id sid tid token lemma pos njb-55 1 1 4 4 NUM njb-55 1 2 nepal nepal ADJ njb-55 1 3     SPACE njb-55 1 4 journal journal NOUN njb-55 1 5     SPACE njb-55 1 6 of of ADP njb-55 1 7     SPACE njb-55 1 8 biotechnology biotechnology NOUN njb-55 1 9 . . PUNCT njb-55 1 10     SPACE njb-55 2 1 jan jan PROPN njb-55 2 2 . . PROPN njb-55 2 3     SPACE njb-55 2 4 2011 2011 NUM njb-55 2 5 , , PUNCT njb-55 2 6     SPACE njb-55 2 7 vol vol NOUN njb-55 2 8 . . PUNCT njb-55 2 9     SPACE njb-55 2 10 1 1 NUM njb-55 2 11 , , PUNCT njb-55 2 12     SPACE njb-55 2 13 no no INTJ njb-55 2 14 . . PUNCT njb-55 2 15     SPACE njb-55 3 1 1 1 NUM njb-55 3 2 : : PUNCT njb-55 3 3     SPACE njb-55 3 4 22‐30 22‐30 NUM njb-55 3 5     SPACE njb-55 3 6 22 22 NUM njb-55 3 7     SPACE njb-55 3 8 biotechnology biotechnology NOUN njb-55 3 9     SPACE njb-55 3 10 society society NOUN njb-55 3 11     SPACE njb-55 3 12 of of ADP njb-55 3 13     SPACE njb-55 3 14 nepal nepal NOUN njb-55 3 15     SPACE njb-55 3 16 ( ( PUNCT njb-55 3 17 bsn bsn PROPN njb-55 3 18 ) ) PUNCT njb-55 3 19 , , PUNCT njb-55 3 20     SPACE njb-55 3 21 all all DET njb-55 3 22     SPACE njb-55 3 23 rights right NOUN njb-55 3 24     SPACE njb-55 3 25 reserved reserve VERB njb-55 3 26       SPACE njb-55 3 27 original original ADJ njb-55 3 28     SPACE njb-55 3 29 research research NOUN njb-55 3 30     SPACE njb-55 3 31 article article NOUN njb-55 3 32     SPACE njb-55 3 33 development development NOUN njb-55 3 34     SPACE njb-55 3 35 of of ADP njb-55 3 36     SPACE njb-55 3 37 pcr pcr NOUN njb-55 3 38     SPACE njb-55 3 39 assay assay NOUN njb-55 3 40     SPACE njb-55 3 41 for for ADP njb-55 3 42     SPACE njb-55 3 43 targeting target VERB njb-55 3 44     SPACE njb-55 3 45 partial partial ADJ njb-55 3 46     SPACE njb-55 3 47 lipl21 lipl21 NOUN njb-55 3 48     SPACE njb-55 3 49 and and CCONJ njb-55 3 50     SPACE njb-55 3 51 lipl41 lipl41 PROPN njb-55 3 52     SPACE njb-55 3 53 gene gene NOUN njb-55 3 54     SPACE njb-55 3 55 of of ADP njb-55 3 56     SPACE njb-55 3 57 leptospira leptospira PROPN njb-55 3 58     SPACE njb-55 3 59 s. s. PROPN njb-55 3 60     SPACE njb-55 3 61 chandan1 chandan1 PROPN njb-55 3 62 , , PUNCT njb-55 3 63     SPACE njb-55 3 64 s. s. PROPN njb-55 3 65     SPACE njb-55 3 66 umesha1 umesha1 PROPN njb-55 3 67 , , PUNCT njb-55 3 68     SPACE njb-55 3 69 s.k s.k PROPN njb-55 3 70 . . PROPN njb-55 3 71     SPACE njb-55 3 72 bhure2 bhure2 PROPN njb-55 3 73 , , PUNCT njb-55 3 74     SPACE njb-55 3 75 n. n. PROPN njb-55 3 76     SPACE njb-55 3 77 haraprasad3 haraprasad3 PROPN njb-55 3 78 , , PUNCT njb-55 3 79     SPACE njb-55 3 80 s. s. PROPN njb-55 3 81     SPACE njb-55 3 82 chandrashekar1 chandrashekar1 PROPN njb-55 3 83     SPACE njb-55 3 84 1 1 NUM njb-55 3 85     SPACE njb-55 3 86 department department NOUN njb-55 3 87     SPACE njb-55 3 88 of of ADP njb-55 3 89     SPACE njb-55 3 90 studies study NOUN njb-55 3 91     SPACE njb-55 3 92 in in ADP njb-55 3 93     SPACE njb-55 3 94 biotechnology biotechnology NOUN njb-55 3 95 , , PUNCT njb-55 3 96     SPACE njb-55 3 97 university university NOUN njb-55 3 98     SPACE njb-55 3 99 of of ADP njb-55 3 100     SPACE njb-55 3 101 mysore mysore NOUN njb-55 3 102 , , PUNCT njb-55 3 103     SPACE njb-55 3 104 manasagangotri manasagangotri ADJ njb-55 3 105 , , PUNCT njb-55 3 106     SPACE njb-55 3 107 mysore‐570006 mysore‐570006 NOUN njb-55 3 108 , , PUNCT njb-55 3 109     SPACE njb-55 3 110 karnataka karnataka PROPN njb-55 3 111 , , PUNCT njb-55 3 112     SPACE njb-55 3 113 india india PROPN njb-55 3 114 . . PUNCT njb-55 3 115     SPACE njb-55 4 1 2 2 NUM njb-55 4 2     SPACE njb-55 4 3 ivri ivri PROPN njb-55 4 4 , , PUNCT njb-55 4 5     SPACE njb-55 4 6 izatnagar izatnagar ADJ njb-55 4 7 , , PUNCT njb-55 4 8     SPACE njb-55 4 9 bareilly‐243 bareilly‐243 PROPN njb-55 4 10     SPACE njb-55 4 11 122 122 NUM njb-55 4 12 , , PUNCT njb-55 4 13     SPACE njb-55 4 14 uttar uttar ADJ njb-55 4 15     SPACE njb-55 4 16 pradesh pradesh NOUN njb-55 4 17 , , PUNCT njb-55 4 18     SPACE njb-55 4 19 india india PROPN njb-55 4 20 . . PUNCT njb-55 4 21     SPACE njb-55 5 1 3 3 NUM njb-55 5 2     SPACE njb-55 5 3 department department NOUN njb-55 5 4     SPACE njb-55 5 5 of of ADP njb-55 5 6     SPACE njb-55 5 7 biotechnology biotechnology NOUN njb-55 5 8 , , PUNCT njb-55 5 9     SPACE njb-55 5 10 sj sj PROPN njb-55 5 11     SPACE njb-55 5 12 college college NOUN njb-55 5 13     SPACE njb-55 5 14 of of ADP njb-55 5 15     SPACE njb-55 5 16 engineering engineering NOUN njb-55 5 17 , , PUNCT njb-55 5 18     SPACE njb-55 5 19 manasagangotri manasagangotri ADJ njb-55 5 20 , , PUNCT njb-55 5 21     SPACE njb-55 5 22 mysore‐570006 mysore‐570006 NOUN njb-55 5 23 , , PUNCT njb-55 5 24     SPACE njb-55 5 25 karnataka karnataka PROPN njb-55 5 26 , , PUNCT njb-55 5 27     SPACE njb-55 5 28 india india PROPN njb-55 5 29 . . PUNCT njb-55 5 30     SPACE njb-55 6 1 abstract abstract ADJ njb-55 6 2     SPACE njb-55 6 3 leptospirosis leptospirosis NOUN njb-55 6 4     SPACE njb-55 6 5 is be AUX njb-55 6 6     SPACE njb-55 6 7 a a DET njb-55 6 8     SPACE njb-55 6 9 bacterial bacterial ADJ njb-55 6 10     SPACE njb-55 6 11 zoonotic zoonotic ADJ njb-55 6 12     SPACE njb-55 6 13 disease disease NOUN njb-55 6 14     SPACE njb-55 6 15 caused cause VERB njb-55 6 16     SPACE njb-55 6 17 by by ADP njb-55 6 18     SPACE njb-55 6 19 spirochetes spirochete NOUN njb-55 6 20     SPACE njb-55 6 21 of of ADP njb-55 6 22     SPACE njb-55 6 23 the the DET njb-55 6 24     SPACE njb-55 6 25 genus genus NOUN njb-55 6 26     SPACE njb-55 6 27 leptospira leptospira PROPN njb-55 6 28     SPACE njb-55 6 29 that that PRON njb-55 6 30     SPACE njb-55 6 31 affects affect VERB njb-55 6 32     SPACE njb-55 6 33 human human NOUN njb-55 6 34     SPACE njb-55 6 35 and and CCONJ njb-55 6 36     SPACE njb-55 6 37 a a DET njb-55 6 38     SPACE njb-55 6 39 wide wide ADJ njb-55 6 40     SPACE njb-55 6 41 range range NOUN njb-55 6 42     SPACE njb-55 6 43 of of ADP njb-55 6 44     SPACE njb-55 6 45 animals animal NOUN njb-55 6 46 . . PUNCT njb-55 6 47     SPACE njb-55 7 1 the the DET njb-55 7 2     SPACE njb-55 7 3 direct direct ADJ njb-55 7 4     SPACE njb-55 7 5 method method NOUN njb-55 7 6     SPACE njb-55 7 7 of of ADP njb-55 7 8     SPACE njb-55 7 9 diagnosis diagnosis NOUN njb-55 7 10     SPACE njb-55 7 11 of of ADP njb-55 7 12     SPACE njb-55 7 13 leptospirosis leptospirosis NOUN njb-55 7 14 , , PUNCT njb-55 7 15     SPACE njb-55 7 16 has have AUX njb-55 7 17     SPACE njb-55 7 18 been be AUX njb-55 7 19     SPACE njb-55 7 20 so so ADV njb-55 7 21     SPACE njb-55 7 22 far far ADV njb-55 7 23     SPACE njb-55 7 24 by by ADP njb-55 7 25     SPACE njb-55 7 26 culture culture NOUN njb-55 7 27     SPACE njb-55 7 28 isolation isolation NOUN njb-55 7 29     SPACE njb-55 7 30 but but CCONJ njb-55 7 31     SPACE njb-55 7 32 it it PRON njb-55 7 33     SPACE njb-55 7 34 is be AUX njb-55 7 35     SPACE njb-55 7 36 time time NOUN njb-55 7 37     SPACE njb-55 7 38 consuming consuming NOUN njb-55 7 39     SPACE njb-55 7 40 and and CCONJ njb-55 7 41     SPACE njb-55 7 42 potentially potentially ADV njb-55 7 43     SPACE njb-55 7 44 biohazardous biohazardous ADJ njb-55 7 45 . . PUNCT njb-55 7 46     SPACE njb-55 7 47 another another DET njb-55 7 48     SPACE njb-55 7 49 traditional traditional ADJ njb-55 7 50     SPACE njb-55 7 51 method method NOUN njb-55 7 52     SPACE njb-55 7 53 is be AUX njb-55 7 54     SPACE njb-55 7 55 the the DET njb-55 7 56     SPACE njb-55 7 57 detection detection NOUN njb-55 7 58     SPACE njb-55 7 59 of of ADP njb-55 7 60     SPACE njb-55 7 61 antibodies antibody NOUN njb-55 7 62     SPACE njb-55 7 63 ( ( PUNCT njb-55 7 64 serological serological ADJ njb-55 7 65     SPACE njb-55 7 66 tests test NOUN njb-55 7 67 ) ) PUNCT njb-55 7 68     SPACE njb-55 7 69 which which PRON njb-55 7 70     SPACE njb-55 7 71 is be AUX njb-55 7 72     SPACE njb-55 7 73 also also ADV njb-55 7 74     SPACE njb-55 7 75 a a DET njb-55 7 76     SPACE njb-55 7 77 time time NOUN njb-55 7 78     SPACE njb-55 7 79 consuming consuming NOUN njb-55 7 80     SPACE njb-55 7 81 method method NOUN njb-55 7 82     SPACE njb-55 7 83 and and CCONJ njb-55 7 84     SPACE njb-55 7 85 fails fail VERB njb-55 7 86     SPACE njb-55 7 87 to to PART njb-55 7 88     SPACE njb-55 7 89 identify identify VERB njb-55 7 90     SPACE njb-55 7 91 the the DET njb-55 7 92     SPACE njb-55 7 93 infecting infect VERB njb-55 7 94     SPACE njb-55 7 95 serovar serovar NOUN njb-55 7 96 . . PUNCT njb-55 7 97     SPACE njb-55 7 98 to to PART njb-55 7 99     SPACE njb-55 7 100 overcome overcome VERB njb-55 7 101     SPACE njb-55 7 102 these these DET njb-55 7 103     SPACE njb-55 7 104 limitations limitation NOUN njb-55 7 105     SPACE njb-55 7 106 associated associate VERB njb-55 7 107     SPACE njb-55 7 108 with with ADP njb-55 7 109     SPACE njb-55 7 110 the the DET njb-55 7 111     SPACE njb-55 7 112 cultivation cultivation NOUN njb-55 7 113     SPACE njb-55 7 114 and and CCONJ njb-55 7 115     SPACE njb-55 7 116 serology serology NOUN njb-55 7 117 , , PUNCT njb-55 7 118     SPACE njb-55 7 119 we we PRON njb-55 7 120     SPACE njb-55 7 121 developed develop VERB njb-55 7 122     SPACE njb-55 7 123 pcr pcr PROPN njb-55 7 124     SPACE njb-55 7 125 assay assay NOUN njb-55 7 126     SPACE njb-55 7 127 targeting target VERB njb-55 7 128     SPACE njb-55 7 129 partial partial ADJ njb-55 7 130     SPACE njb-55 7 131 lipl21 lipl21 NOUN njb-55 7 132     SPACE njb-55 7 133 gene gene NOUN njb-55 7 134     SPACE njb-55 7 135 and and CCONJ njb-55 7 136     SPACE njb-55 7 137 lipl41 lipl41 PROPN njb-55 7 138     SPACE njb-55 7 139 gene gene NOUN njb-55 7 140     SPACE njb-55 7 141 of of ADP njb-55 7 142     SPACE njb-55 7 143 leptospires leptospire NOUN njb-55 7 144     SPACE njb-55 7 145 using use VERB njb-55 7 146     SPACE njb-55 7 147 in‐house in‐house PROPN njb-55 7 148     SPACE njb-55 7 149 designed design VERB njb-55 7 150     SPACE njb-55 7 151 p28/29 p28/29 NOUN njb-55 7 152     SPACE njb-55 7 153 and and CCONJ njb-55 7 154     SPACE njb-55 7 155 p30/31 p30/31 ADJ njb-55 7 156     SPACE njb-55 7 157 primers primer NOUN njb-55 7 158 , , PUNCT njb-55 7 159     SPACE njb-55 7 160 with with ADP njb-55 7 161     SPACE njb-55 7 162 a a DET njb-55 7 163     SPACE njb-55 7 164 product product NOUN njb-55 7 165     SPACE njb-55 7 166 size size NOUN njb-55 7 167     SPACE njb-55 7 168 of of ADP njb-55 7 169     SPACE njb-55 7 170 385bp 385bp NOUN njb-55 7 171     SPACE njb-55 7 172 and and CCONJ njb-55 7 173     SPACE njb-55 7 174 427bp 427bp PROPN njb-55 7 175 . . PUNCT njb-55 7 176     SPACE njb-55 8 1 the the DET njb-55 8 2     SPACE njb-55 8 3 amplicons amplicon NOUN njb-55 8 4     SPACE njb-55 8 5 were be AUX njb-55 8 6     SPACE njb-55 8 7 subjected subject VERB njb-55 8 8     SPACE njb-55 8 9 to to PART njb-55 8 10     SPACE njb-55 8 11 restriction restriction NOUN njb-55 8 12     SPACE njb-55 8 13 enzyme enzyme NOUN njb-55 8 14     SPACE njb-55 8 15 digestion digestion NOUN njb-55 8 16     SPACE njb-55 8 17 using use VERB njb-55 8 18     SPACE njb-55 8 19 rsai rsai NOUN njb-55 8 20 , , PUNCT njb-55 8 21     SPACE njb-55 8 22 pvu pvu PROPN njb-55 8 23     SPACE njb-55 8 24 ii ii PROPN njb-55 8 25     SPACE njb-55 8 26 and and CCONJ njb-55 8 27     SPACE njb-55 8 28 hindiii hindiii PROPN njb-55 8 29     SPACE njb-55 8 30 for for ADP njb-55 8 31     SPACE njb-55 8 32 product product NOUN njb-55 8 33     SPACE njb-55 8 34 of of ADP njb-55 8 35     SPACE njb-55 8 36 p28/29 p28/29 NOUN njb-55 8 37     SPACE njb-55 8 38 and and CCONJ njb-55 8 39     SPACE njb-55 8 40 clai clai PROPN njb-55 8 41 , , PUNCT njb-55 8 42     SPACE njb-55 8 43 taqi taqi ADJ njb-55 8 44     SPACE njb-55 8 45 and and CCONJ njb-55 8 46     SPACE njb-55 8 47 rsai rsai PROPN njb-55 8 48     SPACE njb-55 8 49 were be AUX njb-55 8 50     SPACE njb-55 8 51 used use VERB njb-55 8 52     SPACE njb-55 8 53 for for ADP njb-55 8 54     SPACE njb-55 8 55 product product NOUN njb-55 8 56     SPACE njb-55 8 57 of of ADP njb-55 8 58     SPACE njb-55 8 59 p30/31 p30/31 NUM njb-55 8 60 . . PUNCT njb-55 8 61     SPACE njb-55 9 1 the the DET njb-55 9 2     SPACE njb-55 9 3 protocols protocol NOUN njb-55 9 4     SPACE njb-55 9 5 were be AUX njb-55 9 6     SPACE njb-55 9 7 standardized standardize VERB njb-55 9 8     SPACE njb-55 9 9 and and CCONJ njb-55 9 10     SPACE njb-55 9 11 the the DET njb-55 9 12     SPACE njb-55 9 13 assay assay NOUN njb-55 9 14     SPACE njb-55 9 15 targeting target VERB njb-55 9 16     SPACE njb-55 9 17 the the DET njb-55 9 18     SPACE njb-55 9 19 partial partial ADJ njb-55 9 20     SPACE njb-55 9 21 lipl21 lipl21 NOUN njb-55 9 22     SPACE njb-55 9 23 and and CCONJ njb-55 9 24     SPACE njb-55 9 25 lipl41 lipl41 PROPN njb-55 9 26     SPACE njb-55 9 27 gene gene NOUN njb-55 9 28     SPACE njb-55 9 29 was be AUX njb-55 9 30     SPACE njb-55 9 31 found find VERB njb-55 9 32     SPACE njb-55 9 33 to to PART njb-55 9 34     SPACE njb-55 9 35 be be AUX njb-55 9 36     SPACE njb-55 9 37 specific specific ADJ njb-55 9 38     SPACE njb-55 9 39 for for ADP njb-55 9 40     SPACE njb-55 9 41 eight eight NUM njb-55 9 42     SPACE njb-55 9 43 pathogenic pathogenic ADJ njb-55 9 44     SPACE njb-55 9 45 leptospires leptospire NOUN njb-55 9 46     SPACE njb-55 9 47 out out ADP njb-55 9 48     SPACE njb-55 9 49 of of ADP njb-55 9 50     SPACE njb-55 9 51 nine nine NUM njb-55 9 52     SPACE njb-55 9 53 leptospires leptospire NOUN njb-55 9 54     SPACE njb-55 9 55 tested test VERB njb-55 9 56 . . PUNCT njb-55 9 57     SPACE njb-55 10 1 the the DET njb-55 10 2     SPACE njb-55 10 3 products product NOUN njb-55 10 4     SPACE njb-55 10 5 were be AUX njb-55 10 6     SPACE njb-55 10 7 then then ADV njb-55 10 8     SPACE njb-55 10 9 cloned clone VERB njb-55 10 10     SPACE njb-55 10 11 in in ADP njb-55 10 12     SPACE njb-55 10 13 pgemt pgemt NOUN njb-55 10 14     SPACE njb-55 10 15 easy easy ADJ njb-55 10 16     SPACE njb-55 10 17 vector vector NOUN njb-55 10 18     SPACE njb-55 10 19 and and CCONJ njb-55 10 20     SPACE njb-55 10 21 sequenced sequence VERB njb-55 10 22     SPACE njb-55 10 23 to to PART njb-55 10 24     SPACE njb-55 10 25 facilitate facilitate VERB njb-55 10 26     SPACE njb-55 10 27 further further ADJ njb-55 10 28     SPACE njb-55 10 29 studies study NOUN njb-55 10 30 . . PUNCT njb-55 10 31       SPACE njb-55 11 1 pcr pcr PROPN njb-55 11 2     SPACE njb-55 11 3 could could AUX njb-55 11 4     SPACE njb-55 11 5 detect detect VERB njb-55 11 6     SPACE njb-55 11 7 the the DET njb-55 11 8     SPACE njb-55 11 9 target target NOUN njb-55 11 10     SPACE njb-55 11 11 bacterial bacterial ADJ njb-55 11 12     SPACE njb-55 11 13 gene gene NOUN njb-55 11 14     SPACE njb-55 11 15 without without ADP njb-55 11 16     SPACE njb-55 11 17 any any DET njb-55 11 18     SPACE njb-55 11 19 ambiguity ambiguity NOUN njb-55 11 20     SPACE njb-55 11 21 and and CCONJ njb-55 11 22     SPACE njb-55 11 23 showed show VERB njb-55 11 24     SPACE njb-55 11 25 good good ADJ njb-55 11 26     SPACE njb-55 11 27 efficiency efficiency NOUN njb-55 11 28     SPACE njb-55 11 29 in in ADP njb-55 11 30     SPACE njb-55 11 31 detection detection NOUN njb-55 11 32     SPACE njb-55 11 33 of of ADP njb-55 11 34     SPACE njb-55 11 35 targeted target VERB njb-55 11 36     SPACE njb-55 11 37 species specie NOUN njb-55 11 38     SPACE njb-55 11 39 in in ADP njb-55 11 40     SPACE njb-55 11 41 the the DET njb-55 11 42     SPACE njb-55 11 43 sample sample NOUN njb-55 11 44 . . PUNCT njb-55 11 45     SPACE njb-55 12 1 this this DET njb-55 12 2     SPACE njb-55 12 3 simple simple ADJ njb-55 12 4 , , PUNCT njb-55 12 5     SPACE njb-55 12 6 rapid rapid ADJ njb-55 12 7     SPACE njb-55 12 8 and and CCONJ njb-55 12 9     SPACE njb-55 12 10 cost‐effective cost‐effective NOUN njb-55 12 11     SPACE njb-55 12 12 method method NOUN njb-55 12 13     SPACE njb-55 12 14 can can AUX njb-55 12 15     SPACE njb-55 12 16 be be AUX njb-55 12 17     SPACE njb-55 12 18 applicable applicable ADJ njb-55 12 19     SPACE njb-55 12 20 in in ADP njb-55 12 21     SPACE njb-55 12 22 a a DET njb-55 12 23     SPACE njb-55 12 24 prediction prediction NOUN njb-55 12 25     SPACE njb-55 12 26 system system NOUN njb-55 12 27     SPACE njb-55 12 28 to to PART njb-55 12 29     SPACE njb-55 12 30 prevent prevent VERB njb-55 12 31     SPACE njb-55 12 32 disease disease NOUN njb-55 12 33     SPACE njb-55 12 34 outbreak outbreak NOUN njb-55 12 35     SPACE njb-55 12 36 by by ADP njb-55 12 37     SPACE njb-55 12 38 these these DET njb-55 12 39     SPACE njb-55 12 40 leptospira leptospira PROPN njb-55 12 41     SPACE njb-55 12 42 species specie NOUN njb-55 12 43     SPACE njb-55 12 44 and and CCONJ njb-55 12 45     SPACE njb-55 12 46 can can AUX njb-55 12 47     SPACE njb-55 12 48 be be AUX njb-55 12 49     SPACE njb-55 12 50 considered consider VERB njb-55 12 51     SPACE njb-55 12 52 as as ADP njb-55 12 53     SPACE njb-55 12 54 an an DET njb-55 12 55     SPACE njb-55 12 56 effective effective ADJ njb-55 12 57     SPACE njb-55 12 58 tool tool NOUN njb-55 12 59     SPACE njb-55 12 60 for for ADP njb-55 12 61     SPACE njb-55 12 62 disease disease NOUN njb-55 12 63     SPACE njb-55 12 64 diagnosis diagnosis NOUN njb-55 12 65     SPACE njb-55 12 66 of of ADP njb-55 12 67     SPACE njb-55 12 68 leptospira leptospira PROPN njb-55 12 69     SPACE njb-55 12 70 species specie NOUN njb-55 12 71 . . PUNCT njb-55 12 72         SPACE njb-55 12 73 key key ADJ njb-55 12 74     SPACE njb-55 12 75 words word NOUN njb-55 12 76 : : PUNCT njb-55 12 77     SPACE njb-55 12 78 pcr pcr ADJ njb-55 12 79 , , PUNCT njb-55 12 80     SPACE njb-55 12 81 lipl21 lipl21 NOUN njb-55 12 82 , , PUNCT njb-55 12 83     SPACE njb-55 12 84 lipl41 lipl41 PROPN njb-55 12 85 , , PUNCT njb-55 12 86     SPACE njb-55 12 87 molecular molecular ADJ njb-55 12 88     SPACE njb-55 12 89 diagnostics diagnostic NOUN njb-55 12 90 , , PUNCT njb-55 12 91     SPACE njb-55 12 92 transformation transformation NOUN njb-55 12 93 . . PUNCT njb-55 12 94           SPACE njb-55 13 1 correspondence correspondence NOUN njb-55 13 2     SPACE njb-55 13 3 author author NOUN njb-55 13 4 : : PUNCT njb-55 13 5       SPACE njb-55 13 6 sharanaiah sharanaiah PROPN njb-55 13 7     SPACE njb-55 13 8 umesha umesha PROPN njb-55 13 9 , , PUNCT njb-55 13 10     SPACE njb-55 13 11 dos do NOUN njb-55 13 12     SPACE njb-55 13 13 in in ADP njb-55 13 14     SPACE njb-55 13 15 biotechnology biotechnology NOUN njb-55 13 16 , , PUNCT njb-55 13 17     SPACE njb-55 13 18 university university NOUN njb-55 13 19     SPACE njb-55 13 20 of of ADP njb-55 13 21     SPACE njb-55 13 22 mysore mysore NOUN njb-55 13 23 , , PUNCT njb-55 13 24     SPACE njb-55 13 25 karnataka karnataka PROPN njb-55 13 26 , , PUNCT njb-55 13 27     SPACE njb-55 13 28 india india PROPN njb-55 13 29 . . PUNCT njb-55 13 30     SPACE njb-55 13 31 e‐mail e‐mail NOUN njb-55 13 32 : : PUNCT njb-55 13 33     SPACE njb-55 13 34 umeshgroup@yahoo.co.in umeshgroup@yahoo.co.in VERB njb-55 13 35     SPACE njb-55 13 36 introduction introduction NOUN njb-55 13 37     SPACE njb-55 13 38 the the DET njb-55 13 39     SPACE njb-55 13 40 economic economic ADJ njb-55 13 41     SPACE njb-55 13 42 importance importance NOUN njb-55 13 43     SPACE njb-55 13 44 of of ADP njb-55 13 45     SPACE njb-55 13 46 bovine bovine NOUN njb-55 13 47     SPACE njb-55 13 48 leptospirosis leptospirosis NOUN njb-55 13 49     SPACE njb-55 13 50 includes include VERB njb-55 13 51     SPACE njb-55 13 52 loss loss NOUN njb-55 13 53     SPACE njb-55 13 54 due due ADJ njb-55 13 55     SPACE njb-55 13 56 to to ADP njb-55 13 57     SPACE njb-55 13 58 abortion abortion NOUN njb-55 13 59 , , PUNCT njb-55 13 60     SPACE njb-55 13 61 loss loss NOUN njb-55 13 62     SPACE njb-55 13 63 of of ADP njb-55 13 64     SPACE njb-55 13 65 milk milk NOUN njb-55 13 66     SPACE njb-55 13 67 production production NOUN njb-55 13 68     SPACE njb-55 13 69 and and CCONJ njb-55 13 70     SPACE njb-55 13 71 elevated elevate VERB njb-55 13 72     SPACE njb-55 13 73 veterinary veterinary ADJ njb-55 13 74     SPACE njb-55 13 75 costs cost NOUN njb-55 13 76     SPACE njb-55 13 77 and and CCONJ njb-55 13 78     SPACE njb-55 13 79 predominantly predominantly ADV njb-55 13 80     SPACE njb-55 13 81 human human ADJ njb-55 13 82     SPACE njb-55 13 83 infection infection NOUN njb-55 13 84     SPACE njb-55 13 85 ( ( PUNCT njb-55 13 86 levett levett PROPN njb-55 13 87     SPACE njb-55 13 88 et et PROPN njb-55 13 89     SPACE njb-55 13 90 al al PROPN njb-55 13 91 . . PROPN njb-55 13 92 , , PUNCT njb-55 13 93     SPACE njb-55 13 94 2005 2005 NUM njb-55 13 95 ) ) PUNCT njb-55 13 96 . . PUNCT njb-55 13 97         SPACE njb-55 14 1 leptospirosis leptospirosis NOUN njb-55 14 2     SPACE njb-55 14 3 also also ADV njb-55 14 4     SPACE njb-55 14 5 known know VERB njb-55 14 6     SPACE njb-55 14 7 as as ADP njb-55 14 8     SPACE njb-55 14 9 weil weil PROPN njb-55 14 10 's 's PART njb-55 14 11     SPACE njb-55 14 12 disease disease NOUN njb-55 14 13 , , PUNCT njb-55 14 14     SPACE njb-55 14 15 canicola canicola PROPN njb-55 14 16     SPACE njb-55 14 17 fever fever NOUN njb-55 14 18 , , PUNCT njb-55 14 19     SPACE njb-55 14 20 canefield canefield PROPN njb-55 14 21     SPACE njb-55 14 22 fever fever NOUN njb-55 14 23 , , PUNCT njb-55 14 24     SPACE njb-55 14 25 nanukayami nanukayami NOUN njb-55 14 26     SPACE njb-55 14 27 fever fever NOUN njb-55 14 28 , , PUNCT njb-55 14 29     SPACE njb-55 14 30 7‐day 7‐day NOUN njb-55 14 31     SPACE njb-55 14 32 fever fever NOUN njb-55 14 33     SPACE njb-55 14 34 and and CCONJ njb-55 14 35     SPACE njb-55 14 36 many many ADJ njb-55 14 37     SPACE njb-55 14 38 more more ADJ njb-55 14 39 . . PUNCT njb-55 14 40       SPACE njb-55 15 1 leptospirosis leptospirosis NOUN njb-55 15 2     SPACE njb-55 15 3 recognized recognize VERB njb-55 15 4     SPACE njb-55 15 5 as as ADP njb-55 15 6     SPACE njb-55 15 7 one one NUM njb-55 15 8     SPACE njb-55 15 9 of of ADP njb-55 15 10     SPACE njb-55 15 11 the the DET njb-55 15 12     SPACE njb-55 15 13 most most ADJ njb-55 15 14     SPACE njb-55 15 15 common common ADJ njb-55 15 16     SPACE njb-55 15 17 zoonoses zoonosis NOUN njb-55 15 18 . . PUNCT njb-55 15 19     SPACE njb-55 16 1 leptospirosis leptospirosis NOUN njb-55 16 2     SPACE njb-55 16 3 is be AUX njb-55 16 4     SPACE njb-55 16 5 commonly commonly ADV njb-55 16 6     SPACE njb-55 16 7 transmitted transmit VERB njb-55 16 8     SPACE njb-55 16 9 to to ADP njb-55 16 10     SPACE njb-55 16 11 humans human NOUN njb-55 16 12     SPACE njb-55 16 13 through through ADP njb-55 16 14     SPACE njb-55 16 15 contact contact NOUN njb-55 16 16     SPACE njb-55 16 17 of of ADP njb-55 16 18     SPACE njb-55 16 19 animal animal NOUN njb-55 16 20     SPACE njb-55 16 21 urine urine NOUN njb-55 16 22     SPACE njb-55 16 23 with with ADP njb-55 16 24     SPACE njb-55 16 25 unhealed unheale VERB njb-55 16 26     SPACE njb-55 16 27 breaks break NOUN njb-55 16 28     SPACE njb-55 16 29 in in ADP njb-55 16 30     SPACE njb-55 16 31 the the DET njb-55 16 32     SPACE njb-55 16 33 skin skin NOUN njb-55 16 34 , , PUNCT njb-55 16 35     SPACE njb-55 16 36 eyes eye NOUN njb-55 16 37     SPACE njb-55 16 38 or or CCONJ njb-55 16 39     SPACE njb-55 16 40 with with ADP njb-55 16 41     SPACE njb-55 16 42 the the DET njb-55 16 43     SPACE njb-55 16 44 mucous mucous ADJ njb-55 16 45     SPACE njb-55 16 46 membranes membrane NOUN njb-55 16 47 . . PUNCT njb-55 16 48     SPACE njb-55 17 1 outside outside ADP njb-55 17 2     SPACE njb-55 17 3 tropical tropical ADJ njb-55 17 4     SPACE njb-55 17 5 areas area NOUN njb-55 17 6 , , PUNCT njb-55 17 7     SPACE njb-55 17 8 leptospirosis leptospirosis NOUN njb-55 17 9     SPACE njb-55 17 10 cases case NOUN njb-55 17 11     SPACE njb-55 17 12 have have VERB njb-55 17 13     SPACE njb-55 17 14 a a DET njb-55 17 15     SPACE njb-55 17 16 relatively relatively ADV njb-55 17 17     SPACE njb-55 17 18 distinct distinct ADJ njb-55 17 19     SPACE njb-55 17 20 seasonality seasonality NOUN njb-55 17 21     SPACE njb-55 17 22 with with ADP njb-55 17 23     SPACE njb-55 17 24 most most ADJ njb-55 17 25     SPACE njb-55 17 26 of of ADP njb-55 17 27     SPACE njb-55 17 28 them they PRON njb-55 17 29     SPACE njb-55 17 30 occurring occur VERB njb-55 17 31     SPACE njb-55 17 32 during during ADP njb-55 17 33     SPACE njb-55 17 34 august‐september august‐september NOUN njb-55 17 35 / / SYM njb-55 17 36 february‐march february‐march PROPN njb-55 17 37     SPACE njb-55 17 38 in in ADP njb-55 17 39       SPACE njb-55 17 40 year year NOUN njb-55 17 41     SPACE njb-55 17 42 ( ( PUNCT njb-55 17 43 zhang zhang PROPN njb-55 17 44     SPACE njb-55 17 45 et et PROPN njb-55 17 46     SPACE njb-55 17 47 al al PROPN njb-55 17 48 . . PROPN njb-55 17 49 , , PUNCT njb-55 17 50     SPACE njb-55 17 51 1992 1992 NUM njb-55 17 52 ) ) PUNCT njb-55 17 53 . . PUNCT njb-55 17 54       SPACE njb-55 18 1 the the DET njb-55 18 2     SPACE njb-55 18 3 spectrum spectrum NOUN njb-55 18 4     SPACE njb-55 18 5 of of ADP njb-55 18 6     SPACE njb-55 18 7 human human NOUN njb-55 18 8     SPACE njb-55 18 9 disease disease NOUN njb-55 18 10     SPACE njb-55 18 11 caused cause VERB njb-55 18 12     SPACE njb-55 18 13 by by ADP njb-55 18 14     SPACE njb-55 18 15 leptospires leptospire NOUN njb-55 18 16     SPACE njb-55 18 17 is be AUX njb-55 18 18     SPACE njb-55 18 19 extremely extremely ADV njb-55 18 20     SPACE njb-55 18 21 wide wide ADJ njb-55 18 22 , , PUNCT njb-55 18 23     SPACE njb-55 18 24 ranging range VERB njb-55 18 25     SPACE njb-55 18 26 from from ADP njb-55 18 27     SPACE njb-55 18 28 subclinical subclinical ADJ njb-55 18 29     SPACE njb-55 18 30 infection infection NOUN njb-55 18 31     SPACE njb-55 18 32 to to ADP njb-55 18 33     SPACE njb-55 18 34 a a DET njb-55 18 35     SPACE njb-55 18 36 severe severe ADJ njb-55 18 37     SPACE njb-55 18 38 syndrome syndrome NOUN njb-55 18 39     SPACE njb-55 18 40 of of ADP njb-55 18 41     SPACE njb-55 18 42 multi multi NOUN njb-55 18 43     SPACE njb-55 18 44 organ organ NOUN njb-55 18 45     SPACE njb-55 18 46 infection infection NOUN njb-55 18 47     SPACE njb-55 18 48 resulting result VERB njb-55 18 49     SPACE njb-55 18 50 high high ADJ njb-55 18 51     SPACE njb-55 18 52 mortality mortality NOUN njb-55 18 53 . . PUNCT njb-55 18 54     SPACE njb-55 19 1 this this DET njb-55 19 2     SPACE njb-55 19 3 syndrome syndrome NOUN njb-55 19 4 , , PUNCT njb-55 19 5     SPACE njb-55 19 6 icteric icteric ADJ njb-55 19 7     SPACE njb-55 19 8 leptospirosis leptospirosis NOUN njb-55 19 9     SPACE njb-55 19 10 with with ADP njb-55 19 11     SPACE njb-55 19 12 renal renal ADJ njb-55 19 13     SPACE njb-55 19 14 failure failure NOUN njb-55 19 15 , , PUNCT njb-55 19 16     SPACE njb-55 19 17 was be AUX njb-55 19 18     SPACE njb-55 19 19 first first ADJ njb-55 19 20     SPACE njb-55 19 21 reported report VERB njb-55 19 22     SPACE njb-55 19 23 more more ADJ njb-55 19 24     SPACE njb-55 19 25 than than ADP njb-55 19 26     SPACE njb-55 19 27 100 100 NUM njb-55 19 28     SPACE njb-55 19 29 years year NOUN njb-55 19 30     SPACE njb-55 19 31 ago ago ADV njb-55 19 32     SPACE njb-55 19 33 by by ADP njb-55 19 34     SPACE njb-55 19 35 adolf adolf PROPN njb-55 19 36     SPACE njb-55 19 37 weil weil PROPN njb-55 19 38     SPACE njb-55 19 39 in in ADP njb-55 19 40     SPACE njb-55 19 41 heidelberg heidelberg PROPN njb-55 19 42 . . PUNCT njb-55 19 43     SPACE njb-55 19 44 however however ADV njb-55 19 45 , , PUNCT njb-55 19 46     SPACE njb-55 19 47 an an DET njb-55 19 48     SPACE njb-55 19 49 apparently apparently ADV njb-55 19 50     SPACE njb-55 19 51 identical identical ADJ njb-55 19 52     SPACE njb-55 19 53 syndrome syndrome NOUN njb-55 19 54     SPACE njb-55 19 55 occurring occur VERB njb-55 19 56     SPACE njb-55 19 57 in in ADP njb-55 19 58     SPACE njb-55 19 59 sewer sewer NOUN njb-55 19 60     SPACE njb-55 19 61 workers worker NOUN njb-55 19 62     SPACE njb-55 19 63 was be AUX njb-55 19 64     SPACE njb-55 19 65 described describe VERB njb-55 19 66     SPACE njb-55 19 67 several several ADJ njb-55 19 68     SPACE njb-55 19 69 years year NOUN njb-55 19 70     SPACE njb-55 19 71 earlier early ADV njb-55 19 72 . . PUNCT njb-55 19 73     SPACE njb-55 20 1 earlier early ADJ njb-55 20 2     SPACE njb-55 20 3 descriptions description NOUN njb-55 20 4     SPACE njb-55 20 5 of of ADP njb-55 20 6     SPACE njb-55 20 7 diseases disease NOUN njb-55 20 8     SPACE njb-55 20 9 that that PRON njb-55 20 10     SPACE njb-55 20 11 were be AUX njb-55 20 12     SPACE njb-55 20 13 probably probably ADV njb-55 20 14     SPACE njb-55 20 15 leptospirosis leptospirosis NOUN njb-55 20 16     SPACE njb-55 20 17 were be AUX njb-55 20 18     SPACE njb-55 20 19 reviewed review VERB njb-55 20 20     SPACE njb-55 20 21 recently recently ADV njb-55 20 22     SPACE njb-55 20 23 ( ( PUNCT njb-55 20 24 serres serre NOUN njb-55 20 25     SPACE njb-55 20 26 et et NOUN njb-55 20 27     SPACE njb-55 20 28 al al PROPN njb-55 20 29 . . PROPN njb-55 20 30 , , PUNCT njb-55 20 31     SPACE njb-55 20 32 1995 1995 NUM njb-55 20 33 ) ) PUNCT njb-55 20 34 . . PUNCT njb-55 20 35     SPACE njb-55 21 1 leptospira leptospira PROPN njb-55 21 2     SPACE njb-55 21 3 ( ( PUNCT njb-55 21 4 from from ADP njb-55 21 5     SPACE njb-55 21 6 the the DET njb-55 21 7     SPACE njb-55 21 8 greek greek ADJ njb-55 21 9     SPACE njb-55 21 10 word word NOUN njb-55 21 11     SPACE njb-55 21 12 leptos lepto NOUN njb-55 21 13     SPACE njb-55 21 14 means mean VERB njb-55 21 15     SPACE njb-55 21 16 fine fine ADJ njb-55 21 17     SPACE njb-55 21 18 or or CCONJ njb-55 21 19     SPACE njb-55 21 20 thin thin ADJ njb-55 21 21     SPACE njb-55 21 22 and and CCONJ njb-55 21 23     SPACE njb-55 21 24 latin latin NOUN njb-55 21 25     SPACE njb-55 21 26 word word NOUN njb-55 21 27     SPACE njb-55 21 28 spira spira PROPN njb-55 21 29     SPACE njb-55 21 30 means mean VERB njb-55 21 31     SPACE njb-55 21 32 saprophytic saprophytic ADJ njb-55 21 33     SPACE njb-55 21 34 species specie NOUN njb-55 21 35 ) ) PUNCT njb-55 21 36 . . PUNCT njb-55 21 37     SPACE njb-55 22 1 leptospira leptospira PROPN njb-55 22 2     SPACE njb-55 22 3 was be AUX njb-55 22 4     SPACE njb-55 22 5 first first ADJ njb-55 22 6     SPACE njb-55 22 7 observed observe VERB njb-55 22 8     SPACE njb-55 22 9 in in ADP njb-55 22 10     SPACE njb-55 22 11 1907 1907 NUM njb-55 22 12     SPACE njb-55 22 13 in in ADP njb-55 22 14     SPACE njb-55 22 15 kidney kidney NOUN njb-55 22 16     SPACE njb-55 22 17 tissue tissue NOUN njb-55 22 18     SPACE njb-55 22 19 slices slice NOUN njb-55 22 20     SPACE njb-55 22 21 of of ADP njb-55 22 22     SPACE njb-55 22 23 a a DET njb-55 22 24     SPACE njb-55 22 25 leptospirosis leptospirosis NOUN njb-55 22 26     SPACE njb-55 22 27 victim victim NOUN njb-55 22 28     SPACE njb-55 22 29 who who PRON njb-55 22 30     SPACE njb-55 22 31 was be AUX njb-55 22 32     SPACE njb-55 22 33 reported report VERB njb-55 22 34     SPACE njb-55 22 35 to to PART njb-55 22 36     SPACE njb-55 22 37 die die VERB njb-55 22 38     SPACE njb-55 22 39 of of ADP njb-55 22 40     SPACE njb-55 22 41 “ " PUNCT njb-55 22 42 yellow yellow ADJ njb-55 22 43     SPACE njb-55 22 44 fever fever NOUN njb-55 22 45 ” " PUNCT njb-55 22 46     SPACE njb-55 22 47 ( ( PUNCT njb-55 22 48 abdollahpour abdollahpour PROPN njb-55 22 49 , , PUNCT njb-55 22 50     SPACE njb-55 22 51 1990 1990 NUM njb-55 22 52 ) ) PUNCT njb-55 22 53 . . PUNCT njb-55 22 54               SPACE njb-55 23 1 the the DET njb-55 23 2     SPACE njb-55 23 3 direct direct ADJ njb-55 23 4     SPACE njb-55 23 5 nepal nepal ADJ njb-55 23 6     SPACE njb-55 23 7 journal journal NOUN njb-55 23 8     SPACE njb-55 23 9 of of ADP njb-55 23 10     SPACE njb-55 23 11 biotechnology biotechnology NOUN njb-55 23 12 . . PUNCT njb-55 23 13     SPACE njb-55 24 1 jan jan PROPN njb-55 24 2 . . PROPN njb-55 24 3     SPACE njb-55 24 4 2011 2011 NUM njb-55 24 5 , , PUNCT njb-55 24 6     SPACE njb-55 24 7 vol vol NOUN njb-55 24 8 . . PUNCT njb-55 24 9     SPACE njb-55 24 10 1 1 NUM njb-55 24 11 , , PUNCT njb-55 24 12     SPACE njb-55 24 13 no no INTJ njb-55 24 14 . . PUNCT njb-55 24 15     SPACE