conjured	conjure	VERB
njb-39	17	10	by	by	ADP
njb-39	17	11	ancient	ancient	ADJ
njb-39	17	12	and	and	CCONJ
njb-39	17	13	modern	modern	ADJ
njb-39	17	14	minds	mind	NOUN
njb-39	17	15	;	;	PUNCT
njb-39	17	16	and	and	CCONJ
njb-39	17	17	the	the	DET
njb-39	17	18	heart	heart	NOUN
njb-39	17	19	of	of	ADP
njb-39	17	20	many	many	ADJ
njb-39	17	21	such	such	ADJ
njb-39	17	22	schemes	scheme	NOUN
njb-39	17	23	has	have	AUX
njb-39	17	24	been	be	AUX
njb-39	17	25	experiments	experiment	NOUN
njb-39	17	26	with	with	ADP
njb-39	17	27	temperature	temperature	NOUN
njb-39	17	28	and	and	CCONJ
njb-39	17	29	water	water	NOUN
njb-39	17	30	content	content	NOUN
njb-39	17	31	.	.	PUNCT
njb-39	18	1	whereas	whereas	SCONJ
njb-39	18	2	refrigeration	refrigeration	NOUN
njb-39	18	3	technology	technology	NOUN
njb-39	18	4	provides	provide	VERB
njb-39	18	5	a	a	DET
njb-39	18	6	means	means	NOUN
njb-39	18	7	of	of	ADP
njb-39	18	8	slowing	slow	VERB
njb-39	18	9	the	the	DET
njb-39	18	10	rate	rate	NOUN
njb-39	18	11	of	of	ADP
njb-39	18	12	deterioration	deterioration	NOUN
njb-39	18	13	of	of	ADP
njb-39	18	14	perishable	perishable	ADJ
njb-39	18	15	goods	good	NOUN
njb-39	18	16	,	,	PUNCT
njb-39	18	17	the	the	DET
njb-39	18	18	use	use	NOUN
njb-39	18	19	of	of	ADP
njb-39	18	20	much	much	ADJ
njb-39	18	21	lower	low	ADJ
njb-39	18	22	temperatures	temperature	NOUN
njb-39	18	23	has	have	AUX
njb-39	18	24	proved	prove	VERB
njb-39	18	25	a	a	DET
njb-39	18	26	means	means	NOUN
njb-39	18	27	of	of	ADP
njb-39	18	28	storing	store	VERB
njb-39	18	29	living	live	VERB
njb-39	18	30	organisms	organism	NOUN
njb-39	18	31	in	in	ADP
njb-39	18	32	a	a	DET
njb-39	18	33	state	state	NOUN
njb-39	18	34	of	of	ADP
njb-39	18	35	suspended	suspend	VERB
njb-39	18	36	animation	animation	NOUN
njb-39	18	37	for	for	ADP
njb-39	18	38	extended	extended	ADJ
njb-39	18	39	periods	period	NOUN
njb-39	18	40	.	.	PUNCT
njb-39	19	1	adenoviruses	adenovirus	NOUN
njb-39	19	2	(	(	PUNCT
njb-39	19	3	adv	adv	PROPN
njb-39	19	4	)	)	PUNCT
njb-39	19	5	,	,	PUNCT
njb-39	19	6	belonging	belong	VERB
njb-39	19	7	to	to	ADP
njb-39	19	8	family	family	NOUN
njb-39	19	9	adenoviridae	adenoviridae	PROPN
njb-39	19	10	,	,	PUNCT
njb-39	19	11	are	be	AUX
njb-39	19	12	double	double	ADV
njb-39	19	13	stranded	strand	VERB
njb-39	19	14	deoxyribonucleic	deoxyribonucleic	ADJ
njb-39	19	15	acid	acid	NOUN
njb-39	19	16	(	(	PUNCT
njb-39	19	17	dna	dna	PROPN
njb-39	19	18	)	)	PUNCT
njb-39	19	19	viruses	virus	NOUN
njb-39	19	20	that	that	PRON
njb-39	19	21	carry	carry	VERB
njb-39	19	22	dna	dna	PROPN
njb-39	19	23	insert	insert	NOUN
njb-39	19	24	of	of	ADP
njb-39	19	25	size	size	NOUN
njb-39	19	26	7	7	NUM
njb-39	19	27	kb	kb	PROPN
njb-39	19	28	.	.	PROPN
njb-39	19	29	52	52	NUM
njb-39	19	30	subtypes	subtype	NOUN
njb-39	19	31	of	of	ADP
njb-39	19	32	adenoviruses	adenovirus	NOUN
njb-39	19	33	are	be	AUX
njb-39	19	34	known	know	VERB
njb-39	19	35	to	to	PART
njb-39	19	36	infect	infect	VERB
njb-39	19	37	humans	human	NOUN
njb-39	19	38	[	[	X
njb-39	19	39	1	1	NUM
njb-39	19	40	]	]	X
njb-39	19	41	.	.	PUNCT
njb-39	20	1	adenoviruses	adenovirus	NOUN
njb-39	20	2	are	be	AUX
njb-39	20	3	frequent	frequent	ADJ
njb-39	20	4	cause	cause	NOUN
njb-39	20	5	of	of	ADP
njb-39	20	6	human	human	ADJ
njb-39	20	7	mucosal	mucosal	NOUN
njb-39	20	8	surface	surface	NOUN
njb-39	20	9	,	,	PUNCT
njb-39	20	10	infections	infection	NOUN
njb-39	20	11	particularly	particularly	ADV
njb-39	20	12	in	in	ADP
njb-39	20	13	pediatric	pediatric	ADJ
njb-39	20	14	population	population	NOUN
njb-39	20	15	and	and	CCONJ
njb-39	20	16	can	can	AUX
njb-39	20	17	be	be	AUX
njb-39	20	18	responsible	responsible	ADJ
njb-39	20	19	for	for	ADP
njb-39	20	20	ocular	ocular	ADJ
njb-39	20	21	and	and	CCONJ
njb-39	20	22	gastrointestinal	gastrointestinal	ADJ
njb-39	20	23	illnesses	illness	NOUN
njb-39	20	24	in	in	ADP
njb-39	20	25	humans	human	NOUN
njb-39	20	26	.	.	PUNCT
njb-39	21	1	to	to	PART
njb-39	21	2	preserve	preserve	VERB
njb-39	21	3	maximum	maximum	ADJ
njb-39	21	4	infectivity	infectivity	NOUN
njb-39	21	5	for	for	ADP
njb-39	21	6	long	long	ADJ
njb-39	21	7	periods	period	NOUN
njb-39	21	8	,	,	PUNCT
njb-39	21	9	cell	cell	NOUN
njb-39	21	10	culturegrown	culturegrown	NOUN
njb-39	21	11	adenovirus	adenovirus	NOUN
njb-39	21	12	must	must	AUX
njb-39	21	13	be	be	AUX
njb-39	21	14	stored	store	VERB
njb-39	21	15	frozen	frozen	ADJ
njb-39	21	16	at	at	ADP
njb-39	21	17	very	very	ADV
njb-39	21	18	low	low	ADJ
njb-39	21	19	temperatures	temperature	NOUN
njb-39	21	20	[	[	PUNCT
njb-39	21	21	2	2	NUM
njb-39	21	22	]	]	X
njb-39	21	23	.	.	PUNCT
njb-39	22	1	however	however	ADV
njb-39	22	2	,	,	PUNCT
njb-39	22	3	this	this	PRON
njb-39	22	4	makes	make	VERB
njb-39	22	5	its	its	PRON
njb-39	22	6	handling	handling	NOUN
njb-39	22	7	difficult	difficult	ADJ
njb-39	22	8	.	.	PUNCT
njb-39	23	1	the	the	DET
njb-39	23	2	removal	removal	NOUN
njb-39	23	3	of	of	ADP
njb-39	23	4	water	water	NOUN
njb-39	23	5	from	from	ADP
njb-39	23	6	viable	viable	ADJ
njb-39	23	7	biological	biological	ADJ
njb-39	23	8	material	material	NOUN
njb-39	23	9	in	in	ADP
njb-39	23	10	the	the	DET
njb-39	23	11	frozen	frozen	ADJ
njb-39	23	12	state	state	NOUN
njb-39	23	13	(	(	PUNCT
njb-39	23	14	freeze	freeze	NOUN
njb-39	23	15	-	-	PUNCT
njb-39	23	16	drying	drying	NOUN
njb-39	23	17	)	)	PUNCT
njb-39	23	18	provides	provide	VERB
njb-39	23	19	another	another	DET
njb-39	23	20	means	mean	NOUN
njb-39	23	21	of	of	ADP
njb-39	23	22	arresting	arrest	VERB
njb-39	23	23	the	the	DET
njb-39	23	24	biological	biological	ADJ
njb-39	23	25	clock	clock	NOUN
njb-39	23	26	by	by	ADP
njb-39	23	27	withholding	withhold	VERB
njb-39	23	28	water	water	NOUN
njb-39	23	29	,	,	PUNCT
njb-39	23	30	and	and	CCONJ
njb-39	23	31	commencing	commence	VERB
njb-39	23	32	again	again	ADV
njb-39	23	33	by	by	ADP
njb-39	23	34	its	its	PRON
njb-39	23	35	addition	addition	NOUN
njb-39	23	36	.	.	PUNCT
njb-39	24	1	lyophilization	lyophilization	NOUN
njb-39	24	2	or	or	CCONJ
njb-39	24	3	freeze	freeze	NOUN
njb-39	24	4	-	-	PUNCT
njb-39	24	5	drying	drying	NOUN
njb-39	24	6	is	be	AUX
njb-39	24	7	a	a	DET
njb-39	24	8	controllable	controllable	ADJ
njb-39	24	9	method	method	NOUN
njb-39	24	10	of	of	ADP
njb-39	24	11	dehydrating	dehydrate	VERB
njb-39	24	12	labile	labile	ADJ
njb-39	24	13	products	product	NOUN
njb-39	24	14	by	by	ADP
njb-39	24	15	vacuum	vacuum	NOUN
njb-39	24	16	desiccation	desiccation	NOUN
njb-39	24	17	[	[	X
njb-39	24	18	3	3	NUM
njb-39	24	19	]	]	X
njb-39	24	20	.	.	PUNCT
njb-39	25	1	it	it	PRON
njb-39	25	2	is	be	AUX
njb-39	25	3	considered	consider	VERB
njb-39	25	4	advantageous	advantageous	ADJ
njb-39	25	5	to	to	PART
njb-39	25	6	freeze	freeze	VERB
njb-39	25	7	dry	dry	ADJ
njb-39	25	8	viruses	virus	NOUN
njb-39	25	9	and	and	CCONJ
njb-39	25	10	vaccines	vaccine	NOUN
njb-39	25	11	wherever	wherever	SCONJ
njb-39	25	12	possible	possible	ADJ
njb-39	25	13	in	in	ADP
njb-39	25	14	order	order	NOUN
njb-39	25	15	to	to	PART
njb-39	25	16	reduce	reduce	VERB
njb-39	25	17	their	their	PRON
njb-39	25	18	volume	volume	NOUN
njb-39	25	19	for	for	ADP
njb-39	25	20	storage	storage	NOUN
njb-39	25	21	in	in	ADP
njb-39	25	22	cold	cold	ADJ
njb-39	25	23	,	,	PUNCT
njb-39	25	24	to	to	PART
njb-39	25	25	enable	enable	VERB
njb-39	25	26	easy	easy	ADJ
njb-39	25	27	handling	handling	NOUN
njb-39	25	28	and	and	CCONJ
njb-39	25	29	transport	transport	NOUN
njb-39	25	30	,	,	PUNCT
njb-39	25	31	and	and	CCONJ
njb-39	25	32	to	to	PART
njb-39	25	33	enhance	enhance	VERB
njb-39	25	34	their	their	PRON
njb-39	25	35	keeping	keep	VERB
njb-39	25	36	quality	quality	NOUN
njb-39	25	37	[	[	PUNCT
njb-39	25	38	4	4	NUM
njb-39	25	39	]	]	X
njb-39	25	40	.	.	PUNCT
njb-39	25	41	materials	material	NOUN
njb-39	25	42	and	and	CCONJ
njb-39	25	43	methods	method	NOUN
njb-39	25	44	cell	cell	NOUN
njb-39	25	45	line	line	NOUN
njb-39	25	46	and	and	CCONJ
njb-39	25	47	virus	virus	NOUN
njb-39	25	48	stock	stock	NOUN
njb-39	25	49	preparation	preparation	NOUN
njb-39	25	50	a	a	DET
njb-39	25	51	confluent	confluent	ADJ
njb-39	25	52	monolayer	monolayer	NOUN
njb-39	25	53	(	(	PUNCT
njb-39	25	54	90	90	NUM
njb-39	25	55	%	%	NOUN
njb-39	25	56	)	)	PUNCT
njb-39	25	57	of	of	ADP
njb-39	25	58	the	the	DET
njb-39	25	59	hep-2	hep-2	PUNCT
njb-39	25	60	cell	cell	NOUN
njb-39	25	61	lines	line	NOUN
njb-39	25	62	were	be	AUX
njb-39	25	63	grown	grow	VERB
njb-39	25	64	in	in	ADP
njb-39	25	65	25	25	NUM
njb-39	25	66	mm²	mm²	NOUN
njb-39	25	67	presterile	presterile	PROPN
njb-39	25	68	disposable	disposable	ADJ
njb-39	25	69	nunc	nunc	PROPN
njb-39	25	70	tissue	tissue	NOUN
njb-39	25	71	culture	culture	NOUN
njb-39	25	72	flask	flask	NOUN
njb-39	25	73	using	use	VERB
njb-39	25	74	(	(	PUNCT
njb-39	25	75	full	full	ADJ
njb-39	25	76	form?)mem	form?)mem	PROPN
njb-39	25	77	with	with	ADP
njb-39	25	78	antibiotics	antibiotic	NOUN
njb-39	25	79	(	(	PUNCT
njb-39	25	80	penicillin	penicillin	PROPN
njb-39	25	81	,	,	PUNCT
njb-39	25	82	streptomycin	streptomycin	PROPN
njb-39	25	83	)	)	PUNCT
njb-39	25	84	and	and	CCONJ
njb-39	25	85	10	10	NUM
njb-39	25	86	%	%	NOUN
njb-39	25	87	fetal	fetal	ADJ
njb-39	25	88	calf	calf	NOUN
njb-39	25	89	serum	serum	NOUN
njb-39	25	90	(	(	PUNCT
njb-39	25	91	fcs	fc	NOUN
njb-39	25	92	)	)	PUNCT
njb-39	25	93	.	.	PUNCT
njb-39	26	1	adenovirus	adenovirus	NOUN
njb-39	26	2	reference	reference	NOUN
njb-39	26	3	strain	strain	NOUN
njb-39	26	4	isolated	isolate	VERB
njb-39	26	5	from	from	ADP
njb-39	26	6	department	department	NOUN
njb-39	26	7	of	of	ADP
njb-39	26	8	virology	virology	NOUN
njb-39	26	9	was	be	AUX
njb-39	26	10	propagated	propagate	VERB
njb-39	26	11	for	for	ADP
njb-39	26	12	virus	virus	NOUN
njb-39	26	13	stock	stock	NOUN
njb-39	26	14	preparation	preparation	NOUN
njb-39	26	15	in	in	ADP
njb-39	26	16	hep2cell	hep2cell	PROPN
njb-39	26	17	line	line	NOUN
njb-39	26	18	and	and	CCONJ
njb-39	26	19	used	use	VERB
njb-39	26	20	for	for	ADP
njb-39	26	21	tcid50	tcid50	NOUN
njb-39	26	22	assay	assay	NOUN
njb-39	26	23	and	and	CCONJ
njb-39	26	24	viral	viral	ADJ
njb-39	26	25	load	load	NOUN
njb-39	26	26	.	.	PUNCT
njb-39	27	1	virus	virus	NOUN
njb-39	27	2	titration	titration	NOUN
njb-39	27	3	by	by	ADP
njb-39	27	4	tissue	tissue	NOUN
njb-39	27	5	culture	culture	NOUN
njb-39	27	6	infectious	infectious	ADJ
njb-39	27	7	dose	dose	NOUN
njb-39	27	8	50	50	NUM
njb-39	27	9	(	(	PUNCT
njb-39	27	10	tcid50	tcid50	NOUN
njb-39	27	11	)	)	PUNCT
njb-39	27	12	assay	assay	PROPN
njb-39	27	13	hep2	hep2	PROPN
njb-39	27	14	cell	cell	NOUN
njb-39	27	15	monolayer	monolayer	NOUN
njb-39	27	16	was	be	AUX
njb-39	27	17	prepared	prepared	ADJ
njb-39	27	18	and	and	CCONJ
njb-39	27	19	virus	virus	NOUN
njb-39	27	20	infection	infection	NOUN
njb-39	27	21	was	be	AUX
njb-39	27	22	performed	perform	VERB
njb-39	27	23	at	at	ADP
njb-39	27	24	different	different	ADJ
njb-39	27	25	dilutions	dilution	NOUN
njb-39	27	26	in	in	ADP
njb-39	27	27	10ml	10ml	PROPN
njb-39	27	28	sterile	sterile	ADJ
njb-39	27	29	nepal	nepal	ADJ
njb-39	27	30	journal	journal	NOUN
njb-39	27	31	of	of	ADP
njb-39	27	32	biotechnology	biotechnology	NOUN
njb-39	27	33	.	.	PUNCT
njb-39	28	1	d	d	X
njb-39	28	2	e	e	PROPN
njb-39	28	3	c	c	PROPN
njb-39	28	4	.	.	PUNCT
njb-39	29	1	2	2	NUM
njb-39	29	2	0	0	NUM
njb-39	29	3	1	1	NUM
njb-39	29	4	5	5	NUM
njb-39	29	5	vol	vol	NOUN
njb-39	29	6	.	.	PUNCT
njb-39	30	1	3	3	NUM
njb-39	30	2	,	,	PUNCT
njb-39	30	3	no	no	NOUN
njb-39	30	4	.	.	NOUN
njb-39	30	5	1:15	1:15	NUM
njb-39	30	6	-	-	SYM
njb-39	30	7	21	21	NUM
njb-39	30	8	jha	jha	NOUN
njb-39	30	9	et	et	PROPN
njb-39	30	10	al	al	PROPN
njb-39	30	11	.	.	PUNCT
njb-39	31	1	©	©	PROPN
njb-39	31	2	njb	njb	PROPN
njb-39	31	3	,	,	PUNCT
njb-39	31	4	biotechnology	biotechnology	NOUN
njb-39	31	5	society	society	NOUN
njb-39	31	6	of	of	ADP
njb-39	31	7	nepal	nepal	ADJ
njb-39	31	8	16	16	NUM
njb-39	31	9	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-39	31	10	tissue	tissue	NOUN
njb-39	31	11	culture	culture	NOUN
njb-39	31	12	tubes	tube	NOUN
njb-39	31	13	.	.	PUNCT
njb-39	32	1	cell	cell	NOUN
njb-39	32	2	monolayer	monolayer	NOUN
njb-39	32	3	was	be	AUX
njb-39	32	4	observed	observe	VERB
njb-39	32	5	daily	daily	ADV
njb-39	32	6	till	till	SCONJ
njb-39	32	7	the	the	DET
njb-39	32	8	cytopathic	cytopathic	ADJ
njb-39	32	9	effect	effect	NOUN
njb-39	32	10	(	(	PUNCT
njb-39	32	11	cpe	cpe	X
njb-39	32	12	)	)	PUNCT
njb-39	32	13	appeared	appear	VERB
njb-39	32	14	,	,	PUNCT
njb-39	32	15	along	along	ADP
njb-39	32	16	with	with	ADP
njb-39	32	17	the	the	DET
njb-39	32	18	control	control	NOUN
njb-39	32	19	wells	well	NOUN
njb-39	32	20	(	(	PUNCT
njb-39	32	21	mem	mem	PROPN
njb-39	32	22	inoculated	inoculate	VERB
njb-39	32	23	)	)	PUNCT
njb-39	32	24	kept	keep	VERB
njb-39	32	25	under	under	ADP
njb-39	32	26	the	the	DET
njb-39	32	27	same	same	ADJ
njb-39	32	28	condition	condition	NOUN
njb-39	32	29	.	.	PUNCT
njb-39	33	1	tcid50	tcid50	PROPN
njb-39	33	2	titer	titer	NOUN
njb-39	33	3	was	be	AUX
njb-39	33	4	be	be	AUX
njb-39	33	5	calculated	calculate	VERB
njb-39	33	6	by	by	ADP
njb-39	33	7	using	use	VERB
njb-39	33	8	reed	reed	NOUN
njb-39	33	9	and	and	CCONJ
njb-39	33	10	muench	muench	NOUN
njb-39	34	1	[	[	X
njb-39	34	2	5	5	NUM
njb-39	34	3	]	]	PUNCT
njb-39	34	4	.	.	PUNCT
njb-39	35	1	extraction	extraction	NOUN
njb-39	35	2	of	of	ADP
njb-39	35	3	viral	viral	ADJ
njb-39	35	4	dna	dna	PROPN
njb-39	35	5	viral	viral	ADJ
njb-39	35	6	dna	dna	NOUN
njb-39	35	7	was	be	AUX
njb-39	35	8	be	be	AUX
njb-39	35	9	extracted	extract	VERB
njb-39	35	10	from	from	ADP
njb-39	35	11	200µl	200µl	PROPN
njb-39	35	12	of	of	ADP
njb-39	35	13	tissue	tissue	NOUN
njb-39	35	14	culture	culture	NOUN
njb-39	35	15	lysate	lysate	NOUN
njb-39	35	16	using	use	VERB
njb-39	35	17	rneasy	rneasy	NOUN
njb-39	35	18	minikit	minikit	NOUN
njb-39	35	19	(	(	PUNCT
njb-39	35	20	qiagen	qiagen	PROPN
njb-39	35	21	,	,	PUNCT
njb-39	35	22	gmbh	gmbh	PROPN
njb-39	35	23	,	,	PUNCT
njb-39	35	24	hilden	hilden	PROPN
njb-39	35	25	,	,	PUNCT
njb-39	35	26	germany	germany	PROPN
njb-39	35	27	)	)	PUNCT
njb-39	35	28	according	accord	VERB
njb-39	35	29	to	to	ADP
njb-39	35	30	the	the	DET
njb-39	35	31	manufacturer	manufacturer	NOUN
njb-39	35	32	’s	’s	PART
njb-39	35	33	instructions	instruction	NOUN
njb-39	35	34	.	.	PUNCT
njb-39	36	1	in	in	ADP
njb-39	36	2	brief	brief	ADJ
njb-39	36	3	,	,	PUNCT
njb-39	36	4	200µl	200µl	ADJ
njb-39	36	5	of	of	ADP
njb-39	36	6	avl	avl	NOUN
njb-39	36	7	buffer	buffer	NOUN
njb-39	36	8	containing	contain	VERB
njb-39	36	9	20µl	20µl	NOUN
njb-39	36	10	of	of	ADP
njb-39	36	11	carrier	carrier	NOUN
njb-39	36	12	rna	rna	PROPN
njb-39	36	13	were	be	AUX
njb-39	36	14	dispensed	dispense	VERB
njb-39	36	15	in	in	ADP
njb-39	36	16	1.5	1.5	NUM
njb-39	36	17	ml	ml	NOUN
njb-39	36	18	of	of	ADP
njb-39	36	19	micro	micro	NOUN
njb-39	36	20	centrifuge	centrifuge	NOUN
njb-39	36	21	tube	tube	NOUN
njb-39	36	22	.	.	PUNCT
njb-39	37	1	200µl	200µl	PROPN
njb-39	37	2	of	of	ADP
njb-39	37	3	tissue	tissue	NOUN
njb-39	37	4	culture	culture	NOUN
njb-39	37	5	fluid	fluid	NOUN
njb-39	37	6	was	be	AUX
njb-39	37	7	added	add	VERB
njb-39	37	8	to	to	ADP
njb-39	37	9	the	the	DET
njb-39	37	10	buffer	buffer	NOUN
njb-39	37	11	avl	avl	NOUN
njb-39	37	12	containing	contain	VERB
njb-39	37	13	proteinase	proteinase	NOUN
njb-39	37	14	k	k	PROPN
njb-39	37	15	into	into	ADP
njb-39	37	16	micro	micro	NOUN
njb-39	37	17	centrifuge	centrifuge	NOUN
njb-39	37	18	tube	tube	NOUN
njb-39	37	19	and	and	CCONJ
njb-39	37	20	is	be	AUX
njb-39	37	21	mixed	mix	VERB
njb-39	37	22	by	by	ADP
njb-39	37	23	vortexing	vortexe	VERB
njb-39	37	24	for	for	ADP
njb-39	37	25	15	15	NUM
njb-39	37	26	sec	sec	PROPN
njb-39	37	27	.	.	PUNCT
njb-39	38	1	the	the	DET
njb-39	38	2	suspension	suspension	NOUN
njb-39	38	3	was	be	AUX
njb-39	38	4	incubated	incubate	VERB
njb-39	38	5	in	in	ADP
njb-39	38	6	the	the	DET
njb-39	38	7	dry	dry	ADJ
njb-39	38	8	temperature	temperature	NOUN
njb-39	38	9	bath	bath	NOUN
njb-39	38	10	(	(	PUNCT
njb-39	38	11	56	56	NUM
njb-39	38	12	°	°	NUM
njb-39	38	13	c	c	NOUN
njb-39	38	14	)	)	PUNCT
njb-39	38	15	for	for	ADP
njb-39	38	16	10	10	NUM
njb-39	38	17	minutes	minute	NOUN
njb-39	38	18	followed	follow	VERB
njb-39	38	19	by	by	ADP
njb-39	38	20	the	the	DET
njb-39	38	21	centrifugation	centrifugation	NOUN
njb-39	38	22	.	.	PUNCT
njb-39	39	1	after	after	ADP
njb-39	39	2	adding	add	VERB
njb-39	39	3	200µl	200µl	ADJ
njb-39	39	4	of	of	ADP
njb-39	39	5	absolute	absolute	ADJ
njb-39	39	6	ethanol	ethanol	NOUN
njb-39	39	7	,	,	PUNCT
njb-39	39	8	the	the	DET
njb-39	39	9	sample	sample	NOUN
njb-39	39	10	was	be	AUX
njb-39	39	11	vortexed	vortexe	VERB
njb-39	39	12	for	for	ADP
njb-39	39	13	15sec	15sec	PROPN
njb-39	39	14	and	and	CCONJ
njb-39	39	15	centrifuged	centrifuge	VERB
