id sid tid token lemma pos njb-47 1 1 microsoft microsoft PROPN njb-47 1 2 word word PROPN njb-47 1 3 paper paper NOUN njb-47 1 4 3 3 NUM njb-47 1 5 two two NUM njb-47 1 6 colum colum NOUN njb-47 1 7 final.docx final.docx ADP njb-47 1 8 nepal nepal ADJ njb-47 1 9     SPACE njb-47 1 10 journal journal NOUN njb-47 1 11     SPACE njb-47 1 12 of of ADP njb-47 1 13     SPACE njb-47 1 14 biotechnology biotechnology NOUN njb-47 1 15 . . PUNCT njb-47 1 16         SPACE njb-47 2 1 jan jan PROPN njb-47 2 2 . . PROPN njb-47 2 3     SPACE njb-47 2 4 2012 2012 NUM njb-47 2 5 , , PUNCT njb-47 2 6     SPACE njb-47 2 7 vol vol NOUN njb-47 2 8 . . PUNCT njb-47 2 9     SPACE njb-47 2 10 2 2 NUM njb-47 2 11 , , PUNCT njb-47 2 12     SPACE njb-47 2 13 no no INTJ njb-47 2 14 . . PUNCT njb-47 2 15     SPACE njb-47 3 1 1 1 NUM njb-47 3 2 : : PUNCT njb-47 3 3     SPACE njb-47 3 4 26 26 NUM njb-47 3 5     SPACE njb-47 3 6 – – PUNCT njb-47 3 7     SPACE njb-47 3 8 36 36 NUM njb-47 3 9                                                                                                                                                                                                                                             SPACE njb-47 3 10 biotechnology biotechnology NOUN njb-47 3 11     SPACE njb-47 3 12 society society NOUN njb-47 3 13     SPACE njb-47 3 14 of of ADP njb-47 3 15     SPACE njb-47 3 16 nepal nepal NOUN njb-47 3 17     SPACE njb-47 3 18 ( ( PUNCT njb-47 3 19 bsn bsn PROPN njb-47 3 20 ) ) PUNCT njb-47 3 21 , , PUNCT njb-47 3 22     SPACE njb-47 3 23 all all DET njb-47 3 24     SPACE njb-47 3 25 rights right NOUN njb-47 3 26     SPACE njb-47 3 27 reserved reserve VERB njb-47 3 28                 SPACE njb-47 3 29 26 26 NUM njb-47 3 30         SPACE njb-47 3 31 original original ADJ njb-47 3 32     SPACE njb-47 3 33 research research NOUN njb-47 3 34     SPACE njb-47 3 35 article article NOUN njb-47 3 36     SPACE njb-47 3 37 lovastatin lovastatin VERB njb-47 3 38     SPACE njb-47 3 39 production production NOUN njb-47 3 40     SPACE njb-47 3 41 by by ADP njb-47 3 42     SPACE njb-47 3 43 aspergillus aspergillus NOUN njb-47 3 44     SPACE njb-47 3 45 fischeri fischeri PROPN njb-47 3 46     SPACE njb-47 3 47 under under ADP njb-47 3 48     SPACE njb-47 3 49 solid solid ADJ njb-47 3 50     SPACE njb-47 3 51 state state NOUN njb-47 3 52     SPACE njb-47 3 53 fermentation fermentation NOUN njb-47 3 54     SPACE njb-47 3 55 from from ADP njb-47 3 56     SPACE njb-47 3 57 coconut coconut NOUN njb-47 3 58     SPACE njb-47 3 59 oil oil NOUN njb-47 3 60     SPACE njb-47 3 61 cake cake NOUN njb-47 3 62     SPACE njb-47 3 63 parvatham parvatham PROPN njb-47 3 64 madhu madhu PROPN njb-47 3 65 latha1 latha1 PROPN njb-47 3 66 * * PROPN njb-47 3 67 , , PUNCT njb-47 3 68 , , PUNCT njb-47 3 69 pallem pallem PROPN njb-47 3 70 chanakya2 chanakya2 PROPN njb-47 3 71 , , PUNCT njb-47 3 72 manipati manipati PROPN njb-47 3 73 srikanth3 srikanth3 PROPN njb-47 3 74 1 1 NUM njb-47 3 75 , , PUNCT njb-47 3 76 * * PROPN njb-47 3 77 nri nri PROPN njb-47 3 78 college college PROPN njb-47 3 79 of of ADP njb-47 3 80 pharmacy pharmacy NOUN njb-47 3 81 , , PUNCT njb-47 3 82 pothavarappadu pothavarappadu ADJ njb-47 3 83 , , PUNCT njb-47 3 84 agiripalli agiripalli PROPN njb-47 3 85 mandal mandal PROPN njb-47 3 86 , , PUNCT njb-47 3 87 vijayawada vijayawada PROPN njb-47 3 88 rural rural ADJ njb-47 3 89 , , PUNCT njb-47 3 90 andhra andhra PROPN njb-47 3 91 pradesh pradesh NOUN njb-47 3 92 . . PUNCT njb-47 4 1 2centre 2centre NUM njb-47 4 2 for for ADP njb-47 4 3 biotechnology biotechnology NOUN njb-47 4 4 , , PUNCT njb-47 4 5 department department NOUN njb-47 4 6 of of ADP njb-47 4 7 chemical chemical PROPN njb-47 4 8 engineering engineering NOUN njb-47 4 9 , , PUNCT njb-47 4 10 au au ADP njb-47 4 11 college college NOUN njb-47 4 12 of of ADP njb-47 4 13 engineering engineering NOUN njb-47 4 14 ( ( PUNCT njb-47 4 15 a a PRON njb-47 4 16 ) ) PUNCT njb-47 4 17 , , PUNCT njb-47 4 18 andhra andhra PROPN njb-47 4 19 university university PROPN njb-47 4 20 , , PUNCT njb-47 4 21 visakhapatnam visakhapatnam PROPN njb-47 4 22 , , PUNCT njb-47 4 23 andhra andhra PROPN njb-47 4 24 pradesh pradesh NOUN njb-47 4 25 . . PUNCT njb-47 5 1 3nri 3nri NUM njb-47 5 2 academy academy NOUN njb-47 5 3 of of ADP njb-47 5 4 medical medical PROPN njb-47 5 5 sciences sciences PROPN njb-47 5 6 , , PUNCT njb-47 5 7 china china PROPN njb-47 5 8 kakani kakani PROPN njb-47 5 9 , , PUNCT njb-47 5 10 mangalagiri mangalagiri PROPN njb-47 5 11 mandal mandal ADJ njb-47 5 12 , , PUNCT njb-47 5 13 guntur guntur PROPN njb-47 5 14 , , PUNCT njb-47 5 15 andhra andhra PROPN njb-47 5 16 pradesh pradesh NOUN njb-47 5 17 * * PUNCT njb-47 5 18 corresponding correspond VERB njb-47 5 19 author author NOUN njb-47 5 20 : : PUNCT njb-47 5 21 srimani.nri@gmail.com srimani.nri@gmail.com X njb-47 5 22 , , PUNCT njb-47 5 23 chanakya.pallem@gmail.com chanakya.pallem@gmail.com X njb-47 5 24 abstract abstract ADJ njb-47 5 25 : : PUNCT njb-47 5 26 the the DET njb-47 5 27 main main ADJ njb-47 5 28 aim aim NOUN njb-47 5 29 of of ADP njb-47 5 30 the the DET njb-47 5 31 present present ADJ njb-47 5 32 investigation investigation NOUN njb-47 5 33 was be AUX njb-47 5 34 to to PART njb-47 5 35 optimize optimize VERB njb-47 5 36 the the DET njb-47 5 37 fermentation fermentation NOUN njb-47 5 38 parameters parameter NOUN njb-47 5 39 that that PRON njb-47 5 40 enhance enhance VERB njb-47 5 41 the the DET njb-47 5 42 maximum maximum ADJ njb-47 5 43 production production NOUN njb-47 5 44 of of ADP njb-47 5 45 lovastatin lovastatin PROPN njb-47 5 46 by by ADP njb-47 5 47 aspergillus aspergillus PROPN njb-47 5 48 fischeri fischeri PROPN njb-47 5 49 using use VERB njb-47 5 50 coconut coconut NOUN njb-47 5 51 oil oil NOUN njb-47 5 52 cake cake NOUN njb-47 5 53 as as ADP njb-47 5 54 the the DET njb-47 5 55 solid solid ADJ njb-47 5 56 substrate substrate NOUN njb-47 5 57 under under ADP njb-47 5 58 solid solid ADJ njb-47 5 59 state state NOUN njb-47 5 60 fermentation fermentation NOUN njb-47 5 61 . . PUNCT njb-47 6 1 the the DET njb-47 6 2 maximum maximum ADJ njb-47 6 3 yield yield NOUN njb-47 6 4 of of ADP njb-47 6 5 lovastatin lovastatin PROPN njb-47 6 6 ( ( PUNCT njb-47 6 7 14.77 14.77 NUM njb-47 6 8 mg mg PROPN njb-47 6 9 / / SYM njb-47 6 10 g g NOUN njb-47 6 11 dry dry ADJ njb-47 6 12 substrate substrate NOUN njb-47 6 13 ) ) PUNCT njb-47 6 14 using use VERB njb-47 6 15 coconut coconut NOUN njb-47 6 16 oil oil NOUN njb-47 6 17 cake cake NOUN njb-47 6 18 as as SCONJ njb-47 6 19 the the DET njb-47 6 20 substrate substrate NOUN njb-47 6 21 was be AUX njb-47 6 22 achieved achieve VERB njb-47 6 23 with with ADP njb-47 6 24 the the DET njb-47 6 25 following following ADJ njb-47 6 26 optimized optimize VERB njb-47 6 27 process process NOUN njb-47 6 28 parameters parameter NOUN njb-47 6 29 : : PUNCT njb-47 6 30 fermentation fermentation NOUN njb-47 6 31 time time NOUN njb-47 6 32 ( ( PUNCT njb-47 6 33 7 7 NUM njb-47 6 34 days day NOUN njb-47 6 35 ) ) PUNCT njb-47 6 36 , , PUNCT njb-47 6 37 initial initial ADJ njb-47 6 38 moisture moisture NOUN njb-47 6 39 content content NOUN njb-47 6 40 ( ( PUNCT njb-47 6 41 60 60 NUM njb-47 6 42 % % NOUN njb-47 6 43 v v NOUN njb-47 6 44 / / SYM njb-47 6 45 w w NOUN njb-47 6 46 ) ) PUNCT njb-47 6 47 , , PUNCT njb-47 6 48 inoculum inoculum PROPN njb-47 6 49 volume volume NOUN njb-47 6 50 ( ( PUNCT njb-47 6 51 2ml 2ml NOUN njb-47 6 52 of of ADP njb-47 6 53 five five NUM njb-47 6 54 day day NOUN njb-47 6 55 old old ADJ njb-47 6 56 culture culture NOUN njb-47 6 57 ) ) PUNCT njb-47 6 58 , , PUNCT njb-47 6 59 initial initial ADJ njb-47 6 60 ph ph NOUN njb-47 6 61 ( ( PUNCT njb-47 6 62 5.0 5.0 NUM njb-47 6 63 ) ) PUNCT njb-47 6 64 , , PUNCT njb-47 6 65 incubation incubation NOUN njb-47 6 66 temperature temperature NOUN njb-47 6 67 ( ( PUNCT njb-47 6 68 30ºc 30ºc NOUN njb-47 6 69 ) ) PUNCT njb-47 6 70 , , PUNCT njb-47 6 71 lactose lactose NOUN njb-47 6 72 ( ( PUNCT njb-47 6 73 1 1 NUM njb-47 6 74 % % NOUN njb-47 6 75 w w NOUN njb-47 6 76 / / SYM njb-47 6 77 v v NOUN njb-47 6 78 ) ) PUNCT njb-47 6 79 and and CCONJ njb-47 6 80 malt malt NOUN njb-47 6 81 extract extract NOUN njb-47 6 82 ( ( PUNCT njb-47 6 83 1 1 NUM njb-47 6 84 % % NOUN njb-47 6 85 w w NOUN njb-47 6 86 / / SYM njb-47 6 87 v v NOUN njb-47 6 88 ) ) PUNCT njb-47 6 89 . . PUNCT njb-47 7 1 keywords keyword NOUN njb-47 7 2 : : PUNCT njb-47 7 3 lovastatin lovastatin PROPN njb-47 7 4 , , PUNCT njb-47 7 5 aspergillus aspergillus PROPN njb-47 7 6 fischeri fischeri PROPN njb-47 7 7 , , PUNCT njb-47 7 8 coconut coconut NOUN njb-47 7 9 oil oil NOUN njb-47 7 10 cake cake NOUN njb-47 7 11 , , PUNCT njb-47 7 12 fermentation fermentation NOUN njb-47 7 13 parameters parameter NOUN njb-47 7 14 , , PUNCT njb-47 7 15 optimization optimization NOUN njb-47 7 16 introduction introduction NOUN njb-47 7 17 : : PUNCT njb-47 7 18 lovastatin lovastatin PROPN njb-47 7 19 also also ADV njb-47 7 20 called call VERB njb-47 7 21 as as ADP njb-47 7 22 mevinolin mevinolin ADJ njb-47 7 23 , , PUNCT njb-47 7 24 monacolin monacolin PROPN njb-47 7 25 k k PROPN njb-47 7 26 and and CCONJ njb-47 7 27 mevacor mevacor PROPN njb-47 7 28 , , PUNCT njb-47 7 29 a a DET njb-47 7 30 kind kind NOUN njb-47 7 31 of of ADP njb-47 7 32 fungal fungal ADJ njb-47 7 33 metabolite metabolite PROPN njb-47 7 34 , , PUNCT njb-47 7 35 serves serve VERB njb-47 7 36 as as ADP njb-47 7 37 one one NUM njb-47 7 38 of of ADP njb-47 7 39 the the DET njb-47 7 40 competitive competitive ADJ njb-47 7 41 inhibitors inhibitor NOUN njb-47 7 42 of of ADP njb-47 7 43 enzyme enzyme NOUN njb-47 7 44 hydroxymethylglutaryl hydroxymethylglutaryl NOUN njb-47 7 45 coenzyme coenzyme PROPN njb-47 7 46 a a DET njb-47 7 47 reductase reductase NOUN njb-47 7 48 ( ( PUNCT njb-47 7 49 hmg hmg NOUN njb-47 7 50 - - PUNCT njb-47 7 51 coa coa PROPN njb-47 7 52 ) ) PUNCT njb-47 7 53 , , PUNCT njb-47 7 54 which which PRON njb-47 7 55 catalyzes catalyze VERB njb-47 7 56 the the DET njb-47 7 57 rate rate NOUN njb-47 7 58 - - PUNCT njb-47 7 59 limiting limit VERB njb-47 7 60 step step NOUN njb-47 7 61 in in ADP njb-47 7 62 cholesterol cholesterol NOUN njb-47 7 63 biosynthesis biosynthesis NOUN njb-47 7 64 , , PUNCT njb-47 7 65 resulting result VERB njb-47 7 66 in in ADP njb-47 7 67 lowered lower VERB njb-47 7 68 blood blood NOUN njb-47 7 69 cholesterol cholesterol NOUN njb-47 7 70 [ [ PUNCT njb-47 7 71 1,2 1,2 NUM njb-47 7 72 ] ] PUNCT njb-47 7 73 . . PUNCT njb-47 8 1 in in ADP njb-47 8 2 addition addition NOUN njb-47 8 3 , , PUNCT njb-47 8 4 lovastatin lovastatin PROPN njb-47 8 5 has have AUX njb-47 8 6 been be AUX njb-47 8 7 used use VERB njb-47 8 8 in in ADP njb-47 8 9 the the DET njb-47 8 10 biomedical biomedical ADJ njb-47 8 11 applications application NOUN njb-47 8 12 such such ADJ njb-47 8 13 as as ADP njb-47 8 14 , , PUNCT njb-47 8 15 in in ADP njb-47 8 16 treating treat VERB njb-47 8 17 coronary coronary ADJ njb-47 8 18 heart heart NOUN njb-47 8 19 diseases disease NOUN njb-47 8 20 , , PUNCT njb-47 8 21 renal renal ADJ njb-47 8 22 diseases disease NOUN njb-47 8 23 , , PUNCT njb-47 8 24 alzheimer alzheimer NOUN njb-47 8 25 ’s ’s PART njb-47 8 26 disease disease NOUN njb-47 8 27 , , PUNCT njb-47 8 28 bone bone NOUN njb-47 8 29 fractions fraction NOUN njb-47 8 30 [ [ X njb-47 8 31 3 3 NUM njb-47 8 32 ] ] X njb-47 8 33 . . PUNCT njb-47 9 1 moreover moreover ADV njb-47 9 2 , , PUNCT njb-47 9 3 it it PRON njb-47 9 4 has have AUX njb-47 9 5 been be AUX njb-47 9 6 indicated indicate VERB njb-47 9 7 as as ADP njb-47 9 8 a a DET njb-47 9 9 potential potential ADJ njb-47 9 10 therapeutic therapeutic ADJ njb-47 9 11 agent agent NOUN njb-47 9 12 for for ADP njb-47 9 13 the the DET njb-47 9 14 treatment treatment NOUN njb-47 9 15 of of ADP njb-47 9 16 various various ADJ njb-47 9 17 types type NOUN njb-47 9 18 of of ADP njb-47 9 19 tumors tumor NOUN njb-47 9 20 because because SCONJ njb-47 9 21 of of ADP njb-47 9 22 its its PRON njb-47 9 23 capability capability NOUN njb-47 9 24 to to PART njb-47 9 25 suppress suppress VERB njb-47 9 26 tumor tumor NOUN njb-47 9 27 growth growth NOUN njb-47 9 28 in in ADP njb-47 9 29 vivo vivo NOUN njb-47 9 30 through through ADP njb-47 9 31 inhibition inhibition NOUN njb-47 9 32 of of ADP njb-47 9 33 the the DET njb-47 9 34 synthesis synthesis NOUN njb-47 9 35 of of ADP njb-47 9 36 nonsterol nonsterol PROPN njb-47 9 37 isoprenoid isoprenoid PROPN njb-47 9 38 compounds compound NOUN njb-47 10 1 [ [ X njb-47 10 2 4 4 NUM njb-47 10 3 , , PUNCT njb-47 10 4 5 5 NUM njb-47 10 5 ] ] PUNCT njb-47 10 6 . . PUNCT njb-47 11 1 commercial commercial ADJ njb-47 11 2 production production NOUN njb-47 11 3 of of ADP njb-47 11 4 lovastatin lovastatin PROPN njb-47 11 5 has have AUX njb-47 11 6 been be AUX njb-47 11 7 carried carry VERB njb-47 11 8 out out ADP njb-47 11 9 by by ADP njb-47 11 10 submerged submerged ADJ njb-47 11 11 fermentation fermentation NOUN njb-47 11 12 using use VERB njb-47 11 13 different different ADJ njb-47 11 14 microorganisms microorganism NOUN njb-47 11 15 [ [ PUNCT njb-47 11 16 6 6 NUM njb-47 11 17 , , PUNCT njb-47 11 18 7 7 NUM njb-47 11 19 ] ] PUNCT njb-47 11 20 . . PUNCT njb-47 12 1 in in ADP njb-47 12 2 recent recent ADJ njb-47 12 3 nepal nepal ADJ njb-47 12 4     SPACE njb-47 12 5 journal journal NOUN njb-47 12 6     SPACE njb-47 12 7 of of ADP njb-47 12 8     SPACE njb-47 12 9 biotechnology biotechnology NOUN njb-47 12 10 . . PUNCT njb-47 12 11         SPACE njb-47 13 1 jan jan PROPN njb-47 13 2 . . PROPN njb-47 13 3     SPACE njb-47 13 4 2012 2012 NUM njb-47 13 5 , , PUNCT njb-47 13 6     SPACE njb-47 13 7 vol vol NOUN njb-47 13 8 . . PUNCT njb-47 13 9     SPACE njb-47 13 10 2 2 NUM njb-47 13 11 , , PUNCT njb-47 13 12     SPACE njb-47 13 13 no no INTJ njb-47 13 14 . . PUNCT njb-47 13 15     SPACE njb-47 14 1 1 1 NUM njb-47 14 2 : : PUNCT njb-47 14 3     SPACE njb-47 14 4 26 26 NUM njb-47 14 5     SPACE njb-47 