njb-55 24 16 1 1 NUM njb-55 24 17 : : PUNCT njb-55 24 18     SPACE njb-55 24 19 22‐30 22‐30 NUM njb-55 24 20     SPACE njb-55 24 21 23 23 NUM njb-55 24 22     SPACE njb-55 24 23 biotechnology biotechnology NOUN njb-55 24 24     SPACE njb-55 24 25 society society NOUN njb-55 24 26     SPACE njb-55 24 27 of of ADP njb-55 24 28     SPACE njb-55 24 29 nepal nepal NOUN njb-55 24 30     SPACE njb-55 24 31 ( ( PUNCT njb-55 24 32 bsn bsn PROPN njb-55 24 33 ) ) PUNCT njb-55 24 34 , , PUNCT njb-55 24 35     SPACE njb-55 24 36 all all DET njb-55 24 37     SPACE njb-55 24 38 rights right NOUN njb-55 24 39     SPACE njb-55 24 40 reserved reserve VERB njb-55 24 41       SPACE njb-55 24 42 in in ADP njb-55 24 43     SPACE njb-55 24 44 artificial artificial ADJ njb-55 24 45     SPACE njb-55 24 46 medium medium NOUN njb-55 24 47 , , PUNCT njb-55 24 48     SPACE njb-55 24 49 with with ADP njb-55 24 50     SPACE njb-55 24 51 leptospira leptospira PROPN njb-55 24 52     SPACE njb-55 24 53 present present NOUN njb-55 24 54     SPACE njb-55 24 55 in in ADP njb-55 24 56     SPACE njb-55 24 57 an an DET njb-55 24 58     SPACE njb-55 24 59 infected infect VERB njb-55 24 60     SPACE njb-55 24 61 animal animal NOUN njb-55 24 62 . . PUNCT njb-55 24 63     SPACE njb-55 25 1 several several ADJ njb-55 25 2     SPACE njb-55 25 3 leptospiral leptospiral ADJ njb-55 25 4     SPACE njb-55 25 5 outer outer ADJ njb-55 25 6     SPACE njb-55 25 7 membrane membrane NOUN njb-55 25 8     SPACE njb-55 25 9 proteins protein NOUN njb-55 25 10     SPACE njb-55 25 11 have have AUX njb-55 25 12     SPACE njb-55 25 13 been be AUX njb-55 25 14     SPACE njb-55 25 15 shown show VERB njb-55 25 16     SPACE njb-55 25 17 to to PART njb-55 25 18     SPACE njb-55 25 19 attach attach VERB njb-55 25 20     SPACE njb-55 25 21 to to ADP njb-55 25 22     SPACE njb-55 25 23 the the DET njb-55 25 24     SPACE njb-55 25 25 host host NOUN njb-55 25 26     SPACE njb-55 25 27 extracellular extracellular ADJ njb-55 25 28     SPACE njb-55 25 29 matrix matrix NOUN njb-55 25 30     SPACE njb-55 25 31 and and CCONJ njb-55 25 32     SPACE njb-55 25 33 to to PART njb-55 25 34     SPACE njb-55 25 35 factor factor NOUN njb-55 25 36     SPACE njb-55 25 37 h h PROPN njb-55 25 38 , , PUNCT njb-55 25 39     SPACE njb-55 25 40 suggesting suggest VERB njb-55 25 41     SPACE njb-55 25 42 these these DET njb-55 25 43     SPACE njb-55 25 44 proteins protein NOUN njb-55 25 45     SPACE njb-55 25 46 may may AUX njb-55 25 47     SPACE njb-55 25 48 be be AUX njb-55 25 49     SPACE njb-55 25 50 important important ADJ njb-55 25 51     SPACE njb-55 25 52 for for ADP njb-55 25 53     SPACE njb-55 25 54 adhesion adhesion NOUN njb-55 25 55     SPACE njb-55 25 56 of of ADP njb-55 25 57     SPACE njb-55 25 58 leptospira leptospira PROPN njb-55 25 59     SPACE njb-55 25 60 to to PART njb-55 25 61     SPACE njb-55 25 62 host host NOUN njb-55 25 63     SPACE njb-55 25 64 tissues tissue NOUN njb-55 25 65     SPACE njb-55 25 66 and and CCONJ njb-55 25 67     SPACE njb-55 25 68 in in ADP njb-55 25 69     SPACE njb-55 25 70 resisting resist VERB njb-55 25 71     SPACE njb-55 25 72 complement complement NOUN njb-55 25 73 , , PUNCT njb-55 25 74     SPACE njb-55 25 75 respectively respectively ADV njb-55 25 76 . . PUNCT njb-55 25 77     SPACE njb-55 26 1 the the DET njb-55 26 2     SPACE njb-55 26 3 outer outer ADJ njb-55 26 4     SPACE njb-55 26 5 membrane membrane NOUN njb-55 26 6     SPACE njb-55 26 7 of of ADP njb-55 26 8     SPACE njb-55 26 9 leptospira leptospira PROPN njb-55 26 10 , , PUNCT njb-55 26 11     SPACE njb-55 26 12 like like ADP njb-55 26 13     SPACE njb-55 26 14 those those PRON njb-55 26 15     SPACE njb-55 26 16 of of ADP njb-55 26 17     SPACE njb-55 26 18 most most ADJ njb-55 26 19     SPACE njb-55 26 20 other other ADJ njb-55 26 21     SPACE njb-55 26 22 gram‐negative gram‐negative NOUN njb-55 26 23     SPACE njb-55 26 24 bacteria bacteria NOUN njb-55 26 25 , , PUNCT njb-55 26 26     SPACE njb-55 26 27 contains contain VERB njb-55 26 28     SPACE njb-55 26 29 lipopolysaccharide lipopolysaccharide NOUN njb-55 26 30     SPACE njb-55 26 31 ( ( PUNCT njb-55 26 32 lps lps X njb-55 26 33 ) ) PUNCT njb-55 26 34 . . PUNCT njb-55 26 35     SPACE njb-55 26 36 differences difference NOUN njb-55 26 37     SPACE njb-55 26 38 in in ADP njb-55 26 39     SPACE njb-55 26 40 the the DET njb-55 26 41     SPACE njb-55 26 42 highly highly ADV njb-55 26 43     SPACE njb-55 26 44 immunogenic immunogenic NOUN njb-55 26 45     SPACE njb-55 26 46 lps lps PROPN njb-55 26 47     SPACE njb-55 26 48 structure structure NOUN njb-55 26 49     SPACE njb-55 26 50 account account NOUN njb-55 26 51     SPACE njb-55 26 52 for for ADP njb-55 26 53     SPACE njb-55 26 54 the the DET njb-55 26 55     SPACE njb-55 26 56 numerous numerous ADJ njb-55 26 57     SPACE njb-55 26 58 serovars serovar NOUN njb-55 26 59     SPACE njb-55 26 60 of of ADP njb-55 26 61     SPACE njb-55 26 62 leptospira leptospira PROPN njb-55 26 63 . . PUNCT njb-55 26 64     SPACE njb-55 27 1 consequently consequently ADV njb-55 27 2 , , PUNCT njb-55 27 3     SPACE njb-55 27 4 immunity immunity NOUN njb-55 27 5     SPACE njb-55 27 6 is be AUX njb-55 27 7     SPACE njb-55 27 8 serovar serovar NOUN njb-55 27 9     SPACE njb-55 27 10 specific specific ADJ njb-55 27 11 ; ; PUNCT njb-55 27 12     SPACE njb-55 27 13 current current ADJ njb-55 27 14     SPACE njb-55 27 15 leptospiral leptospiral ADJ njb-55 27 16     SPACE njb-55 27 17 vaccines vaccine NOUN njb-55 27 18 , , PUNCT njb-55 27 19     SPACE njb-55 27 20 which which PRON njb-55 27 21     SPACE njb-55 27 22 consist consist VERB njb-55 27 23     SPACE njb-55 27 24 of of ADP njb-55 27 25     SPACE njb-55 27 26 one one NUM njb-55 27 27     SPACE njb-55 27 28 or or CCONJ njb-55 27 29     SPACE njb-55 27 30 several several ADJ njb-55 27 31     SPACE njb-55 27 32 serovars serovar NOUN njb-55 27 33     SPACE njb-55 27 34 of of ADP njb-55 27 35     SPACE njb-55 27 36 leptospira leptospira PROPN njb-55 27 37     SPACE njb-55 27 38 endemic endemic PROPN njb-55 27 39     SPACE njb-55 27 40 in in ADP njb-55 27 41     SPACE njb-55 27 42 the the DET njb-55 27 43     SPACE njb-55 27 44 population population NOUN njb-55 27 45     SPACE njb-55 27 46 to to PART njb-55 27 47     SPACE njb-55 27 48 be be AUX njb-55 27 49     SPACE njb-55 27 50 immunized immunize VERB njb-55 27 51 , , PUNCT njb-55 27 52     SPACE njb-55 27 53 protect protect VERB njb-55 27 54     SPACE njb-55 27 55 only only ADV njb-55 27 56     SPACE njb-55 27 57 against against ADP njb-55 27 58     SPACE njb-55 27 59 the the DET njb-55 27 60     SPACE njb-55 27 61 serovars serovar NOUN njb-55 27 62     SPACE njb-55 27 63 contained contain VERB njb-55 27 64     SPACE njb-55 27 65 in in ADP njb-55 27 66     SPACE njb-55 27 67 the the DET njb-55 27 68     SPACE njb-55 27 69 vaccine vaccine NOUN njb-55 27 70     SPACE njb-55 27 71 preparation preparation NOUN njb-55 27 72 . . PUNCT njb-55 27 73     SPACE njb-55 28 1 leptospiral leptospiral ADJ njb-55 28 2     SPACE njb-55 28 3 lps lp NOUN njb-55 28 4     SPACE njb-55 28 5 has have VERB njb-55 28 6     SPACE njb-55 28 7 low low ADJ njb-55 28 8     SPACE njb-55 28 9 endotoxin endotoxin NOUN njb-55 28 10     SPACE njb-55 28 11 activity activity NOUN njb-55 28 12 . . PUNCT njb-55 28 13       SPACE njb-55 29 1 an an DET njb-55 29 2     SPACE njb-55 29 3 unusual unusual ADJ njb-55 29 4     SPACE njb-55 29 5 feature feature NOUN njb-55 29 6     SPACE njb-55 29 7 of of ADP njb-55 29 8     SPACE njb-55 29 9 leptospiral leptospiral ADJ njb-55 29 10     SPACE njb-55 29 11 lps lp NOUN njb-55 29 12     SPACE njb-55 29 13 is be AUX njb-55 29 14     SPACE njb-55 29 15 that that SCONJ njb-55 29 16     SPACE njb-55 29 17 it it PRON njb-55 29 18     SPACE njb-55 29 19 activates activate VERB njb-55 29 20     SPACE njb-55 29 21 host host NOUN njb-55 29 22     SPACE njb-55 29 23 cells cell NOUN njb-55 29 24     SPACE njb-55 29 25 via via ADP njb-55 29 26     SPACE njb-55 29 27 tlr2 tlr2 PROPN njb-55 29 28     SPACE njb-55 29 29 rather rather ADV njb-55 29 30     SPACE njb-55 29 31 than than ADP njb-55 29 32     SPACE njb-55 29 33 tlr4 tlr4 PROPN njb-55 29 34 . . PUNCT njb-55 29 35         SPACE njb-55 30 1 the the DET njb-55 30 2     SPACE njb-55 30 3 unique unique ADJ njb-55 30 4     SPACE njb-55 30 5 structure structure NOUN njb-55 30 6     SPACE njb-55 30 7 of of ADP njb-55 30 8     SPACE njb-55 30 9 the the DET njb-55 30 10     SPACE njb-55 30 11 lipid lipid NOUN njb-55 30 12     SPACE njb-55 30 13 a a DET njb-55 30 14     SPACE njb-55 30 15 portion portion NOUN njb-55 30 16     SPACE njb-55 30 17 of of ADP njb-55 30 18     SPACE njb-55 30 19 the the DET njb-55 30 20     SPACE njb-55 30 21 lps lps PROPN njb-55 30 22     SPACE njb-55 30 23 molecule molecule NOUN njb-55 30 24     SPACE njb-55 30 25 may may AUX njb-55 30 26     SPACE njb-55 30 27 account account VERB njb-55 30 28     SPACE njb-55 30 29 for for ADP njb-55 30 30     SPACE njb-55 30 31 this this DET njb-55 30 32     SPACE njb-55 30 33 observation observation NOUN njb-55 30 34 . . PUNCT njb-55 30 35     SPACE njb-55 31 1 finally finally ADV njb-55 31 2 , , PUNCT njb-55 31 3     SPACE njb-55 31 4 the the DET njb-55 31 5     SPACE njb-55 31 6 lps lps X njb-55 31 7     SPACE njb-55 31 8 o o PROPN njb-55 31 9     SPACE njb-55 31 10 antigen antigen NOUN njb-55 31 11     SPACE njb-55 31 12 content content NOUN njb-55 31 13     SPACE njb-55 31 14 of of ADP njb-55 31 15     SPACE njb-55 31 16 l. l. PROPN njb-55 31 17     SPACE njb-55 31 18 interrogans interrogan NOUN njb-55 31 19     SPACE njb-55 31 20 differs differ VERB njb-55 31 21     SPACE njb-55 31 22 in in ADP njb-55 31 23     SPACE njb-55 31 24 an an DET njb-55 31 25     SPACE njb-55 31 26 acutely acutely ADV njb-55 31 27     SPACE njb-55 31 28 infected infect VERB njb-55 31 29     SPACE njb-55 31 30 versus versus ADP njb-55 31 31     SPACE njb-55 31 32 a a DET njb-55 31 33     SPACE njb-55 31 34 chronically chronically ADV njb-55 31 35     SPACE njb-55 31 36 infected infect VERB njb-55 31 37     SPACE njb-55 31 38 animal animal NOUN njb-55 31 39 . . PUNCT njb-55 31 40       SPACE njb-55 32 1 the the DET njb-55 32 2     SPACE njb-55 32 3 role role NOUN njb-55 32 4     SPACE njb-55 32 5 of of ADP njb-55 32 6     SPACE njb-55 32 7 o o PROPN njb-55 32 8     SPACE njb-55 32 9 antigen antigen NOUN njb-55 32 10     SPACE njb-55 32 11 changes change NOUN njb-55 32 12     SPACE njb-55 32 13 in in ADP njb-55 32 14     SPACE njb-55 32 15 the the DET njb-55 32 16     SPACE njb-55 32 17 establishment establishment NOUN njb-55 32 18     SPACE njb-55 32 19 or or CCONJ njb-55 32 20     SPACE njb-55 32 21 maintenance maintenance NOUN njb-55 32 22     SPACE njb-55 32 23 of of ADP njb-55 32 24     SPACE njb-55 32 25 acute acute ADJ njb-55 32 26     SPACE njb-55 32 27 or or CCONJ njb-55 32 28     SPACE njb-55 32 29 chronic chronic ADJ njb-55 32 30     SPACE njb-55 32 31 infection infection NOUN njb-55 32 32 , , PUNCT njb-55 32 33     SPACE njb-55 32 34 if if SCONJ njb-55 32 35     SPACE njb-55 32 36 any any PRON njb-55 32 37 , , PUNCT njb-55 32 38     SPACE njb-55 32 39 is be AUX njb-55 32 40     SPACE njb-55 32 41 unknown unknown ADJ njb-55 32 42     SPACE njb-55 32 43 ( ( PUNCT njb-55 32 44 zhang zhang PROPN njb-55 32 45     SPACE njb-55 32 46 et et PROPN njb-55 32 47     SPACE njb-55 32 48 al al PROPN njb-55 32 49 , , PUNCT njb-55 32 50 . . PUNCT njb-55 32 51     SPACE njb-55 32 52 1992 1992 NUM njb-55 32 53 ; ; PUNCT njb-55 32 54     SPACE njb-55 32 55 bharti bharti VERB njb-55 32 56     SPACE njb-55 32 57 et et PROPN njb-55 32 58     SPACE njb-55 32 59 al al PROPN njb-55 32 60 . . PROPN njb-55 32 61 , , PUNCT njb-55 32 62     SPACE njb-55 32 63 2003 2003 NUM njb-55 32 64 ) ) PUNCT njb-55 32 65 . . PUNCT njb-55 32 66     SPACE njb-55 32 67 leptospires leptospire NOUN njb-55 32 68     SPACE njb-55 32 69 enter enter VERB njb-55 32 70     SPACE njb-55 32 71 into into ADP njb-55 32 72     SPACE njb-55 32 73 the the DET njb-55 32 74     SPACE njb-55 32 75 body body NOUN njb-55 32 76     SPACE njb-55 32 77 of of ADP njb-55 32 78     SPACE njb-55 32 79 a a DET njb-55 32 80     SPACE njb-55 32 81 susceptible susceptible ADJ njb-55 32 82     SPACE njb-55 32 83 host host NOUN njb-55 32 84     SPACE njb-55 32 85 through through ADP njb-55 32 86     SPACE njb-55 32 87 mucous mucous ADJ njb-55 32 88     SPACE njb-55 32 89 membrane membrane NOUN njb-55 32 90     SPACE njb-55 32 91 or or CCONJ njb-55 32 92     SPACE njb-55 32 93 abraded abrade VERB njb-55 32 94     SPACE njb-55 32 95 skin skin NOUN njb-55 32 96 . . PUNCT njb-55 32 97         SPACE njb-55 33 1 after after ADP njb-55 33 2     SPACE njb-55 33 3 4 4 NUM njb-55 33 4     SPACE njb-55 33 5 to to ADP njb-55 33 6     SPACE njb-55 33 7 10 10 NUM njb-55 33 8     SPACE njb-55 33 9 days day NOUN njb-55 33 10 , , PUNCT njb-55 33 11     SPACE njb-55 33 12 the the DET njb-55 33 13     SPACE njb-55 33 14 host host NOUN njb-55 33 15     SPACE njb-55 33 16 becomes become VERB njb-55 33 17     SPACE njb-55 33 18 bacteraemic bacteraemic ADJ njb-55 33 19 , , PUNCT njb-55 33 20     SPACE njb-55 33 21 this this DET njb-55 33 22     SPACE njb-55 33 23 period period NOUN njb-55 33 24     SPACE njb-55 33 25 lasting last VERB njb-55 33 26     SPACE njb-55 33 27 from from ADP njb-55 33 28     SPACE njb-55 33 29 hours hour NOUN njb-55 33 30     SPACE njb-55 33 31 to to ADP njb-55 33 32     SPACE njb-55 33 33 7 7 NUM njb-55 33 34     SPACE njb-55 33 35 days day NOUN njb-55 33 36 , , PUNCT njb-55 33 37     SPACE njb-55 33 38 and and CCONJ njb-55 33 39     SPACE njb-55 33 40 may may AUX njb-55 33 41     SPACE njb-55 33 42 be be AUX njb-55 33 43     SPACE njb-55 33 44 characterised characterise VERB njb-55 33 45     SPACE njb-55 33 46 by by ADP njb-55 33 47     SPACE njb-55 33 48 pyrexia pyrexia NOUN njb-55 33 49     SPACE njb-55 33 50 and and CCONJ njb-55 33 51     SPACE njb-55 33 52 anorexia anorexia NOUN njb-55 33 53     SPACE njb-55 33 54 ( ( PUNCT njb-55 33 55 abdollahpour abdollahpour PROPN njb-55 33 56 , , PUNCT njb-55 33 57     SPACE njb-55 33 58 1990 1990 NUM njb-55 33 59 ) ) PUNCT njb-55 33 60 . . PUNCT njb-55 33 61       SPACE njb-55 34 1 materials material NOUN njb-55 34 2     SPACE njb-55 34 3 and and CCONJ njb-55 34 4     SPACE njb-55 34 5 methods method NOUN njb-55 34 6     SPACE njb-55 34 7 leptospira leptospira PROPN njb-55 34 8     SPACE njb-55 34 9 serovars serovar NOUN njb-55 34 10 . . PUNCT njb-55 34 11     SPACE njb-55 35 1 a a DET njb-55 35 2     SPACE njb-55 35 3 total total NOUN njb-55 35 4     SPACE njb-55 35 5 of of ADP njb-55 35 6     SPACE njb-55 35 7 9 9 NUM njb-55 35 8     SPACE njb-55 35 9 leptospira leptospira PROPN njb-55 35 10     SPACE njb-55 35 11 serovars serovar NOUN njb-55 35 12     SPACE njb-55 35 13 namely namely ADV njb-55 35 14 , , PUNCT njb-55 35 15     SPACE njb-55 35 16 l. l. NOUN njb-55 35 17     SPACE njb-55 35 18 icterohaemorrhagiae icterohaemorrhagiae NOUN njb-55 35 19 , , PUNCT njb-55 35 20     SPACE njb-55 35 21 l. l. PROPN njb-55 35 22     SPACE njb-55 35 23 canicola canicola PROPN njb-55 35 24 , , PUNCT njb-55 35 25     SPACE njb-55 35 26 l. l. PROPN njb-55 35 27     SPACE njb-55 35 28 pamona pamona PROPN njb-55 35 29 , , PUNCT njb-55 35 30     SPACE njb-55 35 31 l. l. PROPN njb-55 35 32     SPACE njb-55 35 33 autumnalis autumnalis PROPN njb-55 35 34 , , PUNCT njb-55 35 35     SPACE njb-55 35 36 l. l. PROPN njb-55 35 37     SPACE njb-55 35 38 javanica javanica PROPN njb-55 35 39 , , PUNCT njb-55 35 40     SPACE njb-55 35 41 l. l. PROPN njb-55 35 42     SPACE njb-55 35 43 pyrogenes pyrogene NOUN njb-55 35 44 , , PUNCT njb-55 35 45     SPACE njb-55 35 46 l. l. PROPN njb-55 35 47     SPACE njb-55 35 48 australis australi NOUN njb-55 35 49 , , PUNCT njb-55 35 50     SPACE njb-55 35 51 l. l. PROPN njb-55 35 52     SPACE njb-55 35 53 hardjo hardjo PROPN njb-55 35 54     SPACE njb-55 35 55 and and CCONJ njb-55 35 56     SPACE njb-55 35 57 l. l. PROPN njb-55 35 58     SPACE njb-55 35 59 inadai inadai PROPN njb-55 35 60     SPACE njb-55 35 61 were be AUX njb-55 35 62     SPACE njb-55 35 63 procured procure VERB njb-55 35 64     SPACE njb-55 35 65 from from ADP njb-55 35 66     SPACE njb-55 35 67 the the DET njb-55 35 68     SPACE njb-55 35 69 repository repository NOUN njb-55 35 70     SPACE njb-55 35 71 of of ADP njb-55 35 72     SPACE njb-55 35 73 pd_admas(project pd_admas(project PUNCT njb-55 35 74     SPACE njb-55 35 75 directorate directorate NOUN njb-55 35 76     SPACE njb-55 35 77 on on ADP njb-55 35 78     SPACE njb-55 35 79 animal animal NOUN njb-55 35 80     SPACE njb-55 35 81 disease disease NOUN njb-55 35 82     SPACE njb-55 35 83 monitoring monitoring NOUN njb-55 35 84     SPACE njb-55 35 85 and and CCONJ njb-55 35 86     SPACE njb-55 35 87 surveillance surveillance NOUN njb-55 35 88 ) ) PUNCT njb-55 35 89 , , PUNCT njb-55 35 90     SPACE njb-55 35 91 uas uas NOUN njb-55 35 92 , , PUNCT njb-55 35 93     SPACE njb-55 35 94 bangalore bangalore PROPN njb-55 35 95 , , PUNCT njb-55 35 96     SPACE njb-55 35 97 india india PROPN njb-55 35 98 . . PUNCT njb-55 35 99       SPACE njb-55 36 1 cultivation cultivation NOUN njb-55 36 2     SPACE njb-55 36 3 of of ADP njb-55 36 4     SPACE njb-55 36 5 leptospira leptospira PROPN njb-55 36 6 . . PUNCT njb-55 36 7     SPACE njb-55 37 1 leptospira leptospira PROPN njb-55 37 2     SPACE njb-55 37 3 serovars serovar NOUN njb-55 37 4     SPACE njb-55 37 5 mentioned mention VERB njb-55 37 6     SPACE njb-55 37 7 above above ADP njb-55 37 8     SPACE njb-55 37 9 were be AUX njb-55 37 10     SPACE njb-55 37 11 grown grow VERB njb-55 37 12     SPACE njb-55 37 13 in in ADP njb-55 37 14     SPACE njb-55 37 15 luria‐bertani luria‐bertani NOUN njb-55 37 16     SPACE njb-55 37 17 ( ( PUNCT njb-55 37 18 lb lb X njb-55 37 19 ) ) PUNCT njb-55 37 20     SPACE njb-55 37 21 agar agar NOUN njb-55 37 22     SPACE njb-55 37 23 ( ( PUNCT njb-55 37 24 difco difco PROPN njb-55 37 25     SPACE njb-55 37 26 detroit detroit PROPN njb-55 37 27 , , PUNCT njb-55 37 28     SPACE njb-55 37 29 mi mi PROPN njb-55 37 30 , , PUNCT njb-55 37 31     SPACE njb-55 37 32 usa usa PROPN njb-55 37 33 ) ) PUNCT njb-55 37 34     SPACE njb-55 37 35 supplemented supplement VERB njb-55 37 36     SPACE njb-55 37 37 with with ADP njb-55 37 38     SPACE njb-55 37 39 1‐3 1‐3 NUM njb-55 37 40 % % NOUN njb-55 37 41     SPACE njb-55 37 42 nacl nacl PROPN njb-55 37 43     SPACE njb-55 37 44 at at ADP njb-55 37 45     SPACE njb-55 37 46 370c 370c NOUN njb-55 37 47     SPACE njb-55 37 48 overnight overnight ADV njb-55 37 49 . . PUNCT njb-55 37 50     SPACE njb-55 38 1 dna dna PROPN njb-55 38 2     SPACE njb-55 38 3 preparation preparation NOUN njb-55 38 4 . . PUNCT njb-55 38 5     SPACE njb-55 39 1 template template NOUN njb-55 39 2     SPACE njb-55 39 3 genomic genomic ADJ njb-55 39 4     SPACE njb-55 39 5 dna dna PROPN njb-55 39 6     SPACE njb-55 39 7 was be AUX njb-55 39 8     SPACE njb-55 39 9 extracted extract VERB njb-55 39 10 / / SYM njb-55 39 11 isolated isolate VERB njb-55 39 12     SPACE njb-55 39 13 from from ADP njb-55 39 14     SPACE njb-55 39 15 pure pure ADJ njb-55 39 16     SPACE njb-55 39 17 culture culture NOUN njb-55 39 18     SPACE njb-55 39 19 of of ADP njb-55 39 20     SPACE njb-55 39 21 corresponding correspond VERB njb-55 39 22     SPACE njb-55 39 23 leptospires leptospire NOUN njb-55 39 24 , , PUNCT njb-55 39 25     SPACE njb-55 39 26 using use VERB njb-55 39 27     SPACE njb-55 39 28 qiaamp qiaamp ADJ njb-55 39 29     SPACE njb-55 39 30 dna dna PROPN njb-55 39 31     SPACE njb-55 39 32 minikit minikit NOUN njb-55 39 33     SPACE njb-55 39 34 ( ( PUNCT njb-55 39 35 qiagen qiagen PROPN njb-55 39 36 , , PUNCT njb-55 39 37     SPACE njb-55 39 38 germany germany PROPN njb-55 39 39 ) ) PUNCT njb-55 39 40     SPACE njb-55 39 41 and and CCONJ njb-55 39 42     SPACE njb-55 39 43 other other ADJ njb-55 39 44     SPACE njb-55 39 45 alternative alternative ADJ njb-55 39 46     SPACE njb-55 39 47 method method NOUN njb-55 39 48     SPACE njb-55 39 49 described describe VERB njb-55 39 50     SPACE njb-55 39 51 by by ADP njb-55 39 52           SPACE njb-55 39 53 hoshino hoshino NOUN njb-55 39 54     SPACE njb-55 39 55 et et PROPN njb-55 39 56     SPACE njb-55 39 57 al al PROPN njb-55 39 58 . . PROPN njb-55 39 59 , , PUNCT njb-55 39 60     SPACE njb-55 39 61 1998 1998 NUM njb-55 39 62 . . PUNCT njb-55 39 63     SPACE njb-55 40 1 for for ADP njb-55 40 2     SPACE njb-55 40 3 it it PRON njb-55 40 4         SPACE njb-55 40 5 freshly freshly ADV njb-55 40 6     SPACE njb-55 40 7 grown grow VERB njb-55 40 8     SPACE njb-55 40 9 bacterial bacterial ADJ njb-55 40 10     SPACE njb-55 40 11 method method NOUN njb-55 40 12     SPACE njb-55 40 13 for for ADP njb-55 40 14     SPACE njb-55 40 15 diagnosis diagnosis NOUN njb-55 40 16     SPACE njb-55 40 17 of of ADP njb-55 40 18     SPACE njb-55 40 19 leptospirosis leptospirosis NOUN njb-55 40 20 , , PUNCT njb-55 40 21     SPACE njb-55 40 22 has have AUX njb-55 40 23     SPACE njb-55 40 24 been be AUX njb-55 40 25     SPACE njb-55 40 26 so so ADV njb-55 40 27     SPACE njb-55 40 28 far far ADV njb-55 40 29     SPACE njb-55 40 30 by by ADP njb-55 40 31     SPACE njb-55 40 32 culture culture NOUN njb-55 40 33     SPACE njb-55 40 34 isolation isolation NOUN njb-55 40 35     SPACE njb-55 40 36 but but CCONJ njb-55 40 37     SPACE njb-55 40 38 it it PRON njb-55 40 39     SPACE njb-55 40 40 is be AUX njb-55 40 41     SPACE njb-55 40 42 time time NOUN njb-55 40 43     SPACE njb-55 40 44 consuming consuming NOUN njb-55 40 45     SPACE njb-55 40 46 and and CCONJ njb-55 40 47     SPACE njb-55 40 48 potentially potentially ADV njb-55 40 49     SPACE njb-55 40 50 hazardous hazardous ADJ njb-55 40 51 . . PUNCT njb-55 40 52         SPACE njb-55 40 53 another another DET njb-55 40 54     SPACE njb-55 40 55 traditional traditional ADJ njb-55 40 56     SPACE njb-55 40 57 method method NOUN njb-55 40 58     SPACE njb-55 40 59 is be AUX njb-55 40 60     SPACE njb-55 40 61 the the DET njb-55 40 62     SPACE njb-55 40 63 detection detection NOUN njb-55 40 64     SPACE njb-55 40 65 of of ADP njb-55 40 66     SPACE njb-55 40 67 antibodies antibody NOUN njb-55 40 68     SPACE njb-55 40 69 ( ( PUNCT njb-55 40 70 serological serological ADJ njb-55 40 71     SPACE njb-55 40 72 tests test NOUN njb-55 40 73 ) ) PUNCT njb-55 40 74     SPACE njb-55 40 75 which which PRON njb-55 40 76     SPACE njb-55 40 77 is be AUX njb-55 40 78     SPACE njb-55 40 79 also also ADV njb-55 40 80     SPACE njb-55 40 81 a a DET njb-55 40 82     SPACE njb-55 40 83 time time NOUN njb-55 40 84     SPACE njb-55 40 85 consuming consuming NOUN njb-55 40 86     SPACE njb-55 40 87 method method NOUN njb-55 40 88     SPACE njb-55 40 89 and and CCONJ njb-55 40 90     SPACE njb-55 40 91 fails fail VERB njb-55 40 92     SPACE njb-55 40 93 to to PART njb-55 40 94     SPACE njb-55 40 95 identify identify VERB njb-55 40 96     SPACE njb-55 40 97 the the DET njb-55 40 98     SPACE njb-55 40 99 infecting infect VERB njb-55 40 100     SPACE njb-55 40 101 serovar serovar NOUN njb-55 40 102 . . PUNCT njb-55 40 103         SPACE njb-55 41 1 classification classification NOUN njb-55 41 2     SPACE njb-55 41 3 and and CCONJ njb-55 41 4     SPACE njb-55 41 5 identification identification NOUN njb-55 41 6     SPACE njb-55 41 7 of of ADP njb-55 41 8     SPACE njb-55 41 9 leptospires leptospire NOUN njb-55 41 10     SPACE njb-55 41 11 using use VERB njb-55 41 12     SPACE njb-55 41 13 serology serology NOUN njb-55 41 14     SPACE njb-55 41 15 is be AUX njb-55 41 16     SPACE njb-55 41 17 a a DET njb-55 41 18     SPACE njb-55 41 19 difficult difficult ADJ njb-55 41 20     SPACE njb-55 41 21 and and CCONJ njb-55 41 22     SPACE njb-55 41 23 tedious tedious ADJ njb-55 41 24     SPACE njb-55 41 25 procedure procedure NOUN njb-55 41 26 . . PUNCT njb-55 41 27         SPACE njb-55 42 1 recently recently ADV njb-55 42 2     SPACE njb-55 42 3 molecular molecular ADJ njb-55 42 4     SPACE njb-55 42 5 biology biology NOUN njb-55 42 6     SPACE njb-55 42 7 techniques technique NOUN njb-55 42 8     SPACE njb-55 42 9 have have AUX njb-55 42 10     SPACE njb-55 42 11 been be AUX njb-55 42 12     SPACE njb-55 42 13 introduced introduce VERB njb-55 42 14     SPACE njb-55 42 15 for for ADP njb-55 42 16     SPACE njb-55 42 17 detection detection NOUN njb-55 42 18 . . PUNCT njb-55 42 19       SPACE njb-55 42 20 these these DET njb-55 42 21     SPACE njb-55 42 22 techniques technique NOUN njb-55 42 23     SPACE njb-55 42 24 are be AUX njb-55 42 25     SPACE njb-55 42 26 predominantly predominantly ADV njb-55 42 27     SPACE njb-55 42 28 used use VERB njb-55 42 29     SPACE njb-55 42 30 for for ADP njb-55 42 31     SPACE njb-55 42 32 facilitating facilitate VERB njb-55 42 33     SPACE njb-55 42 34 the the DET njb-55 42 35     SPACE njb-55 42 36 study study NOUN njb-55 42 37     SPACE njb-55 42 38 of of ADP njb-55 42 39     SPACE njb-55 42 40 the the DET njb-55 42 41     SPACE njb-55 42 42 epidemiology epidemiology NOUN njb-55 42 43     SPACE njb-55 42 44 of of ADP njb-55 42 45     SPACE njb-55 42 46 leptospirosis leptospirosis NOUN njb-55 42 47 . . PUNCT njb-55 42 48         SPACE njb-55 42 49 genetic genetic ADJ njb-55 42 50     SPACE njb-55 42 51 taxonomy taxonomy NOUN njb-55 42 52     SPACE njb-55 42 53 involves involve VERB njb-55 42 54     SPACE njb-55 42 55 dna dna PROPN njb-55 42 56 / / SYM njb-55 42 57 dna dna PROPN njb-55 42 58     SPACE njb-55 42 59 hybridisation hybridisation NOUN njb-55 42 60     SPACE njb-55 42 61 and and CCONJ njb-55 42 62     SPACE njb-55 42 63 gc gc PROPN njb-55 42 64     SPACE njb-55 42 65 content content NOUN njb-55 42 66     SPACE njb-55 42 67 the the DET njb-55 42 68     SPACE njb-55 42 69 guanine‐plus‐cytosine guanine‐plus‐cytosine NOUN njb-55 42 70     SPACE njb-55 42 71 mol mol PROPN njb-55 42 72     SPACE njb-55 42 73 percentages percentage NOUN njb-55 42 74     SPACE njb-55 42 75 ( ( PUNCT njb-55 42 76 g+c g+c PROPN njb-55 42 77     SPACE njb-55 42 78 mol mol PROPN njb-55 42 79     SPACE njb-55 42 80 % % NOUN njb-55 42 81 ) ) PUNCT njb-55 42 82     SPACE njb-55 42 83 content content NOUN njb-55 42 84     SPACE njb-55 42 85 of of ADP njb-55 42 86     SPACE njb-55 42 87 dna dna NOUN njb-55 42 88 . . PUNCT njb-55 42 89     SPACE njb-55 42 90 the the DET njb-55 42 91     SPACE njb-55 42 92 genotypic genotypic NOUN njb-55 42 93     SPACE njb-55 42 94 classification classification NOUN njb-55 42 95     SPACE njb-55 42 96 based base VERB njb-55 42 97     SPACE njb-55 42 98 on on ADP njb-55 42 99     SPACE njb-55 42 100 dna dna NOUN njb-55 42 101     SPACE njb-55 42 102 hybridization hybridization NOUN njb-55 42 103     SPACE njb-55 42 104 defined define VERB njb-55 42 105     SPACE njb-55 42 106 21 21 NUM njb-55 42 107     SPACE njb-55 42 108 genome genome NOUN njb-55 42 109     SPACE njb-55 42 110 species specie NOUN njb-55 42 111     SPACE njb-55 42 112 of of ADP njb-55 42 113     SPACE njb-55 42 114 leptospira leptospira PROPN njb-55 42 115     SPACE njb-55 42 116 that that PRON njb-55 42 117     SPACE njb-55 42 118 include include VERB njb-55 42 119     SPACE njb-55 42 120 29 29 NUM njb-55 42 121     SPACE njb-55 42 122 serogroups serogroup NOUN njb-55 42 123     SPACE njb-55 42 124 and and CCONJ njb-55 42 125     SPACE njb-55 42 126 269 269 NUM njb-55 42 127     SPACE njb-55 42 128 serovars serovar NOUN njb-55 42 129 . . PUNCT njb-55 42 130     SPACE njb-55 43 1 yasuda yasuda PROPN njb-55 43 2     SPACE njb-55 43 3 et et PROPN njb-55 43 4     SPACE njb-55 43 5 al al PROPN njb-55 43 6 . . PROPN njb-55 43 7 , , PUNCT njb-55 43 8     SPACE njb-55 43 9 1987 1987 NUM njb-55 43 10     SPACE njb-55 43 11 proposed propose VERB njb-55 43 12     SPACE njb-55 43 13 a a DET njb-55 43 14     SPACE njb-55 43 15 new new ADJ njb-55 43 16     SPACE njb-55 43 17 classification classification NOUN njb-55 43 18     SPACE njb-55 43 19 of of ADP njb-55 43 20     SPACE njb-55 43 21 leptospira leptospira PROPN njb-55 43 22     SPACE njb-55 43 23 based base VERB njb-55 43 24     SPACE njb-55 43 25 on on ADP njb-55 43 26         SPACE njb-55 43 27 dna dna PROPN njb-55 43 28     SPACE njb-55 43 29 homology homology NOUN njb-55 43 30     SPACE njb-55 43 31 study study NOUN njb-55 43 32     SPACE njb-55 43 33 on on ADP njb-55 43 34     SPACE njb-55 43 35 46 46 NUM njb-55 43 36     SPACE njb-55 43 37 pathogenic pathogenic ADJ njb-55 43 38     SPACE njb-55 43 39 and and CCONJ njb-55 43 40     SPACE njb-55 43 41 non non ADJ njb-55 43 42     SPACE njb-55 43 43 pathogenic pathogenic ADJ njb-55 43 44     SPACE njb-55 43 45 serovars serovar NOUN njb-55 43 46 . . PUNCT njb-55 43 47     SPACE njb-55 44 1 woodward woodward NOUN njb-55 44 2     SPACE njb-55 44 3 and and CCONJ njb-55 44 4     SPACE njb-55 44 5 redstone redstone NOUN njb-55 44 6 , , PUNCT njb-55 44 7     SPACE njb-55 44 8 1994 1994 NUM njb-55 44 9     SPACE njb-55 44 10 developed develop VERB njb-55 44 11     SPACE njb-55 44 12 a a DET njb-55 44 13     SPACE njb-55 44 14 polymerase polymerase NOUN njb-55 44 15     SPACE njb-55 44 16 chain chain NOUN njb-55 44 17     SPACE njb-55 44 18 reaction reaction NOUN njb-55 44 19     SPACE njb-55 44 20 ( ( PUNCT njb-55 44 21 pcr pcr PROPN njb-55 44 22 ) ) PUNCT njb-55 44 23     SPACE njb-55 44 24 combined combine VERB njb-55 44 25     SPACE njb-55 44 26 with with ADP njb-55 44 27     SPACE njb-55 44 28 restriction restriction NOUN njb-55 44 29     SPACE njb-55 44 30 fragment fragment ADJ njb-55 44 31     SPACE njb-55 44 32 length length NOUN njb-55 44 33     SPACE njb-55 44 34 polymorphism polymorphism NOUN njb-55 44 35     SPACE njb-55 44 36 which which PRON njb-55 44 37     SPACE njb-55 44 38 can can AUX njb-55 44 39     SPACE njb-55 44 40 be be AUX njb-55 44 41     SPACE njb-55 44 42 used use VERB njb-55 44 43     SPACE njb-55 44 44 for for ADP njb-55 44 45     SPACE njb-55 44 46 differentiation differentiation NOUN njb-55 44 47     SPACE njb-55 44 48 of of ADP njb-55 44 49     SPACE njb-55 44 50 leptospira leptospira PROPN njb-55 44 51     SPACE njb-55 44 52 serovars serovar NOUN njb-55 44 53 . . PUNCT njb-55 44 54       SPACE njb-55 45 1 it it PRON njb-55 45 2     SPACE njb-55 45 3 is be AUX njb-55 45 4     SPACE njb-55 45 5 suggested suggest VERB njb-55 45 6     SPACE njb-55 45 7 that that SCONJ njb-55 45 8     SPACE njb-55 45 9 the the DET njb-55 45 10     SPACE njb-55 45 11 pcr pcr NOUN njb-55 45 12     SPACE njb-55 45 13 combined combine VERB njb-55 45 14     SPACE njb-55 45 15 with with ADP njb-55 45 16     SPACE njb-55 45 17 restriction restriction NOUN njb-55 45 18     SPACE njb-55 45 19 fragment fragment ADJ njb-55 45 20     SPACE njb-55 45 21 length length NOUN njb-55 45 22     SPACE njb-55 45 23 polymorphism polymorphism NOUN njb-55 45 24     SPACE njb-55 45 25 is be AUX njb-55 45 26     SPACE njb-55 45 27 useful useful ADJ njb-55 45 28     SPACE njb-55 45 29 tool tool NOUN njb-55 45 30     SPACE njb-55 45 31 for for ADP njb-55 45 32     SPACE njb-55 45 33 rapid rapid ADJ njb-55 45 34     SPACE njb-55 45 35 detection detection NOUN njb-55 45 36     SPACE njb-55 45 37 and and CCONJ njb-55 45 38     SPACE njb-55 45 39 preliminary preliminary ADJ njb-55 45 40     SPACE njb-55 45 41 differentiation differentiation NOUN njb-55 45 42     SPACE njb-55 45 43 of of ADP njb-55 45 44     SPACE njb-55 45 45 leptospires leptospire NOUN njb-55 45 46     SPACE njb-55 45 47 ( ( PUNCT njb-55 45 48 levett levett PROPN njb-55 45 49     SPACE njb-55 45 50 et et PROPN njb-55 45 51     SPACE njb-55 45 52 al al PROPN njb-55 45 53 . . PROPN njb-55 45 54 , , PUNCT njb-55 45 55     SPACE njb-55 45 56 2001 2001 NUM njb-55 45 57 ) ) PUNCT njb-55 45 58 . . PUNCT njb-55 45 59     SPACE njb-55 46 1 although although SCONJ njb-55 46 2     SPACE njb-55 46 3 over over ADP njb-55 46 4     SPACE njb-55 46 5 269 269 NUM njb-55 46 6     SPACE njb-55 46 7 serovars serovar NOUN njb-55 46 8     SPACE njb-55 46 9 of of ADP njb-55 46 10     SPACE njb-55 46 11 leptospira leptospira PROPN njb-55 46 12     SPACE njb-55 46 13 have have AUX njb-55 46 14     SPACE njb-55 46 15 been be AUX njb-55 46 16     SPACE njb-55 46 17 described describe VERB njb-55 46 18 , , PUNCT njb-55 46 19     SPACE njb-55 46 20 all all DET njb-55 46 21     SPACE njb-55 46 22 members member NOUN njb-55 46 23     SPACE