njb-39	39	16	briefly	briefly	ADV
njb-39	39	17	.	.	PUNCT
njb-39	40	1	carefully	carefully	ADV
njb-39	40	2	620µl	620µl	NOUN
njb-39	40	3	of	of	ADP
njb-39	40	4	the	the	DET
njb-39	40	5	solution	solution	NOUN
njb-39	40	6	was	be	AUX
njb-39	40	7	be	be	AUX
njb-39	40	8	applied	apply	VERB
njb-39	40	9	to	to	ADP
njb-39	40	10	the	the	DET
njb-39	40	11	qiaamp	qiaamp	NOUN
njb-39	40	12	mini	mini	ADJ
njb-39	40	13	column	column	NOUN
njb-39	40	14	with	with	ADP
njb-39	40	15	2ml	2ml	NOUN
njb-39	40	16	of	of	ADP
njb-39	40	17	collecting	collect	VERB
njb-39	40	18	tube	tube	NOUN
njb-39	40	19	without	without	ADP
njb-39	40	20	wetting	wet	VERB
njb-39	40	21	the	the	DET
njb-39	40	22	rim	rim	NOUN
njb-39	40	23	.	.	PUNCT
njb-39	41	1	the	the	DET
njb-39	41	2	cap	cap	NOUN
njb-39	41	3	is	be	AUX
njb-39	41	4	closed	close	VERB
njb-39	41	5	and	and	CCONJ
njb-39	41	6	centrifuged	centrifuge	VERB
njb-39	41	7	at	at	ADP
njb-39	41	8	6000	6000	NUM
njb-39	41	9	g	g	NOUN
njb-39	41	10	(	(	PUNCT
njb-39	41	11	8000rpm	8000rpm	NUM
njb-39	41	12	)	)	PUNCT
njb-39	41	13	for	for	ADP
njb-39	41	14	1	1	NUM
njb-39	41	15	min	min	NOUN
njb-39	41	16	.	.	PUNCT
njb-39	42	1	the	the	DET
njb-39	42	2	qiaamp	qiaamp	PROPN
njb-39	42	3	mini	mini	ADJ
njb-39	42	4	column	column	NOUN
njb-39	42	5	is	be	AUX
njb-39	42	6	placed	place	VERB
njb-39	42	7	into	into	ADP
njb-39	42	8	the	the	DET
njb-39	42	9	2ml	2ml	NOUN
njb-39	42	10	of	of	ADP
njb-39	42	11	clean	clean	ADJ
njb-39	42	12	collecting	collecting	NOUN
njb-39	42	13	tube	tube	NOUN
njb-39	42	14	and	and	CCONJ
njb-39	42	15	the	the	DET
njb-39	42	16	tube	tube	NOUN
njb-39	42	17	containing	contain	VERB
njb-39	42	18	filtrate	filtrate	NOUN
njb-39	42	19	were	be	AUX
njb-39	42	20	discarded	discard	VERB
njb-39	42	21	.	.	PUNCT
njb-39	43	1	the	the	DET
njb-39	43	2	qiaamp	qiaamp	PROPN
njb-39	43	3	mini	mini	ADJ
njb-39	43	4	column	column	NOUN
njb-39	43	5	were	be	AUX
njb-39	43	6	opened	open	VERB
njb-39	43	7	carefully	carefully	ADV
njb-39	43	8	and	and	CCONJ
njb-39	43	9	500µl	500µl	PROPN
njb-39	43	10	of	of	ADP
njb-39	43	11	aw1	aw1	PROPN
njb-39	43	12	buffer	buffer	PROPN
njb-39	43	13	were	be	AUX
njb-39	43	14	added	add	VERB
njb-39	43	15	to	to	ADP
njb-39	43	16	the	the	DET
njb-39	43	17	qiaamp	qiaamp	NOUN
njb-39	43	18	mini	mini	ADJ
njb-39	43	19	column	column	NOUN
njb-39	43	20	and	and	CCONJ
njb-39	43	21	centrifuged	centrifuge	VERB
njb-39	43	22	at	at	ADP
njb-39	43	23	10000	10000	NUM
njb-39	43	24	g	g	NOUN
njb-39	43	25	for	for	ADP
njb-39	43	26	1	1	NUM
njb-39	43	27	minute	minute	NOUN
njb-39	43	28	.	.	PUNCT
njb-39	44	1	followed	follow	VERB
njb-39	44	2	by	by	ADP
njb-39	44	3	the	the	DET
njb-39	44	4	replacement	replacement	NOUN
njb-39	44	5	of	of	ADP
njb-39	44	6	new	new	ADJ
njb-39	44	7	2ml	2ml	NOUN
njb-39	44	8	of	of	ADP
njb-39	44	9	collection	collection	NOUN
njb-39	44	10	tube	tube	NOUN
njb-39	44	11	and	and	CCONJ
njb-39	44	12	the	the	DET
njb-39	44	13	tube	tube	NOUN
njb-39	44	14	containing	contain	VERB
njb-39	44	15	filtrate	filtrate	NOUN
njb-39	44	16	were	be	AUX
njb-39	44	17	discarded	discard	VERB
njb-39	44	18	.	.	PUNCT
njb-39	45	1	500µl	500µl	PROPN
njb-39	45	2	of	of	ADP
njb-39	45	3	the	the	DET
njb-39	45	4	aw2	aw2	ADJ
njb-39	45	5	buffer	buffer	NOUN
njb-39	45	6	was	be	AUX
njb-39	45	7	added	add	VERB
njb-39	45	8	to	to	ADP
njb-39	45	9	the	the	DET
njb-39	45	10	column	column	NOUN
njb-39	45	11	and	and	CCONJ
njb-39	45	12	centrifuged	centrifuge	VERB
njb-39	45	13	at	at	ADP
njb-39	45	14	20000	20000	NUM
njb-39	45	15	g	g	NOUN
njb-39	45	16	for	for	ADP
njb-39	45	17	3	3	NUM
njb-39	45	18	minutes	minute	NOUN
njb-39	45	19	.	.	PUNCT
njb-39	46	1	followed	follow	VERB
njb-39	46	2	by	by	ADP
njb-39	46	3	the	the	DET
njb-39	46	4	replacement	replacement	NOUN
njb-39	46	5	of	of	ADP
njb-39	46	6	new	new	ADJ
njb-39	46	7	2ml	2ml	NOUN
njb-39	46	8	of	of	ADP
njb-39	46	9	collection	collection	NOUN
njb-39	46	10	tube	tube	NOUN
njb-39	46	11	and	and	CCONJ
njb-39	46	12	the	the	DET
njb-39	46	13	tube	tube	NOUN
njb-39	46	14	containing	contain	VERB
njb-39	46	15	filtrate	filtrate	NOUN
njb-39	46	16	were	be	AUX
njb-39	46	17	discarded	discard	VERB
njb-39	46	18	and	and	CCONJ
njb-39	46	19	centrifuged	centrifuge	VERB
njb-39	46	20	at	at	ADP
njb-39	46	21	full	full	ADJ
njb-39	46	22	speed	speed	NOUN
njb-39	46	23	for	for	ADP
njb-39	46	24	1	1	NUM
njb-39	46	25	minute	minute	NOUN
njb-39	46	26	.	.	PUNCT
njb-39	47	1	finally	finally	ADV
njb-39	47	2	the	the	DET
njb-39	47	3	qia	qia	PROPN
njb-39	47	4	amp	amp	PROPN
njb-39	47	5	mini	mini	PROPN
njb-39	47	6	column	column	NOUN
njb-39	47	7	was	be	AUX
njb-39	47	8	placed	place	VERB
njb-39	47	9	in	in	ADP
njb-39	47	10	a	a	DET
njb-39	47	11	sterile	sterile	ADJ
njb-39	47	12	,	,	PUNCT
njb-39	47	13	dnaase	dnaase	PROPN
njb-39	47	14	,	,	PUNCT
njb-39	47	15	rnaase	rnaase	PROPN
njb-39	47	16	,	,	PUNCT
njb-39	47	17	free	free	ADJ
njb-39	47	18	1.5ml	1.5ml	NUM
njb-39	47	19	of	of	ADP
njb-39	47	20	the	the	DET
njb-39	47	21	micro	micro	ADJ
njb-39	47	22	centrifuge	centrifuge	NOUN
njb-39	47	23	tube	tube	NOUN
njb-39	47	24	.	.	PUNCT
njb-39	48	1	the	the	DET
njb-39	48	2	old	old	ADJ
njb-39	48	3	collecting	collecting	NOUN
njb-39	48	4	tube	tube	NOUN
njb-39	48	5	containing	contain	VERB
njb-39	48	6	the	the	DET
njb-39	48	7	filtrate	filtrate	NOUN
njb-39	48	8	was	be	AUX
njb-39	48	9	discarded	discard	VERB
njb-39	48	10	.	.	PUNCT
njb-39	49	1	50µl	50µl	PROPN
njb-39	49	2	of	of	ADP
njb-39	49	3	ave	ave	PROPN
njb-39	49	4	buffer	buffer	NOUN
njb-39	49	5	was	be	AUX
njb-39	49	6	added	add	VERB
njb-39	49	7	to	to	ADP
njb-39	49	8	the	the	DET
njb-39	49	9	qiaamp	qiaamp	NOUN
njb-39	49	10	mini	mini	ADJ
njb-39	49	11	column	column	NOUN
njb-39	49	12	and	and	CCONJ
njb-39	49	13	were	be	AUX
njb-39	49	14	equilibrated	equilibrate	VERB
njb-39	49	15	to	to	ADP
njb-39	49	16	room	room	NOUN
njb-39	49	17	temperature	temperature	NOUN
njb-39	49	18	for	for	ADP
njb-39	49	19	1	1	NUM
njb-39	49	20	min	min	NOUN
njb-39	49	21	followed	follow	VERB
njb-39	49	22	by	by	ADP
njb-39	49	23	centrifugation	centrifugation	NOUN
njb-39	49	24	at	at	ADP
njb-39	49	25	8000	8000	NUM
njb-39	49	26	g	g	NOUN
njb-39	49	27	for	for	ADP
njb-39	49	28	1	1	NUM
njb-39	49	29	min	min	NOUN
njb-39	49	30	.	.	NOUN
njb-39	49	31	competent	competent	ADJ
njb-39	49	32	cells	cell	NOUN
njb-39	49	33	preparation	preparation	NOUN
njb-39	49	34	,	,	PUNCT
njb-39	49	35	ligation	ligation	NOUN
njb-39	49	36	reaction	reaction	NOUN
njb-39	49	37	and	and	CCONJ
njb-39	49	38	plasmid	plasmid	NOUN
njb-39	49	39	isolation	isolation	NOUN
njb-39	49	40	dh5	dh5	NOUN
njb-39	49	41	-	-	PUNCT
njb-39	49	42	α	α	PROPN
njb-39	49	43	,	,	PUNCT
njb-39	49	44	a	a	DET
njb-39	49	45	non	non	ADJ
njb-39	49	46	-	-	ADJ
njb-39	49	47	pathogenic	pathogenic	ADJ
njb-39	49	48	strain	strain	NOUN
njb-39	49	49	of	of	ADP
njb-39	49	50	e.coli	e.coli	NOUN
njb-39	49	51	was	be	AUX
njb-39	49	52	used	use	VERB
njb-39	49	53	for	for	ADP
njb-39	49	54	the	the	DET
njb-39	49	55	purpose	purpose	NOUN
njb-39	49	56	.	.	PUNCT
njb-39	50	1	it	it	PRON
njb-39	50	2	were	be	AUX
njb-39	50	3	grown	grow	VERB
njb-39	50	4	overnight	overnight	ADV
njb-39	50	5	in	in	ADP
njb-39	50	6	100ml	100ml	PROPN
njb-39	50	7	conical	conical	PROPN
njb-39	50	8	flask	flask	PROPN
njb-39	50	9	containing	contain	VERB
njb-39	50	10	luria	luria	PROPN
njb-39	50	11	broth	broth	NOUN
njb-39	50	12	(	(	PUNCT
njb-39	50	13	lb	lb	NOUN
njb-39	50	14	)	)	PUNCT
njb-39	50	15	.	.	PUNCT
njb-39	51	1	the	the	DET
njb-39	51	2	overnight	overnight	ADJ
njb-39	51	3	bacterial	bacterial	ADJ
njb-39	51	4	culture	culture	NOUN
njb-39	51	5	was	be	AUX
njb-39	51	6	diluted	dilute	VERB
njb-39	51	7	1/200	1/200	NUM
njb-39	51	8	to	to	ADP
njb-39	51	9	25	25	NUM
njb-39	51	10	ml	ml	NOUN
njb-39	51	11	of	of	ADP
njb-39	51	12	soc	soc	NOUN
njb-39	51	13	media	medium	NOUN
njb-39	51	14	in	in	ADP
njb-39	51	15	10x	10x	NOUN
njb-39	51	16	culture	culture	NOUN
njb-39	51	17	volume	volume	NOUN
njb-39	51	18	flasks	flask	NOUN
njb-39	51	19	(	(	PUNCT
njb-39	51	20	25	25	NUM
njb-39	51	21	ml	ml	NOUN
njb-39	51	22	in	in	ADP
njb-39	51	23	250	250	NUM
njb-39	51	24	ml	ml	PROPN
njb-39	51	25	flask	flask	NOUN
njb-39	51	26	)	)	PUNCT
njb-39	51	27	.	.	PUNCT
njb-39	52	1	flask	flask	NOUN
njb-39	52	2	containing	contain	VERB
njb-39	52	3	bacteria	bacteria	NOUN
njb-39	52	4	was	be	AUX
njb-39	52	5	grown	grow	VERB
njb-39	52	6	to	to	ADP
njb-39	52	7	early	early	ADJ
njb-39	52	8	log	log	NOUN
njb-39	52	9	phase	phase	NOUN
njb-39	52	10	in	in	ADP
njb-39	52	11	shaker	shaker	NOUN
njb-39	52	12	incubator	incubator	NOUN
njb-39	52	13	.	.	PUNCT
njb-39	53	1	the	the	DET
njb-39	53	2	growth	growth	NOUN
njb-39	53	3	of	of	ADP
njb-39	53	4	the	the	DET
njb-39	53	5	culture	culture	NOUN
njb-39	53	6	was	be	AUX
njb-39	53	7	measured	measure	VERB
njb-39	53	8	at	at	ADP
njb-39	53	9	an	an	DET
njb-39	53	10	interval	interval	NOUN
njb-39	53	11	of	of	ADP
njb-39	53	12	30	30	NUM
njb-39	53	13	minutes	minute	NOUN
njb-39	53	14	until	until	SCONJ
njb-39	53	15	an	an	DET
njb-39	53	16	od	od	NOUN
njb-39	53	17	of	of	ADP
njb-39	53	18	0.4	0.4	NUM
njb-39	53	19	at	at	ADP
njb-39	53	20	600	600	NUM
njb-39	53	21	nm	nm	NOUN
njb-39	53	22	were	be	AUX
njb-39	53	23	obtained	obtain	VERB
njb-39	53	24	.	.	PUNCT
njb-39	54	1	the	the	DET
njb-39	54	2	cells	cell	NOUN
njb-39	54	3	were	be	AUX
njb-39	54	4	collected	collect	VERB
njb-39	54	5	by	by	ADP
njb-39	54	6	centrifugation	centrifugation	NOUN
njb-39	54	7	at	at	ADP
njb-39	54	8	5000	5000	NUM
njb-39	54	9	g	g	NOUN
njb-39	54	10	for	for	ADP
njb-39	54	11	5	5	NUM
njb-39	54	12	min	min	NOUN
njb-39	54	13	in	in	ADP
njb-39	54	14	cold	cold	ADJ
njb-39	54	15	centrifuge	centrifuge	NOUN
njb-39	54	16	(	(	PUNCT
njb-39	54	17	4	4	NUM
njb-39	54	18	°	°	NUM
njb-39	54	19	c	c	NOUN
njb-39	54	20	)	)	PUNCT
njb-39	54	21	and	and	CCONJ
njb-39	54	22	were	be	AUX
njb-39	54	23	kept	keep	VERB
njb-39	54	24	on	on	ADP
njb-39	54	25	ice	ice	NOUN
njb-39	54	26	in	in	ADP
njb-39	54	27	all	all	DET
njb-39	54	28	the	the	DET
njb-39	54	29	further	further	ADJ
njb-39	54	30	steps	step	NOUN
njb-39	54	31	.	.	PUNCT
njb-39	55	1	the	the	DET
njb-39	55	2	cells	cell	NOUN
njb-39	55	3	were	be	AUX
njb-39	55	4	re	re	AUX
njb-39	55	5	suspended	suspend	VERB
njb-39	55	6	in	in	ADP
njb-39	55	7	12	12	NUM
njb-39	55	8	ml	ml	NOUN
njb-39	55	9	of	of	ADP
njb-39	55	10	0.1	0.1	NUM
njb-39	55	11	m	m	NOUN
njb-39	55	12	ice	ice	NOUN
njb-39	55	13	cold	cold	ADJ
njb-39	55	14	cacl2	cacl2	NOUN
njb-39	55	15	and	and	CCONJ
njb-39	55	16	placed	place	VERB
njb-39	55	17	on	on	ADP
njb-39	55	18	ice	ice	NOUN
njb-39	55	19	for	for	ADP
njb-39	55	20	minimum	minimum	ADJ
njb-39	55	21	30	30	NUM
njb-39	55	22	minutes	minute	NOUN
njb-39	55	23	.	.	PUNCT
njb-39	56	1	the	the	DET
njb-39	56	2	cells	cell	NOUN
njb-39	56	3	were	be	AUX
njb-39	56	4	centrifuged	centrifuge	VERB
njb-39	56	5	at	at	ADP
njb-39	56	6	5000	5000	NUM
njb-39	56	7	g	g	NOUN
njb-39	56	8	for	for	ADP
njb-39	56	9	10	10	NUM
njb-39	56	10	min	min	NOUN
njb-39	56	11	and	and	CCONJ
njb-39	56	12	the	the	DET
njb-39	56	13	pellet	pellet	NOUN
njb-39	56	14	was	be	AUX
njb-39	56	15	collected	collect	VERB
njb-39	56	16	.	.	PUNCT
njb-39	57	1	the	the	DET
njb-39	57	2	pellet	pellet	NOUN
njb-39	57	3	was	be	AUX
njb-39	57	4	further	far	ADV
njb-39	57	5	re	re	VERB
njb-39	57	6	suspended	suspend	VERB
njb-39	57	7	in	in	ADP
njb-39	57	8	1/10	1/10	NUM
njb-39	57	9	volume	volume	NOUN
njb-39	57	10	of	of	ADP
njb-39	57	11	0.1	0.1	NUM
njb-39	57	12	m	m	NUM
njb-39	57	13	cacl2	cacl2	PROPN
njb-39	57	14	.	.	PROPN
njb-39	57	15	cells	cell	NOUN
njb-39	57	16	were	be	AUX
njb-39	57	17	ready	ready	ADJ
njb-39	57	18	to	to	PART
njb-39	57	19	use	use	VERB
njb-39	57	20	as	as	ADP
njb-39	57	21	competent	competent	ADJ
njb-39	57	22	cells	cell	NOUN
njb-39	57	23	for	for	ADP
njb-39	57	24	the	the	DET
njb-39	57	25	transformation	transformation	NOUN
njb-39	57	26	experiment	experiment	NOUN
njb-39	57	27	.	.	PUNCT
njb-39	58	1	ice	ice	NOUN
njb-39	58	2	cold	cold	ADJ
njb-39	58	3	sterile	sterile	ADJ
njb-39	58	4	glycerol	glycerol	NOUN
njb-39	58	5	was	be	AUX
njb-39	58	6	added	add	VERB
njb-39	58	7	to	to	ADP
njb-39	58	8	final	final	ADJ
njb-39	58	9	concentration	concentration	NOUN
njb-39	58	10	of	of	ADP
njb-39	58	11	10	10	NUM
njb-39	58	12	%	%	NOUN
njb-39	58	13	(	(	PUNCT
njb-39	58	14	v	v	NOUN
njb-39	58	15	/	/	SYM
njb-39	58	16	v	v	NOUN
njb-39	58	17	)	)	PUNCT
njb-39	58	18	with	with	ADP
njb-39	58	19	a	a	DET
njb-39	58	20	gentle	gentle	ADJ
njb-39	58	21	mixing	mixing	NOUN
njb-39	58	22	.	.	PUNCT
njb-39	59	1	aliquots	aliquot	NOUN
njb-39	59	2	of	of	ADP
njb-39	59	3	100µl	100µl	NOUN
njb-39	59	4	of	of	ADP
njb-39	59	5	cells	cell	NOUN
njb-39	59	6	were	be	AUX
njb-39	59	7	made	make	VERB
njb-39	59	8	and	and	CCONJ
njb-39	59	9	stored	store	VERB
njb-39	59	10	at	at	ADP
njb-39	59	11	-70	-70	PROPN
njb-39	59	12	°	°	NOUN
njb-39	59	13	c	c	NOUN
njb-39	59	14	until	until	ADP
njb-39	59	15	further	further	ADJ
njb-39	59	16	use	use	NOUN
njb-39	59	17	.	.	PUNCT
njb-39	60	1	the	the	DET
njb-39	60	2	tube	tube	NOUN
njb-39	60	3	containing	contain	VERB
njb-39	60	4	the	the	DET
njb-39	60	5	pgem	pgem	PROPN
njb-39	60	6	-	-	PUNCT
njb-39	60	7	t	t	PROPN
njb-39	60	8	easy	easy	ADJ
njb-39	60	9	vector	vector	NOUN
njb-39	60	10	and	and	CCONJ
njb-39	60	11	the	the	DET
njb-39	60	12	control	control	NOUN
njb-39	60	13	insert	insert	VERB
njb-39	60	14	dna	dna	PROPN
njb-39	60	15	tubes	tube	NOUN
njb-39	60	16	were	be	AUX
njb-39	60	17	centrifuged	centrifuge	VERB
njb-39	60	18	briefly	briefly	ADV
njb-39	60	19	to	to	PART
njb-39	60	20	collect	collect	VERB
njb-39	60	21	the	the	DET
njb-39	60	22	content	content	NOUN
njb-39	60	23	at	at	ADP
njb-39	60	24	the	the	DET
njb-39	60	25	bottom	bottom	NOUN
njb-39	60	26	of	of	ADP
njb-39	60	27	the	the	DET
njb-39	60	28	tube	tube	NOUN
njb-39	60	29	.	.	PUNCT
njb-39	61	1	the	the	DET
njb-39	61	2	2x	2x	NUM
njb-39	61	3	rapid	rapid	ADJ
njb-39	61	4	ligation	ligation	NOUN
njb-39	61	5	buffer	buffer	NOUN
njb-39	61	6	was	be	AUX
njb-39	61	7	vigorously	vigorously	ADV
njb-39	61	8	vortexed	vortexe	VERB
njb-39	61	9	before	before	ADP
njb-39	61	10	use	use	NOUN
njb-39	61	11	.	.	PUNCT
njb-39	62	1	ligation	ligation	NOUN
njb-39	62	2	reaction	reaction	NOUN
njb-39	62	3	was	be	AUX
njb-39	62	4	prepared	prepared	ADJ
njb-39	62	5	and	and	CCONJ
njb-39	62	6	reaction	reaction	NOUN
njb-39	62	7	mixture	mixture	NOUN
njb-39	62	8	was	be	AUX
njb-39	62	9	mixed	mixed	ADJ
njb-39	62	10	properly	properly	ADV
njb-39	62	11	by	by	ADP
njb-39	62	12	pipetting	pipette	VERB
njb-39	62	13	and	and	CCONJ
njb-39	62	14	was	be	AUX
njb-39	62	15	incubated	incubate	VERB
njb-39	62	16	for	for	ADP
njb-39	62	17	30	30	NUM
njb-39	62	18	min	min	NOUN
njb-39	62	19	at	at	ADP
njb-39	62	20	4	4	NUM
njb-39	62	21	°	°	NOUN
njb-39	62	22	c	c	NOUN
njb-39	62	23	for	for	ADP
njb-39	62	24	optimal	optimal	ADJ
njb-39	62	25	ligation	ligation	NOUN
njb-39	62	26	.	.	PUNCT
njb-39	63	1	two	two	NUM
njb-39	63	2	µl	µl	NOUN
njb-39	63	3	of	of	ADP
njb-39	63	4	the	the	DET
njb-39	63	5	ligation	ligation	NOUN
njb-39	63	6	reaction	reaction	NOUN
njb-39	63	7	were	be	AUX
njb-39	63	8	added	add	VERB
njb-39	63	9	to	to	ADP
njb-39	63	10	the	the	DET
njb-39	63	11	eppendorf	eppendorf	PROPN
njb-39	63	12	tube	tube	NOUN
njb-39	63	13	containing	contain	VERB
njb-39	63	14	100	100	NUM
njb-39	63	15	µl	µl	NOUN
njb-39	63	16	of	of	ADP
njb-39	63	17	the	the	DET
njb-39	63	18	competent	competent	ADJ
njb-39	63	19	cells	cell	NOUN
njb-39	63	20	.	.	PUNCT
njb-39	64	1	the	the	DET
njb-39	64	2	transformation	transformation	NOUN
njb-39	64	3	tube	tube	NOUN
njb-39	64	4	containing	contain	VERB
njb-39	64	5	ligation	ligation	NOUN
njb-39	64	6	reaction	reaction	NOUN
njb-39	64	7	mixture	mixture	NOUN
njb-39	64	8	and	and	CCONJ
njb-39	64	9	competent	competent	ADJ
njb-39	64	10	cells	cell	NOUN
njb-39	64	11	were	be	AUX
njb-39	64	12	given	give	VERB
njb-39	64	13	heat	heat	NOUN
njb-39	64	14	shock	shock	NOUN
njb-39	64	15	at	at	ADP
njb-39	64	16	42	42	NUM
njb-39	64	17	°	°	NOUN
njb-39	64	18	c	c	NOUN
njb-39	64	19	for	for	ADP
njb-39	64	20	40	40	NUM
njb-39	64	21	-	-	SYM
njb-39	64	22	50	50	NUM
njb-39	64	23	second	second	NOUN
njb-39	64	24	and	and	CCONJ
njb-39	64	25	was	be	AUX
njb-39	64	26	immediately	immediately	ADV
njb-39	64	27	returned	return	VERB
njb-39	64	28	to	to	ADP
njb-39	64	29	the	the	DET
njb-39	64	30	ice	ice	NOUN
njb-39	64	31	bucket	bucket	NOUN
njb-39	64	32	.	.	PUNCT
njb-39	65	1	950µl	950µl	PROPN
njb-39	65	2	of	of	ADP
njb-39	65	3	the	the	DET
njb-39	65	4	soc	soc	NOUN
njb-39	65	5	media	medium	NOUN
njb-39	65	6	containing	contain	VERB
njb-39	65	7	antibiotic	antibiotic	ADJ
njb-39	65	8	was	be	AUX
njb-39	65	9	added	add	VERB
njb-39	65	10	to	to	ADP
njb-39	65	11	the	the	DET
njb-39	65	12	above	above	ADJ