14 6 – – PUNCT njb-47 14 7     SPACE njb-47 14 8 36 36 NUM njb-47 14 9                                                                                                                                                                                                                                             SPACE njb-47 14 10 biotechnology biotechnology NOUN njb-47 14 11     SPACE njb-47 14 12 society society NOUN njb-47 14 13     SPACE njb-47 14 14 of of ADP njb-47 14 15     SPACE njb-47 14 16 nepal nepal NOUN njb-47 14 17     SPACE njb-47 14 18 ( ( PUNCT njb-47 14 19 bsn bsn PROPN njb-47 14 20 ) ) PUNCT njb-47 14 21 , , PUNCT njb-47 14 22     SPACE njb-47 14 23 all all DET njb-47 14 24     SPACE njb-47 14 25 rights right NOUN njb-47 14 26     SPACE njb-47 14 27 reserved reserve VERB njb-47 14 28                 SPACE njb-47 14 29 27 27 NUM njb-47 14 30     SPACE njb-47 14 31 years year NOUN njb-47 14 32 , , PUNCT njb-47 14 33 researchers researcher NOUN njb-47 14 34 have have AUX njb-47 14 35 shown show VERB njb-47 14 36 an an DET njb-47 14 37 increasing increase VERB njb-47 14 38 interest interest NOUN njb-47 14 39 in in ADP njb-47 14 40 solid solid ADJ njb-47 14 41 state state NOUN njb-47 14 42 fermentation fermentation NOUN njb-47 14 43 ( ( PUNCT njb-47 14 44 ssf ssf NOUN njb-47 14 45 ) ) PUNCT njb-47 14 46 as as SCONJ njb-47 14 47 it it PRON njb-47 14 48 is be AUX njb-47 14 49 a a DET njb-47 14 50 potential potential ADJ njb-47 14 51 alternative alternative NOUN njb-47 14 52 of of ADP njb-47 14 53 submerged submerged ADJ njb-47 14 54 fermentation fermentation NOUN njb-47 14 55 because because SCONJ njb-47 14 56 economical economical ADJ njb-47 14 57 and and CCONJ njb-47 14 58 cheap cheap ADJ njb-47 14 59 substrates substrate NOUN njb-47 14 60 can can AUX njb-47 14 61 be be AUX njb-47 14 62 utilized utilize VERB njb-47 14 63 in in ADP njb-47 14 64 ssf ssf NOUN njb-47 14 65 for for ADP njb-47 14 66 production production NOUN njb-47 14 67 of of ADP njb-47 14 68 value value NOUN njb-47 14 69 - - PUNCT njb-47 14 70 added add VERB njb-47 14 71 products product NOUN njb-47 14 72 , , PUNCT njb-47 14 73 and and CCONJ njb-47 14 74 also also ADV njb-47 14 75 requires require VERB njb-47 14 76 less less ADJ njb-47 14 77 energy energy NOUN njb-47 14 78 , , PUNCT njb-47 14 79 and and CCONJ njb-47 14 80 low low ADJ njb-47 14 81 capital capital NOUN njb-47 14 82 costs cost NOUN njb-47 14 83 . . PUNCT njb-47 15 1 now now ADV njb-47 15 2 days day NOUN njb-47 15 3 , , PUNCT njb-47 15 4 ssf ssf PROPN njb-47 15 5 has have AUX njb-47 15 6 been be AUX njb-47 15 7 exploited exploit VERB njb-47 15 8 for for ADP njb-47 15 9 the the DET njb-47 15 10 production production NOUN njb-47 15 11 of of ADP njb-47 15 12 highly highly ADV njb-47 15 13 potent potent ADJ njb-47 15 14 drugs drug NOUN njb-47 15 15 on on ADP njb-47 15 16 large large ADJ njb-47 15 17 scale scale NOUN njb-47 15 18 . . PUNCT njb-47 16 1 but but CCONJ njb-47 16 2 still still ADV njb-47 16 3 , , PUNCT njb-47 16 4 few few ADJ njb-47 16 5 reports report NOUN njb-47 16 6 are be AUX njb-47 16 7 available available ADJ njb-47 16 8 on on ADP njb-47 16 9 the the DET njb-47 16 10 microbial microbial ADJ njb-47 16 11 production production NOUN njb-47 16 12 of of ADP njb-47 16 13 lovastatin lovastatin PROPN njb-47 16 14 under under ADP njb-47 16 15 ssf ssf NOUN njb-47 17 1 [ [ PUNCT njb-47 17 2 8 8 NUM njb-47 17 3 - - SYM njb-47 17 4 10 10 NUM njb-47 17 5 ] ] PUNCT njb-47 17 6 . . PUNCT njb-47 18 1 in in ADP njb-47 18 2 the the DET njb-47 18 3 present present ADJ njb-47 18 4 study study NOUN njb-47 18 5 , , PUNCT njb-47 18 6 we we PRON njb-47 18 7 evaluated evaluate VERB njb-47 18 8 the the DET njb-47 18 9 feasibility feasibility NOUN njb-47 18 10 of of ADP njb-47 18 11 ssf ssf PROPN njb-47 18 12 process process NOUN njb-47 18 13 for for ADP njb-47 18 14 lovastatin lovastatin ADJ njb-47 18 15 production production NOUN njb-47 18 16 by by ADP njb-47 18 17 aspergillus aspergillus PROPN njb-47 18 18 fischeri fischeri PROPN njb-47 18 19 and and CCONJ njb-47 18 20 optimized optimize VERB njb-47 18 21 different different ADJ njb-47 18 22 process process NOUN njb-47 18 23 parameters parameter NOUN njb-47 18 24 that that PRON njb-47 18 25 maximize maximize VERB njb-47 18 26 the the DET njb-47 18 27 lovastatin lovastatin ADJ njb-47 18 28 yield yield NOUN njb-47 18 29 . . PUNCT njb-47 19 1 materials material NOUN njb-47 19 2 and and CCONJ njb-47 19 3 methods method NOUN njb-47 19 4 microorganism microorganism NOUN njb-47 19 5 and and CCONJ njb-47 19 6 inoculum inoculum PROPN njb-47 19 7 preparation preparation NOUN njb-47 19 8 : : PUNCT njb-47 19 9 the the DET njb-47 19 10 fungal fungal PROPN njb-47 19 11 strain strain PROPN njb-47 19 12 aspergillus aspergillus PROPN njb-47 19 13 fischeri fischeri PROPN njb-47 19 14 ncim ncim PROPN njb-47 19 15 509 509 NUM njb-47 19 16 used use VERB njb-47 19 17 in in ADP njb-47 19 18 this this DET njb-47 19 19 study study NOUN njb-47 19 20 was be AUX njb-47 19 21 procured procure VERB njb-47 19 22 from from ADP njb-47 19 23 the the DET njb-47 19 24 culture culture NOUN njb-47 19 25 collection collection NOUN njb-47 19 26 centre centre NOUN njb-47 19 27 of of ADP njb-47 19 28 national national ADJ njb-47 19 29 collection collection NOUN njb-47 19 30 of of ADP njb-47 19 31 industrial industrial ADJ njb-47 19 32 microorganisms microorganism NOUN njb-47 19 33 ( ( PUNCT njb-47 19 34 ncim ncim PROPN njb-47 19 35 ) ) PUNCT njb-47 19 36 , , PUNCT njb-47 19 37 ncl ncl PROPN njb-47 19 38 , , PUNCT njb-47 19 39 pune pune NOUN njb-47 19 40 . . PUNCT njb-47 20 1 the the DET njb-47 20 2 microbial microbial ADJ njb-47 20 3 strain strain NOUN njb-47 20 4 was be AUX njb-47 20 5 maintained maintain VERB njb-47 20 6 on on ADP njb-47 20 7 potato potato NOUN njb-47 20 8 dextrose dextrose NOUN njb-47 20 9 agar agar NOUN njb-47 20 10 ( ( PUNCT njb-47 20 11 pda pda PROPN njb-47 20 12 ) ) PUNCT njb-47 20 13 slants slant NOUN njb-47 20 14 containing contain VERB njb-47 20 15 ( ( PUNCT njb-47 20 16 g g NOUN njb-47 20 17 / / SYM njb-47 20 18 l l NOUN njb-47 20 19 ) ) PUNCT njb-47 20 20 dextrose dextrose NOUN njb-47 20 21 20.0 20.0 NUM njb-47 20 22 and and CCONJ njb-47 20 23 agar agar VERB njb-47 20 24 15.0 15.0 NUM njb-47 20 25 . . PUNCT njb-47 21 1 inoculated inoculated ADJ njb-47 21 2 slants slant NOUN njb-47 21 3 were be AUX njb-47 21 4 grown grow VERB njb-47 21 5 in in ADP njb-47 21 6 an an DET njb-47 21 7 incubator incubator NOUN njb-47 21 8 at at ADP njb-47 21 9 28 28 NUM njb-47 21 10 ° ° NOUN njb-47 21 11 c c NOUN njb-47 21 12 for for ADP njb-47 21 13 5 5 NUM njb-47 21 14 days day NOUN njb-47 21 15 . . PUNCT njb-47 22 1 after after ADP njb-47 22 2 that that PRON njb-47 22 3 , , PUNCT njb-47 22 4 the the DET njb-47 22 5 slants slant NOUN njb-47 22 6 were be AUX njb-47 22 7 preserved preserve VERB njb-47 22 8 at at ADP njb-47 22 9 4 4 NUM njb-47 22 10 ° ° NOUN njb-47 22 11 c c NOUN njb-47 22 12 and and CCONJ njb-47 22 13 sub sub ADJ njb-47 22 14 - - ADJ njb-47 22 15 cultured cultured ADJ njb-47 22 16 once once ADV njb-47 22 17 every every DET njb-47 22 18 four four NUM njb-47 22 19 weeks week NOUN njb-47 22 20 ( ( PUNCT njb-47 22 21 monthly monthly ADJ njb-47 22 22 ) ) PUNCT njb-47 22 23 . . PUNCT njb-47 23 1 inoculum inoculum PROPN njb-47 23 2 preparation preparation PROPN njb-47 23 3 : : PUNCT njb-47 23 4 inoculum inoculum PROPN njb-47 23 5 was be AUX njb-47 23 6 prepared prepare VERB njb-47 23 7 by by ADP njb-47 23 8 adding add VERB njb-47 23 9 10 10 NUM njb-47 23 10 ml ml NOUN njb-47 23 11 of of ADP njb-47 23 12 sterilized sterilize VERB njb-47 23 13 0.1 0.1 NUM njb-47 23 14 % % NOUN njb-47 23 15 tween tween ADP njb-47 23 16 80 80 NUM njb-47 23 17 solution solution NOUN njb-47 23 18 to to ADP njb-47 23 19 a a DET njb-47 23 20 wellsporulated wellsporulated ADJ njb-47 23 21 a. a. NOUN njb-47 23 22 fischeri fischeri PROPN njb-47 23 23 slant slant NOUN njb-47 23 24 . . PUNCT njb-47 24 1 the the DET njb-47 24 2 spore spore ADJ njb-47 24 3 surface surface NOUN njb-47 24 4 was be AUX njb-47 24 5 scrapped scrap VERB njb-47 24 6 with with ADP njb-47 24 7 an an DET njb-47 24 8 inoculating inoculate VERB njb-47 24 9 loop loop NOUN njb-47 24 10 to to PART njb-47 24 11 suspend suspend VERB njb-47 24 12 the the DET njb-47 24 13 spores spore NOUN njb-47 24 14 in in ADP njb-47 24 15 the the DET njb-47 24 16 solution solution NOUN njb-47 24 17 and and CCONJ njb-47 24 18 the the DET njb-47 24 19 obtained obtain VERB njb-47 24 20 spore spore ADJ njb-47 24 21 suspension suspension NOUN njb-47 24 22 was be AUX njb-47 24 23 used use VERB njb-47 24 24 as as ADP njb-47 24 25 the the DET njb-47 24 26 inoculum inoculum NOUN njb-47 24 27 for for ADP njb-47 24 28 the the DET njb-47 24 29 fermentation fermentation NOUN njb-47 24 30 process process NOUN njb-47 24 31 . . PUNCT njb-47 25 1 solid solid ADJ njb-47 25 2 state state NOUN njb-47 25 3 fermentation fermentation NOUN njb-47 25 4 ( ( PUNCT njb-47 25 5 ssf ssf NOUN njb-47 25 6 ): ): PUNCT njb-47 25 7 coconut coconut NOUN njb-47 25 8 oil oil NOUN njb-47 25 9 cake cake NOUN njb-47 25 10 obtained obtain VERB njb-47 25 11 from from ADP njb-47 25 12 the the DET njb-47 25 13 local local ADJ njb-47 25 14 market market NOUN njb-47 25 15 of of ADP njb-47 25 16 vijayawada vijayawada PROPN njb-47 25 17 , , PUNCT njb-47 25 18 andhra andhra PROPN njb-47 25 19 pradesh pradesh PROPN njb-47 25 20 was be AUX njb-47 25 21 used use VERB njb-47 25 22 as as ADP njb-47 25 23 the the DET njb-47 25 24 substrate substrate NOUN njb-47 25 25 in in ADP njb-47 25 26 the the DET njb-47 25 27 present present ADJ njb-47 25 28 study study NOUN njb-47 25 29 . . PUNCT njb-47 26 1 five five NUM njb-47 26 2 grams gram NOUN njb-47 26 3 of of ADP njb-47 26 4 coconut coconut NOUN njb-47 26 5 oil oil NOUN njb-47 26 6 cake cake NOUN njb-47 26 7 was be AUX njb-47 26 8 dried dry VERB njb-47 26 9 and and CCONJ njb-47 26 10 grounded ground VERB njb-47 26 11 to to ADP njb-47 26 12 required require VERB njb-47 26 13 particle particle NOUN njb-47 26 14 size size NOUN njb-47 26 15 having have VERB njb-47 26 16 both both CCONJ njb-47 26 17 coarse coarse ADJ njb-47 26 18 and and CCONJ njb-47 26 19 fine fine ADJ njb-47 26 20 particles particle NOUN njb-47 26 21 in in ADP njb-47 26 22 1:1 1:1 NUM njb-47 26 23 ratio ratio NOUN njb-47 26 24 ( ( PUNCT njb-47 26 25 w w PROPN njb-47 26 26 / / SYM njb-47 26 27 w w PROPN njb-47 26 28 ) ) PUNCT njb-47 26 29 . . PUNCT njb-47 27 1 the the DET njb-47 27 2 substrate substrate NOUN njb-47 27 3 was be AUX njb-47 27 4 taken take VERB njb-47 27 5 in in ADP njb-47 27 6 a a DET njb-47 27 7 250ml 250ml NOUN njb-47 27 8 erlenmeyer erlenmeyer PROPN njb-47 27 9 flask flask PROPN njb-47 27 10 and and CCONJ njb-47 27 11 moistened moisten VERB njb-47 27 12 with with ADP njb-47 27 13 distilled distil VERB njb-47 27 14 water water NOUN njb-47 27 15 to to PART njb-47 27 16 maintain maintain VERB njb-47 27 17 the the DET njb-47 27 18 moisture moisture NOUN njb-47 27 19 content content NOUN njb-47 27 20 of of ADP njb-47 27 21 50 50 NUM njb-47 27 22 % % NOUN njb-47 27 23 ( ( PUNCT njb-47 27 24 v v NOUN njb-47 27 25 / / SYM njb-47 27 26 w w NOUN njb-47 27 27 ) ) PUNCT njb-47 27 28 and and CCONJ njb-47 27 29 autoclaved autoclave VERB njb-47 27 30 at at ADP njb-47 27 31 121ºc 121ºc PROPN njb-47 27 32 ( ( PUNCT njb-47 27 33 15 15 NUM njb-47 27 34 lb lb NOUN njb-47 27 35 ) ) PUNCT njb-47 27 36 for for ADP njb-47 27 37 1520 1520 NUM njb-47 27 38 min min NOUN njb-47 27 39 , , PUNCT njb-47 27 40 cooled cool VERB njb-47 27 41 to to ADP njb-47 27 42 room room NOUN njb-47 27 43 temperature temperature NOUN njb-47 27 44 and and CCONJ njb-47 27 45 inoculated inoculate VERB njb-47 27 46 with with ADP njb-47 27 47 2 2 NUM njb-47 27 48 ml ml NOUN njb-47 27 49 of of ADP njb-47 27 50 the the DET njb-47 27 51 5 5 NUM njb-47 27 52 day day NOUN njb-47 27 53 spore spore ADJ njb-47 27 54 suspension suspension NOUN njb-47 27 55 ( ( PUNCT njb-47 27 56 spore spore ADJ njb-47 27 57 concentration concentration NOUN njb-47 27 58 of of ADP njb-47 27 59 about about ADP njb-47 27 60 107 107 NUM njb-47 27 61 - - SYM njb-47 27 62 108 108 NUM njb-47 27 63 per per ADP njb-47 27 64 ml ml PROPN njb-47 27 65 ) ) PUNCT njb-47 27 66 of of ADP njb-47 27 67 a. a. PROPN njb-47 27 68 fischeri fischeri PROPN njb-47 27 69 under under ADP njb-47 27 70 aseptic aseptic ADJ njb-47 27 71 conditions condition NOUN njb-47 27 72 . . PUNCT njb-47 28 1 the the DET njb-47 28 2 contents content NOUN njb-47 28 3 of of ADP njb-47 28 4 the the DET njb-47 28 5 inoculated inoculated ADJ njb-47 28 6 flasks flask NOUN njb-47 28 7 were be AUX njb-47 28 8 mixed mix VERB njb-47 28 9 thoroughly thoroughly ADV njb-47 28 10 and and CCONJ njb-47 28 11 incubated incubate VERB njb-47 28 12 at at ADP njb-47 28 13 desired desire VERB njb-47 28 14 temperature temperature NOUN njb-47 28 15 in in ADP njb-47 28 16 an an DET njb-47 28 17 incubator incubator NOUN njb-47 28 18 for for ADP njb-47 28 19 desired desire VERB njb-47 28 20 length length NOUN njb-47 28 21 of of ADP njb-47 28 22 fermentation fermentation NOUN njb-47 28 23 time time NOUN njb-47 28 24 . . PUNCT njb-47 29 1 nepal nepal ADJ njb-47 29 2     SPACE njb-47 29 3 journal journal NOUN njb-47 29 4     SPACE njb-47 29 5 of of ADP njb-47 29 6     SPACE njb-47 29 7 biotechnology biotechnology NOUN njb-47 29 8 . . PUNCT njb-47 29 9         SPACE njb-47 30 1 jan jan PROPN njb-47 30 2 . . PROPN njb-47 30 3     SPACE njb-47 30 4 2012 2012 NUM njb-47 30 5 , , PUNCT njb-47 30 6     SPACE njb-47 30 7 vol vol NOUN njb-47 30 8 . . PUNCT njb-47 30 9     SPACE njb-47 30 10 2 2 NUM njb-47 30 11 , , PUNCT njb-47 30 12     SPACE njb-47 30 13 no no INTJ njb-47 30 14 . . PUNCT njb-47 30 15     SPACE njb-47 31 1 1 1 NUM njb-47 31 2 : : PUNCT njb-47 31 3     SPACE njb-47 31 4 26 26 NUM njb-47 31 5     SPACE njb-47 31 6 – – PUNCT njb-47 31 7     SPACE njb-47 31 8 36 36 NUM njb-47 31 