njb-55 46 24 of of ADP njb-55 46 25     SPACE njb-55 46 26 the the DET njb-55 46 27     SPACE njb-55 46 28 genus genus NOUN njb-55 46 29     SPACE njb-55 46 30 have have VERB njb-55 46 31     SPACE njb-55 46 32 similar similar ADJ njb-55 46 33     SPACE njb-55 46 34 morphology morphology NOUN njb-55 46 35 . . PUNCT njb-55 46 36     SPACE njb-55 47 1 leptospira leptospira PROPN njb-55 47 2     SPACE njb-55 47 3 are be AUX njb-55 47 4     SPACE njb-55 47 5 spiral‐shaped spiral‐shaped AUX njb-55 47 6     SPACE njb-55 47 7 bacteria bacteria NOUN njb-55 47 8     SPACE njb-55 47 9 that that PRON njb-55 47 10     SPACE njb-55 47 11 are be AUX njb-55 47 12     SPACE njb-55 47 13 6‐20 6‐20 NUM njb-55 47 14     SPACE njb-55 47 15 μm μm NOUN njb-55 47 16     SPACE njb-55 47 17 long long ADJ njb-55 47 18     SPACE njb-55 47 19 and and CCONJ njb-55 47 20     SPACE njb-55 47 21 0.1 0.1 NUM njb-55 47 22     SPACE njb-55 47 23 μm μm NOUN njb-55 47 24     SPACE njb-55 47 25 in in ADP njb-55 47 26     SPACE njb-55 47 27 diameter diameter NOUN njb-55 47 28 . . PUNCT njb-55 47 29       SPACE njb-55 47 30 one one NOUN njb-55 47 31     SPACE njb-55 47 32 or or CCONJ njb-55 47 33     SPACE njb-55 47 34 both both PRON njb-55 47 35     SPACE njb-55 47 36 ends end VERB njb-55 47 37     SPACE njb-55 47 38 of of ADP njb-55 47 39     SPACE njb-55 47 40 the the DET njb-55 47 41     SPACE njb-55 47 42 spirochete spirochete NOUN njb-55 47 43     SPACE njb-55 47 44 are be AUX njb-55 47 45     SPACE njb-55 47 46 usually usually ADV njb-55 47 47     SPACE njb-55 47 48 hooked hooked ADJ njb-55 47 49 . . PUNCT njb-55 47 50     SPACE njb-55 47 51 because because SCONJ njb-55 47 52     SPACE njb-55 47 53 they they PRON njb-55 47 54     SPACE njb-55 47 55 are be AUX njb-55 47 56     SPACE njb-55 47 57 so so ADV njb-55 47 58     SPACE njb-55 47 59 thin thin ADJ njb-55 47 60 , , PUNCT njb-55 47 61     SPACE njb-55 47 62 live live VERB njb-55 47 63     SPACE njb-55 47 64 leptospira leptospira PROPN njb-55 47 65     SPACE njb-55 47 66 are be AUX njb-55 47 67     SPACE njb-55 47 68 best well ADV njb-55 47 69     SPACE njb-55 47 70 observed observe VERB njb-55 47 71     SPACE njb-55 47 72 by by ADP njb-55 47 73     SPACE njb-55 47 74 dark dark ADJ njb-55 47 75     SPACE njb-55 47 76 field field NOUN njb-55 47 77     SPACE njb-55 47 78 microscopy microscopy NOUN njb-55 47 79 . . PUNCT njb-55 47 80     SPACE njb-55 48 1 the the DET njb-55 48 2     SPACE njb-55 48 3 bacteria bacteria NOUN njb-55 48 4     SPACE njb-55 48 5 have have VERB njb-55 48 6     SPACE njb-55 48 7 a a DET njb-55 48 8     SPACE njb-55 48 9 number number NOUN njb-55 48 10     SPACE njb-55 48 11 of of ADP njb-55 48 12     SPACE njb-55 48 13 freedom freedom NOUN njb-55 48 14     SPACE njb-55 48 15 degrees degree NOUN njb-55 48 16 ; ; PUNCT njb-55 48 17     SPACE njb-55 48 18 when when SCONJ njb-55 48 19     SPACE njb-55 48 20 ready ready ADJ njb-55 48 21     SPACE njb-55 48 22 to to PART njb-55 48 23     SPACE njb-55 48 24 proliferate proliferate VERB njb-55 48 25     SPACE njb-55 48 26 via via ADP njb-55 48 27     SPACE njb-55 48 28 binary binary ADJ njb-55 48 29     SPACE njb-55 48 30 fission fission NOUN njb-55 48 31 , , PUNCT njb-55 48 32     SPACE njb-55 48 33 the the DET njb-55 48 34     SPACE njb-55 48 35 bacterium bacterium NOUN njb-55 48 36     SPACE njb-55 48 37 noticeably noticeably ADV njb-55 48 38     SPACE njb-55 48 39 bends bend NOUN njb-55 48 40     SPACE njb-55 48 41 in in ADP njb-55 48 42     SPACE njb-55 48 43 the the DET njb-55 48 44     SPACE njb-55 48 45 place place NOUN njb-55 48 46     SPACE njb-55 48 47 of of ADP njb-55 48 48     SPACE njb-55 48 49 the the DET njb-55 48 50     SPACE njb-55 48 51 future future NOUN njb-55 48 52     SPACE njb-55 48 53 split split NOUN njb-55 48 54     SPACE njb-55 48 55 ( ( PUNCT njb-55 48 56 levett levett PROPN njb-55 48 57     SPACE njb-55 48 58 et et PROPN njb-55 48 59     SPACE njb-55 48 60 al al PROPN njb-55 48 61 . . PROPN njb-55 48 62 , , PUNCT njb-55 48 63     SPACE njb-55 48 64 2005 2005 NUM njb-55 48 65 ) ) PUNCT njb-55 48 66 . . PUNCT njb-55 48 67     SPACE njb-55 49 1 leptospira leptospira PROPN njb-55 49 2     SPACE njb-55 49 3 have have VERB njb-55 49 4     SPACE njb-55 49 5 a a DET njb-55 49 6     SPACE njb-55 49 7 gram‐negative‐like gram‐negative‐like NOUN njb-55 49 8     SPACE njb-55 49 9 cell cell NOUN njb-55 49 10     SPACE njb-55 49 11 envelope envelope NOUN njb-55 49 12     SPACE njb-55 49 13 consisting consist VERB njb-55 49 14     SPACE njb-55 49 15 of of ADP njb-55 49 16     SPACE njb-55 49 17 a a DET njb-55 49 18     SPACE njb-55 49 19 cytoplasmic cytoplasmic ADJ njb-55 49 20     SPACE njb-55 49 21 and and CCONJ njb-55 49 22     SPACE njb-55 49 23 outer outer ADJ njb-55 49 24     SPACE njb-55 49 25 membrane membrane NOUN njb-55 49 26 . . PUNCT njb-55 49 27     SPACE njb-55 49 28 however however ADV njb-55 49 29 , , PUNCT njb-55 49 30     SPACE njb-55 49 31 the the DET njb-55 49 32     SPACE njb-55 49 33 peptidoglycan peptidoglycan NOUN njb-55 49 34     SPACE njb-55 49 35 layer layer NOUN njb-55 49 36     SPACE njb-55 49 37 is be AUX njb-55 49 38     SPACE njb-55 49 39 associated associate VERB njb-55 49 40     SPACE njb-55 49 41 with with ADP njb-55 49 42     SPACE njb-55 49 43 the the DET njb-55 49 44     SPACE njb-55 49 45 cytoplasmic cytoplasmic ADJ njb-55 49 46     SPACE njb-55 49 47 rather rather ADV njb-55 49 48     SPACE njb-55 49 49 than than ADP njb-55 49 50     SPACE njb-55 49 51 the the DET njb-55 49 52     SPACE njb-55 49 53 outer outer ADJ njb-55 49 54     SPACE njb-55 49 55 membrane membrane NOUN njb-55 49 56 , , PUNCT njb-55 49 57     SPACE njb-55 49 58 an an DET njb-55 49 59     SPACE njb-55 49 60 arrangement arrangement NOUN njb-55 49 61     SPACE njb-55 49 62 that that PRON njb-55 49 63     SPACE njb-55 49 64 is be AUX njb-55 49 65     SPACE njb-55 49 66 unique unique ADJ njb-55 49 67     SPACE njb-55 49 68 to to ADP njb-55 49 69     SPACE njb-55 49 70 spirochetes spirochete NOUN njb-55 49 71 . . PUNCT njb-55 49 72     SPACE njb-55 50 1 the the DET njb-55 50 2     SPACE njb-55 50 3 two two NUM njb-55 50 4     SPACE njb-55 50 5 flagella flagella NOUN njb-55 50 6     SPACE njb-55 50 7 of of ADP njb-55 50 8     SPACE njb-55 50 9 leptospira leptospira PROPN njb-55 50 10     SPACE njb-55 50 11 extend extend VERB njb-55 50 12     SPACE njb-55 50 13 from from ADP njb-55 50 14     SPACE njb-55 50 15 the the DET njb-55 50 16     SPACE njb-55 50 17 cytoplasmic cytoplasmic ADJ njb-55 50 18     SPACE njb-55 50 19 membrane membrane NOUN njb-55 50 20     SPACE njb-55 50 21 at at ADP njb-55 50 22     SPACE njb-55 50 23 the the DET njb-55 50 24     SPACE njb-55 50 25 ends end NOUN njb-55 50 26     SPACE njb-55 50 27 of of ADP njb-55 50 28     SPACE njb-55 50 29 the the DET njb-55 50 30     SPACE njb-55 50 31 bacteria bacteria NOUN njb-55 50 32     SPACE njb-55 50 33 into into ADP njb-55 50 34     SPACE njb-55 50 35 the the DET njb-55 50 36     SPACE njb-55 50 37 periplasmic periplasmic ADJ njb-55 50 38     SPACE njb-55 50 39 space space NOUN njb-55 50 40     SPACE njb-55 50 41 and and CCONJ njb-55 50 42     SPACE njb-55 50 43 are be AUX njb-55 50 44     SPACE njb-55 50 45 necessary necessary ADJ njb-55 50 46     SPACE njb-55 50 47 for for ADP njb-55 50 48     SPACE njb-55 50 49 the the DET njb-55 50 50     SPACE njb-55 50 51 motility motility NOUN njb-55 50 52     SPACE njb-55 50 53 of of ADP njb-55 50 54     SPACE njb-55 50 55 leptospira leptospira PROPN njb-55 50 56 . . PUNCT njb-55 50 57     SPACE njb-55 51 1 the the DET njb-55 51 2     SPACE njb-55 51 3 outer outer ADJ njb-55 51 4     SPACE njb-55 51 5 membrane membrane NOUN njb-55 51 6     SPACE njb-55 51 7 contains contain VERB njb-55 51 8     SPACE njb-55 51 9 a a DET njb-55 51 10     SPACE njb-55 51 11 variety variety NOUN njb-55 51 12     SPACE njb-55 51 13 of of ADP njb-55 51 14     SPACE njb-55 51 15 lipoproteins lipoprotein NOUN njb-55 51 16     SPACE njb-55 51 17 and and CCONJ njb-55 51 18     SPACE njb-55 51 19 transmembrane transmembrane NOUN njb-55 51 20     SPACE njb-55 51 21 outer outer ADJ njb-55 51 22     SPACE njb-55 51 23 membrane membrane NOUN njb-55 51 24     SPACE njb-55 51 25 proteins protein NOUN njb-55 51 26 . . PUNCT njb-55 51 27     SPACE njb-55 51 28 as as ADV njb-55 51 29     SPACE njb-55 51 30 expected expect VERB njb-55 51 31 , , PUNCT njb-55 51 32     SPACE njb-55 51 33 the the DET njb-55 51 34     SPACE njb-55 51 35 protein protein NOUN njb-55 51 36     SPACE njb-55 51 37 composition composition NOUN njb-55 51 38     SPACE njb-55 51 39 of of ADP njb-55 51 40     SPACE njb-55 51 41 the the DET njb-55 51 42     SPACE njb-55 51 43 outer outer ADJ njb-55 51 44     SPACE njb-55 51 45 membrane membrane NOUN njb-55 51 46     SPACE njb-55 51 47 differs differ VERB njb-55 51 48     SPACE njb-55 51 49 when when SCONJ njb-55 51 50     SPACE njb-55 51 51 comparing compare VERB njb-55 51 52     SPACE njb-55 51 53 leptospira leptospira PROPN njb-55 51 54     SPACE njb-55 51 55 growing grow VERB njb-55 51 56     SPACE njb-55 51 57 nepal nepal ADJ njb-55 51 58     SPACE njb-55 51 59 journal journal NOUN njb-55 51 60     SPACE njb-55 51 61 of of ADP njb-55 51 62     SPACE njb-55 51 63 biotechnology biotechnology NOUN njb-55 51 64 . . PUNCT njb-55 51 65     SPACE njb-55 52 1 jan jan PROPN njb-55 52 2 . . PROPN njb-55 52 3     SPACE njb-55 52 4 2011 2011 NUM njb-55 52 5 , , PUNCT njb-55 52 6     SPACE njb-55 52 7 vol vol NOUN njb-55 52 8 . . PUNCT njb-55 52 9     SPACE njb-55 52 10 1 1 NUM njb-55 52 11 , , PUNCT njb-55 52 12     SPACE njb-55 52 13 no no INTJ njb-55 52 14 . . PUNCT njb-55 52 15     SPACE njb-55 52 16 1 1 NUM njb-55 52 17 : : PUNCT njb-55 52 18     SPACE njb-55 52 19 22‐30 22‐30 NUM njb-55 52 20     SPACE njb-55 52 21 24 24 NUM njb-55 52 22     SPACE njb-55 52 23 biotechnology biotechnology NOUN njb-55 52 24     SPACE njb-55 52 25 society society NOUN njb-55 52 26     SPACE njb-55 52 27 of of ADP njb-55 52 28     SPACE njb-55 52 29 nepal nepal NOUN njb-55 52 30     SPACE njb-55 52 31 ( ( PUNCT njb-55 52 32 bsn bsn PROPN njb-55 52 33 ) ) PUNCT njb-55 52 34 , , PUNCT njb-55 52 35     SPACE njb-55 52 36 all all DET njb-55 52 37     SPACE njb-55 52 38 rights right NOUN njb-55 52 39     SPACE njb-55 52 40 reserved reserve VERB njb-55 52 41       SPACE njb-55 52 42 step step NOUN njb-55 52 43     SPACE njb-55 52 44 at at ADP njb-55 52 45     SPACE njb-55 52 46 720c 720c NOUN njb-55 52 47     SPACE njb-55 52 48 for for ADP njb-55 52 49     SPACE njb-55 52 50 1 1 NUM njb-55 52 51     SPACE njb-55 52 52 min min NOUN njb-55 52 53     SPACE njb-55 52 54 and and CCONJ njb-55 52 55     SPACE njb-55 52 56 a a DET njb-55 52 57     SPACE njb-55 52 58 final final ADJ njb-55 52 59     SPACE njb-55 52 60 elongation elongation NOUN njb-55 52 61     SPACE njb-55 52 62 at at ADP njb-55 52 63     SPACE njb-55 52 64 720c 720c NOUN njb-55 52 65     SPACE njb-55 52 66 for for ADP njb-55 52 67     SPACE njb-55 52 68 5 5 NUM njb-55 52 69     SPACE njb-55 52 70 min min PROPN njb-55 52 71 . . PUNCT njb-55 52 72     SPACE njb-55 52 73 finally finally ADV njb-55 52 74     SPACE njb-55 52 75 annealing anneal VERB njb-55 52 76     SPACE njb-55 52 77 temperature temperature NOUN njb-55 52 78     SPACE njb-55 52 79 was be AUX njb-55 52 80     SPACE njb-55 52 81 standardized standardize VERB njb-55 52 82     SPACE njb-55 52 83 to to ADP njb-55 52 84     SPACE njb-55 52 85 600c 600c DET njb-55 52 86     SPACE njb-55 52 87 for for ADP njb-55 52 88     SPACE njb-55 52 89 1 1 NUM njb-55 52 90     SPACE njb-55 52 91 min min NOUN njb-55 52 92     SPACE njb-55 52 93 ( ( PUNCT njb-55 52 94 fig fig NOUN njb-55 52 95 . . PUNCT njb-55 52 96     SPACE njb-55 52 97 1a 1a NUM njb-55 52 98 ) ) PUNCT njb-55 52 99 . . PUNCT njb-55 52 100         SPACE njb-55 53 1 the the DET njb-55 53 2     SPACE njb-55 53 3 pcr pcr NOUN njb-55 53 4     SPACE njb-55 53 5 standardization standardization NOUN njb-55 53 6     SPACE njb-55 53 7 using use VERB njb-55 53 8     SPACE njb-55 53 9 primers primer NOUN njb-55 53 10     SPACE njb-55 53 11 p30/31 p30/31 PROPN njb-55 53 12     SPACE njb-55 53 13 was be AUX njb-55 53 14     SPACE njb-55 53 15 initiated initiate VERB njb-55 53 16     SPACE njb-55 53 17 in in ADP njb-55 53 18     SPACE njb-55 53 19 a a DET njb-55 53 20     SPACE njb-55 53 21 25 25 NUM njb-55 53 22     SPACE njb-55 53 23 ml ml PROPN njb-55 53 24     SPACE njb-55 53 25 reaction reaction NOUN njb-55 53 26     SPACE njb-55 53 27 volume volume NOUN njb-55 53 28     SPACE njb-55 53 29 containing contain VERB njb-55 53 30     SPACE njb-55 53 31 10x 10x PROPN njb-55 53 32     SPACE njb-55 53 33 taq taq PROPN njb-55 53 34     SPACE njb-55 53 35 buffer buffer NOUN njb-55 53 36     SPACE njb-55 53 37 with with ADP njb-55 53 38     SPACE njb-55 53 39 kcl kcl PROPN njb-55 53 40     SPACE njb-55 53 41 ( ( PUNCT njb-55 53 42 100 100 NUM njb-55 53 43 mm mm PROPN njb-55 53 44     SPACE njb-55 53 45 tris tris PROPN njb-55 53 46     SPACE njb-55 53 47 hcl hcl PROPN njb-55 53 48 , , PUNCT njb-55 53 49     SPACE njb-55 53 50 50 50 NUM njb-55 53 51 mm mm NOUN njb-55 53 52     SPACE njb-55 53 53 kcl kcl PROPN njb-55 53 54 , , PUNCT njb-55 53 55     SPACE njb-55 53 56 0.8 0.8 NUM njb-55 53 57 % % NOUN njb-55 53 58 nonidet nonidet NOUN njb-55 53 59     SPACE njb-55 53 60 p40 p40 NOUN njb-55 53 61 ) ) PUNCT njb-55 53 62 , , PUNCT njb-55 53 63     SPACE njb-55 53 64 1.5 1.5 NUM njb-55 53 65 mm mm PROPN njb-55 53 66     SPACE njb-55 53 67 mgcl2 mgcl2 PROPN njb-55 53 68 , , PUNCT njb-55 53 69     SPACE njb-55 53 70 2.5 2.5 NUM njb-55 53 71 mm mm NOUN njb-55 53 72     SPACE njb-55 53 73 of of ADP njb-55 53 74     SPACE njb-55 53 75 each each DET njb-55 53 76     SPACE njb-55 53 77 dntps dntps NOUN njb-55 53 78 , , PUNCT njb-55 53 79     SPACE njb-55 53 80 2u 2u NUM njb-55 53 81     SPACE njb-55 53 82 of of ADP njb-55 53 83     SPACE njb-55 53 84 taq taq PROPN njb-55 53 85     SPACE njb-55 53 86 polymerase polymerase NOUN njb-55 53 87 , , PUNCT njb-55 53 88     SPACE njb-55 53 89 25pmoles 25pmoles NUM njb-55 53 90     SPACE njb-55 53 91 of of ADP njb-55 53 92     SPACE njb-55 53 93 each each DET njb-55 53 94     SPACE njb-55 53 95 primer primer NOUN njb-55 53 96     SPACE njb-55 53 97 and and CCONJ njb-55 53 98     SPACE njb-55 53 99 0.25ml 0.25ml NOUN njb-55 53 100     SPACE njb-55 53 101 of of ADP njb-55 53 102     SPACE njb-55 53 103 dna dna NOUN njb-55 53 104     SPACE njb-55 53 105 ( ( PUNCT njb-55 53 106 40ng 40ng NOUN njb-55 53 107 / / SYM njb-55 53 108 ml ml PROPN njb-55 53 109 ) ) PUNCT njb-55 53 110     SPACE njb-55 53 111 extract extract NOUN njb-55 53 112     SPACE njb-55 53 113 from from ADP njb-55 53 114     SPACE njb-55 53 115 l. l. PROPN njb-55 53 116     SPACE njb-55 53 117 javanica javanica PROPN njb-55 53 118 . . PUNCT njb-55 53 119     SPACE njb-55 54 1 the the DET njb-55 54 2     SPACE njb-55 54 3 test test NOUN njb-55 54 4     SPACE njb-55 54 5 was be AUX njb-55 54 6     SPACE njb-55 54 7 done do VERB njb-55 54 8     SPACE njb-55 54 9 for for ADP njb-55 54 10     SPACE njb-55 54 11 several several ADJ njb-55 54 12     SPACE njb-55 54 13 combinations combination NOUN njb-55 54 14     SPACE njb-55 54 15 annealing anneal VERB njb-55 54 16     SPACE njb-55 54 17 temperatures temperature NOUN njb-55 54 18     SPACE njb-55 54 19 from from ADP njb-55 54 20     SPACE njb-55 54 21 550c 550c NOUN njb-55 54 22     SPACE njb-55 54 23 to to ADP njb-55 54 24     SPACE njb-55 54 25 650c 650c PROPN njb-55 54 26 . . PUNCT njb-55 54 27     SPACE njb-55 55 1 the the DET njb-55 55 2     SPACE njb-55 55 3 reaction reaction NOUN njb-55 55 4     SPACE njb-55 55 5 started start VERB njb-55 55 6     SPACE njb-55 55 7 with with ADP njb-55 55 8     SPACE njb-55 55 9 an an DET njb-55 55 10     SPACE njb-55 55 11 initial initial ADJ njb-55 55 12     SPACE njb-55 55 13 denaturation denaturation NOUN njb-55 55 14     SPACE njb-55 55 15 temperature temperature NOUN njb-55 55 16     SPACE njb-55 55 17 of of ADP njb-55 55 18     SPACE njb-55 55 19 950c 950c PROPN njb-55 55 20     SPACE njb-55 55 21 for for ADP njb-55 55 22     SPACE njb-55 55 23 5 5 NUM njb-55 55 24     SPACE njb-55 55 25 min min NOUN njb-55 55 26     SPACE njb-55 55 27 followed follow VERB njb-55 55 28     SPACE njb-55 55 29 35 35 NUM njb-55 55 30     SPACE njb-55 55 31 cycles cycle NOUN njb-55 55 32     SPACE njb-55 55 33 of of ADP njb-55 55 34     SPACE njb-55 55 35 denaturing denature VERB njb-55 55 36     SPACE njb-55 55 37 at at ADP njb-55 55 38     SPACE njb-55 55 39 950c 950c PROPN njb-55 55 40     SPACE njb-55 55 41 for for ADP njb-55 55 42     SPACE njb-55 55 43 1 1 NUM njb-55 55 44     SPACE njb-55 55 45 min min PROPN njb-55 55 46 , , PUNCT njb-55 55 47     SPACE njb-55 55 48 annealing anneal VERB njb-55 55 49     SPACE njb-55 55 50 at at ADP njb-55 55 51     SPACE njb-55 55 52 600c 600c PROPN njb-55 55 53     SPACE njb-55 55 54 for for ADP njb-55 55 55     SPACE njb-55 55 56 1 1 NUM njb-55 55 57     SPACE njb-55 55 58 min min NOUN njb-55 55 59     SPACE njb-55 55 60 at at ADP njb-55 55 61     SPACE njb-55 55 62 g g NOUN njb-55 55 63     SPACE njb-55 55 64 ± ± NOUN njb-55 55 65     SPACE njb-55 55 66 50c 50c NOUN njb-55 55 67 , , PUNCT njb-55 55 68     SPACE njb-55 55 69 elongation elongation NOUN njb-55 55 70     SPACE njb-55 55 71 step step NOUN njb-55 55 72     SPACE njb-55 55 73 at at ADP njb-55 55 74     SPACE njb-55 55 75 720c 720c NOUN njb-55 55 76     SPACE njb-55 55 77 for for ADP njb-55 55 78     SPACE njb-55 55 79 45 45 NUM njb-55 55 80     SPACE njb-55 55 81 sec sec PROPN njb-55 55 82     SPACE njb-55 55 83 and and CCONJ njb-55 55 84     SPACE njb-55 55 85 a a DET njb-55 55 86     SPACE njb-55 55 87 final final ADJ njb-55 55 88     SPACE njb-55 55 89 elongation elongation NOUN njb-55 55 90     SPACE njb-55 55 91 at at ADP njb-55 55 92     SPACE njb-55 55 93 720c 720c NOUN njb-55 55 94     SPACE njb-55 55 95 for for ADP njb-55 55 96     SPACE njb-55 55 97 5 5 NUM njb-55 55 98     SPACE njb-55 55 99 min min PROPN njb-55 55 100 . . PUNCT njb-55 55 101     SPACE njb-55 55 102 finally finally ADV njb-55 55 103     SPACE njb-55 55 104 annealing anneal VERB njb-55 55 105     SPACE njb-55 55 106 temperature temperature NOUN njb-55 55 107     SPACE njb-55 55 108 was be AUX njb-55 55 109     SPACE njb-55 55 110 standardized standardize VERB njb-55 55 111     SPACE njb-55 55 112 to to ADP njb-55 55 113     SPACE njb-55 55 114 600c 600c NOUN njb-55 55 115     SPACE njb-55 55 116 for for ADP njb-55 55 117     SPACE njb-55 55 118 1 1 NUM njb-55 55 119     SPACE njb-55 55 120 min min NOUN njb-55 55 121     SPACE njb-55 55 122 ( ( PUNCT njb-55 55 123 fig fig NOUN njb-55 55 124 . . PUNCT njb-55 55 125     SPACE njb-55 55 126 1b 1b NUM njb-55 55 127 ) ) PUNCT njb-55 55 128 . . PUNCT njb-55 55 129         SPACE njb-55 56 1 optimized optimize VERB njb-55 56 2     SPACE njb-55 56 3 annealing anneal VERB njb-55 56 4     SPACE njb-55 56 5 temperature temperature NOUN njb-55 56 6     SPACE njb-55 56 7 details detail NOUN njb-55 56 8     SPACE njb-55 56 9 shown show VERB njb-55 56 10     SPACE njb-55 56 11 in in ADP njb-55 56 12     SPACE njb-55 56 13 table table NOUN njb-55 56 14     SPACE njb-55 56 15 ( ( PUNCT njb-55 56 16 table table NOUN njb-55 56 17     SPACE njb-55 56 18 2 2 NUM njb-55 56 19 ) ) PUNCT njb-55 56 20 . . PUNCT njb-55 56 21     SPACE njb-55 57 1 agarose agarose NOUN njb-55 57 2     SPACE njb-55 57 3 gel gel NOUN njb-55 57 4     SPACE njb-55 57 5 electrophoresis electrophoresis NOUN njb-55 57 6 . . PUNCT njb-55 57 7     SPACE njb-55 58 1 a a DET njb-55 58 2     SPACE njb-55 58 3 15 15 NUM njb-55 58 4     SPACE njb-55 58 5 ml ml PROPN njb-55 58 6     SPACE njb-55 58 7 aliquot aliquot NOUN njb-55 58 8     SPACE njb-55 58 9 of of ADP njb-55 58 10     SPACE njb-55 58 11 each each DET njb-55 58 12     SPACE njb-55 58 13 pcr pcr NOUN njb-55 58 14     SPACE njb-55 58 15 product product NOUN njb-55 58 16     SPACE njb-55 58 17 was be AUX njb-55 58 18     SPACE njb-55 58 19 mixed mix VERB njb-55 58 20     SPACE njb-55 58 21 with with ADP njb-55 58 22     SPACE njb-55 58 23 3µl 3µl PROPN njb-55 58 24     SPACE njb-55 58 25 of of ADP njb-55 58 26     SPACE njb-55 58 27 6x 6x PROPN njb-55 58 28     SPACE njb-55 58 29 loading loading NOUN njb-55 58 30     SPACE njb-55 58 31 dye dye NOUN njb-55 58 32     SPACE njb-55 58 33 ( ( PUNCT njb-55 58 34 fermentas fermenta NOUN njb-55 58 35 ) ) PUNCT njb-55 58 36     SPACE njb-55 58 37 and and CCONJ njb-55 58 38     SPACE njb-55 58 39 loaded load VERB njb-55 58 40     SPACE njb-55 58 41 on on ADP njb-55 58 42     SPACE njb-55 58 43 to to ADP njb-55 58 44     SPACE njb-55 58 45 the the DET njb-55 58 46     SPACE njb-55 58 47 wells well NOUN njb-55 58 48     SPACE njb-55 58 49 of of ADP njb-55 58 50     SPACE njb-55 58 51 2 2 NUM njb-55 58 52 % % NOUN njb-55 58 53     SPACE njb-55 58 54 agarose agarose NOUN njb-55 58 55     SPACE njb-55 58 56 gel gel NOUN njb-55 58 57     SPACE njb-55 58 58 in in ADP njb-55 58 59     SPACE njb-55 58 60 tae tae NOUN njb-55 58 61     SPACE njb-55 58 62 buffer buffer NOUN njb-55 58 63 . . PUNCT njb-55 58 64     SPACE njb-55 59 1 it it PRON njb-55 59 2     SPACE njb-55 59 3 was be AUX njb-55 59 4     SPACE njb-55 59 5 run run VERB njb-55 59 6     SPACE njb-55 59 7 at at ADP njb-55 59 8     SPACE njb-55 59 9 80v 80v NUM njb-55 59 10     SPACE njb-55 59 11 for for ADP njb-55 59 12     SPACE njb-55 59 13 about about ADV njb-55 59 14     SPACE njb-55 59 15 45 45 NUM njb-55 59 16     SPACE njb-55 59 17 min min PROPN njb-55 59 18 . . PROPN njb-55 59 19     SPACE njb-55 59 20 dna dna PROPN njb-55 59 21     SPACE njb-55 59 22 bands band NOUN njb-55 59 23     SPACE njb-55 59 24 were be AUX njb-55 59 25     SPACE njb-55 59 26 visualized visualize VERB njb-55 59 27     SPACE njb-55 59 28 under under ADP njb-55 59 29     SPACE njb-55 59 30 uv‐trans uv‐tran NOUN njb-55 59 31     SPACE njb-55 59 32 illuminator illuminator NOUN njb-55 59 33     SPACE njb-55 59 34 and and CCONJ njb-55 59 35     SPACE njb-55 59 36 documented document VERB njb-55 59 37     SPACE njb-55 59 38 using use VERB njb-55 59 39     SPACE njb-55 59 40 gel gel NOUN njb-55 59 41     SPACE njb-55 59 42 documentation documentation NOUN njb-55 59 43     SPACE njb-55 59 44 system system NOUN njb-55 59 45     SPACE njb-55 59 46 with with ADP njb-55 59 47     SPACE njb-55 59 48 quantity quantity NOUN njb-55 59 49     SPACE njb-55 59 50 one one NUM njb-55 59 51     SPACE njb-55 59 52 software software NOUN njb-55 59 53     SPACE njb-55 59 54 ( ( PUNCT njb-55 59 55 biorad biorad NOUN njb-55 59 56 , , PUNCT njb-55 59 57     SPACE njb-55 59 58 u.s.a u.s.a NOUN njb-55 59 59 ) ) PUNCT njb-55 59 60     SPACE njb-55 59 61 and and CCONJ njb-55 59 62     SPACE njb-55 59 63 the the DET njb-55 59 64     SPACE njb-55 59 65 pcr pcr NOUN njb-55 59 66     SPACE njb-55 59 67 product product NOUN njb-55 59 68     SPACE njb-55 59 69 was be AUX njb-55 59 70     SPACE njb-55 59 71 eluted elute VERB njb-55 59 72     SPACE njb-55 59 73 using use VERB njb-55 59 74     SPACE njb-55 59 75 eppendorf eppendorf PROPN njb-55 59 76     SPACE njb-55 59 77 qiaquick qiaquick PROPN njb-55 59 78     SPACE njb-55 59 79 gel gel PROPN njb-55 59 80     SPACE njb-55 59 81 extraction extraction NOUN njb-55 59 82     SPACE njb-55 59 83 kit kit NOUN njb-55 59 84     SPACE njb-55 59 85 ( ( PUNCT njb-55 59 86 qiagen qiagen PROPN njb-55 59 87 , , PUNCT njb-55 59 88     SPACE njb-55 59 89 germany germany PROPN njb-55 59 90 ) ) PUNCT njb-55 59 91 . . PUNCT njb-55 59 92     SPACE njb-55 59 93 colonies colony NOUN njb-55 59 94     SPACE njb-55 59 95 were be AUX njb-55 59 96     SPACE njb-55 59 97 suspended suspend VERB njb-55 59 98     SPACE njb-55 59 99 in in ADP njb-55 59 100     SPACE njb-55 59 101 500 500 NUM njb-55 59 102     SPACE njb-55 59 103 µl µl PROPN njb-55 59 104     SPACE njb-55 59 105 te te X njb-55 59 106     SPACE njb-55 59 107 buffer buffer NOUN njb-55 59 108     SPACE njb-55 59 109 or or CCONJ njb-55 59 110     SPACE njb-55 59 111 50µl 50µl PROPN njb-55 59 112     SPACE njb-55 59 113 of of ADP njb-55 59 114     SPACE njb-55 59 115 mid‐log mid‐log NOUN njb-55 59 116     SPACE njb-55 59 117 phase phase NOUN njb-55 59 118     SPACE njb-55 59 119 bacterial bacterial ADJ njb-55 59 120     SPACE njb-55 59 121 culture culture NOUN njb-55 59 122     SPACE njb-55 59 123 was be AUX njb-55 59 124     SPACE njb-55 59 125 added add VERB njb-55 59 126     SPACE njb-55 59 127 to to ADP njb-55 59 128     SPACE njb-55 59 129 450µl 450µl PROPN njb-55 59 130     SPACE njb-55 59 131 of of ADP njb-55 59 132     SPACE njb-55 59 133 te te NOUN njb-55 59 134     SPACE njb-55 59 135 buffer buffer NOUN njb-55 59 136 , , PUNCT njb-55 59 137     SPACE njb-55 59 138 then then ADV njb-55 59 139     SPACE njb-55 59 140 the the DET njb-55 59 141     SPACE njb-55 59 142 cell cell NOUN njb-55 59 143     SPACE njb-55 59 144 suspension suspension NOUN njb-55 59 145     SPACE njb-55 59 146 was be AUX njb-55 59 147     SPACE njb-55 59 148 boiled boil VERB njb-55 59 149     SPACE njb-55 59 150 for for ADP njb-55 59 151     SPACE njb-55 59 152 10 10 NUM njb-55 59 153     SPACE njb-55 59 154 min min NOUN njb-55 59 155     SPACE njb-55 59 156 followed follow VERB njb-55 59 157     SPACE njb-55 59 158 by by ADP njb-55 59 159     SPACE njb-55 59 160 quick quick ADJ njb-55 59 161     SPACE njb-55 59 162 cooling cool VERB njb-55 59 163     SPACE njb-55 59 164 on on ADP njb-55 59 165     SPACE njb-55 59 166 ice ice NOUN njb-55 59 167     SPACE njb-55 59 168 for for ADP njb-55 59 169     SPACE njb-55 59 170 5 5 NUM njb-55 59 171     SPACE njb-55 59 172 min min PROPN njb-55 59 173 , , PUNCT njb-55 59 174     SPACE njb-55 59 175 and and CCONJ njb-55 59 176     SPACE njb-55 59 177 centrifuged centrifuge VERB njb-55 59 178     SPACE njb-55 59 179 at at ADP njb-55 59 180     SPACE njb-55 59 181 12,800 12,800 NUM njb-55 59 182     SPACE njb-55 59 183 g g NOUN njb-55 59 184     SPACE njb-55 59 185 for for ADP njb-55 59 186     SPACE njb-55 59 187 5 5 NUM njb-55 59 188     SPACE njb-55 59 189 min min PROPN njb-55 59 190 . . PUNCT njb-55 59 191         SPACE njb-55 60 1 the the DET njb-55 60 2     SPACE njb-55 60 3 supernatant supernatant NOUN njb-55 60 4     SPACE njb-55 60 5 was be AUX njb-55 60 6     SPACE njb-55 60 7 collected collect VERB njb-55 60 8     SPACE njb-55 60 9 and and CCONJ njb-55 60 10     SPACE njb-55 60 11 stored store VERB njb-55 60 12     SPACE njb-55 60 13 at at ADP njb-55 60 14     SPACE njb-55 60 15 ‐300c ‐300c NOUN njb-55 60 16     SPACE njb-55 60 17 until until ADP njb-55 60 18     SPACE njb-55 60 19 use use NOUN njb-55 60 20 . . PUNCT njb-55 60 21     SPACE njb-55 60 22 primer primer NOUN njb-55 60 23     SPACE njb-55 60 24 design design NOUN njb-55 60 25 . . PUNCT njb-55 60 26     SPACE njb-55 60 27 all all PRON njb-55 60 28     SPACE njb-55 60 29 available available ADJ njb-55 60 30     SPACE njb-55 60 31 sequences sequence NOUN njb-55 60 32     SPACE njb-55 60 33 of of ADP njb-55 60 34     SPACE njb-55 60 35 the the DET njb-55 60 36     SPACE njb-55 60 37 target target NOUN njb-55 60 38     SPACE njb-55 60 39 genes gene NOUN njb-55 60 40     SPACE njb-55 60 41 among among ADP njb-55 60 42     SPACE njb-55 60 43 leptospira leptospira PROPN njb-55 60 44     SPACE njb-55 60 45 spp spp PROPN njb-55 60 46 . . PUNCT njb-55 60 47     SPACE njb-55 60 48 were be AUX njb-55 60 49     SPACE njb-55 60 50 downloaded download VERB njb-55 60 51     SPACE njb-55 60 52 from from ADP njb-55 60 53     SPACE njb-55 60 54 the the DET njb-55 60 55     SPACE njb-55 60 56 genbank genbank PROPN njb-55 60 57     SPACE njb-55 60 58 ( ( PUNCT njb-55 60 59 http://www.ncbi.nlm.nih.gov/genbank/ http://www.ncbi.nlm.nih.gov/genbank/ PROPN njb-55 60 60 genbanksearch.html genbanksearch.html X njb-55 60 61 ) ) PUNCT njb-55 60 62 . . PUNCT njb-55 60 63     SPACE njb-55 61 1 perlprimer perlprimer PROPN njb-55 61 2     SPACE njb-55 61 3 software software NOUN njb-55 61 4     SPACE njb-55 61 5 by by ADP njb-55 61 6     SPACE njb-55 61 7 marshall marshall PROPN njb-55 61 8     SPACE njb-55 61 9 et et PROPN njb-55 61 10     SPACE njb-55 61 11 al al PROPN njb-55 61 12 . . PROPN njb-55 61 13 , , PUNCT njb-55 61 14     SPACE njb-55 61 15 ( ( PUNCT njb-55 61 16 2004 2004 NUM njb-55 61 17 ) ) PUNCT njb-55 61 18     SPACE njb-55 61 19 was be AUX njb-55 61 20     SPACE njb-55 61 21 used use VERB njb-55 61 22     SPACE njb-55 61 23 to to PART njb-55 61 24     SPACE njb-55 61 25 design design VERB njb-55 61 26     SPACE njb-55 61 27 the the DET njb-55 61 28     SPACE njb-55 61 29 primers primer NOUN njb-55 61 30 . . PUNCT njb-55 61 31     SPACE njb-55 62 1 sequence sequence NOUN njb-55 62 2     SPACE njb-55 62 3 for for ADP njb-55 62 4     SPACE njb-55 62 5 lipl21 lipl21 NOUN njb-55 62 6     SPACE njb-55 62 7 holding hold VERB njb-55 62 8     SPACE njb-55 62 9 accession accession NOUN njb-55 62 10     SPACE njb-55 62 11 number number NOUN njb-55 62 12     SPACE njb-55 62 13 ay688426 ay688426 PROPN njb-55 62 14     SPACE njb-55 62 15 and and CCONJ njb-55 62 16     SPACE njb-55 62 17 lipl41 lipl41 PROPN njb-55 62 18     SPACE njb-55 62 19 sequence sequence NOUN njb-55 62 20     SPACE njb-55 62 21 of of ADP njb-55 62 22     SPACE njb-55 62 23 leptospira leptospira PROPN njb-55 62 24     SPACE njb-55 62 25 borgpetersenii borgpetersenii PROPN njb-55 62 26     SPACE njb-55 62 27 serovar serovar NOUN njb-55 62 28     SPACE njb-55 62 29 hardjo‐bovis hardjo‐bovis AUX njb-55 62 30     SPACE njb-55 62 31 holding hold VERB njb-55 62 32     SPACE njb-55 62 33 accession accession NOUN njb-55 62 34     SPACE njb-55 62 35 number number NOUN njb-55 62 36     SPACE njb-55 62 37 cp000348.1 cp000348.1 PRON njb-55 62 38     SPACE njb-55 62 39 was be AUX njb-55 62 40     SPACE njb-55 62 41 retrieved retrieve VERB njb-55 62 42     SPACE njb-55 62 43 from from ADP njb-55 62 44     SPACE njb-55 62 45 ncbi‐genbank ncbi‐genbank PROPN njb-55 62 46 . . PUNCT njb-55 62 47       SPACE njb-55 63 1 it it PRON njb-55 63 2     SPACE njb-55 63 3 was be AUX njb-55 63 4     SPACE njb-55 63 5 then then ADV njb-55 63 6     SPACE njb-55 63 7 used use VERB njb-55 63 8     SPACE njb-55 63 9 to to PART njb-55 63 10     SPACE njb-55 63 11 get get AUX njb-55 63 12     SPACE njb-55 63 13 conserved conserve VERB njb-55 63 14     SPACE njb-55 63 15 sequences sequence NOUN njb-55 63 16 . . PUNCT njb-55 63 17     SPACE njb-55 64 1 the the DET njb-55 64 2     SPACE njb-55 64 3 conserved conserve VERB njb-55 64 4     SPACE njb-55 64 5 part part NOUN njb-55 64 6     SPACE njb-55 64 7 of of ADP njb-55 64 8     SPACE njb-55 64 9 the the DET njb-55 64 10     SPACE njb-55 64 11 nucleotide nucleotide NOUN njb-55 64 12     SPACE njb-55 64 13 sequence sequence NOUN njb-55 64 14     SPACE njb-55 64 15 was be AUX njb-55 64 16     SPACE njb-55 64 17 retrieved retrieve VERB njb-55 64 18     SPACE njb-55 64 19 and and CCONJ njb-55 64 20     SPACE njb-55 64 21 used use VERB njb-55 64 22     SPACE njb-55 64 23 for for ADP njb-55 64 24     SPACE njb-55 64 25 primer primer NOUN njb-55 64 26     SPACE njb-55 64 27 designing designing NOUN njb-55 64 28 . . PUNCT njb-55 64 29     SPACE njb-55 65 1 the the DET njb-55 65 2     SPACE njb-55 65 3 criteria criterion NOUN njb-55 65 4     SPACE njb-55 65 5 that that PRON njb-55 65 6     SPACE njb-55 65 7 was be AUX njb-55 65 8     SPACE njb-55 65 9 set set VERB njb-55 65 10     SPACE njb-55 65 11 for for ADP njb-55 65 12     SPACE njb-55 65 13 primer primer NOUN njb-55 65 14     SPACE njb-55 65 15 design design NOUN njb-55 65 16     SPACE njb-55 65 17 was be AUX njb-55 65 18     SPACE njb-55 65 19 primer primer NOUN njb-55 65 20     SPACE njb-55 65 21 length length NOUN njb-55 65 22     SPACE njb-55 65 23 is be AUX njb-55 65 24     SPACE njb-55 65 25 20±2 20±2 NUM njb-55 65 26     SPACE njb-55 65 27 target target NOUN njb-55 65 28     SPACE njb-55 65 29 temperature temperature NOUN njb-55 65 30     SPACE njb-55 65 31 is be AUX njb-55 65 32     SPACE njb-55 65 33 55±1 55±1 NUM njb-55 65 34 ° ° PUNCT njb-55 66 1 c. c. PROPN njb-55 66 2         SPACE njb-55 66 3 the the DET njb-55 66 4     SPACE njb-55 66 5 overall overall ADJ njb-55 66 6     SPACE njb-55 66 7 theoretical theoretical ADJ njb-55 66 8     SPACE njb-55 66 9 specificities specificity NOUN njb-55 66 10     SPACE njb-55 66 11 of of ADP njb-55 