njb-39	65	13	tube	tube	NOUN
njb-39	65	14	and	and	CCONJ
njb-39	65	15	were	be	AUX
njb-39	65	16	incubated	incubate	VERB
njb-39	65	17	in	in	ADP
njb-39	65	18	a	a	DET
njb-39	65	19	shaking	shake	VERB
njb-39	65	20	incubator	incubator	NOUN
njb-39	65	21	for	for	ADP
njb-39	65	22	1.5	1.5	NUM
njb-39	65	23	hrs	hr	NOUN
njb-39	65	24	at	at	ADP
njb-39	65	25	37	37	NUM
njb-39	65	26	°	°	NOUN
njb-39	65	27	c	c	NOUN
njb-39	65	28	with	with	ADP
njb-39	65	29	shaking	shake	VERB
njb-39	65	30	(	(	PUNCT
njb-39	65	31	~150	~150	NUM
njb-39	65	32	rpm	rpm	NOUN
njb-39	65	33	)	)	PUNCT
njb-39	65	34	.	.	PUNCT
njb-39	66	1	100	100	NUM
njb-39	66	2	µl	µl	NOUN
njb-39	66	3	of	of	ADP
njb-39	66	4	the	the	DET
njb-39	66	5	transformation	transformation	NOUN
njb-39	66	6	culture	culture	NOUN
njb-39	66	7	was	be	AUX
njb-39	66	8	plated	plate	VERB
njb-39	66	9	onto	onto	ADP
njb-39	66	10	duplicate	duplicate	PROPN
njb-39	66	11	lb	lb	NOUN
njb-39	66	12	/	/	SYM
njb-39	66	13	ampicillin	ampicillin	NOUN
njb-39	66	14	/	/	SYM
njb-39	66	15	iptg	iptg	PROPN
njb-39	66	16	/	/	SYM
njb-39	66	17	x	x	ADJ
njb-39	66	18	-	-	ADJ
njb-39	66	19	gal	gal	ADJ
njb-39	66	20	plates	plate	NOUN
njb-39	66	21	.	.	PUNCT
njb-39	67	1	the	the	DET
njb-39	67	2	plates	plate	NOUN
njb-39	67	3	were	be	AUX
njb-39	67	4	incubated	incubate	VERB
njb-39	67	5	overnight	overnight	ADV
njb-39	67	6	at	at	ADP
njb-39	67	7	37	37	NUM
njb-39	67	8	°	°	NOUN
njb-39	67	9	c	c	NOUN
njb-39	67	10	and	and	CCONJ
njb-39	67	11	were	be	AUX
njb-39	67	12	screened	screen	VERB
njb-39	67	13	for	for	ADP
njb-39	67	14	blue	blue	ADJ
njb-39	67	15	and	and	CCONJ
njb-39	67	16	white	white	ADJ
njb-39	67	17	colonies	colony	NOUN
njb-39	67	18	.	.	PUNCT
njb-39	68	1	the	the	DET
njb-39	68	2	white	white	ADJ
njb-39	68	3	colonies	colony	NOUN
njb-39	68	4	were	be	AUX
njb-39	68	5	the	the	DET
njb-39	68	6	desired	desire	VERB
njb-39	68	7	insert	insert	NOUN
njb-39	68	8	.	.	PUNCT
njb-39	69	1	to	to	PART
njb-39	69	2	facilitate	facilitate	VERB
njb-39	69	3	the	the	DET
njb-39	69	4	blue	blue	ADJ
njb-39	69	5	color	color	NOUN
njb-39	69	6	development	development	NOUN
njb-39	69	7	,	,	PUNCT
njb-39	69	8	plates	plate	NOUN
njb-39	69	9	were	be	AUX
njb-39	69	10	stored	store	VERB
njb-39	69	11	at	at	ADP
njb-39	69	12	4	4	NUM
njb-39	69	13	°	°	NOUN
njb-39	69	14	c	c	NOUN
njb-39	69	15	(	(	PUNCT
njb-39	69	16	after	after	ADP
njb-39	69	17	37	37	NUM
njb-39	69	18	°	°	NOUN
njb-39	69	19	c	c	NOUN
njb-39	69	20	overnight	overnight	PROPN
njb-39	69	21	incubation	incubation	NOUN
njb-39	69	22	)	)	PUNCT
njb-39	69	23	.	.	PUNCT
njb-39	70	1	finally	finally	ADV
njb-39	70	2	the	the	DET
njb-39	70	3	white	white	ADJ
njb-39	70	4	colonies	colony	NOUN
njb-39	70	5	,	,	PUNCT
njb-39	70	6	supposed	suppose	VERB
njb-39	70	7	to	to	PART
njb-39	70	8	contain	contain	VERB
njb-39	70	9	the	the	DET
njb-39	70	10	vector	vector	NOUN
njb-39	70	11	with	with	ADP
njb-39	70	12	the	the	DET
njb-39	70	13	desired	desire	VERB
njb-39	70	14	insert	insert	NOUN
njb-39	70	15	were	be	AUX
njb-39	70	16	selected	select	VERB
njb-39	70	17	and	and	CCONJ
njb-39	70	18	were	be	AUX
njb-39	70	19	grown	grow	VERB
njb-39	70	20	in	in	ADP
njb-39	70	21	10	10	NUM
njb-39	70	22	ml	ml	NOUN
njb-39	70	23	of	of	ADP
njb-39	70	24	lb	lb	NOUN
njb-39	70	25	containing	contain	VERB
njb-39	70	26	100	100	NUM
njb-39	70	27	µg	µg	PROPN
njb-39	70	28	/	/	SYM
njb-39	70	29	ml	ml	PROPN
njb-39	70	30	ampicillin	ampicillin	PROPN
njb-39	70	31	,	,	PUNCT
njb-39	70	32	overnight	overnight	ADV
njb-39	70	33	in	in	ADP
njb-39	70	34	a	a	DET
njb-39	70	35	shaker	shaker	NOUN
njb-39	70	36	incubator	incubator	NOUN
njb-39	70	37	at	at	ADP
njb-39	70	38	37	37	NUM
njb-39	70	39	°	°	NOUN
njb-39	70	40	c	c	NOUN
njb-39	70	41	at	at	ADP
njb-39	70	42	speed	speed	NOUN
njb-39	70	43	of	of	ADP
njb-39	70	44	250rpm	250rpm	NUM
njb-39	70	45	.	.	PUNCT
njb-39	71	1	the	the	DET
njb-39	71	2	bacterial	bacterial	ADJ
njb-39	71	3	cells	cell	NOUN
njb-39	71	4	grown	grow	VERB
njb-39	71	5	in	in	ADP
njb-39	71	6	the	the	DET
njb-39	71	7	lb	lb	NOUN
njb-39	71	8	medium	medium	NOUN
njb-39	71	9	by	by	ADP
njb-39	71	10	addition	addition	NOUN
njb-39	71	11	of	of	ADP
njb-39	71	12	antibiotic	antibiotic	ADJ
njb-39	71	13	were	be	AUX
njb-39	71	14	harvested	harvest	VERB
njb-39	71	15	by	by	ADP
njb-39	71	16	centrifugation	centrifugation	NOUN
njb-39	71	17	at	at	ADP
njb-39	71	18	6000	6000	NUM
njb-39	71	19	g	g	NOUN
njb-39	71	20	for	for	ADP
njb-39	71	21	15	15	NUM
njb-39	71	22	min	min	NOUN
njb-39	71	23	at	at	ADP
njb-39	71	24	4	4	NUM
njb-39	71	25	°	°	NOUN
njb-39	71	26	c	c	NOUN
njb-39	71	27	.	.	PUNCT
njb-39	72	1	the	the	DET
njb-39	72	2	cell	cell	NOUN
njb-39	72	3	pellet	pellet	NOUN
njb-39	72	4	was	be	AUX
njb-39	72	5	resuspended	resuspend	VERB
njb-39	72	6	in	in	ADP
njb-39	72	7	0.3	0.3	NUM
njb-39	72	8	ml	ml	NOUN
njb-39	72	9	of	of	ADP
njb-39	72	10	buffer	buffer	PROPN
njb-39	72	11	p1	p1	PROPN
njb-39	72	12	.	.	PROPN
njb-39	72	13	300µl	300µl	PROPN
njb-39	72	14	of	of	ADP
njb-39	72	15	buffer	buffer	NOUN
njb-39	72	16	p2	p2	PROPN
njb-39	72	17	was	be	AUX
njb-39	72	18	added	add	VERB
njb-39	72	19	and	and	CCONJ
njb-39	72	20	mixed	mix	VERB
njb-39	72	21	thoroughly	thoroughly	ADV
njb-39	72	22	and	and	CCONJ
njb-39	72	23	vigorously	vigorously	ADV
njb-39	72	24	and	and	CCONJ
njb-39	72	25	were	be	AUX
njb-39	72	26	incubated	incubate	VERB
njb-39	72	27	at	at	ADP
njb-39	72	28	room	room	NOUN
njb-39	72	29	temperature	temperature	NOUN
njb-39	72	30	(	(	PUNCT
njb-39	72	31	15	15	NUM
njb-39	72	32	-	-	SYM
njb-39	72	33	25	25	NUM
njb-39	72	34	°	°	NOUN
njb-39	72	35	c	c	NOUN
njb-39	72	36	)	)	PUNCT
njb-39	72	37	for	for	ADP
njb-39	72	38	5	5	NUM
njb-39	72	39	min	min	PROPN
njb-39	72	40	.	.	PROPN
njb-39	72	41	300µl	300µl	PROPN
njb-39	72	42	of	of	ADP
njb-39	72	43	chilled	chilled	ADJ
njb-39	72	44	buffer	buffer	NOUN
njb-39	72	45	p3	p3	PROPN
njb-39	72	46	was	be	AUX
njb-39	72	47	added	add	VERB
njb-39	72	48	followed	follow	VERB
njb-39	72	49	by	by	ADP
njb-39	72	50	mixing	mix	VERB
njb-39	72	51	vigorously	vigorously	ADV
njb-39	72	52	inverting	invert	VERB
njb-39	72	53	the	the	DET
njb-39	72	54	tube	tube	NOUN
njb-39	72	55	(	(	PUNCT
njb-39	72	56	8	8	NUM
njb-39	72	57	-	-	SYM
njb-39	72	58	10	10	NUM
njb-39	72	59	times	time	NOUN
njb-39	72	60	)	)	PUNCT
njb-39	72	61	and	and	CCONJ
njb-39	72	62	were	be	AUX
njb-39	72	63	incubated	incubate	VERB
njb-39	72	64	on	on	ADP
njb-39	72	65	ice	ice	NOUN
njb-39	72	66	for	for	ADP
njb-39	72	67	5	5	NUM
njb-39	72	68	min	min	NOUN
njb-39	72	69	.	.	PROPN
njb-39	72	70	mixture	mixture	NOUN
njb-39	72	71	was	be	AUX
njb-39	72	72	nepal	nepal	ADJ
njb-39	72	73	journal	journal	NOUN
njb-39	72	74	of	of	ADP
njb-39	72	75	biotechnology	biotechnology	NOUN
njb-39	72	76	.	.	PUNCT
njb-39	73	1	d	d	X
njb-39	73	2	e	e	PROPN
njb-39	73	3	c	c	PROPN
njb-39	73	4	.	.	PUNCT
njb-39	74	1	2	2	NUM
njb-39	74	2	0	0	NUM
njb-39	74	3	1	1	NUM
njb-39	74	4	5	5	NUM
njb-39	74	5	vol	vol	NOUN
njb-39	74	6	.	.	PUNCT
njb-39	75	1	3	3	NUM
njb-39	75	2	,	,	PUNCT
njb-39	75	3	no	no	NOUN
njb-39	75	4	.	.	NOUN
njb-39	75	5	1:15	1:15	NUM
njb-39	75	6	-	-	SYM
njb-39	75	7	21	21	NUM
njb-39	75	8	jha	jha	NOUN
njb-39	75	9	et	et	PROPN
njb-39	75	10	al	al	PROPN
njb-39	75	11	.	.	PUNCT
njb-39	76	1	©	©	PROPN
njb-39	76	2	njb	njb	PROPN
njb-39	76	3	,	,	PUNCT
njb-39	76	4	biotechnology	biotechnology	NOUN
njb-39	76	5	society	society	NOUN
njb-39	76	6	of	of	ADP
njb-39	76	7	nepal	nepal	ADJ
njb-39	76	8	17	17	NUM
njb-39	76	9	nepjol.info/index.php/njb	nepjol.info/index.php/njb	PROPN
njb-39	76	10	centrifuged	centrifuge	VERB
njb-39	76	11	at	at	ADP
njb-39	76	12	10,000	10,000	NUM
njb-39	76	13	g	g	NOUN
njb-39	76	14	in	in	ADP
njb-39	76	15	a	a	DET
njb-39	76	16	micro	micro	NOUN
njb-39	76	17	centrifuge	centrifuge	NOUN
njb-39	76	18	for	for	ADP
njb-39	76	19	10	10	NUM
njb-39	76	20	min	min	NOUN
njb-39	76	21	.	.	PUNCT
njb-39	77	1	the	the	DET
njb-39	77	2	supernatant	supernatant	NOUN
njb-39	77	3	containing	contain	VERB
njb-39	77	4	the	the	DET
njb-39	77	5	plasmid	plasmid	ADJ
njb-39	77	6	dna	dna	PROPN
njb-39	77	7	were	be	AUX
njb-39	77	8	aspirated	aspirate	VERB
njb-39	77	9	out	out	ADP
njb-39	77	10	carefully	carefully	ADV
njb-39	77	11	.	.	PUNCT
njb-39	78	1	during	during	ADP
njb-39	78	2	the	the	DET
njb-39	78	3	mean	mean	ADJ
njb-39	78	4	time	time	NOUN
njb-39	78	5	qiagen	qiagen	PROPN
njb-39	78	6	-	-	PUNCT
njb-39	78	7	tip	tip	NOUN
njb-39	78	8	20	20	NUM
njb-39	78	9	was	be	AUX
njb-39	78	10	equilibrated	equilibrate	VERB
njb-39	78	11	by	by	ADP
njb-39	78	12	applying	apply	VERB
njb-39	78	13	1ml	1ml	NOUN
njb-39	78	14	of	of	ADP
njb-39	78	15	the	the	DET
njb-39	78	16	buffer	buffer	NOUN
njb-39	78	17	qbt	qbt	PROPN
njb-39	78	18	,	,	PUNCT
njb-39	78	19	and	and	CCONJ
njb-39	78	20	was	be	AUX
njb-39	78	21	allowed	allow	VERB
njb-39	78	22	to	to	PART
njb-39	78	23	empty	empty	VERB
njb-39	78	24	the	the	DET
njb-39	78	25	column	column	NOUN
njb-39	78	26	by	by	ADP
njb-39	78	27	gravity	gravity	NOUN
njb-39	78	28	flow	flow	NOUN
njb-39	78	29	.	.	PUNCT
njb-39	79	1	the	the	DET
njb-39	79	2	supernatant	supernatant	NOUN
njb-39	79	3	obtained	obtain	VERB
njb-39	79	4	after	after	SCONJ
njb-39	79	5	centrifugation	centrifugation	NOUN
njb-39	79	6	was	be	AUX
njb-39	79	7	applied	apply	VERB
njb-39	79	8	to	to	ADP
njb-39	79	9	the	the	DET
njb-39	79	10	qiagen	qiagen	PROPN
njb-39	79	11	-	-	PUNCT
njb-39	79	12	tip	tip	NOUN
njb-39	79	13	20	20	NUM
njb-39	79	14	and	and	CCONJ
njb-39	79	15	allowed	allow	VERB
njb-39	79	16	to	to	PART
njb-39	79	17	enter	enter	VERB
njb-39	79	18	the	the	DET
njb-39	79	19	resin	resin	NOUN
njb-39	79	20	by	by	ADP
njb-39	79	21	gravity	gravity	NOUN
njb-39	79	22	flow	flow	NOUN
njb-39	79	23	.	.	PUNCT
njb-39	80	1	the	the	PRON
njb-39	80	2	qiagen	qiagen	PROPN
njb-39	80	3	-	-	PUNCT
njb-39	80	4	tip	tip	NOUN
njb-39	80	5	20	20	NUM
njb-39	80	6	were	be	AUX
njb-39	80	7	washed	wash	VERB
njb-39	80	8	with	with	ADP
njb-39	80	9	2x2	2x2	NUM
njb-39	80	10	ml	ml	NOUN
njb-39	80	11	of	of	ADP
njb-39	80	12	buffer	buffer	PROPN
njb-39	80	13	qc	qc	PROPN
njb-39	80	14	,	,	PUNCT
njb-39	80	15	followed	follow	VERB
njb-39	80	16	by	by	ADP
njb-39	80	17	elution	elution	NOUN
njb-39	80	18	of	of	ADP
njb-39	80	19	dna	dna	PROPN
njb-39	80	20	with	with	ADP
njb-39	80	21	0.8ml	0.8ml	NUM
njb-39	80	22	of	of	ADP
njb-39	80	23	buffer	buffer	PROPN
njb-39	80	24	qf	qf	PROPN
njb-39	80	25	.	.	PUNCT
njb-39	81	1	the	the	DET
njb-39	81	2	eluted	elute	VERB
njb-39	81	3	dna	dna	PROPN
njb-39	81	4	was	be	AUX
njb-39	81	5	precipitated	precipitate	VERB
njb-39	81	6	by	by	ADP
njb-39	81	7	adding	add	VERB
njb-39	81	8	isopropanol	isopropanol	NOUN
njb-39	81	9	at	at	ADP
njb-39	81	10	room	room	NOUN
njb-39	81	11	temperature	temperature	NOUN
njb-39	81	12	.	.	PUNCT
njb-39	82	1	(	(	PUNCT
njb-39	82	2	0.56ml	0.56ml	NOUN
njb-39	82	3	per	per	ADP
njb-39	82	4	0.8ml	0.8ml	NUM
njb-39	82	5	of	of	ADP
njb-39	82	6	elution	elution	NOUN
njb-39	82	7	volume	volume	NOUN
njb-39	82	8	)	)	PUNCT
njb-39	82	9	.	.	PUNCT
njb-39	83	1	the	the	DET
njb-39	83	2	mixture	mixture	NOUN
njb-39	83	3	was	be	AUX
njb-39	83	4	be	be	AUX
njb-39	83	5	then	then	ADV
njb-39	83	6	mixed	mixed	ADJ
njb-39	83	7	and	and	CCONJ
njb-39	83	8	centrifuged	centrifuge	VERB
njb-39	83	9	immediately	immediately	ADV
njb-39	83	10	at	at	ADP
njb-39	83	11	≥	≥	NOUN
njb-39	83	12	10,000rpm	10,000rpm	NUM
njb-39	83	13	for	for	ADP
njb-39	83	14	30	30	NUM
njb-39	83	15	min	min	NOUN
njb-39	83	16	in	in	ADP
njb-39	83	17	a	a	DET
njb-39	83	18	micro	micro	NOUN
njb-39	83	19	centrifuge	centrifuge	NOUN
njb-39	83	20	at	at	ADP
njb-39	83	21	4	4	NUM
njb-39	83	22	°	°	NOUN
njb-39	83	23	c	c	NOUN
njb-39	83	24	and	and	CCONJ
njb-39	83	25	the	the	DET
njb-39	83	26	supernatant	supernatant	NOUN
njb-39	83	27	was	be	AUX
njb-39	83	28	be	be	AUX
njb-39	83	29	decanted	decant	VERB
njb-39	83	30	carefully	carefully	ADV
njb-39	83	31	.	.	PUNCT
njb-39	84	1	the	the	DET
njb-39	84	2	residual	residual	ADJ
njb-39	84	3	dna	dna	PROPN
njb-39	84	4	pellet	pellet	NOUN
njb-39	84	5	were	be	AUX
njb-39	84	6	washed	wash	VERB
njb-39	84	7	with	with	ADP
njb-39	84	8	1ml	1ml	NOUN
njb-39	84	9	of	of	ADP
njb-39	84	10	70	70	NUM
njb-39	84	11	%	%	NOUN
njb-39	84	12	ethanol	ethanol	NOUN
njb-39	84	13	at	at	ADP
njb-39	84	14	least	least	ADJ
njb-39	84	15	thrice	thrice	ADV
njb-39	84	16	by	by	ADP
njb-39	84	17	centrifuging	centrifuge	VERB
njb-39	84	18	the	the	DET
njb-39	84	19	pellet	pellet	NOUN
njb-39	84	20	at	at	ADP
njb-39	84	21	10,000rpm	10,000rpm	NUM
njb-39	84	22	for	for	ADP
njb-39	84	23	10	10	NUM
njb-39	84	24	min	min	NOUN
njb-39	84	25	each	each	PRON
njb-39	84	26	.	.	PUNCT
njb-39	85	1	the	the	DET
njb-39	85	2	supernatant	supernatant	NOUN
njb-39	85	3	was	be	AUX
njb-39	85	4	aspirated	aspirate	VERB
njb-39	85	5	carefully	carefully	ADV
njb-39	85	6	without	without	ADP
njb-39	85	7	disturbing	disturb	VERB
njb-39	85	8	the	the	DET
njb-39	85	9	pellet	pellet	NOUN
njb-39	85	10	.	.	PUNCT
njb-39	86	1	the	the	DET
njb-39	86	2	pellet	pellet	NOUN
njb-39	86	3	was	be	AUX
njb-39	86	4	air	air	NOUN
njb-39	86	5	dried	dry	VERB
njb-39	86	6	for	for	ADP
njb-39	86	7	5	5	NUM
njb-39	86	8	-	-	SYM
njb-39	86	9	10	10	NUM
njb-39	86	10	min	min	NOUN
njb-39	86	11	,	,	PUNCT
njb-39	86	12	and	and	CCONJ
njb-39	86	13	was	be	AUX
njb-39	86	14	re	re	VERB
njb-39	86	15	-	-	VERB
njb-39	86	16	dissolved	dissolve	VERB
njb-39	86	17	in	in	ADP
njb-39	86	18	100µl	100µl	NOUN
njb-39	86	19	of	of	ADP
njb-39	86	20	te	te	PROPN
njb-39	86	21	buffer	buffer	NOUN
njb-39	86	22	,	,	PUNCT
njb-39	86	23	ph	ph	NOUN
njb-39	86	24	8.0	8.0	NUM
njb-39	86	25	.	.	PUNCT
njb-39	87	1	virus	virus	PROPN
njb-39	87	2	revival	revival	NOUN
njb-39	87	3	,	,	PUNCT
njb-39	87	4	titration	titration	NOUN
njb-39	87	5	and	and	CCONJ
njb-39	87	6	viral	viral	ADJ
njb-39	87	7	load	load	NOUN
njb-39	87	8	in	in	ADP
njb-39	87	9	lyophilized	lyophilize	VERB
njb-39	87	10	adenovirus	adenovirus	NOUN
njb-39	87	11	lyophilized	lyophilize	VERB
njb-39	87	12	virus	virus	NOUN
njb-39	87	13	was	be	AUX
njb-39	87	14	revived	revive	VERB
njb-39	87	15	in	in	ADP
njb-39	87	16	sterile	sterile	ADJ
njb-39	87	17	conditions	condition	NOUN
njb-39	87	18	using	use	VERB
njb-39	87	19	sterile	sterile	ADJ
njb-39	87	20	double	double	ADJ
njb-39	87	21	-	-	PUNCT
njb-39	87	22	distilled	distil	VERB
njb-39	87	23	water	water	NOUN
njb-39	87	24	.	.	PUNCT
njb-39	88	1	the	the	DET
njb-39	88	2	virus	virus	NOUN
njb-39	88	3	ampoule	ampoule	NOUN
njb-39	88	4	was	be	AUX
njb-39	88	5	broken	break	VERB
njb-39	88	6	with	with	ADP
njb-39	88	7	a	a	DET
njb-39	88	8	clean	clean	ADJ
njb-39	88	9	metal	metal	NOUN
njb-39	88	10	rod	rod	NOUN
njb-39	88	11	in	in	ADP
njb-39	88	12	one	one	NUM
njb-39	88	13	strike	strike	NOUN
njb-39	88	14	at	at	ADP
njb-39	88	15	the	the	DET
njb-39	88	16	neck	neck	NOUN
njb-39	88	17	region	region	NOUN
njb-39	88	18	of	of	ADP
njb-39	88	19	the	the	DET
njb-39	88	20	ampoule	ampoule	NOUN
njb-39	88	21	in	in	ADP
njb-39	88	22	the	the	DET
njb-39	88	23	biosafety	biosafety	NOUN
njb-39	88	24	cabinet	cabinet	NOUN
njb-39	88	25	.	.	PUNCT
njb-39	89	1	1ml	1ml	ADJ
njb-39	89	2	sterile	sterile	ADJ
njb-39	89	3	water	water	NOUN
njb-39	89	4	were	be	AUX
njb-39	89	5	added	add	VERB
njb-39	89	6	to	to	ADP
njb-39	89	7	the	the	DET
njb-39	89	8	dried	dry	VERB
njb-39	89	9	virus	virus	NOUN
njb-39	89	10	and	and	CCONJ
njb-39	89	11	mixed	mix	VERB
njb-39	89	12	by	by	ADP
njb-39	89	13	pipetting	pipette	VERB
njb-39	89	14	atleast	atleast	ADJ
njb-39	89	15	20	20	NUM
njb-39	89	16	times	time	NOUN
njb-39	89	17	.	.	PUNCT
njb-39	90	1	the	the	DET
njb-39	90	2	revived	revived	ADJ
njb-39	90	3	virus	virus	NOUN
njb-39	90	4	was	be	AUX
njb-39	90	5	kept	keep	VERB
njb-39	90	6	at	at	ADP
njb-39	90	7	4	4	NUM
njb-39	90	8	°	°	NOUN
njb-39	90	9	c	c	NOUN
njb-39	90	10	for	for	ADP
njb-39	90	11	2h	2h	NUM
njb-39	90	12	so	so	SCONJ
njb-39	90	13	as	as	SCONJ
njb-39	90	14	to	to	PART
njb-39	90	15	acclimatize	acclimatize	VERB
njb-39	90	16	virus	virus	NOUN
njb-39	90	17	.	.	PUNCT
njb-39	91	1	the	the	DET
njb-39	91	2	virus	virus	NOUN
njb-39	91	3	was	be	AUX
njb-39	91	4	aliquoted	aliquote	VERB
njb-39	91	5	and	and	CCONJ
njb-39	91	6	further	far	ADV
njb-39	91	7	stored	store	VERB
njb-39	91	8	at	at	ADP
njb-39	91	9	-70	-70	PROPN
njb-39	91	10	°	°	NUM
njb-39	91	11	c	c	NOUN
njb-39	91	12	.	.	PUNCT
njb-39	92	1	to	to	PART
njb-39	92	2	determine	determine	VERB
njb-39	92	3	the	the	DET
njb-39	92	4	effect	effect	NOUN
njb-39	92	5	of	of	ADP
njb-39	92	6	lyophilization	lyophilization	NOUN
njb-39	92	7	on	on	ADP
njb-39	92	8	virus	virus	NOUN
njb-39	92	9	titer	titer	NOUN
njb-39	92	10	the	the	DET
njb-39	92	11	lyophilized	lyophilized	ADJ
njb-39	92	12	virus	virus	NOUN
njb-39	92	13	after	after	SCONJ
njb-39	92	14	revival	revival	NOUN
njb-39	92	15	was	be	AUX
njb-39	92	16	titrated	titrate	VERB