9                                                                                                                                                                                                                                             SPACE njb-47 31 10 biotechnology biotechnology NOUN njb-47 31 11     SPACE njb-47 31 12 society society NOUN njb-47 31 13     SPACE njb-47 31 14 of of ADP njb-47 31 15     SPACE njb-47 31 16 nepal nepal NOUN njb-47 31 17     SPACE njb-47 31 18 ( ( PUNCT njb-47 31 19 bsn bsn PROPN njb-47 31 20 ) ) PUNCT njb-47 31 21 , , PUNCT njb-47 31 22     SPACE njb-47 31 23 all all DET njb-47 31 24     SPACE njb-47 31 25 rights right NOUN njb-47 31 26     SPACE njb-47 31 27 reserved reserve VERB njb-47 31 28                 SPACE njb-47 31 29 28 28 NUM njb-47 31 30     SPACE njb-47 31 31 optimization optimization NOUN njb-47 31 32 of of ADP njb-47 31 33 process process NOUN njb-47 31 34 parameters parameter NOUN njb-47 31 35 : : PUNCT njb-47 31 36 various various ADJ njb-47 31 37 process process NOUN njb-47 31 38 parameters parameter NOUN njb-47 31 39 that that PRON njb-47 31 40 influence influence VERB njb-47 31 41 the the DET njb-47 31 42 lovastatin lovastatin ADJ njb-47 31 43 production production NOUN njb-47 31 44 during during ADP njb-47 31 45 ssf ssf PROPN njb-47 31 46 were be AUX njb-47 31 47 optimized optimize VERB njb-47 31 48 over over ADP njb-47 31 49 a a DET njb-47 31 50 wide wide ADJ njb-47 31 51 range range NOUN njb-47 31 52 . . PUNCT njb-47 32 1 the the DET njb-47 32 2 strategy strategy NOUN njb-47 32 3 adopted adopt VERB njb-47 32 4 for for ADP njb-47 32 5 standardization standardization NOUN njb-47 32 6 of of ADP njb-47 32 7 fermentation fermentation NOUN njb-47 32 8 parameters parameter NOUN njb-47 32 9 was be AUX njb-47 32 10 to to PART njb-47 32 11 evaluate evaluate VERB njb-47 32 12 the the DET njb-47 32 13 effect effect NOUN njb-47 32 14 of of ADP njb-47 32 15 an an DET njb-47 32 16 individual individual ADJ njb-47 32 17 parameter parameter NOUN njb-47 32 18 and and CCONJ njb-47 32 19 incorporate incorporate VERB njb-47 32 20 it it PRON njb-47 32 21 at at ADP njb-47 32 22 the the DET njb-47 32 23 standard standard ADJ njb-47 32 24 level level NOUN njb-47 32 25 before before ADP njb-47 32 26 standardizing standardize VERB njb-47 32 27 the the DET njb-47 32 28 next next ADJ njb-47 32 29 parameter parameter NOUN njb-47 32 30 . . PUNCT njb-47 33 1 the the DET njb-47 33 2 process process NOUN njb-47 33 3 parameters parameter NOUN njb-47 33 4 optimized optimize VERB njb-47 33 5 in in ADP njb-47 33 6 the the DET njb-47 33 7 present present ADJ njb-47 33 8 study study NOUN njb-47 33 9 include include VERB njb-47 33 10 fermentation fermentation NOUN njb-47 33 11 time time NOUN njb-47 33 12 ( ( PUNCT njb-47 33 13 24 24 NUM njb-47 33 14 - - PUNCT njb-47 33 15 192h 192h NUM njb-47 33 16 ) ) PUNCT njb-47 33 17 , , PUNCT njb-47 33 18 initial initial ADJ njb-47 33 19 moisture moisture NOUN njb-47 33 20 content content NOUN njb-47 33 21 ( ( PUNCT njb-47 33 22 40 40 NUM njb-47 33 23 - - SYM njb-47 33 24 100 100 NUM njb-47 33 25 % % NOUN njb-47 33 26 v v NOUN njb-47 33 27 / / SYM njb-47 33 28 w w NOUN njb-47 33 29 ) ) PUNCT njb-47 33 30 , , PUNCT njb-47 33 31 inoculum inoculum PROPN njb-47 33 32 volume volume NOUN njb-47 33 33 ( ( PUNCT njb-47 33 34 0.5 0.5 NUM njb-47 33 35 - - PUNCT njb-47 33 36 4ml 4ml NOUN njb-47 33 37 ) ) PUNCT njb-47 33 38 , , PUNCT njb-47 33 39 initial initial ADJ njb-47 33 40 ph ph NOUN njb-47 33 41 ( ( PUNCT njb-47 33 42 4 4 NUM njb-47 33 43 - - SYM njb-47 33 44 10 10 NUM njb-47 33 45 ) ) PUNCT njb-47 33 46 , , PUNCT njb-47 33 47 incubation incubation NOUN njb-47 33 48 temperature temperature NOUN njb-47 33 49 ( ( PUNCT njb-47 33 50 26 26 NUM njb-47 33 51 - - SYM njb-47 33 52 40˚c 40˚c NUM njb-47 33 53 ) ) PUNCT njb-47 33 54 , , PUNCT njb-47 33 55 carbon carbon NOUN njb-47 33 56 sources source NOUN njb-47 33 57 ( ( PUNCT njb-47 33 58 1 1 NUM njb-47 33 59 % % NOUN njb-47 33 60 w w NOUN njb-47 33 61 / / SYM njb-47 33 62 v v NOUN njb-47 33 63 ) ) PUNCT njb-47 33 64 and and CCONJ njb-47 33 65 nitrogen nitrogen NOUN njb-47 33 66 sources source NOUN njb-47 33 67 ( ( PUNCT njb-47 33 68 1 1 NUM njb-47 33 69 % % NOUN njb-47 33 70 w w NOUN njb-47 33 71 / / SYM njb-47 33 72 v v NOUN njb-47 33 73 ) ) PUNCT njb-47 33 74 . . PUNCT njb-47 34 1 all all DET njb-47 34 2 the the DET njb-47 34 3 experiments experiment NOUN njb-47 34 4 were be AUX njb-47 34 5 done do VERB njb-47 34 6 in in ADP njb-47 34 7 triplicate triplicate NOUN njb-47 34 8 and and CCONJ njb-47 34 9 the the DET njb-47 34 10 mean mean ADJ njb-47 34 11 values value NOUN njb-47 34 12 of of ADP njb-47 34 13 the the DET njb-47 34 14 lovastatin lovastatin ADJ njb-47 34 15 yield yield NOUN njb-47 34 16 was be AUX njb-47 34 17 reported report VERB njb-47 34 18 . . PUNCT njb-47 35 1 lovastatin lovastatin ADJ njb-47 35 2 extraction extraction NOUN njb-47 35 3 : : PUNCT njb-47 35 4 after after ADP njb-47 35 5 the the DET njb-47 35 6 fermentation fermentation NOUN njb-47 35 7 time time NOUN njb-47 35 8 , , PUNCT njb-47 35 9 the the DET njb-47 35 10 fermented ferment VERB njb-47 35 11 flasks flask NOUN njb-47 35 12 were be AUX njb-47 35 13 dried dry VERB njb-47 35 14 at at ADP njb-47 35 15 60 60 NUM njb-47 35 16 ° ° NOUN njb-47 35 17 c c NOUN njb-47 35 18 for for ADP njb-47 35 19 2 2 NUM njb-47 35 20 hrs hrs NOUN njb-47 35 21 . . PUNCT njb-47 36 1 to to ADP njb-47 36 2 this this DET njb-47 36 3 20 20 NUM njb-47 36 4 ml ml NOUN njb-47 36 5 of of ADP njb-47 36 6 methanol methanol NOUN njb-47 36 7 was be AUX njb-47 36 8 added add VERB njb-47 36 9 to to ADP njb-47 36 10 the the DET njb-47 36 11 flasks flask NOUN njb-47 36 12 to to PART njb-47 36 13 extract extract VERB njb-47 36 14 lovastatin lovastatin NOUN njb-47 36 15 by by ADP njb-47 36 16 keeping keep VERB njb-47 36 17 them they PRON njb-47 36 18 in in ADP njb-47 36 19 an an DET njb-47 36 20 orbital orbital ADJ njb-47 36 21 shaker shaker NOUN njb-47 36 22 at at ADP njb-47 36 23 180 180 NUM njb-47 36 24 rpm rpm NOUN njb-47 36 25 for for ADP njb-47 36 26 2 2 NUM njb-47 36 27 h. h. NOUN njb-47 36 28 the the DET njb-47 36 29 residue residue NOUN njb-47 36 30 was be AUX njb-47 36 31 filtered filter VERB njb-47 36 32 with with ADP njb-47 36 33 filter filter NOUN njb-47 36 34 paper paper NOUN njb-47 36 35 and and CCONJ njb-47 36 36 then then ADV njb-47 36 37 centrifuged centrifuge VERB njb-47 36 38 at at ADP njb-47 36 39 1500 1500 NUM njb-47 36 40 rpm rpm NOUN njb-47 36 41 for for ADP njb-47 36 42 15 15 NUM njb-47 36 43 min min NOUN njb-47 36 44 . . PUNCT njb-47 37 1 the the DET njb-47 37 2 supernatant supernatant NOUN njb-47 37 3 obtained obtain VERB njb-47 37 4 was be AUX njb-47 37 5 collected collect VERB njb-47 37 6 and and CCONJ njb-47 37 7 analyzed analyze VERB njb-47 37 8 for for ADP njb-47 37 9 quantitative quantitative ADJ njb-47 37 10 determination determination NOUN njb-47 37 11 of of ADP njb-47 37 12 lovastatin lovastatin PROPN njb-47 37 13 [ [ X njb-47 37 14 10 10 NUM njb-47 37 15 ] ] PUNCT njb-47 37 16 . . PUNCT njb-47 38 1 quantitative quantitative ADJ njb-47 38 2 analysis analysis NOUN njb-47 38 3 of of ADP njb-47 38 4 lovastatin lovastatin NOUN njb-47 38 5 : : PUNCT njb-47 38 6 to to ADP njb-47 38 7 1 1 NUM njb-47 38 8 ml ml NOUN njb-47 38 9 of of ADP njb-47 38 10 the the DET njb-47 38 11 obtained obtain VERB njb-47 38 12 supernatant supernatant ADJ njb-47 38 13 , , PUNCT njb-47 38 14 1 1 NUM njb-47 38 15 ml ml NOUN njb-47 38 16 of of ADP njb-47 38 17 1 1 NUM njb-47 38 18 % % NOUN njb-47 38 19 trifloroacetic trifloroacetic ADJ njb-47 38 20 acid acid NOUN njb-47 38 21 was be AUX njb-47 38 22 added add VERB njb-47 38 23 and and CCONJ njb-47 38 24 incubated incubate VERB njb-47 38 25 for for ADP njb-47 38 26 10 10 NUM njb-47 38 27 min min NOUN njb-47 38 28 ( ( PUNCT njb-47 38 29 lactonization lactonization NOUN njb-47 38 30 of of ADP njb-47 38 31 hydroxyl hydroxyl PROPN njb-47 38 32 acid acid PROPN njb-47 38 33 form form NOUN njb-47 38 34 of of ADP njb-47 38 35 lovastatin).from lovastatin).from PROPN njb-47 38 36 the the DET njb-47 38 37 solution solution NOUN njb-47 38 38 , , PUNCT njb-47 38 39 0.5 0.5 NUM njb-47 38 40 ml ml NOUN njb-47 38 41 was be AUX njb-47 38 42 taken take VERB njb-47 38 43 and and CCONJ njb-47 38 44 diluted dilute VERB njb-47 38 45 to to ADP njb-47 38 46 10100 10100 NUM njb-47 38 47 ml ml NOUN njb-47 38 48 in in ADP njb-47 38 49 methanol methanol NOUN njb-47 38 50 and and CCONJ njb-47 38 51 its its PRON njb-47 38 52 absorbance absorbance NOUN njb-47 38 53 was be AUX njb-47 38 54 read read VERB njb-47 38 55 at at ADP njb-47 38 56 238 238 NUM njb-47 38 57 nm nm NOUN njb-47 38 58 by by ADP njb-47 38 59 using use VERB njb-47 38 60 uv uv NOUN njb-47 38 61 - - PUNCT njb-47 38 62 visible visible ADJ njb-47 38 63 spectrophotometer spectrophotometer NOUN njb-47 38 64 [ [ X njb-47 38 65 11 11 NUM njb-47 38 66 ] ] PUNCT njb-47 38 67 . . PUNCT njb-47 39 1 concentration concentration NOUN njb-47 39 2 of of ADP njb-47 39 3 lovastatin lovastatin PROPN njb-47 39 4 ( ( PUNCT njb-47 39 5 mg mg PROPN njb-47 39 6 / / SYM njb-47 39 7 g g NOUN njb-47 39 8 ) ) PUNCT njb-47 39 9 = = NOUN njb-47 39 10 concentration concentration NOUN njb-47 39 11 of of ADP njb-47 39 12 lovastatin lovastatin PROPN njb-47 39 13 ( ( PUNCT njb-47 39 14 mg mg PROPN njb-47 39 15 / / SYM njb-47 39 16 ml ml PROPN njb-47 39 17 ) ) PUNCT njb-47 39 18 x x X njb-47 40 1 dilution dilution NOUN njb-47 40 2 factor factor NOUN njb-47 40 3 ( ( PUNCT njb-47 40 4 df df NOUN njb-47 40 5 ) ) PUNCT njb-47 40 6 amount amount NOUN njb-47 40 7 of of ADP njb-47 40 8 substrate substrate NOUN njb-47 40 9 taken take VERB njb-47 40 10 ( ( PUNCT njb-47 40 11 g g X njb-47 40 12 ) ) PUNCT njb-47 40 13 results result NOUN njb-47 40 14 and and CCONJ njb-47 40 15 discussion discussion NOUN njb-47 40 16 selection selection NOUN njb-47 40 17 of of ADP njb-47 40 18 solid solid ADJ njb-47 40 19 substrates substrate NOUN njb-47 40 20 in in ADP njb-47 40 21 ssf ssf NOUN njb-47 40 22 for for ADP njb-47 40 23 lovastatin lovastatin ADJ njb-47 40 24 production production NOUN njb-47 40 25 : : PUNCT njb-47 40 26 solid solid ADJ njb-47 40 27 substrates substrate NOUN njb-47 40 28 employed employ VERB njb-47 40 29 in in ADP njb-47 40 30 ssf ssf NOUN njb-47 40 31 processes process NOUN njb-47 40 32 are be AUX njb-47 40 33 heterogeneous heterogeneous ADJ njb-47 40 34 natural natural ADJ njb-47 40 35 raw raw ADJ njb-47 40 36 - - PUNCT njb-47 40 37 materials material NOUN njb-47 40 38 which which PRON njb-47 40 39 are be AUX njb-47 40 40 generally generally ADV njb-47 40 41 insoluble insoluble ADJ njb-47 40 42 in in ADP njb-47 40 43 water water NOUN njb-47 40 44 and and CCONJ njb-47 40 45 play play VERB njb-47 40 46 a a DET njb-47 40 47 dual dual ADJ njb-47 40 48 role role NOUN njb-47 40 49 - - PUNCT njb-47 40 50 supply supply NOUN njb-47 40 51 of of ADP njb-47 40 52 nutrients nutrient NOUN njb-47 40 53 to to ADP njb-47 40 54 the the DET njb-47 40 55 microbial microbial ADJ njb-47 40 56 culture culture NOUN njb-47 40 57 growing grow VERB njb-47 40 58 and and CCONJ njb-47 40 59 anchorage anchorage VERB njb-47 40 60 for for ADP njb-47 40 61 the the DET njb-47 40 62 growing grow VERB njb-47 40 63 cells cell NOUN njb-47 40 64 . . PUNCT njb-47 41 1 in in ADP njb-47 41 2 the the DET njb-47 41 3 present present ADJ njb-47 41 4 study study NOUN njb-47 41 5 , , PUNCT njb-47 41 6 coconut coconut NOUN njb-47 41 7 oil oil NOUN njb-47 41 8 cake cake NOUN njb-47 41 9 was be AUX njb-47 41 10 used use VERB njb-47 41 11 as as ADP njb-47 41 12 the the DET njb-47 41 13 solid solid ADJ njb-47 41 14 substrate substrate NOUN njb-47 41 15 for for ADP njb-47 41 16 both both CCONJ njb-47 41 17 the the DET njb-47 41 18 fungal fungal ADJ njb-47 41 19 sporulation sporulation NOUN njb-47 41 20 and and CCONJ njb-47 41 21 lovastatin lovastatin ADJ njb-47 41 22 production production NOUN njb-47 41 23 by by ADP njb-47 41 24 aspergillus aspergillus PROPN njb-47 41 25 fischeri fischeri PROPN njb-47 41 26 . . PUNCT njb-47 42 1 maximum maximum PROPN njb-47 42 2 nepal nepal ADJ njb-47 42 3     SPACE njb-47 42 4 journal journal NOUN njb-47 42 5     SPACE njb-47 42 6 of of ADP njb-47 42 7     SPACE njb-47 42 8 biotechnology biotechnology NOUN njb-47 42 9 . . PUNCT njb-47 42 10         SPACE njb-47 43 1 jan jan PROPN njb-47 43 2 . . PROPN njb-47 43 3     SPACE njb-47 43 4 2012 2012 NUM njb-47 43 5 , , PUNCT njb-47 43 6     SPACE njb-47 43 7 vol vol NOUN njb-47 43 8 . . PUNCT njb-47 43 9     SPACE njb-47 43 10 2 2 NUM njb-47 43 11 , , PUNCT njb-47 43 12     SPACE njb-47 43 13 no no INTJ njb-47 43 14 . . PUNCT njb-47 43 15     SPACE njb-47 44 1 1 1 NUM njb-47 44 2 : : PUNCT njb-47 44 3     SPACE njb-47 44 4 26 26 NUM njb-47 44 5     SPACE njb-47 44 6 – – PUNCT njb-47 44 7     SPACE njb-47 44 8 36 36 NUM njb-47 44 9                                                                                                                                                                                                                                             SPACE njb-47 44 10 biotechnology biotechnology NOUN njb-47 44 11     SPACE njb-47 44 12 society society NOUN njb-47 44 13     SPACE njb-47 44 14 of of ADP njb-47 44 15     SPACE njb-47 44 16 nepal nepal NOUN njb-47 44 17     SPACE njb-47 44 18 ( ( PUNCT njb-47 44 19 bsn bsn PROPN njb-47 44 20 ) ) PUNCT njb-47 44 21 , , PUNCT njb-47 44 22     SPACE njb-47 44 23 all all DET njb-47 44 24     SPACE njb-47 44 25 rights right NOUN njb-47 44 26     SPACE njb-47 44 27 reserved reserve VERB njb-47 44 28                 SPACE njb-47 44 29 29 29 NUM njb-47 44 30     SPACE njb-47 44 31 lovastatin lovastatin ADJ njb-47 44 32 yield yield NOUN njb-47 44 33 of of ADP njb-47 44 34 6.81 6.81 NUM njb-47 44 35 mg mg PROPN njb-47 44 36 / / SYM njb-47 44 37 g g X njb-47 44 38 dry dry ADJ njb-47 44 39 substrate substrate NOUN njb-47 44 40 was be AUX njb-47 44 41 obtained obtain