66 12     SPACE njb-55 66 13 the the DET njb-55 66 14     SPACE njb-55 66 15 newly newly ADV njb-55 66 16     SPACE njb-55 66 17 designed design VERB njb-55 66 18     SPACE njb-55 66 19 primers primer NOUN njb-55 66 20     SPACE njb-55 66 21 were be AUX njb-55 66 22     SPACE njb-55 66 23 checked check VERB njb-55 66 24     SPACE njb-55 66 25 using use VERB njb-55 66 26     SPACE njb-55 66 27 blast blast NOUN njb-55 66 28     SPACE njb-55 66 29 search search NOUN njb-55 66 30 . . PUNCT njb-55 66 31       SPACE njb-55 67 1 details detail NOUN njb-55 67 2     SPACE njb-55 67 3 of of ADP njb-55 67 4     SPACE njb-55 67 5 the the DET njb-55 67 6     SPACE njb-55 67 7 primers primer NOUN njb-55 67 8 ’ ’ PART njb-55 67 9     SPACE njb-55 67 10 sequences sequence NOUN njb-55 67 11 , , PUNCT njb-55 67 12     SPACE njb-55 67 13 their their PRON njb-55 67 14     SPACE njb-55 67 15 expected expect VERB njb-55 67 16     SPACE njb-55 67 17 amplicons amplicon NOUN njb-55 67 18     SPACE njb-55 67 19 sizes size NOUN njb-55 67 20     SPACE njb-55 67 21 after after ADP njb-55 67 22     SPACE njb-55 67 23 pcr pcr NOUN njb-55 67 24 , , PUNCT njb-55 67 25     SPACE njb-55 67 26 etc etc X njb-55 67 27 . . PUNCT njb-55 67 28     SPACE njb-55 67 29 are be AUX njb-55 67 30     SPACE njb-55 67 31 summarized summarize VERB njb-55 67 32     SPACE njb-55 67 33 in in ADP njb-55 67 34     SPACE njb-55 67 35 table table NOUN njb-55 67 36     SPACE njb-55 67 37 ( ( PUNCT njb-55 67 38 table table NOUN njb-55 67 39     SPACE njb-55 67 40 1 1 NUM njb-55 67 41 ) ) PUNCT njb-55 67 42 . . PUNCT njb-55 67 43     SPACE njb-55 68 1 standardization standardization NOUN njb-55 68 2     SPACE njb-55 68 3 of of ADP njb-55 68 4     SPACE njb-55 68 5 polymerase polymerase NOUN njb-55 68 6     SPACE njb-55 68 7 chain chain NOUN njb-55 68 8     SPACE njb-55 68 9 reaction reaction NOUN njb-55 68 10     SPACE njb-55 68 11 ( ( PUNCT njb-55 68 12 pcr pcr PROPN njb-55 68 13 ) ) PUNCT njb-55 68 14     SPACE njb-55 68 15 protocol protocol NOUN njb-55 68 16 . . PUNCT njb-55 68 17     SPACE njb-55 69 1 in in ADP njb-55 69 2     SPACE njb-55 69 3 order order NOUN njb-55 69 4     SPACE njb-55 69 5 to to PART njb-55 69 6     SPACE njb-55 69 7 achieve achieve VERB njb-55 69 8     SPACE njb-55 69 9 the the DET njb-55 69 10     SPACE njb-55 69 11 best good ADJ njb-55 69 12     SPACE njb-55 69 13 sensitivity sensitivity NOUN njb-55 69 14     SPACE njb-55 69 15 for for ADP njb-55 69 16     SPACE njb-55 69 17 the the DET njb-55 69 18     SPACE njb-55 69 19 pcr pcr NOUN njb-55 69 20     SPACE njb-55 69 21 using use VERB njb-55 69 22     SPACE njb-55 69 23 primer primer NOUN njb-55 69 24     SPACE njb-55 69 25 p28/29 p28/29 VERB njb-55 69 26     SPACE njb-55 69 27 several several ADJ njb-55 69 28     SPACE njb-55 69 29 combinations combination NOUN njb-55 69 30     SPACE njb-55 69 31 of of ADP njb-55 69 32     SPACE njb-55 69 33 annealing anneal VERB njb-55 69 34     SPACE njb-55 69 35 temperatures temperature NOUN njb-55 69 36     SPACE njb-55 69 37 from from ADP njb-55 69 38     SPACE njb-55 69 39 580c 580c NOUN njb-55 69 40     SPACE njb-55 69 41 to to ADP njb-55 69 42     SPACE njb-55 69 43 680c 680c PROPN njb-55 69 44     SPACE njb-55 69 45 were be AUX njb-55 69 46     SPACE njb-55 69 47 tested test VERB njb-55 69 48 . . PUNCT njb-55 69 49     SPACE njb-55 70 1 the the DET njb-55 70 2     SPACE njb-55 70 3 following follow VERB njb-55 70 4     SPACE njb-55 70 5 conditions condition NOUN njb-55 70 6     SPACE njb-55 70 7 were be AUX njb-55 70 8     SPACE njb-55 70 9 chosen choose VERB njb-55 70 10 : : PUNCT njb-55 70 11     SPACE njb-55 70 12 pcr pcr PROPN njb-55 70 13     SPACE njb-55 70 14 was be AUX njb-55 70 15     SPACE njb-55 70 16 performed perform VERB njb-55 70 17     SPACE njb-55 70 18 in in ADP njb-55 70 19     SPACE njb-55 70 20 25 25 NUM njb-55 70 21     SPACE njb-55 70 22 ml ml PROPN njb-55 70 23     SPACE njb-55 70 24 reaction reaction NOUN njb-55 70 25     SPACE njb-55 70 26 volume volume NOUN njb-55 70 27     SPACE njb-55 70 28 containing contain VERB njb-55 70 29     SPACE njb-55 70 30 10x 10x PROPN njb-55 70 31     SPACE njb-55 70 32 taq taq PROPN njb-55 70 33     SPACE njb-55 70 34 buffer buffer NOUN njb-55 70 35     SPACE njb-55 70 36 with with ADP njb-55 70 37     SPACE njb-55 70 38 kcl kcl PROPN njb-55 70 39     SPACE njb-55 70 40 ( ( PUNCT njb-55 70 41 100 100 NUM njb-55 70 42 mm mm PROPN njb-55 70 43     SPACE njb-55 70 44 tris tris PROPN njb-55 70 45     SPACE njb-55 70 46 hcl hcl PROPN njb-55 70 47 , , PUNCT njb-55 70 48     SPACE njb-55 70 49 50 50 NUM njb-55 70 50 mm mm NOUN njb-55 70 51     SPACE njb-55 70 52 kcl kcl PROPN njb-55 70 53 , , PUNCT njb-55 70 54     SPACE njb-55 70 55 0.8 0.8 NUM njb-55 70 56 % % NOUN njb-55 70 57     SPACE njb-55 70 58 nonidet nonidet NOUN njb-55 70 59     SPACE njb-55 70 60 p40 p40 NOUN njb-55 70 61 ) ) PUNCT njb-55 70 62 , , PUNCT njb-55 70 63     SPACE njb-55 70 64 1.5 1.5 NUM njb-55 70 65 mm mm PROPN njb-55 70 66     SPACE njb-55 70 67 mgcl2 mgcl2 PROPN njb-55 70 68 , , PUNCT njb-55 70 69     SPACE njb-55 70 70 2.5 2.5 NUM njb-55 70 71 mm mm NOUN njb-55 70 72     SPACE njb-55 70 73 of of ADP njb-55 70 74     SPACE njb-55 70 75 each each DET njb-55 70 76     SPACE njb-55 70 77 dntps dntps NOUN njb-55 70 78 , , PUNCT njb-55 70 79     SPACE njb-55 70 80 2u 2u NUM njb-55 70 81     SPACE njb-55 70 82 of of ADP njb-55 70 83     SPACE njb-55 70 84 taq taq PROPN njb-55 70 85     SPACE njb-55 70 86 polymerase polymerase NOUN njb-55 70 87 , , PUNCT njb-55 70 88     SPACE njb-55 70 89 25pmoles 25pmoles NUM njb-55 70 90     SPACE njb-55 70 91 of of ADP njb-55 70 92     SPACE njb-55 70 93 each each DET njb-55 70 94     SPACE njb-55 70 95 primer primer NOUN njb-55 70 96     SPACE njb-55 70 97 and and CCONJ njb-55 70 98     SPACE njb-55 70 99 0.25ml 0.25ml NOUN njb-55 70 100     SPACE njb-55 70 101 of of ADP njb-55 70 102     SPACE njb-55 70 103 dna dna NOUN njb-55 70 104     SPACE njb-55 70 105 ( ( PUNCT njb-55 70 106 40ng 40ng NOUN njb-55 70 107 / / SYM njb-55 70 108 ml ml PROPN njb-55 70 109 ) ) PUNCT njb-55 70 110     SPACE njb-55 70 111 extract extract NOUN njb-55 70 112     SPACE njb-55 70 113 from from ADP njb-55 70 114     SPACE njb-55 70 115 l. l. NOUN njb-55 70 116     SPACE njb-55 70 117 icterohaemorrhagiae icterohaemorrhagiae NOUN njb-55 70 118 . . PUNCT njb-55 70 119     SPACE njb-55 71 1 pcr pcr PROPN njb-55 71 2     SPACE njb-55 71 3 chemicals chemical NOUN njb-55 71 4     SPACE njb-55 71 5 were be AUX njb-55 71 6     SPACE njb-55 71 7 procured procure VERB njb-55 71 8     SPACE njb-55 71 9 from from ADP njb-55 71 10     SPACE njb-55 71 11 fermantas fermanta NOUN njb-55 71 12 . . PUNCT njb-55 71 13     SPACE njb-55 72 1 the the DET njb-55 72 2     SPACE njb-55 72 3 reactions reaction NOUN njb-55 72 4     SPACE njb-55 72 5 were be AUX njb-55 72 6     SPACE njb-55 72 7 carried carry VERB njb-55 72 8     SPACE njb-55 72 9 out out ADP njb-55 72 10     SPACE njb-55 72 11 in in ADP njb-55 72 12     SPACE njb-55 72 13 an an DET njb-55 72 14     SPACE njb-55 72 15 eppendorf eppendorf PROPN njb-55 72 16     SPACE njb-55 72 17 thermocycler thermocycler PROPN njb-55 72 18 . . PUNCT njb-55 72 19     SPACE njb-55 73 1 initially initially ADV njb-55 73 2 , , PUNCT njb-55 73 3     SPACE njb-55 73 4 pcr pcr NOUN njb-55 73 5     SPACE njb-55 73 6 conditions condition NOUN njb-55 73 7     SPACE njb-55 73 8 were be AUX njb-55 73 9     SPACE njb-55 73 10 optimized optimize VERB njb-55 73 11     SPACE njb-55 73 12 by by ADP njb-55 73 13     SPACE njb-55 73 14 gradient gradient ADJ njb-55 73 15     SPACE njb-55 73 16 pcr pcr PROPN njb-55 73 17 . . PUNCT njb-55 73 18     SPACE njb-55 74 1 the the DET njb-55 74 2     SPACE njb-55 74 3 reaction reaction NOUN njb-55 74 4     SPACE njb-55 74 5 started start VERB njb-55 74 6     SPACE njb-55 74 7 with with ADP njb-55 74 8     SPACE njb-55 74 9 an an DET njb-55 74 10     SPACE njb-55 74 11 initial initial ADJ njb-55 74 12     SPACE njb-55 74 13 polymerase polymerase NOUN njb-55 74 14     SPACE njb-55 74 15 activating activate VERB njb-55 74 16     SPACE njb-55 74 17 temperature temperature NOUN njb-55 74 18     SPACE njb-55 74 19 of of ADP njb-55 74 20     SPACE njb-55 74 21 950c 950c PROPN njb-55 74 22     SPACE njb-55 74 23 for for ADP njb-55 74 24     SPACE njb-55 74 25 5 5 NUM njb-55 74 26     SPACE njb-55 74 27 min min NOUN njb-55 74 28     SPACE njb-55 74 29 followed follow VERB njb-55 74 30     SPACE njb-55 74 31 35 35 NUM njb-55 74 32     SPACE njb-55 74 33 cycles cycle NOUN njb-55 74 34     SPACE njb-55 74 35 of of ADP njb-55 74 36     SPACE njb-55 74 37 denaturing denature VERB njb-55 74 38     SPACE njb-55 74 39 at at ADP njb-55 74 40     SPACE njb-55 74 41 950c 950c PROPN njb-55 74 42     SPACE njb-55 74 43 for for ADP njb-55 74 44     SPACE njb-55 74 45 1 1 NUM njb-55 74 46     SPACE njb-55 74 47 min min NOUN njb-55 74 48 , , PUNCT njb-55 74 49     SPACE njb-55 74 50 annealing anneal VERB njb-55 74 51     SPACE njb-55 74 52 at at ADP njb-55 74 53     SPACE njb-55 74 54 630c 630c NOUN njb-55 74 55     SPACE njb-55 74 56 for for ADP njb-55 74 57     SPACE njb-55 74 58 1 1 NUM njb-55 74 59     SPACE njb-55 74 60 min min NOUN njb-55 74 61     SPACE njb-55 74 62 at at ADP njb-55 74 63     SPACE njb-55 74 64 g g NOUN njb-55 74 65     SPACE njb-55 74 66 ± ± NOUN njb-55 74 67     SPACE njb-55 74 68 50c 50c NOUN njb-55 74 69 , , PUNCT njb-55 74 70     SPACE njb-55 74 71 elongation elongation NOUN njb-55 74 72     SPACE njb-55 74 73 primer primer NOUN njb-55 74 74     SPACE njb-55 74 75 target target NOUN njb-55 74 76     SPACE njb-55 74 77 species species PROPN njb-55 74 78 / / SYM njb-55 74 79 gene gene NOUN njb-55 74 80     SPACE njb-55 74 81 sequence(5’‐3 sequence(5’‐3 PROPN njb-55 74 82 ’ ' PUNCT njb-55 74 83 ) ) PUNCT njb-55 74 84     SPACE njb-55 74 85 amplicon amplicon NOUN njb-55 74 86     SPACE njb-55 74 87 length length NOUN njb-55 74 88     SPACE njb-55 74 89 reference reference NOUN njb-55 74 90     SPACE njb-55 74 91 ( ( PUNCT njb-55 74 92 accession accession NOUN njb-55 74 93     SPACE njb-55 74 94 no no INTJ njb-55 74 95 . . PUNCT njb-55 74 96 ) ) PUNCT njb-55 74 97     SPACE njb-55 75 1 p28 p28 NOUN njb-55 75 2 / / SYM njb-55 75 3 f f PROPN njb-55 75 4     SPACE njb-55 75 5 leptospira leptospira PROPN njb-55 75 6 / / SYM njb-55 75 7 lipl21 lipl21 PROPN njb-55 75 8     SPACE njb-55 75 9 ccagcactgacaccggacaaa ccagcactgacaccggacaaa NOUN njb-55 75 10     SPACE njb-55 75 11 385bp 385bp PROPN njb-55 75 12     SPACE njb-55 75 13 ay688426 ay688426 PROPN njb-55 75 14     SPACE njb-55 75 15 p29 p29 PROPN njb-55 75 16 / / SYM njb-55 75 17 r r NOUN njb-55 75 18     SPACE njb-55 75 19 leptospira leptospira PROPN njb-55 75 20 / / SYM njb-55 75 21 lipl21 lipl21 PROPN njb-55 75 22     SPACE njb-55 75 23 ccggaaccaaccgctttacat ccggaaccaaccgctttacat NOUN njb-55 75 24     SPACE njb-55 75 25 385bp 385bp PROPN njb-55 75 26     SPACE njb-55 75 27 ay688426 ay688426 PROPN njb-55 75 28     SPACE njb-55 75 29 p30 p30 PROPN njb-55 75 30 / / SYM njb-55 75 31 f f NOUN njb-55 75 32     SPACE njb-55 75 33 leptospira leptospira PROPN njb-55 75 34 / / SYM njb-55 75 35 lipl41 lipl41 PROPN njb-55 75 36     SPACE njb-55 75 37 gacctcagtaaacgcgccgatat gacctcagtaaacgcgccgatat PROPN njb-55 75 38     SPACE njb-55 75 39 427bp 427bp PROPN njb-55 75 40     SPACE njb-55 75 41 cp000348.1 cp000348.1 SYM njb-55 75 42     SPACE njb-55 75 43 p31 p31 PROPN njb-55 75 44 / / SYM njb-55 75 45 r r NOUN njb-55 75 46     SPACE njb-55 75 47 leptospira leptospira PROPN njb-55 75 48 / / SYM njb-55 75 49 lipl41 lipl41 PROPN njb-55 75 50     SPACE njb-55 76 1 cagcggcttcgtccaatcct cagcggcttcgtccaatcct PROPN njb-55 76 2     SPACE njb-55 76 3 427bp 427bp NOUN njb-55 76 4     SPACE njb-55 76 5 cp000348.1 cp000348.1 SYM njb-55 76 6     SPACE njb-55 76 7 table table NOUN njb-55 76 8     SPACE njb-55 76 9 1 1 NUM njb-55 76 10 . . PUNCT njb-55 76 11     SPACE njb-55 77 1 details detail NOUN njb-55 77 2     SPACE njb-55 77 3 of of ADP njb-55 77 4     SPACE njb-55 77 5 the the DET njb-55 77 6     SPACE njb-55 77 7 newly newly ADV njb-55 77 8     SPACE njb-55 77 9 designed design VERB njb-55 77 10     SPACE njb-55 77 11 primers primer NOUN njb-55 77 12     SPACE njb-55 77 13 for for ADP njb-55 77 14     SPACE njb-55 77 15 specific specific ADJ njb-55 77 16     SPACE njb-55 77 17 identification identification NOUN njb-55 77 18           SPACE njb-55 77 19 pcr pcr ADJ njb-55 77 20     SPACE njb-55 77 21 reaction reaction NOUN njb-55 77 22     SPACE njb-55 77 23 mixture(25ml mixture(25ml PROPN njb-55 77 24 ) ) PUNCT njb-55 77 25           SPACE njb-55 77 26 p28 p28 NOUN njb-55 77 27     SPACE njb-55 77 28 /p29 /p29 PUNCT njb-55 77 29           SPACE njb-55 77 30 p30 p30 NOUN njb-55 77 31 / / SYM njb-55 77 32 p31 p31 PROPN njb-55 77 33           SPACE njb-55 77 34 10x 10x NOUN njb-55 77 35     SPACE njb-55 77 36 buffer buffer NOUN njb-55 77 37                                                     SPACE njb-55 77 38 2.50ml 2.50ml NUM njb-55 77 39     SPACE njb-55 77 40 mgcl2 mgcl2 PROPN njb-55 77 41                                                                                                       SPACE njb-55 77 42 1.50ml 1.50ml NUM njb-55 77 43     SPACE njb-55 77 44 dntps dntps NOUN njb-55 77 45                                                                                                       SPACE njb-55 77 46 2.00ml 2.00ml NUM njb-55 77 47     SPACE njb-55 77 48 primer‐forward primer‐forward NOUN njb-55 77 49                                                   SPACE njb-55 77 50 0.50ml 0.50ml NUM njb-55 77 51     SPACE njb-55 77 52 primer‐reverse primer‐reverse NOUN njb-55 77 53                                                     SPACE njb-55 77 54 0.50ml 0.50ml NUM njb-55 77 55     SPACE njb-55 77 56 template template NOUN njb-55 77 57                                                                                       SPACE njb-55 77 58 0.25ml 0.25ml PROPN njb-55 77 59     SPACE njb-55 77 60 taq taq PROPN njb-55 77 61     SPACE njb-55 77 62 pol pol PROPN njb-55 77 63                                                                           SPACE njb-55 77 64 2.00ml 2.00ml NUM njb-55 77 65     SPACE njb-55 77 66 nuclease nuclease ADJ njb-55 77 67         SPACE njb-55 77 68 free free ADJ njb-55 77 69     SPACE njb-55 77 70 water water NOUN njb-55 77 71             SPACE njb-55 77 72 15.75µl 15.75µl NUM njb-55 77 73     SPACE njb-55 77 74 table table NOUN njb-55 77 75     SPACE njb-55 77 76 2 2 NUM njb-55 77 77 . . PUNCT njb-55 77 78     SPACE njb-55 78 1 details detail NOUN njb-55 78 2     SPACE njb-55 78 3 of of ADP njb-55 78 4     SPACE njb-55 78 5 standardized standardize VERB njb-55 78 6     SPACE njb-55 78 7 thermal thermal ADJ njb-55 78 8     SPACE njb-55 78 9 cycling cycling NOUN njb-55 78 10     SPACE njb-55 78 11 condition condition NOUN njb-55 78 12     SPACE njb-55 78 13 nepal nepal ADJ njb-55 78 14     SPACE njb-55 78 15 journal journal NOUN njb-55 78 16     SPACE njb-55 78 17 of of ADP njb-55 78 18     SPACE njb-55 78 19 biotechnology biotechnology NOUN njb-55 78 20 . . PUNCT njb-55 78 21     SPACE njb-55 79 1 jan jan PROPN njb-55 79 2 . . PROPN njb-55 79 3     SPACE njb-55 79 4 2011 2011 NUM njb-55 79 5 , , PUNCT njb-55 79 6     SPACE njb-55 79 7 vol vol NOUN njb-55 79 8 . . PUNCT njb-55 79 9     SPACE njb-55 79 10 1 1 NUM njb-55 79 11 , , PUNCT njb-55 79 12     SPACE njb-55 79 13 no no INTJ njb-55 79 14 . . PUNCT njb-55 79 15     SPACE njb-55 79 16 1 1 NUM njb-55 79 17 : : PUNCT njb-55 79 18     SPACE njb-55 79 19 22‐30 22‐30 NUM njb-55 79 20     SPACE njb-55 79 21 25 25 NUM njb-55 79 22     SPACE njb-55 79 23 biotechnology biotechnology NOUN njb-55 79 24     SPACE njb-55 79 25 society society NOUN njb-55 79 26     SPACE njb-55 79 27 of of ADP njb-55 79 28     SPACE njb-55 79 29 nepal nepal NOUN njb-55 79 30     SPACE njb-55 79 31 ( ( PUNCT njb-55 79 32 bsn bsn PROPN njb-55 79 33 ) ) PUNCT njb-55 79 34 , , PUNCT njb-55 79 35     SPACE njb-55 79 36 all all DET njb-55 79 37     SPACE njb-55 79 38 rights right NOUN njb-55 79 39     SPACE njb-55 79 40 reserved reserve VERB njb-55 79 41       SPACE njb-55 79 42 min min PROPN njb-55 79 43 . . PUNCT njb-55 79 44     SPACE njb-55 79 45 immediately immediately ADV njb-55 79 46     SPACE njb-55 79 47 mixture mixture NOUN njb-55 79 48       SPACE njb-55 79 49 was be AUX njb-55 79 50     SPACE njb-55 79 51 kept keep VERB njb-55 79 52     SPACE njb-55 79 53 over over ADP njb-55 79 54     SPACE njb-55 79 55 ice ice NOUN njb-55 79 56     SPACE njb-55 79 57 for for ADP njb-55 79 58     SPACE njb-55 79 59 2min 2min NUM njb-55 79 60 . . PUNCT njb-55 79 61       SPACE njb-55 80 1 0.8ml 0.8ml PROPN njb-55 80 2     SPACE njb-55 80 3 of of ADP njb-55 80 4     SPACE njb-55 80 5 lb lb NOUN njb-55 80 6     SPACE njb-55 80 7 broth broth NOUN njb-55 80 8     SPACE njb-55 80 9 was be AUX njb-55 80 10     SPACE njb-55 80 11 then then ADV njb-55 80 12     SPACE njb-55 80 13 added add VERB njb-55 80 14     SPACE njb-55 80 15 to to ADP njb-55 80 16     SPACE njb-55 80 17 it it PRON njb-55 80 18     SPACE njb-55 80 19 and and CCONJ njb-55 80 20     SPACE njb-55 80 21 incubated incubate VERB njb-55 80 22     SPACE njb-55 80 23 at at ADP njb-55 80 24     SPACE njb-55 80 25 37 37 NUM njb-55 80 26 ° ° NOUN njb-55 81 1 c c NOUN njb-55 81 2     SPACE njb-55 81 3 for for ADP njb-55 81 4     SPACE njb-55 81 5 90 90 NUM njb-55 81 6     SPACE njb-55 81 7 min min NOUN njb-55 81 8     SPACE njb-55 81 9 in in ADP njb-55 81 10     SPACE njb-55 81 11 an an DET njb-55 81 12     SPACE njb-55 81 13 orbital orbital ADJ njb-55 81 14     SPACE njb-55 81 15 shaker shaker NOUN njb-55 81 16 . . PUNCT njb-55 81 17         SPACE njb-55 82 1 it it PRON njb-55 82 2     SPACE njb-55 82 3 was be AUX njb-55 82 4     SPACE njb-55 82 5 then then ADV njb-55 82 6     SPACE njb-55 82 7 centrifuged centrifuge VERB njb-55 82 8     SPACE njb-55 82 9 at at ADP njb-55 82 10     SPACE njb-55 82 11 4000 4000 NUM njb-55 82 12     SPACE njb-55 82 13 rpm rpm NOUN njb-55 82 14     SPACE njb-55 82 15 for for ADP njb-55 82 16     SPACE njb-55 82 17 10 10 NUM njb-55 82 18     SPACE njb-55 82 19 min min NOUN njb-55 82 20     SPACE njb-55 82 21 and and CCONJ njb-55 82 22     SPACE njb-55 82 23 the the DET njb-55 82 24     SPACE njb-55 82 25 pellet pellet NOUN njb-55 82 26     SPACE njb-55 82 27 was be AUX njb-55 82 28     SPACE njb-55 82 29 plated plate VERB njb-55 82 30     SPACE njb-55 82 31 on on ADP njb-55 82 32     SPACE njb-55 82 33 lb lb NOUN njb-55 82 34     SPACE njb-55 82 35 agar agar NOUN njb-55 82 36     SPACE njb-55 82 37 plate plate NOUN njb-55 82 38     SPACE njb-55 82 39 containing contain VERB njb-55 82 40     SPACE njb-55 82 41 ampicillin ampicillin NOUN njb-55 82 42     SPACE njb-55 82 43 ( ( PUNCT njb-55 82 44 50mg 50mg PROPN njb-55 82 45 / / SYM njb-55 82 46 ml ml PROPN njb-55 82 47 ) ) PUNCT njb-55 82 48     SPACE njb-55 82 49 and and CCONJ njb-55 82 50     SPACE njb-55 82 51 40ml 40ml NOUN njb-55 82 52     SPACE njb-55 82 53 of of ADP njb-55 82 54     SPACE njb-55 82 55 2 2 NUM njb-55 82 56 % % NOUN njb-55 82 57     SPACE njb-55 82 58 x‐gal x‐gal PROPN njb-55 82 59     SPACE njb-55 82 60 ( ( PUNCT njb-55 82 61 5‐bromo‐4‐chloro‐3‐indolyl‐b‐ 5‐bromo‐4‐chloro‐3‐indolyl‐b‐ NUM njb-55 82 62 galactopyranoside galactopyranoside NOUN njb-55 82 63 ) ) PUNCT njb-55 82 64     SPACE njb-55 82 65 and and CCONJ njb-55 82 66     SPACE njb-55 82 67 8ml 8ml NOUN njb-55 82 68     SPACE njb-55 82 69 of of ADP njb-55 82 70     SPACE njb-55 82 71 2 2 NUM njb-55 82 72 % % NOUN njb-55 82 73     SPACE njb-55 82 74 iptg iptg NOUN njb-55 82 75     SPACE njb-55 82 76 ( ( PUNCT njb-55 82 77 isopropyl‐b‐d isopropyl‐b‐d PROPN njb-55 82 78 – – PUNCT njb-55 82 79 thiogalactopyranoside thiogalactopyranoside NOUN njb-55 82 80 ) ) PUNCT njb-55 82 81 . . PUNCT njb-55 82 82     SPACE njb-55 83 1 the the DET njb-55 83 2     SPACE njb-55 83 3 plates plate NOUN njb-55 83 4     SPACE njb-55 83 5 were be AUX njb-55 83 6     SPACE njb-55 83 7 then then ADV njb-55 83 8     SPACE njb-55 83 9 incubated incubate VERB njb-55 83 10     SPACE njb-55 83 11 over over ADP njb-55 83 12     SPACE njb-55 83 13 night night NOUN njb-55 83 14     SPACE njb-55 83 15 at at ADP njb-55 83 16     SPACE njb-55 83 17 37 37 NUM njb-55 83 18 ° ° NOUN njb-55 83 19 c c NOUN njb-55 83 20 . . PUNCT njb-55 83 21     SPACE njb-55 84 1 well‐isolated well‐isolate VERB njb-55 84 2     SPACE njb-55 84 3 white white PROPN njb-55 84 4     SPACE njb-55 84 5 colonies colony NOUN njb-55 84 6     SPACE njb-55 84 7 ( ( PUNCT njb-55 84 8 fig fig NOUN njb-55 84 9 . . PUNCT njb-55 84 10     SPACE njb-55 84 11 4 4 X njb-55 84 12 ) ) PUNCT njb-55 84 13     SPACE njb-55 84 14 were be AUX njb-55 84 15     SPACE njb-55 84 16 picked pick VERB njb-55 84 17     SPACE njb-55 84 18 and and CCONJ njb-55 84 19     SPACE njb-55 84 20 transferred transfer VERB njb-55 84 21     SPACE njb-55 84 22 to to ADP njb-55 84 23     SPACE njb-55 84 24 5ml 5ml PROPN njb-55 84 25     SPACE njb-55 84 26 of of ADP njb-55 84 27     SPACE njb-55 84 28 lb lb NOUN njb-55 84 29     SPACE njb-55 84 30 broth broth NOUN njb-55 84 31     SPACE njb-55 84 32 containing contain VERB njb-55 84 33     SPACE njb-55 84 34 5ml 5ml PROPN njb-55 84 35     SPACE njb-55 84 36 of of ADP njb-55 84 37     SPACE njb-55 84 38 ampicillin ampicillin NOUN njb-55 84 39     SPACE njb-55 84 40 ( ( PUNCT njb-55 84 41 50mg 50mg PROPN njb-55 84 42 / / SYM njb-55 84 43 ml ml PROPN njb-55 84 44 ) ) PUNCT njb-55 84 45 . . PUNCT njb-55 84 46       SPACE njb-55 85 1 the the DET njb-55 85 2     SPACE njb-55 85 3 tubes tube NOUN njb-55 85 4     SPACE njb-55 85 5 were be AUX njb-55 85 6     SPACE njb-55 85 7 then then ADV njb-55 85 8     SPACE njb-55 85 9 incubated incubate VERB njb-55 85 10     SPACE njb-55 85 11 at at ADP njb-55 85 12     SPACE njb-55 85 13 37 37 NUM njb-55 85 14 ° ° NOUN njb-55 85 15 c c NOUN njb-55 85 16     SPACE njb-55 85 17 in in ADP njb-55 85 18     SPACE njb-55 85 19 the the DET njb-55 85 20     SPACE njb-55 85 21 shaker shaker NOUN njb-55 85 22     SPACE njb-55 85 23 for for ADP njb-55 85 24     SPACE njb-55 85 25 overnight overnight ADV njb-55 85 26 . . PUNCT njb-55 85 27     SPACE njb-55 86 1 plasmid plasmid PROPN njb-55 86 2     SPACE njb-55 86 3 dna dna PROPN njb-55 86 4     SPACE njb-55 86 5 isolation isolation NOUN njb-55 86 6     SPACE njb-55 86 7 and and CCONJ njb-55 86 8     SPACE njb-55 86 9 purification purification NOUN njb-55 86 10     SPACE njb-55 86 11 was be AUX njb-55 86 12     SPACE njb-55 86 13 done do VERB njb-55 86 14     SPACE njb-55 86 15 using use VERB njb-55 86 16     SPACE njb-55 86 17 qiaprep qiaprep PROPN njb-55 86 18     SPACE njb-55 86 19 spin spin PROPN njb-55 86 20     SPACE njb-55 86 21 miniprep miniprep PROPN njb-55 86 22     SPACE njb-55 86 23 kit kit NOUN njb-55 86 24     SPACE njb-55 86 25 ( ( PUNCT njb-55 86 26 qiagen qiagen PROPN njb-55 86 27 , , PUNCT njb-55 86 28     SPACE njb-55 86 29 germany germany PROPN njb-55 86 30 ) ) PUNCT njb-55 86 31     SPACE njb-55 86 32 and and CCONJ njb-55 86 33     SPACE njb-55 86 34 microcentrifuge microcentrifuge NOUN njb-55 86 35 . . PUNCT njb-55 86 36     SPACE njb-55 87 1 characterization characterization NOUN njb-55 87 2     SPACE njb-55 87 3 of of ADP njb-55 87 4     SPACE njb-55 87 5 recombinants recombinant NOUN njb-55 87 6 . . PUNCT njb-55 87 7     SPACE njb-55 88 1 the the DET njb-55 88 2     SPACE njb-55 88 3 plasmids plasmid NOUN njb-55 88 4     SPACE njb-55 88 5 isolated isolate VERB njb-55 88 6     SPACE njb-55 88 7 from from ADP njb-55 88 8     SPACE njb-55 88 9 the the DET njb-55 88 10     SPACE njb-55 88 11 white white ADJ njb-55 88 12     SPACE njb-55 88 13 colonies colony NOUN njb-55 88 14     SPACE njb-55 88 15 were be AUX njb-55 88 16     SPACE njb-55 88 17 further far ADV njb-55 88 18     SPACE njb-55 88 19 characterized characterize VERB njb-55 88 20     SPACE njb-55 88 21 by by ADP njb-55 88 22     SPACE njb-55 88 23 the the DET njb-55 88 24     SPACE njb-55 88 25 insert insert ADJ njb-55 88 26     SPACE njb-55 88 27 release release NOUN njb-55 88 28     SPACE njb-55 88 29 after after ADP njb-55 88 30     SPACE njb-55 88 31 digestion digestion NOUN njb-55 88 32     SPACE njb-55 88 33 with with ADP njb-55 88 34     SPACE njb-55 88 35 ecori ecori PROPN njb-55 88 36     SPACE njb-55 88 37 ( ( PUNCT njb-55 88 38 g g PROPN njb-55 88 39     SPACE njb-55 88 40 ˉaattc ˉaattc PROPN njb-55 88 41 ) ) PUNCT njb-55 88 42     SPACE njb-55 88 43 restriction restriction NOUN njb-55 88 44     SPACE njb-55 88 45 enzyme enzyme NOUN njb-55 88 46 . . PUNCT njb-55 88 47     SPACE njb-55 89 1 the the DET njb-55 89 2     SPACE njb-55 89 3 enzyme enzyme NOUN njb-55 89 4     SPACE njb-55 89 5 ecori ecori PROPN njb-55 89 6     SPACE njb-55 89 7 was be AUX njb-55 89 8     SPACE njb-55 89 9 used use VERB njb-55 89 10     SPACE njb-55 89 11 to to PART njb-55 89 12     SPACE njb-55 89 13 cleave cleave VERB njb-55 89 14     SPACE njb-55 89 15 and and CCONJ njb-55 89 16     SPACE njb-55 89 17 release release VERB njb-55 89 18     SPACE njb-55 89 19 the the DET njb-55 89 20     SPACE njb-55 89 21 insert insert NOUN njb-55 89 22     SPACE njb-55 89 23 in in ADP njb-55 89 24     SPACE njb-55 89 25 the the DET njb-55 89 26     SPACE njb-55 89 27 plasmid plasmid PROPN njb-55 89 28     SPACE njb-55 89 29 ( ( PUNCT njb-55 89 30 fig fig NOUN njb-55 89 31 . . PUNCT njb-55 89 32     SPACE njb-55 90 1 5a 5a NUM njb-55 90 2     SPACE njb-55 90 3 and and CCONJ njb-55 90 4     SPACE njb-55 90 5 5b 5b NUM njb-55 90 6 ) ) PUNCT njb-55 90 7 . . PUNCT njb-55 90 8     SPACE njb-55 91 1 the the DET njb-55 91 2     SPACE njb-55 91 3 reaction reaction NOUN njb-55 91 4     SPACE njb-55 91 5 mixtures mixture NOUN njb-55 91 6     SPACE njb-55 91 7 were be AUX njb-55 91 8     SPACE njb-55 91 9 prepared prepare VERB njb-55 91 10     SPACE njb-55 91 11 as as ADV njb-55 91 12     SPACE njb-55 91 13 per per ADP njb-55 91 14     SPACE njb-55 91 15 the the DET njb-55 91 16     SPACE njb-55 91 17 table table NOUN njb-55 91 18     SPACE njb-55 91 19 below below ADP njb-55 91 20     SPACE njb-55 91 21 and and CCONJ njb-55 91 22     SPACE njb-55 91 23 were be AUX njb-55 91 24     SPACE njb-55 91 25 subjected subject VERB njb-55 91 26     SPACE njb-55 91 27 to to ADP njb-55 91 28     SPACE njb-55 91 29 digestion digestion NOUN njb-55 91 30     SPACE njb-55 91 31 at at ADP njb-55 91 32     SPACE njb-55 91 33 37 37 NUM njb-55 91 34 ° ° NOUN njb-55 91 35 c c NOUN njb-55 91 36     SPACE njb-55 91 37 in in ADP njb-55 91 38     SPACE njb-55 91 39 a a DET njb-55 91 40     SPACE njb-55 91 41 water water NOUN njb-55 91 42     SPACE njb-55 91 43 bath bath NOUN njb-55 91 44     SPACE njb-55 91 45 for for ADP njb-55 91 46     SPACE njb-55 91 47 1h 1h NUM njb-55 91 48 . . PUNCT njb-55 91 49       SPACE njb-55 92 1 details detail NOUN njb-55 92 2     SPACE njb-55 92 3 of of ADP njb-55 92 4     SPACE njb-55 92 5 the the DET njb-55 92 6     SPACE njb-55 92 7 reaction reaction NOUN njb-55 92 8     SPACE njb-55 92 9 mixture mixture NOUN njb-55 92 10     SPACE njb-55 92 11 are be AUX njb-55 92 12     SPACE njb-55 92 13 summarized summarize VERB njb-55 92 14     SPACE njb-55 92 15 in in ADP njb-55 92 16     SPACE njb-55 92 17 table table NOUN njb-55 92 18     SPACE njb-55 92 19 ( ( PUNCT njb-55 92 20 table table NOUN njb-55 92 21     SPACE njb-55 92 22 5 5 NUM njb-55 92 23 ) ) PUNCT njb-55 92 24 . . PUNCT njb-55 92 25     SPACE njb-55 93 1 pcr pcr NOUN njb-55 93 2     SPACE njb-55 93 3 for for ADP njb-55 93 4     SPACE njb-55 93 5 confirmation confirmation NOUN njb-55 93 6 . . PUNCT njb-55 93 7     SPACE njb-55 94 1 the the DET njb-55 94 2     SPACE njb-55 94 3 pcr pcr NOUN njb-55 94 4     SPACE njb-55 94 5 were be AUX njb-55 94 6     SPACE njb-55 94 7 carried carry VERB njb-55 94 8     SPACE njb-55 94 9 out out ADP njb-55 94 10     SPACE njb-55 94 11 for for ADP njb-55 94 12     SPACE njb-55 94 13 confirmation confirmation NOUN njb-55 94 14     SPACE njb-55 94 15 of of ADP njb-55 94 16     SPACE njb-55 94 17 the the DET njb-55 94 18     SPACE njb-55 94 19 recombinants recombinant NOUN njb-55 94 20     SPACE njb-55 94 21 using use VERB njb-55 94 22     SPACE njb-55 94 23 primer primer NOUN njb-55 94 24     SPACE njb-55 94 25 p28/29 p28/29 PROPN njb-55 94 26     SPACE njb-55 94 27 and and CCONJ njb-55 94 28     SPACE njb-55 94 29 p30/31 p30/31 PROPN njb-55 94 30     SPACE njb-55 94 31 under under ADP njb-55 94 32     SPACE njb-55 94 33 the the DET njb-55 94 34     SPACE njb-55 94 35 standardized standardized ADJ njb-55 94 36     SPACE njb-55 94 37 conditions condition NOUN njb-55 94 38     SPACE njb-55 94 39 as as ADP njb-55 94 40     SPACE njb-55 94 41 described described AUX njb-55 94 42     SPACE njb-55 94 43 previously previously ADV njb-55 94 44     SPACE njb-55 94 45 using use VERB njb-55 94 46     SPACE njb-55 94 47 the the DET njb-55 94 48     SPACE njb-55 94 49 plasmid plasmid PROPN njb-55 94 50     SPACE njb-55 94 51 dna dna NOUN njb-55 94 52     SPACE njb-55 94 53 as as ADP njb-55 94 54     SPACE njb-55 94 55 template template NOUN njb-55 94 56 . . PUNCT njb-55 94 57     SPACE njb-55 95 1 it it PRON njb-55 95 2     SPACE njb-55 95 3 was be AUX njb-55 95 4     SPACE njb-55 95 5 performed perform VERB njb-55 95 6     SPACE njb-55 95 7 in in ADP njb-55 95 8     SPACE njb-55 95 9 a a DET njb-55 95 10     SPACE njb-55 95 11 25 25 NUM njb-55 95 12     SPACE njb-55 95 13 ml ml PROPN njb-55 95 14     SPACE njb-55 95 15 reaction reaction NOUN njb-55 95 16     SPACE njb-55 95 17 volume volume NOUN njb-55 95 18     SPACE njb-55 95 19 containing contain VERB njb-55 95 20     SPACE njb-55 95 21 10x 10x NOUN njb-55 95 22     SPACE njb-55 95 23 buffer buffer NOUN njb-55 95 24     SPACE njb-55 95 25 ( ( PUNCT njb-55 95 26 100 100 NUM njb-55 95 27 mm mm PROPN njb-55 95 28     SPACE njb-55 95 29 tris tris PROPN njb-55 95 30     SPACE njb-55 95 31 hcl hcl PROPN njb-55 95 32 , , PUNCT njb-55 95 33     SPACE njb-55 95 34 50 50 NUM njb-55 95 35 mm mm NOUN njb-55 95 36     SPACE njb-55 95 37 kcl kcl PROPN njb-55 95 38 , , PUNCT njb-55 95 39     SPACE njb-55 95 40 0.8%nonidet 0.8%nonidet NUM njb-55 95 41     SPACE njb-55 95 42 p40 p40 NOUN njb-55 95 43 ) ) PUNCT njb-55 95 44 , , PUNCT njb-55 95 45     SPACE njb-55 95 46 1.5 1.5 NUM njb-55 95 47 mm mm PROPN njb-55 95 48     SPACE njb-55 95 49 mgcl2 mgcl2 PROPN njb-55 95 50 , , PUNCT njb-55 95 51     SPACE njb-55 95 52 2.5 2.5 NUM njb-55 95 53 mm mm NOUN njb-55 95 54     SPACE njb-55 95 55 of of ADP njb-55 95 56     SPACE njb-55 95 57 each each DET njb-55 95 58     SPACE njb-55 95 59 dntps dntps NOUN njb-55 95 60 , , PUNCT njb-55 95 61     SPACE njb-55 95 62 2u 2u ADJ njb-55 95 63     SPACE njb-55 95 64 of of ADP njb-55 95 65     SPACE njb-55 95 66 taq taq PROPN njb-55 95 67     SPACE njb-55 95 68 polymerase polymerase NOUN njb-55 95 69 , , PUNCT njb-55 95 70     SPACE njb-55 95 71 25pmoles 25pmoles NUM njb-55 95 72     SPACE njb-55 95 73 each each DET njb-55 95 74     SPACE njb-55 95 75 primer primer NOUN njb-55 95 76     SPACE njb-55 95 77 and and CCONJ njb-55 95 78     SPACE njb-55 95 79 1ml 1ml NOUN njb-55 95 80     SPACE njb-55 95 81 of of ADP njb-55 95 82     SPACE njb-55 95 83 template template NOUN njb-55 95 84     SPACE njb-55 95 85 ( ( PUNCT njb-55 95 86 1:10 1:10 NUM njb-55 95 87     SPACE njb-55 95 88 diluted dilute VERB njb-55 95 89 ) ) PUNCT njb-55 95 90 . . PUNCT njb-55 95 91             SPACE njb-55 96 1 5ml 5ml PROPN njb-55 96 2     SPACE njb-55 96 3 aliquot aliquot PROPN njb-55 96 4     SPACE njb-55 96 5 of of ADP njb-55 96 6     SPACE njb-55 96 7 pcr pcr NOUN njb-55 96 8     SPACE njb-55 96 9 products product NOUN njb-55 96 10     SPACE njb-55 96 11 mixed mix VERB njb-55 96 12     SPACE njb-55 96 13 with with ADP njb-55 96 14     SPACE njb-55 96 15 1µl 1µl NOUN njb-55 96 16     SPACE njb-55 96 17 of of ADP njb-55 96 18     SPACE njb-55 96 19 6x 6x PROPN njb-55 96 20     SPACE njb-55 96 21 loading loading NOUN njb-55 96 22     SPACE njb-55 96 23 dye dye NOUN njb-55 96 24     SPACE njb-55 96 25 ( ( PUNCT njb-55 96 26 fermentas fermenta NOUN njb-55 96 27 ) ) PUNCT njb-55 96 28     SPACE njb-55 96 29 and and CCONJ njb-55 96 30     SPACE njb-55 96 31 loaded load VERB njb-55 96 32     SPACE njb-55 96 33 on on ADP njb-55 96 34     SPACE njb-55 