njb-39	92	17	in	in	ADP
njb-39	92	18	hep-2	hep-2	PUNCT
njb-39	92	19	cell	cell	NOUN
njb-39	92	20	line	line	NOUN
njb-39	92	21	and	and	CCONJ
njb-39	92	22	virus	virus	NOUN
njb-39	92	23	titer	titer	NOUN
njb-39	92	24	was	be	AUX
njb-39	92	25	determined	determine	VERB
njb-39	92	26	by	by	ADP
njb-39	92	27	tcid50	tcid50	NOUN
njb-39	92	28	and	and	CCONJ
njb-39	92	29	pfu	pfu	PROPN
njb-39	92	30	following	follow	VERB
njb-39	92	31	the	the	DET
njb-39	92	32	same	same	ADJ
njb-39	92	33	procedure	procedure	NOUN
njb-39	92	34	as	as	SCONJ
njb-39	92	35	described	describe	VERB
njb-39	92	36	earlier	early	ADV
njb-39	92	37	for	for	ADP
njb-39	92	38	the	the	DET
njb-39	92	39	stock	stock	NOUN
njb-39	92	40	virus	virus	NOUN
njb-39	92	41	.	.	PUNCT
njb-39	93	1	to	to	PART
njb-39	93	2	determine	determine	VERB
njb-39	93	3	the	the	DET
njb-39	93	4	effect	effect	NOUN
njb-39	93	5	of	of	ADP
njb-39	93	6	lyophilization	lyophilization	NOUN
njb-39	93	7	on	on	ADP
njb-39	93	8	adenoviral	adenoviral	ADJ
njb-39	93	9	load	load	NOUN
njb-39	93	10	the	the	DET
njb-39	93	11	lyophilized	lyophilize	VERB
njb-39	93	12	adenovirus	adenovirus	NOUN
njb-39	93	13	was	be	AUX
njb-39	93	14	be	be	AUX
njb-39	93	15	subjected	subject	VERB
njb-39	93	16	to	to	ADP
njb-39	93	17	real	real	ADJ
njb-39	93	18	time	time	NOUN
njb-39	93	19	pcr	pcr	NOUN
njb-39	93	20	as	as	SCONJ
njb-39	93	21	described	describe	VERB
njb-39	93	22	earlier	early	ADV
njb-39	93	23	and	and	CCONJ
njb-39	93	24	the	the	DET
njb-39	93	25	ct	ct	PROPN
njb-39	93	26	values	value	NOUN
njb-39	93	27	obtained	obtain	VERB
njb-39	93	28	was	be	AUX
njb-39	93	29	extrapolated	extrapolate	VERB
njb-39	93	30	with	with	ADP
njb-39	93	31	standard	standard	ADJ
njb-39	93	32	curve	curve	NOUN
njb-39	93	33	to	to	PART
njb-39	93	34	estimate	estimate	VERB
njb-39	93	35	the	the	DET
njb-39	93	36	viral	viral	ADJ
njb-39	93	37	load	load	NOUN
njb-39	93	38	.	.	PUNCT
njb-39	94	1	statistical	statistical	ADJ
njb-39	94	2	analysis	analysis	NOUN
njb-39	94	3	statistical	statistical	ADJ
njb-39	94	4	analysis	analysis	NOUN
njb-39	94	5	of	of	ADP
njb-39	94	6	significance	significance	NOUN
njb-39	94	7	was	be	AUX
njb-39	94	8	undertaken	undertake	VERB
njb-39	94	9	by	by	ADP
njb-39	94	10	a	a	DET
njb-39	94	11	paired	pair	VERB
njb-39	94	12	sample	sample	NOUN
njb-39	94	13	t	t	NOUN
njb-39	94	14	-	-	PUNCT
njb-39	94	15	test	test	NOUN
njb-39	94	16	using	use	VERB
njb-39	94	17	spss/	spss/	NUM
njb-39	94	18	qq	qq	NOUN
njb-39	94	19	graph	graph	NOUN
njb-39	94	20	pad	pad	NOUN
njb-39	94	21	prism	prism	NOUN
njb-39	94	22	software	software	NOUN
njb-39	94	23	with	with	ADP
njb-39	94	24	a	a	DET
njb-39	94	25	value	value	NOUN
njb-39	94	26	of	of	ADP
njb-39	94	27	p<0.05	p<0.05	NOUN
njb-39	94	28	for	for	ADP
njb-39	94	29	significance	significance	NOUN
njb-39	94	30	.	.	PUNCT
njb-39	95	1	result	result	PROPN
njb-39	95	2	confirmation	confirmation	NOUN
njb-39	95	3	of	of	ADP
njb-39	95	4	stock	stock	NOUN
njb-39	95	5	virus	virus	NOUN
njb-39	95	6	adenovirus	adenovirus	NOUN
njb-39	95	7	reference	reference	NOUN
njb-39	95	8	strain	strain	NOUN
njb-39	95	9	(	(	PUNCT
njb-39	95	10	kindly	kindly	ADV
njb-39	95	11	obtained	obtain	VERB
njb-39	95	12	from	from	ADP
njb-39	95	13	department	department	PROPN
njb-39	95	14	of	of	ADP
njb-39	95	15	virology	virology	PROPN
njb-39	95	16	,	,	PUNCT
njb-39	95	17	pgimer	pgimer	PROPN
njb-39	95	18	,	,	PUNCT
njb-39	95	19	chandigarh	chandigarh	PROPN
njb-39	95	20	)	)	PUNCT
njb-39	95	21	was	be	AUX
njb-39	95	22	successfully	successfully	ADV
njb-39	95	23	propagated	propagate	VERB
njb-39	95	24	in	in	ADP
njb-39	95	25	(	(	PUNCT
njb-39	95	26	hep-2	hep-2	SYM
njb-39	95	27	)	)	PUNCT
njb-39	95	28	cells	cell	NOUN
njb-39	95	29	.	.	PUNCT
njb-39	96	1	cytopathic	cytopathic	ADJ
njb-39	96	2	effect	effect	NOUN
njb-39	96	3	(	(	PUNCT
njb-39	96	4	cpe	cpe	PROPN
njb-39	96	5	)	)	PUNCT
njb-39	96	6	positive	positive	ADJ
njb-39	96	7	cell	cell	NOUN
njb-39	96	8	culture	culture	NOUN
njb-39	96	9	bottles	bottle	NOUN
njb-39	96	10	for	for	ADP
njb-39	96	11	adenovirus	adenovirus	NOUN
njb-39	96	12	infected	infect	VERB
njb-39	96	13	cells	cell	NOUN
njb-39	96	14	showed	show	VERB
njb-39	96	15	cell	cell	NOUN
njb-39	96	16	grapening	grapening	NOUN
njb-39	96	17	,	,	PUNCT
njb-39	96	18	clustering	clustering	NOUN
njb-39	96	19	,	,	PUNCT
njb-39	96	20	rounding	rounding	NOUN
njb-39	96	21	,	,	PUNCT
njb-39	96	22	clumping	clumping	NOUN
njb-39	96	23	and	and	CCONJ
njb-39	96	24	focal	focal	ADJ
njb-39	96	25	dislodging	dislodging	NOUN
njb-39	96	26	of	of	ADP
njb-39	96	27	cells	cell	NOUN
njb-39	96	28	in	in	ADP
njb-39	96	29	comparison	comparison	NOUN
njb-39	96	30	to	to	PART
njb-39	96	31	control	control	VERB
njb-39	96	32	mock	mock	NOUN
njb-39	96	33	infected	infect	VERB
njb-39	96	34	cells	cell	NOUN
njb-39	96	35	were	be	AUX
njb-39	96	36	seen	see	VERB
njb-39	96	37	on	on	ADP
njb-39	96	38	third	third	ADV
njb-39	96	39	to	to	ADP
njb-39	96	40	fourth	fourth	ADJ
njb-39	96	41	day	day	NOUN
njb-39	96	42	post	post	NOUN
njb-39	96	43	inoculation	inoculation	NOUN
njb-39	96	44	.	.	PUNCT
njb-39	97	1	cpe	cpe	PROPN
njb-39	97	2	positive	positive	ADJ
njb-39	97	3	cells	cell	NOUN
njb-39	97	4	were	be	AUX
njb-39	97	5	scrapped	scrap	VERB
njb-39	97	6	with	with	ADP
njb-39	97	7	a	a	DET
njb-39	97	8	sterile	sterile	ADJ
njb-39	97	9	cell	cell	NOUN
njb-39	97	10	scrapper	scrapper	NOUN
njb-39	97	11	and	and	CCONJ
njb-39	97	12	subjected	subject	VERB
njb-39	97	13	to	to	ADP
njb-39	97	14	immunofluorescence	immunofluorescence	NOUN
njb-39	97	15	using	use	VERB
njb-39	97	16	polyclonal	polyclonal	ADJ
njb-39	97	17	antisera	antisera	NOUN
njb-39	97	18	for	for	ADP
njb-39	97	19	adv	adv	PROPN
njb-39	97	20	.	.	PUNCT
njb-39	98	1	it	it	PRON
njb-39	98	2	showed	show	VERB
njb-39	98	3	the	the	DET
njb-39	98	4	characteristic	characteristic	ADJ
njb-39	98	5	intra	intra	ADJ
njb-39	98	6	nuclear	nuclear	ADJ
njb-39	98	7	brilliant	brilliant	ADJ
njb-39	98	8	apple	apple	NOUN
njb-39	98	9	green	green	ADJ
njb-39	98	10	fluorescence	fluorescence	PROPN
njb-39	98	11	in	in	ADP
njb-39	98	12	adeno	adeno	NOUN
njb-39	98	13	infected	infect	VERB
njb-39	98	14	cells	cell	NOUN
njb-39	98	15	.	.	PUNCT
njb-39	99	1	adenovirus	adenovirus	NOUN
njb-39	99	2	pcr	pcr	PROPN
njb-39	99	3	was	be	AUX
njb-39	99	4	done	do	VERB
njb-39	99	5	in	in	ADP
njb-39	99	6	virus	virus	NOUN
njb-39	99	7	infected	infect	VERB
njb-39	99	8	cell	cell	NOUN
njb-39	99	9	line	line	NOUN
njb-39	99	10	where	where	SCONJ
njb-39	99	11	a	a	DET
njb-39	99	12	fragment	fragment	NOUN
njb-39	99	13	of	of	ADP
njb-39	99	14	161bp	161bp	NOUN
njb-39	99	15	was	be	AUX
njb-39	99	16	amplified	amplify	VERB
njb-39	99	17	from	from	ADP
njb-39	99	18	hexon	hexon	ADJ
njb-39	99	19	gene	gene	NOUN
njb-39	99	20	of	of	ADP
njb-39	99	21	adv	adv	PROPN
njb-39	99	22	genome	genome	PROPN
njb-39	99	23	and	and	CCONJ
njb-39	99	24	visualized	visualize	VERB
njb-39	99	25	by	by	ADP
njb-39	99	26	2	2	NUM
njb-39	99	27	%	%	NOUN
njb-39	99	28	agarose	agarose	NOUN
njb-39	99	29	gel	gel	NOUN
njb-39	99	30	electrophoresis	electrophoresis	NOUN
njb-39	99	31	(	(	PUNCT
njb-39	99	32	figure	figure	NOUN
njb-39	99	33	1	1	NUM
njb-39	99	34	)	)	PUNCT
njb-39	99	35	.	.	PUNCT
njb-39	100	1	figure	figure	NOUN
njb-39	100	2	1	1	NUM
njb-39	100	3	:	:	PUNCT
njb-39	100	4	agarose	agarose	NOUN
njb-39	100	5	gel	gel	NOUN
njb-39	100	6	analysis	analysis	NOUN
njb-39	100	7	of	of	ADP
njb-39	100	8	adenovirus	adenovirus	NOUN
njb-39	100	9	pcr	pcr	PROPN
njb-39	100	10	;	;	PUNCT
njb-39	100	11	lane1	lane1	PROPN
njb-39	100	12	:	:	PUNCT
njb-39	100	13	100bp	100bp	PROPN
njb-39	100	14	molecular	molecular	PROPN
njb-39	100	15	marker	marker	NOUN
njb-39	100	16	(	(	PUNCT
njb-39	100	17	fermentas	fermenta	NOUN
njb-39	100	18	,	,	PUNCT
njb-39	100	19	usa	usa	PROPN
njb-39	100	20	)	)	PUNCT
njb-39	100	21	,	,	PUNCT
njb-39	100	22	lane2	lane2	PROPN
njb-39	100	23	:	:	PUNCT
njb-39	100	24	negative	negative	ADJ
njb-39	100	25	control	control	NOUN
njb-39	100	26	,	,	PUNCT
njb-39	100	27	lane3	lane3	PROPN
njb-39	100	28	:	:	PUNCT
njb-39	100	29	adv	adv	PROPN
njb-39	100	30	these	these	DET
njb-39	100	31	culture	culture	NOUN
njb-39	100	32	bottles	bottle	NOUN
njb-39	100	33	were	be	AUX
njb-39	100	34	preserved	preserve	VERB
njb-39	100	35	at	at	ADP
njb-39	100	36	-80ºc	-80ºc	PROPN
njb-39	100	37	deep	deep	ADJ
njb-39	100	38	freezer	freezer	NOUN
njb-39	100	39	and	and	CCONJ
njb-39	100	40	subjected	subject	VERB
njb-39	100	41	to	to	ADP
njb-39	100	42	repeated	repeat	VERB
njb-39	100	43	freeze	freeze	NOUN
njb-39	100	44	and	and	CCONJ
njb-39	100	45	thaw	thaw	VERB
njb-39	100	46	in	in	ADP
njb-39	100	47	three	three	NUM
njb-39	100	48	occasions	occasion	NOUN
njb-39	100	49	for	for	ADP
njb-39	100	50	cell	cell	NOUN
njb-39	100	51	lysis	lysis	NOUN
njb-39	100	52	.	.	PUNCT
njb-39	101	1	the	the	DET
njb-39	101	2	supernatant	supernatant	NOUN
njb-39	101	3	was	be	AUX
njb-39	101	4	transferred	transfer	VERB
njb-39	101	5	to	to	ADP
njb-39	101	6	a	a	DET
njb-39	101	7	sterile	sterile	ADJ
njb-39	101	8	vial	vial	NOUN
njb-39	101	9	and	and	CCONJ
njb-39	101	10	centrifuged	centrifuge	VERB
njb-39	101	11	aseptically	aseptically	ADV
njb-39	101	12	.	.	PUNCT
njb-39	102	1	the	the	DET
njb-39	102	2	clear	clear	ADJ
njb-39	102	3	supernatant	supernatant	ADJ
njb-39	102	4	occasions	occasion	NOUN
njb-39	102	5	for	for	ADP
njb-39	102	6	cell	cell	NOUN
njb-39	102	7	lysis	lysis	NOUN
njb-39	102	8	.	.	PUNCT
njb-39	103	1	the	the	DET
njb-39	103	2	supernatant	supernatant	NOUN
njb-39	103	3	was	be	AUX
njb-39	103	4	transferred	transfer	VERB
njb-39	103	5	to	to	ADP
njb-39	103	6	a	a	DET
njb-39	103	7	sterile	sterile	ADJ
njb-39	103	8	vial	vial	NOUN
njb-39	103	9	and	and	CCONJ
njb-39	103	10	centrifuged	centrifuge	VERB
njb-39	103	11	aseptically	aseptically	ADV
njb-39	103	12	.	.	PUNCT
njb-39	104	1	the	the	DET
njb-39	104	2	clear	clear	ADJ
njb-39	104	3	supernatant	supernatant	NOUN
njb-39	104	4	containing	contain	VERB
njb-39	104	5	the	the	DET
njb-39	104	6	virus	virus	NOUN
njb-39	104	7	particles	particle	NOUN
njb-39	104	8	was	be	AUX
njb-39	104	9	used	use	VERB
njb-39	104	10	as	as	ADP
njb-39	104	11	positive	positive	ADJ
njb-39	104	12	control	control	NOUN
njb-39	104	13	for	for	ADP
njb-39	104	14	qualitative	qualitative	ADJ
njb-39	104	15	rt	rt	NOUN
njb-39	104	16	-	-	PUNCT
njb-39	104	17	pcr	pcr	PROPN
njb-39	104	18	as	as	ADV
njb-39	104	19	well	well	ADV
njb-39	104	20	as	as	ADP
njb-39	104	21	preparation	preparation	NOUN
njb-39	104	22	of	of	ADP
njb-39	104	23	standards	standard	NOUN
njb-39	104	24	in	in	ADP
njb-39	104	25	quantitative	quantitative	ADJ
njb-39	104	26	real	real	ADJ
njb-39	104	27	time	time	NOUN
njb-39	104	28	rt	rt	PROPN
njb-39	104	29	-	-	PUNCT
njb-39	104	30	pcr	pcr	PROPN
njb-39	104	31	.	.	PUNCT
njb-39	105	1	comparison	comparison	NOUN
njb-39	105	2	of	of	ADP
njb-39	105	3	tcid50	tcid50	ADJ
njb-39	105	4	titer	titer	NOUN
njb-39	105	5	of	of	ADP
njb-39	105	6	lyophilized	lyophilize	VERB
njb-39	105	7	virus	virus	NOUN
njb-39	105	8	stored	store	VERB
njb-39	105	9	for	for	ADP
njb-39	105	10	different	different	ADJ
njb-39	105	11	time	time	NOUN
njb-39	105	12	intervals	interval	NOUN
njb-39	105	13	the	the	DET
njb-39	105	14	lyophilized	lyophilize	VERB
njb-39	105	15	virus	virus	NOUN
njb-39	105	16	was	be	AUX
njb-39	105	17	stored	store	VERB
njb-39	105	18	at	at	ADP
njb-39	105	19	room	room	NOUN
njb-39	105	20	temperature	temperature	NOUN
njb-39	105	21	(	(	PUNCT
njb-39	105	22	rt	rt	PROPN
njb-39	105	23	)	)	PUNCT
njb-39	105	24	and	and	CCONJ
njb-39	105	25	4	4	NUM
njb-39	105	26	°	°	NOUN
njb-39	105	27	c	c	NOUN
njb-39	105	28	for	for	ADP
njb-39	105	29	1month	1month	NUM
njb-39	105	30	,	,	PUNCT
njb-39	105	31	4	4	NUM
njb-39	105	32	months	month	NOUN
njb-39	105	33	and	and	CCONJ
njb-39	105	34	6	6	NUM
njb-39	105	35	months	month	NOUN
njb-39	105	36	.	.	PUNCT
njb-39	106	1	the	the	DET
njb-39	106	2	virus	virus	NOUN
njb-39	106	3	was	be	AUX
njb-39	106	4	revived	revive	VERB
njb-39	106	5	after	after	ADP
njb-39	106	6	these	these	DET
njb-39	106	7	fixed	fix	VERB
njb-39	106	8	intervals	interval	NOUN
njb-39	106	9	of	of	ADP
njb-39	106	10	time	time	NOUN
njb-39	106	11	and	and	CCONJ
njb-39	106	12	infected	infect	VERB
njb-39	106	13	in	in	ADP
njb-39	106	14	hep-2	hep-2	PUNCT
njb-39	106	15	to	to	PART
njb-39	106	16	check	check	VERB
njb-39	106	17	the	the	DET
njb-39	106	18	infectivity	infectivity	NOUN
njb-39	106	19	of	of	ADP
njb-39	106	20	virus	virus	NOUN
njb-39	106	21	and	and	CCONJ
njb-39	106	22	change	change	NOUN
njb-39	106	23	in	in	ADP
njb-39	106	24	the	the	DET
njb-39	106	25	tissue	tissue	NOUN
njb-39	106	26	culture	culture	NOUN
njb-39	106	27	infectious	infectious	ADJ
njb-39	106	28	dose	dose	NOUN
njb-39	106	29	50	50	NUM
njb-39	106	30	(	(	PUNCT
njb-39	106	31	tcid50	tcid50	NOUN
njb-39	106	32	)	)	PUNCT
njb-39	106	33	nepal	nepal	ADJ
njb-39	106	34	journal	journal	NOUN
njb-39	106	35	of	of	ADP
njb-39	106	36	biotechnology	biotechnology	NOUN
njb-39	106	37	.	.	PUNCT
njb-39	107	1	d	d	X
njb-39	107	2	e	e	PROPN
njb-39	107	3	c	c	PROPN
njb-39	107	4	.	.	PUNCT
njb-39	108	1	2	2	NUM
njb-39	108	2	0	0	NUM
njb-39	108	3	1	1	NUM
njb-39	108	4	5	5	NUM
njb-39	108	5	vol	vol	NOUN
njb-39	108	6	.	.	PUNCT
njb-39	109	1	3	3	NUM
njb-39	109	2	,	,	PUNCT
njb-39	109	3	no	no	NOUN
njb-39	109	4	.	.	NOUN
njb-39	109	5	1:15	1:15	NUM
njb-39	109	6	-	-	SYM
njb-39	109	7	21	21	NUM
njb-39	109	8	jha	jha	NOUN
njb-39	109	9	et	et	PROPN
njb-39	109	10	al	al	PROPN
njb-39	109	11	.	.	PUNCT
njb-39	110	1	©	©	PROPN
njb-39	110	2	njb	njb	PROPN
njb-39	110	3	,	,	PUNCT
njb-39	110	4	biotechnology	biotechnology	NOUN
njb-39	110	5	society	society	NOUN
njb-39	110	6	of	of	ADP
njb-39	110	7	nepal	nepal	ADJ
njb-39	110	8	18	18	NUM
njb-39	110	9	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-39	110	10	titer	titer	NOUN
njb-39	110	11	.	.	PUNCT
njb-39	111	1	it	it	PRON
njb-39	111	2	was	be	AUX
njb-39	111	3	observed	observe	VERB
njb-39	111	4	that	that	SCONJ
njb-39	111	5	lyophilized	lyophilize	VERB
njb-39	111	6	virus	virus	NOUN
njb-39	111	7	was	be	AUX
njb-39	111	8	infective	infective	ADJ
njb-39	111	9	till	till	SCONJ
njb-39	111	10	6	6	NUM
njb-39	111	11	months	month	NOUN
njb-39	111	12	and	and	CCONJ
njb-39	111	13	there	there	PRON
njb-39	111	14	was	be	VERB
njb-39	111	15	no	no	DET
njb-39	111	16	change	change	NOUN
njb-39	111	17	in	in	ADP
njb-39	111	18	the	the	DET
njb-39	111	19	tcid50	tcid50	ADJ
njb-39	111	20	titer	titer	NOUN
njb-39	111	21	of	of	ADP
njb-39	111	22	the	the	DET
njb-39	111	23	lyophilized	lyophilize	VERB
njb-39	111	24	adv	adv	PROPN
njb-39	111	25	revived	revive	VERB
njb-39	111	26	after	after	ADP
njb-39	111	27	1month	1month	NUM
njb-39	111	28	to	to	ADP
njb-39	111	29	6	6	NUM
njb-39	111	30	months	month	NOUN
njb-39	111	31	of	of	ADP
njb-39	111	32	storage	storage	NOUN
njb-39	111	33	at	at	ADP
njb-39	111	34	4	4	NUM
njb-39	111	35	°	°	NOUN
njb-39	111	36	c	c	NOUN
njb-39	111	37	.	.	PUNCT
njb-39	112	1	in	in	ADP
njb-39	112	2	case	case	NOUN
njb-39	112	3	of	of	ADP
njb-39	112	4	lyophilized	lyophilize	VERB
njb-39	112	5	adv	adv	PROPN
njb-39	112	6	stored	store	VERB
njb-39	112	7	at	at	ADP
njb-39	112	8	rt	rt	PROPN
njb-39	112	9	the	the	DET
njb-39	112	10	tcid50	tcid50	ADJ
njb-39	112	11	titer	titer	NOUN
njb-39	112	12	was	be	AUX
njb-39	112	13	observed	observe	VERB
njb-39	112	14	to	to	PART
njb-39	112	15	reduce	reduce	VERB
njb-39	112	16	from	from	ADP
njb-39	112	17	10	10	NUM
njb-39	112	18	-	-	SYM
njb-39	112	19	4.5	4.5	NUM
njb-39	112	20	at	at	ADP
njb-39	112	21	1	1	NUM
njb-39	112	22	month	month	NOUN
njb-39	112	23	to	to	ADP
njb-39	112	24	10	10	NUM
njb-39	112	25	-	-	SYM
njb-39	112	26	3.22	3.22	NUM
njb-39	112	27	at	at	ADP
njb-39	112	28	4	4	NUM
njb-39	112	29	months	month	NOUN
njb-39	112	30	but	but	CCONJ
njb-39	112	31	it	it	PRON
njb-39	112	32	remained	remain	VERB
njb-39	112	33	constant	constant	ADJ
njb-39	112	34	to	to	ADP
njb-39	112	35	10	10	NUM
njb-39	112	36	-	-	SYM
njb-39	112	37	3.22	3.22	NUM
njb-39	112	38	after	after	ADP
njb-39	112	39	6	6	NUM
njb-39	112	40	months	month	NOUN
njb-39	112	41	of	of	ADP
njb-39	112	42	storage	storage	NOUN
njb-39	112	43	(	(	PUNCT
njb-39	112	44	figure	figure	NOUN
njb-39	112	45	2	2	NUM
njb-39	112	46	and	and	CCONJ
njb-39	112	47	3	3	NUM
njb-39	112	48	)	)	PUNCT
njb-39	112	49	.	.	PUNCT
njb-39	113	1	figure	figure	NOUN
njb-39	113	2	2	2	NUM
njb-39	113	3	:	:	PUNCT
njb-39	113	4	tcid50	tcid50	NOUN
njb-39	113	5	of	of	ADP
njb-39	113	6	lyophilized	lyophilized	ADJ
njb-39	113	7	virus	virus	NOUN
njb-39	113	8	stored	store	VERB
njb-39	113	9	at	at	ADP
njb-39	113	10	rt	rt	PROPN
njb-39	113	11	and	and	CCONJ
njb-39	113	12	4	4	NUM
njb-39	113	13	°	°	NOUN
njb-39	113	14	c	c	NOUN
njb-39	113	15	and	and	CCONJ