VERB njb-47 44 42 . . PUNCT njb-47 45 1 effect effect NOUN njb-47 45 2 of of ADP njb-47 45 3 fermentation fermentation NOUN njb-47 45 4 time time NOUN njb-47 45 5 : : PUNCT njb-47 45 6 the the DET njb-47 45 7 effect effect NOUN njb-47 45 8 of of ADP njb-47 45 9 fermentation fermentation NOUN njb-47 45 10 time time NOUN njb-47 45 11 on on ADP njb-47 45 12 lovastatin lovastatin ADJ njb-47 45 13 production production NOUN njb-47 45 14 was be AUX njb-47 45 15 studied study VERB njb-47 45 16 by by ADP njb-47 45 17 incubating incubate VERB njb-47 45 18 the the DET njb-47 45 19 fermentation fermentation NOUN njb-47 45 20 flasks flask NOUN njb-47 45 21 from from ADP njb-47 45 22 24 24 NUM njb-47 45 23 - - SYM njb-47 45 24 192 192 NUM njb-47 45 25 h. h. NOUN njb-47 45 26 the the DET njb-47 45 27 maximum maximum ADJ njb-47 45 28 lovastatin lovastatin ADJ njb-47 45 29 yield yield NOUN njb-47 45 30 of of ADP njb-47 45 31 7.43 7.43 NUM njb-47 45 32 mg mg PROPN njb-47 45 33 / / SYM njb-47 45 34 g g NOUN njb-47 45 35 of of ADP njb-47 45 36 dry dry ADJ njb-47 45 37 substrate substrate NOUN njb-47 45 38 was be AUX njb-47 45 39 achieved achieve VERB njb-47 45 40 at at ADP njb-47 45 41 168 168 NUM njb-47 45 42 h h NOUN njb-47 45 43 of of ADP njb-47 45 44 fermentation fermentation NOUN njb-47 45 45 time time NOUN njb-47 45 46 ( ( PUNCT njb-47 45 47 fig.1 fig.1 NOUN njb-47 45 48 ) ) PUNCT njb-47 45 49 . . PUNCT njb-47 46 1 the the DET njb-47 46 2 lovastatin lovastatin ADJ njb-47 46 3 yield yield NOUN njb-47 46 4 increased increase VERB njb-47 46 5 from from ADP njb-47 46 6 24 24 NUM njb-47 46 7 - - SYM njb-47 46 8 168 168 NUM njb-47 46 9 h h NOUN njb-47 46 10 which which PRON njb-47 46 11 explained explain VERB njb-47 46 12 that that SCONJ njb-47 46 13 lovastatin lovastatin PROPN njb-47 46 14 is be AUX njb-47 46 15 a a DET njb-47 46 16 kind kind NOUN njb-47 46 17 of of ADP njb-47 46 18 secondary secondary ADJ njb-47 46 19 metabolite metabolite NOUN njb-47 46 20 and and CCONJ njb-47 46 21 its its PRON njb-47 46 22 accumulation accumulation NOUN njb-47 46 23 in in ADP njb-47 46 24 mycelia mycelia NOUN njb-47 46 25 seems seem VERB njb-47 46 26 growth growth NOUN njb-47 46 27 related relate VERB njb-47 46 28 and and CCONJ njb-47 46 29 slightly slightly ADV njb-47 46 30 decreased decrease VERB njb-47 46 31 from from ADP njb-47 46 32 168 168 NUM njb-47 46 33 - - PUNCT njb-47 46 34 192h 192h NUM njb-47 46 35 . . PUNCT njb-47 47 1 the the DET njb-47 47 2 lovastatin lovastatin PROPN njb-47 47 3 yield yield NOUN njb-47 47 4 decreased decrease VERB njb-47 47 5 after after ADP njb-47 47 6 seven seven NUM njb-47 47 7 days day NOUN njb-47 47 8 ( ( PUNCT njb-47 47 9 168 168 NUM njb-47 47 10 h h NUM njb-47 47 11 ) ) PUNCT njb-47 47 12 of of ADP njb-47 47 13 fermentation fermentation NOUN njb-47 47 14 which which PRON njb-47 47 15 may may AUX njb-47 47 16 be be AUX njb-47 47 17 due due ADJ njb-47 47 18 to to ADP njb-47 47 19 the the DET njb-47 47 20 onset onset NOUN njb-47 47 21 of of ADP njb-47 47 22 death death NOUN njb-47 47 23 phase phase NOUN njb-47 47 24 of of ADP njb-47 47 25 microorganism microorganism NOUN njb-47 47 26 and and CCONJ njb-47 47 27 nutrient nutrient ADJ njb-47 47 28 depletion depletion NOUN njb-47 47 29 . . PUNCT njb-47 48 1 fig.1 fig.1 ADJ njb-47 48 2 : : PUNCT njb-47 48 3 effect effect NOUN njb-47 48 4 of of ADP njb-47 48 5 fermentation fermentation NOUN njb-47 48 6 time time NOUN njb-47 48 7 on on ADP njb-47 48 8 lovastatin lovastatin ADJ njb-47 48 9 production production NOUN njb-47 48 10 effect effect NOUN njb-47 48 11 of of ADP njb-47 48 12 initial initial ADJ njb-47 48 13 moisture moisture NOUN njb-47 48 14 content content NOUN njb-47 48 15 to to PART njb-47 48 16 investigate investigate VERB njb-47 48 17 the the DET njb-47 48 18 influence influence NOUN njb-47 48 19 of of ADP njb-47 48 20 initial initial ADJ njb-47 48 21 moisture moisture NOUN njb-47 48 22 content content NOUN njb-47 48 23 of of ADP njb-47 48 24 the the DET njb-47 48 25 substrate substrate NOUN njb-47 48 26 , , PUNCT njb-47 48 27 fermentation fermentation NOUN njb-47 48 28 was be AUX njb-47 48 29 carried carry VERB njb-47 48 30 out out ADP njb-47 48 31 under under ADP njb-47 48 32 various various ADJ njb-47 48 33 initial initial ADJ njb-47 48 34 moisture moisture NOUN njb-47 48 35 content content NOUN njb-47 48 36 levels level NOUN njb-47 48 37 ( ( PUNCT njb-47 48 38 40 40 NUM njb-47 48 39 , , PUNCT njb-47 48 40 50 50 NUM njb-47 48 41 , , PUNCT njb-47 48 42 60 60 NUM njb-47 48 43 , , PUNCT njb-47 48 44 70 70 NUM njb-47 48 45 , , PUNCT njb-47 48 46 80 80 NUM njb-47 48 47 , , PUNCT njb-47 48 48 90 90 NUM njb-47 48 49 and and CCONJ njb-47 48 50 100(% 100(% NUM njb-47 48 51 v v NOUN njb-47 48 52 / / SYM njb-47 48 53 w w NOUN njb-47 48 54 ) ) PUNCT njb-47 48 55 ) ) PUNCT njb-47 48 56 of of ADP njb-47 48 57 coconut coconut NOUN njb-47 48 58 oil oil NOUN njb-47 48 59 cake cake NOUN njb-47 48 60 , , PUNCT njb-47 48 61 which which PRON njb-47 48 62 was be AUX njb-47 48 63 adjusted adjust VERB njb-47 48 64 with with ADP njb-47 48 65 moistening moistening ADJ njb-47 48 66 media medium NOUN njb-47 48 67 . . PUNCT njb-47 49 1 maximum maximum PROPN njb-47 49 2 lovastatin lovastatin PROPN njb-47 49 3 yield yield NOUN njb-47 49 4 ( ( PUNCT njb-47 49 5 8.04 8.04 NUM njb-47 49 6 mg mg PROPN njb-47 49 7 / / SYM njb-47 49 8 g g NOUN njb-47 49 9 dry dry ADJ njb-47 49 10 substrate substrate NOUN njb-47 49 11 ) ) PUNCT njb-47 49 12 was be AUX njb-47 49 13 achieved achieve VERB njb-47 49 14 at at ADP njb-47 49 15 60 60 NUM njb-47 49 16 % % NOUN njb-47 49 17 ( ( PUNCT njb-47 49 18 v v NOUN njb-47 49 19 / / SYM njb-47 49 20 w w NOUN njb-47 49 21 ) ) PUNCT njb-47 49 22 initial initial ADJ njb-47 49 23 moisture moisture NOUN njb-47 49 24 content content NOUN njb-47 49 25 ( ( PUNCT njb-47 49 26 fig fig NOUN njb-47 49 27 . . PUNCT njb-47 49 28 2 2 NUM njb-47 49 29 . . PUNCT njb-47 49 30 ) ) PUNCT njb-47 49 31 . . PUNCT njb-47 50 1 as as ADP njb-47 50 2 the the DET njb-47 50 3 moisture moisture NOUN njb-47 50 4 content content NOUN njb-47 50 5 in in ADP njb-47 50 6 the the DET njb-47 50 7 fermentation fermentation NOUN njb-47 50 8 medium medium NOUN njb-47 50 9 increases increase NOUN njb-47 50 10 , , PUNCT njb-47 50 11 the the DET njb-47 50 12 air air NOUN njb-47 50 13 present present ADJ njb-47 50 14 in in ADP njb-47 50 15 the the DET njb-47 50 16 void void ADJ njb-47 50 17 volume volume NOUN njb-47 50 18 decreases decrease VERB njb-47 50 19 , , PUNCT njb-47 50 20 resulting result VERB njb-47 50 21 in in ADP njb-47 50 22 poor poor ADJ njb-47 50 23 oxygen oxygen NOUN njb-47 50 24 availability availability NOUN njb-47 50 25 for for ADP njb-47 50 26 the the DET njb-47 50 27 process process NOUN njb-47 50 28 without without ADP njb-47 50 29 forced force VERB njb-47 50 30 aeration aeration NOUN njb-47 50 31 and and CCONJ njb-47 50 32 with with ADP njb-47 50 33 low low ADJ njb-47 50 34 moisture moisture NOUN njb-47 50 35 content content NOUN njb-47 50 36 , , PUNCT njb-47 50 37 the the DET njb-47 50 38 available available ADJ njb-47 50 39 oxygen oxygen NOUN njb-47 50 40 is be AUX njb-47 50 41 sufficient sufficient ADJ njb-47 50 42 but but CCONJ njb-47 50 43 the the DET njb-47 50 44 water water NOUN njb-47 50 45 content content NOUN njb-47 50 46 is be AUX njb-47 50 47 not not PART njb-47 50 48 enough enough ADJ njb-47 50 49 to to PART njb-47 50 50 support support VERB njb-47 50 51 good good ADJ njb-47 50 52 metabolic metabolic ADJ njb-47 50 53 activity activity NOUN njb-47 50 54 and and CCONJ njb-47 50 55 dissipation dissipation NOUN njb-47 50 56 of of ADP njb-47 50 57 heat heat NOUN njb-47 50 58 generated generate VERB njb-47 50 59 and and CCONJ njb-47 50 60 may may AUX njb-47 50 61 account account VERB njb-47 50 62 for for ADP njb-47 50 63 lower low ADJ njb-47 50 64 lovastatin lovastatin ADJ njb-47 50 65 production production NOUN njb-47 50 66 . . PUNCT njb-47 51 1 the the DET njb-47 51 2 same same ADJ njb-47 51 3 60 60 NUM njb-47 51 4 % % NOUN njb-47 51 5 ( ( PUNCT njb-47 51 6 v v NOUN njb-47 51 7 / / SYM njb-47 51 8 w w NOUN njb-47 51 9 ) ) PUNCT njb-47 51 10 moisture moisture NOUN njb-47 51 11 content content NOUN njb-47 51 12 was be AUX njb-47 51 13 also also ADV njb-47 51 14 observed observe VERB njb-47 51 15 for for ADP njb-47 51 16 both both DET njb-47 51 17 aspergillus aspergillus NOUN njb-47 51 18 flavipes flavipe NOUN njb-47 51 19 and and CCONJ njb-47 51 20 aspergillus aspergillus NOUN njb-47 51 21 terreus terreus NOUN njb-47 51 22 under under ADP njb-47 51 23 ssf ssf NOUN njb-47 51 24 [ [ PUNCT njb-47 51 25 8 8 NUM njb-47 51 26 , , PUNCT njb-47 51 27 10 10 NUM njb-47 51 28 ] ] PUNCT njb-47 51 29 . . PUNCT njb-47 51 30 0 0 NUM njb-47 51 31     SPACE njb-47 51 32 1 1 NUM njb-47 51 33     SPACE njb-47 51 34 2 2 NUM njb-47 51 35     SPACE njb-47 51 36 3 3 NUM njb-47 51 37     SPACE njb-47 51 38 4 4 NUM njb-47 51 39     SPACE njb-47 51 40 5 5 NUM njb-47 51 41     SPACE njb-47 51 42 6 6 NUM njb-47 51 43     SPACE njb-47 51 44 7 7 NUM njb-47 51 45     SPACE njb-47 51 46 8 8 NUM njb-47 51 47     SPACE njb-47 51 48 24 24 NUM njb-47 51 49     SPACE njb-47 51 50 48 48 NUM njb-47 51 51     SPACE njb-47 51 52 72 72 NUM njb-47 51 53     SPACE njb-47 51 54 96 96 NUM njb-47 51 55     SPACE njb-47 51 56 120 120 NUM njb-47 51 57     SPACE njb-47 51 58 144 144 NUM njb-47 51 59     SPACE njb-47 51 60 168 168 NUM njb-47 51 61     SPACE njb-47 51 62 192 192 NUM njb-47 51 63     SPACE njb-47 52 1 y y PROPN njb-47 52 2 ie ie PROPN njb-47 52 3 ld ld PROPN njb-47 52 4 o o X njb-47 53 1 f f PROPN njb-47 53 2 l l PROPN njb-47 53 3 ov ov PROPN njb-47 53 4 as as SCONJ njb-47 53 5 ta ta PROPN njb-47 53 6 ti ti PROPN njb-47 53 7 n n NOUN njb-47 53 8 ( ( PUNCT njb-47 53 9 m m NOUN njb-47 53 10 g/ g/ PROPN njb-47 53 11 g g X njb-47 53 12 ) ) PUNCT njb-47 53 13 fementation fementation NOUN njb-47 53 14 time time NOUN njb-47 53 15 ( ( PUNCT njb-47 53 16 h h X njb-47 53 17 ) ) PUNCT njb-47 53 18 nepal nepal ADJ njb-47 53 19     SPACE njb-47 53 20 journal journal NOUN njb-47 53 21     SPACE njb-47 53 22 of of ADP njb-47 53 23     SPACE njb-47 53 24 biotechnology biotechnology NOUN njb-47 53 25 . . PUNCT njb-47 53 26         SPACE njb-47 54 1 dec dec PROPN njb-47 54 2 . . PROPN njb-47 54 3     SPACE njb-47 54 4 2011 2011 NUM njb-47 54 5 , , PUNCT njb-47 54 6     SPACE njb-47 54 7 vol vol NOUN njb-47 54 8 . . PUNCT njb-47 54 9     SPACE njb-47 54 10 2 2 NUM njb-47 54 11 , , PUNCT njb-47 54 12     SPACE njb-47 54 13 no no INTJ njb-47 54 14 . . PUNCT njb-47 54 15     SPACE njb-47 55 1 1 1 NUM njb-47 55 2 : : PUNCT njb-47 55 3     SPACE njb-47 55 4 84 84 NUM njb-47 55 5     SPACE njb-47 55 6 – – PUNCT njb-47 55 7     SPACE njb-47 55 8 xx xx NUM njb-47 55 9                                                                                                                                                                                                                                             SPACE njb-47 55 10 biotechnology biotechnology NOUN njb-47 55 11     SPACE njb-47 55 12 society society NOUN njb-47 55 13     SPACE njb-47 55 14 of of ADP njb-47 55 15     SPACE njb-47 55 16 nepal nepal NOUN njb-47 55 17     SPACE njb-47 55 18 ( ( PUNCT njb-47 55 19 bsn bsn PROPN njb-47 55 20 ) ) PUNCT njb-47 55 21 , , PUNCT njb-47 55 22     SPACE njb-47 55 23 all all DET njb-47 55 24     SPACE njb-47 55 25 rights right NOUN njb-47 55 26     SPACE njb-47 55 27 reserved reserve VERB njb-47 55 28     SPACE njb-47 55 29 . . PUNCT njb-47 56 1 fig fig NOUN njb-47 56 2 . . PUNCT njb-47 57 1 2 2 NUM njb-47 57 2 : : PUNCT njb-47 57 3 effect effect NOUN njb-47 57 4 of of ADP njb-47 57 5 moisture moisture NOUN njb-47 57 6 content content NOUN njb-47 57 7 on on ADP njb-47 57 8 lovastatin lovastatin ADJ njb-47 57 9 production production NOUN njb-47 57 10 effect effect NOUN njb-47 57 11 of of ADP njb-47 57 12 inoculum inoculum PROPN njb-47 57 13 volume volume NOUN njb-47 57 14 fermentation fermentation NOUN njb-47 57 15 was be AUX njb-47 57 16 carried carry VERB njb-47 57 17 out out ADP njb-47 57 18 with with ADP njb-47 57 19 different different ADJ njb-47 57 20 inoculum inoculum PROPN njb-47 57 21 volumes volume NOUN njb-47 57 22 ( ( PUNCT njb-47 57 23 0.5 0.5 NUM njb-47 57 24 - - PUNCT njb-47 57 25 4ml 4ml NOUN njb-47 57 26 ) ) PUNCT njb-47 57 27 of of ADP njb-47 57 28 five five NUM njb-47 57 29 day day NOUN njb-47 57 30 old old ADJ njb-47 57 31 a. a. NOUN njb-47 57 32 fischeri fischeri PROPN njb-47 57 33 culture culture NOUN njb-47 57 34 for for ADP njb-47 57 35 a a DET njb-47 57 36 period period NOUN njb-47 57 37 of of ADP njb-47 57 38 144h 144h PROPN njb-47 57 39 to to PART njb-47 57 40 study study VERB njb-47 57 41 its its PRON njb-47 57 42 effect effect NOUN njb-47 57 43 on on ADP njb-47 57 44 lovastatin lovastatin ADJ njb-47 57 45 production production NOUN njb-47 57 46 . . PUNCT njb-47 58 1 the the DET njb-47 58 2 maximum maximum ADJ njb-47 58 3 yield yield NOUN njb-47 58 4 of of ADP njb-47 58 5 lovastation lovastation NOUN njb-47 58 6 ( ( PUNCT njb-47 58 7 8.97 8.97 NUM njb-47 58 8 mg mg NUM njb-47 58 9 / / SYM njb-47 58 10 g g NOUN njb-47 58 11 dry dry ADJ njb-47 58 12 substrate substrate NOUN njb-47 58 13 ) ) PUNCT njb-47 58 14 was be AUX njb-47 58 15 obtained obtain VERB njb-47 58 16 with with ADP njb-47 58 17 2ml 2ml NOUN njb-47 58 18 of of ADP njb-47 58 19 a.fischeri a.fischeri ADJ njb-47 58 20 culture culture NOUN njb-47 58 21 as as SCONJ njb-47 58 22 shown show VERB njb-47 58 23 in in ADP njb-47 58 24 fig fig PROPN njb-47 58 25 . . PUNCT njb-47 59 1 3 3 X njb-47 59 2 . . X njb-47 59 3 with with ADP njb-47 59 4 the the DET njb-47 59 5 further further ADJ njb-47 59 6 increase increase NOUN njb-47 59 7 in in ADP njb-47 59 8 inoculum inoculum PROPN njb-47 59 9 volume volume NOUN njb-47 59 10 , , PUNCT njb-47 59 11 the the DET njb-47 59 12 yield yield NOUN njb-47 59 13 of of ADP njb-47 59 14 lovastatin lovastatin PROPN njb-47 