96 35 to to ADP njb-55 96 36     SPACE njb-55 96 37 the the DET njb-55 96 38     SPACE njb-55 96 39 wells well NOUN njb-55 96 40     SPACE njb-55 96 41 of of ADP njb-55 96 42     SPACE njb-55 96 43 2.5 2.5 NUM njb-55 96 44 % % NOUN njb-55 96 45     SPACE njb-55 96 46 agarose agarose NOUN njb-55 96 47     SPACE njb-55 96 48 gel gel NOUN njb-55 96 49 . . PUNCT njb-55 96 50     SPACE njb-55 97 1 it it PRON njb-55 97 2     SPACE njb-55 97 3 was be AUX njb-55 97 4     SPACE njb-55 97 5 run run VERB njb-55 97 6     SPACE njb-55 97 7 at at ADP njb-55 97 8     SPACE njb-55 97 9 80v 80v NUM njb-55 97 10     SPACE njb-55 97 11 for for ADP njb-55 97 12     SPACE njb-55 97 13 about about ADV njb-55 97 14     SPACE njb-55 97 15 45 45 NUM njb-55 97 16     SPACE njb-55 97 17 min min PROPN njb-55 97 18 . . PROPN njb-55 97 19     SPACE njb-55 97 20 dna dna PROPN njb-55 97 21     SPACE njb-55 97 22 bands band NOUN njb-55 97 23     SPACE njb-55 97 24 were be AUX njb-55 97 25     SPACE njb-55 97 26 visualized visualize VERB njb-55 97 27     SPACE njb-55 97 28 under under ADP njb-55 97 29     SPACE njb-55 97 30 uv‐transilluminator uv‐transilluminator NOUN njb-55 97 31     SPACE njb-55 97 32 and and CCONJ njb-55 97 33     SPACE njb-55 97 34 documented document VERB njb-55 97 35     SPACE njb-55 97 36 using use VERB njb-55 97 37     SPACE njb-55 97 38 biorad biorad NOUN njb-55 97 39     SPACE njb-55 97 40 gel gel NOUN njb-55 97 41     SPACE njb-55 97 42 documentation documentation NOUN njb-55 97 43     SPACE njb-55 97 44 system system NOUN njb-55 97 45     SPACE njb-55 97 46 with with ADP njb-55 97 47     SPACE njb-55 97 48 quantity quantity NOUN njb-55 97 49     SPACE njb-55 97 50 one one NUM njb-55 97 51     SPACE njb-55 97 52 software software NOUN njb-55 97 53     SPACE njb-55 97 54 ( ( PUNCT njb-55 98 1 fig fig NOUN njb-55 98 2 . . PUNCT njb-55 98 3     SPACE njb-55 98 4 6a 6a NOUN njb-55 98 5     SPACE njb-55 98 6 and and CCONJ njb-55 98 7     SPACE njb-55 98 8 6b 6b NUM njb-55 98 9 ) ) PUNCT njb-55 98 10 . . PUNCT njb-55 98 11       SPACE njb-55 99 1 characterization characterization NOUN njb-55 99 2     SPACE njb-55 99 3 of of ADP njb-55 99 4     SPACE njb-55 99 5 amplicons amplicon NOUN njb-55 99 6 . . PUNCT njb-55 99 7     SPACE njb-55 100 1 the the DET njb-55 100 2     SPACE njb-55 100 3 pcr pcr NOUN njb-55 100 4     SPACE njb-55 100 5 product product NOUN njb-55 100 6     SPACE njb-55 100 7 of of ADP njb-55 100 8     SPACE njb-55 100 9 primer primer NOUN njb-55 100 10     SPACE njb-55 100 11 p28/29 p28/29 PROPN njb-55 100 12     SPACE njb-55 100 13 and and CCONJ njb-55 100 14     SPACE njb-55 100 15 primer primer NOUN njb-55 100 16     SPACE njb-55 100 17 p30/31 p30/31 PROPN njb-55 100 18     SPACE njb-55 100 19 were be AUX njb-55 100 20     SPACE njb-55 100 21 subjected subject VERB njb-55 100 22     SPACE njb-55 100 23 to to PART njb-55 100 24     SPACE njb-55 100 25 re re VERB njb-55 100 26     SPACE njb-55 100 27 digestion digestion NOUN njb-55 100 28 . . PUNCT njb-55 100 29     SPACE njb-55 101 1 the the DET njb-55 101 2     SPACE njb-55 101 3 enzymes enzyme NOUN njb-55 101 4     SPACE njb-55 101 5 rsai(gtˉac rsai(gtˉac NOUN njb-55 101 6 ) ) PUNCT njb-55 101 7     SPACE njb-55 101 8 and and CCONJ njb-55 101 9     SPACE njb-55 101 10 hindiii hindiii PROPN njb-55 101 11 ( ( PUNCT njb-55 101 12 aˉagctt aˉagctt PROPN njb-55 101 13 ) ) PUNCT njb-55 101 14     SPACE njb-55 101 15 were be AUX njb-55 101 16     SPACE njb-55 101 17 used use VERB njb-55 101 18     SPACE njb-55 101 19 to to PART njb-55 101 20     SPACE njb-55 101 21 characterize characterize VERB njb-55 101 22     SPACE njb-55 101 23 pcr pcr DET njb-55 101 24     SPACE njb-55 101 25 product product NOUN njb-55 101 26     SPACE njb-55 101 27 of of ADP njb-55 101 28     SPACE njb-55 101 29 p28/29 p28/29 PROPN njb-55 101 30     SPACE njb-55 101 31 ( ( PUNCT njb-55 101 32 fig fig NOUN njb-55 101 33 . . PUNCT njb-55 101 34     SPACE njb-55 101 35 2a 2a NUM njb-55 101 36 ) ) PUNCT njb-55 101 37     SPACE njb-55 101 38 and and CCONJ njb-55 101 39     SPACE njb-55 101 40 enzymes enzyme NOUN njb-55 101 41     SPACE njb-55 101 42 clai(atˉcgat clai(atˉcgat PROPN njb-55 101 43 ) ) PUNCT njb-55 101 44 , , PUNCT njb-55 101 45     SPACE njb-55 101 46 taqi taqi ADJ njb-55 101 47 ( ( PUNCT njb-55 101 48 tˉcga tˉcga NOUN njb-55 101 49 ) ) PUNCT njb-55 101 50     SPACE njb-55 101 51 and and CCONJ njb-55 101 52     SPACE njb-55 101 53 rsai(gtˉac rsai(gtˉac NOUN njb-55 101 54 ) ) PUNCT njb-55 101 55     SPACE njb-55 101 56 were be AUX njb-55 101 57     SPACE njb-55 101 58 used use VERB njb-55 101 59     SPACE njb-55 101 60 to to PART njb-55 101 61     SPACE njb-55 101 62 characterize characterize VERB njb-55 101 63     SPACE njb-55 101 64 pcr pcr DET njb-55 101 65     SPACE njb-55 101 66 product product NOUN njb-55 101 67     SPACE njb-55 101 68 of of ADP njb-55 101 69     SPACE njb-55 101 70 p30/31 p30/31 NUM njb-55 101 71     SPACE njb-55 101 72 ( ( PUNCT njb-55 101 73 fig fig NOUN njb-55 101 74 . . PUNCT njb-55 101 75     SPACE njb-55 101 76 2b 2b NUM njb-55 101 77 ) ) PUNCT njb-55 101 78 . . PUNCT njb-55 101 79         SPACE njb-55 102 1 the the DET njb-55 102 2     SPACE njb-55 102 3 reactions reaction NOUN njb-55 102 4     SPACE njb-55 102 5 were be AUX njb-55 102 6     SPACE njb-55 102 7 set set VERB njb-55 102 8     SPACE njb-55 102 9 as as ADV njb-55 102 10     SPACE njb-55 102 11 under under ADV njb-55 102 12     SPACE njb-55 102 13 and and CCONJ njb-55 102 14     SPACE njb-55 102 15 incubated incubate VERB njb-55 102 16     SPACE njb-55 102 17 at at ADP njb-55 102 18     SPACE njb-55 102 19 37 37 NUM njb-55 102 20 ° ° NOUN njb-55 102 21 c c NOUN njb-55 102 22     SPACE njb-55 102 23 in in ADP njb-55 102 24     SPACE njb-55 102 25 a a DET njb-55 102 26     SPACE njb-55 102 27 water water NOUN njb-55 102 28     SPACE njb-55 102 29 bath bath NOUN njb-55 102 30     SPACE njb-55 102 31 for for ADP njb-55 102 32     SPACE njb-55 102 33 4h 4h NUM njb-55 102 34 . . PUNCT njb-55 102 35     SPACE njb-55 102 36 restriction restriction NOUN njb-55 102 37     SPACE njb-55 102 38 enzymes enzyme NOUN njb-55 102 39     SPACE njb-55 102 40 and and CCONJ njb-55 102 41     SPACE njb-55 102 42 its its PRON njb-55 102 43     SPACE njb-55 102 44 corresponding correspond VERB njb-55 102 45     SPACE njb-55 102 46 buffer buffer NOUN njb-55 102 47     SPACE njb-55 102 48 were be AUX njb-55 102 49     SPACE njb-55 102 50 procured procure VERB njb-55 102 51     SPACE njb-55 102 52 from from ADP njb-55 102 53     SPACE njb-55 102 54 fermentas fermenta NOUN njb-55 102 55 . . PUNCT njb-55 102 56         SPACE njb-55 103 1 clai clai PROPN njb-55 103 2     SPACE njb-55 103 3 and and CCONJ njb-55 103 4     SPACE njb-55 103 5 its its PRON njb-55 103 6     SPACE njb-55 103 7 corresponding correspond VERB njb-55 103 8     SPACE njb-55 103 9 buffer buffer NOUN njb-55 103 10     SPACE njb-55 103 11 were be AUX njb-55 103 12     SPACE njb-55 103 13 procured procure VERB njb-55 103 14     SPACE njb-55 103 15 from from ADP njb-55 103 16     SPACE njb-55 103 17 promega promega PROPN njb-55 103 18 . . PUNCT njb-55 103 19       SPACE njb-55 104 1 details detail NOUN njb-55 104 2     SPACE njb-55 104 3 of of ADP njb-55 104 4     SPACE njb-55 104 5 the the DET njb-55 104 6     SPACE njb-55 104 7 reaction reaction NOUN njb-55 104 8     SPACE njb-55 104 9 mixture mixture NOUN njb-55 104 10     SPACE njb-55 104 11 are be AUX njb-55 104 12     SPACE njb-55 104 13 summarized summarize VERB njb-55 104 14     SPACE njb-55 104 15 in in ADP njb-55 104 16     SPACE njb-55 104 17 table table NOUN njb-55 104 18     SPACE njb-55 104 19 3 3 NUM njb-55 104 20 . . PUNCT njb-55 104 21       SPACE njb-55 104 22 a a DET njb-55 104 23     SPACE njb-55 104 24 15 15 NUM njb-55 104 25     SPACE njb-55 104 26 ml ml PROPN njb-55 104 27     SPACE njb-55 104 28 aliquot aliquot NOUN njb-55 104 29     SPACE njb-55 104 30 of of ADP njb-55 104 31     SPACE njb-55 104 32 each each DET njb-55 104 33     SPACE njb-55 104 34 digested digest VERB njb-55 104 35     SPACE njb-55 104 36 products product NOUN njb-55 104 37     SPACE njb-55 104 38 were be AUX njb-55 104 39     SPACE njb-55 104 40 mixed mix VERB njb-55 104 41     SPACE njb-55 104 42 with with ADP njb-55 104 43     SPACE njb-55 104 44 3µl 3µl PROPN njb-55 104 45     SPACE njb-55 104 46 of of ADP njb-55 104 47     SPACE njb-55 104 48 6x 6x ADJ njb-55 104 49     SPACE njb-55 104 50 loading loading NOUN njb-55 104 51     SPACE njb-55 104 52 dye dye NOUN njb-55 104 53     SPACE njb-55 104 54 ( ( PUNCT njb-55 104 55 fermentas fermenta NOUN njb-55 104 56 ) ) PUNCT njb-55 104 57     SPACE njb-55 104 58 and and CCONJ njb-55 104 59     SPACE njb-55 104 60 loaded load VERB njb-55 104 61     SPACE njb-55 104 62 on on ADV njb-55 104 63     SPACE njb-55 104 64 to to ADP njb-55 104 65     SPACE njb-55 104 66 the the DET njb-55 104 67     SPACE njb-55 104 68 wells well NOUN njb-55 104 69     SPACE njb-55 104 70 of of ADP njb-55 104 71     SPACE njb-55 104 72 2 2 NUM njb-55 104 73 % % NOUN njb-55 104 74     SPACE njb-55 104 75 agarose agarose NOUN njb-55 104 76     SPACE njb-55 104 77 gel gel NOUN njb-55 104 78     SPACE njb-55 104 79 in in ADP njb-55 104 80     SPACE njb-55 104 81 tae tae NOUN njb-55 104 82     SPACE njb-55 104 83 buffer buffer NOUN njb-55 104 84 . . PUNCT njb-55 104 85     SPACE njb-55 105 1 it it PRON njb-55 105 2     SPACE njb-55 105 3 was be AUX njb-55 105 4     SPACE njb-55 105 5 run run VERB njb-55 105 6     SPACE njb-55 105 7 at at ADP njb-55 105 8     SPACE njb-55 105 9 80v 80v NUM njb-55 105 10     SPACE njb-55 105 11 for for ADP njb-55 105 12     SPACE njb-55 105 13 about about ADV njb-55 105 14     SPACE njb-55 105 15 45 45 NUM njb-55 105 16     SPACE njb-55 105 17 min min PROPN njb-55 105 18 . . PROPN njb-55 105 19     SPACE njb-55 105 20 dna dna PROPN njb-55 105 21     SPACE njb-55 105 22 bands band NOUN njb-55 105 23     SPACE njb-55 105 24 were be AUX njb-55 105 25     SPACE njb-55 105 26 visualized visualize VERB njb-55 105 27     SPACE njb-55 105 28 under under ADP njb-55 105 29     SPACE njb-55 105 30 uv‐transi uv‐transi PROPN njb-55 105 31     SPACE njb-55 105 32 lluminator lluminator PROPN njb-55 105 33     SPACE njb-55 105 34 and and CCONJ njb-55 105 35     SPACE njb-55 105 36 documented document VERB njb-55 105 37     SPACE njb-55 105 38 using use VERB njb-55 105 39     SPACE njb-55 105 40 gel gel NOUN njb-55 105 41     SPACE njb-55 105 42 documentation documentation NOUN njb-55 105 43     SPACE njb-55 105 44 system system NOUN njb-55 105 45     SPACE njb-55 105 46 with with ADP njb-55 105 47     SPACE njb-55 105 48 quantity quantity NOUN njb-55 105 49     SPACE njb-55 105 50 one one NUM njb-55 105 51     SPACE njb-55 105 52 software software NOUN njb-55 105 53 ( ( PUNCT njb-55 105 54 biorad biorad NOUN njb-55 105 55 , , PUNCT njb-55 105 56     SPACE njb-55 105 57 u.s.a u.s.a NOUN njb-55 105 58 ) ) PUNCT njb-55 105 59     SPACE njb-55 105 60 ( ( PUNCT njb-55 105 61 fig fig NOUN njb-55 105 62 . . PUNCT njb-55 105 63     SPACE njb-55 106 1 2a 2a NUM njb-55 106 2     SPACE njb-55 106 3 and and CCONJ njb-55 106 4     SPACE njb-55 106 5 2b 2b NUM njb-55 106 6 ) ) PUNCT njb-55 106 7 . . PUNCT njb-55 106 8     SPACE njb-55 107 1 specificity specificity NOUN njb-55 107 2     SPACE njb-55 107 3 the the DET njb-55 107 4     SPACE njb-55 107 5 pcr pcr PROPN njb-55 107 6     SPACE njb-55 107 7 assay assay NOUN njb-55 107 8 . . PUNCT njb-55 107 9     SPACE njb-55 108 1 the the DET njb-55 108 2     SPACE njb-55 108 3 specificity specificity NOUN njb-55 108 4     SPACE njb-55 108 5 of of ADP njb-55 108 6     SPACE njb-55 108 7 both both DET njb-55 108 8     SPACE njb-55 108 9 the the DET njb-55 108 10     SPACE njb-55 108 11 assay assay NOUN njb-55 108 12     SPACE njb-55 108 13 developed develop VERB njb-55 108 14     SPACE njb-55 108 15 was be AUX njb-55 108 16     SPACE njb-55 108 17 tested test VERB njb-55 108 18     SPACE njb-55 108 19 by by ADP njb-55 108 20     SPACE njb-55 108 21 taking take VERB njb-55 108 22     SPACE njb-55 108 23 10‐20ng 10‐20ng NUM njb-55 108 24     SPACE njb-55 108 25 of of ADP njb-55 108 26     SPACE njb-55 108 27 each each DET njb-55 108 28     SPACE njb-55 108 29 template template NOUN njb-55 108 30     SPACE njb-55 108 31 dna dna NOUN njb-55 108 32     SPACE njb-55 108 33 isolated isolate VERB njb-55 108 34     SPACE njb-55 108 35 from from ADP njb-55 108 36     SPACE njb-55 108 37 the the DET njb-55 108 38     SPACE njb-55 108 39 different different ADJ njb-55 108 40     SPACE njb-55 108 41 serovars serovar NOUN njb-55 108 42     SPACE njb-55 108 43 and and CCONJ njb-55 108 44     SPACE njb-55 108 45 the the DET njb-55 108 46     SPACE njb-55 108 47 pcr pcr NOUN njb-55 108 48     SPACE njb-55 108 49 was be AUX njb-55 108 50     SPACE njb-55 108 51 carried carry VERB njb-55 108 52     SPACE njb-55 108 53 out out ADP njb-55 108 54     SPACE njb-55 108 55 under under ADP njb-55 108 56     SPACE njb-55 108 57 the the DET njb-55 108 58     SPACE njb-55 108 59 standardized standardize VERB njb-55 108 60     SPACE njb-55 108 61 conditions condition NOUN njb-55 108 62     SPACE njb-55 108 63 as as SCONJ njb-55 108 64     SPACE njb-55 108 65 shown show VERB njb-55 108 66     SPACE njb-55 108 67 in in ADP njb-55 108 68     SPACE njb-55 108 69 table table NOUN njb-55 108 70     SPACE njb-55 108 71 ( ( PUNCT njb-55 108 72 table table NOUN njb-55 108 73     SPACE njb-55 108 74 4 4 NUM njb-55 108 75 ) ) PUNCT njb-55 108 76 . . PUNCT njb-55 108 77     SPACE njb-55 109 1 a a DET njb-55 109 2     SPACE njb-55 109 3 10ml 10ml NOUN njb-55 109 4     SPACE njb-55 109 5 aliquot aliquot NOUN njb-55 109 6     SPACE njb-55 109 7 of of ADP njb-55 109 8     SPACE njb-55 109 9 each each DET njb-55 109 10     SPACE njb-55 109 11 pcr pcr NOUN njb-55 109 12     SPACE njb-55 109 13 product product NOUN njb-55 109 14     SPACE njb-55 109 15 mixed mix VERB njb-55 109 16     SPACE njb-55 109 17 with with ADP njb-55 109 18     SPACE njb-55 109 19 2ml 2ml NOUN njb-55 109 20     SPACE njb-55 109 21 of of ADP njb-55 109 22     SPACE njb-55 109 23 6x 6x ADJ njb-55 109 24     SPACE njb-55 109 25 loading loading NOUN njb-55 109 26     SPACE njb-55 109 27 dye dye NOUN njb-55 109 28     SPACE njb-55 109 29 ( ( PUNCT njb-55 109 30 fermentas fermenta NOUN njb-55 109 31 ) ) PUNCT njb-55 109 32     SPACE njb-55 109 33 was be AUX njb-55 109 34     SPACE njb-55 109 35 run run VERB njb-55 109 36     SPACE njb-55 109 37 on on ADP njb-55 109 38     SPACE njb-55 109 39 a a DET njb-55 109 40     SPACE njb-55 109 41 2.5 2.5 NUM njb-55 109 42 % % NOUN njb-55 109 43     SPACE njb-55 109 44 agarose agarose NOUN njb-55 109 45     SPACE njb-55 109 46 electrophoretic electrophoretic NOUN njb-55 109 47     SPACE njb-55 109 48 gel gel NOUN njb-55 109 49     SPACE njb-55 109 50 in in ADP njb-55 109 51     SPACE njb-55 109 52 tae tae NOUN njb-55 109 53     SPACE njb-55 109 54 buffer buffer NOUN njb-55 109 55     SPACE njb-55 109 56 and and CCONJ njb-55 109 57     SPACE njb-55 109 58 dna dna PROPN njb-55 109 59     SPACE njb-55 109 60 bands band NOUN njb-55 109 61     SPACE njb-55 109 62 were be AUX njb-55 109 63     SPACE njb-55 109 64 visualized visualize VERB njb-55 109 65     SPACE njb-55 109 66 under under ADP njb-55 109 67     SPACE njb-55 109 68 uv‐transilluminator uv‐transilluminator NOUN njb-55 109 69     SPACE njb-55 109 70 and and CCONJ njb-55 109 71     SPACE njb-55 109 72 documented document VERB njb-55 109 73     SPACE njb-55 109 74 using use VERB njb-55 109 75     SPACE njb-55 109 76 gel gel NOUN njb-55 109 77     SPACE njb-55 109 78 documentation documentation NOUN njb-55 109 79     SPACE njb-55 109 80 system system NOUN njb-55 109 81     SPACE njb-55 109 82 with with ADP njb-55 109 83     SPACE njb-55 109 84 quantity quantity NOUN njb-55 109 85     SPACE njb-55 109 86 one one NUM njb-55 109 87     SPACE njb-55 109 88 software software NOUN njb-55 109 89     SPACE njb-55 109 90 ( ( PUNCT njb-55 109 91 biorad biorad NOUN njb-55 109 92 , , PUNCT njb-55 109 93     SPACE njb-55 109 94 u.s.a u.s.a NOUN njb-55 109 95 ) ) PUNCT njb-55 109 96     SPACE njb-55 109 97 ( ( PUNCT njb-55 109 98 fig fig NOUN njb-55 109 99 . . PUNCT njb-55 109 100     SPACE njb-55 110 1 3a 3a NUM njb-55 110 2     SPACE njb-55 110 3 and and CCONJ njb-55 110 4     SPACE njb-55 110 5 3b 3b NUM njb-55 110 6 ) ) PUNCT njb-55 110 7 . . PUNCT njb-55 110 8     SPACE njb-55 110 9 transformation transformation NOUN njb-55 110 10 . . PUNCT njb-55 110 11     SPACE njb-55 111 1 ligation ligation NOUN njb-55 111 2     SPACE njb-55 111 3 mixture mixture NOUN njb-55 111 4     SPACE njb-55 111 5 was be AUX njb-55 111 6     SPACE njb-55 111 7 prepared prepare VERB njb-55 111 8     SPACE njb-55 111 9 by by ADP njb-55 111 10     SPACE njb-55 111 11 adding add VERB njb-55 111 12     SPACE njb-55 111 13 1ml 1ml NOUN njb-55 111 14     SPACE njb-55 111 15 of of ADP njb-55 111 16     SPACE njb-55 111 17 pgemt pgemt NOUN njb-55 111 18     SPACE njb-55 111 19 easy easy ADJ njb-55 111 20     SPACE njb-55 111 21 ( ( PUNCT njb-55 111 22 50ng 50ng NOUN njb-55 111 23 ) ) PUNCT njb-55 111 24     SPACE njb-55 111 25 vector vector NOUN njb-55 111 26     SPACE njb-55 111 27 at at ADP njb-55 111 28     SPACE njb-55 111 29 the the DET njb-55 111 30     SPACE njb-55 111 31 rate rate NOUN njb-55 111 32     SPACE njb-55 111 33 of of ADP njb-55 111 34     SPACE njb-55 111 35 and and CCONJ njb-55 111 36     SPACE njb-55 111 37 1ml 1ml NOUN njb-55 111 38     SPACE njb-55 111 39 of of ADP njb-55 111 40     SPACE njb-55 111 41 t4 t4 PROPN njb-55 111 42     SPACE njb-55 111 43 dna dna PROPN njb-55 111 44     SPACE njb-55 111 45 ligase ligase PROPN njb-55 111 46     SPACE njb-55 111 47 ( ( PUNCT njb-55 111 48 3u 3u NUM njb-55 111 49 / / SYM njb-55 111 50 ml ml PROPN njb-55 111 51 ) ) PUNCT njb-55 111 52     SPACE njb-55 111 53 to to ADP njb-55 111 54     SPACE njb-55 111 55 5ml 5ml PROPN njb-55 111 56     SPACE njb-55 111 57 of of ADP njb-55 111 58     SPACE njb-55 111 59 2x 2x NOUN njb-55 111 60     SPACE njb-55 111 61 buffer buffer NOUN njb-55 111 62 . . PUNCT njb-55 111 63     SPACE njb-55 112 1 5ml 5ml PROPN njb-55 112 2     SPACE njb-55 112 3 of of ADP njb-55 112 4     SPACE njb-55 112 5 dna dna NOUN njb-55 112 6     SPACE njb-55 112 7 ( ( PUNCT njb-55 112 8 50ng 50ng NUM njb-55 112 9 / / SYM njb-55 112 10 ml ml PROPN njb-55 112 11 ) ) PUNCT njb-55 112 12     SPACE njb-55 112 13 was be AUX njb-55 112 14     SPACE njb-55 112 15 added add VERB njb-55 112 16     SPACE njb-55 112 17 to to ADP njb-55 112 18     SPACE njb-55 112 19 this this DET njb-55 112 20     SPACE njb-55 112 21 mixture mixture NOUN njb-55 112 22     SPACE njb-55 112 23 for for ADP njb-55 112 24     SPACE njb-55 112 25 ligation ligation NOUN njb-55 112 26 . . PUNCT njb-55 112 27     SPACE njb-55 113 1 ligation ligation NOUN njb-55 113 2     SPACE njb-55 113 3 was be AUX njb-55 113 4     SPACE njb-55 113 5 done do VERB njb-55 113 6     SPACE njb-55 113 7 at at ADP njb-55 113 8     SPACE njb-55 113 9 4 4 NUM njb-55 113 10 ° ° NOUN njb-55 113 11 c c NOUN njb-55 113 12     SPACE njb-55 113 13 for for ADP njb-55 113 14     SPACE njb-55 113 15 overnight overnight ADV njb-55 113 16 . . PUNCT njb-55 113 17     SPACE njb-55 114 1 ligation ligation NOUN njb-55 114 2     SPACE njb-55 114 3 mixture mixture NOUN njb-55 114 4     SPACE njb-55 114 5 was be AUX njb-55 114 6     SPACE njb-55 114 7 added add VERB njb-55 114 8     SPACE njb-55 114 9 to to ADP njb-55 114 10     SPACE njb-55 114 11 ice ice NOUN njb-55 114 12     SPACE njb-55 114 13 cold cold ADJ njb-55 114 14     SPACE njb-55 114 15 200ml 200ml NOUN njb-55 114 16     SPACE njb-55 114 17 of of ADP njb-55 114 18     SPACE njb-55 114 19 competent competent ADJ njb-55 114 20     SPACE njb-55 114 21 cells cell NOUN njb-55 114 22     SPACE njb-55 114 23 and and CCONJ njb-55 114 24     SPACE njb-55 114 25 tapped tap VERB njb-55 114 26     SPACE njb-55 114 27 gently gently ADV njb-55 114 28 , , PUNCT njb-55 114 29     SPACE njb-55 114 30 it it PRON njb-55 114 31     SPACE njb-55 114 32 was be AUX njb-55 114 33     SPACE njb-55 114 34 incubated incubate VERB njb-55 114 35     SPACE njb-55 114 36 on on ADP njb-55 114 37     SPACE njb-55 114 38 ice ice NOUN njb-55 114 39     SPACE njb-55 114 40 for for ADP njb-55 114 41     SPACE njb-55 114 42 30 30 NUM njb-55 114 43     SPACE njb-55 114 44 min min PROPN njb-55 114 45 . . PROPN njb-55 114 46 , , PUNCT njb-55 114 47     SPACE njb-55 114 48 then then ADV njb-55 114 49     SPACE njb-55 114 50 heat heat NOUN njb-55 114 51     SPACE njb-55 114 52 shock shock NOUN njb-55 114 53     SPACE njb-55 114 54 was be AUX njb-55 114 55     SPACE njb-55 114 56 given give VERB njb-55 114 57     SPACE njb-55 114 58 at at ADP njb-55 114 59     SPACE njb-55 114 60 42 42 NUM njb-55 114 61 ° ° NOUN njb-55 114 62 c c NOUN njb-55 114 63     SPACE njb-55 114 64 for for ADP njb-55 114 65     SPACE njb-55 114 66 1 1 NUM njb-55 114 67     SPACE njb-55 114 68 reaction reaction NOUN njb-55 114 69     SPACE njb-55 114 70 mixture mixture NOUN njb-55 114 71     SPACE njb-55 114 72 lipl21 lipl21 PROPN njb-55 114 73     SPACE njb-55 114 74 lipl41 lipl41 PROPN njb-55 114 75     SPACE njb-55 114 76 rsai rsai PROPN njb-55 114 77     SPACE njb-55 114 78 hindiii hindiii PROPN njb-55 114 79     SPACE njb-55 114 80 clai clai PROPN njb-55 114 81     SPACE njb-55 114 82 taqi taqi VERB njb-55 114 83     SPACE njb-55 114 84 rsai rsai VERB njb-55 114 85     SPACE njb-55 114 86 buffer buffer NOUN njb-55 114 87     SPACE njb-55 114 88 enzyme enzyme NOUN njb-55 114 89     SPACE njb-55 114 90 dna dna PROPN njb-55 114 91     SPACE njb-55 114 92 water water NOUN njb-55 114 93     SPACE njb-55 114 94 bsa bsa NOUN njb-55 114 95     SPACE njb-55 114 96 2.0 2.0 NUM njb-55 114 97     SPACE njb-55 114 98 ml ml PROPN njb-55 114 99     SPACE njb-55 114 100 1.0 1.0 NUM njb-55 114 101     SPACE njb-55 114 102 ml ml PROPN njb-55 114 103     SPACE njb-55 114 104 8.0 8.0 NUM njb-55 114 105       SPACE njb-55 114 106 ml ml PROPN njb-55 114 107     SPACE njb-55 114 108 11.0 11.0 NUM njb-55 114 109     SPACE njb-55 114 110 ml ml PROPN njb-55 114 111     SPACE njb-55 114 112 ‐ ‐ AUX njb-55 114 113     SPACE njb-55 114 114 2.0 2.0 NUM njb-55 114 115     SPACE njb-55 114 116 ml ml PROPN njb-55 114 117     SPACE njb-55 114 118 1.0 1.0 NUM njb-55 114 119     SPACE njb-55 114 120 ml ml PROPN njb-55 114 121     SPACE njb-55 114 122 8.0 8.0 NUM njb-55 114 123     SPACE njb-55 114 124 ml ml PROPN njb-55 114 125     SPACE njb-55 114 126 11.0 11.0 NUM njb-55 114 127     SPACE njb-55 114 128 ml ml PROPN njb-55 114 129     SPACE njb-55 114 130 ‐‐‐‐‐‐ ‐‐‐‐‐‐ PROPN njb-55 114 131     SPACE njb-55 114 132 2.0ml 2.0ml NUM njb-55 114 133     SPACE njb-55 114 134 1.0ml 1.0ml NUM njb-55 114 135     SPACE njb-55 114 136 5.0ml 5.0ml NUM njb-55 114 137     SPACE njb-55 114 138 10.0ml 10.0ml NUM njb-55 114 139     SPACE njb-55 114 140 2.0ml 2.0ml NUM njb-55 114 141     SPACE njb-55 114 142 2.0ml 2.0ml NUM njb-55 114 143     SPACE njb-55 114 144 1.0ml 1.0ml NUM njb-55 114 145     SPACE njb-55 114 146 5.0ml 5.0ml NUM njb-55 114 147     SPACE njb-55 114 148 12.0ml 12.0ml NUM njb-55 114 149     SPACE njb-55 114 150 ‐ ‐ AUX njb-55 114 151     SPACE njb-55 114 152 2.0ml 2.0ml NUM njb-55 114 153     SPACE njb-55 114 154 1.0ml 1.0ml NUM njb-55 114 155     SPACE njb-55 114 156 5.0ml 5.0ml NUM njb-55 114 157     SPACE njb-55 114 158 12.0ml 12.0ml NUM njb-55 114 159     SPACE njb-55 114 160 ‐ ‐ AUX njb-55 114 161     SPACE njb-55 114 162 table table NOUN njb-55 114 163     SPACE njb-55 114 164 3 3 NUM njb-55 114 165 . . PUNCT njb-55 114 166     SPACE njb-55 115 1 details detail NOUN njb-55 115 2     SPACE njb-55 115 3 of of ADP njb-55 115 4     SPACE njb-55 115 5 the the DET njb-55 115 6     SPACE njb-55 115 7 restriction restriction NOUN njb-55 115 8     SPACE njb-55 115 9 enzyme enzyme NOUN njb-55 115 10     SPACE njb-55 115 11 digestion digestion NOUN njb-55 115 12     SPACE njb-55 115 13 reaction reaction NOUN njb-55 115 14     SPACE njb-55 115 15 of of ADP njb-55 115 16     SPACE njb-55 115 17 lipl21 lipl21 NOUN njb-55 115 18     SPACE njb-55 115 19 and and CCONJ njb-55 115 20     SPACE njb-55 115 21 lipl41 lipl41 PROPN njb-55 115 22     SPACE njb-55 115 23 nepal nepal ADJ njb-55 115 24     SPACE njb-55 115 25 journal journal NOUN njb-55 115 26     SPACE njb-55 115 27 of of ADP njb-55 115 28     SPACE njb-55 115 29 biotechnology biotechnology NOUN njb-55 115 30 . . PUNCT njb-55 115 31     SPACE njb-55 116 1 jan jan PROPN njb-55 116 2 . . PROPN njb-55 116 3     SPACE njb-55 116 4 2011 2011 NUM njb-55 116 5 , , PUNCT njb-55 116 6     SPACE njb-55 116 7 vol vol NOUN njb-55 116 8 . . PUNCT njb-55 116 9     SPACE njb-55 116 10 1 1 NUM njb-55 116 11 , , PUNCT njb-55 116 12     SPACE njb-55 116 13 no no INTJ njb-55 116 14 . . PUNCT njb-55 116 15     SPACE njb-55 117 1 1 1 NUM njb-55 117 2 : : PUNCT njb-55 117 3     SPACE njb-55 117 4 22‐30 22‐30 NUM njb-55 117 5     SPACE njb-55 117 6 26 26 NUM njb-55 117 7     SPACE njb-55 117 8 biotechnology biotechnology NOUN njb-55 117 9     SPACE njb-55 117 10 society society NOUN njb-55 117 11     SPACE njb-55 117 12 of of ADP njb-55 117 13     SPACE njb-55 117 14 nepal nepal NOUN njb-55 117 15     SPACE njb-55 117 16 ( ( PUNCT njb-55 117 17 bsn bsn PROPN njb-55 117 18 ) ) PUNCT njb-55 117 19 , , PUNCT njb-55 117 20     SPACE njb-55 117 21 all all DET njb-55 117 22     SPACE njb-55 117 23 rights right NOUN njb-55 117 24     SPACE njb-55 117 25 reserved reserve VERB njb-55 117 26       SPACE njb-55 117 27 out out ADP njb-55 117 28     SPACE njb-55 117 29 reactions reaction NOUN njb-55 117 30     SPACE njb-55 117 31 at at ADP njb-55 117 32     SPACE njb-55 117 33 various various ADJ njb-55 117 34     SPACE njb-55 117 35 annealing annealing NOUN njb-55 117 36     SPACE njb-55 117 37 temperatures temperature NOUN njb-55 117 38     SPACE njb-55 117 39 ranging range VERB njb-55 117 40     SPACE njb-55 117 41 from from ADP njb-55 117 42     SPACE njb-55 117 43 58 58 NUM njb-55 117 44 ° ° PUNCT njb-55 117 45 c c NOUN njb-55 117 46     SPACE njb-55 117 47 to to ADP njb-55 117 48     SPACE njb-55 117 49 68 68 NUM njb-55 117 50 ° ° NOUN njb-55 118 1 c c NOUN njb-55 118 2     SPACE njb-55 118 3 on on ADP njb-55 118 4     SPACE njb-55 118 5 l. l. NOUN njb-55 118 6     SPACE njb-55 118 7 icterohaemorrhagiae icterohaemorrhagiae NOUN njb-55 118 8     SPACE njb-55 118 9 serovar serovar NOUN njb-55 118 10 . . PUNCT njb-55 118 11     SPACE njb-55 119 1 this this PRON njb-55 119 2     SPACE njb-55 119 3 showed show VERB njb-55 119 4     SPACE njb-55 119 5 non‐specific non‐specific NOUN njb-55 119 6     SPACE njb-55 119 7 amplification amplification NOUN njb-55 119 8     SPACE njb-55 119 9 at at ADP njb-55 119 10     SPACE njb-55 119 11 58 58 NUM njb-55 119 12 ° ° NOUN njb-55 119 13 c c NOUN njb-55 119 14 , , PUNCT njb-55 119 15     SPACE njb-55 119 16 58.7 58.7 NUM njb-55 119 17 ° ° NOUN njb-55 119 18 c c NOUN njb-55 119 19 , , PUNCT njb-55 119 20     SPACE njb-55 119 21 60.8 60.8 NUM njb-55 119 22 ° ° NOUN njb-55 119 23 c c NOUN njb-55 119 24     SPACE njb-55 119 25 and and CCONJ njb-55 119 26     SPACE njb-55 119 27 63.5 63.5 NUM njb-55 119 28 ° ° NOUN njb-55 119 29 c c NOUN njb-55 119 30 . . PUNCT njb-55 119 31     SPACE njb-55 119 32 so so ADV njb-55 119 33     SPACE njb-55 119 34 pcr pcr PROPN njb-55 119 35     SPACE njb-55 119 36 reaction reaction NOUN njb-55 119 37     SPACE njb-55 119 38 using use VERB njb-55 119 39     SPACE njb-55 119 40 p30/31 p30/31 PROPN njb-55 119 41     SPACE njb-55 119 42 was be AUX njb-55 119 43     SPACE njb-55 119 44 carried carry VERB njb-55 119 45     SPACE njb-55 119 46 out out ADP njb-55 119 47     SPACE njb-55 119 48 on on ADP njb-55 119 49     SPACE njb-55 119 50 l.javanica l.javanica PROPN njb-55 119 51     SPACE njb-55 119 52 template template NOUN njb-55 119 53     SPACE njb-55 119 54 at at ADP njb-55 119 55     SPACE njb-55 119 56 two two NUM njb-55 119 57     SPACE njb-55 119 58 different different ADJ njb-55 119 59     SPACE njb-55 119 60 mgcl2 mgcl2 NOUN njb-55 119 61     SPACE njb-55 119 62 concentration concentration NOUN njb-55 119 63     SPACE njb-55 119 64 i.e. i.e. ADV njb-55 119 65     SPACE njb-55 119 66 ( ( PUNCT njb-55 119 67 0.5 0.5 NUM njb-55 119 68 , , PUNCT njb-55 119 69     SPACE njb-55 119 70 1.0µl 1.0µl NUM njb-55 119 71 ) ) PUNCT njb-55 119 72 . . PUNCT njb-55 119 73     SPACE njb-55 120 1 no no DET njb-55 120 2     SPACE njb-55 120 3 bands band NOUN njb-55 120 4     SPACE njb-55 120 5 could could AUX njb-55 120 6     SPACE njb-55 120 7 be be AUX njb-55 120 8     SPACE njb-55 120 9 detected detect VERB njb-55 120 10     SPACE njb-55 120 11 for for ADP njb-55 120 12     SPACE njb-55 120 13 pcr pcr NOUN njb-55 120 14     SPACE njb-55 120 15 amplification amplification NOUN njb-55 120 16     SPACE njb-55 120 17 at at ADP njb-55 120 18     SPACE njb-55 120 19 0.5 0.5 NUM njb-55 120 20     SPACE njb-55 120 21 µl µl PROPN njb-55 120 22     SPACE njb-55 120 23 mgcl2 mgcl2 NOUN njb-55 120 24     SPACE njb-55 120 25 but but CCONJ njb-55 120 26     SPACE njb-55 120 27 specific specific ADJ njb-55 120 28     SPACE njb-55 120 29 band band NOUN njb-55 120 30     SPACE njb-55 120 31 of of ADP njb-55 120 32     SPACE njb-55 120 33 430bp 430bp NOUN njb-55 120 34     SPACE njb-55 120 35 was be AUX njb-55 120 36     SPACE njb-55 120 37 observed observe VERB njb-55 120 38     SPACE njb-55 120 39 at at ADP njb-55 120 40     SPACE njb-55 120 41 1.0 1.0 NUM njb-55 120 42     SPACE njb-55 120 43 µl µl PROPN njb-55 120 44     SPACE njb-55 120 45 mgcl2 mgcl2 PROPN njb-55 120 46 . . PUNCT njb-55 120 47         SPACE njb-55 120 48 standardization standardization NOUN njb-55 120 49     SPACE njb-55 120 50 was be AUX njb-55 120 51     SPACE njb-55 120 52 done do VERB njb-55 120 53     SPACE njb-55 120 54 by by ADP njb-55 120 55     SPACE njb-55 120 56 carrying carry VERB njb-55 120 57     SPACE njb-55 120 58 out out ADP njb-55 120 59     SPACE njb-55 120 60 reactions reaction NOUN njb-55 120 61     SPACE njb-55 120 62 at at ADP njb-55 120 63     SPACE njb-55 120 64 various various ADJ njb-55 120 65     SPACE njb-55 120 66 annealing annealing NOUN njb-55 120 67     SPACE njb-55 120 68 temperatures temperature NOUN njb-55 120 69     SPACE njb-55 120 70 from from ADP njb-55 120 71     SPACE njb-55 120 72 55 55 NUM njb-55 120 73 ° ° NOUN njb-55 120 74     SPACE njb-55 120 75 to to ADP njb-55 120 76     SPACE njb-55 120 77 65 65 NUM njb-55 120 78 ° ° NOUN njb-55 120 79 c c NOUN njb-55 120 80     SPACE njb-55 120 81 for for ADP njb-55 120 82     SPACE njb-55 120 83 1 1 NUM njb-55 120 84     SPACE njb-55 120 85 min min PROPN njb-55 120 86 . . PROPN njb-55 120 87     SPACE njb-55 120 88 good good ADJ njb-55 120 89     SPACE njb-55 120 90 amplification amplification NOUN njb-55 120 91     SPACE njb-55 120 92 bands band NOUN njb-55 120 93     SPACE njb-55 120 94 were be AUX njb-55 120 95     SPACE njb-55 120 96 observed observe VERB njb-55 120 97     SPACE njb-55 120 98 for for ADP njb-55 120 99     SPACE njb-55 120 100 pcr pcr NOUN njb-55 120 101     SPACE njb-55 120 102 reactions reaction NOUN njb-55 120 103     SPACE njb-55 120 104 carried carry VERB njb-55 120 105     SPACE njb-55 120 106 out out ADP njb-55 120 107     SPACE njb-55 120 108 at at ADP njb-55 120 109     SPACE njb-55 120 110 annealing anneal VERB njb-55 120 111     SPACE njb-55 120 112 temperatures temperature NOUN njb-55 120 113     SPACE njb-55 120 114 60.5 60.5 NUM njb-55 120 115 ° ° NOUN njb-55 120 116 c c NOUN njb-55 120 117 , , PUNCT njb-55 120 118     SPACE njb-55 120 119 61.8 61.8 NUM njb-55 120 120 ° ° NOUN njb-55 120 121 c c NOUN njb-55 120 122 , , PUNCT njb-55 120 123     SPACE njb-55 120 124 63.1 63.1 NUM njb-55 120 125 ° ° NOUN njb-55 121 1 c c NOUN njb-55 121 2 , , PUNCT njb-55 121 3     SPACE njb-55 121 4 64.2 64.2 NUM njb-55 121 5 ° ° NOUN njb-55 121 6 c c NOUN njb-55 121 7 , , PUNCT njb-55 121 8     SPACE njb-55 121 9 65.0 65.0 NUM njb-55 121 10 ° ° NOUN njb-55 121 11 c c NOUN njb-55 121 12     SPACE njb-55 121 13 and and CCONJ njb-55 121 14     SPACE njb-55 121 15 65.5 65.5 NUM njb-55 121 16 ° ° NOUN njb-55 122 1 c c NOUN njb-55 122 2     SPACE njb-55 122 3 ( ( PUNCT njb-55 122 4 fig fig NOUN njb-55 122 5 . . PUNCT njb-55 122 