njb-39	113	16	revived	revive	VERB
njb-39	113	17	after	after	ADP
njb-39	113	18	1	1	NUM
njb-39	113	19	month	month	NOUN
njb-39	113	20	,	,	PUNCT
njb-39	113	21	4	4	NUM
njb-39	113	22	and	and	CCONJ
njb-39	113	23	6	6	NUM
njb-39	113	24	months	month	NOUN
njb-39	113	25	.	.	PUNCT
njb-39	114	1	the	the	DET
njb-39	114	2	value	value	NOUN
njb-39	114	3	of	of	ADP
njb-39	114	4	tcid50	tcid50	NOUN
njb-39	114	5	on	on	ADP
njb-39	114	6	yaxis	yaxis	NOUN
njb-39	114	7	corresponds	correspond	VERB
njb-39	114	8	to	to	ADP
njb-39	114	9	10	10	NUM
njb-39	114	10	-	-	PUNCT
njb-39	114	11	y	y	NOUN
njb-39	114	12	of	of	ADP
njb-39	114	13	different	different	ADJ
njb-39	114	14	sample	sample	NOUN
njb-39	114	15	of	of	ADP
njb-39	114	16	adenovirus	adenovirus	NOUN
njb-39	114	17	.	.	PUNCT
njb-39	115	1	figure	figure	NOUN
njb-39	115	2	3	3	NUM
njb-39	115	3	:	:	PUNCT
njb-39	115	4	comparison	comparison	NOUN
njb-39	115	5	of	of	ADP
njb-39	115	6	tcid50	tcid50	NOUN
njb-39	115	7	in	in	ADP
njb-39	115	8	stock	stock	NOUN
njb-39	115	9	virus	virus	NOUN
njb-39	115	10	and	and	CCONJ
njb-39	115	11	lyophilized	lyophilize	VERB
njb-39	115	12	virus	virus	NOUN
njb-39	115	13	revived	revive	VERB
njb-39	115	14	at	at	ADP
njb-39	115	15	different	different	ADJ
njb-39	115	16	time	time	NOUN
njb-39	115	17	intervals	interval	NOUN
njb-39	115	18	.	.	PUNCT
njb-39	116	1	the	the	DET
njb-39	116	2	value	value	NOUN
njb-39	116	3	of	of	ADP
njb-39	116	4	tcid50	tcid50	NOUN
njb-39	116	5	on	on	ADP
njb-39	116	6	yaxis	yaxis	NOUN
njb-39	116	7	corresponds	correspond	VERB
njb-39	116	8	to	to	ADP
njb-39	116	9	10	10	NUM
njb-39	116	10	-	-	PUNCT
njb-39	116	11	y	y	NOUN
njb-39	116	12	of	of	ADP
njb-39	116	13	different	different	ADJ
njb-39	116	14	sample	sample	NOUN
njb-39	116	15	of	of	ADP
njb-39	116	16	adenovirus	adenovirus	NOUN
njb-39	116	17	comparison	comparison	NOUN
njb-39	116	18	of	of	ADP
njb-39	116	19	tcid50	tcid50	ADJ
njb-39	116	20	titer	titer	NOUN
njb-39	116	21	of	of	ADP
njb-39	116	22	lyophilized	lyophilize	VERB
njb-39	116	23	virus	virus	NOUN
njb-39	116	24	stored	store	VERB
njb-39	116	25	for	for	ADP
njb-39	116	26	different	different	ADJ
njb-39	116	27	time	time	NOUN
njb-39	116	28	intervals	interval	NOUN
njb-39	116	29	and	and	CCONJ
njb-39	116	30	stock	stock	NOUN
njb-39	116	31	virus	virus	NOUN
njb-39	116	32	the	the	DET
njb-39	116	33	tcid50	tcid50	ADJ
njb-39	116	34	titer	titer	NOUN
njb-39	116	35	of	of	ADP
njb-39	116	36	the	the	DET
njb-39	116	37	lyophilized	lyophilize	VERB
njb-39	116	38	adenovirus	adenovirus	NOUN
njb-39	116	39	stored	store	VERB
njb-39	116	40	at	at	ADP
njb-39	116	41	rt	rt	PROPN
njb-39	116	42	and	and	CCONJ
njb-39	116	43	4	4	NUM
njb-39	116	44	°	°	NOUN
njb-39	116	45	c	c	NOUN
njb-39	116	46	for	for	ADP
njb-39	116	47	1	1	NUM
njb-39	116	48	,	,	PUNCT
njb-39	116	49	4	4	NUM
njb-39	116	50	and	and	CCONJ
njb-39	116	51	6	6	NUM
njb-39	116	52	months	month	NOUN
njb-39	116	53	was	be	AUX
njb-39	116	54	compared	compare	VERB
njb-39	116	55	with	with	ADP
njb-39	116	56	that	that	PRON
njb-39	116	57	of	of	ADP
njb-39	116	58	the	the	DET
njb-39	116	59	stock	stock	NOUN
njb-39	116	60	virus	virus	NOUN
njb-39	116	61	.	.	PUNCT
njb-39	117	1	the	the	DET
njb-39	117	2	tcid50	tcid50	ADJ
njb-39	117	3	titer	titer	NOUN
njb-39	117	4	of	of	ADP
njb-39	117	5	lyophilized	lyophilize	VERB
njb-39	117	6	virus	virus	NOUN
njb-39	117	7	is	be	AUX
njb-39	117	8	summarized	summarize	VERB
njb-39	117	9	in	in	ADP
njb-39	117	10	table	table	NOUN
njb-39	117	11	1	1	NUM
njb-39	117	12	whereas	whereas	SCONJ
njb-39	117	13	the	the	DET
njb-39	117	14	tcid50	tcid50	ADJ
njb-39	117	15	titer	titer	NOUN
njb-39	117	16	of	of	ADP
njb-39	117	17	stock	stock	NOUN
njb-39	117	18	virus	virus	NOUN
njb-39	117	19	was	be	AUX
njb-39	117	20	observed	observe	VERB
njb-39	117	21	to	to	PART
njb-39	117	22	be	be	AUX
njb-39	117	23	10	10	NUM
njb-39	117	24	-	-	SYM
njb-39	117	25	3	3	NUM
njb-39	117	26	.	.	NOUN
njb-39	117	27	table	table	NOUN
njb-39	117	28	1	1	NUM
njb-39	117	29	:	:	PUNCT
njb-39	117	30	arrangement	arrangement	NOUN
njb-39	117	31	of	of	ADP
njb-39	117	32	data	datum	NOUN
njb-39	117	33	in	in	ADP
njb-39	117	34	calculation	calculation	NOUN
njb-39	117	35	of	of	ADP
njb-39	117	36	tcid50	tcid50	ADJ
njb-39	117	37	titer	titer	NOUN
njb-39	117	38	by	by	ADP
njb-39	117	39	reed	reed	NOUN
njb-39	117	40	and	and	CCONJ
njb-39	117	41	muench	muench	NOUN
njb-39	117	42	formula	formula	NOUN
njb-39	117	43	virus	virus	NOUN
njb-39	117	44	dilution	dilution	NOUN
njb-39	117	45	infected	infect	VERB
njb-39	117	46	noninfected	noninfected	ADJ
njb-39	117	47	accumulative	accumulative	ADJ
njb-39	117	48	values	value	NOUN
njb-39	117	49	infected	infect	VERB
njb-39	117	50	non	non	ADJ
njb-39	117	51	–	–	PUNCT
njb-39	117	52	infected	infected	ADJ
njb-39	117	53	mortality	mortality	NOUN
njb-39	117	54	ratio	ratio	NOUN
njb-39	117	55	percent	percent	NOUN
njb-39	117	56	10	10	NUM
njb-39	117	57	-	-	SYM
njb-39	117	58	1	1	NUM
njb-39	117	59	4	4	NUM
njb-39	117	60	0	0	NUM
njb-39	117	61	14	14	NUM
njb-39	117	62	0	0	NUM
njb-39	117	63	14/14	14/14	NUM
njb-39	117	64	100	100	NUM
njb-39	117	65	10	10	NUM
njb-39	117	66	-	-	SYM
njb-39	117	67	2	2	NUM
njb-39	117	68	4	4	NUM
njb-39	117	69	0	0	NUM
njb-39	117	70	10	10	NUM
njb-39	117	71	0	0	NUM
njb-39	117	72	10/10	10/10	NUM
njb-39	117	73	100	100	NUM
njb-39	117	74	10	10	NUM
njb-39	117	75	-	-	SYM
njb-39	117	76	3	3	NUM
njb-39	117	77	3	3	NUM
njb-39	117	78	1	1	NUM
njb-39	117	79	6	6	NUM
njb-39	117	80	1	1	NUM
njb-39	117	81	6/7	6/7	NUM
njb-39	117	82	85.71	85.71	NUM
njb-39	117	83	10	10	NUM
njb-39	117	84	-	-	SYM
njb-39	117	85	4	4	NUM
njb-39	117	86	2	2	NUM
njb-39	117	87	2	2	NUM
njb-39	117	88	3	3	NUM
njb-39	117	89	3	3	NUM
njb-39	117	90	3/6	3/6	NUM
njb-39	117	91	50	50	NUM
njb-39	117	92	10	10	NUM
njb-39	117	93	-	-	SYM
njb-39	117	94	5	5	NUM
njb-39	117	95	1	1	NUM
njb-39	117	96	3	3	NUM
njb-39	117	97	1	1	NUM
njb-39	117	98	6	6	NUM
njb-39	117	99	1/7	1/7	NUM
njb-39	117	100	14.28	14.28	NUM
njb-39	117	101	10	10	NUM
njb-39	117	102	-	-	SYM
njb-39	117	103	6	6	NUM
njb-39	117	104	0	0	NUM
njb-39	117	105	4	4	NUM
njb-39	117	106	o	o	NOUN
njb-39	117	107	10	10	NUM
njb-39	117	108	0/10	0/10	NUM
njb-39	117	109	0	0	NUM
njb-39	118	1	no	no	DET
njb-39	118	2	significant	significant	ADJ
njb-39	118	3	difference	difference	NOUN
njb-39	118	4	(	(	PUNCT
njb-39	118	5	p	p	NOUN
njb-39	118	6	,	,	PUNCT
njb-39	118	7	0.342	0.342	NUM
njb-39	118	8	)	)	PUNCT
njb-39	118	9	was	be	AUX
njb-39	118	10	observed	observe	VERB
njb-39	118	11	in	in	ADP
njb-39	118	12	the	the	DET
njb-39	118	13	tcid50	tcid50	ADJ
njb-39	118	14	titer	titer	NOUN
njb-39	118	15	or	or	CCONJ
njb-39	118	16	the	the	DET
njb-39	118	17	infectivity	infectivity	NOUN
njb-39	118	18	of	of	ADP
njb-39	118	19	the	the	DET
njb-39	118	20	lyophilized	lyophilize	VERB
njb-39	118	21	virus	virus	NOUN
njb-39	118	22	stored	store	VERB
njb-39	118	23	at	at	ADP
njb-39	118	24	4	4	NUM
njb-39	118	25	°	°	NOUN
njb-39	118	26	c	c	NOUN
njb-39	118	27	for	for	ADP
njb-39	118	28	1	1	NUM
njb-39	118	29	,	,	PUNCT
njb-39	118	30	4	4	NUM
njb-39	118	31	and	and	CCONJ
njb-39	118	32	6	6	NUM
njb-39	118	33	months	month	NOUN
njb-39	118	34	as	as	ADV
njb-39	118	35	well	well	ADV
njb-39	118	36	as	as	ADP
njb-39	118	37	adv	adv	X
njb-39	118	38	stored	store	VERB
njb-39	118	39	at	at	ADP
njb-39	118	40	rt	rt	PROPN
njb-39	118	41	for	for	ADP
njb-39	118	42	4	4	NUM
njb-39	118	43	and	and	CCONJ
njb-39	118	44	6	6	NUM
njb-39	118	45	months	month	NOUN
njb-39	118	46	as	as	ADP
njb-39	118	47	compared	compare	VERB
njb-39	118	48	to	to	ADP
njb-39	118	49	the	the	DET
njb-39	118	50	stock	stock	NOUN
njb-39	118	51	virus	virus	NOUN
njb-39	118	52	.	.	PUNCT
njb-39	119	1	the	the	DET
njb-39	119	2	adenoviral	adenoviral	ADJ
njb-39	119	3	load	load	NOUN
njb-39	119	4	in	in	ADP
njb-39	119	5	stock	stock	NOUN
njb-39	119	6	and	and	CCONJ
njb-39	119	7	lyophilized	lyophilize	VERB
njb-39	119	8	adenovirus	adenovirus	NOUN
njb-39	119	9	was	be	AUX
njb-39	119	10	correlated	correlate	VERB
njb-39	119	11	with	with	ADP
njb-39	119	12	tcid50	tcid50	NOUN
njb-39	119	13	titer	titer	NOUN
njb-39	119	14	as	as	SCONJ
njb-39	119	15	described	describe	VERB
njb-39	119	16	in	in	ADP
njb-39	119	17	the	the	DET
njb-39	119	18	table	table	NOUN
njb-39	119	19	2	2	NUM
njb-39	119	20	using	use	VERB
njb-39	119	21	spearman	spearman	NOUN
njb-39	119	22	correlation	correlation	NOUN
njb-39	119	23	coefficient	coefficient	NOUN
njb-39	119	24	.	.	PUNCT
njb-39	120	1	however	however	ADV
njb-39	120	2	,	,	PUNCT
njb-39	120	3	no	no	DET
njb-39	120	4	statistical	statistical	ADJ
njb-39	120	5	correlation	correlation	NOUN
njb-39	120	6	was	be	AUX
njb-39	120	7	observed	observe	VERB
njb-39	120	8	in	in	ADP
njb-39	120	9	the	the	DET
njb-39	120	10	viral	viral	ADJ
njb-39	120	11	load	load	NOUN
njb-39	120	12	and	and	CCONJ
njb-39	120	13	tcid50	tcid50	ADJ
njb-39	120	14	titer	titer	NOUN
njb-39	120	15	.	.	PUNCT
njb-39	121	1	table	table	NOUN
njb-39	121	2	2	2	NUM
njb-39	121	3	:	:	PUNCT
njb-39	121	4	correlation	correlation	NOUN
njb-39	121	5	of	of	ADP
njb-39	121	6	tcid50	tcid50	NOUN
njb-39	121	7	and	and	CCONJ
njb-39	121	8	viral	viral	ADJ
njb-39	121	9	load	load	NOUN
njb-39	121	10	in	in	ADP
njb-39	121	11	lyophilized	lyophilize	VERB
njb-39	121	12	and	and	CCONJ
njb-39	121	13	stock	stock	NOUN
njb-39	121	14	virus	virus	PROPN
njb-39	121	15	confirmation	confirmation	NOUN
njb-39	121	16	of	of	ADP
njb-39	121	17	successful	successful	ADJ
njb-39	121	18	cloning	cloning	NOUN
njb-39	121	19	hexon	hexon	NOUN
njb-39	121	20	region	region	NOUN
njb-39	121	21	of	of	ADP
njb-39	121	22	the	the	DET
njb-39	121	23	viral	viral	ADJ
njb-39	121	24	genome	genome	NOUN
njb-39	121	25	was	be	AUX
njb-39	121	26	cloned	clone	VERB
njb-39	121	27	in	in	ADP
njb-39	121	28	pgemt	pgemt	NOUN
njb-39	121	29	easy	easy	ADJ
njb-39	121	30	vector	vector	NOUN
njb-39	121	31	(	(	PUNCT
njb-39	121	32	chloramphenicol	chloramphenicol	NOUN
njb-39	121	33	resistance	resistance	NOUN
njb-39	121	34	)	)	PUNCT
njb-39	121	35	and	and	CCONJ
njb-39	121	36	transformed	transform	VERB
njb-39	121	37	in	in	ADP
njb-39	121	38	e	e	PROPN
njb-39	121	39	coli	coli	NOUN
njb-39	121	40	dh5α	dh5α	PROPN
njb-39	121	41	.	.	PROPN
njb-39	121	42	cloned	cloned	ADJ
njb-39	121	43	plasmid	plasmid	NOUN
njb-39	121	44	containing	contain	VERB
njb-39	121	45	the	the	DET
njb-39	121	46	adv	adv	PROPN
njb-39	121	47	hexon	hexon	PROPN
njb-39	121	48	insert	insert	PROPN
njb-39	121	49	had	have	VERB
njb-39	121	50	a	a	DET
njb-39	121	51	defective	defective	ADJ
njb-39	121	52	lacz	lacz	NOUN
njb-39	121	53	gene	gene	NOUN
njb-39	121	54	and	and	CCONJ
njb-39	121	55	those	those	DET
njb-39	121	56	cells	cell	NOUN
njb-39	121	57	are	be	AUX
njb-39	121	58	transformed	transform	VERB
njb-39	121	59	with	with	ADP
njb-39	121	60	this	this	DET
njb-39	121	61	vector	vector	NOUN
njb-39	121	62	formed	form	VERB
njb-39	121	63	white	white	ADJ
njb-39	121	64	colony	colony	NOUN
njb-39	121	65	in	in	ADP
njb-39	121	66	ampicillin	ampicillin	NOUN
njb-39	121	67	agar	agar	NOUN
njb-39	121	68	plate	plate	NOUN
njb-39	121	69	using	use	VERB
njb-39	121	70	jh7	jh7	ADJ
njb-39	121	71	and	and	CCONJ
njb-39	121	72	kw53	kw53	NOUN
njb-39	121	73	primers	primer	NOUN
njb-39	121	74	(	(	PUNCT
njb-39	121	75	figure	figure	NOUN
njb-39	121	76	4	4	NUM
njb-39	121	77	)	)	PUNCT
njb-39	121	78	.	.	PUNCT
njb-39	122	1	figure	figure	NOUN
njb-39	122	2	4	4	NUM
njb-39	122	3	:	:	PUNCT
njb-39	122	4	plate	plate	NOUN
njb-39	122	5	showing	show	VERB
njb-39	122	6	blue	blue	ADJ
njb-39	122	7	(	(	PUNCT
njb-39	122	8	vectors	vector	NOUN
njb-39	122	9	without	without	ADP
njb-39	122	10	inserts	insert	NOUN
njb-39	122	11	)	)	PUNCT
njb-39	122	12	and	and	CCONJ
njb-39	122	13	white	white	ADJ
njb-39	122	14	(	(	PUNCT
njb-39	122	15	vectors	vector	NOUN
njb-39	122	16	with	with	ADP
njb-39	122	17	insert	insert	PROPN
njb-39	122	18	)	)	PUNCT
njb-39	122	19	colonies	colony	NOUN
njb-39	122	20	.	.	PUNCT
njb-39	123	1	stock	stock	NOUN
njb-39	123	2	virus	virus	PROPN
njb-39	123	3	tcid50	tcid50	VERB
njb-39	123	4	viral	viral	ADJ
njb-39	123	5	load	load	NOUN
njb-39	123	6	by	by	ADP
njb-39	123	7	real	real	ADJ
njb-39	123	8	-	-	PUNCT
njb-39	123	9	time	time	NOUN
njb-39	123	10	pcr(copies	pcr(copies	NUM
njb-39	123	11	/	/	SYM
njb-39	123	12	ml	ml	PROPN
njb-39	123	13	)	)	PUNCT
njb-39	123	14	in	in	ADP
njb-39	123	15	104	104	NUM
njb-39	123	16	10	10	NUM
njb-39	123	17	-	-	SYM
njb-39	123	18	3	3	NUM
njb-39	123	19	1590	1590	NUM
njb-39	123	20	store	store	NOUN
njb-39	123	21	d	d	NOUN
njb-39	123	22	at	at	ADP
njb-39	123	23	4	4	NUM
njb-39	123	24	°	°	NOUN
njb-39	123	25	c	c	X
njb-39	123	26	stored	store	VERB
njb-39	123	27	at	at	ADP
njb-39	123	28	rt	rt	PROPN
njb-39	123	29	stored	store	VERB
njb-39	123	30	at	at	ADP
njb-39	123	31	4	4	NUM
njb-39	123	32	°	°	NOUN
njb-39	123	33	c	c	X
njb-39	123	34	stored	store	VERB
njb-39	123	35	at	at	ADP
njb-39	123	36	rt	rt	PROPN
njb-39	123	37	lyophilized	lyophilized	PROPN
njb-39	123	38	adv	adv	PROPN
njb-39	123	39	revived	revive	VERB
njb-39	123	40	after	after	ADP
njb-39	123	41	1	1	NUM
njb-39	123	42	month	month	NOUN
njb-39	123	43	10	10	NUM
njb-39	123	44	-	-	SYM
njb-39	123	45	4.5	4.5	NUM
njb-39	123	46	10	10	NUM
njb-39	123	47	-	-	SYM
njb-39	123	48	3.7	3.7	NUM
njb-39	123	49	2920	2920	NUM
njb-39	123	50	6810	6810	NUM
njb-39	123	51	lyophilized	lyophilize	VERB
njb-39	123	52	adv	adv	PROPN
njb-39	123	53	revived	revive	VERB
njb-39	123	54	after	after	ADP
njb-39	123	55	4	4	NUM
njb-39	123	56	months	month	NOUN
njb-39	123	57	10	10	NUM
njb-39	123	58	-	-	SYM
njb-39	123	59	3.22	3.22	NUM
njb-39	123	60	10	10	NUM
njb-39	123	61	-	-	SYM
njb-39	123	62	4.5	4.5	NUM
njb-39	123	63	2220	2220	NUM
njb-39	123	64	7200	7200	NUM
njb-39	123	65	lyophilized	lyophilize	VERB
njb-39	123	66	adv	adv	PROPN
njb-39	123	67	revived	revive	VERB
njb-39	123	68	after	after	ADP
njb-39	123	69	6	6	NUM
njb-39	123	70	months	month	NOUN
njb-39	123	71	10	10	NUM
njb-39	123	72	-	-	SYM
njb-39	123	73	3.22	3.22	NUM
njb-39	123	74	10	10	NUM
njb-39	123	75	-	-	SYM
njb-39	123	76	3.7	3.7	NUM
njb-39	123	77	672	672	NUM
njb-39	123	78	2650	2650	NUM
njb-39	123	79	nepal	nepal	ADJ
njb-39	123	80	journal	journal	NOUN
njb-39	123	81	of	of	ADP
njb-39	123	82	biotechnology	biotechnology	NOUN
njb-39	123	83	.	.	PUNCT
njb-39	124	1	d	d	X
njb-39	124	2	e	e	PROPN
njb-39	124	3	c	c	PROPN
njb-39	124	4	.	.	PUNCT
njb-39	125	1	2	2	NUM
njb-39	125	2	0	0	NUM
njb-39	125	3	1	1	NUM
njb-39	125	4	5	5	NUM
njb-39	125	5	vol	vol	NOUN
njb-39	125	6	.	.	PUNCT
njb-39	126	1	3	3	NUM
njb-39	126	2	,	,	PUNCT
njb-39	126	3	no	no	NOUN
njb-39	126	4	.	.	NOUN
njb-39	126	5	1:15	1:15	NUM
njb-39	126	6	-	-	SYM
njb-39	126	7	21	21	NUM
njb-39	126	8	jha	jha	NOUN
njb-39	126	9	et	et	PROPN
njb-39	126	10	al	al	PROPN
njb-39	126	11	.	.	PUNCT
njb-39	127	1	©	©	PROPN
njb-39	127	2	njb	njb	PROPN
njb-39	127	3	,	,	PUNCT
njb-39	127	4	biotechnology	biotechnology	NOUN
njb-39	127	5	society	society	NOUN
njb-39	127	6	of	of	ADP
njb-39	127	7	nepal	nepal	ADJ
njb-39	127	8	19	19	NUM
njb-39	127	9	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-39	127	10	white	white	ADJ
njb-39	127	11	colony	colony	NOUN
njb-39	127	12	from	from	ADP
njb-39	127	13	the	the	DET
njb-39	127	14	plate	plate	NOUN
njb-39	127	15	,	,	PUNCT
njb-39	127	16	theoretically	theoretically	ADV
njb-39	127	17	containing	contain	VERB
njb-39	127	18	the	the	DET
njb-39	127	19	desired	desire	VERB
njb-39	127	20	inserts	insert	NOUN
njb-39	127	21	were	be	AUX
njb-39	127	22	picked	pick	VERB
njb-39	127	23	and	and	CCONJ
njb-39	127	24	subjected	subject	VERB
njb-39	127	25	to	to	ADP
njb-39	127	26	plasmid	plasmid	ADJ
njb-39	127	27	extraction	extraction	NOUN
njb-39	127	28	.	.	PUNCT
njb-39	128	1	the	the	DET
njb-39	128	2	plasmids	plasmid	NOUN
njb-39	128	3	isolated	isolate	VERB
njb-39	128	4	from	from	ADP
njb-39	128	5	the	the	DET
njb-39	128	6	white	white	ADJ
njb-39	128	7	colonies	colony	NOUN
njb-39	128	8	were	be	AUX
njb-39	128	9	subjected	subject	VERB
njb-39	128	10	to	to	ADP
njb-39	128	11	pcr	pcr	NOUN
njb-39	128	12	to	to	PART
njb-39	128	13	yield	yield	VERB
njb-39	128	14	161bp	161bp	NOUN
njb-39	128	15	bands	band	NOUN
njb-39	128	16	in	in	ADP
njb-39	128	17	agarose	agarose	NOUN
njb-39	128	18	gel	gel	NOUN
njb-39	128	19	.	.	PUNCT
njb-39	129	1	polymerase	polymerase	NOUN
njb-39	129	2	chain	chain	NOUN
njb-39	129	3	reaction	reaction	NOUN
njb-39	129	4	(	(	PUNCT
njb-39	129	5	pcr	pcr	PROPN
njb-39	129	6	)	)	PUNCT
njb-39	129	7	resulted	result	VERB
njb-39	129	8	in	in	ADP
njb-39	129	9	161	161	NUM
njb-39	129	10	bp	bp	PROPN
njb-39	129	11	amplicon	amplicon	NOUN
njb-39	129	12	with	with	ADP
njb-39	129	13	hexon	hexon	PROPN
njb-39	129	14	region	region	NOUN
njb-39	129	15	specific	specific	PROPN