59 15 had have AUX njb-47 59 16 decreased decrease VERB njb-47 59 17 which which PRON njb-47 59 18 might might AUX njb-47 59 19 be be AUX njb-47 59 20 due due ADJ njb-47 59 21 to to ADP njb-47 59 22 the the DET njb-47 59 23 depletion depletion NOUN njb-47 59 24 of of ADP njb-47 59 25 the the DET njb-47 59 26 available available ADJ njb-47 59 27 nutrients nutrient NOUN njb-47 59 28 in in ADP njb-47 59 29 the the DET njb-47 59 30 production production NOUN njb-47 59 31 medium medium NOUN njb-47 59 32 , , PUNCT njb-47 59 33 yielding yield VERB njb-47 59 34 poor poor ADJ njb-47 59 35 mycelia mycelia NOUN njb-47 59 36 growth growth NOUN njb-47 59 37 , , PUNCT njb-47 59 38 thus thus ADV njb-47 59 39 promoting promote VERB njb-47 59 40 less less ADJ njb-47 59 41 product product NOUN njb-47 59 42 formation formation NOUN njb-47 59 43 . . PUNCT njb-47 60 1 with with ADP njb-47 60 2 low low ADJ njb-47 60 3 inoculum inoculum PROPN njb-47 60 4 volume volume NOUN njb-47 60 5 , , PUNCT njb-47 60 6 the the DET njb-47 60 7 yield yield NOUN njb-47 60 8 is be AUX njb-47 60 9 also also ADV njb-47 60 10 low low ADJ njb-47 60 11 due due ADP njb-47 60 12 to to ADP njb-47 60 13 the the DET njb-47 60 14 insufficient insufficient ADJ njb-47 60 15 microbial microbial ADJ njb-47 60 16 culture culture NOUN njb-47 60 17 to to PART njb-47 60 18 form form VERB njb-47 60 19 mycelia mycelia NOUN njb-47 60 20 and and CCONJ njb-47 60 21 produce produce VERB njb-47 60 22 lovastatin lovastatin PROPN njb-47 60 23 . . PUNCT njb-47 61 1 0 0 NUM njb-47 61 2     SPACE njb-47 61 3 1 1 NUM njb-47 61 4     SPACE njb-47 61 5 2 2 NUM njb-47 61 6     SPACE njb-47 61 7 3 3 NUM njb-47 61 8     SPACE njb-47 61 9 4 4 NUM njb-47 61 10     SPACE njb-47 61 11 5 5 NUM njb-47 61 12     SPACE njb-47 61 13 6 6 NUM njb-47 61 14     SPACE njb-47 61 15 7 7 NUM njb-47 61 16     SPACE njb-47 61 17 8 8 NUM njb-47 61 18     SPACE njb-47 61 19 9 9 NUM njb-47 61 20     SPACE njb-47 61 21 40 40 NUM njb-47 61 22     SPACE njb-47 61 23 50 50 NUM njb-47 61 24     SPACE njb-47 61 25 60 60 NUM njb-47 61 26     SPACE njb-47 61 27 70 70 NUM njb-47 61 28     SPACE njb-47 61 29 80 80 NUM njb-47 61 30     SPACE njb-47 61 31 90 90 NUM njb-47 61 32     SPACE njb-47 61 33 100 100 NUM njb-47 61 34     SPACE njb-47 61 35 y y PROPN njb-47 61 36 ie ie X njb-47 61 37 ld ld PROPN njb-47 61 38 o o X njb-47 62 1 f f PROPN njb-47 62 2 l l PROPN njb-47 62 3 ov ov PROPN njb-47 62 4 as as SCONJ njb-47 62 5 ta ta PROPN njb-47 62 6 ti ti PROPN njb-47 62 7 n n NOUN njb-47 62 8 ( ( PUNCT njb-47 62 9 m m NOUN njb-47 62 10 g/ g/ PROPN njb-47 62 11 g g X njb-47 62 12 ) ) PUNCT njb-47 62 13 moisture moisture NOUN njb-47 62 14 content content NOUN njb-47 62 15 ( ( PUNCT njb-47 62 16 % % NOUN njb-47 62 17 v v NOUN njb-47 62 18 / / SYM njb-47 62 19 w w PROPN njb-47 62 20 ) ) PUNCT njb-47 62 21 nepal nepal ADJ njb-47 62 22     SPACE njb-47 62 23 journal journal NOUN njb-47 62 24     SPACE njb-47 62 25 of of ADP njb-47 62 26     SPACE njb-47 62 27 biotechnology biotechnology NOUN njb-47 62 28 . . PUNCT njb-47 62 29         SPACE njb-47 63 1 dec dec PROPN njb-47 63 2 . . PROPN njb-47 63 3     SPACE njb-47 63 4 2011 2011 NUM njb-47 63 5 , , PUNCT njb-47 63 6     SPACE njb-47 63 7 vol vol NOUN njb-47 63 8 . . PUNCT njb-47 63 9     SPACE njb-47 63 10 2 2 NUM njb-47 63 11 , , PUNCT njb-47 63 12     SPACE njb-47 63 13 no no INTJ njb-47 63 14 . . PUNCT njb-47 63 15     SPACE njb-47 64 1 1 1 NUM njb-47 64 2 : : PUNCT njb-47 64 3     SPACE njb-47 64 4 84 84 NUM njb-47 64 5     SPACE njb-47 64 6 – – PUNCT njb-47 64 7     SPACE njb-47 64 8 xx xx NUM njb-47 64 9                                                                                                                                                                                                                                             SPACE njb-47 64 10 biotechnology biotechnology NOUN njb-47 64 11     SPACE njb-47 64 12 society society NOUN njb-47 64 13     SPACE njb-47 64 14 of of ADP njb-47 64 15     SPACE njb-47 64 16 nepal nepal NOUN njb-47 64 17     SPACE njb-47 64 18 ( ( PUNCT njb-47 64 19 bsn bsn PROPN njb-47 64 20 ) ) PUNCT njb-47 64 21 , , PUNCT njb-47 64 22     SPACE njb-47 64 23 all all DET njb-47 64 24     SPACE njb-47 64 25 rights right NOUN njb-47 64 26     SPACE njb-47 64 27 reserved reserve VERB njb-47 64 28     SPACE njb-47 64 29 . . PUNCT njb-47 65 1 fig fig NOUN njb-47 65 2 . . PUNCT njb-47 66 1 3 3 NUM njb-47 66 2 : : PUNCT njb-47 66 3 effect effect NOUN njb-47 66 4 of of ADP njb-47 66 5 inoculum inoculum PROPN njb-47 66 6 volume volume NOUN njb-47 66 7 on on ADP njb-47 66 8 lovastatin lovastatin ADJ njb-47 66 9 production production NOUN njb-47 66 10 effect effect NOUN njb-47 66 11 of of ADP njb-47 66 12 initial initial ADJ njb-47 66 13 ph ph NOUN njb-47 66 14 : : PUNCT njb-47 66 15 experiments experiment NOUN njb-47 66 16 were be AUX njb-47 66 17 performed perform VERB njb-47 66 18 to to ADP njb-47 66 19 the the DET njb-47 66 20 optimum optimum ADJ njb-47 66 21 ph ph NOUN njb-47 66 22 in in ADP njb-47 66 23 order order NOUN njb-47 66 24 to to PART njb-47 66 25 maintain maintain VERB njb-47 66 26 the the DET njb-47 66 27 favorable favorable ADJ njb-47 66 28 conditions condition NOUN njb-47 66 29 for for ADP njb-47 66 30 increased increase VERB njb-47 66 31 production production NOUN njb-47 66 32 of of ADP njb-47 66 33 lovastatin lovastatin PROPN njb-47 66 34 . . PUNCT njb-47 67 1 this this PRON njb-47 67 2 was be AUX njb-47 67 3 established establish VERB njb-47 67 4 by by ADP njb-47 67 5 carrying carry VERB njb-47 67 6 out out ADP njb-47 67 7 the the DET njb-47 67 8 fermentation fermentation NOUN njb-47 67 9 by by ADP njb-47 67 10 varying vary VERB njb-47 67 11 the the DET njb-47 67 12 ph ph NOUN njb-47 67 13 from from ADP njb-47 67 14 4 4 NUM njb-47 67 15 - - SYM njb-47 67 16 10 10 NUM njb-47 67 17 . . PUNCT njb-47 68 1 the the DET njb-47 68 2 profound profound ADJ njb-47 68 3 effect effect NOUN njb-47 68 4 of of ADP njb-47 68 5 initial initial ADJ njb-47 68 6 ph ph NOUN njb-47 68 7 of of ADP njb-47 68 8 the the DET njb-47 68 9 fermentation fermentation NOUN njb-47 68 10 on on ADP njb-47 68 11 lovastatin lovastatin ADJ njb-47 68 12 production production NOUN njb-47 68 13 was be AUX njb-47 68 14 as as SCONJ njb-47 68 15 shown show VERB njb-47 68 16 in in ADP njb-47 68 17 fig fig PROPN njb-47 68 18 . . PUNCT njb-47 69 1 4 4 X njb-47 69 2 . . X njb-47 69 3 maximum maximum PROPN njb-47 69 4 lovastatin lovastatin PROPN njb-47 69 5 yield yield NOUN njb-47 69 6 ( ( PUNCT njb-47 69 7 9.71 9.71 NUM njb-47 69 8 mg mg PROPN njb-47 69 9 / / SYM njb-47 69 10 g g NOUN njb-47 69 11 dry dry ADJ njb-47 69 12 substrate substrate NOUN njb-47 69 13 ) ) PUNCT njb-47 69 14 was be AUX njb-47 69 15 recorded record VERB njb-47 69 16 at at ADP njb-47 69 17 ph ph NOUN njb-47 69 18 5.0 5.0 NUM njb-47 69 19 . . PUNCT njb-47 70 1 a a DET njb-47 70 2 further further ADJ njb-47 70 3 increase increase NOUN njb-47 70 4 in in ADP njb-47 70 5 ph ph NOUN njb-47 70 6 resulted result VERB njb-47 70 7 in in ADP njb-47 70 8 gradual gradual ADJ njb-47 70 9 decrease decrease NOUN njb-47 70 10 of of ADP njb-47 70 11 lovastatin lovastatin ADJ njb-47 70 12 production production NOUN njb-47 70 13 due due ADP njb-47 70 14 to to ADP njb-47 70 15 the the DET njb-47 70 16 denaturation denaturation NOUN njb-47 70 17 or or CCONJ njb-47 70 18 inactivation inactivation NOUN njb-47 70 19 of of ADP njb-47 70 20 the the DET njb-47 70 21 microbial microbial ADJ njb-47 70 22 strain strain NOUN njb-47 70 23 , , PUNCT njb-47 70 24 because because SCONJ njb-47 70 25 ph ph NOUN njb-47 70 26 strongly strongly ADV njb-47 70 27 influences influence VERB njb-47 70 28 the the DET njb-47 70 29 transport transport NOUN njb-47 70 30 of of ADP njb-47 70 31 various various ADJ njb-47 70 32 components component NOUN njb-47 70 33 across across ADP njb-47 70 34 the the DET njb-47 70 35 cell cell NOUN njb-47 70 36 membrane membrane NOUN njb-47 70 37 which which PRON njb-47 70 38 support support VERB njb-47 70 39 the the DET njb-47 70 40 cell cell NOUN njb-47 70 41 growth growth NOUN njb-47 70 42 and and CCONJ njb-47 70 43 product product NOUN njb-47 70 44 formation formation NOUN njb-47 70 45 , , PUNCT njb-47 70 46 and and CCONJ njb-47 70 47 most most ADJ njb-47 70 48 of of ADP njb-47 70 49 the the DET njb-47 70 50 fungi fungi PROPN njb-47 70 51 are be AUX njb-47 70 52 active active ADJ njb-47 70 53 in in ADP njb-47 70 54 the the DET njb-47 70 55 ph ph NOUN njb-47 70 56 range range NOUN njb-47 70 57 of of ADP njb-47 70 58 3.5 3.5 NUM njb-47 70 59 - - SYM njb-47 70 60 7 7 NUM njb-47 70 61 and and CCONJ njb-47 70 62 also also ADV njb-47 70 63 lower low ADJ njb-47 70 64 ph ph NOUN njb-47 70 65 avoids avoid VERB njb-47 70 66 the the DET njb-47 70 67 contamination contamination NOUN njb-47 70 68 by by ADP njb-47 70 69 other other ADJ njb-47 70 70 microbes microbe NOUN njb-47 70 71 . . PUNCT njb-47 71 1 the the DET njb-47 71 2 result result NOUN njb-47 71 3 was be AUX njb-47 71 4 in in ADP njb-47 71 5 accordance accordance NOUN njb-47 71 6 with with ADP njb-47 71 7 the the DET njb-47 71 8 lovastation lovastation NOUN njb-47 71 9 production production NOUN njb-47 71 10 using use VERB njb-47 71 11 aspergillus aspergillus NOUN njb-47 71 12 terreus terreus NOUN njb-47 71 13 under under ADP njb-47 71 14 ssf ssf PROPN njb-47 71 15 [ [ PUNCT njb-47 71 16 8 8 NUM njb-47 71 17 ] ] PUNCT njb-47 71 18 . . PUNCT njb-47 71 19 0 0 NUM njb-47 71 20 1 1 NUM njb-47 71 21 2 2 NUM njb-47 71 22 3 3 NUM njb-47 71 23 4 4 NUM njb-47 71 24 5 5 NUM njb-47 71 25 6 6 NUM njb-47 71 26 7 7 NUM njb-47 71 27 8 8 NUM njb-47 71 28 9 9 NUM njb-47 71 29 10 10 NUM njb-47 71 30 0.5 0.5 NUM njb-47 71 31 1 1 NUM njb-47 71 32 1.5 1.5 NUM njb-47 71 33 2 2 NUM njb-47 71 34 2.5 2.5 NUM njb-47 71 35 3 3 NUM njb-47 71 36 3.5 3.5 NUM njb-47 71 37 4 4 NUM njb-47 71 38 y y PROPN njb-47 71 39 ie ie X njb-47 71 40 ld ld PROPN njb-47 71 41 o o X njb-47 72 1 f f PROPN njb-47 72 2 l l PROPN njb-47 72 3 ov ov PROPN njb-47 72 4 as as SCONJ njb-47 72 5 ta ta PROPN njb-47 72 6 ti ti PROPN njb-47 72 7 n n NOUN njb-47 72 8 ( ( PUNCT njb-47 72 9 m m NOUN njb-47 72 10 g/ g/ PROPN njb-47 72 11 g g X njb-47 72 12 ) ) PUNCT njb-47 72 13 inoculum inoculum PROPN njb-47 72 14 volume volume NOUN njb-47 72 15 ( ( PUNCT njb-47 72 16 ml ml PROPN njb-47 72 17 ) ) PUNCT njb-47 72 18 nepal nepal ADJ njb-47 72 19     SPACE njb-47 72 20 journal journal NOUN njb-47 72 21     SPACE njb-47 72 22 of of ADP njb-47 72 23     SPACE njb-47 72 24 biotechnology biotechnology NOUN njb-47 72 25 . . PUNCT njb-47 72 26         SPACE njb-47 73 1 dec dec PROPN njb-47 73 2 . . PROPN njb-47 73 3     SPACE njb-47 73 4 2011 2011 NUM njb-47 73 5 , , PUNCT njb-47 73 6     SPACE njb-47 73 7 vol vol NOUN njb-47 73 8 . . PUNCT njb-47 73 9     SPACE njb-47 73 10 2 2 NUM njb-47 73 11 , , PUNCT njb-47 73 12     SPACE njb-47 73 13 no no INTJ njb-47 73 14 . . PUNCT njb-47 73 15     SPACE njb-47 74 1 1 1 NUM njb-47 74 2 : : PUNCT njb-47 74 3     SPACE njb-47 74 4 84 84 NUM njb-47 74 5     SPACE njb-47 74 6 – – PUNCT njb-47 74 7     SPACE njb-47 74 8 xx xx NUM njb-47 74 9                                                                                                                                                                                                                                             SPACE njb-47 74 10 biotechnology biotechnology NOUN njb-47 74 11     SPACE njb-47 74 12 society society NOUN njb-47 74 13     SPACE njb-47 74 14 of of ADP njb-47 74 15     SPACE njb-47 74 16 nepal nepal NOUN njb-47 74 17     SPACE njb-47 74 18 ( ( PUNCT njb-47 74 19 bsn bsn PROPN njb-47 74 20 ) ) PUNCT njb-47 74 21 , , PUNCT njb-47 74 22     SPACE njb-47 74 23 all all DET njb-47 74 24     SPACE njb-47 74 25 rights right NOUN njb-47 74 26     SPACE njb-47 74 27 reserved reserve VERB njb-47 74 28     SPACE njb-47 74 29 . . PUNCT njb-47 75 1 fig fig NOUN njb-47 75 2 . . PUNCT njb-47 76 1 4 4 NUM njb-47 76 2 : : PUNCT njb-47 76 3 effect effect NOUN njb-47 76 4 of of ADP njb-47 76 5 ph ph NOUN njb-47 76 6 on on ADP njb-47 76 7 lovastatin lovastatin ADJ njb-47 76 8 production production NOUN njb-47 76 9 . . PUNCT njb-47 77 1 effect effect NOUN njb-47 77 2 of of ADP njb-47 77 3 temperature temperature NOUN njb-47 77 4 fermentation fermentation NOUN njb-47 77 5 was be AUX njb-47 77 6 carried carry VERB njb-47 77 7 out out ADP njb-47 77 8 at at ADP njb-47 77 9 various various ADJ njb-47 77 10 temperatures temperature NOUN njb-47 77 11 from from ADP njb-47 77 12 26 26 NUM njb-47 77 13 - - SYM njb-47 77 14 40 40 NUM njb-47 77 15 ° ° NOUN njb-47 77 16 c c NOUN njb-47 77 17 to to PART njb-47 77 18 study study VERB njb-47 77 19 their their PRON njb-47 77 20 effect effect NOUN njb-47 77 21 on on ADP njb-47 77 22 enzyme enzyme NOUN njb-47 77 23 production production NOUN njb-47 77 24 ( ( PUNCT njb-47 77 25 fig fig NOUN njb-47 77 26 . . PROPN njb-47 77 27 5 5 NUM njb-47 77 28 ) ) PUNCT njb-47 77 29 . . PUNCT njb-47 78 1 results result NOUN njb-47 78 2 indicated indicate VERB njb-47 78 3 that that SCONJ njb-47 78 4 maximum maximum ADJ njb-47 78 5 lovastatin lovastatin ADJ njb-47 78 6 production production NOUN njb-47 78 7 ( ( PUNCT njb-47 78 8 10.88 10.88 NUM njb-47 78 9 mg mg NUM njb-47 78 10 / / SYM njb-47 78 11 g g NOUN njb-47 78 12 dry dry ADJ njb-47 78 13 substrate substrate NOUN njb-47 78 14 ) ) PUNCT njb-47 78 15 was be AUX njb-47 78 16 obtained obtain VERB njb-47 78 17 when when SCONJ njb-47 78 18 ssf ssf PROPN njb-47 78 19 was be AUX njb-47 78 20 carried carry VERB njb-47 78 21 out out ADP njb-47 78 22 at at ADP njb-47 78 23 30 30 NUM njb-47 78 24 ° ° NUM njb-47 78 25 c c NOUN njb-47 78 26 . . PUNCT njb-47 79 1 however however ADV njb-47 79 2 , , PUNCT njb-47 79 3 lovastatin lovastatin PROPN njb-47 79 4 production production NOUN njb-47 79 5 reduced reduce VERB njb-47 79 6 gradually gradually ADV njb-47 79 7 above above ADP njb-47 79 