6     SPACE njb-55 122 7 1b 1b NUM njb-55 122 8 ) ) PUNCT njb-55 122 9 . . PUNCT njb-55 122 10     SPACE njb-55 123 1 the the DET njb-55 123 2     SPACE njb-55 123 3 427bp 427bp NOUN njb-55 123 4     SPACE njb-55 123 5 amplicons amplicon NOUN njb-55 123 6     SPACE njb-55 123 7 thus thus ADV njb-55 123 8     SPACE njb-55 123 9 amplified amplify VERB njb-55 123 10     SPACE njb-55 123 11 was be AUX njb-55 123 12     SPACE njb-55 123 13 subjected subject VERB njb-55 123 14     SPACE njb-55 123 15 to to PART njb-55 123 16     SPACE njb-55 123 17 re re VERB njb-55 123 18 . . PROPN njb-55 123 19     SPACE njb-55 124 1 the the DET njb-55 124 2     SPACE njb-55 124 3 restriction restriction NOUN njb-55 124 4     SPACE njb-55 124 5 enzyme enzyme NOUN njb-55 124 6     SPACE njb-55 124 7 pattern pattern NOUN njb-55 124 8     SPACE njb-55 124 9 of of ADP njb-55 124 10     SPACE njb-55 124 11 this this DET njb-55 124 12     SPACE njb-55 124 13 amplicon amplicon NOUN njb-55 124 14     SPACE njb-55 124 15 confirms confirm VERB njb-55 124 16     SPACE njb-55 124 17 it it PRON njb-55 124 18     SPACE njb-55 124 19 to to PART njb-55 124 20     SPACE njb-55 124 21 be be AUX njb-55 124 22     SPACE njb-55 124 23 a a DET njb-55 124 24     SPACE njb-55 124 25 lipl41 lipl41 PROPN njb-55 124 26     SPACE njb-55 124 27 partial partial ADJ njb-55 124 28     SPACE njb-55 124 29 gene gene NOUN njb-55 124 30     SPACE njb-55 124 31 of of ADP njb-55 124 32     SPACE njb-55 124 33 leptospira leptospira PROPN njb-55 124 34     SPACE njb-55 124 35 genus genus NOUN njb-55 124 36     SPACE njb-55 124 37 ( ( PUNCT njb-55 124 38 fig fig NOUN njb-55 124 39 . . PUNCT njb-55 124 40     SPACE njb-55 124 41 2b 2b NUM njb-55 124 42 ) ) PUNCT njb-55 124 43 . . PUNCT njb-55 124 44     SPACE njb-55 125 1 the the DET njb-55 125 2     SPACE njb-55 125 3 specificity specificity NOUN njb-55 125 4     SPACE njb-55 125 5 of of ADP njb-55 125 6     SPACE njb-55 125 7 this this DET njb-55 125 8     SPACE njb-55 125 9 primer primer NOUN njb-55 125 10     SPACE njb-55 125 11 was be AUX njb-55 125 12     SPACE njb-55 125 13 tested test VERB njb-55 125 14     SPACE njb-55 125 15 on on ADP njb-55 125 16     SPACE njb-55 125 17 nine nine NUM njb-55 125 18     SPACE njb-55 125 19 different different ADJ njb-55 125 20     SPACE njb-55 125 21 serovars serovar NOUN njb-55 125 22     SPACE njb-55 125 23 under under ADP njb-55 125 24     SPACE njb-55 125 25 optimized optimize VERB njb-55 125 26     SPACE njb-55 125 27 conditions condition NOUN njb-55 125 28 . . PUNCT njb-55 125 29     SPACE njb-55 126 1 the the DET njb-55 126 2     SPACE njb-55 126 3 pcr pcr PROPN njb-55 126 4     SPACE njb-55 126 5 assay assay NOUN njb-55 126 6     SPACE njb-55 126 7 showed show VERB njb-55 126 8     SPACE njb-55 126 9 nonspecific nonspecific ADJ njb-55 126 10     SPACE njb-55 126 11 amplification amplification NOUN njb-55 126 12     SPACE njb-55 126 13 along along ADP njb-55 126 14     SPACE njb-55 126 15 with with ADP njb-55 126 16     SPACE njb-55 126 17 the the DET njb-55 126 18     SPACE njb-55 126 19 specific specific ADJ njb-55 126 20     SPACE njb-55 126 21 band band NOUN njb-55 126 22 . . PUNCT njb-55 126 23     SPACE njb-55 127 1 primer primer NOUN njb-55 127 2     SPACE njb-55 127 3 degradation degradation NOUN njb-55 127 4     SPACE njb-55 127 5 was be AUX njb-55 127 6     SPACE njb-55 127 7 suspected suspect VERB njb-55 127 8     SPACE njb-55 127 9 and and CCONJ njb-55 127 10     SPACE njb-55 127 11 the the DET njb-55 127 12     SPACE njb-55 127 13 pcr pcr NOUN njb-55 127 14     SPACE njb-55 127 15 reactions reaction NOUN njb-55 127 16     SPACE njb-55 127 17 at at ADP njb-55 127 18     SPACE njb-55 127 19 the the DET njb-55 127 20     SPACE njb-55 127 21 optimized optimize VERB njb-55 127 22     SPACE njb-55 127 23 conditions condition NOUN njb-55 127 24     SPACE njb-55 127 25 were be AUX njb-55 127 26     SPACE njb-55 127 27 repeated repeat VERB njb-55 127 28     SPACE njb-55 127 29 with with ADP njb-55 127 30     SPACE njb-55 127 31 fresh fresh ADJ njb-55 127 32     SPACE njb-55 127 33 primer primer NOUN njb-55 127 34     SPACE njb-55 127 35 set set VERB njb-55 127 36     SPACE njb-55 127 37 p30/31 p30/31 NUM njb-55 127 38 . . PUNCT njb-55 127 39     SPACE njb-55 128 1 pcr pcr NOUN njb-55 128 2     SPACE njb-55 128 3 reactions reaction NOUN njb-55 128 4     SPACE njb-55 128 5 were be AUX njb-55 128 6     SPACE njb-55 128 7 again again ADV njb-55 128 8     SPACE njb-55 128 9 carried carry VERB njb-55 128 10     SPACE njb-55 128 11 out out ADP njb-55 128 12     SPACE njb-55 128 13 on on ADP njb-55 128 14     SPACE njb-55 128 15 nine nine NUM njb-55 128 16     SPACE njb-55 128 17 serovars serovar NOUN njb-55 128 18     SPACE njb-55 128 19 at at ADP njb-55 128 20     SPACE njb-55 128 21 mgcl2 mgcl2 PROPN njb-55 128 22     SPACE njb-55 128 23 results result NOUN njb-55 128 24     SPACE njb-55 128 25 and and CCONJ njb-55 128 26     SPACE njb-55 128 27 discussion discussion NOUN njb-55 128 28     SPACE njb-55 128 29 specificity specificity NOUN njb-55 128 30     SPACE njb-55 128 31 of of ADP njb-55 128 32     SPACE njb-55 128 33 leptospira leptospira PROPN njb-55 128 34     SPACE njb-55 128 35 spp spp PROPN njb-55 128 36 . . PUNCT njb-55 128 37     SPACE njb-55 128 38 primers primer NOUN njb-55 128 39 . . PUNCT njb-55 128 40     SPACE njb-55 129 1 initially initially ADV njb-55 129 2 , , PUNCT njb-55 129 3     SPACE njb-55 129 4 pcr pcr VERB njb-55 129 5     SPACE njb-55 129 6 using use VERB njb-55 129 7     SPACE njb-55 129 8 p28/29 p28/29 PROPN njb-55 129 9     SPACE njb-55 129 10 was be AUX njb-55 129 11     SPACE njb-55 129 12 standardized standardize VERB njb-55 129 13     SPACE njb-55 129 14 by by ADP njb-55 129 15     SPACE njb-55 129 16 carrying carry VERB njb-55 129 17     SPACE njb-55 129 18 out out ADP njb-55 129 19     SPACE njb-55 129 20 reactions reaction NOUN njb-55 129 21     SPACE njb-55 129 22 at at ADP njb-55 129 23     SPACE njb-55 129 24 various various ADJ njb-55 129 25     SPACE njb-55 129 26 annealing annealing NOUN njb-55 129 27     SPACE njb-55 129 28 temperatures temperature NOUN njb-55 129 29     SPACE njb-55 129 30 ranging range VERB njb-55 129 31     SPACE njb-55 129 32 from from ADP njb-55 129 33     SPACE njb-55 129 34 58 58 NUM njb-55 129 35 ° ° PUNCT njb-55 129 36 c c NOUN njb-55 129 37     SPACE njb-55 129 38 to to ADP njb-55 129 39     SPACE njb-55 129 40 68 68 NUM njb-55 129 41 ° ° NOUN njb-55 129 42 c c NOUN njb-55 129 43     SPACE njb-55 129 44 on on ADP njb-55 129 45     SPACE njb-55 129 46 l. l. NOUN njb-55 129 47     SPACE njb-55 129 48 icterohaemorrhagiae icterohaemorrhagiae NOUN njb-55 129 49     SPACE njb-55 129 50 serovar serovar NOUN njb-55 129 51     SPACE njb-55 129 52 which which PRON njb-55 129 53     SPACE njb-55 129 54 showed show VERB njb-55 129 55     SPACE njb-55 129 56 amplification amplification NOUN njb-55 129 57     SPACE njb-55 129 58 at at ADP njb-55 129 59     SPACE njb-55 129 60 58 58 NUM njb-55 129 61 ° ° NOUN njb-55 129 62 c c NOUN njb-55 129 63 , , PUNCT njb-55 129 64     SPACE njb-55 129 65 58.7 58.7 NUM njb-55 129 66 ° ° NOUN njb-55 129 67 c c NOUN njb-55 129 68     SPACE njb-55 129 69 and and CCONJ njb-55 129 70     SPACE njb-55 129 71 60.8 60.8 NUM njb-55 129 72 ° ° NOUN njb-55 129 73 c c NOUN njb-55 129 74     SPACE njb-55 129 75 ( ( PUNCT njb-55 129 76 fig.1a fig.1a NOUN njb-55 129 77 ) ) PUNCT njb-55 129 78 . . PUNCT njb-55 129 79     SPACE njb-55 130 1 a a DET njb-55 130 2     SPACE njb-55 130 3 single single ADJ njb-55 130 4     SPACE njb-55 130 5 band band NOUN njb-55 130 6     SPACE njb-55 130 7 of of ADP njb-55 130 8     SPACE njb-55 130 9 approximately approximately ADV njb-55 130 10     SPACE njb-55 130 11 385bp 385bp PROPN njb-55 130 12     SPACE njb-55 130 13 was be AUX njb-55 130 14     SPACE njb-55 130 15 observed observe VERB njb-55 130 16     SPACE njb-55 130 17 at at ADP njb-55 130 18     SPACE njb-55 130 19 60 60 NUM njb-55 130 20 ° ° NOUN njb-55 130 21 c c NOUN njb-55 130 22     SPACE njb-55 130 23 annealing anneal VERB njb-55 130 24     SPACE njb-55 130 25 temperature temperature NOUN njb-55 130 26 . . PUNCT njb-55 130 27     SPACE njb-55 131 1 a a DET njb-55 131 2     SPACE njb-55 131 3 set set NOUN njb-55 131 4     SPACE njb-55 131 5 of of ADP njb-55 131 6     SPACE njb-55 131 7 pcr pcr NOUN njb-55 131 8     SPACE njb-55 131 9 reactions reaction NOUN njb-55 131 10     SPACE njb-55 131 11 were be AUX njb-55 131 12     SPACE njb-55 131 13 done do VERB njb-55 131 14     SPACE njb-55 131 15 by by ADP njb-55 131 16     SPACE njb-55 131 17 varying vary VERB njb-55 131 18     SPACE njb-55 131 19 the the DET njb-55 131 20     SPACE njb-55 131 21 template template NOUN njb-55 131 22     SPACE njb-55 131 23 concentration concentration NOUN njb-55 131 24     SPACE njb-55 131 25 from from ADP njb-55 131 26     SPACE njb-55 131 27 5ng 5ng NOUN njb-55 131 28     SPACE njb-55 131 29 to to ADP njb-55 131 30     SPACE njb-55 131 31 60ngand 60ngand NOUN njb-55 131 32     SPACE njb-55 131 33 could could AUX njb-55 131 34     SPACE njb-55 131 35 amplify amplify VERB njb-55 131 36     SPACE njb-55 131 37 the the DET njb-55 131 38     SPACE njb-55 131 39 specific specific ADJ njb-55 131 40     SPACE njb-55 131 41 band band NOUN njb-55 131 42     SPACE njb-55 131 43 at at ADP njb-55 131 44     SPACE njb-55 131 45 all all DET njb-55 131 46     SPACE njb-55 131 47 the the DET njb-55 131 48     SPACE njb-55 131 49 template template NOUN njb-55 131 50     SPACE njb-55 131 51 concentrations concentration NOUN njb-55 131 52 . . PUNCT njb-55 131 53     SPACE njb-55 132 1 the the DET njb-55 132 2     SPACE njb-55 132 3 pcr pcr NOUN njb-55 132 4     SPACE njb-55 132 5 products product NOUN njb-55 132 6     SPACE njb-55 132 7 were be AUX njb-55 132 8     SPACE njb-55 132 9 pooled pool VERB njb-55 132 10     SPACE njb-55 132 11 and and CCONJ njb-55 132 12     SPACE njb-55 132 13 characterized characterize VERB njb-55 132 14     SPACE njb-55 132 15 by by ADP njb-55 132 16     SPACE njb-55 132 17 subjecting subject VERB njb-55 132 18     SPACE njb-55 132 19 to to PART njb-55 132 20     SPACE njb-55 132 21 re re VERB njb-55 132 22     SPACE njb-55 132 23 digestion digestion NOUN njb-55 132 24     SPACE njb-55 132 25 using use VERB njb-55 132 26     SPACE njb-55 132 27 rsai rsai PROPN njb-55 132 28     SPACE njb-55 132 29 and and CCONJ njb-55 132 30     SPACE njb-55 132 31 hindiii hindiii PROPN njb-55 132 32     SPACE njb-55 132 33 restriction restriction NOUN njb-55 132 34     SPACE njb-55 132 35 enzymes enzyme NOUN njb-55 132 36 , , PUNCT njb-55 132 37     SPACE njb-55 132 38 both both PRON njb-55 132 39     SPACE njb-55 132 40 of of ADP njb-55 132 41     SPACE njb-55 132 42 which which PRON njb-55 132 43     SPACE njb-55 132 44 confirmed confirm VERB njb-55 132 45     SPACE njb-55 132 46 the the DET njb-55 132 47     SPACE njb-55 132 48 product product NOUN njb-55 132 49     SPACE njb-55 132 50 size size NOUN njb-55 132 51     SPACE njb-55 132 52 of of ADP njb-55 132 53     SPACE njb-55 132 54 p28/29 p28/29 NOUN njb-55 132 55     SPACE njb-55 132 56 to to PART njb-55 132 57     SPACE njb-55 132 58 be be AUX njb-55 132 59     SPACE njb-55 132 60 385bp 385bp ADV njb-55 132 61     SPACE njb-55 132 62 ( ( PUNCT njb-55 132 63 fig.2a fig.2a VERB njb-55 132 64 ) ) PUNCT njb-55 132 65 . . PUNCT njb-55 132 66     SPACE njb-55 133 1 the the DET njb-55 133 2     SPACE njb-55 133 3 specificity specificity NOUN njb-55 133 4     SPACE njb-55 133 5 of of ADP njb-55 133 6     SPACE njb-55 133 7 this this DET njb-55 133 8     SPACE njb-55 133 9 primer primer NOUN njb-55 133 10     SPACE njb-55 133 11 was be AUX njb-55 133 12     SPACE njb-55 133 13 tested test VERB njb-55 133 14     SPACE njb-55 133 15 on on ADP njb-55 133 16     SPACE njb-55 133 17 nine nine NUM njb-55 133 18     SPACE njb-55 133 19 different different ADJ njb-55 133 20     SPACE njb-55 133 21 serovars serovar NOUN njb-55 133 22     SPACE njb-55 133 23 namely namely ADV njb-55 133 24     SPACE njb-55 133 25 l. l. NOUN njb-55 133 26     SPACE njb-55 133 27 icterohaemorrhagiae icterohaemorrhagiae NOUN njb-55 133 28 , , PUNCT njb-55 133 29     SPACE njb-55 133 30 l. l. PROPN njb-55 133 31     SPACE njb-55 133 32 canicola canicola PROPN njb-55 133 33 , , PUNCT njb-55 133 34     SPACE njb-55 133 35 l. l. PROPN njb-55 133 36     SPACE njb-55 133 37 pamona pamona PROPN njb-55 133 38 , , PUNCT njb-55 133 39     SPACE njb-55 133 40 l. l. PROPN njb-55 133 41     SPACE njb-55 133 42 autumnalis autumnalis PROPN njb-55 133 43 , , PUNCT njb-55 133 44     SPACE njb-55 133 45 l. l. PROPN njb-55 133 46     SPACE njb-55 133 47 javanica javanica PROPN njb-55 133 48 , , PUNCT njb-55 133 49     SPACE njb-55 133 50 l. l. PROPN njb-55 133 51     SPACE njb-55 133 52 pyrogenes pyrogene NOUN njb-55 133 53 , , PUNCT njb-55 133 54     SPACE njb-55 133 55 l. l. PROPN njb-55 133 56     SPACE njb-55 133 57 australis australi NOUN njb-55 133 58 , , PUNCT njb-55 133 59     SPACE njb-55 133 60 l. l. PROPN njb-55 133 61     SPACE njb-55 133 62 hardjo hardjo PROPN njb-55 133 63     SPACE njb-55 133 64 and and CCONJ njb-55 133 65     SPACE njb-55 133 66 l. l. PROPN njb-55 133 67     SPACE njb-55 133 68 inadai inadai PROPN njb-55 133 69     SPACE njb-55 133 70 under under ADP njb-55 133 71     SPACE njb-55 133 72 standardized standardize VERB njb-55 133 73     SPACE njb-55 133 74 condition condition NOUN njb-55 133 75 . . PUNCT njb-55 133 76     SPACE njb-55 134 1 the the DET njb-55 134 2     SPACE njb-55 134 3 assay assay NOUN njb-55 134 4     SPACE njb-55 134 5 targeting target VERB njb-55 134 6     SPACE njb-55 134 7 the the DET njb-55 134 8     SPACE njb-55 134 9 partial partial ADJ njb-55 134 10     SPACE njb-55 134 11 sequence sequence NOUN njb-55 134 12     SPACE njb-55 134 13 of of ADP njb-55 134 14     SPACE njb-55 134 15 lipl21 lipl21 NOUN njb-55 134 16     SPACE njb-55 134 17 was be AUX njb-55 134 18     SPACE njb-55 134 19 found find VERB njb-55 134 20     SPACE njb-55 134 21 to to PART njb-55 134 22     SPACE njb-55 134 23 be be AUX njb-55 134 24     SPACE njb-55 134 25 specific specific ADJ njb-55 134 26     SPACE njb-55 134 27 for for ADP njb-55 134 28     SPACE njb-55 134 29 eight eight NUM njb-55 134 30     SPACE njb-55 134 31 pathogenic pathogenic ADJ njb-55 134 32     SPACE njb-55 134 33 leptospires leptospire NOUN njb-55 134 34     SPACE njb-55 134 35 out out ADP njb-55 134 36     SPACE njb-55 134 37 of of ADP njb-55 134 38     SPACE njb-55 134 39 nine nine NUM njb-55 134 40       SPACE njb-55 134 41 leptospires leptospire NOUN njb-55 134 42     SPACE njb-55 134 43 tested test VERB njb-55 134 44     SPACE njb-55 134 45 ( ( PUNCT njb-55 134 46 fig fig NOUN njb-55 134 47 . . PUNCT njb-55 134 48     SPACE njb-55 134 49 3a 3a NUM njb-55 134 50 ) ) PUNCT njb-55 134 51     SPACE njb-55 134 52 for for SCONJ njb-55 134 53     SPACE njb-55 134 54 the the DET njb-55 134 55     SPACE njb-55 134 56 pcr pcr NOUN njb-55 134 57     SPACE njb-55 134 58 assay assay NOUN njb-55 134 59     SPACE njb-55 134 60 targeted target VERB njb-55 134 61     SPACE njb-55 134 62 to to ADP njb-55 134 63     SPACE njb-55 134 64 the the DET njb-55 134 65     SPACE njb-55 134 66 partial partial ADJ njb-55 134 67     SPACE njb-55 134 68 sequence sequence NOUN njb-55 134 69     SPACE njb-55 134 70 of of ADP njb-55 134 71     SPACE njb-55 134 72 lipl41 lipl41 PROPN njb-55 134 73 , , PUNCT njb-55 134 74     SPACE njb-55 134 75 standardization standardization NOUN njb-55 134 76     SPACE njb-55 134 77 was be AUX njb-55 134 78     SPACE njb-55 134 79 done do VERB njb-55 134 80     SPACE njb-55 134 81 initially initially ADV njb-55 134 82     SPACE njb-55 134 83 by by ADP njb-55 134 84     SPACE njb-55 134 85 carrying carry VERB njb-55 134 86     SPACE njb-55 134 87 figure figure NOUN njb-55 134 88     SPACE njb-55 134 89 1 1 NUM njb-55 134 90 . . PUNCT njb-55 134 91     SPACE njb-55 134 92 ( ( PUNCT njb-55 134 93 a a X njb-55 134 94 ) ) PUNCT njb-55 134 95     SPACE njb-55 134 96 specificity specificity NOUN njb-55 134 97     SPACE njb-55 134 98 and and CCONJ njb-55 134 99     SPACE njb-55 134 100 detection detection NOUN njb-55 134 101     SPACE njb-55 134 102 of of ADP njb-55 134 103     SPACE njb-55 134 104 annealing anneal VERB njb-55 134 105     SPACE njb-55 134 106 temperature temperature NOUN njb-55 134 107     SPACE njb-55 134 108 of of ADP njb-55 134 109     SPACE njb-55 134 110 lipl21 lipl21 NOUN njb-55 134 111     SPACE njb-55 134 112 gene gene NOUN njb-55 134 113 . . PUNCT njb-55 134 114     SPACE njb-55 135 1 annealing anneal VERB njb-55 135 2     SPACE njb-55 135 3 temperature temperature NOUN njb-55 135 4     SPACE njb-55 135 5 was be AUX njb-55 135 6     SPACE njb-55 135 7 standardized standardize VERB njb-55 135 8     SPACE njb-55 135 9 to to ADP njb-55 135 10     SPACE njb-55 135 11 600c 600c NOUN njb-55 135 12     SPACE njb-55 135 13 for for ADP njb-55 135 14     SPACE njb-55 135 15 1 1 NUM njb-55 135 16     SPACE njb-55 135 17 min min PROPN njb-55 135 18 . . PROPN njb-55 135 19         SPACE njb-55 135 20 lane1‐6 lane1‐6 PROPN njb-55 135 21 , , PUNCT njb-55 135 22     SPACE njb-55 135 23 pcr pcr NOUN njb-55 135 24     SPACE njb-55 135 25 products product NOUN njb-55 135 26     SPACE njb-55 135 27 using use VERB njb-55 135 28     SPACE njb-55 135 29 dna dna NOUN njb-55 135 30     SPACE njb-55 135 31 template template NOUN njb-55 135 32     SPACE njb-55 135 33 of of ADP njb-55 135 34     SPACE njb-55 135 35 l. l. NOUN njb-55 135 36     SPACE njb-55 135 37 icterohaemorrhagiae icterohaemorrhagiae NOUN njb-55 135 38 . . PUNCT njb-55 135 39         SPACE njb-55 136 1 lane lane NOUN njb-55 136 2     SPACE njb-55 136 3 1‐6 1‐6 NUM njb-55 136 4     SPACE njb-55 136 5 annealing annealing NOUN njb-55 136 6     SPACE njb-55 136 7 temperature temperature NOUN njb-55 136 8     SPACE njb-55 136 9 of of ADP njb-55 136 10     SPACE njb-55 136 11 each each DET njb-55 136 12     SPACE njb-55 136 13 lane lane NOUN njb-55 136 14     SPACE njb-55 136 15 corresponding correspond VERB njb-55 136 16     SPACE njb-55 136 17 to to ADP njb-55 136 18     SPACE njb-55 136 19 58oc 58oc NOUN njb-55 136 20 , , PUNCT njb-55 136 21     SPACE njb-55 136 22 58.7oc 58.7oc NUM njb-55 136 23 , , PUNCT njb-55 136 24     SPACE njb-55 136 25 60.8oc 60.8oc NUM njb-55 136 26 , , PUNCT njb-55 136 27     SPACE njb-55 136 28 63.5oc 63.5oc PROPN njb-55 136 29 , , PUNCT njb-55 136 30     SPACE njb-55 136 31 66.1oc 66.1oc PROPN njb-55 136 32 , , PUNCT njb-55 136 33     SPACE njb-55 136 34 68oc 68oc PROPN njb-55 136 35 , , PUNCT njb-55 136 36     SPACE njb-55 136 37 respectively respectively ADV njb-55 136 38 . . PUNCT njb-55 136 39       SPACE njb-55 137 1 lane lane NOUN njb-55 137 2     SPACE njb-55 137 3 7 7 NUM njb-55 137 4 , , PUNCT njb-55 137 5     SPACE njb-55 137 6 100bp 100bp PROPN njb-55 137 7     SPACE njb-55 137 8 dna dna PROPN njb-55 137 9     SPACE njb-55 137 10 ladder ladder NOUN njb-55 137 11     SPACE njb-55 137 12 ( ( PUNCT njb-55 137 13 fermentas fermenta NOUN njb-55 137 14 ) ) PUNCT njb-55 137 15 . . PUNCT njb-55 137 16         SPACE njb-55 138 1 gel gel NOUN njb-55 138 2     SPACE njb-55 138 3 run run NOUN njb-55 138 4     SPACE njb-55 138 5 performed perform VERB njb-55 138 6     SPACE njb-55 138 7 using use VERB njb-55 138 8     SPACE njb-55 138 9 2 2 NUM njb-55 138 10 % % NOUN njb-55 138 11     SPACE njb-55 138 12 agarose agarose NOUN njb-55 138 13 . . PUNCT njb-55 138 14     SPACE njb-55 139 1 figure figure VERB njb-55 139 2     SPACE njb-55 139 3 1 1 NUM njb-55 139 4 . . PUNCT njb-55 139 5     SPACE njb-55 139 6 ( ( PUNCT njb-55 139 7 b b X njb-55 139 8 ) ) PUNCT njb-55 139 9     SPACE njb-55 139 10 specificity specificity NOUN njb-55 139 11     SPACE njb-55 139 12 and and CCONJ njb-55 139 13     SPACE njb-55 139 14 detection detection NOUN njb-55 139 15     SPACE njb-55 139 16 of of ADP njb-55 139 17     SPACE njb-55 139 18 annealing anneal VERB njb-55 139 19     SPACE njb-55 139 20 temperature temperature NOUN njb-55 139 21     SPACE njb-55 139 22 of of ADP njb-55 139 23     SPACE njb-55 139 24 lipl41 lipl41 PROPN njb-55 139 25     SPACE njb-55 139 26 gene gene NOUN njb-55 139 27 . . PUNCT njb-55 139 28     SPACE njb-55 140 1 annealing anneal VERB njb-55 140 2     SPACE njb-55 140 3 temperature temperature NOUN njb-55 140 4     SPACE njb-55 140 5 was be AUX njb-55 140 6     SPACE njb-55 140 7 standardized standardize VERB njb-55 140 8     SPACE njb-55 140 9 to to ADP njb-55 140 10     SPACE njb-55 140 11 600c 600c DET njb-55 140 12     SPACE njb-55 140 13 for for ADP njb-55 140 14     SPACE njb-55 140 15 1 1 NUM njb-55 140 16     SPACE njb-55 140 17 min min PROPN njb-55 140 18 . . PROPN njb-55 140 19         SPACE njb-55 140 20 lane2‐7 lane2‐7 PROPN njb-55 140 21 , , PUNCT njb-55 140 22     SPACE njb-55 140 23 pcr pcr NOUN njb-55 140 24     SPACE njb-55 140 25 products product NOUN njb-55 140 26     SPACE njb-55 140 27 using use VERB njb-55 140 28     SPACE njb-55 140 29 dna dna NOUN njb-55 140 30     SPACE njb-55 140 31 template template NOUN njb-55 140 32     SPACE njb-55 140 33 of of ADP njb-55 140 34     SPACE njb-55 140 35 l. l. PROPN njb-55 140 36     SPACE njb-55 140 37 javanica javanica PROPN njb-55 140 38 . . PUNCT njb-55 140 39       SPACE njb-55 140 40 lane lane NOUN njb-55 140 41     SPACE njb-55 140 42 2‐ 2‐ NUM njb-55 140 43 7 7 NUM njb-55 140 44     SPACE njb-55 140 45 annealing anneal VERB njb-55 140 46     SPACE njb-55 140 47 temperature temperature NOUN njb-55 140 48     SPACE njb-55 140 49 of of ADP njb-55 140 50     SPACE njb-55 140 51 each each DET njb-55 140 52     SPACE njb-55 140 53 lane lane NOUN njb-55 140 54     SPACE njb-55 140 55 corresponding correspond VERB njb-55 140 56     SPACE njb-55 140 57 to to ADP njb-55 140 58     SPACE njb-55 140 59 60.5oc 60.5oc NUM njb-55 140 60 , , PUNCT njb-55 140 61     SPACE njb-55 140 62 61.8oc 61.8oc PROPN njb-55 140 63 , , PUNCT njb-55 140 64     SPACE njb-55 140 65 63.1oc 63.1oc PROPN njb-55 140 66 , , PUNCT njb-55 140 67     SPACE njb-55 140 68 64.2oc 64.2oc NUM njb-55 140 69 , , PUNCT njb-55 140 70     SPACE njb-55 140 71 65.0oc 65.0oc PROPN njb-55 140 72 , , PUNCT njb-55 140 73     SPACE njb-55 140 74 65.6oc 65.6oc PROPN njb-55 140 75 , , PUNCT njb-55 140 76     SPACE njb-55 140 77 respectively respectively ADV njb-55 140 78 . . PUNCT njb-55 140 79         SPACE njb-55 141 1 lane lane NOUN njb-55 141 2     SPACE njb-55 141 3 1 1 NUM njb-55 141 4     SPACE njb-55 141 5 and and CCONJ njb-55 141 6     SPACE njb-55 141 7 8 8 NUM njb-55 141 8 , , PUNCT njb-55 141 9     SPACE njb-55 141 10 100bp 100bp PROPN njb-55 141 11     SPACE njb-55 141 12 dna dna PROPN njb-55 141 13     SPACE njb-55 141 14 ladder ladder NOUN njb-55 141 15     SPACE njb-55 141 16 ( ( PUNCT njb-55 141 17 fermentas fermenta NOUN njb-55 141 18 ) ) PUNCT njb-55 141 19 . . PUNCT njb-55 141 20       SPACE njb-55 142 1 gel gel NOUN njb-55 142 2     SPACE njb-55 142 3 run run NOUN njb-55 142 4     SPACE njb-55 142 5 performed perform VERB njb-55 142 6     SPACE njb-55 142 7 using use VERB njb-55 142 8     SPACE njb-55 142 9 2 2 NUM njb-55 142 10 % % NOUN njb-55 142 11     SPACE njb-55 142 12 agarose agarose NOUN njb-55 142 13 . . PUNCT njb-55 142 14     SPACE njb-55 142 15 nepal nepal ADJ njb-55 142 16     SPACE njb-55 142 17 journal journal NOUN njb-55 142 18     SPACE njb-55 142 19 of of ADP njb-55 142 20     SPACE njb-55 142 21 biotechnology biotechnology NOUN njb-55 142 22 . . PUNCT njb-55 142 23     SPACE njb-55 143 1 jan jan PROPN njb-55 143 2 . . PROPN njb-55 143 3     SPACE njb-55 143 4 2011 2011 NUM njb-55 143 5 , , PUNCT njb-55 143 6     SPACE njb-55 143 7 vol vol NOUN njb-55 143 8 . . PUNCT njb-55 143 9     SPACE njb-55 143 10 1 1 NUM njb-55 143 11 , , PUNCT njb-55 143 12     SPACE njb-55 143 13 no no INTJ njb-55 143 14 . . PUNCT njb-55 143 15     SPACE njb-55 143 16 1 1 NUM njb-55 143 17 : : PUNCT njb-55 143 18     SPACE njb-55 143 19 22‐30 22‐30 NUM njb-55 143 20     SPACE njb-55 143 21 27 27 NUM njb-55 143 22     SPACE njb-55 143 23 biotechnology biotechnology NOUN njb-55 143 24     SPACE njb-55 143 25 society society NOUN njb-55 143 26     SPACE njb-55 143 27 of of ADP njb-55 143 28     SPACE njb-55 143 29 nepal nepal NOUN njb-55 143 30     SPACE njb-55 143 31 ( ( PUNCT njb-55 143 32 bsn bsn PROPN njb-55 143 33 ) ) PUNCT njb-55 143 34 , , PUNCT njb-55 143 35     SPACE njb-55 143 36 all all DET njb-55 143 37     SPACE njb-55 143 38 rights right NOUN njb-55 143 39     SPACE njb-55 143 40 reserved reserve VERB njb-55 143 41             SPACE njb-55 143 42 organism organism NOUN njb-55 143 43     SPACE njb-55 143 44 pcr pcr PROPN njb-55 143 45     SPACE njb-55 143 46 reaction reaction NOUN njb-55 143 47     SPACE njb-55 143 48 mixture mixture NOUN njb-55 143 49     SPACE njb-55 143 50 ( ( PUNCT njb-55 143 51 25ml 25ml NOUN njb-55 143 52 ) ) PUNCT njb-55 143 53                 SPACE njb-55 143 54 p28 p28 NOUN njb-55 143 55     SPACE njb-55 143 56 /29 /29 PUNCT njb-55 143 57           SPACE njb-55 143 58 p30/31 p30/31 PROPN njb-55 143 59           SPACE njb-55 143 60 l. l. PROPN njb-55 143 61     SPACE njb-55 143 62 javanica javanica PROPN njb-55 143 63     SPACE njb-55 143 64 l. l. PROPN njb-55 143 65     SPACE njb-55 143 66 autumnalis autumnalis PROPN njb-55 143 67     SPACE njb-55 143 68 l. l. PROPN njb-55 143 69     SPACE njb-55 143 70 hardjo hardjo PROPN njb-55 143 71     SPACE njb-55 143 72 l. l. PROPN njb-55 143 73     SPACE njb-55 143 74 pyrogenes pyrogene VERB njb-55 143 75     SPACE njb-55 143 76 l. l. PROPN njb-55 143 77     SPACE njb-55 143 78 pamona pamona PROPN njb-55 143 79     SPACE njb-55 143 80 l. l. PROPN njb-55 143 81     SPACE njb-55 143 82 australis australis PROPN njb-55 143 83     SPACE njb-55 143 84 l. l. PROPN njb-55 143 85     SPACE njb-55 143 86 icterohaemorrhagiae icterohaemorrhagiae PROPN njb-55 143 87     SPACE njb-55 143 88 l. l. PROPN njb-55 143 89     SPACE njb-55 143 90 inadai inadai PROPN njb-55 143 91     SPACE njb-55 143 92 l. l. PROPN njb-55 143 93     SPACE njb-55 143 94 canicola canicola PROPN njb-55 143 95     SPACE njb-55 143 96 10x 10x NOUN njb-55 143 97     SPACE njb-55 143 98 buffer buffer NOUN njb-55 143 99                                               SPACE njb-55 143 100 2.50ml 2.50ml NUM njb-55 143 101     SPACE njb-55 143 102 mgcl2 mgcl2 NOUN njb-55 143 103                                                                                               SPACE njb-55 143 104 1.50ml 1.50ml NUM njb-55 143 105     SPACE njb-55 143 106 dntp dntp ADJ njb-55 143 107     SPACE njb-55 143 108 mix mix NOUN njb-55 143 109                                                                             SPACE njb-55 143 110 2.00ml 2.00ml NUM njb-55 143 111     SPACE njb-55 143 112 forward forward ADV njb-55 143 113     SPACE njb-55 143 114 primer primer NOUN njb-55 143 115                                           SPACE njb-55 143 116 0.50ml 0.50ml PUNCT njb-55 143 117     SPACE njb-55 143 118 reverse reverse NOUN njb-55 143 119     SPACE njb-55 143 120 primer primer NOUN njb-55 143 121                                             SPACE njb-55 143 122 0.50ml 0.50ml PUNCT njb-55 143 123     SPACE njb-55 143 124 template template NOUN njb-55 143 125                                                                               SPACE njb-55 143 126 0.25ml 0.25ml PROPN njb-55 143 127     SPACE njb-55 143 128 taq taq PROPN njb-55 143 129     SPACE njb-55 143 130 pol pol PROPN njb-55 143 131                                                                   SPACE njb-55 143 132 2.00ml 2.00ml NUM njb-55 143 133     SPACE njb-55 143 134 nuclease nuclease ADJ njb-55 143 135     SPACE njb-55 143 136 free free ADJ njb-55 143 137     SPACE njb-55 143 138 water water NOUN njb-55 143 139             SPACE njb-55 143 140 15.75ml 15.75ml NUM njb-55 143 141           SPACE njb-55 143 142 lipl21 lipl21 PROPN njb-55 143 143     SPACE njb-55 143 144 lipl41 lipl41 PROPN njb-55 143 145     SPACE njb-55 143 146 buffer buffer NOUN njb-55 143 147     SPACE njb-55 143 148 ( ( PUNCT njb-55 143 149 10x 10x NUM njb-55 143 150 ) ) PUNCT njb-55 143 151     SPACE njb-55 143 152 enzyme enzyme NOUN njb-55 143 153     SPACE njb-55 143 154 dna dna PROPN njb-55 143 155     SPACE njb-55 143 156 water water NOUN njb-55 143 157                 SPACE njb-55 143 158 2.0 2.0 NUM njb-55 143 159     SPACE njb-55 143 160 ml ml PROPN njb-55 143 161                 SPACE njb-55 143 162 1.0 1.0 NUM njb-55 143 163     SPACE njb-55 143 164 ml ml PROPN njb-55 143 165                 SPACE njb-55 143 166 8.0 8.0 NUM njb-55 143 167     SPACE njb-55 143 168 ml ml PROPN njb-55 143 169             SPACE njb-55 143 170 11.0 11.0 NUM njb-55 143 171     SPACE njb-55 143 172 ml ml PROPN njb-55 143 173                 SPACE njb-55 143 174 2.0 2.0 NUM njb-55 143 175     SPACE njb-55 143 176 ml ml PROPN njb-55 143 177                 SPACE njb-55 143 178 2.0 2.0 NUM njb-55 143 179     SPACE njb-55 143 180 ml ml PROPN njb-55 143 181                 SPACE njb-55 143 182 5.0 5.0 NUM njb-55 143 183     SPACE njb-55 143 184 ml ml PROPN njb-55 143 185                 SPACE njb-55 143 186 10.0 10.0 NUM njb-55 143 187     SPACE njb-55 143 188 ml ml PROPN njb-55 143 189                     SPACE njb-55 143 190 total total ADJ njb-55 143 191             SPACE njb-55 143 192 20.0 20.0 NUM njb-55 143 193     SPACE njb-55 143 194 ml ml PROPN njb-55 143 195               SPACE njb-55 143 196 20.0 20.0 NUM njb-55 143 197     SPACE njb-55 143 198 ml ml PROPN njb-55 143 199     SPACE njb-55 143 200 table table NOUN njb-55 143 201     SPACE njb-55 143 202 4 4 NUM njb-55 143 203 . . PUNCT njb-55 143 204     SPACE njb-55 144 1 details detail NOUN njb-55 144 2     SPACE njb-55 144 3 of of ADP njb-55 144 4     SPACE njb-55 144 5 the the DET njb-55 144 6     SPACE njb-55 144 7 standardized standardized ADJ njb-55 144 8     SPACE njb-55 144 9 conditions condition NOUN njb-55 144 10     SPACE njb-55 144 11 for for ADP njb-55 144 12     SPACE njb-55 144 13 pcr pcr NOUN njb-55 144 14     SPACE njb-55 144 15 table table NOUN njb-55 144 16     SPACE njb-55 144 17 5 5 NUM njb-55 144 18 . . PUNCT njb-55 144 19     SPACE njb-55 145 1 details detail NOUN njb-55 145 2     SPACE njb-55 145 3 of of ADP njb-55 145 4     SPACE njb-55 145 5 the the DET njb-55 145 6     SPACE njb-55 145 7 restriction restriction NOUN njb-55 145 8     SPACE njb-55 145 9 enzyme enzyme NOUN njb-55 145 10     SPACE njb-55 145 11 digestion digestion NOUN njb-55 145 12     SPACE njb-55 145 13 reaction reaction NOUN njb-55 145 14     SPACE njb-55 145 15 for for ADP njb-55 145 16     SPACE njb-55 145 17 insert insert NOUN njb-55 145 18     SPACE njb-55 145 19 release release NOUN njb-55 145 20     SPACE njb-55 145 21 figure figure NOUN njb-55 145 22     SPACE njb-55 145 23 2 2 NUM njb-55 145 24 . . PUNCT njb-55 145 25     SPACE njb-55 145 26 ( ( PUNCT njb-55 145 27 a a X njb-55 145 28 ) ) PUNCT njb-55 145 29     SPACE njb-55 145 30 characterization characterization NOUN njb-55 145 31     SPACE njb-55 145 32 of of ADP njb-55 145 33     SPACE njb-55 145 34 amplicons amplicon NOUN njb-55 145 35     SPACE njb-55 145 36 using use VERB njb-55 145 37     SPACE njb-55 145 38 restriction restriction NOUN njb-55 145 39     SPACE njb-55 145 40 enzymes enzyme NOUN njb-55 145 41     SPACE njb-55 145 42 rsai rsai VERB njb-55 145 43     SPACE njb-55 145 44 in in ADP njb-55 145 45     SPACE njb-55 145 46 lane lane NOUN njb-55 145 47     SPACE njb-55 145 48 2 2 NUM njb-55 145 49     SPACE njb-55 145 50 and and CCONJ njb-55 145 51     SPACE njb-55 145 52 hindiii hindiii PROPN njb-55 145 53     SPACE njb-55 145 54 in in ADP njb-55 145 55     SPACE njb-55 145 56 lane lane NOUN njb-55 145 57     SPACE njb-55 145 58 3 3 NUM njb-55 145 59     SPACE njb-55 145 60 and and CCONJ njb-55 145 61     SPACE njb-55 145 62 lane lane NOUN njb-55 145 63     SPACE njb-55 145 64 dna dna NOUN njb-55 145 65     SPACE njb-55 145 66 template template NOUN njb-55 145 67     SPACE njb-55 145 68 without without ADP njb-55 145 69     SPACE njb-55 145 70 re re ADJ njb-55 145 71     SPACE njb-55 145 72 enzyme enzyme NOUN njb-55 145 73     SPACE njb-55 145 74 in in ADP njb-55 145 75     SPACE njb-55 145 76 lane lane NOUN njb-55 145 77     SPACE njb-55 145 78 1 1 NUM njb-55 145 