njb-39	129	16	primer	primer	NOUN
njb-39	129	17	pair	pair	NOUN
njb-39	129	18	(	(	PUNCT
njb-39	129	19	figure	figure	NOUN
njb-39	129	20	5	5	NUM
njb-39	129	21	)	)	PUNCT
njb-39	129	22	.	.	PUNCT
njb-39	130	1	figure	figure	VERB
njb-39	130	2	5	5	NUM
njb-39	130	3	:	:	PUNCT
njb-39	130	4	conformation	conformation	NOUN
njb-39	130	5	of	of	ADP
njb-39	130	6	successful	successful	ADJ
njb-39	130	7	cloning	cloning	NOUN
njb-39	130	8	;	;	PUNCT
njb-39	130	9	lane1	lane1	PROPN
njb-39	130	10	:	:	PUNCT
njb-39	130	11	100	100	NUM
njb-39	130	12	base	base	NOUN
njb-39	130	13	pair	pair	NOUN
njb-39	130	14	molecular	molecular	ADJ
njb-39	130	15	marker	marker	NOUN
njb-39	130	16	(	(	PUNCT
njb-39	130	17	fermentas	fermenta	NOUN
njb-39	130	18	,	,	PUNCT
njb-39	130	19	usa	usa	PROPN
njb-39	130	20	)	)	PUNCT
njb-39	130	21	,	,	PUNCT
njb-39	130	22	lane	lane	NOUN
njb-39	130	23	2	2	NUM
njb-39	130	24	:	:	PUNCT
njb-39	130	25	nc	nc	PROPN
njb-39	130	26	and	and	CCONJ
njb-39	130	27	3	3	NUM
njb-39	130	28	:	:	PUNCT
njb-39	130	29	pc	pc	PROPN
njb-39	130	30	&	&	CCONJ
njb-39	130	31	lane	lane	PROPN
njb-39	130	32	4	4	NUM
njb-39	130	33	:	:	PUNCT
njb-39	130	34	161	161	NUM
njb-39	130	35	bp	bp	PROPN
njb-39	130	36	band	band	NOUN
njb-39	130	37	using	use	VERB
njb-39	130	38	hexon	hexon	ADJ
njb-39	130	39	specific	specific	ADJ
njb-39	130	40	primer	primer	NOUN
njb-39	130	41	in	in	ADP
njb-39	130	42	adv	adv	PROPN
njb-39	130	43	clone	clone	PROPN
njb-39	130	44	.	.	PROPN
njb-39	130	45	comparison	comparison	NOUN
njb-39	130	46	of	of	ADP
njb-39	130	47	viral	viral	ADJ
njb-39	130	48	load	load	NOUN
njb-39	130	49	of	of	ADP
njb-39	130	50	lyophilized	lyophilize	VERB
njb-39	130	51	virus	virus	NOUN
njb-39	130	52	stored	store	VERB
njb-39	130	53	for	for	ADP
njb-39	130	54	different	different	ADJ
njb-39	130	55	time	time	NOUN
njb-39	130	56	intervals	interval	VERB
njb-39	130	57	the	the	DET
njb-39	130	58	effect	effect	NOUN
njb-39	130	59	of	of	ADP
njb-39	130	60	lyophilization	lyophilization	NOUN
njb-39	130	61	on	on	ADP
njb-39	130	62	viral	viral	ADJ
njb-39	130	63	load	load	NOUN
njb-39	130	64	of	of	ADP
njb-39	130	65	adv	adv	PROPN
njb-39	130	66	over	over	ADP
njb-39	130	67	a	a	DET
njb-39	130	68	period	period	NOUN
njb-39	130	69	of	of	ADP
njb-39	130	70	time	time	NOUN
njb-39	130	71	was	be	AUX
njb-39	130	72	studied	study	VERB
njb-39	130	73	.	.	PUNCT
njb-39	131	1	no	no	DET
njb-39	131	2	significant	significant	ADJ
njb-39	131	3	difference	difference	NOUN
njb-39	131	4	was	be	AUX
njb-39	131	5	observed	observe	VERB
njb-39	131	6	in	in	ADP
njb-39	131	7	viral	viral	ADJ
njb-39	131	8	load	load	NOUN
njb-39	131	9	of	of	ADP
njb-39	131	10	adv	adv	X
njb-39	131	11	stored	store	VERB
njb-39	131	12	at	at	ADP
njb-39	131	13	4	4	NUM
njb-39	131	14	°	°	NOUN
njb-39	131	15	c	c	NOUN
njb-39	131	16	for	for	ADP
njb-39	131	17	1	1	NUM
njb-39	131	18	,	,	PUNCT
njb-39	131	19	4	4	NUM
njb-39	131	20	and	and	CCONJ
njb-39	131	21	6	6	NUM
njb-39	131	22	months	month	NOUN
njb-39	131	23	.	.	PUNCT
njb-39	132	1	however	however	ADV
njb-39	132	2	,	,	PUNCT
njb-39	132	3	in	in	ADP
njb-39	132	4	lyophilized	lyophilize	VERB
njb-39	132	5	virus	virus	NOUN
njb-39	132	6	stored	store	VERB
njb-39	132	7	at	at	ADP
njb-39	132	8	rt	rt	PROPN
njb-39	132	9	a	a	DET
njb-39	132	10	significant	significant	ADJ
njb-39	132	11	difference	difference	NOUN
njb-39	132	12	in	in	ADP
njb-39	132	13	viral	viral	ADJ
njb-39	132	14	load	load	NOUN
njb-39	132	15	was	be	AUX
njb-39	132	16	observed	observe	VERB
njb-39	132	17	between	between	ADP
njb-39	132	18	the	the	DET
njb-39	132	19	virus	virus	NOUN
njb-39	132	20	stored	store	VERB
njb-39	132	21	for	for	ADP
njb-39	132	22	1month	1month	NUM
njb-39	132	23	and	and	CCONJ
njb-39	132	24	that	that	PRON
njb-39	132	25	stored	store	VERB
njb-39	132	26	for	for	ADP
njb-39	132	27	6	6	NUM
njb-39	132	28	months	month	NOUN
njb-39	132	29	at	at	ADP
njb-39	132	30	rt	rt	PROPN
njb-39	132	31	(	(	PUNCT
njb-39	132	32	p<0.05	p<0.05	NOUN
njb-39	132	33	)	)	PUNCT
njb-39	132	34	but	but	CCONJ
njb-39	132	35	no	no	DET
njb-39	132	36	difference	difference	NOUN
njb-39	132	37	was	be	AUX
njb-39	132	38	observed	observe	VERB
njb-39	132	39	in	in	ADP
njb-39	132	40	viral	viral	ADJ
njb-39	132	41	load	load	NOUN
njb-39	132	42	of	of	ADP
njb-39	132	43	adv	adv	X
njb-39	132	44	stored	store	VERB
njb-39	132	45	at	at	ADP
njb-39	132	46	rt	rt	PROPN
njb-39	132	47	for	for	ADP
njb-39	132	48	1	1	NUM
njb-39	132	49	and	and	CCONJ
njb-39	132	50	4	4	NUM
njb-39	132	51	months	month	NOUN
njb-39	132	52	(	(	PUNCT
njb-39	132	53	figure	figure	NOUN
njb-39	132	54	6	6	NUM
njb-39	132	55	)	)	PUNCT
njb-39	132	56	.	.	PUNCT
njb-39	133	1	figure	figure	VERB
njb-39	133	2	6	6	NUM
njb-39	133	3	:	:	PUNCT
njb-39	133	4	viral	viral	ADJ
njb-39	133	5	load	load	NOUN
njb-39	133	6	of	of	ADP
njb-39	133	7	lyophilized	lyophilize	VERB
njb-39	133	8	virus	virus	NOUN
njb-39	133	9	stored	store	VERB
njb-39	133	10	at	at	ADP
njb-39	133	11	rt	rt	PROPN
njb-39	133	12	and	and	CCONJ
njb-39	133	13	4	4	NUM
njb-39	133	14	°	°	NOUN
njb-39	133	15	c	c	NOUN
njb-39	133	16	for	for	ADP
njb-39	133	17	1	1	NUM
njb-39	133	18	to	to	PART
njb-39	133	19	6	6	NUM
njb-39	133	20	months	month	NOUN
njb-39	133	21	discussion	discussion	NOUN
njb-39	133	22	freeze	freeze	NOUN
njb-39	133	23	-	-	PUNCT
njb-39	133	24	drying	drying	NOUN
njb-39	133	25	will	will	AUX
njb-39	133	26	not	not	PART
njb-39	133	27	reverse	reverse	VERB
njb-39	133	28	the	the	DET
njb-39	133	29	damage	damage	NOUN
njb-39	133	30	incurred	incur	VERB
njb-39	133	31	prior	prior	ADV
njb-39	133	32	to	to	ADP
njb-39	133	33	formulation	formulation	NOUN
njb-39	133	34	and	and	CCONJ
njb-39	133	35	care	care	NOUN
njb-39	133	36	must	must	AUX
njb-39	133	37	be	be	AUX
njb-39	133	38	exercised	exercise	VERB
njb-39	133	39	when	when	SCONJ
njb-39	133	40	selecting	select	VERB
njb-39	133	41	an	an	DET
njb-39	133	42	appropriate	appropriate	ADJ
njb-39	133	43	cell	cell	NOUN
njb-39	133	44	type	type	NOUN
njb-39	133	45	or	or	CCONJ
njb-39	133	46	technique	technique	NOUN
njb-39	133	47	used	use	VERB
njb-39	133	48	to	to	PART
njb-39	133	49	culture	culture	VERB
njb-39	133	50	or	or	CCONJ
njb-39	133	51	purify	purify	VERB
njb-39	133	52	the	the	DET
njb-39	133	53	cell	cell	NOUN
njb-39	133	54	or	or	CCONJ
njb-39	133	55	its	its	PRON
njb-39	133	56	extracts	extract	NOUN
njb-39	133	57	prior	prior	ADV
njb-39	133	58	to	to	ADP
njb-39	133	59	freezedrying	freezedrye	VERB
njb-39	133	60	.	.	PUNCT
njb-39	134	1	to	to	PART
njb-39	134	2	sustain	sustain	VERB
njb-39	134	3	freeze	freeze	NOUN
njb-39	134	4	-	-	PUNCT
njb-39	134	5	drying	dry	VERB
njb-39	134	6	it	it	PRON
njb-39	134	7	is	be	AUX
njb-39	134	8	necessary	necessary	ADJ
njb-39	134	9	to	to	PART
njb-39	134	10	establish	establish	VERB
njb-39	134	11	a	a	DET
njb-39	134	12	pressure	pressure	NOUN
njb-39	134	13	gradient	gradient	ADJ
njb-39	134	14	from	from	ADP
njb-39	134	15	a	a	DET
njb-39	134	16	sample	sample	NOUN
njb-39	134	17	(	(	PUNCT
njb-39	134	18	highest	high	ADJ
njb-39	134	19	pressure	pressure	NOUN
njb-39	134	20	)	)	PUNCT
njb-39	134	21	,	,	PUNCT
njb-39	134	22	to	to	ADP
njb-39	134	23	condenser	condenser	NOUN
njb-39	134	24	,	,	PUNCT
njb-39	134	25	and	and	CCONJ
njb-39	134	26	finally	finally	ADV
njb-39	134	27	vacuum	vacuum	NOUN
njb-39	134	28	pump	pump	NOUN
njb-39	134	29	(	(	PUNCT
njb-39	134	30	lowest	low	ADJ
njb-39	134	31	pressure	pressure	NOUN
njb-39	134	32	)	)	PUNCT
njb-39	134	33	so	so	SCONJ
njb-39	134	34	that	that	SCONJ
njb-39	134	35	water	water	NOUN
njb-39	134	36	migrates	migrate	VERB
njb-39	134	37	from	from	ADP
njb-39	134	38	the	the	DET
njb-39	134	39	sample	sample	NOUN
njb-39	134	40	as	as	ADP
njb-39	134	41	drying	dry	VERB
njb-39	134	42	progresses	progress	NOUN
njb-39	134	43	.	.	PUNCT
njb-39	135	1	in	in	ADP
njb-39	135	2	the	the	DET
njb-39	135	3	present	present	ADJ
njb-39	135	4	study	study	NOUN
njb-39	135	5	adenovirus	adenovirus	NOUN
njb-39	135	6	was	be	AUX
njb-39	135	7	successfully	successfully	ADV
njb-39	135	8	propagated	propagate	VERB
njb-39	135	9	in	in	ADP
njb-39	135	10	hep-2	hep-2	PUNCT
njb-39	135	11	cells	cell	NOUN
njb-39	135	12	.	.	PUNCT
njb-39	136	1	the	the	DET
njb-39	136	2	lyophilization	lyophilization	NOUN
njb-39	136	3	cycle	cycle	NOUN
njb-39	136	4	was	be	AUX
njb-39	136	5	standardized	standardize	VERB
njb-39	136	6	and	and	CCONJ
njb-39	136	7	the	the	DET
njb-39	136	8	virus	virus	NOUN
njb-39	136	9	suspension	suspension	NOUN
njb-39	136	10	was	be	AUX
njb-39	136	11	lyophilized	lyophilize	VERB
njb-39	136	12	at	at	ADP
njb-39	136	13	temperature	temperature	NOUN
njb-39	136	14	below	below	ADP
njb-39	136	15	-40	-40	NUM
njb-39	136	16	°	°	NOUN
njb-39	136	17	c	c	NOUN
njb-39	136	18	and	and	CCONJ
njb-39	136	19	under	under	ADP
njb-39	136	20	50	50	NUM
njb-39	136	21	m	m	NOUN
njb-39	136	22	torr	torr	NOUN
njb-39	136	23	vacuum	vacuum	NOUN
njb-39	136	24	.	.	PUNCT
njb-39	137	1	the	the	DET
njb-39	137	2	essence	essence	NOUN
njb-39	137	3	of	of	ADP
njb-39	137	4	the	the	DET
njb-39	137	5	formulation	formulation	NOUN
njb-39	137	6	exercise	exercise	NOUN
njb-39	137	7	should	should	AUX
njb-39	137	8	be	be	AUX
njb-39	137	9	to	to	PART
njb-39	137	10	minimize	minimize	VERB
njb-39	137	11	freezedrying	freezedrying	NOUN
njb-39	137	12	damage	damage	NOUN
njb-39	137	13	,	,	PUNCT
njb-39	137	14	loss	loss	NOUN
njb-39	137	15	of	of	ADP
njb-39	137	16	viability	viability	NOUN
njb-39	137	17	,	,	PUNCT
njb-39	137	18	or	or	CCONJ
njb-39	137	19	activity	activity	NOUN
njb-39	137	20	.	.	PUNCT
njb-39	138	1	this	this	DET
njb-39	138	2	study	study	NOUN
njb-39	138	3	considers	consider	VERB
njb-39	138	4	the	the	DET
njb-39	138	5	stability	stability	NOUN
njb-39	138	6	of	of	ADP
njb-39	138	7	adenovirus	adenovirus	NOUN
njb-39	138	8	after	after	ADP
njb-39	138	9	freeze	freeze	NOUN
njb-39	138	10	-	-	PUNCT
njb-39	138	11	drying	drying	NOUN
njb-39	138	12	and	and	CCONJ
njb-39	138	13	storage	storage	NOUN
njb-39	138	14	and	and	CCONJ
njb-39	138	15	also	also	ADV
njb-39	138	16	determines	determine	VERB
njb-39	138	17	its	its	PRON
njb-39	138	18	titer	titer	NOUN
njb-39	138	19	and	and	CCONJ
njb-39	138	20	load	load	NOUN
njb-39	138	21	.	.	PUNCT
njb-39	139	1	the	the	DET
njb-39	139	2	infectivity	infectivity	NOUN
njb-39	139	3	of	of	ADP
njb-39	139	4	the	the	DET
njb-39	139	5	virus	virus	NOUN
njb-39	139	6	was	be	AUX
njb-39	139	7	retained	retain	VERB
njb-39	139	8	when	when	SCONJ
njb-39	139	9	stored	store	VERB
njb-39	139	10	for	for	ADP
njb-39	139	11	over	over	ADP
njb-39	139	12	6	6	NUM
njb-39	139	13	months	month	NOUN
njb-39	139	14	at	at	ADP
njb-39	139	15	4	4	NUM
njb-39	139	16	°	°	NOUN
njb-39	139	17	c	c	NOUN
njb-39	139	18	or	or	CCONJ
njb-39	139	19	rt	rt	PROPN
njb-39	139	20	.	.	PUNCT
njb-39	140	1	we	we	PRON
njb-39	140	2	determined	determine	VERB
njb-39	140	3	the	the	DET
njb-39	140	4	tcid50	tcid50	ADJ
njb-39	140	5	titer	titer	NOUN
njb-39	140	6	of	of	ADP
njb-39	140	7	the	the	DET
njb-39	140	8	adenovirus	adenovirus	NOUN
njb-39	140	9	lyophilized	lyophilize	VERB
njb-39	140	10	for	for	ADP
njb-39	140	11	1	1	NUM
njb-39	140	12	-	-	SYM
njb-39	140	13	6	6	NUM
njb-39	140	14	months	month	NOUN
njb-39	140	15	and	and	CCONJ
njb-39	140	16	observed	observe	VERB
njb-39	140	17	no	no	DET
njb-39	140	18	significant	significant	ADJ
njb-39	140	19	difference	difference	NOUN
njb-39	140	20	in	in	ADP
njb-39	140	21	the	the	DET
njb-39	140	22	infectivity	infectivity	NOUN
njb-39	140	23	when	when	SCONJ
njb-39	140	24	stored	store	VERB
njb-39	140	25	at	at	ADP
njb-39	140	26	4	4	NUM
njb-39	140	27	°	°	NOUN
njb-39	140	28	c	c	NOUN
njb-39	140	29	or	or	CCONJ
njb-39	140	30	rt	rt	PROPN
njb-39	140	31	.	.	PROPN
njb-39	141	1	thus	thus	ADV
njb-39	141	2	,	,	PUNCT
njb-39	141	3	the	the	DET
njb-39	141	4	cell	cell	NOUN
njb-39	141	5	culture	culture	NOUN
njb-39	141	6	-	-	PUNCT
njb-39	141	7	grown	grow	VERB
njb-39	141	8	adenovirus	adenovirus	NOUN
njb-39	141	9	could	could	AUX
njb-39	141	10	withstand	withstand	VERB
njb-39	141	11	the	the	DET
njb-39	141	12	freeze	freeze	NOUN
njb-39	141	13	-	-	PUNCT
njb-39	141	14	drying	dry	VERB
njb-39	141	15	process	process	NOUN
njb-39	141	16	,	,	PUNCT
njb-39	141	17	and	and	CCONJ
njb-39	141	18	it	it	PRON
njb-39	141	19	should	should	AUX
njb-39	141	20	be	be	AUX
njb-39	141	21	stored	store	VERB
njb-39	141	22	at	at	ADP
njb-39	141	23	4	4	NUM
njb-39	141	24	°	°	NOUN
njb-39	141	25	c	c	PROPN
njb-39	141	26	and	and	CCONJ
njb-39	141	27	rt	rt	PROPN
njb-39	141	28	to	to	PART
njb-39	141	29	retain	retain	VERB
njb-39	141	30	maximum	maximum	ADJ
njb-39	141	31	infectivity	infectivity	NOUN
njb-39	141	32	.	.	PUNCT
njb-39	142	1	many	many	ADJ
njb-39	142	2	culture	culture	NOUN
njb-39	142	3	collections	collection	NOUN
njb-39	142	4	and	and	CCONJ
njb-39	142	5	gene	gene	NOUN
njb-39	142	6	banks	bank	NOUN
njb-39	142	7	insist	insist	VERB
njb-39	142	8	on	on	ADP
njb-39	142	9	high	high	ADJ
njb-39	142	10	recovery	recovery	NOUN
njb-39	142	11	values	value	NOUN
njb-39	142	12	prior	prior	ADV
njb-39	142	13	to	to	ADP
njb-39	142	14	a	a	DET
njb-39	142	15	protocol	protocol	NOUN
njb-39	142	16	being	be	AUX
njb-39	142	17	adopted	adopt	VERB
njb-39	142	18	for	for	ADP
njb-39	142	19	regular	regular	ADJ
njb-39	142	20	use	use	NOUN
njb-39	142	21	;	;	PUNCT
njb-39	142	22	50	50	NUM
njb-39	142	23	%	%	NOUN
njb-39	142	24	viability	viability	NOUN
njb-39	142	25	post	post	NOUN
njb-39	142	26	thaw	thaw	PROPN
njb-39	142	27	has	have	AUX
njb-39	142	28	been	be	AUX
njb-39	142	29	accepted	accept	VERB
njb-39	142	30	in	in	ADP
njb-39	142	31	some	some	DET
njb-39	142	32	culture	culture	NOUN
njb-39	142	33	collections	collection	NOUN
njb-39	142	34	as	as	ADP
njb-39	142	35	a	a	DET
njb-39	142	36	nominal	nominal	ADJ
njb-39	142	37	cutoff	cutoff	NOUN
njb-39	142	38	for	for	ADP
njb-39	142	39	adopting	adopt	VERB
njb-39	142	40	maintenance	maintenance	NOUN
njb-39	142	41	by	by	ADP
njb-39	142	42	cryopreservation	cryopreservation	NOUN
njb-39	142	43	alone	alone	ADV
njb-39	142	44	(	(	PUNCT
njb-39	142	45	mcclure	mcclure	VERB
njb-39	142	46	et	et	X
njb-39	142	47	al	al	PROPN
njb-39	142	48	.	.	PROPN
njb-39	142	49	,	,	PUNCT
njb-39	142	50	2011	2011	NUM
njb-39	142	51	)	)	PUNCT
njb-39	142	52	.	.	PUNCT
njb-39	143	1	the	the	DET
njb-39	143	2	ability	ability	NOUN
njb-39	143	3	to	to	PART
njb-39	143	4	determine	determine	VERB
njb-39	143	5	viral	viral	ADJ
njb-39	143	6	titer	titer	NOUN
njb-39	143	7	rapidly	rapidly	ADV
njb-39	143	8	and	and	CCONJ
njb-39	143	9	at	at	ADP
njb-39	143	10	high	high	ADJ
njb-39	143	11	accuracy	accuracy	NOUN
njb-39	143	12	is	be	AUX
njb-39	143	13	one	one	NUM
njb-39	143	14	of	of	ADP
njb-39	143	15	the	the	DET
njb-39	143	16	most	most	ADV
njb-39	143	17	important	important	ADJ
njb-39	143	18	tools	tool	NOUN
njb-39	143	19	desired	desire	VERB
njb-39	143	20	when	when	SCONJ
njb-39	143	21	working	work	VERB
njb-39	143	22	with	with	ADP
njb-39	143	23	viruses	virus	NOUN
njb-39	143	24	in	in	ADP
njb-39	143	25	the	the	DET
njb-39	143	26	research	research	NOUN
njb-39	143	27	laboratory	laboratory	NOUN
njb-39	143	28	[	[	PUNCT
njb-39	143	29	7	7	NUM
njb-39	143	30	,	,	PUNCT
njb-39	143	31	8	8	NUM
njb-39	143	32	]	]	PUNCT
njb-39	143	33	.	.	PUNCT
njb-39	144	1	real	real	ADJ
njb-39	144	2	-	-	PUNCT
njb-39	144	3	time	time	NOUN
njb-39	144	4	pcr	pcr	NOUN
njb-39	144	5	has	have	AUX
njb-39	144	6	been	be	AUX
njb-39	144	7	shown	show	VERB
njb-39	144	8	to	to	PART
njb-39	144	9	be	be	AUX
njb-39	144	10	more	more	ADV
njb-39	144	11	sensitive	sensitive	ADJ
njb-39	144	12	than	than	ADP
njb-39	144	13	cell	cell	NOUN
njb-39	144	14	culture	culture	NOUN
njb-39	144	15	based	base	VERB
njb-39	144	16	techniques	technique	NOUN
njb-39	144	17	for	for	ADP
njb-39	144	18	detection	detection	NOUN
njb-39	144	19	of	of	ADP
njb-39	144	20	viruses	virus	NOUN
njb-39	144	21	in	in	ADP
njb-39	144	22	clinical	clinical	ADJ
njb-39	144	23	specimens	specimen	NOUN
njb-39	144	24	;	;	PUNCT
njb-39	144	25	it	it	PRON
njb-39	144	26	was	be	AUX
njb-39	144	27	therefore	therefore	ADV
njb-39	144	28	interesting	interesting	ADJ
njb-39	144	29	to	to	PART
njb-39	144	30	investigate	investigate	VERB
njb-39	144	31	whether	whether	SCONJ
njb-39	144	32	real	real	ADJ
njb-39	144	33	-	-	PUNCT
njb-39	144	34	time	time	NOUN
njb-39	144	35	pcr	pcr	NOUN
njb-39	144	36	technology	technology	NOUN
njb-39	144	37	could	could	AUX
njb-39	144	38	also	also	ADV
njb-39	144	39	be	be	AUX
njb-39	144	40	an	an	DET
njb-39	144	41	important	important	ADJ
njb-39	144	42	tool	tool	NOUN
njb-39	144	43	for	for	ADP
njb-39	144	44	rapid	rapid	ADJ
njb-39	144	45	and	and	CCONJ
njb-39	144	46	efficient	efficient	ADJ
njb-39	144	47	estimation	estimation	NOUN
njb-39	144	48	of	of	ADP
njb-39	144	49	viral	viral	ADJ
njb-39	144	50	titer	titer	NOUN