8 the the DET njb-47 79 9 optimal optimal ADJ njb-47 79 10 incubation incubation NOUN njb-47 79 11 temperature temperature NOUN njb-47 79 12 of of ADP njb-47 79 13 30 30 NUM njb-47 79 14 ° ° NUM njb-47 79 15 c c NOUN njb-47 79 16 . . PUNCT njb-47 80 1 with with ADP njb-47 80 2 further further ADJ njb-47 80 3 increase increase NOUN njb-47 80 4 in in ADP njb-47 80 5 temperature temperature NOUN njb-47 80 6 , , PUNCT njb-47 80 7 more more ADJ njb-47 80 8 heat heat NOUN njb-47 80 9 is be AUX njb-47 80 10 accumulated accumulate VERB njb-47 80 11 in in ADP njb-47 80 12 the the DET njb-47 80 13 medium medium NOUN njb-47 80 14 during during ADP njb-47 80 15 mesophilic mesophilic ADJ njb-47 80 16 aerobic aerobic ADJ njb-47 80 17 ssf ssf NOUN njb-47 80 18 , , PUNCT njb-47 80 19 which which PRON njb-47 80 20 leads lead VERB njb-47 80 21 to to ADP njb-47 80 22 poor poor ADJ njb-47 80 23 heat heat NOUN njb-47 80 24 dissipation dissipation NOUN njb-47 80 25 thus thus ADV njb-47 80 26 reducing reduce VERB njb-47 80 27 the the DET njb-47 80 28 oxygen oxygen NOUN njb-47 80 29 level level NOUN njb-47 80 30 and and CCONJ njb-47 80 31 thereby thereby ADV njb-47 80 32 reducing reduce VERB njb-47 80 33 the the DET njb-47 80 34 growth growth NOUN njb-47 80 35 of of ADP njb-47 80 36 microorganism microorganism NOUN njb-47 80 37 , , PUNCT njb-47 80 38 as as SCONJ njb-47 80 39 lovastatin lovastatin PROPN njb-47 80 40 is be AUX njb-47 80 41 growth growth NOUN njb-47 80 42 related related ADJ njb-47 80 43 product product NOUN njb-47 80 44 . . PUNCT njb-47 81 1 these these DET njb-47 81 2 results result NOUN njb-47 81 3 are be AUX njb-47 81 4 coinciding coincide VERB njb-47 81 5 with with ADP njb-47 81 6 those those PRON njb-47 81 7 previously previously ADV njb-47 81 8 reported report VERB njb-47 81 9 for for ADP njb-47 81 10 lovastatin lovastatin ADJ njb-47 81 11 production production NOUN njb-47 81 12 by by ADP njb-47 81 13 aspergillus aspergillus NOUN njb-47 81 14 terreus terreus NOUN njb-47 81 15 and and CCONJ njb-47 81 16 monascus monascus ADJ njb-47 81 17 ruber ruber PROPN njb-47 82 1 [ [ X njb-47 82 2 10,13 10,13 NUM njb-47 82 3 ] ] SYM njb-47 82 4 . . PUNCT njb-47 82 5 0 0 NUM njb-47 82 6     SPACE njb-47 82 7 2 2 NUM njb-47 82 8     SPACE njb-47 82 9 4 4 NUM njb-47 82 10     SPACE njb-47 82 11 6 6 NUM njb-47 82 12     SPACE njb-47 82 13 8 8 NUM njb-47 82 14     SPACE njb-47 82 15 10 10 NUM njb-47 82 16     SPACE njb-47 82 17 12 12 NUM njb-47 82 18     SPACE njb-47 82 19 4 4 NUM njb-47 82 20     SPACE njb-47 82 21 5 5 NUM njb-47 82 22     SPACE njb-47 82 23 6 6 NUM njb-47 82 24     SPACE njb-47 82 25 7 7 NUM njb-47 82 26     SPACE njb-47 82 27 8 8 NUM njb-47 82 28     SPACE njb-47 82 29 9 9 NUM njb-47 82 30     SPACE njb-47 82 31 10 10 NUM njb-47 82 32     SPACE njb-47 82 33 y y PROPN njb-47 82 34 ie ie X njb-47 82 35 ld ld PROPN njb-47 82 36 o o X njb-47 83 1 f f PROPN njb-47 83 2 l l PROPN njb-47 83 3 ov ov PROPN njb-47 83 4 as as SCONJ njb-47 83 5 ta ta PROPN njb-47 83 6 ti ti PROPN njb-47 83 7 n n NOUN njb-47 83 8 ( ( PUNCT njb-47 83 9 m m NOUN njb-47 83 10 g/ g/ PROPN njb-47 83 11 g g X njb-47 83 12 ) ) PUNCT njb-47 83 13 ph ph PROPN njb-47 83 14 nepal nepal ADJ njb-47 83 15     SPACE njb-47 83 16 journal journal NOUN njb-47 83 17     SPACE njb-47 83 18 of of ADP njb-47 83 19     SPACE njb-47 83 20 biotechnology biotechnology NOUN njb-47 83 21 . . PUNCT njb-47 83 22         SPACE njb-47 84 1 dec dec PROPN njb-47 84 2 . . PROPN njb-47 84 3     SPACE njb-47 84 4 2011 2011 NUM njb-47 84 5 , , PUNCT njb-47 84 6     SPACE njb-47 84 7 vol vol NOUN njb-47 84 8 . . PUNCT njb-47 84 9     SPACE njb-47 84 10 2 2 NUM njb-47 84 11 , , PUNCT njb-47 84 12     SPACE njb-47 84 13 no no INTJ njb-47 84 14 . . PUNCT njb-47 84 15     SPACE njb-47 85 1 1 1 NUM njb-47 85 2 : : PUNCT njb-47 85 3     SPACE njb-47 85 4 84 84 NUM njb-47 85 5     SPACE njb-47 85 6 – – PUNCT njb-47 85 7     SPACE njb-47 85 8 xx xx NUM njb-47 85 9                                                                                                                                                                                                                                             SPACE njb-47 85 10 biotechnology biotechnology NOUN njb-47 85 11     SPACE njb-47 85 12 society society NOUN njb-47 85 13     SPACE njb-47 85 14 of of ADP njb-47 85 15     SPACE njb-47 85 16 nepal nepal NOUN njb-47 85 17     SPACE njb-47 85 18 ( ( PUNCT njb-47 85 19 bsn bsn PROPN njb-47 85 20 ) ) PUNCT njb-47 85 21 , , PUNCT njb-47 85 22     SPACE njb-47 85 23 all all DET njb-47 85 24     SPACE njb-47 85 25 rights right NOUN njb-47 85 26     SPACE njb-47 85 27 reserved reserve VERB njb-47 85 28     SPACE njb-47 85 29 . . PUNCT njb-47 86 1 fig fig NOUN njb-47 86 2 . . PUNCT njb-47 87 1 5 5 NUM njb-47 87 2 : : PUNCT njb-47 87 3 effect effect NOUN njb-47 87 4 of of ADP njb-47 87 5 temperature temperature NOUN njb-47 87 6 on on ADP njb-47 87 7 lovastatin lovastatin ADJ njb-47 87 8 production production NOUN njb-47 87 9 effect effect NOUN njb-47 87 10 of of ADP njb-47 87 11 carbon carbon NOUN njb-47 87 12 source source NOUN njb-47 87 13 to to PART njb-47 87 14 determine determine VERB njb-47 87 15 the the DET njb-47 87 16 effect effect NOUN njb-47 87 17 of of ADP njb-47 87 18 incorporation incorporation NOUN njb-47 87 19 of of ADP njb-47 87 20 additional additional ADJ njb-47 87 21 carbon carbon NOUN njb-47 87 22 sources source NOUN njb-47 87 23 on on ADP njb-47 87 24 lovastatin lovastatin PROPN njb-47 87 25 yield yield NOUN njb-47 87 26 , , PUNCT njb-47 87 27 different different ADJ njb-47 87 28 carbon carbon NOUN njb-47 87 29 sources source NOUN njb-47 87 30 namely namely ADV njb-47 87 31 glucose glucose NOUN njb-47 87 32 , , PUNCT njb-47 87 33 maltose maltose NOUN njb-47 87 34 , , PUNCT njb-47 87 35 sucrose sucrose NOUN njb-47 87 36 , , PUNCT njb-47 87 37 xylose xylose VERB njb-47 87 38 , , PUNCT njb-47 87 39 lactose lactose NOUN njb-47 87 40 , , PUNCT njb-47 87 41 galactose galactose NOUN njb-47 87 42 and and CCONJ njb-47 87 43 soluble soluble ADJ njb-47 87 44 starch starch NOUN njb-47 87 45 at at ADP njb-47 87 46 1 1 NUM njb-47 87 47 % % NOUN njb-47 87 48 ( ( PUNCT njb-47 87 49 w w PROPN njb-47 87 50 / / SYM njb-47 87 51 v v NOUN njb-47 87 52 ) ) PUNCT njb-47 87 53 added add VERB njb-47 87 54 to to ADP njb-47 87 55 the the DET njb-47 87 56 basal basal ADJ njb-47 87 57 solid solid ADJ njb-47 87 58 state state NOUN njb-47 87 59 fermentative fermentative ADJ njb-47 87 60 medium medium NOUN njb-47 87 61 of of ADP njb-47 87 62 a. a. PROPN njb-47 87 63 fischeri fischeri PROPN njb-47 87 64 exerted exert VERB njb-47 87 65 a a DET njb-47 87 66 considerable considerable ADJ njb-47 87 67 effect effect NOUN njb-47 87 68 on on ADP njb-47 87 69 the the DET njb-47 87 70 biosynthesis biosynthesis NOUN njb-47 87 71 of of ADP njb-47 87 72 lovastatin lovastatin PROPN njb-47 87 73 ( ( PUNCT njb-47 87 74 fig fig NOUN njb-47 87 75 . . PUNCT njb-47 87 76 6 6 NUM njb-47 87 77 ) ) PUNCT njb-47 87 78 . . PUNCT njb-47 88 1 maximum maximum PROPN njb-47 88 2 lovastatin lovastatin ADJ njb-47 88 3 production production NOUN njb-47 88 4 was be AUX njb-47 88 5 promoted promote VERB njb-47 88 6 by by ADP njb-47 88 7 lactose lactose NOUN njb-47 88 8 ( ( PUNCT njb-47 88 9 12.02 12.02 NUM njb-47 88 10 mg mg PROPN njb-47 88 11 / / SYM njb-47 88 12 g g NOUN njb-47 88 13 dry dry ADJ njb-47 88 14 substrate substrate NOUN njb-47 88 15 ) ) PUNCT njb-47 88 16 followed follow VERB njb-47 88 17 by by ADP njb-47 88 18 maltose maltose PROPN njb-47 88 19 ( ( PUNCT njb-47 88 20 11.11 11.11 NUM njb-47 88 21 mg mg PROPN njb-47 88 22 / / SYM njb-47 88 23 g g NOUN njb-47 88 24 dry dry ADJ njb-47 88 25 substrate substrate NOUN njb-47 88 26 ) ) PUNCT njb-47 88 27 and and CCONJ njb-47 88 28 sucrose sucrose NOUN njb-47 88 29 ( ( PUNCT njb-47 88 30 10.47 10.47 NUM njb-47 88 31 mg mg PROPN njb-47 88 32 / / SYM njb-47 88 33 g g NOUN njb-47 88 34 dry dry ADJ njb-47 88 35 substrate substrate NOUN njb-47 88 36 ) ) PUNCT njb-47 88 37 . . PUNCT njb-47 89 1 the the DET njb-47 89 2 enhanced enhance VERB njb-47 89 3 production production NOUN njb-47 89 4 of of ADP njb-47 89 5 lovastatin lovastatin PROPN njb-47 89 6 by by ADP njb-47 89 7 the the DET njb-47 89 8 incorporation incorporation NOUN njb-47 89 9 of of ADP njb-47 89 10 lactose lactose NOUN njb-47 89 11 to to ADP njb-47 89 12 the the DET njb-47 89 13 medium medium NOUN njb-47 89 14 might might AUX njb-47 89 15 be be AUX njb-47 89 16 attributed attribute VERB njb-47 89 17 to to ADP njb-47 89 18 the the DET njb-47 89 19 positive positive ADJ njb-47 89 20 influence influence NOUN njb-47 89 21 of of ADP njb-47 89 22 lactose lactose NOUN njb-47 89 23 as as ADP njb-47 89 24 a a DET njb-47 89 25 cometabolic cometabolic ADJ njb-47 89 26 agent agent NOUN njb-47 89 27 for for ADP njb-47 89 28 enhanced enhance VERB njb-47 89 29 microbial microbial ADJ njb-47 89 30 metabolite metabolite NOUN njb-47 89 31 biosynthesis biosynthesis NOUN njb-47 89 32 . . PUNCT njb-47 90 1 fig fig NOUN njb-47 90 2 . . PUNCT njb-47 91 1 6 6 NUM njb-47 91 2 : : PUNCT njb-47 91 3 effect effect NOUN njb-47 91 4 of of ADP njb-47 91 5 different different ADJ njb-47 91 6 carbon carbon NOUN njb-47 91 7 sources source NOUN njb-47 91 8 on on ADP njb-47 91 9 lovastatin lovastatin ADJ njb-47 91 10 production production NOUN njb-47 91 11 . . PUNCT njb-47 92 1 0 0 PUNCT njb-47 92 2     SPACE njb-47 92 3 2 2 NUM njb-47 92 4     SPACE njb-47 92 5 4 4 NUM njb-47 92 6     SPACE njb-47 92 7 6 6 NUM njb-47 92 8     SPACE njb-47 92 9 8 8 NUM njb-47 92 10     SPACE njb-47 92 11 10 10 NUM njb-47 92 12     SPACE njb-47 92 13 12 12 NUM njb-47 92 14     SPACE njb-47 92 15 26 26 NUM njb-47 92 16     SPACE njb-47 92 17 28 28 NUM njb-47 92 18     SPACE njb-47 92 19 30 30 NUM njb-47 92 20     SPACE njb-47 92 21 32 32 NUM njb-47 92 22     SPACE njb-47 92 23 34 34 NUM njb-47 92 24     SPACE njb-47 92 25 36 36 NUM njb-47 92 26     SPACE njb-47 92 27 38 38 NUM njb-47 92 28     SPACE njb-47 92 29 40 40 NUM njb-47 92 30     SPACE njb-47 92 31 y y PROPN njb-47 92 32 ie ie X njb-47 92 33 ld ld PROPN njb-47 92 34 o o X njb-47 93 1 f f PROPN njb-47 93 2 l l PROPN njb-47 93 3 ov ov PROPN njb-47 93 4 as as SCONJ njb-47 93 5 ta ta PROPN njb-47 93 6 ti ti PROPN njb-47 93 7 n n NOUN njb-47 93 8 ( ( PUNCT njb-47 93 9 m m NOUN njb-47 93 10 g/ g/ PROPN njb-47 93 11 g g X njb-47 93 12 ) ) PUNCT njb-47 93 13 temperature temperature NOUN njb-47 93 14 ( ( PUNCT njb-47 93 15 ˚c ˚c PROPN njb-47 93 16 ) ) PUNCT njb-47 93 17 0 0 NUM njb-47 93 18     SPACE njb-47 93 19 2 2 NUM njb-47 93 20     SPACE njb-47 93 21 4 4 NUM njb-47 93 22     SPACE njb-47 93 23 6 6 NUM njb-47 93 24     SPACE njb-47 93 25 8 8 NUM njb-47 93 26     SPACE njb-47 93 27 10 10 NUM njb-47 93 28     SPACE njb-47 93 29 12 12 NUM njb-47 93 30     SPACE njb-47 93 31 14 14 NUM njb-47 93 32     SPACE njb-47 93 33 y y PROPN njb-47 93 34 ie ie X njb-47 94 1 ld ld PROPN njb-47 94 2 o o X njb-47 95 1 f f PROPN njb-47 95 2 l l PROPN njb-47 95 3 ov ov PROPN njb-47 95 4 as as SCONJ njb-47 95 5 ta ta PROPN njb-47 95 6 ti ti PROPN njb-47 95 7 n n NOUN njb-47 95 8 ( ( PUNCT njb-47 95 9 m m NOUN njb-47 95 10 g/ g/ PROPN njb-47 95 11 g g X njb-47 95 12 ) ) PUNCT njb-47 95 13 carbon carbon NOUN njb-47 95 14 source source NOUN njb-47 95 15 nepal nepal ADJ njb-47 95 16     SPACE njb-47 95 17 journal journal NOUN njb-47 95 18     SPACE njb-47 95 19 of of ADP njb-47 95 20     SPACE njb-47 95 21 biotechnology biotechnology NOUN njb-47 95 22 . . PUNCT njb-47 95 23         SPACE njb-47 96 1 jan jan PROPN njb-47 96 2 . . PROPN njb-47 96 3     SPACE njb-47 96 4 2012 2012 NUM njb-47 96 5 , , PUNCT njb-47 96 6     SPACE njb-47 96 7 vol vol NOUN njb-47 96 8 . . PUNCT njb-47 96 9     SPACE njb-47 96 10 2 2 NUM njb-47 96 11 , , PUNCT njb-47 96 12     SPACE njb-47 96 13 no no INTJ njb-47 96 14 . . PUNCT njb-47 96 15     SPACE njb-47 97 1 1 1 NUM njb-47 97 2 : : PUNCT njb-47 97 3     SPACE njb-47 97 4 26 26 NUM njb-47 97 5     SPACE njb-47 97 6 – – PUNCT njb-47 97 7     SPACE njb-47 97 8 36 36 NUM njb-47 97 9                                                                                                                                                                                                                                             SPACE njb-47 97 10 biotechnology biotechnology NOUN njb-47 97 11     SPACE njb-47 97 12 society society NOUN njb-47 97 13     SPACE njb-47 97 14 of of ADP njb-47 97 15     SPACE njb-47 97 16 nepal nepal NOUN njb-47 97 17     SPACE njb-47 97 18 ( ( PUNCT njb-47 97 19 bsn bsn PROPN njb-47 97 20 ) ) PUNCT njb-47 97 21 , , PUNCT njb-47 97 22     SPACE njb-47 97 23 all all DET njb-47 97 24     SPACE njb-47 97 25 rights right NOUN njb-47 97 26     SPACE njb-47 97 27 reserved reserve VERB njb-47 97 28                 SPACE njb-47 97 29 34 34 NUM njb-47 97 30     SPACE njb-47 97 31 effect effect NOUN njb-47 97 32 of of ADP njb-47 97 33 nitrogen nitrogen PROPN njb-47 97 34 source source PROPN njb-47 97 35 nitrogen nitrogen PROPN njb-47 97 36 source source NOUN njb-47 97 37 can can AUX njb-47 97 38 be be AUX njb-47 97 39 an an DET njb-47 97 40 important important ADJ njb-47 97 41 limiting limit VERB njb-47 97 42 factor factor NOUN njb-47 97 43 in in ADP njb-47 97 44 the the DET njb-47 97 45 production production NOUN njb-47 97 46 of of ADP njb-47 97 47 microbial microbial ADJ njb-47 97 48 metabolites metabolite NOUN njb-47 97 49 [ [ X njb-47 97 50 14 14 NUM njb-47 97 51 ] ] PUNCT njb-47 97 52 . . PUNCT njb-47 98 1 the the DET njb-47 98 2 supplementation supplementation NOUN njb-47 98 3 of of ADP njb-47 98 4 additional additional ADJ njb-47 98 5 nitrogen nitrogen NOUN njb-47 98 6 sources source NOUN njb-47 98 7 ( ( PUNCT njb-47 98 8 either either CCONJ njb-47 98 9 organic organic ADJ njb-47 98 10 or or CCONJ njb-47 98 11 inorganic inorganic ADJ njb-47 98 12 ) ) PUNCT njb-47 98 13 such such ADJ njb-47 98 14 as as ADP njb-47 98 