79 , , PUNCT njb-55 145 80     SPACE njb-55 145 81 lane lane NOUN njb-55 145 82     SPACE njb-55 145 83 4 4 NUM njb-55 145 84 , , PUNCT njb-55 145 85     SPACE njb-55 145 86 100bp 100bp PROPN njb-55 145 87     SPACE njb-55 145 88 gene gene NOUN njb-55 145 89     SPACE njb-55 145 90 ruler ruler NOUN njb-55 145 91 . . PUNCT njb-55 145 92             SPACE njb-55 146 1 gel gel NOUN njb-55 146 2     SPACE njb-55 146 3 run run NOUN njb-55 146 4     SPACE njb-55 146 5 performed perform VERB njb-55 146 6     SPACE njb-55 146 7 using use VERB njb-55 146 8     SPACE njb-55 146 9 2 2 NUM njb-55 146 10 % % NOUN njb-55 146 11     SPACE njb-55 146 12 agarose agarose NOUN njb-55 146 13 . . PUNCT njb-55 146 14     SPACE njb-55 147 1 figure figure VERB njb-55 147 2     SPACE njb-55 147 3 2 2 NUM njb-55 147 4 . . PUNCT njb-55 147 5     SPACE njb-55 147 6 ( ( PUNCT njb-55 147 7 b b X njb-55 147 8 ) ) PUNCT njb-55 147 9     SPACE njb-55 147 10 characterization characterization NOUN njb-55 147 11     SPACE njb-55 147 12 of of ADP njb-55 147 13     SPACE njb-55 147 14 amplicons amplicon NOUN njb-55 147 15     SPACE njb-55 147 16 using use VERB njb-55 147 17     SPACE njb-55 147 18 restriction restriction NOUN njb-55 147 19     SPACE njb-55 147 20 enzymes enzyme NOUN njb-55 147 21     SPACE njb-55 147 22 clai clai PROPN njb-55 147 23       SPACE njb-55 147 24 in in ADP njb-55 147 25     SPACE njb-55 147 26 lane lane NOUN njb-55 147 27     SPACE njb-55 147 28 3 3 NUM njb-55 147 29 , , PUNCT njb-55 147 30     SPACE njb-55 147 31 taqi taqi VERB njb-55 147 32     SPACE njb-55 147 33 in in ADP njb-55 147 34     SPACE njb-55 147 35 lane lane NOUN njb-55 147 36     SPACE njb-55 147 37 3 3 NUM njb-55 147 38     SPACE njb-55 147 39 and and CCONJ njb-55 147 40     SPACE njb-55 147 41 rsai rsai PROPN njb-55 147 42     SPACE njb-55 147 43 in in ADP njb-55 147 44     SPACE njb-55 147 45 lane lane NOUN njb-55 147 46     SPACE njb-55 147 47 5 5 NUM njb-55 147 48 , , PUNCT njb-55 147 49     SPACE njb-55 147 50 dna dna NOUN njb-55 147 51     SPACE njb-55 147 52 template template NOUN njb-55 147 53     SPACE njb-55 147 54 with with ADP njb-55 147 55     SPACE njb-55 147 56 out out ADP njb-55 147 57     SPACE njb-55 147 58 re re ADJ njb-55 147 59     SPACE njb-55 147 60 enzyme enzyme NOUN njb-55 147 61     SPACE njb-55 147 62 in in ADP njb-55 147 63     SPACE njb-55 147 64 lane lane NOUN njb-55 147 65     SPACE njb-55 147 66 2 2 NUM njb-55 147 67 , , PUNCT njb-55 147 68     SPACE njb-55 147 69 lane lane NOUN njb-55 147 70     SPACE njb-55 147 71 1 1 NUM njb-55 147 72     SPACE njb-55 147 73 and and CCONJ njb-55 147 74     SPACE njb-55 147 75 6 6 NUM njb-55 147 76 , , PUNCT njb-55 147 77     SPACE njb-55 147 78 100bp 100bp PROPN njb-55 147 79     SPACE njb-55 147 80 gene gene NOUN njb-55 147 81     SPACE njb-55 147 82 ruler ruler NOUN njb-55 147 83 . . PUNCT njb-55 147 84         SPACE njb-55 148 1 gel gel NOUN njb-55 148 2     SPACE njb-55 148 3 run run NOUN njb-55 148 4       SPACE njb-55 148 5 performed perform VERB njb-55 148 6     SPACE njb-55 148 7 using use VERB njb-55 148 8     SPACE njb-55 148 9 2 2 NUM njb-55 148 10 % % NOUN njb-55 148 11     SPACE njb-55 148 12 agarose agarose NOUN njb-55 148 13 . . PUNCT njb-55 148 14     SPACE njb-55 149 1 concentration concentration NOUN njb-55 149 2     SPACE njb-55 149 3 of of ADP njb-55 149 4     SPACE njb-55 149 5 1 1 NUM njb-55 149 6 mm mm NOUN njb-55 149 7 . . PUNCT njb-55 149 8     SPACE njb-55 150 1 this this DET njb-55 150 2     SPACE njb-55 150 3 assay assay NOUN njb-55 150 4     SPACE njb-55 150 5 proved prove VERB njb-55 150 6     SPACE njb-55 150 7 to to PART njb-55 150 8     SPACE njb-55 150 9 be be AUX njb-55 150 10     SPACE njb-55 150 11 only only ADV njb-55 150 12     SPACE njb-55 150 13 specific specific ADJ njb-55 150 14     SPACE njb-55 150 15 to to ADP njb-55 150 16     SPACE njb-55 150 17 l. l. PROPN njb-55 150 18     SPACE njb-55 150 19 javanica javanica PROPN njb-55 150 20     SPACE njb-55 150 21 serovar serovar NOUN njb-55 150 22     SPACE njb-55 150 23 out out ADP njb-55 150 24     SPACE njb-55 150 25 of of ADP njb-55 150 26     SPACE njb-55 150 27 nine nine NUM njb-55 150 28     SPACE njb-55 150 29 serovars serovar NOUN njb-55 150 30     SPACE njb-55 150 31 tested test VERB njb-55 150 32     SPACE njb-55 150 33 ( ( PUNCT njb-55 150 34 fig fig NOUN njb-55 150 35 . . PUNCT njb-55 150 36     SPACE njb-55 150 37 3b 3b NUM njb-55 150 38 ) ) PUNCT njb-55 150 39 . . PUNCT njb-55 150 40     SPACE njb-55 151 1 the the DET njb-55 151 2     SPACE njb-55 151 3 pcr pcr NOUN njb-55 151 4     SPACE njb-55 151 5 product product NOUN njb-55 151 6     SPACE njb-55 151 7 of of ADP njb-55 151 8     SPACE njb-55 151 9 both both DET njb-55 151 10     SPACE njb-55 151 11 p28/29 p28/29 NOUN njb-55 151 12     SPACE njb-55 151 13 and and CCONJ njb-55 151 14     SPACE njb-55 151 15 p30/31 p30/31 PROPN njb-55 151 16     SPACE njb-55 151 17 of of ADP njb-55 151 18     SPACE njb-55 151 19 size size NOUN njb-55 151 20     SPACE njb-55 151 21 385bp 385bp PROPN njb-55 151 22     SPACE njb-55 151 23 and and CCONJ njb-55 151 24     SPACE njb-55 151 25 427bp 427bp NOUN njb-55 151 26     SPACE njb-55 151 27 respectively respectively ADV njb-55 151 28     SPACE njb-55 151 29 were be AUX njb-55 151 30     SPACE njb-55 151 31 then then ADV njb-55 151 32     SPACE njb-55 151 33 individually individually ADV njb-55 151 34     SPACE njb-55 151 35 cloned clone VERB njb-55 151 36     SPACE njb-55 151 37 into into ADP njb-55 151 38     SPACE njb-55 151 39 pgemt pgemt NOUN njb-55 151 40     SPACE njb-55 151 41 easy easy ADJ njb-55 151 42     SPACE njb-55 151 43 ta ta AUX njb-55 151 44     SPACE njb-55 151 45 cloning clone VERB njb-55 151 46     SPACE njb-55 151 47 vector vector NOUN njb-55 151 48     SPACE njb-55 151 49 and and CCONJ njb-55 151 50     SPACE njb-55 151 51 transformed transform VERB njb-55 151 52     SPACE njb-55 151 53 to to ADP njb-55 151 54     SPACE njb-55 151 55 jm109 jm109 PROPN njb-55 151 56     SPACE njb-55 151 57 e. e. PROPN njb-55 151 58     SPACE njb-55 151 59 coli coli PROPN njb-55 151 60     SPACE njb-55 151 61 competent competent ADJ njb-55 151 62     SPACE njb-55 151 63 cells cell NOUN njb-55 151 64     SPACE njb-55 151 65 and and CCONJ njb-55 151 66     SPACE njb-55 151 67 plated plate VERB njb-55 151 68     SPACE njb-55 151 69 on on ADP njb-55 151 70     SPACE njb-55 151 71 lb lb NOUN njb-55 151 72     SPACE njb-55 151 73 ampicillin ampicillin NOUN njb-55 151 74     SPACE njb-55 151 75 plates plate NOUN njb-55 151 76     SPACE njb-55 151 77 with with ADP njb-55 151 78     SPACE njb-55 151 79 iptg iptg NOUN njb-55 151 80     SPACE njb-55 151 81 and and CCONJ njb-55 151 82     SPACE njb-55 151 83 x‐gal x‐gal PROPN njb-55 151 84     SPACE njb-55 151 85 ( ( PUNCT njb-55 151 86 fig fig NOUN njb-55 151 87 . . PUNCT njb-55 151 88     SPACE njb-55 151 89 4 4 NUM njb-55 151 90 ) ) PUNCT njb-55 151 91 . . PUNCT njb-55 151 92     SPACE njb-55 152 1 the the DET njb-55 152 2     SPACE njb-55 152 3 discrete discrete ADJ njb-55 152 4     SPACE njb-55 152 5 five five NUM njb-55 152 6     SPACE njb-55 152 7 white white ADJ njb-55 152 8     SPACE njb-55 152 9 colonies colony NOUN njb-55 152 10     SPACE njb-55 152 11 and and CCONJ njb-55 152 12     SPACE njb-55 152 13 a a DET njb-55 152 14     SPACE njb-55 152 15 blue blue ADJ njb-55 152 16     SPACE njb-55 152 17 colony colony NOUN njb-55 152 18     SPACE njb-55 152 19 were be AUX njb-55 152 20     SPACE njb-55 152 21 then then ADV njb-55 152 22     SPACE njb-55 152 23 picked pick VERB njb-55 152 24 , , PUNCT njb-55 152 25     SPACE njb-55 152 26 cultured culture VERB njb-55 152 27     SPACE njb-55 152 28 and and CCONJ njb-55 152 29     SPACE njb-55 152 30 plasmid plasmid PROPN njb-55 152 31     SPACE njb-55 152 32 was be AUX njb-55 152 33     SPACE njb-55 152 34 isolated isolate VERB njb-55 152 35 . . PUNCT njb-55 152 36     SPACE njb-55 153 1 the the DET njb-55 153 2     SPACE njb-55 153 3 recombinants recombinant NOUN njb-55 153 4     SPACE njb-55 153 5 were be AUX njb-55 153 6     SPACE njb-55 153 7 then then ADV njb-55 153 8     SPACE njb-55 153 9 characterized characterize VERB njb-55 153 10     SPACE njb-55 153 11 by by ADP njb-55 153 12     SPACE njb-55 153 13 releasing release VERB njb-55 153 14     SPACE njb-55 153 15 the the DET njb-55 153 16     SPACE njb-55 153 17 insert insert NOUN njb-55 153 18     SPACE njb-55 153 19 ( ( PUNCT njb-55 153 20 fig fig NOUN njb-55 153 21 . . PUNCT njb-55 153 22     SPACE njb-55 154 1 5a 5a NUM njb-55 154 2     SPACE njb-55 154 3 and and CCONJ njb-55 154 4     SPACE njb-55 154 5 5b 5b NUM njb-55 154 6 ) ) PUNCT njb-55 154 7     SPACE njb-55 154 8 using use VERB njb-55 154 9     SPACE njb-55 154 10 ecori ecori PROPN njb-55 154 11     SPACE njb-55 154 12 restriction restriction NOUN njb-55 154 13     SPACE njb-55 154 14 enzyme enzyme NOUN njb-55 154 15     SPACE njb-55 154 16 followed follow VERB njb-55 154 17     SPACE njb-55 154 18 by by ADP njb-55 154 19     SPACE njb-55 154 20 pcr pcr PROPN njb-55 154 21     SPACE njb-55 154 22 reaction reaction NOUN njb-55 154 23     SPACE njb-55 154 24 ( ( PUNCT njb-55 154 25 fig fig NOUN njb-55 154 26 . . PUNCT njb-55 154 27     SPACE njb-55 154 28 6a 6a NOUN njb-55 154 29     SPACE njb-55 154 30 and and CCONJ njb-55 154 31     SPACE njb-55 154 32 6b 6b NUM njb-55 154 33 ) ) PUNCT njb-55 154 34     SPACE njb-55 154 35 for for ADP njb-55 154 36     SPACE njb-55 154 37 confirmation confirmation NOUN njb-55 154 38 . . PUNCT njb-55 154 39     SPACE njb-55 155 1 the the DET njb-55 155 2     SPACE njb-55 155 3 cloned cloned ADJ njb-55 155 4     SPACE njb-55 155 5 sequences sequence NOUN njb-55 155 6     SPACE njb-55 155 7 were be AUX njb-55 155 8     SPACE njb-55 155 9 then then ADV njb-55 155 10     SPACE njb-55 155 11 sent send VERB njb-55 155 12     SPACE njb-55 155 13 for for ADP njb-55 155 14     SPACE njb-55 155 15 sequence sequence NOUN njb-55 155 16     SPACE njb-55 155 17 analysis analysis NOUN njb-55 155 18     SPACE njb-55 155 19 ( ( PUNCT njb-55 155 20 mwg mwg PROPN njb-55 155 21     SPACE njb-55 155 22 biotech biotech PROPN njb-55 155 23     SPACE njb-55 155 24 pvt pvt PROPN njb-55 155 25 . . PUNCT njb-55 155 26     SPACE njb-55 155 27 ltd ltd PROPN njb-55 155 28 . . PROPN njb-55 155 29 , , PUNCT njb-55 155 30     SPACE njb-55 155 31 bangalore bangalore PROPN njb-55 155 32 ) ) PUNCT njb-55 155 33     SPACE njb-55 155 34 in in ADP njb-55 155 35     SPACE njb-55 155 36 order order NOUN njb-55 155 37     SPACE njb-55 155 38 to to PART njb-55 155 39     SPACE njb-55 155 40 facilitate facilitate VERB njb-55 155 41     SPACE njb-55 155 42 further further ADJ njb-55 155 43     SPACE njb-55 155 44 studies study NOUN njb-55 155 45 . . PUNCT njb-55 155 46     SPACE njb-55 155 47 one one NOUN njb-55 155 48     SPACE njb-55 155 49 of of ADP njb-55 155 50     SPACE njb-55 155 51 the the DET njb-55 155 52     SPACE njb-55 155 53 main main ADJ njb-55 155 54     SPACE njb-55 155 55 advantages advantage NOUN njb-55 155 56     SPACE njb-55 155 57 of of ADP njb-55 155 58     SPACE njb-55 155 59 using use VERB njb-55 155 60     SPACE njb-55 155 61 this this DET njb-55 155 62     SPACE njb-55 155 63 technique technique NOUN njb-55 155 64     SPACE njb-55 155 65 on on ADP njb-55 155 66     SPACE njb-55 155 67 various various ADJ njb-55 155 68     SPACE njb-55 155 69 biological biological ADJ njb-55 155 70     SPACE njb-55 155 71 samples sample NOUN njb-55 155 72     SPACE njb-55 155 73 is be AUX njb-55 155 74         SPACE njb-55 155 75 possibility possibility NOUN njb-55 155 76     SPACE njb-55 155 77 to to PART njb-55 155 78     SPACE njb-55 155 79 detect detect VERB njb-55 155 80     SPACE njb-55 155 81 pathogenic pathogenic ADJ njb-55 155 82     SPACE njb-55 155 83 leptospires leptospire NOUN njb-55 155 84     SPACE njb-55 155 85 first first ADV njb-55 155 86     SPACE njb-55 155 87 by by ADP njb-55 155 88     SPACE njb-55 155 89 pcr pcr PROPN njb-55 155 90     SPACE njb-55 155 91 assay assay NOUN njb-55 155 92     SPACE njb-55 155 93 and and CCONJ njb-55 155 94     SPACE njb-55 155 95 subsequently subsequently ADV njb-55 155 96     SPACE njb-55 155 97 confirming confirm VERB njb-55 155 98     SPACE njb-55 155 99 it it PRON njb-55 155 100     SPACE njb-55 155 101 through through ADP njb-55 155 102     SPACE njb-55 155 103 re re NOUN njb-55 155 104     SPACE njb-55 155 105 digestion digestion NOUN njb-55 155 106     SPACE njb-55 155 107 thus thus ADV njb-55 155 108     SPACE njb-55 155 109 improving improve VERB njb-55 155 110     SPACE njb-55 155 111 the the DET njb-55 155 112     SPACE njb-55 155 113 specificity specificity NOUN njb-55 155 114     SPACE njb-55 155 115 of of ADP njb-55 155 116     SPACE njb-55 155 117 the the DET njb-55 155 118     SPACE njb-55 155 119 test test NOUN njb-55 155 120 . . PUNCT njb-55 155 121     SPACE njb-55 156 1 further further ADJ njb-55 156 2     SPACE njb-55 156 3 sequence sequence NOUN njb-55 156 4     SPACE njb-55 156 5 analysis analysis NOUN njb-55 156 6     SPACE njb-55 156 7 may may AUX njb-55 156 8     SPACE njb-55 156 9 reveal reveal VERB njb-55 156 10     SPACE njb-55 156 11 the the DET njb-55 156 12     SPACE njb-55 156 13 associated associate VERB njb-55 156 14     SPACE njb-55 156 15 serovar serovar NOUN njb-55 156 16     SPACE njb-55 156 17 causing cause VERB njb-55 156 18     SPACE njb-55 156 19 the the DET njb-55 156 20     SPACE njb-55 156 21 disease disease NOUN njb-55 156 22 . . PUNCT njb-55 156 23     SPACE njb-55 157 1 the the DET njb-55 157 2     SPACE njb-55 157 3 direct direct ADJ njb-55 157 4     SPACE njb-55 157 5 method method NOUN njb-55 157 6     SPACE njb-55 157 7 of of ADP njb-55 157 8     SPACE njb-55 157 9 diagnosis diagnosis NOUN njb-55 157 10     SPACE njb-55 157 11 of of ADP njb-55 157 12     SPACE njb-55 157 13 leptospirosis leptospirosis NOUN njb-55 157 14     SPACE njb-55 157 15 is be AUX njb-55 157 16     SPACE njb-55 157 17 by by ADP njb-55 157 18     SPACE njb-55 157 19 nepal nepal ADJ njb-55 157 20     SPACE njb-55 157 21 journal journal NOUN njb-55 157 22     SPACE njb-55 157 23 of of ADP njb-55 157 24     SPACE njb-55 157 25 biotechnology biotechnology NOUN njb-55 157 26 . . PUNCT njb-55 157 27     SPACE njb-55 158 1 jan jan PROPN njb-55 158 2 . . PROPN njb-55 158 3     SPACE njb-55 158 4 2011 2011 NUM njb-55 158 5 , , PUNCT njb-55 158 6     SPACE njb-55 158 7 vol vol NOUN njb-55 158 8 . . PUNCT njb-55 158 9     SPACE njb-55 158 10 1 1 NUM njb-55 158 11 , , PUNCT njb-55 158 12     SPACE njb-55 158 13 no no INTJ njb-55 158 14 . . PUNCT njb-55 158 15     SPACE njb-55 158 16 1 1 NUM njb-55 158 17 : : PUNCT njb-55 158 18     SPACE njb-55 158 19 22‐30 22‐30 NUM njb-55 158 20     SPACE njb-55 158 21 28 28 NUM njb-55 158 22     SPACE njb-55 158 23 biotechnology biotechnology NOUN njb-55 158 24     SPACE njb-55 158 25 society society NOUN njb-55 158 26     SPACE njb-55 158 27 of of ADP njb-55 158 28     SPACE njb-55 158 29 nepal nepal NOUN njb-55 158 30     SPACE njb-55 158 31 ( ( PUNCT njb-55 158 32 bsn bsn PROPN njb-55 158 33 ) ) PUNCT njb-55 158 34 , , PUNCT njb-55 158 35     SPACE njb-55 158 36 all all DET njb-55 158 37     SPACE njb-55 158 38 rights right NOUN njb-55 158 39     SPACE njb-55 158 40 reserved reserve VERB njb-55 158 41       SPACE njb-55 158 42 culture culture NOUN njb-55 158 43     SPACE njb-55 158 44 isolation isolation NOUN njb-55 158 45 , , PUNCT njb-55 158 46     SPACE njb-55 158 47 but but CCONJ njb-55 158 48     SPACE njb-55 158 49 it it PRON njb-55 158 50     SPACE njb-55 158 51 is be AUX njb-55 158 52     SPACE njb-55 158 53 time time NOUN njb-55 158 54     SPACE njb-55 158 55 consuming consuming NOUN njb-55 158 56     SPACE njb-55 158 57 and and CCONJ njb-55 158 58     SPACE njb-55 158 59 potentially potentially ADV njb-55 158 60     SPACE njb-55 158 61 biohazardous biohazardous ADJ njb-55 158 62 . . PUNCT njb-55 158 63     SPACE njb-55 158 64 another another DET njb-55 158 65     SPACE njb-55 158 66 traditional traditional ADJ njb-55 158 67     SPACE njb-55 158 68 method method NOUN njb-55 158 69     SPACE njb-55 158 70 is be AUX njb-55 158 71     SPACE njb-55 158 72 the the DET njb-55 158 73     SPACE njb-55 158 74 detection detection NOUN njb-55 158 75     SPACE njb-55 158 76 of of ADP njb-55 158 77     SPACE njb-55 158 78 antibodies antibody NOUN njb-55 158 79     SPACE njb-55 158 80 ( ( PUNCT njb-55 158 81 serological serological ADJ njb-55 158 82     SPACE njb-55 158 83 tests test NOUN njb-55 158 84 ) ) PUNCT njb-55 158 85     SPACE njb-55 158 86 but but CCONJ njb-55 158 87     SPACE njb-55 158 88 it it PRON njb-55 158 89     SPACE njb-55 158 90 is be AUX njb-55 158 91     SPACE njb-55 158 92 also also ADV njb-55 158 93     SPACE njb-55 158 94 time time NOUN njb-55 158 95     SPACE njb-55 158 96 consuming consuming NOUN njb-55 158 97     SPACE njb-55 158 98 and and CCONJ njb-55 158 99     SPACE njb-55 158 100 fails fail VERB njb-55 158 101     SPACE njb-55 158 102 to to PART njb-55 158 103     SPACE njb-55 158 104 identify identify VERB njb-55 158 105     SPACE njb-55 158 106 the the DET njb-55 158 107     SPACE njb-55 158 108 infecting infect VERB njb-55 158 109     SPACE njb-55 158 110 serovar serovar NOUN njb-55 158 111 . . PUNCT njb-55 158 112     SPACE njb-55 158 113 to to PART njb-55 158 114     SPACE njb-55 158 115 overcome overcome VERB njb-55 158 116     SPACE njb-55 158 117 the the DET njb-55 158 118     SPACE njb-55 158 119 limitations limitation NOUN njb-55 158 120     SPACE njb-55 158 121 of of ADP njb-55 158 122     SPACE njb-55 158 123 cultivation cultivation NOUN njb-55 158 124     SPACE njb-55 158 125 and and CCONJ njb-55 158 126     SPACE njb-55 158 127 serology serology NOUN njb-55 158 128 , , PUNCT njb-55 158 129     SPACE njb-55 158 130 we we PRON njb-55 158 131     SPACE njb-55 158 132 have have AUX njb-55 158 133     SPACE njb-55 158 134 used use VERB njb-55 158 135     SPACE njb-55 158 136 pcr pcr DET njb-55 158 137     SPACE njb-55 158 138 amplification amplification NOUN njb-55 158 139     SPACE njb-55 158 140 of of ADP njb-55 158 141     SPACE njb-55 158 142 leptospiral leptospiral ADJ njb-55 158 143     SPACE njb-55 158 144 dna dna NOUN njb-55 158 145     SPACE njb-55 158 146 for for ADP njb-55 158 147     SPACE njb-55 158 148 the the DET njb-55 158 149     SPACE njb-55 158 150 diagnosis diagnosis NOUN njb-55 158 151     SPACE njb-55 158 152 of of ADP njb-55 158 153     SPACE njb-55 158 154 leptospirosis leptospirosis NOUN njb-55 158 155     SPACE njb-55 158 156 at at ADP njb-55 158 157     SPACE njb-55 158 158 an an DET njb-55 158 159     SPACE njb-55 158 160 early early ADJ njb-55 158 161     SPACE njb-55 158 162 stage stage NOUN njb-55 158 163     SPACE njb-55 158 164 of of ADP njb-55 158 165     SPACE njb-55 158 166 illness illness NOUN njb-55 158 167 . . PUNCT njb-55 158 168     SPACE njb-55 159 1 in in ADP njb-55 159 2     SPACE njb-55 159 3 a a DET njb-55 159 4     SPACE njb-55 159 5 previous previous ADJ njb-55 159 6     SPACE njb-55 159 7 study study NOUN njb-55 159 8     SPACE njb-55 159 9 by by ADP njb-55 159 10     SPACE njb-55 159 11 ( ( PUNCT njb-55 159 12 merien merien PROPN njb-55 159 13     SPACE njb-55 159 14 et et PROPN njb-55 159 15     SPACE njb-55 159 16 al al PROPN njb-55 159 17 . . PROPN njb-55 159 18 , , PUNCT njb-55 159 19     SPACE njb-55 159 20 1992 1992 NUM njb-55 159 21     SPACE njb-55 159 22 and and CCONJ njb-55 159 23     SPACE njb-55 159 24 gravekamp gravekamp PROPN njb-55 159 25     SPACE njb-55 159 26 et et PROPN njb-55 159 27     SPACE njb-55 159 28 al al PROPN njb-55 159 29 , , PUNCT njb-55 159 30 . . PUNCT njb-55 159 31     SPACE njb-55 159 32 1993 1993 NUM njb-55 159 33 ) ) PUNCT njb-55 159 34 , , PUNCT njb-55 159 35     SPACE njb-55 159 36 the the DET njb-55 159 37     SPACE njb-55 159 38 16s 16s NUM njb-55 159 39     SPACE njb-55 159 40 rrna rrna PROPN njb-55 159 41     SPACE njb-55 159 42 gene gene NOUN njb-55 159 43     SPACE njb-55 159 44 target target NOUN njb-55 159 45     SPACE njb-55 159 46 could could AUX njb-55 159 47     SPACE njb-55 159 48 not not PART njb-55 159 49     SPACE njb-55 159 50 differentiate differentiate VERB njb-55 159 51     SPACE njb-55 159 52 pathogenic pathogenic ADJ njb-55 159 53     SPACE njb-55 159 54 and and CCONJ njb-55 159 55     SPACE njb-55 159 56 nonpathogenic nonpathogenic PROPN njb-55 159 57     SPACE njb-55 159 58 leptospira leptospira PROPN njb-55 159 59 . . PUNCT njb-55 159 60         SPACE njb-55 160 1 the the DET njb-55 160 2     SPACE njb-55 160 3 development development NOUN njb-55 160 4     SPACE njb-55 160 5 of of ADP njb-55 160 6     SPACE njb-55 160 7 pcr pcr PROPN njb-55 160 8     SPACE njb-55 160 9 assay assay NOUN njb-55 160 10     SPACE njb-55 160 11 targeting target VERB njb-55 160 12     SPACE njb-55 160 13 partial partial ADJ njb-55 160 14     SPACE njb-55 160 15 sequence sequence NOUN njb-55 160 16     SPACE njb-55 160 17 of of ADP njb-55 160 18     SPACE njb-55 160 19 lipl21 lipl21 NOUN njb-55 160 20     SPACE njb-55 160 21 and and CCONJ njb-55 160 22     SPACE njb-55 160 23 lipl41 lipl41 PROPN njb-55 160 24     SPACE njb-55 160 25 gene gene NOUN njb-55 160 26     SPACE njb-55 160 27 of of ADP njb-55 160 28     SPACE njb-55 160 29 pathogenic pathogenic ADJ njb-55 160 30     SPACE njb-55 160 31 leptospires leptospire NOUN njb-55 160 32     SPACE njb-55 160 33 using use VERB njb-55 160 34     SPACE njb-55 160 35 in‐house in‐house PROPN njb-55 160 36     SPACE njb-55 160 37 designed design VERB njb-55 160 38     SPACE njb-55 160 39 p28/29 p28/29 NOUN njb-55 160 40     SPACE njb-55 160 41 and and CCONJ njb-55 160 42     SPACE njb-55 160 43 p30/31 p30/31 ADJ njb-55 160 44     SPACE njb-55 160 45 primers primer NOUN njb-55 160 46 , , PUNCT njb-55 160 47     SPACE njb-55 160 48 with with ADP njb-55 160 49     SPACE njb-55 160 50 a a DET njb-55 160 51     SPACE njb-55 160 52 product product NOUN njb-55 160 53     SPACE njb-55 160 54 size size NOUN njb-55 160 55     SPACE njb-55 160 56 of of ADP njb-55 160 57     SPACE njb-55 160 58 385bp 385bp PROPN njb-55 160 59     SPACE njb-55 160 60 and and CCONJ njb-55 160 61     SPACE njb-55 160 62 427bp 427bp PROPN njb-55 160 63 , , PUNCT njb-55 160 64     SPACE njb-55 160 65 respectively respectively ADV njb-55 160 66 . . PUNCT njb-55 160 67     SPACE njb-55 161 1 these these DET njb-55 161 2     SPACE njb-55 161 3 primers primer NOUN njb-55 161 4     SPACE njb-55 161 5 were be AUX njb-55 161 6     SPACE njb-55 161 7 designed design VERB njb-55 161 8     SPACE njb-55 161 9 by by ADP njb-55 161 10     SPACE njb-55 161 11 using use VERB njb-55 161 12     SPACE njb-55 161 13 gene gene NOUN njb-55 161 14     SPACE njb-55 161 15 tool tool NOUN njb-55 161 16     SPACE njb-55 161 17 lite lite NOUN njb-55 161 18     SPACE njb-55 161 19 software software NOUN njb-55 161 20 . . PUNCT njb-55 161 21     SPACE njb-55 162 1 the the DET njb-55 162 2     SPACE njb-55 162 3 gene gene NOUN njb-55 162 4     SPACE njb-55 162 5 sequence sequence NOUN njb-55 162 6     SPACE njb-55 162 7 coding coding NOUN njb-55 162 8     SPACE njb-55 162 9 for for ADP njb-55 162 10     SPACE njb-55 162 11 the the DET njb-55 162 12     SPACE njb-55 162 13 respective respective ADJ njb-55 162 14     SPACE njb-55 162 15 surface surface NOUN njb-55 162 16     SPACE njb-55 162 17 protein protein NOUN njb-55 162 18     SPACE njb-55 162 19 was be AUX njb-55 162 20     SPACE njb-55 162 21 subjected subject VERB njb-55 162 22     SPACE njb-55 162 23 to to ADP njb-55 162 24     SPACE njb-55 162 25 nucleotide nucleotide NOUN njb-55 162 26     SPACE njb-55 162 27 blast blast NOUN njb-55 162 28     SPACE njb-55 162 29 to to PART njb-55 162 30     SPACE njb-55 162 31 get get AUX njb-55 162 32     SPACE njb-55 162 33 conserved conserve VERB njb-55 162 34     SPACE njb-55 162 35 sequences sequence NOUN njb-55 162 36 . . PUNCT njb-55 162 37     SPACE njb-55 163 1 the the DET njb-55 163 2     SPACE njb-55 163 3 most most ADJ njb-55 163 4     SPACE njb-55 163 5 conserved conserve VERB njb-55 163 6     SPACE njb-55 163 7 sequence sequence NOUN njb-55 163 8     SPACE njb-55 163 9 was be AUX njb-55 163 10     SPACE njb-55 163 11 then then ADV njb-55 163 12     SPACE njb-55 163 13 chosen choose VERB njb-55 163 14     SPACE njb-55 163 15 and and CCONJ njb-55 163 16     SPACE njb-55 163 17 primers primer NOUN njb-55 163 18     SPACE njb-55 163 19 were be AUX njb-55 163 20     SPACE njb-55 163 21 designed design VERB njb-55 163 22 . . PUNCT njb-55 163 23     SPACE njb-55 164 1 the the DET njb-55 164 2     SPACE njb-55 164 3 newly newly ADV njb-55 164 4     SPACE njb-55 164 5 developed develop VERB njb-55 164 6     SPACE njb-55 164 7 pcr pcr NOUN njb-55 164 8     SPACE njb-55 164 9 using use VERB njb-55 164 10     SPACE njb-55 164 11 in‐house in‐house PROPN njb-55 164 12     SPACE njb-55 164 13 designed design VERB njb-55 164 14     SPACE njb-55 164 15 primers primer NOUN njb-55 164 16     SPACE njb-55 164 17 produces produce VERB njb-55 164 18     SPACE njb-55 164 19 expected expect VERB njb-55 164 20         SPACE njb-55 164 21 amplicons amplicon NOUN njb-55 164 22     SPACE njb-55 164 23 sizes size NOUN njb-55 164 24     SPACE njb-55 164 25 i.e. i.e. X njb-55 164 26     SPACE njb-55 164 27 385bp 385bp PROPN njb-55 164 28     SPACE njb-55 164 29 for for ADP njb-55 164 30     SPACE njb-55 164 31 lipl21 lipl21 NOUN njb-55 164 32     SPACE njb-55 164 33 and and CCONJ njb-55 164 34     SPACE njb-55 164 35 427bp 427bp PROPN njb-55 164 36     SPACE njb-55 164 37 for for ADP njb-55 164 38     SPACE njb-55 164 39 lipl41 lipl41 PROPN njb-55 164 40 . . PUNCT njb-55 164 41       SPACE njb-55 165 1 afterwards afterwards ADV njb-55 165 2     SPACE njb-55 165 3 when when SCONJ njb-55 165 4     SPACE njb-55 165 5 this this DET njb-55 165 6     SPACE njb-55 165 7 pcr pcr PROPN njb-55 165 8     SPACE njb-55 165 9 protocol protocol NOUN njb-55 165 10     SPACE njb-55 165 11 was be AUX njb-55 165 12     SPACE njb-55 165 13 applied apply VERB njb-55 165 14     SPACE njb-55 165 15 to to PART njb-55 165 16     SPACE njb-55 165 17 examine examine VERB njb-55 165 18     SPACE njb-55 165 19 all all DET njb-55 165 20     SPACE njb-55 165 21 the the DET njb-55 165 22     SPACE njb-55 165 23 test test NOUN njb-55 165 24     SPACE njb-55 165 25 samples sample NOUN njb-55 165 26     SPACE njb-55 165 27 of of ADP njb-55 165 28     SPACE njb-55 165 29 this this DET njb-55 165 30     SPACE njb-55 165 31 species specie NOUN njb-55 165 32     SPACE njb-55 165 33 also also ADV njb-55 165 34     SPACE njb-55 165 35 produces produce VERB njb-55 165 36     SPACE njb-55 165 37 specific specific ADJ njb-55 165 38     SPACE njb-55 165 39 amplicons amplicon NOUN njb-55 165 40 . . PUNCT njb-55 165 41       SPACE njb-55 166 1 no no DET njb-55 166 2     SPACE njb-55 166 3 amplicons amplicon NOUN njb-55 166 4     SPACE njb-55 166 5 of of ADP njb-55 166 6     SPACE njb-55 166 7 specific specific ADJ njb-55 166 8     SPACE njb-55 166 9 size size NOUN njb-55 166 10     SPACE njb-55 166 11 was be AUX njb-55 166 12     SPACE njb-55 166 13 observed observe VERB njb-55 166 14     SPACE njb-55 166 15 in in ADP njb-55 166 16     SPACE njb-55 166 17 case case NOUN njb-55 166 18     SPACE njb-55 166 19 of of ADP njb-55 166 20     SPACE njb-55 166 21 non‐target non‐target PROPN njb-55 166 22     SPACE njb-55 166 23 leptospira leptospira PROPN njb-55 166 24 , , PUNCT njb-55 166 25     SPACE njb-55 166 26 or or CCONJ njb-55 166 27     SPACE njb-55 166 28 other other ADJ njb-55 166 29     SPACE njb-55 166 30 bacterial bacterial ADJ njb-55 166 31     SPACE njb-55 166 32 figure figure NOUN njb-55 166 33     SPACE njb-55 166 34 3 3 NUM njb-55 166 35 . . PUNCT njb-55 166 36     SPACE njb-55 166 37 ( ( PUNCT njb-55 166 38 a a X njb-55 166 39 ) ) PUNCT njb-55 166 40     SPACE njb-55 166 41 the the DET njb-55 166 42     SPACE njb-55 166 43 specificity specificity NOUN njb-55 166 44     SPACE njb-55 166 45 of of ADP njb-55 166 46     SPACE njb-55 166 47 this this DET njb-55 166 48     SPACE njb-55 166 49 primer primer NOUN njb-55 166 50     SPACE njb-55 166 51 tested test VERB njb-55 166 52     SPACE njb-55 166 53 on on ADP njb-55 166 54     SPACE njb-55 166 55 nine nine NUM njb-55 166 56     SPACE njb-55 166 57 different different ADJ njb-55 166 58     SPACE njb-55 166 59 serovars serovar NOUN njb-55 166 60     SPACE njb-55 166 61 under under ADP njb-55 166 62     SPACE njb-55 166 63 optimized optimize VERB njb-55 166 64     SPACE njb-55 166 65 conditions condition NOUN njb-55 166 66     SPACE njb-55 166 67 for for ADP njb-55 166 68     SPACE njb-55 166 69 lipl21 lipl21 NOUN njb-55 166 70 . . PUNCT njb-55 166 71       SPACE njb-55 167 1 lane lane NOUN njb-55 167 2     SPACE njb-55 167 3 3‐11 3‐11 NUM njb-55 167 4     SPACE njb-55 167 5 different different ADJ njb-55 167 6     SPACE njb-55 167 7 serovars serovar NOUN njb-55 167 8 , , PUNCT njb-55 167 9     SPACE njb-55 167 10 lane lane NOUN njb-55 167 11     SPACE njb-55 167 12 2 2 NUM njb-55 167 13 , , PUNCT njb-55 167 14     SPACE njb-55 167 15 nega‐ nega‐ PROPN njb-55 167 16 tive tive ADJ njb-55 167 17     SPACE njb-55 167 18 control control NOUN njb-55 167 19     SPACE njb-55 167 20 and and CCONJ njb-55 167 21     SPACE njb-55 167 22 lane lane NOUN njb-55 167 23     SPACE njb-55 167 24 1 1 NUM njb-55 167 25 , , PUNCT njb-55 167 26     SPACE njb-55 167 27 100bp 100bp PROPN njb-55 167 28     SPACE njb-55 167 29 gene gene NOUN njb-55 167 30     SPACE njb-55 167 31 ruler ruler NOUN njb-55 167 32 . . PUNCT njb-55 167 33     SPACE njb-55 168 1 gel gel NOUN njb-55 168 2     SPACE njb-55 168 3 run run VERB njb-55 168 4     SPACE njb-55 168 5 performed perform VERB njb-55 168 6     SPACE njb-55 168 7 using use VERB njb-55 168 8     SPACE njb-55 168 9 2.5 2.5 NUM njb-55 168 10 % % NOUN njb-55 168 11     SPACE njb-55 168 12 agarose agarose NOUN njb-55 168 13 . . PUNCT njb-55 168 14     SPACE njb-55 169 1 figure figure VERB njb-55 169 2     SPACE njb-55 169 3 3 3 NUM njb-55 169 4 . . PUNCT njb-55 169 5     SPACE njb-55 169 6 ( ( PUNCT njb-55 169 7 b b X njb-55 169 8 ) ) PUNCT njb-55 169 9     SPACE njb-55 169 10 the the DET njb-55 169 11     SPACE njb-55 169 12 specificity specificity NOUN njb-55 169 13     SPACE njb-55 169 14 of of ADP njb-55 169 15     SPACE njb-55 169 16 this this DET njb-55 169 17     SPACE njb-55 169 18 primer primer NOUN njb-55 169 19     SPACE njb-55 169 20 tested test VERB njb-55 169 21     SPACE njb-55 169 22 on on ADP njb-55 169 23     SPACE njb-55 169 24 nine nine NUM njb-55 169 25     SPACE njb-55 169 26 different different ADJ njb-55 169 27     SPACE njb-55 169 28 serovars serovar NOUN njb-55 169 29     SPACE njb-55 169 30 under under ADP njb-55 169 31     SPACE njb-55 169 32 optimized optimize VERB njb-55 169 33     SPACE njb-55 169 34 conditions condition NOUN njb-55 169 35     SPACE njb-55 169 36 for for ADP njb-55 169 37     SPACE njb-55 169 38 lipl41 lipl41 PROPN njb-55 169 39 . . PUNCT njb-55 169 40       SPACE njb-55 170 1 lane lane NOUN njb-55 170 2     SPACE njb-55 170 3 2‐5 2‐5 NUM njb-55 170 4     SPACE njb-55 170 5 different different ADJ njb-55 170 6     SPACE njb-55 170 7 serovars serovar NOUN njb-55 170 8     SPACE njb-55 170 9 and and CCONJ njb-55 170 10     SPACE njb-55 170 11 lane lane NOUN njb-55 170 12     SPACE njb-55 170 13 1and 1and NUM njb-55 170 14     SPACE njb-55 170 15 6 6 NUM njb-55 170 16 , , PUNCT njb-55 170 17     SPACE njb-55 170 18 100bp 100bp PROPN njb-55 170 19     SPACE njb-55 170 20 gene gene NOUN njb-55 170 21     SPACE njb-55 170 22 ruler ruler NOUN njb-55 170 23 . . PUNCT njb-55 170 24       SPACE njb-55 171 1 gel gel NOUN njb-55 171 2     SPACE njb-55 171 3 run run NOUN njb-55 171 4     SPACE njb-55 171 5 performed perform VERB njb-55 171 6     SPACE njb-55 171 7 using use VERB njb-55 171 8     SPACE njb-55 171 9 2.5 2.5 NUM njb-55 171 10 % % NOUN njb-55 171 11     SPACE njb-55 171 12 agarose agarose NOUN njb-55 171 13 . . PUNCT njb-55 171 14     SPACE njb-55 172 1 figure figure VERB njb-55 172 2     SPACE njb-55 172 3 4 4 NUM njb-55 172 4 . . PUNCT njb-55 172 5         SPACE njb-55 172 6 plate plate NOUN njb-55 172 7     SPACE njb-55 172 8 showing show VERB njb-55 172 9     SPACE njb-55 172 10 recombinant recombinant ADJ njb-55 172 11     SPACE njb-55 172 12 colonies