njb-39	144	51	[	[	X
njb-39	144	52	9	9	NUM
njb-39	144	53	]	]	PUNCT
njb-39	144	54	.	.	PUNCT
njb-39	145	1	in	in	ADP
njb-39	145	2	the	the	DET
njb-39	145	3	present	present	ADJ
njb-39	145	4	study	study	NOUN
njb-39	145	5	the	the	DET
njb-39	145	6	viral	viral	ADJ
njb-39	145	7	load	load	NOUN
njb-39	145	8	of	of	ADP
njb-39	145	9	stock	stock	NOUN
njb-39	145	10	and	and	CCONJ
njb-39	145	11	lyophilized	lyophilize	VERB
njb-39	145	12	adenovirus	adenovirus	NOUN
njb-39	145	13	was	be	AUX
njb-39	145	14	determined	determine	VERB
njb-39	145	15	and	and	CCONJ
njb-39	145	16	compared	compare	VERB
njb-39	145	17	with	with	ADP
njb-39	145	18	each	each	DET
njb-39	145	19	other	other	ADJ
njb-39	145	20	.	.	PUNCT
njb-39	146	1	the	the	DET
njb-39	146	2	viral	viral	ADJ
njb-39	146	3	load	load	NOUN
njb-39	146	4	of	of	ADP
njb-39	146	5	the	the	DET
njb-39	146	6	lyophilized	lyophilize	VERB
njb-39	146	7	virus	virus	NOUN
njb-39	146	8	stored	store	VERB
njb-39	146	9	at	at	ADP
njb-39	146	10	4	4	NUM
njb-39	146	11	°	°	NOUN
njb-39	146	12	c	c	PROPN
njb-39	146	13	was	be	AUX
njb-39	146	14	observed	observe	VERB
njb-39	146	15	to	to	PART
njb-39	146	16	increase	increase	VERB
njb-39	146	17	as	as	ADP
njb-39	146	18	compared	compare	VERB
njb-39	146	19	to	to	ADP
njb-39	146	20	the	the	DET
njb-39	146	21	stock	stock	NOUN
njb-39	146	22	virus	virus	NOUN
njb-39	146	23	whereas	whereas	SCONJ
njb-39	146	24	that	that	PRON
njb-39	146	25	stored	store	VERB
njb-39	146	26	at	at	ADP
njb-39	146	27	rt	rt	PROPN
njb-39	146	28	for	for	ADP
njb-39	146	29	1	1	NUM
njb-39	146	30	and	and	CCONJ
njb-39	146	31	4	4	NUM
njb-39	146	32	months	month	NOUN
njb-39	146	33	the	the	DET
njb-39	146	34	viral	viral	ADJ
njb-39	146	35	load	load	NOUN
njb-39	146	36	increased	increase	VERB
njb-39	146	37	slightly	slightly	ADV
njb-39	146	38	but	but	CCONJ
njb-39	146	39	at	at	ADP
njb-39	146	40	6	6	NUM
njb-39	146	41	months	month	NOUN
njb-39	146	42	the	the	DET
njb-39	146	43	viral	viral	ADJ
njb-39	146	44	load	load	NOUN
njb-39	146	45	decreased	decrease	VERB
njb-39	146	46	as	as	ADP
njb-39	146	47	compared	compare	VERB
njb-39	146	48	to	to	ADP
njb-39	146	49	the	the	DET
njb-39	146	50	stock	stock	NOUN
njb-39	146	51	virus	virus	NOUN
njb-39	146	52	.	.	PUNCT
njb-39	147	1	there	there	PRON
njb-39	147	2	is	be	VERB
njb-39	147	3	evidence	evidence	NOUN
njb-39	147	4	that	that	SCONJ
njb-39	147	5	a	a	DET
njb-39	147	6	cryopreservation	cryopreservation	NOUN
njb-39	147	7	method	method	NOUN
njb-39	147	8	yielding	yield	VERB
njb-39	147	9	high	high	ADJ
njb-39	147	10	initial	initial	ADJ
njb-39	147	11	recovery	recovery	NOUN
njb-39	147	12	values	value	NOUN
njb-39	147	13	,	,	PUNCT
njb-39	147	14	maintains	maintain	VERB
njb-39	147	15	viability	viability	NOUN
njb-39	147	16	at	at	ADP
njb-39	147	17	that	that	DET
njb-39	147	18	level	level	NOUN
njb-39	147	19	on	on	ADP
njb-39	147	20	prolonged	prolonged	ADJ
njb-39	147	21	storage	storage	NOUN
njb-39	147	22	.	.	PUNCT
njb-39	148	1	titer	titer	NOUN
njb-39	148	2	determined	determine	VERB
njb-39	148	3	using	use	VERB
njb-39	148	4	either	either	CCONJ
njb-39	148	5	by	by	ADP
njb-39	148	6	plaque	plaque	NOUN
njb-39	148	7	forming	form	VERB
njb-39	148	8	unit	unit	NOUN
njb-39	148	9	(	(	PUNCT
njb-39	148	10	pfu	pfu	PROPN
njb-39	148	11	)	)	PUNCT
njb-39	148	12	or	or	CCONJ
njb-39	148	13	endpoint	endpoint	NOUN
njb-39	148	14	dilution	dilution	NOUN
njb-39	148	15	(	(	PUNCT
njb-39	148	16	tcid50	tcid50	INTJ
njb-39	148	17	)	)	PUNCT
njb-39	148	18	assays	assay	NOUN
njb-39	148	19	,	,	PUNCT
njb-39	148	20	methods	method	NOUN
njb-39	148	21	that	that	PRON
njb-39	148	22	are	be	AUX
njb-39	148	23	time	time	NOUN
njb-39	148	24	-	-	PUNCT
njb-39	148	25	consuming	consume	VERB
njb-39	148	26	and	and	CCONJ
njb-39	148	27	labor	labor	NOUN
njb-39	148	28	intensive	intensive	ADJ
njb-39	148	29	[	[	ADP
njb-39	148	30	10	10	NUM
njb-39	148	31	]	]	PUNCT
njb-39	148	32	.	.	PUNCT
njb-39	149	1	as	as	ADP
njb-39	149	2	a	a	DET
njb-39	149	3	complement	complement	NOUN
njb-39	149	4	to	to	ADP
njb-39	149	5	these	these	DET
njb-39	149	6	nepal	nepal	ADJ
njb-39	149	7	journal	journal	NOUN
njb-39	149	8	of	of	ADP
njb-39	149	9	biotechnology	biotechnology	NOUN
njb-39	149	10	.	.	PUNCT
njb-39	150	1	d	d	X
njb-39	150	2	e	e	PROPN
njb-39	150	3	c	c	PROPN
njb-39	150	4	.	.	PUNCT
njb-39	151	1	2	2	NUM
njb-39	151	2	0	0	NUM
njb-39	151	3	1	1	NUM
njb-39	151	4	5	5	NUM
njb-39	151	5	vol	vol	NOUN
njb-39	151	6	.	.	PUNCT
njb-39	152	1	3	3	NUM
njb-39	152	2	,	,	PUNCT
njb-39	152	3	no	no	NOUN
njb-39	152	4	.	.	NOUN
njb-39	152	5	1:15	1:15	NUM
njb-39	152	6	-	-	SYM
njb-39	152	7	21	21	NUM
njb-39	152	8	jha	jha	NOUN
njb-39	152	9	et	et	PROPN
njb-39	152	10	al	al	PROPN
njb-39	152	11	.	.	PUNCT
njb-39	153	1	©	©	PROPN
njb-39	153	2	njb	njb	PROPN
njb-39	153	3	,	,	PUNCT
njb-39	153	4	biotechnology	biotechnology	NOUN
njb-39	153	5	society	society	NOUN
njb-39	153	6	of	of	ADP
njb-39	153	7	nepal	nepal	ADJ
njb-39	153	8	20	20	NUM
njb-39	153	9	nepjol.info/index.php/njb	nepjol.info/index.php/njb	ADJ
njb-39	153	10	established	establish	VERB
njb-39	153	11	methods	method	NOUN
njb-39	153	12	of	of	ADP
njb-39	153	13	determination	determination	NOUN
njb-39	153	14	of	of	ADP
njb-39	153	15	virus	virus	NOUN
njb-39	153	16	titers	titer	NOUN
njb-39	153	17	,	,	PUNCT
njb-39	153	18	we	we	PRON
njb-39	153	19	here	here	ADV
njb-39	153	20	describe	describe	VERB
njb-39	153	21	a	a	DET
njb-39	153	22	real	real	ADJ
njb-39	153	23	-	-	PUNCT
njb-39	153	24	time	time	NOUN
njb-39	153	25	quantitative	quantitative	ADJ
njb-39	153	26	pcr	pcr	NOUN
njb-39	153	27	assay	assay	NOUN
njb-39	153	28	that	that	PRON
njb-39	153	29	enables	enable	VERB
njb-39	153	30	rapid	rapid	ADJ
njb-39	153	31	recording	recording	NOUN
njb-39	153	32	of	of	ADP
njb-39	153	33	viral	viral	ADJ
njb-39	153	34	load	load	NOUN
njb-39	153	35	values	value	NOUN
njb-39	153	36	that	that	PRON
njb-39	153	37	corresponds	correspond	VERB
njb-39	153	38	to	to	ADP
njb-39	153	39	titer	titer	NOUN
njb-39	153	40	measurements	measurement	NOUN
njb-39	153	41	of	of	ADP
njb-39	153	42	viruses	virus	NOUN
njb-39	153	43	.	.	PUNCT
njb-39	154	1	however	however	ADV
njb-39	154	2	,	,	PUNCT
njb-39	154	3	the	the	DET
njb-39	154	4	results	result	NOUN
njb-39	154	5	presented	present	VERB
njb-39	154	6	here	here	ADV
njb-39	154	7	showed	show	VERB
njb-39	154	8	no	no	DET
njb-39	154	9	significant	significant	ADJ
njb-39	154	10	relationship	relationship	NOUN
njb-39	154	11	between	between	ADP
njb-39	154	12	real	real	ADJ
njb-39	154	13	time	time	NOUN
njb-39	154	14	pcr	pcr	ADP
njb-39	154	15	v	v	PROPN
njb-39	154	16	/	/	SYM
njb-39	154	17	s	s	PART
njb-39	154	18	tcid50	tcid50	NOUN
njb-39	154	19	.	.	PUNCT
njb-39	155	1	even	even	ADV
njb-39	155	2	though	though	SCONJ
njb-39	155	3	the	the	DET
njb-39	155	4	tcid50	tcid50	NOUN
njb-39	155	5	technique	technique	NOUN
njb-39	155	6	is	be	AUX
njb-39	155	7	time	time	NOUN
njb-39	155	8	consuming	consume	VERB
njb-39	155	9	,	,	PUNCT
njb-39	155	10	need	need	VERB
njb-39	155	11	expertise	expertise	NOUN
njb-39	155	12	but	but	CCONJ
njb-39	155	13	is	be	AUX
njb-39	155	14	capable	capable	ADJ
njb-39	155	15	of	of	ADP
njb-39	155	16	dealing	deal	VERB
njb-39	155	17	with	with	ADP
njb-39	155	18	the	the	DET
njb-39	155	19	infective	infective	ADJ
njb-39	155	20	/	/	SYM
njb-39	155	21	live	live	NOUN
njb-39	155	22	virus	virus	NOUN
njb-39	155	23	which	which	PRON
njb-39	155	24	has	have	VERB
njb-39	155	25	the	the	DET
njb-39	155	26	great	great	ADJ
njb-39	155	27	importance	importance	NOUN
njb-39	155	28	in	in	ADP
njb-39	155	29	viral	viral	ADJ
njb-39	155	30	isolation	isolation	NOUN
njb-39	155	31	,	,	PUNCT
njb-39	155	32	manufacturing	manufacture	VERB
njb-39	155	33	liveattenuated	liveattenuate	VERB
njb-39	155	34	vaccines	vaccine	NOUN
njb-39	155	35	,	,	PUNCT
njb-39	155	36	viral	viral	ADJ
njb-39	155	37	antigen	antigen	NOUN
njb-39	155	38	and	and	CCONJ
njb-39	155	39	viral	viral	ADJ
njb-39	155	40	standards	standard	NOUN
njb-39	155	41	.	.	PUNCT
njb-39	156	1	real	real	ADJ
njb-39	156	2	time	time	NOUN
njb-39	156	3	pcr	pcr	X
njb-39	156	4	which	which	PRON
njb-39	156	5	detects	detect	VERB
njb-39	156	6	both	both	CCONJ
njb-39	156	7	the	the	DET
njb-39	156	8	infective	infective	ADJ
njb-39	156	9	and	and	CCONJ
njb-39	156	10	non	non	ADJ
njb-39	156	11	-	-	ADJ
njb-39	156	12	infective	infective	ADJ
njb-39	156	13	viral	viral	ADJ
njb-39	156	14	particles	particle	NOUN
njb-39	156	15	is	be	AUX
njb-39	156	16	highly	highly	ADV
njb-39	156	17	sensitive	sensitive	ADJ
njb-39	156	18	and	and	CCONJ
njb-39	156	19	rapid	rapid	ADJ
njb-39	156	20	,	,	PUNCT
njb-39	156	21	used	use	VERB
njb-39	156	22	most	most	ADV
njb-39	156	23	popularly	popularly	ADV
njb-39	156	24	in	in	ADP
njb-39	156	25	the	the	DET
njb-39	156	26	viral	viral	ADJ
njb-39	156	27	diagnostic	diagnostic	ADJ
njb-39	156	28	labs	lab	NOUN
njb-39	156	29	and	and	CCONJ
njb-39	156	30	has	have	VERB
njb-39	156	31	great	great	ADJ
njb-39	156	32	importance	importance	NOUN
njb-39	156	33	in	in	ADP
njb-39	156	34	the	the	DET
njb-39	156	35	early	early	ADJ
njb-39	156	36	diagnosis	diagnosis	NOUN
njb-39	156	37	and	and	CCONJ
njb-39	156	38	management	management	NOUN
njb-39	156	39	by	by	ADP
njb-39	156	40	of	of	ADP
njb-39	156	41	the	the	DET
njb-39	156	42	patient	patient	NOUN
njb-39	156	43	[	[	X
njb-39	156	44	9	9	NUM
njb-39	156	45	]	]	PUNCT
njb-39	156	46	.	.	PUNCT
njb-39	157	1	it	it	PRON
njb-39	157	2	should	should	AUX
njb-39	157	3	,	,	PUNCT
njb-39	157	4	however	however	ADV
njb-39	157	5	,	,	PUNCT
njb-39	157	6	be	be	AUX
njb-39	157	7	taken	take	VERB
njb-39	157	8	into	into	ADP
njb-39	157	9	consideration	consideration	NOUN
njb-39	157	10	that	that	PRON
njb-39	157	11	viruses	viruse	VERB
njb-39	157	12	causing	cause	VERB
njb-39	157	13	persistent	persistent	ADJ
njb-39	157	14	infection	infection	NOUN
njb-39	157	15	that	that	PRON
njb-39	157	16	consequently	consequently	ADV
njb-39	157	17	would	would	AUX
njb-39	157	18	give	give	VERB
njb-39	157	19	a	a	DET
njb-39	157	20	positive	positive	ADJ
njb-39	157	21	signal	signal	NOUN
njb-39	157	22	in	in	ADP
njb-39	157	23	real	real	ADJ
njb-39	157	24	-	-	PUNCT
njb-39	157	25	time	time	NOUN
njb-39	157	26	pcr	pcr	NOUN
njb-39	157	27	can	can	AUX
njb-39	157	28	not	not	PART
njb-39	157	29	be	be	AUX
njb-39	157	30	converted	convert	VERB
njb-39	157	31	to	to	ADP
njb-39	157	32	viral	viral	ADJ
njb-39	157	33	titer	titer	NOUN
njb-39	157	34	,	,	PUNCT
njb-39	157	35	as	as	SCONJ
njb-39	157	36	this	this	PRON
njb-39	157	37	would	would	AUX
njb-39	157	38	give	give	VERB
njb-39	157	39	a	a	DET
njb-39	157	40	false	false	ADJ
njb-39	157	41	impression	impression	NOUN
njb-39	157	42	that	that	SCONJ
njb-39	157	43	the	the	DET
njb-39	157	44	virus	virus	NOUN
njb-39	157	45	is	be	AUX
njb-39	157	46	able	able	ADJ
njb-39	157	47	to	to	PART
njb-39	157	48	cause	cause	VERB
njb-39	157	49	cytopathic	cytopathic	ADJ
njb-39	157	50	effect	effect	NOUN
njb-39	157	51	.	.	PUNCT
njb-39	158	1	our	our	PRON
njb-39	158	2	data	datum	NOUN
njb-39	158	3	also	also	ADV
njb-39	158	4	demonstrated	demonstrate	VERB
njb-39	158	5	that	that	SCONJ
njb-39	158	6	using	use	VERB
njb-39	158	7	real	real	ADJ
njb-39	158	8	-	-	PUNCT
njb-39	158	9	time	time	NOUN
njb-39	158	10	pcr	pcr	PROPN
njb-39	158	11	technology	technology	NOUN
njb-39	158	12	is	be	AUX
njb-39	158	13	a	a	DET
njb-39	158	14	reliable	reliable	ADJ
njb-39	158	15	,	,	PUNCT
njb-39	158	16	rapid	rapid	ADJ
njb-39	158	17	and	and	CCONJ
njb-39	158	18	robust	robust	ADJ
njb-39	158	19	method	method	NOUN
njb-39	158	20	that	that	PRON
njb-39	158	21	corresponds	correspond	VERB
njb-39	158	22	to	to	ADP
njb-39	158	23	standard	standard	ADJ
njb-39	158	24	quantification	quantification	NOUN
njb-39	158	25	methods	method	NOUN
njb-39	158	26	used	use	VERB
njb-39	158	27	to	to	PART
njb-39	158	28	measure	measure	VERB
njb-39	158	29	viral	viral	ADJ
njb-39	158	30	titers	titer	NOUN
njb-39	158	31	.	.	PUNCT
njb-39	159	1	thus	thus	ADV
njb-39	159	2	,	,	PUNCT
njb-39	159	3	highly	highly	ADV
njb-39	159	4	reproducible	reproducible	VERB
njb-39	159	5	over	over	ADP
njb-39	159	6	a	a	DET
njb-39	159	7	dynamic	dynamic	ADJ
njb-39	159	8	range	range	NOUN
njb-39	159	9	of	of	ADP
njb-39	159	10	viral	viral	ADJ
njb-39	159	11	concentrations	concentration	NOUN
njb-39	159	12	commonly	commonly	ADV
njb-39	159	13	used	use	VERB
njb-39	159	14	in	in	ADP
njb-39	159	15	research	research	NOUN
njb-39	159	16	laboratories	laboratory	NOUN
njb-39	159	17	and	and	CCONJ
njb-39	159	18	could	could	AUX
njb-39	159	19	therefore	therefore	ADV
njb-39	159	20	save	save	VERB
njb-39	159	21	time	time	NOUN
njb-39	159	22	in	in	ADP
njb-39	159	23	comparison	comparison	NOUN
njb-39	159	24	with	with	ADP
njb-39	159	25	the	the	DET
njb-39	159	26	traditional	traditional	ADJ
njb-39	159	27	cell	cell	NOUN
njb-39	159	28	culture	culture	NOUN
njb-39	159	29	-	-	PUNCT
njb-39	159	30	based	base	VERB
njb-39	159	31	viral	viral	ADJ
njb-39	159	32	titration	titration	NOUN
njb-39	159	33	methods	method	NOUN
njb-39	159	34	.	.	PUNCT
njb-39	160	1	the	the	DET
njb-39	160	2	reproducibility	reproducibility	NOUN
njb-39	160	3	of	of	ADP
njb-39	160	4	quantitative	quantitative	ADJ
njb-39	160	5	real	real	ADJ
njb-39	160	6	-	-	PUNCT
njb-39	160	7	time	time	NOUN
njb-39	160	8	pcr	pcr	NOUN
njb-39	160	9	was	be	AUX
njb-39	160	10	higher	high	ADJ
njb-39	160	11	than	than	ADP
njb-39	160	12	for	for	ADP
njb-39	160	13	tcid50	tcid50	NOUN
njb-39	160	14	,	,	PUNCT
njb-39	160	15	which	which	PRON
njb-39	160	16	could	could	AUX
njb-39	160	17	be	be	AUX
njb-39	160	18	partially	partially	ADV
njb-39	160	19	explained	explain	VERB
njb-39	160	20	by	by	ADP
njb-39	160	21	the	the	DET
njb-39	160	22	fact	fact	NOUN
njb-39	160	23	that	that	SCONJ
njb-39	160	24	real	real	ADJ
njb-39	160	25	-	-	PUNCT
njb-39	160	26	time	time	NOUN
njb-39	160	27	pcr	pcr	NOUN
njb-39	160	28	does	do	AUX
njb-39	160	29	not	not	PART
njb-39	160	30	require	require	VERB
njb-39	160	31	a	a	DET
njb-39	160	32	manual	manual	ADJ
njb-39	160	33	estimation	estimation	NOUN
njb-39	160	34	based	base	VERB
njb-39	160	35	on	on	ADP
njb-39	160	36	visual	visual	ADJ
njb-39	160	37	observations	observation	NOUN
njb-39	160	38	.	.	PUNCT
njb-39	161	1	conclusion	conclusion	NOUN
njb-39	161	2	the	the	DET
njb-39	161	3	present	present	ADJ
njb-39	161	4	study	study	NOUN
njb-39	161	5	could	could	AUX
njb-39	161	6	successfully	successfully	ADV
njb-39	161	7	lyophilize	lyophilize	VERB
njb-39	161	8	adenovirus	adenovirus	NOUN
njb-39	161	9	and	and	CCONJ
njb-39	161	10	retain	retain	VERB
njb-39	161	11	its	its	PRON
njb-39	161	12	infectivity	infectivity	NOUN
njb-39	161	13	over	over	ADP
njb-39	161	14	a	a	DET
njb-39	161	15	period	period	NOUN
njb-39	161	16	of	of	ADP
njb-39	161	17	6	6	NUM
njb-39	161	18	months	month	NOUN
njb-39	161	19	when	when	SCONJ
njb-39	161	20	stored	store	VERB
njb-39	161	21	at	at	ADP
njb-39	161	22	rt	rt	PROPN
njb-39	161	23	and	and	CCONJ
njb-39	161	24	4	4	NUM
njb-39	161	25	°	°	NOUN
njb-39	161	26	c	c	NOUN
njb-39	161	27	.	.	PUNCT
njb-39	162	1	no	no	DET
njb-39	162	2	significant	significant	ADJ
njb-39	162	3	difference	difference	NOUN
njb-39	162	4	in	in	ADP
njb-39	162	5	the	the	DET
njb-39	162	6	infectivity	infectivity	NOUN
njb-39	162	7	or	or	CCONJ
njb-39	162	8	tcid50	tcid50	NOUN
njb-39	162	9	titer	titer	NOUN
njb-39	162	10	was	be	AUX
njb-39	162	11	observed	observe	VERB
njb-39	162	12	in	in	ADP
njb-39	162	13	the	the	DET
njb-39	162	14	lyophilized	lyophilize	VERB
njb-39	162	15	virus	virus	NOUN
njb-39	162	16	as	as	SCONJ
njb-39	162	17	compared	compare	VERB
njb-39	162	18	to	to	ADP
njb-39	162	19	the	the	DET
njb-39	162	20	stock	stock	NOUN
njb-39	162	21	virus	virus	NOUN
njb-39	162	22	.	.	PUNCT
njb-39	163	1	however	however	ADV
njb-39	163	2	,	,	PUNCT
njb-39	163	3	the	the	DET
njb-39	163	4	viral	viral	ADJ
njb-39	163	5	load	load	NOUN
njb-39	163	6	was	be	AUX
njb-39	163	7	observed	observe	VERB
njb-39	163	8	to	to	PART
njb-39	163	9	increase	increase	VERB
njb-39	163	10	with	with	ADP
njb-39	163	11	lyophilization	lyophilization	NOUN
njb-39	163	12	of	of	ADP
njb-39	163	13	the	the	DET
njb-39	163	14	virus	virus	NOUN
njb-39	163	15	over	over	ADP
njb-39	163	16	6	6	NUM
njb-39	163	17	months	month	NOUN
njb-39	163	18	when	when	SCONJ
njb-39	163	19	stored	store	VERB
njb-39	163	20	at	at	ADP
njb-39	163	21	4	4	NUM
njb-39	163	22	°	°	NOUN
njb-39	163	23	c	c	NOUN
njb-39	163	24	which	which	PRON
njb-39	163	25	possibly	possibly	ADV
njb-39	163	26	is	be	AUX
njb-39	163	27	due	due	ADJ
njb-39	163	28	to	to	ADP
njb-39	163	29	the	the	DET
njb-39	163	30	concentration	concentration	NOUN
njb-39	163	31	of	of	ADP
njb-39	163	32	the	the	DET
njb-39	163	33	virus	virus	NOUN
njb-39	163	34	on	on	ADP