15 sodium sodium NOUN njb-47 98 16 nitrate nitrate NOUN njb-47 98 17 , , PUNCT njb-47 98 18 ammonium ammonium NOUN njb-47 98 19 sulphate sulphate NOUN njb-47 98 20 , , PUNCT njb-47 98 21 urea urea NOUN njb-47 98 22 , , PUNCT njb-47 98 23 peptone peptone NOUN njb-47 98 24 , , PUNCT njb-47 98 25 tryptone tryptone NOUN njb-47 98 26 , , PUNCT njb-47 98 27 yeast yeast NOUN njb-47 98 28 extract extract NOUN njb-47 98 29 , , PUNCT njb-47 98 30 malt malt NOUN njb-47 98 31 extract extract NOUN njb-47 98 32 and and CCONJ njb-47 98 33 beef beef NOUN njb-47 98 34 extract extract NOUN njb-47 98 35 at at ADP njb-47 98 36 1 1 NUM njb-47 98 37 % % NOUN njb-47 98 38 ( ( PUNCT njb-47 98 39 w w PROPN njb-47 98 40 / / SYM njb-47 98 41 v v NOUN njb-47 98 42 ) ) PUNCT njb-47 98 43 had have AUX njb-47 98 44 shown show VERB njb-47 98 45 a a DET njb-47 98 46 profound profound ADJ njb-47 98 47 impact impact NOUN njb-47 98 48 on on ADP njb-47 98 49 the the DET njb-47 98 50 production production NOUN njb-47 98 51 of of ADP njb-47 98 52 lovastatin lovastatin PROPN njb-47 98 53 by by ADP njb-47 98 54 a. a. PROPN njb-47 98 55 fischeri fischeri PROPN njb-47 98 56 under under ADP njb-47 98 57 ssf ssf PROPN njb-47 98 58 ( ( PUNCT njb-47 98 59 fig fig NOUN njb-47 98 60 . . PUNCT njb-47 98 61 7 7 NUM njb-47 98 62 ) ) PUNCT njb-47 98 63 . . PUNCT njb-47 99 1 among among ADP njb-47 99 2 the the DET njb-47 99 3 various various ADJ njb-47 99 4 nitrogen nitrogen NOUN njb-47 99 5 sources source NOUN njb-47 99 6 evaluated evaluate VERB njb-47 99 7 , , PUNCT njb-47 99 8 malt malt NOUN njb-47 99 9 extract extract NOUN njb-47 99 10 in in ADP njb-47 99 11 the the DET njb-47 99 12 fermentation fermentation NOUN njb-47 99 13 medium medium NOUN njb-47 99 14 promoted promote VERB njb-47 99 15 enhanced enhance VERB njb-47 99 16 fungal fungal ADJ njb-47 99 17 growth growth NOUN njb-47 99 18 and and CCONJ njb-47 99 19 consequent consequent ADJ njb-47 99 20 lovastatin lovastatin ADJ njb-47 99 21 production production NOUN njb-47 99 22 of of ADP njb-47 99 23 14.77 14.77 NUM njb-47 99 24 mg mg PROPN njb-47 99 25 / / SYM njb-47 99 26 g g NOUN njb-47 99 27 dry dry ADJ njb-47 99 28 substrate substrate NOUN njb-47 99 29 was be AUX njb-47 99 30 achieved achieve VERB njb-47 99 31 followed follow VERB njb-47 99 32 by by ADP njb-47 99 33 yeast yeast NOUN njb-47 99 34 extract extract NOUN njb-47 99 35 ( ( PUNCT njb-47 99 36 12.36 12.36 NUM njb-47 99 37 mg mg PROPN njb-47 99 38 / / SYM njb-47 99 39 g g NOUN njb-47 99 40 dry dry ADJ njb-47 99 41 substrate substrate NOUN njb-47 99 42 ) ) PUNCT njb-47 99 43 and and CCONJ njb-47 99 44 sodium sodium NOUN njb-47 99 45 nitrate nitrate NOUN njb-47 99 46 ( ( PUNCT njb-47 99 47 11.03 11.03 NUM njb-47 99 48 mg mg NUM njb-47 99 49 / / SYM njb-47 99 50 g g NOUN njb-47 99 51 dry dry ADJ njb-47 99 52 substrate substrate NOUN njb-47 99 53 ) ) PUNCT njb-47 99 54 . . PUNCT njb-47 100 1 fig fig NOUN njb-47 100 2 . . PUNCT njb-47 101 1 7 7 NUM njb-47 101 2 : : PUNCT njb-47 101 3 effect effect NOUN njb-47 101 4 of of ADP njb-47 101 5 different different ADJ njb-47 101 6 nitrogen nitrogen NOUN njb-47 101 7 sources source NOUN njb-47 101 8 on on ADP njb-47 101 9 lovastatin lovastatin ADJ njb-47 101 10 production production NOUN njb-47 101 11 conclusion conclusion NOUN njb-47 101 12 : : PUNCT njb-47 101 13 the the DET njb-47 101 14 current current ADJ njb-47 101 15 investigation investigation NOUN njb-47 101 16 was be AUX njb-47 101 17 mainly mainly ADV njb-47 101 18 focused focus VERB njb-47 101 19 on on ADP njb-47 101 20 the the DET njb-47 101 21 evaluation evaluation NOUN njb-47 101 22 of of ADP njb-47 101 23 the the DET njb-47 101 24 potentiality potentiality NOUN njb-47 101 25 of of ADP njb-47 101 26 aspergillus aspergillus PROPN njb-47 101 27 fischeri fischeri PROPN njb-47 101 28 for for ADP njb-47 101 29 utilization utilization NOUN njb-47 101 30 of of ADP njb-47 101 31 coconut coconut NOUN njb-47 101 32 oil oil NOUN njb-47 101 33 cake cake NOUN njb-47 101 34 as as ADP njb-47 101 35 the the DET njb-47 101 36 substrate substrate NOUN njb-47 101 37 for for ADP njb-47 101 38 the the DET njb-47 101 39 production production NOUN njb-47 101 40 of of ADP njb-47 101 41 lovastatin lovastatin PROPN njb-47 101 42 under under ADP njb-47 101 43 solid solid ADJ njb-47 101 44 state state NOUN njb-47 101 45 fermentation fermentation NOUN njb-47 101 46 . . PUNCT njb-47 102 1 the the DET njb-47 102 2 yields yield NOUN njb-47 102 3 obtained obtain VERB njb-47 102 4 in in ADP njb-47 102 5 the the DET njb-47 102 6 present present ADJ njb-47 102 7 study study NOUN njb-47 102 8 have have VERB njb-47 102 9 to to PART njb-47 102 10 be be AUX njb-47 102 11 further far ADV njb-47 102 12 increased increase VERB njb-47 102 13 for for ADP njb-47 102 14 its its PRON njb-47 102 15 industrial industrial ADJ njb-47 102 16 importance importance NOUN njb-47 102 17 . . PUNCT njb-47 103 1 it it PRON njb-47 103 2 has have AUX njb-47 103 3 proved prove VERB njb-47 103 4 the the DET njb-47 103 5 feasibility feasibility NOUN njb-47 103 6 of of ADP njb-47 103 7 solid solid ADJ njb-47 103 8 state state NOUN njb-47 103 9 fermentation fermentation NOUN njb-47 103 10 as as ADP njb-47 103 11 a a DET njb-47 103 12 promising promising ADJ njb-47 103 13 technique technique NOUN njb-47 103 14 in in ADP njb-47 103 15 exploiting exploit VERB njb-47 103 16 cheaply cheaply ADV njb-47 103 17 available available ADJ njb-47 103 18 agro agro ADJ njb-47 103 19 - - PUNCT njb-47 103 20 residual residual ADJ njb-47 103 21 wastes waste NOUN njb-47 103 22 as as ADP njb-47 103 23 substrates substrate NOUN njb-47 103 24 for for ADP njb-47 103 25 the the DET njb-47 103 26 large large ADJ njb-47 103 27 - - PUNCT njb-47 103 28 scale scale NOUN njb-47 103 29 production production NOUN njb-47 103 30 of of ADP njb-47 103 31 microbial microbial ADJ njb-47 103 32 metabolites metabolite NOUN njb-47 103 33 of of ADP njb-47 103 34 biotechnological biotechnological ADJ njb-47 103 35 importance importance NOUN njb-47 103 36 ultimately ultimately ADV njb-47 103 37 leading lead VERB njb-47 103 38 to to ADP njb-47 103 39 an an DET njb-47 103 40 effective effective ADJ njb-47 103 41 solid solid ADJ njb-47 103 42 waste waste NOUN njb-47 103 43 management management NOUN njb-47 103 44 . . PUNCT njb-47 104 1 0 0 NUM njb-47 104 2 2 2 NUM njb-47 104 3 4 4 NUM njb-47 104 4 6 6 NUM njb-47 104 5 8 8 NUM njb-47 104 6 10 10 NUM njb-47 104 7 12 12 NUM njb-47 104 8 14 14 NUM njb-47 104 9 16 16 NUM njb-47 104 10 y y PROPN njb-47 104 11 ie ie X njb-47 104 12 ld ld PROPN njb-47 105 1 o o X njb-47 106 1 f f PROPN njb-47 106 2 l l PROPN njb-47 106 3 ov ov PROPN njb-47 106 4 as as SCONJ njb-47 106 5 ta ta PROPN njb-47 106 6 ti ti PROPN njb-47 106 7 n n NOUN njb-47 106 8 ( ( PUNCT njb-47 106 9 m m NOUN njb-47 106 10 g/ g/ PROPN njb-47 106 11 g g X njb-47 106 12 ) ) PUNCT njb-47 106 13 nitrogen nitrogen PROPN njb-47 106 14 source source NOUN njb-47 106 15 nepal nepal ADJ njb-47 106 16     SPACE njb-47 106 17 journal journal NOUN njb-47 106 18     SPACE njb-47 106 19 of of ADP njb-47 106 20     SPACE njb-47 106 21 biotechnology biotechnology NOUN njb-47 106 22 . . PUNCT njb-47 106 23         SPACE njb-47 107 1 jan jan PROPN njb-47 107 2 . . PROPN njb-47 107 3     SPACE njb-47 107 4 2012 2012 NUM njb-47 107 5 , , PUNCT njb-47 107 6     SPACE njb-47 107 7 vol vol NOUN njb-47 107 8 . . PUNCT njb-47 107 9     SPACE njb-47 107 10 2 2 NUM njb-47 107 11 , , PUNCT njb-47 107 12     SPACE njb-47 107 13 no no INTJ njb-47 107 14 . . PUNCT njb-47 107 15     SPACE njb-47 108 1 1 1 NUM njb-47 108 2 : : PUNCT njb-47 108 3     SPACE njb-47 108 4 26 26 NUM njb-47 108 5     SPACE njb-47 108 6 – – PUNCT njb-47 108 7     SPACE njb-47 108 8 36 36 NUM njb-47 108 9                                                                                                                                                                                                                                             SPACE njb-47 108 10 biotechnology biotechnology NOUN njb-47 108 11     SPACE njb-47 108 12 society society NOUN njb-47 108 13     SPACE njb-47 108 14 of of ADP njb-47 108 15     SPACE njb-47 108 16 nepal nepal NOUN njb-47 108 17     SPACE njb-47 108 18 ( ( PUNCT njb-47 108 19 bsn bsn PROPN njb-47 108 20 ) ) PUNCT njb-47 108 21 , , PUNCT njb-47 108 22     SPACE njb-47 108 23 all all DET njb-47 108 24     SPACE njb-47 108 25 rights right NOUN njb-47 108 26     SPACE njb-47 108 27 reserved reserve VERB njb-47 108 28                 SPACE njb-47 108 29 35 35 NUM njb-47 108 30     SPACE njb-47 108 31 references reference NOUN njb-47 108 32 : : PUNCT njb-47 108 33 1 1 X njb-47 108 34 . . PUNCT njb-47 108 35 alberts alberts PROPN njb-47 108 36 aw aw INTJ njb-47 108 37 : : PUNCT njb-47 108 38 lovastatin lovastatin PROPN njb-47 108 39 and and CCONJ njb-47 108 40 simvastatin simvastatin ADJ njb-47 108 41 - - PUNCT njb-47 108 42 inhibitors inhibitor NOUN njb-47 108 43 of of ADP njb-47 108 44 hmg hmg PROPN njb-47 108 45 coa coa PROPN njb-47 108 46 reductase reductase NOUN njb-47 108 47 and and CCONJ njb-47 108 48 cholesterolbiosynthesis cholesterolbiosynthesis NOUN njb-47 108 49 : : PUNCT njb-47 108 50 cardiology cardiology NOUN njb-47 108 51 1990 1990 NUM njb-47 108 52 , , PUNCT njb-47 108 53 77:1421 77:1421 NUM njb-47 108 54 . . NOUN njb-47 108 55 2 2 NUM njb-47 108 56 . . PUNCT njb-47 108 57 alberts alberts PROPN njb-47 108 58 aw aw INTJ njb-47 108 59 , , PUNCT njb-47 108 60 chen chen PROPN njb-47 108 61 j j PROPN njb-47 108 62 , , PUNCT njb-47 108 63 kuron kuron PROPN njb-47 108 64 g g PROPN njb-47 108 65 , , PUNCT njb-47 108 66 hunt hunt PROPN njb-47 108 67 v v PROPN njb-47 108 68 , , PUNCT njb-47 108 69 huff huff PROPN njb-47 108 70 j j PROPN njb-47 108 71 , , PUNCT njb-47 108 72 hoffman hoffman PROPN njb-47 108 73 c c PROPN njb-47 108 74 , , PUNCT njb-47 108 75 rothrock rothrock PROPN njb-47 108 76 j j PROPN njb-47 108 77 , , PUNCT njb-47 108 78 lopez lopez PROPN njb-47 108 79 m m PROPN njb-47 108 80 , , PUNCT njb-47 108 81 joshu joshu PROPN njb-47 108 82 h h PROPN njb-47 108 83 , , PUNCT njb-47 108 84 harris harris PROPN njb-47 108 85 e e PROPN njb-47 108 86 , , PUNCT njb-47 108 87 patchett patchett PROPN njb-47 108 88 a a PRON njb-47 108 89 , , PUNCT njb-47 108 90 monaghans monaghans X njb-47 108 91 r r PROPN njb-47 108 92 , , PUNCT njb-47 108 93 currie currie PROPN njb-47 108 94 s s PROPN njb-47 108 95 , , PUNCT njb-47 108 96 stapley stapley PROPN njb-47 108 97 e e PROPN njb-47 108 98 , , PUNCT njb-47 108 99 alberts albert VERB njb-47 108 100 sg sg PROPN njb-47 108 101 , , PUNCT njb-47 108 102 hensens hensens PROPN njb-47 108 103 o o PROPN njb-47 108 104 , , PUNCT njb-47 108 105 hirshfield hirshfield PROPN njb-47 108 106 t t PROPN njb-47 108 107 , , PUNCT njb-47 108 108 hoogsteent hoogsteent PROPN njb-47 108 109 j j PROPN njb-47 108 110 , , PUNCT njb-47 108 111 liescht liescht PROPN njb-47 108 112 j j PROPN njb-47 108 113 , , PUNCT njb-47 108 114 springeri springeri PROPN njb-47 108 115 j j PROPN njb-47 108 116 : : PUNCT njb-47 108 117 mevinolin mevinolin PROPN njb-47 108 118 : : PUNCT njb-47 108 119 a a DET njb-47 108 120 highly highly ADV njb-47 108 121 potent potent ADJ njb-47 108 122 competitive competitive ADJ njb-47 108 123 inhibitor inhibitor NOUN njb-47 108 124 of of ADP njb-47 108 125 hydroxymethylglutaryl hydroxymethylglutaryl NOUN njb-47 108 126 - - PUNCT njb-47 108 127 coenzyme coenzyme NOUN njb-47 108 128 a a DET njb-47 108 129 reductase reductase NOUN njb-47 108 130 and and CCONJ njb-47 108 131 a a DET njb-47 108 132 cholesterol cholesterol NOUN njb-47 108 133 - - PUNCT njb-47 108 134 lowering lower VERB njb-47 108 135 agent agent NOUN njb-47 108 136 . . PUNCT njb-47 109 1 proc proc PROPN njb-47 109 2 . . PUNCT njb-47 110 1 natl natl PROPN njb-47 110 2 . . PUNCT njb-47 111 1 acad acad PROPN njb-47 111 2 . . PUNCT njb-47 112 1 sci sci PROPN njb-47 112 2 . . PUNCT njb-47 112 3 1980 1980 NUM njb-47 112 4 , , PUNCT njb-47 112 5 77 77 NUM njb-47 112 6 ( ( PUNCT njb-47 112 7 suppl suppl NOUN njb-47 112 8 . . PUNCT njb-47 113 1 7 7 NUM njb-47 113 2 ): ): PUNCT njb-47 113 3 3957 3957 NUM njb-47 113 4 - - SYM njb-47 113 5 3961 3961 NUM njb-47 113 6 . . PUNCT njb-47 114 1 3 3 X njb-47 114 2 . . X njb-47 114 3 seenivasan seenivasan VERB njb-47 114 4 a a DET njb-47 114 5 , , PUNCT njb-47 114 6 subhagar subhagar PROPN njb-47 114 7 s s PROPN njb-47 114 8 , , PUNCT njb-47 114 9 aravindan aravindan PROPN njb-47 114 10 r r PROPN njb-47 114 11 , , PUNCT njb-47 114 12 viruthagiri viruthagiri PROPN njb-47 114 13 t t PROPN njb-47 114 14 : : PUNCT njb-47 114 15 microbial microbial ADJ njb-47 114 16 production production NOUN njb-47 114 17 and and CCONJ njb-47 114 18 biomedical biomedical ADJ njb-47 114 19 applications application NOUN njb-47 114 20 of of ADP njb-47 114 21 lovastatin lovastatin PROPN njb-47 114 22 . . PUNCT njb-47 115 1 ind ind PROPN njb-47 115 2 . . PROPN njb-47 115 3 j. j. PROPN njb-47 115 4 of of ADP njb-47 115 5 pharm pharm PROPN njb-47 115 6 . . PUNCT njb-47 116 1 sciences science NOUN njb-47 116 2 2009 2009 NUM njb-47 116 3 , , PUNCT njb-47 116 4 701 701 NUM njb-47 116 5 - - SYM njb-47 116 6 709 709 NUM njb-47 116 7 . . NUM njb-47 116 8 4 4 NUM njb-47 116 9 . . PUNCT njb-47 116 10 jones jones PROPN njb-47 116 11 kd kd PROPN njb-47 116 12 , , PUNCT njb-47 116 13 couldwell couldwell PROPN njb-47 116 14 wt wt PROPN njb-47 116 15 , , PUNCT njb-47 116 16 hinton hinton PROPN njb-47 116 17 dr dr PROPN njb-47 116 18 , , PUNCT njb-47 116 19 su su PROPN njb-47 116 20 yh yh PROPN njb-47 116 21 , , PUNCT njb-47 116 22 he he PRON njb-47 116 23 dk dk INTJ njb-47 116 24 , , PUNCT njb-47 116 25 anker anker NOUN njb-47 116 26 l l PROPN njb-47 116 27 , , PUNCT njb-47 116 28 law law NOUN njb-47 116 29 re re NOUN njb-47 116 30 : : PUNCT njb-47 116 31 lovastatin lovastatin PROPN njb-47 116 32 induces induce VERB njb-47 116 33 growth growth NOUN njb-47 116 34 inhibition inhibition