njb-39	163	35	freeze	freeze	NOUN
njb-39	163	36	-	-	PUNCT
njb-39	163	37	drying	drying	NOUN
njb-39	163	38	.	.	PUNCT
njb-39	164	1	competing	compete	VERB
njb-39	164	2	interest	interest	NOUN
njb-39	164	3	the	the	DET
njb-39	164	4	authors	author	NOUN
njb-39	164	5	declare	declare	VERB
njb-39	164	6	no	no	DET
njb-39	164	7	competing	compete	VERB
njb-39	164	8	interest	interest	NOUN
njb-39	164	9	.	.	PUNCT
njb-39	165	1	authors	author	NOUN
njb-39	165	2	contribution	contribution	VERB
njb-39	165	3	bimlesh	bimlesh	PROPN
njb-39	165	4	kumar	kumar	PROPN
njb-39	165	5	jha	jha	PROPN
njb-39	165	6	designed	design	VERB
njb-39	165	7	the	the	DET
njb-39	165	8	experiment	experiment	NOUN
njb-39	165	9	.	.	PUNCT
njb-39	166	1	bimlesh	bimlesh	PROPN
njb-39	166	2	kumar	kumar	PROPN
njb-39	166	3	jha	jha	PROPN
njb-39	166	4	,	,	PUNCT
njb-39	166	5	birendra	birendra	PROPN
njb-39	166	6	prasad	prasad	PROPN
njb-39	166	7	gupta	gupta	NOUN
njb-39	166	8	,	,	PUNCT
njb-39	166	9	prashanna	prashanna	PROPN
njb-39	166	10	maharjan	maharjan	PROPN
njb-39	166	11	,	,	PUNCT
njb-39	166	12	somila	somila	PROPN
njb-39	166	13	kakshapati	kakshapati	PROPN
njb-39	166	14	and	and	CCONJ
njb-39	166	15	nabin	nabin	PROPN
njb-39	166	16	narayan	narayan	PROPN
njb-39	166	17	munankarmi	munankarmi	PROPN
njb-39	166	18	performed	perform	VERB
njb-39	166	19	the	the	DET
njb-39	166	20	cloning	cloning	NOUN
njb-39	166	21	and	and	CCONJ
njb-39	166	22	transfection	transfection	NOUN
njb-39	166	23	experiment	experiment	NOUN
njb-39	166	24	.	.	PUNCT
njb-39	167	1	bimlesh	bimlesh	PROPN
njb-39	167	2	kumar	kumar	PROPN
njb-39	167	3	jha	jha	PROPN
njb-39	167	4	,	,	PUNCT
njb-39	167	5	prashanna	prashanna	PROPN
njb-39	167	6	maharjan	maharjan	PROPN
njb-39	167	7	,	,	PUNCT
njb-39	167	8	somila	somila	PROPN
njb-39	167	9	kakshapati	kakshapati	PROPN
njb-39	167	10	,	,	PUNCT
njb-39	167	11	nabin	nabin	PROPN
njb-39	167	12	narayan	narayan	PROPN
njb-39	167	13	munankarmi	munankarmi	PROPN
njb-39	167	14	and	and	CCONJ
njb-39	167	15	birendra	birendra	PROPN
njb-39	167	16	prasad	prasad	PROPN
njb-39	167	17	gupta	gupta	NOUN
njb-39	167	18	performed	perform	VERB
njb-39	167	19	the	the	DET
njb-39	167	20	virus	virus	NOUN
njb-39	167	21	titration	titration	NOUN
njb-39	167	22	experiment	experiment	NOUN
njb-39	167	23	and	and	CCONJ
njb-39	167	24	data	datum	NOUN
njb-39	167	25	analysis	analysis	NOUN
njb-39	167	26	.	.	PUNCT
njb-39	168	1	bimlesh	bimlesh	PROPN
njb-39	168	2	kumar	kumar	PROPN
njb-39	168	3	jha	jha	PROPN
njb-39	168	4	,	,	PUNCT
njb-39	168	5	prashanna	prashanna	PROPN
njb-39	168	6	maharjan	maharjan	PROPN
njb-39	168	7	,	,	PUNCT
njb-39	168	8	somila	somila	PROPN
njb-39	168	9	kakshapati	kakshapati	PROPN
njb-39	168	10	nabin	nabin	PROPN
njb-39	168	11	narayan	narayan	PROPN
njb-39	168	12	munankarmi	munankarmi	PROPN
njb-39	168	13	and	and	CCONJ
njb-39	168	14	birendra	birendra	PROPN
njb-39	168	15	prasad	prasad	PROPN
njb-39	168	16	gupta	gupta	NOUN
njb-39	168	17	wrote	write	VERB
njb-39	168	18	the	the	DET
njb-39	168	19	manuscript	manuscript	NOUN
njb-39	168	20	.	.	PUNCT
njb-39	169	1	acknowledgement	acknowledgement	VERB
njb-39	169	2	we	we	PRON
njb-39	169	3	would	would	AUX
njb-39	169	4	like	like	VERB
njb-39	169	5	to	to	PART
njb-39	169	6	thank	thank	VERB
njb-39	169	7	national	national	PROPN
njb-39	169	8	institute	institute	PROPN
njb-39	169	9	of	of	ADP
njb-39	169	10	virology	virology	PROPN
njb-39	169	11	(	(	PUNCT
njb-39	169	12	niv	niv	PROPN
njb-39	169	13	)	)	PUNCT
njb-39	169	14	pune	pune	NOUN
njb-39	169	15	for	for	ADP
njb-39	169	16	providing	provide	VERB
njb-39	169	17	us	we	PRON
njb-39	169	18	viral	viral	ADJ
njb-39	169	19	stock	stock	NOUN
njb-39	169	20	.	.	PUNCT
njb-39	170	1	reference	reference	NOUN
njb-39	170	2	1	1	NUM
njb-39	170	3	.	.	PUNCT
njb-39	170	4	walls	wall	VERB
njb-39	170	5	t	t	PROPN
njb-39	170	6	,	,	PUNCT
njb-39	170	7	shankar	shankar	PROPN
njb-39	170	8	ag	ag	PROPN
njb-39	170	9	,	,	PUNCT
njb-39	170	10	shingadia	shingadia	PROPN
njb-39	170	11	d	d	PROPN
njb-39	170	12	:	:	PUNCT
njb-39	170	13	adenovirus	adenovirus	NOUN
njb-39	170	14	:	:	PUNCT
njb-39	170	15	an	an	DET
njb-39	170	16	increasingly	increasingly	ADV
njb-39	170	17	important	important	ADJ
njb-39	170	18	pathogen	pathogen	NOUN
njb-39	170	19	in	in	ADP
njb-39	170	20	paediatric	paediatric	ADJ
njb-39	170	21	bone	bone	NOUN
njb-39	170	22	marrow	marrow	NOUN
njb-39	170	23	transplant	transplant	NOUN
njb-39	170	24	patients	patient	NOUN
njb-39	170	25	.	.	PUNCT
njb-39	171	1	lancet	lancet	PROPN
njb-39	171	2	infect	infect	PROPN
njb-39	171	3	dis	dis	PROPN
njb-39	171	4	2003	2003	NUM
njb-39	171	5	,	,	PUNCT
njb-39	171	6	3(2):79	3(2):79	NUM
njb-39	171	7	-	-	SYM
njb-39	171	8	86	86	NUM
njb-39	171	9	.	.	NUM
njb-39	172	1	2	2	NUM
njb-39	172	2	.	.	PROPN
njb-39	172	3	croyle	croyle	PROPN
njb-39	172	4	ma	ma	PROPN
njb-39	172	5	,	,	PUNCT
njb-39	172	6	cheng	cheng	PROPN
njb-39	172	7	x	x	PROPN
njb-39	172	8	,	,	PUNCT
njb-39	172	9	wilson	wilson	PROPN
njb-39	172	10	jm	jm	PROPN
njb-39	172	11	:	:	PUNCT
njb-39	172	12	development	development	NOUN
njb-39	172	13	of	of	ADP
njb-39	172	14	formulations	formulation	NOUN
njb-39	172	15	that	that	PRON
njb-39	172	16	enhance	enhance	VERB
njb-39	172	17	physical	physical	ADJ
njb-39	172	18	stability	stability	NOUN
njb-39	172	19	of	of	ADP
njb-39	172	20	viral	viral	ADJ
njb-39	172	21	vectors	vector	NOUN
njb-39	172	22	for	for	ADP
njb-39	172	23	gene	gene	NOUN
njb-39	172	24	therapy	therapy	NOUN
njb-39	172	25	.	.	PUNCT
njb-39	173	1	gene	gene	NOUN
njb-39	173	2	ther	ther	NOUN
njb-39	173	3	2001	2001	NUM
njb-39	173	4	,	,	PUNCT
njb-39	173	5	8(17):12811290	8(17):12811290	NUM
njb-39	173	6	.	.	PUNCT
njb-39	174	1	3	3	X
njb-39	174	2	.	.	X
njb-39	174	3	nail	nail	PROPN
njb-39	174	4	sl	sl	PROPN
njb-39	174	5	,	,	PUNCT
njb-39	174	6	jiang	jiang	PROPN
njb-39	174	7	s	s	PROPN
njb-39	174	8	,	,	PUNCT
njb-39	174	9	chongprasert	chongprasert	PROPN
njb-39	174	10	s	s	PROPN
njb-39	174	11	,	,	PUNCT
njb-39	174	12	knopp	knopp	PROPN
njb-39	174	13	sa	sa	PROPN
njb-39	174	14	:	:	PUNCT
njb-39	174	15	fundamentals	fundamental	NOUN
njb-39	174	16	of	of	ADP
njb-39	174	17	freeze	freeze	NOUN
njb-39	174	18	-	-	PUNCT
njb-39	174	19	drying	drying	NOUN
njb-39	174	20	.	.	PUNCT
njb-39	175	1	pharm	pharm	NOUN
njb-39	175	2	biotechnol	biotechnol	NOUN
njb-39	175	3	2002	2002	NUM
njb-39	175	4	,	,	PUNCT
njb-39	175	5	14:281	14:281	NUM
njb-39	175	6	-	-	SYM
njb-39	175	7	360	360	NUM
njb-39	175	8	.	.	NOUN
njb-39	175	9	4	4	NUM
njb-39	175	10	.	.	PROPN
njb-39	175	11	schersch	schersch	PROPN
njb-39	175	12	k	k	PROPN
njb-39	175	13	,	,	PUNCT
njb-39	175	14	betz	betz	PROPN
njb-39	175	15	o	o	PROPN
njb-39	175	16	,	,	PUNCT
njb-39	175	17	garidel	garidel	PROPN
njb-39	175	18	p	p	PROPN
njb-39	175	19	,	,	PUNCT
njb-39	175	20	muehlau	muehlau	PROPN
njb-39	175	21	s	s	PROPN
njb-39	175	22	,	,	PUNCT
njb-39	175	23	bassarab	bassarab	PROPN
njb-39	175	24	s	s	PROPN
njb-39	175	25	,	,	PUNCT
njb-39	175	26	winter	winter	NOUN
njb-39	175	27	g	g	NOUN
njb-39	175	28	:	:	PUNCT
njb-39	175	29	systematic	systematic	ADJ
njb-39	175	30	investigation	investigation	NOUN
njb-39	175	31	of	of	ADP
njb-39	175	32	the	the	DET
njb-39	175	33	effect	effect	NOUN
njb-39	175	34	of	of	ADP
njb-39	175	35	lyophilizate	lyophilizate	ADJ
njb-39	175	36	collapse	collapse	NOUN
njb-39	175	37	on	on	ADP
njb-39	175	38	pharmaceutically	pharmaceutically	ADV
njb-39	175	39	relevant	relevant	ADJ
njb-39	175	40	proteins	protein	NOUN
njb-39	175	41	i	i	PRON
njb-39	175	42	:	:	PUNCT
njb-39	175	43	stability	stability	NOUN
njb-39	175	44	after	after	ADP
njb-39	175	45	freeze	freeze	NOUN
njb-39	175	46	-	-	PUNCT
njb-39	175	47	drying	drying	NOUN
njb-39	175	48	.	.	PUNCT
njb-39	176	1	j	j	PROPN
njb-39	176	2	pharm	pharm	PROPN
njb-39	176	3	sci	sci	PROPN
njb-39	176	4	2010	2010	NUM
njb-39	176	5	,	,	PUNCT
njb-39	176	6	99(5):2256	99(5):2256	NUM
njb-39	176	7	-	-	SYM
njb-39	176	8	2278	2278	NUM
njb-39	176	9	.	.	PUNCT
njb-39	177	1	5	5	X
njb-39	177	2	.	.	X
njb-39	177	3	chen	chen	PROPN
njb-39	177	4	s	s	PROPN
njb-39	177	5	,	,	PUNCT
njb-39	177	6	guo	guo	PROPN
njb-39	177	7	d	d	PROPN
njb-39	177	8	,	,	PUNCT
njb-39	177	9	guo	guo	PROPN
njb-39	177	10	b	b	PROPN
njb-39	177	11	,	,	PUNCT
njb-39	177	12	liu	liu	PROPN
njb-39	177	13	j	j	PROPN
njb-39	177	14	,	,	PUNCT
njb-39	177	15	shen	shen	PROPN
njb-39	177	16	y	y	PROPN
njb-39	177	17	,	,	PUNCT
njb-39	177	18	xu	xu	PROPN
njb-39	177	19	x	x	PROPN
njb-39	177	20	,	,	PUNCT
njb-39	177	21	huang	huang	PROPN
njb-39	177	22	w	w	PROPN
njb-39	177	23	,	,	PUNCT
njb-39	177	24	guo	guo	PROPN
njb-39	177	25	s	s	PROPN
njb-39	177	26	:	:	PUNCT
njb-39	177	27	investigation	investigation	NOUN
njb-39	177	28	on	on	ADP
njb-39	177	29	formulation	formulation	NOUN
njb-39	177	30	and	and	CCONJ
njb-39	177	31	preparation	preparation	NOUN
njb-39	177	32	of	of	ADP
njb-39	177	33	adenovirus	adenovirus	NOUN
njb-39	177	34	encoding	encode	VERB
njb-39	177	35	human	human	PROPN
njb-39	177	36	endostatin	endostatin	PROPN
njb-39	177	37	lyophilized	lyophilize	VERB
njb-39	177	38	powders	powder	NOUN
njb-39	177	39	.	.	PUNCT
njb-39	178	1	int	int	PROPN
njb-39	178	2	j	j	PROPN
njb-39	178	3	pharm	pharm	PROPN
njb-39	178	4	2012	2012	NUM
njb-39	178	5	,	,	PUNCT
njb-39	178	6	427(2):145	427(2):145	NUM
njb-39	178	7	-	-	SYM
njb-39	178	8	152	152	NUM
njb-39	178	9	.	.	NUM
njb-39	178	10	6	6	NUM
njb-39	178	11	.	.	PUNCT
njb-39	178	12	mcclure	mcclure	PROPN
njb-39	178	13	c	c	PROPN
njb-39	178	14	,	,	PUNCT
njb-39	178	15	cole	cole	PROPN
njb-39	178	16	kl	kl	PROPN
njb-39	178	17	,	,	PUNCT
njb-39	178	18	wulff	wulff	PROPN
njb-39	178	19	p	p	PROPN
njb-39	178	20	,	,	PUNCT
njb-39	178	21	klugmann	klugmann	PROPN
njb-39	178	22	m	m	PROPN
njb-39	178	23	,	,	PUNCT
njb-39	178	24	murray	murray	PROPN
njb-39	178	25	aj	aj	PROPN
njb-39	178	26	:	:	PUNCT
njb-39	178	27	production	production	NOUN
njb-39	178	28	and	and	CCONJ
njb-39	178	29	titering	titere	VERB
njb-39	178	30	of	of	ADP
njb-39	178	31	recombinant	recombinant	ADJ
njb-39	178	32	adenoassociated	adenoassociated	ADJ
njb-39	178	33	viral	viral	ADJ
njb-39	178	34	vectors	vector	NOUN
njb-39	178	35	.	.	PUNCT
njb-39	179	1	j	j	PROPN
njb-39	179	2	vis	vis	X
njb-39	179	3	exp	exp	PROPN
njb-39	179	4	2011(57):e3348	2011(57):e3348	PROPN
njb-39	179	5	.	.	PROPN
njb-39	179	6	7	7	NUM
njb-39	179	7	.	.	PUNCT
njb-39	179	8	xiao	xiao	PROPN
njb-39	179	9	x	x	PROPN
njb-39	179	10	,	,	PUNCT
njb-39	179	11	li	li	PROPN
njb-39	179	12	j	j	PROPN
njb-39	179	13	,	,	PUNCT
njb-39	179	14	samulski	samulski	PROPN
njb-39	179	15	rj	rj	PROPN
njb-39	179	16	:	:	PUNCT
njb-39	179	17	production	production	NOUN
njb-39	179	18	of	of	ADP
njb-39	179	19	high	high	ADJ
njb-39	179	20	-	-	PUNCT
njb-39	179	21	titer	titer	NOUN
njb-39	179	22	recombinant	recombinant	ADJ
njb-39	179	23	adeno	adeno	NOUN
njb-39	179	24	-	-	PUNCT
njb-39	179	25	associated	associate	VERB
njb-39	179	26	virus	virus	NOUN
njb-39	179	27	vectors	vector	NOUN
njb-39	179	28	in	in	ADP
njb-39	179	29	the	the	DET
njb-39	179	30	absence	absence	NOUN
njb-39	179	31	of	of	ADP
njb-39	179	32	helper	helper	NOUN
njb-39	179	33	adenovirus	adenovirus	NOUN
njb-39	179	34	.	.	PUNCT
njb-39	180	1	j	j	PROPN
njb-39	180	2	virol	virol	PROPN
njb-39	180	3	1998	1998	NUM
njb-39	180	4	,	,	PUNCT
njb-39	180	5	72(3):22242232	72(3):22242232	PROPN
njb-39	180	6	.	.	PROPN
njb-39	180	7	8	8	NUM
njb-39	180	8	.	.	PUNCT
njb-39	181	1	aurnhammer	aurnhammer	PROPN
njb-39	181	2	c	c	PROPN
njb-39	181	3	,	,	PUNCT
njb-39	181	4	haase	haase	PROPN
njb-39	181	5	m	m	PROPN
njb-39	181	6	,	,	PUNCT
njb-39	181	7	muether	muether	PROPN
njb-39	181	8	n	n	PROPN
njb-39	181	9	,	,	PUNCT
njb-39	181	10	hausl	hausl	PROPN
njb-39	181	11	m	m	PROPN
njb-39	181	12	,	,	PUNCT
njb-39	181	13	rauschhuber	rauschhuber	PROPN
njb-39	181	14	c	c	PROPN
njb-39	181	15	,	,	PUNCT
njb-39	181	16	huber	huber	PROPN
njb-39	181	17	i	i	PROPN
njb-39	181	18	,	,	PUNCT
njb-39	181	19	nitschko	nitschko	PROPN
njb-39	181	20	h	h	PROPN
njb-39	181	21	,	,	PUNCT
njb-39	181	22	busch	busch	PROPN
njb-39	181	23	u	u	PROPN
njb-39	181	24	,	,	PUNCT
njb-39	181	25	sing	sing	VERB
njb-39	181	26	a	a	DET
njb-39	181	27	,	,	PUNCT
njb-39	181	28	ehrhardt	ehrhardt	NOUN
njb-39	181	29	a	a	DET
njb-39	181	30	et	et	NOUN
njb-39	181	31	al	al	NOUN
njb-39	181	32	:	:	PUNCT
njb-39	181	33	universal	universal	ADJ
njb-39	181	34	real	real	ADJ
njb-39	181	35	-	-	PUNCT
njb-39	181	36	time	time	NOUN
njb-39	181	37	pcr	pcr	NOUN
njb-39	181	38	for	for	ADP
njb-39	181	39	the	the	DET
njb-39	181	40	detection	detection	NOUN
njb-39	181	41	and	and	CCONJ
njb-39	181	42	quantification	quantification	NOUN
njb-39	181	43	of	of	ADP
njb-39	181	44	adeno	adeno	NOUN
njb-39	181	45	-	-	PUNCT
njb-39	181	46	associated	associate	VERB
njb-39	181	47	nepal	nepal	ADJ
njb-39	181	48	journal	journal	NOUN
njb-39	181	49	of	of	ADP
njb-39	181	50	biotechnology	biotechnology	NOUN
njb-39	181	51	.	.	PUNCT
njb-39	182	1	d	d	X
njb-39	182	2	e	e	PROPN
njb-39	182	3	c	c	PROPN
njb-39	182	4	.	.	PUNCT
njb-39	183	1	2	2	NUM
njb-39	183	2	0	0	NUM
njb-39	183	3	1	1	NUM
njb-39	183	4	5	5	NUM
njb-39	183	5	vol	vol	NOUN
njb-39	183	6	.	.	PUNCT
njb-39	184	1	3	3	NUM
njb-39	184	2	,	,	PUNCT
njb-39	184	3	no	no	NOUN
njb-39	184	4	.	.	NOUN
njb-39	184	5	1:15	1:15	NUM
njb-39	184	6	-	-	SYM
njb-39	184	7	21	21	NUM
njb-39	184	8	jha	jha	NOUN
njb-39	184	9	et	et	PROPN
njb-39	184	10	al	al	PROPN
njb-39	184	11	.	.	PUNCT
njb-39	185	1	©	©	PROPN
njb-39	185	2	njb	njb	PROPN
njb-39	185	3	,	,	PUNCT
njb-39	185	4	biotechnology	biotechnology	NOUN
njb-39	185	5	society	society	NOUN
njb-39	185	6	of	of	ADP
njb-39	185	7	nepal	nepal	ADJ
njb-39	185	8	21	21	NUM
njb-39	185	9	nepjol.info/index.php/njb	nepjol.info/index.php/njb	PROPN
njb-39	185	10	virus	virus	NOUN
njb-39	185	11	serotype	serotype	NOUN
njb-39	185	12	2	2	NUM
njb-39	185	13	-	-	PUNCT
njb-39	185	14	derived	derive	VERB
njb-39	185	15	inverted	inverted	ADJ
njb-39	185	16	terminal	terminal	ADJ
njb-39	185	17	repeat	repeat	NOUN
njb-39	185	18	sequences	sequence	NOUN
njb-39	185	19	.	.	PUNCT
njb-39	186	1	hum	hum	ADJ
njb-39	186	2	gene	gene	NOUN
njb-39	186	3	ther	ther	NOUN
njb-39	186	4	methods	method	NOUN
njb-39	186	5	2012	2012	NUM
njb-39	186	6	,	,	PUNCT
njb-39	186	7	23(1):18	23(1):18	NUM
njb-39	186	8	-	-	SYM
njb-39	186	9	28	28	NUM
njb-39	186	10	.	.	NOUN
njb-39	186	11	9	9	NUM
njb-39	186	12	.	.	PUNCT
njb-39	187	1	grigorov	grigorov	PROPN
njb-39	187	2	b	b	PROPN
njb-39	187	3	,	,	PUNCT
njb-39	187	4	rabilloud	rabilloud	PROPN
njb-39	187	5	j	j	PROPN
njb-39	187	6	,	,	PUNCT
njb-39	187	7	lawrence	lawrence	PROPN
njb-39	187	8	p	p	PROPN
njb-39	187	9	,	,	PUNCT
njb-39	187	10	gerlier	gerli	ADJ
njb-39	187	11	d	d	NOUN
njb-39	187	12	:	:	PUNCT
njb-39	187	13	rapid	rapid	ADJ
njb-39	187	14	titration	titration	NOUN
njb-39	187	15	of	of	ADP
njb-39	187	16	measles	measle	NOUN
njb-39	187	17	and	and	CCONJ
njb-39	187	18	other	other	ADJ
njb-39	187	19	viruses	virus	NOUN
njb-39	187	20	:	:	PUNCT
njb-39	187	21	optimization	optimization	NOUN
njb-39	187	22	with	with	ADP
njb-39	187	23	determination	determination	NOUN
njb-39	187	24	of	of	ADP
njb-39	187	25	replication	replication	NOUN
njb-39	187	26	cycle	cycle	NOUN
njb-39	187	27	length	length	NOUN
njb-39	187	28	.	.	PUNCT
njb-39	188	1	plos	plos	PROPN
njb-39	188	2	one	one	NUM
njb-39	188	3	2011	2011	NUM
njb-39	188	4	,	,	PUNCT
njb-39	188	5	6(9):e24135	6(9):e24135	NUM
njb-39	188	6	.	.	PROPN
njb-39	188	7	10	10	NUM
njb-39	188	8	.	.	PUNCT
njb-39	189	1	biacchesi	biacchesi	PROPN
njb-39	189	2	s	s	PROPN
njb-39	189	3	,	,	PUNCT
njb-39	189	4	skiadopoulos	skiadopoulos	PROPN
njb-39	189	5	mh	mh	PROPN
njb-39	189	6	,	,	PUNCT
njb-39	189	7	yang	yang	PROPN
njb-39	189	8	l	l	PROPN
njb-39	189	9	,	,	PUNCT
njb-39	189	10	murphy	murphy	PROPN
njb-39	189	11	br	br	PROPN
njb-39	189	12	,	,	PUNCT
njb-39	189	13	collins	collins	PROPN
njb-39	189	14	pl	pl	PROPN
njb-39	189	15	,	,	PUNCT
njb-39	189	16	buchholz	buchholz	PROPN
njb-39	189	17	uj	uj	PROPN
njb-39	189	18	:	:	PUNCT
njb-39	189	19	rapid	rapid	ADJ
njb-39	189	20	human	human	ADJ
njb-39	189	21	metapneumovirus	metapneumovirus	NOUN
njb-39	189	22	microneutralization	microneutralization	NOUN
njb-39	189	23	assay	assay	NOUN
njb-39	189	24	based	base	VERB
njb-39	189	25	on	on	ADP
njb-39	189	26	green	green	ADJ
njb-39	189	27	fluorescent	fluorescent	NOUN
njb-39	189	28	protein	protein	NOUN
njb-39	189	29	expression	expression	NOUN
njb-39	189	30	.	.	PUNCT
njb-39	190	1	j	j	PROPN
njb-39	190	2	virol	virol	PROPN
njb-39	190	3	methods	methods	PROPN
njb-39	190	4	2005	2005	NUM
njb-39	190	5	,	,	PUNCT
njb-39	190	6	128(1	128(1	NUM
njb-39	190	7	-	-	PUNCT
njb-39	190	8	2):192	2):192	NUM
njb-39	190	9	-	-	PUNCT
njb-39	190	10	197	197	NUM
njb-39	190	11	.	.	PUNCT