NOUN njb-47 116 35 and and CCONJ njb-47 116 36 apoptosis apoptosis NOUN njb-47 116 37 in in ADP njb-47 116 38 human human ADJ njb-47 116 39 malignant malignant ADJ njb-47 116 40 glioma glioma NOUN njb-47 116 41 cells cell NOUN njb-47 116 42 . . PUNCT njb-47 117 1 biochem biochem NOUN njb-47 117 2 . . PUNCT njb-47 118 1 biophys biophy NOUN njb-47 118 2 . . PUNCT njb-47 119 1 res res NOUN njb-47 119 2 .commun .commun PUNCT njb-47 119 3 . . PUNCT njb-47 119 4 1994 1994 NUM njb-47 119 5 , , PUNCT njb-47 119 6 205:1681 205:1681 NUM njb-47 119 7 - - SYM njb-47 119 8 1687 1687 NUM njb-47 119 9 . . PUNCT njb-47 120 1 5 5 X njb-47 120 2 . . X njb-47 120 3 newman newman PROPN njb-47 120 4 a a PROPN njb-47 120 5 , , PUNCT njb-47 120 6 clutterbuck clutterbuck PROPN njb-47 120 7 rd rd PROPN njb-47 120 8 , , PUNCT njb-47 120 9 powles powle VERB njb-47 120 10 rl rl PROPN njb-47 120 11 , , PUNCT njb-47 120 12 millar millar PROPN njb-47 120 13 jl jl PROPN njb-47 120 14 : : PUNCT njb-47 120 15 selective selective ADJ njb-47 120 16 inhibition inhibition NOUN njb-47 120 17 of of ADP njb-47 120 18 primary primary ADJ njb-47 120 19 acute acute PROPN njb-47 120 20 myeloid myeloid ADJ njb-47 120 21 leukemia leukemia NOUN njb-47 120 22 cell cell NOUN njb-47 120 23 growth growth NOUN njb-47 120 24 by by ADP njb-47 120 25 lovastatin lovastatin PROPN njb-47 120 26 . . PUNCT njb-47 121 1 leukemia leukemia NOUN njb-47 121 2 1994 1994 NUM njb-47 121 3 , , PUNCT njb-47 121 4 8:2022 8:2022 NUM njb-47 121 5 - - SYM njb-47 121 6 2029 2029 NUM njb-47 121 7 . . PUNCT njb-47 122 1 6 6 NUM njb-47 122 2 . . PUNCT njb-47 122 3 casas casas PROPN njb-47 122 4 lopez lopez PROPN njb-47 122 5 jl jl PROPN njb-47 122 6 , , PUNCT njb-47 122 7 sanchez sanchez PROPN njb-47 122 8 perez perez PROPN njb-47 122 9 ja ja PROPN njb-47 122 10 , , PUNCT njb-47 122 11 fernandez fernandez PROPN njb-47 122 12 sevilla sevilla PROPN njb-47 122 13 jm jm PROPN njb-47 122 14 , , PUNCT njb-47 122 15 acien acien PROPN njb-47 122 16 fernandez fernandez PROPN njb-47 122 17 fg fg PROPN njb-47 122 18 , , PUNCT njb-47 122 19 molina molina PROPN njb-47 122 20 grima grima PROPN njb-47 122 21 e e PROPN njb-47 122 22 , , PUNCT njb-47 122 23 chisti chisti PROPN njb-47 122 24 y y PROPN njb-47 122 25 : : PUNCT njb-47 122 26 production production NOUN njb-47 122 27 of of ADP njb-47 122 28 lovastatin lovastatin PROPN njb-47 122 29 by by ADP njb-47 122 30 aspergillus aspergillus NOUN njb-47 122 31 terreus terreus NOUN njb-47 122 32 : : PUNCT njb-47 122 33 effects effect NOUN njb-47 122 34 of of ADP njb-47 122 35 the the DET njb-47 122 36 c c NOUN njb-47 122 37 : : PUNCT njb-47 122 38 n n NOUN njb-47 122 39 ratio ratio NOUN njb-47 122 40 and and CCONJ njb-47 122 41 the the DET njb-47 122 42 principal principal ADJ njb-47 122 43 nutrients nutrient NOUN njb-47 122 44 on on ADP njb-47 122 45 growth growth NOUN njb-47 122 46 and and CCONJ njb-47 122 47 metabolite metabolite NOUN njb-47 122 48 production production NOUN njb-47 122 49 . . PUNCT njb-47 123 1 enzyme enzyme NOUN njb-47 123 2 microbiol microbiol ADJ njb-47 123 3 technol technol NOUN njb-47 123 4 . . PUNCT njb-47 123 5 2003 2003 NUM njb-47 123 6 , , PUNCT njb-47 123 7 33(suppl 33(suppl NUM njb-47 123 8 . . NOUN njb-47 123 9 2 2 NUM njb-47 123 10 - - SYM njb-47 123 11 3 3 NUM njb-47 123 12 ): ): PUNCT njb-47 123 13 270 270 NUM njb-47 123 14 - - SYM njb-47 123 15 277 277 NUM njb-47 123 16 . . NUM njb-47 124 1 7 7 NUM njb-47 124 2 . . PROPN njb-47 124 3 sitaram sitaram PROPN njb-47 124 4 kumar kumar PROPN njb-47 124 5 m m PROPN njb-47 124 6 , , PUNCT njb-47 124 7 jana jana PROPN njb-47 124 8 sk sk PROPN njb-47 124 9 , , PUNCT njb-47 124 10 senthil senthil PROPN njb-47 124 11 v v PROPN njb-47 124 12 , , PUNCT njb-47 124 13 shashanka shashanka PROPN njb-47 124 14 v v PROPN njb-47 124 15 , , PUNCT njb-47 124 16 vijayakumar vijayakumar PROPN njb-47 124 17 s s PROPN njb-47 124 18 , , PUNCT njb-47 124 19 sadhukhan sadhukhan PROPN njb-47 124 20 ak ak PROPN njb-47 124 21 : : PUNCT njb-47 124 22 repeated repeat VERB njb-47 124 23 fed fed PROPN njb-47 124 24 batch batch NOUN njb-47 124 25 process process NOUN njb-47 124 26 for for ADP njb-47 124 27 improving improve VERB njb-47 124 28 lovastatin lovastatin ADJ njb-47 124 29 production production NOUN njb-47 124 30 . . PUNCT njb-47 125 1 process process PROPN njb-47 125 2 biochem biochem PROPN njb-47 125 3 . . PUNCT njb-47 126 1 2000 2000 NUM njb-47 126 2 , , PUNCT njb-47 126 3 36 36 NUM njb-47 126 4 ( ( PUNCT njb-47 126 5 suppl suppl NOUN njb-47 126 6 . . PUNCT njb-47 127 1 4 4 NUM njb-47 127 2 ): ): PUNCT njb-47 127 3 363 363 NUM njb-47 127 4 - - SYM njb-47 127 5 368 368 NUM njb-47 127 6 . . NUM njb-47 127 7 8 8 NUM njb-47 127 8 . . X njb-47 127 9 valera valera PROPN njb-47 127 10 hr hr PROPN njb-47 127 11 , , PUNCT njb-47 127 12 gomes gomes PROPN njb-47 127 13 j j PROPN njb-47 127 14 , , PUNCT njb-47 127 15 lakshmi lakshmi PROPN njb-47 127 16 s s PROPN njb-47 127 17 , , PUNCT njb-47 127 18 gurujara gurujara PROPN njb-47 127 19 r r PROPN njb-47 127 20 , , PUNCT njb-47 127 21 suyanarayan suyanarayan PROPN njb-47 127 22 s s PROPN njb-47 127 23 , , PUNCT njb-47 127 24 kumar kumar PROPN njb-47 127 25 d d PROPN njb-47 127 26 : : PUNCT njb-47 127 27 lovastatin lovastatin ADJ njb-47 127 28 production production NOUN njb-47 127 29 by by ADP njb-47 127 30 solid solid ADJ njb-47 127 31 state state NOUN njb-47 127 32 fermentation fermentation NOUN njb-47 127 33 using use VERB njb-47 127 34 aspergillus aspergillus NOUN njb-47 127 35 flavipes flavipe NOUN njb-47 127 36 . . PUNCT njb-47 128 1 enzyme enzyme VERB njb-47 128 2 microbiol microbiol ADJ njb-47 128 3 technol technol NOUN njb-47 128 4 . . PUNCT njb-47 128 5 2005 2005 NUM njb-47 128 6 , , PUNCT njb-47 128 7 37 37 NUM njb-47 128 8 ( ( PUNCT njb-47 128 9 suppl suppl NOUN njb-47 128 10 . . PUNCT njb-47 129 1 5 5 NUM njb-47 129 2 ): ): PUNCT njb-47 129 3 521 521 NUM njb-47 129 4 - - SYM njb-47 129 5 526 526 NUM njb-47 129 6 . . PROPN njb-47 129 7 9 9 NUM njb-47 129 8 . . PUNCT njb-47 130 1 xu xu PROPN njb-47 130 2 bj bj PROPN njb-47 130 3 , , PUNCT njb-47 130 4 wang wang PROPN njb-47 130 5 qj qj PROPN njb-47 130 6 , , PUNCT njb-47 130 7 jia jia PROPN njb-47 130 8 xq xq PROPN njb-47 130 9 , , PUNCT njb-47 130 10 sung sing VERB njb-47 130 11 ck ck VERB njb-47 130 12 : : PUNCT njb-47 130 13 enhanced enhance VERB njb-47 130 14 lovastatin lovastatin ADJ njb-47 130 15 production production NOUN njb-47 130 16 by by ADP njb-47 130 17 solid solid ADJ njb-47 130 18 state state NOUN njb-47 130 19 fermentation fermentation NOUN njb-47 130 20 of of ADP njb-47 130 21 monascus monascus PROPN njb-47 130 22 ruber ruber PROPN njb-47 130 23 . . PUNCT njb-47 131 1 biotechnol biotechnol NOUN njb-47 131 2 and and CCONJ njb-47 131 3 bioprocess bioprocess NOUN njb-47 131 4 engg engg NOUN njb-47 131 5 . . PUNCT njb-47 131 6 2005 2005 NUM njb-47 131 7 , , PUNCT njb-47 131 8 10 10 NUM njb-47 131 9 ( ( PUNCT njb-47 131 10 suppl suppl NOUN njb-47 131 11 . . PUNCT njb-47 132 1 1):78 1):78 NUM njb-47 132 2 - - SYM njb-47 132 3 84 84 NUM njb-47 132 4 . . SYM njb-47 132 5 10 10 NUM njb-47 132 6 . . PUNCT njb-47 133 1 pie pie NOUN njb-47 133 2 - - PUNCT njb-47 133 3 lian lian ADJ njb-47 133 4 wei wei PROPN njb-47 133 5 , , PUNCT njb-47 133 6 zhi zhi PROPN njb-47 133 7 - - PUNCT njb-47 133 8 nan nan PROPN njb-47 133 9 xu xu PROPN njb-47 133 10 , , PUNCT njb-47 133 11 pei pei PROPN njb-47 133 12 - - PUNCT njb-47 133 13 lin lin PROPN njb-47 133 14 cen cen PROPN njb-47 133 15 : : PUNCT njb-47 133 16 lovastatin lovastatin ADJ njb-47 133 17 production production NOUN njb-47 133 18 by by ADP njb-47 133 19 aspergillus aspergillus NOUN njb-47 133 20 terreus terreus NOUN njb-47 133 21 in in ADP njb-47 133 22 solid solid ADJ njb-47 133 23 - - PUNCT njb-47 133 24 state state NOUN njb-47 133 25 fermentation fermentation NOUN njb-47 133 26 . . PUNCT njb-47 134 1 j. j. PROPN njb-47 134 2 zheijan zheijan PROPN njb-47 134 3 univ univ PROPN njb-47 134 4 . . PUNCT njb-47 135 1 2007 2007 NUM njb-47 135 2 , , PUNCT njb-47 135 3 9:1521 9:1521 NUM njb-47 135 4 - - SYM njb-47 135 5 1526 1526 NUM njb-47 135 6 . . PUNCT njb-47 136 1 11 11 NUM njb-47 136 2 . . PUNCT njb-47 137 1 mielcarek mielcarek PROPN njb-47 137 2 j j PROPN njb-47 137 3 , , PUNCT njb-47 137 4 naskreni naskreni PROPN njb-47 137 5 m m PROPN njb-47 137 6 , , PUNCT njb-47 137 7 grobelny grobelny PROPN njb-47 137 8 p p PROPN njb-47 137 9 : : PUNCT njb-47 137 10 photochemical photochemical ADJ njb-47 137 11 properties property NOUN njb-47 137 12 of of ADP njb-47 137 13 simvastatin simvastatin PROPN njb-47 137 14 and and CCONJ njb-47 137 15 lovastatin lovastatin ADJ njb-47 137 16 by by ADP njb-47 137 17 radiation radiation NOUN njb-47 137 18 . . PUNCT njb-47 138 1 j. j. PROPN njb-47 138 2 thermal thermal PROPN njb-47 138 3 analysis analysis PROPN njb-47 138 4 calorimetry calorimetry NOUN njb-47 138 5 2009 2009 NUM njb-47 138 6 , , PUNCT njb-47 138 7 96:301 96:301 NUM njb-47 138 8 - - SYM njb-47 138 9 305 305 NUM njb-47 138 10 . . NOUN njb-47 138 11 12 12 NUM njb-47 138 12 . . PUNCT njb-47 139 1 pansuriya pansuriya PROPN njb-47 139 2 rc rc PROPN njb-47 139 3 , , PUNCT njb-47 139 4 singhal singhal PROPN njb-47 139 5 rs rs PROPN njb-47 139 6 : : PUNCT njb-47 139 7 response response NOUN njb-47 139 8 surface surface NOUN njb-47 139 9 methodology methodology NOUN njb-47 139 10 for for ADP njb-47 139 11 optimization optimization NOUN njb-47 139 12 of of ADP njb-47 139 13 production production NOUN njb-47 139 14 of of ADP njb-47 139 15 lovastatin lovastatin PROPN njb-47 139 16 by by ADP njb-47 139 17 solid solid ADJ njb-47 139 18 state state NOUN njb-47 139 19 fermentation fermentation NOUN njb-47 139 20 . . PUNCT njb-47 140 1 brazilian brazilian PROPN njb-47 140 2 j. j. PROPN njb-47 140 3 microbiol microbiol PROPN njb-47 140 4 . . PUNCT njb-47 141 1 2010 2010 NUM njb-47 141 2 , , PUNCT njb-47 141 3 41:164 41:164 NUM njb-47 141 4 - - SYM njb-47 141 5 172 172 NUM njb-47 141 6 . . PUNCT njb-47 141 7 13 13 NUM njb-47 141 8 . . PUNCT njb-47 142 1 panda panda PROPN njb-47 142 2 bp bp PROPN njb-47 142 3 , , PUNCT njb-47 142 4 javed javed PROPN njb-47 142 5 s s PROPN njb-47 142 6 , , PUNCT njb-47 142 7 ali ali PROPN njb-47 142 8 m m PROPN njb-47 142 9 : : PUNCT njb-47 142 10 optimization optimization NOUN njb-47 142 11 of of ADP njb-47 142 12 fermentation fermentation NOUN njb-47 142 13 parameters parameter NOUN njb-47 142 14 for for ADP njb-47 142 15 higher high ADJ njb-47 142 16 lovastatin lovastatin ADJ njb-47 142 17 nepal nepal ADJ njb-47 142 18     SPACE njb-47 142 19 journal journal NOUN njb-47 142 20     SPACE njb-47 142 21 of of ADP njb-47 142 22     SPACE njb-47 142 23 biotechnology biotechnology NOUN njb-47 142 24 . . PUNCT njb-47 142 25         SPACE njb-47 143 1 jan jan PROPN njb-47 143 2 . . PROPN njb-47 143 3     SPACE njb-47 143 4 2012 2012 NUM njb-47 143 5 , , PUNCT njb-47 143 6     SPACE njb-47 143 7 vol vol NOUN njb-47 143 8 . . PUNCT njb-47 143 9     SPACE njb-47 143 10 2 2 NUM njb-47 143 11 , , PUNCT njb-47 143 12     SPACE njb-47 143 13 no no INTJ njb-47 143 14 . . PUNCT njb-47 143 15     SPACE njb-47 144 1 1 1 NUM njb-47 144 2 : : PUNCT njb-47 144 3     SPACE njb-47 144 4 26 26 NUM njb-47 144 5     SPACE njb-47 144 6 – – PUNCT njb-47 144 7     SPACE njb-47 144 8 36 36 NUM njb-47 144 9                                                                                                                                                                                                                                             SPACE njb-47 144 10 biotechnology biotechnology NOUN njb-47 144 11     SPACE njb-47 144 12 society society NOUN njb-47 144 13     SPACE njb-47 144 14 of of ADP njb-47 144 15     SPACE njb-47 144 16 nepal nepal NOUN njb-47 144 17     SPACE njb-47 144 18 ( ( PUNCT njb-47 144 19 bsn bsn PROPN njb-47 144 20 ) ) PUNCT njb-47 144 21 , , PUNCT njb-47 144 22     SPACE njb-47 144 23 all all DET njb-47 144 24     SPACE njb-47 144 25 rights right NOUN njb-47 144 26     SPACE njb-47 144 27 reserved reserve VERB njb-47 144 28                 SPACE njb-47 144 29 36 36 NUM njb-47 144 30     SPACE njb-47 144 31 production production NOUN njb-47 144 32 in in ADP njb-47 144 33 red red ADJ njb-47 144 34 mold mold NOUN njb-47 144 35 rice rice NOUN njb-47 144 36 through through ADP njb-47 144 37 co co NOUN njb-47 144 38 - - NOUN njb-47 144 39 culture culture NOUN njb-47 144 40 of of ADP njb-47 144 41 monascus monascus PROPN njb-47 144 42 purpureus purpureus PROPN njb-47 144 43 and and CCONJ njb-47 144 44 monascus monascus ADJ njb-47 144 45 ruber ruber PROPN njb-47 144 46 . . PUNCT njb-47 145 1 food food NOUN njb-47 145 2 bioprocess bioprocess NOUN njb-47 145 3 technol technol NOUN njb-47 145 4 . . PUNCT njb-47 145 5 2008 2008 NUM njb-47 145 6 , , PUNCT njb-47 145 7 53:342 53:342 NUM njb-47 145 8 - - SYM njb-47 145 9 346 346 NUM njb-47 145 10 . . PROPN njb-47 145 11 14 14 NUM njb-47 145 12 . . PUNCT njb-47 146 1 chandrasekaran chandrasekaran PROPN njb-47 146 2 m m PROPN njb-47 146 3 , , PUNCT njb-47 146 4 lashmanaperumalsamy lashmanaperumalsamy PROPN njb-47 146 5 p p PROPN njb-47 146 6 , , PUNCT njb-47 146 7 chandramohan chandramohan PROPN njb-47 146 8 d d PROPN njb-47 146 9 : : PUNCT njb-47 146 10 combined combine VERB njb-47 146 11 effect effect NOUN njb-47 146 12 of of ADP njb-47 146 13 environmental environmental ADJ njb-47 146 14 factors factor NOUN njb-47 146 15 on on ADP njb-47 146 16 spoilage spoilage ADJ njb-47 146 17 bacteria bacteria NOUN njb-47 146 18 . . PUNCT njb-47 147 1 fish fish NOUN njb-47 147 2 technology technology PROPN njb-47 147 3 ( ( PUNCT njb-47 147 4 india india PROPN njb-47 147 5 ) ) PUNCT njb-47 147 6 1991 1991 NUM njb-47 147 7 , , PUNCT njb-47 147 8 28 28 NUM njb-47 147 9 : : SYM njb-47 147 10 146 146 NUM njb-47 147 11 - - SYM njb-47 147 12 153 153 NUM njb-47 147 13 . . PUNCT njb-47 147 14                         SPACE