colony NOUN njb-55 172 13     SPACE njb-55 172 14 nepal nepal ADJ njb-55 172 15     SPACE njb-55 172 16 journal journal NOUN njb-55 172 17     SPACE njb-55 172 18 of of ADP njb-55 172 19     SPACE njb-55 172 20 biotechnology biotechnology NOUN njb-55 172 21 . . PUNCT njb-55 172 22     SPACE njb-55 173 1 jan jan PROPN njb-55 173 2 . . PROPN njb-55 173 3     SPACE njb-55 173 4 2011 2011 NUM njb-55 173 5 , , PUNCT njb-55 173 6     SPACE njb-55 173 7 vol vol NOUN njb-55 173 8 . . PUNCT njb-55 173 9     SPACE njb-55 173 10 1 1 NUM njb-55 173 11 , , PUNCT njb-55 173 12     SPACE njb-55 173 13 no no INTJ njb-55 173 14 . . PUNCT njb-55 173 15     SPACE njb-55 173 16 1 1 NUM njb-55 173 17 : : PUNCT njb-55 173 18     SPACE njb-55 173 19 22‐30 22‐30 NUM njb-55 173 20     SPACE njb-55 173 21 29 29 NUM njb-55 173 22     SPACE njb-55 173 23 biotechnology biotechnology NOUN njb-55 173 24     SPACE njb-55 173 25 society society NOUN njb-55 173 26     SPACE njb-55 173 27 of of ADP njb-55 173 28     SPACE njb-55 173 29 nepal nepal NOUN njb-55 173 30     SPACE njb-55 173 31 ( ( PUNCT njb-55 173 32 bsn bsn PROPN njb-55 173 33 ) ) PUNCT njb-55 173 34 , , PUNCT njb-55 173 35     SPACE njb-55 173 36 all all DET njb-55 173 37     SPACE njb-55 173 38 rights right NOUN njb-55 173 39     SPACE njb-55 173 40 reserved reserve VERB njb-55 173 41       SPACE njb-55 173 42 species specie NOUN njb-55 173 43 . . PUNCT njb-55 173 44       SPACE njb-55 174 1 besides besides SCONJ njb-55 174 2     SPACE njb-55 174 3 non‐specific non‐specific PROPN njb-55 174 4     SPACE njb-55 174 5 amplicon amplicon NOUN njb-55 174 6     SPACE njb-55 174 7 of of ADP njb-55 174 8     SPACE njb-55 174 9 different different ADJ njb-55 174 10     SPACE njb-55 174 11 size size NOUN njb-55 174 12     SPACE njb-55 174 13 did do AUX njb-55 174 14     SPACE njb-55 174 15 not not PART njb-55 174 16     SPACE njb-55 174 17 appear appear VERB njb-55 174 18     SPACE njb-55 174 19 in in ADP njb-55 174 20     SPACE njb-55 174 21 any any DET njb-55 174 22     SPACE njb-55 174 23 case case NOUN njb-55 174 24 . . PUNCT njb-55 174 25     SPACE njb-55 175 1 in in ADP njb-55 175 2     SPACE njb-55 175 3 conclusion conclusion NOUN njb-55 175 4 , , PUNCT njb-55 175 5     SPACE njb-55 175 6 the the DET njb-55 175 7     SPACE njb-55 175 8 assay assay NOUN njb-55 175 9     SPACE njb-55 175 10 targeting target VERB njb-55 175 11     SPACE njb-55 175 12 partial partial ADJ njb-55 175 13     SPACE njb-55 175 14 sequence sequence NOUN njb-55 175 15     SPACE njb-55 175 16 of of ADP njb-55 175 17     SPACE njb-55 175 18 lipl41 lipl41 PROPN njb-55 175 19     SPACE njb-55 175 20 gene gene NOUN njb-55 175 21     SPACE njb-55 175 22 showed show VERB njb-55 175 23     SPACE njb-55 175 24 high high ADJ njb-55 175 25     SPACE njb-55 175 26 sensitivity sensitivity NOUN njb-55 175 27     SPACE njb-55 175 28 and and CCONJ njb-55 175 29     SPACE njb-55 175 30 specificity specificity NOUN njb-55 175 31     SPACE njb-55 175 32 for for ADP njb-55 175 33     SPACE njb-55 175 34 l. l. PROPN njb-55 175 35     SPACE njb-55 175 36 javanica javanica PROPN njb-55 175 37     SPACE njb-55 175 38 out out ADP njb-55 175 39     SPACE njb-55 175 40 of of ADP njb-55 175 41     SPACE njb-55 175 42 9 9 NUM njb-55 175 43     SPACE njb-55 175 44 leptospires leptospire NOUN njb-55 175 45     SPACE njb-55 175 46 tested test VERB njb-55 175 47 . . PUNCT njb-55 175 48     SPACE njb-55 176 1 the the DET njb-55 176 2     SPACE njb-55 176 3 pcr pcr PROPN njb-55 176 4     SPACE njb-55 176 5 assay assay NOUN njb-55 176 6     SPACE njb-55 176 7 targeting target VERB njb-55 176 8     SPACE njb-55 176 9 partial partial ADJ njb-55 176 10     SPACE njb-55 176 11 sequence sequence NOUN njb-55 176 12     SPACE njb-55 176 13 of of ADP njb-55 176 14     SPACE njb-55 176 15 lipl21 lipl21 NOUN njb-55 176 16     SPACE njb-55 176 17 gene gene NOUN njb-55 176 18 , , PUNCT njb-55 176 19     SPACE njb-55 176 20 which which PRON njb-55 176 21     SPACE njb-55 176 22 showed show VERB njb-55 176 23     SPACE njb-55 176 24 good good ADJ njb-55 176 25     SPACE njb-55 176 26 sensitivity sensitivity NOUN njb-55 176 27     SPACE njb-55 176 28 and and CCONJ njb-55 176 29     SPACE njb-55 176 30 specificity specificity NOUN njb-55 176 31     SPACE njb-55 176 32 with with ADP njb-55 176 33     SPACE njb-55 176 34 all all DET njb-55 176 35     SPACE njb-55 176 36 the the DET njb-55 176 37     SPACE njb-55 176 38 nine nine NUM njb-55 176 39     SPACE njb-55 176 40 serovars serovar NOUN njb-55 176 41     SPACE njb-55 176 42 tested test VERB njb-55 176 43     SPACE njb-55 176 44 and and CCONJ njb-55 176 45     SPACE njb-55 176 46 may may AUX njb-55 176 47     SPACE njb-55 176 48 also also ADV njb-55 176 49     SPACE njb-55 176 50 likely likely ADJ njb-55 176 51     SPACE njb-55 176 52 to to PART njb-55 176 53     SPACE njb-55 176 54 detect detect VERB njb-55 176 55     SPACE njb-55 176 56 other other ADJ njb-55 176 57     SPACE njb-55 176 58 pathogenic pathogenic ADJ njb-55 176 59     SPACE njb-55 176 60 serovars serovar NOUN njb-55 176 61     SPACE njb-55 176 62 as as SCONJ njb-55 176 63     SPACE njb-55 176 64 the the DET njb-55 176 65     SPACE njb-55 176 66 lipl21 lipl21 NOUN njb-55 176 67     SPACE njb-55 176 68 sequence sequence NOUN njb-55 176 69     SPACE njb-55 176 70 targeted target VERB njb-55 176 71     SPACE njb-55 176 72 under under ADP njb-55 176 73     SPACE njb-55 176 74 the the DET njb-55 176 75     SPACE njb-55 176 76 assay assay NOUN njb-55 176 77     SPACE njb-55 176 78 is be AUX njb-55 176 79     SPACE njb-55 176 80 conserved conserve VERB njb-55 176 81     SPACE njb-55 176 82 among among ADP njb-55 176 83     SPACE njb-55 176 84 all all DET njb-55 176 85     SPACE njb-55 176 86 the the DET njb-55 176 87     SPACE njb-55 176 88 pathogenic pathogenic ADJ njb-55 176 89     SPACE njb-55 176 90 leptospires leptospire NOUN njb-55 176 91     SPACE njb-55 176 92 hence hence ADV njb-55 176 93     SPACE njb-55 176 94 may may AUX njb-55 176 95     SPACE njb-55 176 96 be be AUX njb-55 176 97     SPACE njb-55 176 98 valuable valuable ADJ njb-55 176 99     SPACE njb-55 176 100 tool tool NOUN njb-55 176 101     SPACE njb-55 176 102 for for ADP njb-55 176 103     SPACE njb-55 176 104 early early ADJ njb-55 176 105     SPACE njb-55 176 106 diagnosis diagnosis NOUN njb-55 176 107     SPACE njb-55 176 108 of of ADP njb-55 176 109     SPACE njb-55 176 110 pathogenic pathogenic ADJ njb-55 176 111     SPACE njb-55 176 112 leptospires leptospire NOUN njb-55 176 113     SPACE njb-55 176 114 directly directly ADV njb-55 176 115     SPACE njb-55 176 116 from from ADP njb-55 176 117     SPACE njb-55 176 118 biological biological ADJ njb-55 176 119     SPACE njb-55 176 120 samples sample NOUN njb-55 176 121     SPACE njb-55 176 122 with with ADP njb-55 176 123     SPACE njb-55 176 124 clinical clinical ADJ njb-55 176 125     SPACE njb-55 176 126 suspicion suspicion NOUN njb-55 176 127     SPACE njb-55 176 128 of of ADP njb-55 176 129     SPACE njb-55 176 130 leptospirosis leptospirosis NOUN njb-55 176 131 . . PUNCT njb-55 176 132     SPACE njb-55 177 1 figure figure NOUN njb-55 177 2     SPACE njb-55 177 3 5 5 NUM njb-55 177 4 . . PUNCT njb-55 177 5     SPACE njb-55 177 6 ( ( PUNCT njb-55 177 7 a a X njb-55 177 8 ) ) PUNCT njb-55 177 9     SPACE njb-55 177 10 insert insert ADJ njb-55 177 11     SPACE njb-55 177 12 release release NOUN njb-55 177 13     SPACE njb-55 177 14 of of ADP njb-55 177 15     SPACE njb-55 177 16 lipl21from lipl21from NOUN njb-55 177 17     SPACE njb-55 177 18 recombinant recombinant NOUN njb-55 177 19     SPACE njb-55 177 20 pgem‐te pgem‐te NOUN njb-55 177 21     SPACE njb-55 177 22 vector vector NOUN njb-55 177 23 . . PUNCT njb-55 177 24         SPACE njb-55 178 1 gel gel NOUN njb-55 178 2     SPACE njb-55 178 3 run run NOUN njb-55 178 4     SPACE njb-55 178 5 performed perform VERB njb-55 178 6     SPACE njb-55 178 7 using use VERB njb-55 178 8     SPACE njb-55 178 9 2.5 2.5 NUM njb-55 178 10 % % NOUN njb-55 178 11     SPACE njb-55 178 12 agarose agarose NOUN njb-55 178 13 . . PUNCT njb-55 178 14     SPACE njb-55 179 1 figure figure VERB njb-55 179 2     SPACE njb-55 179 3 5 5 NUM njb-55 179 4 . . PUNCT njb-55 179 5     SPACE njb-55 179 6 ( ( PUNCT njb-55 179 7 b b X njb-55 179 8 ) ) PUNCT njb-55 179 9     SPACE njb-55 179 10 insert insert ADJ njb-55 179 11     SPACE njb-55 179 12 release release NOUN njb-55 179 13     SPACE njb-55 179 14 of of ADP njb-55 179 15     SPACE njb-55 179 16 lipl41 lipl41 PROPN njb-55 179 17     SPACE njb-55 179 18 from from ADP njb-55 179 19     SPACE njb-55 179 20 recombi‐ recombi‐ ADJ njb-55 179 21 nant nant ADJ njb-55 179 22     SPACE njb-55 179 23 pgem‐te pgem‐te NOUN njb-55 179 24     SPACE njb-55 179 25 vector vector NOUN njb-55 179 26 . . PUNCT njb-55 179 27       SPACE njb-55 180 1 gel gel NOUN njb-55 180 2     SPACE njb-55 180 3 run run NOUN njb-55 180 4     SPACE njb-55 180 5 performed perform VERB njb-55 180 6     SPACE njb-55 180 7 using use VERB njb-55 180 8     SPACE njb-55 180 9 2.5 2.5 NUM njb-55 180 10 % % NOUN njb-55 180 11     SPACE njb-55 180 12 agarose agarose NOUN njb-55 180 13 . . PUNCT njb-55 180 14     SPACE njb-55 181 1 figure figure VERB njb-55 181 2     SPACE njb-55 181 3 6 6 NUM njb-55 181 4 . . PUNCT njb-55 181 5     SPACE njb-55 181 6 ( ( PUNCT njb-55 181 7 a a X njb-55 181 8 ) ) PUNCT njb-55 181 9     SPACE njb-55 181 10 pcr pcr NOUN njb-55 181 11     SPACE njb-55 181 12 for for ADP njb-55 181 13     SPACE njb-55 181 14 lipl21 lipl21 NOUN njb-55 181 15     SPACE njb-55 181 16 gene gene NOUN njb-55 181 17     SPACE njb-55 181 18 for for ADP njb-55 181 19     SPACE njb-55 181 20 confirmation confirmation NOUN njb-55 181 21 . . PUNCT njb-55 181 22       SPACE njb-55 182 1 gel gel NOUN njb-55 182 2     SPACE njb-55 182 3 run run NOUN njb-55 182 4     SPACE njb-55 182 5 performed perform VERB njb-55 182 6     SPACE njb-55 182 7 using use VERB njb-55 182 8     SPACE njb-55 182 9 2.5 2.5 NUM njb-55 182 10 % % NOUN njb-55 182 11     SPACE njb-55 182 12 agarose agarose NOUN njb-55 182 13 . . PUNCT njb-55 182 14     SPACE njb-55 183 1 figure figure VERB njb-55 183 2     SPACE njb-55 183 3 6 6 NUM njb-55 183 4 . . PUNCT njb-55 183 5     SPACE njb-55 183 6 ( ( PUNCT njb-55 183 7 b b X njb-55 183 8 ) ) PUNCT njb-55 183 9     SPACE njb-55 183 10 pcr pcr NOUN njb-55 183 11     SPACE njb-55 183 12 for for ADP njb-55 183 13     SPACE njb-55 183 14 lipl41 lipl41 PROPN njb-55 183 15     SPACE njb-55 183 16 gene gene NOUN njb-55 183 17     SPACE njb-55 183 18 for for ADP njb-55 183 19     SPACE njb-55 183 20 confirmation confirmation NOUN njb-55 183 21 . . PUNCT njb-55 183 22       SPACE njb-55 184 1 gel gel NOUN njb-55 184 2     SPACE njb-55 184 3 run run NOUN njb-55 184 4     SPACE njb-55 184 5 performed perform VERB njb-55 184 6     SPACE njb-55 184 7 using use VERB njb-55 184 8     SPACE njb-55 184 9 2.5 2.5 NUM njb-55 184 10 % % NOUN njb-55 184 11     SPACE njb-55 184 12 agarose agarose NOUN njb-55 184 13 . . PUNCT njb-55 184 14     SPACE njb-55 185 1 references reference NOUN njb-55 185 2     SPACE njb-55 185 3 abdollahpour abdollahpour PROPN njb-55 185 4 , , PUNCT njb-55 185 5     SPACE njb-55 185 6 g g NOUN njb-55 185 7 : : PUNCT njb-55 185 8     SPACE njb-55 185 9 a a DET njb-55 185 10     SPACE njb-55 185 11 review review NOUN njb-55 185 12     SPACE njb-55 185 13 on on ADP njb-55 185 14     SPACE njb-55 185 15 leptospirosis leptospirosis NOUN njb-55 185 16 . . PUNCT njb-55 185 17       SPACE njb-55 186 1 proceedings proceeding NOUN njb-55 186 2     SPACE njb-55 186 3 of of ADP njb-55 186 4     SPACE njb-55 186 5 leptospirosis leptospirosis NOUN njb-55 186 6     SPACE njb-55 186 7 research research NOUN njb-55 186 8             SPACE njb-55 186 9 conference conference NOUN njb-55 186 10 , , PUNCT njb-55 186 11     SPACE njb-55 186 12 japan japan PROPN njb-55 186 13 , , PUNCT njb-55 186 14     SPACE njb-55 186 15 matsoyamam matsoyamam PROPN njb-55 186 16     SPACE njb-55 186 17 ; ; PUNCT njb-55 186 18 1990 1990 NUM njb-55 186 19 : : PUNCT njb-55 186 20     SPACE njb-55 186 21 34‐37 34‐37 NUM njb-55 186 22 . . PUNCT njb-55 186 23     SPACE njb-55 186 24 bharti bharti NOUN njb-55 186 25 , , PUNCT njb-55 186 26     SPACE njb-55 186 27 ar ar ADJ njb-55 186 28 , , PUNCT njb-55 186 29     SPACE njb-55 186 30 nally nally ADV njb-55 186 31     SPACE njb-55 186 32 je je PROPN njb-55 186 33 , , PUNCT njb-55 186 34     SPACE njb-55 186 35 ricaldi ricaldi PROPN njb-55 186 36     SPACE njb-55 186 37 jn jn PROPN njb-55 186 38 , , PUNCT njb-55 186 39     SPACE njb-55 186 40 matthias matthias PROPN njb-55 186 41     SPACE njb-55 186 42 ma ma PROPN njb-55 186 43 , , PUNCT njb-55 186 44     SPACE njb-55 187 1 diaz diaz PROPN njb-55 187 2     SPACE njb-55 187 3 mm mm PROPN njb-55 187 4 : : PUNCT njb-55 187 5     SPACE njb-55 187 6 leptospirosis leptospirosis NOUN njb-55 187 7 : : PUNCT njb-55 187 8     SPACE njb-55 187 9 a a DET njb-55 187 10     SPACE njb-55 187 11 zoonotic zoonotic NOUN njb-55 187 12     SPACE njb-55 187 13 disease disease NOUN njb-55 187 14     SPACE njb-55 187 15 of of ADP njb-55 187 16     SPACE njb-55 187 17 global global ADJ njb-55 187 18     SPACE njb-55 187 19 importance importance NOUN njb-55 187 20 . . PUNCT njb-55 187 21     SPACE njb-55 188 1 lancet lancet NOUN njb-55 188 2     SPACE njb-55 188 3 infect infect VERB njb-55 188 4     SPACE njb-55 188 5 dis dis PROPN njb-55 188 6     SPACE njb-55 188 7 2003 2003 NUM njb-55 188 8 , , PUNCT njb-55 188 9     SPACE njb-55 188 10 3:757–771 3:757–771 NUM njb-55 188 11 . . PUNCT njb-55 188 12       SPACE njb-55 189 1 gravekamph gravekamph PROPN njb-55 189 2     SPACE njb-55 189 3 c c PROPN njb-55 189 4 , , PUNCT njb-55 189 5     SPACE njb-55 189 6 kemp kemp PROPN njb-55 189 7     SPACE njb-55 189 8 vad vad PROPN njb-55 189 9 , , PUNCT njb-55 189 10     SPACE njb-55 189 11 franzend franzend PROPN njb-55 189 12     SPACE njb-55 189 13 m m PROPN njb-55 189 14 , , PUNCT njb-55 189 15     SPACE njb-55 189 16 carrington carrington PROPN njb-55 189 17     SPACE njb-55 189 18 d d PROPN njb-55 189 19 , , PUNCT njb-55 189 20         SPACE njb-55 189 21 schoone schoone PROPN njb-55 189 22     SPACE njb-55 189 23 gj gj PROPN njb-55 189 24 , , PUNCT njb-55 189 25       SPACE njb-55 189 26 eys ey NOUN njb-55 189 27     SPACE njb-55 189 28 gjjmv gjjmv NOUN njb-55 189 29 , , PUNCT njb-55 189 30       SPACE njb-55 189 31 everardr everardr ADV njb-55 189 32     SPACE njb-55 189 33 c0r c0r PROPN njb-55 189 34 , , PUNCT njb-55 189 35       SPACE njb-55 189 36 hartskeeraln hartskeeraln PROPN njb-55 189 37     SPACE njb-55 189 38 ra ra PROPN njb-55 189 39 , , PUNCT njb-55 189 40     SPACE njb-55 189 41 terpstra terpstra PROPN njb-55 189 42     SPACE njb-55 189 43 wj wj PROPN njb-55 189 44 : : PUNCT njb-55 189 45         SPACE njb-55 189 46 detection detection NOUN njb-55 189 47     SPACE njb-55 189 48 of of ADP njb-55 189 49     SPACE njb-55 189 50 seven seven NUM njb-55 189 51     SPACE njb-55 189 52 species specie NOUN njb-55 189 53     SPACE njb-55 189 54 of of ADP njb-55 189 55     SPACE njb-55 189 56 pathogenic pathogenic ADJ njb-55 189 57     SPACE njb-55 189 58 leptospires leptospire NOUN njb-55 189 59     SPACE njb-55 189 60 by by ADP njb-55 189 61     SPACE njb-55 189 62 pcr pcr NOUN njb-55 189 63     SPACE njb-55 189 64 using use VERB njb-55 189 65     SPACE njb-55 189 66 two two NUM njb-55 189 67     SPACE njb-55 189 68 sets set NOUN njb-55 189 69     SPACE njb-55 189 70 of of ADP njb-55 189 71     SPACE njb-55 189 72 nepal nepal ADJ njb-55 189 73     SPACE njb-55 189 74 journal journal NOUN njb-55 189 75     SPACE njb-55 189 76 of of ADP njb-55 189 77     SPACE njb-55 189 78 biotechnology biotechnology NOUN njb-55 189 79 . . PUNCT njb-55 189 80     SPACE njb-55 190 1 jan jan PROPN njb-55 190 2 . . PROPN njb-55 190 3     SPACE njb-55 190 4 2011 2011 NUM njb-55 190 5 , , PUNCT njb-55 190 6     SPACE njb-55 190 7 vol vol NOUN njb-55 190 8 . . PUNCT njb-55 190 9     SPACE njb-55 190 10 1 1 NUM njb-55 190 11 , , PUNCT njb-55 190 12     SPACE njb-55 190 13 no no INTJ njb-55 190 14 . . PUNCT njb-55 190 15     SPACE njb-55 190 16 1 1 NUM njb-55 190 17 : : PUNCT njb-55 190 18     SPACE njb-55 190 19 22‐30 22‐30 NUM njb-55 190 20     SPACE njb-55 190 21 30 30 NUM njb-55 190 22     SPACE njb-55 190 23 biotechnology biotechnology NOUN njb-55 190 24     SPACE njb-55 190 25 society society NOUN njb-55 190 26     SPACE njb-55 190 27 of of ADP njb-55 190 28     SPACE njb-55 190 29 nepal nepal NOUN njb-55 190 30     SPACE njb-55 190 31 ( ( PUNCT njb-55 190 32 bsn bsn PROPN njb-55 190 33 ) ) PUNCT njb-55 190 34 , , PUNCT njb-55 190 35     SPACE njb-55 190 36 all all DET njb-55 190 37     SPACE njb-55 190 38 rights right NOUN njb-55 190 39     SPACE njb-55 190 40 reserved reserve VERB njb-55 190 41       SPACE njb-55 190 42 primers primer NOUN njb-55 190 43 . . PUNCT njb-55 190 44       SPACE njb-55 191 1 j j NOUN njb-55 191 2     SPACE njb-55 191 3 of of ADP njb-55 191 4     SPACE njb-55 191 5 gen gen PROPN njb-55 191 6     SPACE njb-55 191 7 microbiol microbiol PROPN njb-55 191 8       SPACE njb-55 191 9 1993 1993 NUM njb-55 191 10 , , PUNCT njb-55 191 11     SPACE njb-55 191 12 139 139 NUM njb-55 191 13 : : PUNCT njb-55 191 14     SPACE njb-55 191 15 1691‐1700 1691‐1700 NUM njb-55 191 16 . . PUNCT njb-55 191 17     SPACE njb-55 192 1 hoshino hoshino NOUN njb-55 192 2     SPACE njb-55 192 3 k k PROPN njb-55 192 4 , , PUNCT njb-55 192 5     SPACE njb-55 192 6 yamasaki yamasaki PROPN njb-55 192 7     SPACE njb-55 192 8 s s PROPN njb-55 192 9 , , PUNCT njb-55 192 10     SPACE njb-55 192 11 mukhopadhyaym mukhopadhyaym PROPN njb-55 192 12 , , PUNCT njb-55 192 13     SPACE njb-55 192 14 ak ak PROPN njb-55 192 15 , , PUNCT njb-55 192 16     SPACE njb-55 192 17 chakraborty chakraborty PROPN njb-55 192 18     SPACE njb-55 192 19 s s PROPN njb-55 192 20 , , PUNCT njb-55 192 21     SPACE njb-55 192 22 basu basu PROPN njb-55 192 23     SPACE njb-55 192 24 a a DET njb-55 192 25 , , PUNCT njb-55 192 26     SPACE njb-55 192 27 bhattacharya bhattacharya VERB njb-55 192 28     SPACE njb-55 192 29 sk sk NOUN njb-55 192 30 , , PUNCT njb-55 192 31     SPACE njb-55 192 32 nair nair NOUN njb-55 192 33 , , PUNCT njb-55 192 34     SPACE njb-55 192 35 gb gb PROPN njb-55 192 36 , , PUNCT njb-55 192 37     SPACE njb-55 192 38 shimada shimada PROPN njb-55 192 39     SPACE njb-55 192 40 t t PROPN njb-55 192 41 : : PUNCT njb-55 192 42     SPACE njb-55 192 43 development development NOUN njb-55 192 44     SPACE njb-55 192 45 and and CCONJ njb-55 192 46     SPACE njb-55 192 47 evaluation evaluation NOUN njb-55 192 48     SPACE njb-55 192 49 of of ADP njb-55 192 50     SPACE njb-55 192 51 a a DET njb-55 192 52     SPACE njb-55 192 53 multiplex multiplex ADJ njb-55 192 54     SPACE njb-55 192 55 pcr pcr PROPN njb-55 192 56     SPACE njb-55 192 57 assay assay NOUN njb-55 192 58     SPACE njb-55 192 59 for for ADP njb-55 192 60     SPACE njb-55 192 61 rapid rapid ADJ njb-55 192 62     SPACE njb-55 192 63 detection detection NOUN njb-55 192 64     SPACE njb-55 192 65 of of ADP njb-55 192 66     SPACE njb-55 192 67 toxigenic toxigenic ADJ njb-55 192 68     SPACE njb-55 192 69 vibrio vibrio PROPN njb-55 192 70     SPACE njb-55 192 71 cholerae cholerae PROPN njb-55 192 72     SPACE njb-55 192 73 o1 o1 PROPN njb-55 192 74     SPACE njb-55 192 75 and and CCONJ njb-55 192 76     SPACE njb-55 192 77 o139 o139 PROPN njb-55 192 78 . . PUNCT njb-55 192 79         SPACE njb-55 193 1 fems fem VERB njb-55 193 2     SPACE njb-55 193 3 immunol immunol NOUN njb-55 193 4     SPACE njb-55 193 5 med me VERB njb-55 193 6     SPACE njb-55 193 7 microbiol microbiol PROPN njb-55 193 8     SPACE njb-55 193 9 1998 1998 NUM njb-55 193 10 , , PUNCT njb-55 193 11     SPACE njb-55 193 12 20 20 NUM njb-55 193 13 : : PUNCT njb-55 193 14     SPACE njb-55 193 15 201–207 201–207 NUM njb-55 193 16 . . PUNCT njb-55 193 17       SPACE njb-55 193 18 levett levett PROPN njb-55 193 19 , , PUNCT njb-55 193 20     SPACE njb-55 193 21 pn pn PROPN njb-55 193 22 , , PUNCT njb-55 193 23     SPACE njb-55 193 24 songee songee PROPN njb-55 193 25     SPACE njb-55 193 26 l l NOUN njb-55 193 27 , , PUNCT njb-55 193 28     SPACE njb-55 193 29 branch branch NOUN njb-55 193 30 , , PUNCT njb-55 193 31     SPACE njb-55 193 32 carol carol NOUN njb-55 193 33     SPACE njb-55 193 34 u u NOUN njb-55 193 35 , , PUNCT njb-55 193 36     SPACE njb-55 193 37 whittington whittington PROPN njb-55 193 38 , , PUNCT njb-55 193 39     SPACE njb-55 193 40 charles charle VERB njb-55 193 41     SPACE njb-55 193 42 n n PROPN njb-55 193 43 , , PUNCT njb-55 193 44     SPACE njb-55 193 45 edwards edwards PROPN njb-55 193 46 , , PUNCT njb-55 193 47         SPACE njb-55 193 48 paxton paxton PROPN njb-55 193 49     SPACE njb-55 193 50 h h PROPN njb-55 193 51 : : PUNCT njb-55 193 52         SPACE njb-55 193 53 two two NUM njb-55 193 54     SPACE njb-55 193 55 methods method NOUN njb-55 193 56     SPACE njb-55 193 57 for for ADP njb-55 193 58     SPACE njb-55 193 59 rapid rapid ADJ njb-55 193 60     SPACE njb-55 193 61 serological serological ADJ njb-55 193 62     SPACE njb-55 193 63 diagnosis diagnosis NOUN njb-55 193 64     SPACE njb-55 193 65 of of ADP njb-55 193 66     SPACE njb-55 193 67 acute acute ADJ njb-55 193 68     SPACE njb-55 193 69 leptospirosis leptospirosis NOUN njb-55 193 70 . . PUNCT njb-55 193 71     SPACE njb-55 194 1 clin clin PROPN njb-55 194 2     SPACE njb-55 194 3 and and CCONJ njb-55 194 4     SPACE njb-55 194 5 diag diag NOUN njb-55 194 6 . . PUNCT njb-55 194 7     SPACE njb-55 194 8 lab lab NOUN njb-55 194 9     SPACE njb-55 194 10 immunology immunology NOUN njb-55 194 11     SPACE njb-55 194 12 2001 2001 NUM njb-55 194 13 , , PUNCT njb-55 194 14     SPACE njb-55 194 15 8(2):349‐351 8(2):349‐351 NUM njb-55 194 16 . . PUNCT njb-55 194 17     SPACE njb-55 195 1 levett levett PROPN njb-55 195 2     SPACE njb-55 195 3 pn pn PROPN njb-55 195 4 , , PUNCT njb-55 195 5     SPACE njb-55 195 6 morey morey PROPN njb-55 195 7     SPACE njb-55 195 8 re re NOUN njb-55 195 9 , , PUNCT njb-55 195 10     SPACE njb-55 195 11 galloway galloway NOUN njb-55 195 12     SPACE njb-55 195 13 rl rl PROPN njb-55 195 14 , , PUNCT njb-55 195 15     SPACE njb-55 195 16 turner turner PROPN njb-55 195 17     SPACE njb-55 195 18 de de PROPN njb-55 195 19 , , PUNCT njb-55 195 20     SPACE njb-55 195 21 steigerwalt steigerwalt PROPN njb-55 195 22     SPACE njb-55 195 23 ag ag PROPN njb-55 195 24 , , PUNCT njb-55 195 25     SPACE njb-55 195 26 mayer mayer NOUN njb-55 195 27     SPACE njb-55 195 28 lw lw PROPN njb-55 195 29 : : PUNCT njb-55 195 30     SPACE njb-55 195 31 detection detection NOUN njb-55 195 32     SPACE njb-55 195 33 of of ADP njb-55 195 34     SPACE njb-55 195 35 pathogenic pathogenic ADJ njb-55 195 36     SPACE njb-55 195 37 leptospires leptospire NOUN njb-55 195 38     SPACE njb-55 195 39 by by ADP njb-55 195 40     SPACE njb-55 195 41 real‐time real‐time NOUN njb-55 195 42     SPACE njb-55 195 43 quantitative quantitative ADJ njb-55 195 44     SPACE njb-55 195 45 pcr pcr NOUN njb-55 195 46 . . PUNCT njb-55 195 47         SPACE njb-55 196 1 j j PROPN njb-55 196 2     SPACE njb-55 196 3 med med ADJ njb-55 196 4     SPACE njb-55 196 5 microbiol microbiol PROPN njb-55 196 6     SPACE njb-55 196 7 2005 2005 NUM njb-55 196 8     SPACE njb-55 196 9 54(1):45‐49 54(1):45‐49 NUM njb-55 196 10 . . PUNCT njb-55 196 11     SPACE njb-55 197 1 merien merien PROPN njb-55 197 2     SPACE njb-55 197 3 f f PROPN njb-55 197 4 , , PUNCT njb-55 197 5     SPACE njb-55 197 6 amouriaux amouriaux PROPN njb-55 197 7     SPACE njb-55 197 8 p p PROPN njb-55 197 9 , , PUNCT njb-55 197 10     SPACE njb-55 198 1 perolat perolat PROPN njb-55 198 2     SPACE njb-55 198 3 p p PROPN njb-55 198 4 , , PUNCT njb-55 198 5     SPACE njb-55 198 6 baranton baranton PROPN njb-55 198 7     SPACE njb-55 198 8 g g NOUN njb-55 198 9 , , PUNCT njb-55 198 10     SPACE njb-55 198 11 saint saint NOUN njb-55 198 12     SPACE njb-55 198 13 girons giron NOUN njb-55 198 14     SPACE njb-55 198 15 i i NOUN njb-55 198 16 : : PUNCT njb-55 198 17       SPACE njb-55 198 18 polymerase polymerase NOUN njb-55 198 19     SPACE njb-55 198 20 chain chain NOUN njb-55 198 21     SPACE njb-55 198 22 reaction reaction NOUN njb-55 198 23     SPACE njb-55 198 24 for for ADP njb-55 198 25     SPACE njb-55 198 26 detection detection NOUN njb-55 198 27     SPACE njb-55 198 28 of of ADP njb-55 198 29     SPACE njb-55 198 30 leptospira leptospira PROPN njb-55 198 31     SPACE njb-55 198 32 spp spp PROPN njb-55 198 33 . . PUNCT njb-55 198 34     SPACE njb-55 198 35 in in ADP njb-55 198 36     SPACE njb-55 198 37 clinical clinical ADJ njb-55 198 38     SPACE njb-55 198 39 samples sample NOUN njb-55 198 40 . . PUNCT njb-55 198 41     SPACE njb-55 199 1 j j PROPN njb-55 199 2     SPACE njb-55 199 3 clin clin PROPN njb-55 199 4     SPACE njb-55 199 5 microbiol microbiol PROPN njb-55 199 6     SPACE njb-55 199 7 1992 1992 NUM njb-55 199 8 , , PUNCT njb-55 199 9     SPACE njb-55 199 10 30(9):2219‐2224 30(9):2219‐2224 NUM njb-55 199 11 . . PUNCT njb-55 199 12     SPACE njb-55 200 1 marshall marshall PROPN njb-55 200 2         SPACE njb-55 200 3 oj oj PROPN njb-55 200 4 : : PUNCT njb-55 200 5     SPACE njb-55 200 6 perl perl NOUN njb-55 200 7     SPACE njb-55 200 8 primer primer NOUN njb-55 200 9 : : PUNCT njb-55 200 10     SPACE njb-55 200 11 cross‐platform cross‐platform NOUN njb-55 200 12 , , PUNCT njb-55 200 13     SPACE njb-55 200 14 graphical graphical PROPN njb-55 200 15     SPACE njb-55 200 16 primer primer NOUN njb-55 200 17     SPACE njb-55 200 18 design design NOUN njb-55 200 19     SPACE njb-55 200 20 for for ADP njb-55 200 21     SPACE njb-55 200 22 standard standard NOUN njb-55 200 23 , , PUNCT njb-55 200 24     SPACE njb-55 200 25 bisulphite bisulphite PROPN njb-55 200 26     SPACE njb-55 200 27 and and CCONJ njb-55 200 28     SPACE njb-55 200 29 real‐time real‐time NOUN njb-55 200 30     SPACE njb-55 200 31 pcr pcr NOUN njb-55 200 32 . . PUNCT njb-55 200 33     SPACE njb-55 200 34 bio bio PROPN njb-55 200 35     SPACE njb-55 200 36 inf inf PROPN njb-55 200 37     SPACE njb-55 200 38 applications application NOUN njb-55 200 39     SPACE njb-55 200 40 note note NOUN njb-55 200 41     SPACE njb-55 200 42 2004 2004 NUM njb-55 200 43 , , PUNCT njb-55 200 44     SPACE njb-55 200 45 20 20 NUM njb-55 200 46     SPACE njb-55 200 47 ( ( PUNCT njb-55 200 48 15 15 NUM njb-55 200 49 ): ): PUNCT njb-55 200 50     SPACE njb-55 200 51 2471 2471 NUM njb-55 200 52 – – PUNCT njb-55 200 53 2472 2472 NUM njb-55 200 54 . . PUNCT njb-55 200 55     SPACE njb-55 200 56 serres serre NOUN njb-55 200 57     SPACE njb-55 200 58 gd gd PROPN njb-55 200 59 , , PUNCT njb-55 200 60     SPACE njb-55 200 61 levesque levesque PROPN njb-55 200 62     SPACE njb-55 200 63 b b PROPN njb-55 200 64 , , PUNCT njb-55 200 65     SPACE njb-55 200 66 higgins higgin NOUN njb-55 200 67     SPACE njb-55 200 68 r r NOUN njb-55 200 69 , , PUNCT njb-55 200 70     SPACE njb-55 200 71 major major ADJ njb-55 200 72     SPACE njb-55 200 73 m m ADJ njb-55 200 74 , , PUNCT njb-55 200 75     SPACE njb-55 200 76 laliberre laliberre PROPN njb-55 200 77     SPACE njb-55 200 78 d d PROPN njb-55 200 79 , , PUNCT njb-55 200 80     SPACE njb-55 200 81 bouliance bouliance NOUN njb-55 200 82     SPACE njb-55 200 83 n n NOUN njb-55 200 84 , , PUNCT njb-55 200 85     SPACE njb-55 200 86 duval duval NOUN njb-55 200 87     SPACE njb-55 200 88 b b NOUN njb-55 200 89 : : PUNCT njb-55 200 90     SPACE njb-55 200 91 need need VERB njb-55 200 92     SPACE njb-55 200 93 for for ADP njb-55 200 94     SPACE njb-55 200 95 vaccination vaccination NOUN njb-55 200 96     SPACE njb-55 200 97 of of ADP njb-55 200 98     SPACE njb-55 200 99 sewer sewer NOUN njb-55 200 100     SPACE njb-55 200 101 workers worker NOUN njb-55 200 102     SPACE njb-55 200 103 against against ADP njb-55 200 104     SPACE njb-55 200 105 leptospirosis leptospirosis NOUN njb-55 200 106     SPACE njb-55 200 107 and and CCONJ njb-55 200 108     SPACE njb-55 200 109 hepatitis hepatitis NOUN njb-55 200 110     SPACE njb-55 200 111 a. a. PROPN njb-55 200 112       SPACE njb-55 200 113 occup occup PROPN njb-55 200 114     SPACE njb-55 200 115 and and CCONJ njb-55 200 116     SPACE njb-55 200 117 envt envt NOUN njb-55 200 118 . . PUNCT njb-55 200 119     SPACE njb-55 201 1 med me VERB njb-55 201 2     SPACE njb-55 201 3 1995,52 1995,52 NUM njb-55 201 4 : : PUNCT njb-55 201 5     SPACE njb-55 201 6 505‐507 505‐507 NUM njb-55 201 7 . . PUNCT njb-55 201 8     SPACE njb-55 202 1 woodward woodward NOUN njb-55 202 2         SPACE njb-55 202 3 m m VERB njb-55 202 4     SPACE njb-55 202 5 j j PROPN njb-55 202 6 , , PUNCT njb-55 202 7     SPACE njb-55 203 1 redstone redstone NOUN njb-55 203 2     SPACE njb-55 203 3 js js PROPN njb-55 203 4     SPACE njb-55 203 5 : : PUNCT njb-55 203 6     SPACE njb-55 203 7 differentiation differentiation NOUN njb-55 203 8     SPACE njb-55 203 9 of of ADP njb-55 203 10       SPACE njb-55 203 11 leptospira leptospira PROPN njb-55 203 12     SPACE njb-55 203 13 serovars serovar NOUN njb-55 203 14     SPACE njb-55 203 15 by by ADP njb-55 203 16     SPACE njb-55 203 17 polymerase polymerase NOUN njb-55 203 18     SPACE njb-55 203 19 chain chain NOUN njb-55 203 20     SPACE njb-55 203 21 reaction reaction NOUN njb-55 203 22     SPACE njb-55 203 23 and and CCONJ njb-55 203 24     SPACE njb-55 203 25 restriction restriction NOUN njb-55 203 26     SPACE njb-55 203 27 fragment fragment ADJ njb-55 203 28     SPACE njb-55 203 29 length length NOUN njb-55 203 30     SPACE njb-55 203 31 polymorphism polymorphism NOUN njb-55 203 32 . . PUNCT njb-55 203 33     SPACE njb-55 204 1 vet vet NOUN njb-55 204 2     SPACE njb-55 204 3 rec rec PROPN njb-55 204 4     SPACE njb-55 204 5 1993 1993 NUM njb-55 204 6 , , PUNCT njb-55 204 7     SPACE njb-55 204 8 132 132 NUM njb-55 204 9 : : PUNCT njb-55 204 10     SPACE njb-55 204 11 325‐326 325‐326 NUM njb-55 204 12 . . PUNCT njb-55 204 13     SPACE njb-55 205 1 yasuda yasuda PROPN njb-55 205 2     SPACE njb-55 205 3 ph ph NOUN njb-55 205 4 , , PUNCT njb-55 205 5     SPACE njb-55 205 6 arnold arnold PROPN njb-55 205 7     SPACE njb-55 205 8 g g PROPN njb-55 205 9 , , PUNCT njb-55 205 10     SPACE njb-55 205 11 steigerwalt steigerwalt PROPN njb-55 205 12 , , PUNCT njb-55 205 13     SPACE njb-55 205 14 katherine katherine PROPN njb-55 205 15     SPACE njb-55 205 16 r r PROPN njb-55 205 17 , , PUNCT njb-55 205 18     SPACE njb-55 205 19 sulzer sulzer PROPN njb-55 205 20 , , PUNCT njb-55 205 21     SPACE njb-55 206 1 arnold arnold PROPN njb-55 206 2     SPACE njb-55 206 3 f f PROPN njb-55 206 4 , , PUNCT njb-55 206 5     SPACE njb-55 206 6 kaufmann kaufmann PROPN njb-55 206 7 , , PUNCT njb-55 206 8     SPACE njb-55 206 9 faye faye NOUN njb-55 206 10     SPACE njb-55 206 11 rogers roger NOUN njb-55 206 12             SPACE njb-55 206 13 don don PROPN njb-55 206 14 , , PUNCT njb-55 206 15     SPACE njb-55 206 16 j j PROPN njb-55 206 17 , , PUNCT njb-55 206 18     SPACE njb-55 206 19 brenner brenner PROPN njb-55 206 20 : : PUNCT njb-55 206 21     SPACE njb-55 206 22 deoxyribonucleic deoxyribonucleic PROPN njb-55 206 23     SPACE njb-55 206 24 acid acid NOUN njb-55 206 25     SPACE njb-55 206 26 relatedness relatedness VERB njb-55 206 27     SPACE njb-55 206 28 between between ADP njb-55 206 29     SPACE njb-55 206 30 serogroups serogroup NOUN njb-55 206 31     SPACE njb-55 206 32 and and CCONJ njb-55 206 33     SPACE njb-55 206 34 serovars serovar NOUN njb-55 206 35     SPACE njb-55 206 36 in in ADP njb-55 206 37     SPACE njb-55 206 38 the the DET njb-55 206 39     SPACE njb-55 206 40 family family NOUN njb-55 206 41     SPACE njb-55 206 42 leptospiraceae leptospiraceae PROPN njb-55 206 43     SPACE njb-55 206 44 with with ADP njb-55 206 45     SPACE njb-55 206 46 proposals proposal NOUN njb-55 206 47     SPACE njb-55 206 48 for for ADP njb-55 206 49     SPACE njb-55 206 50 seven seven NUM njb-55 206 51     SPACE njb-55 206 52 new new ADJ njb-55 206 53     SPACE njb-55 206 54 leptospira leptospira PROPN njb-55 206 55     SPACE njb-55 206 56 species specie NOUN njb-55 206 57 . . PUNCT njb-55 206 58       SPACE njb-55 207 1 int int NOUN njb-55 207 2       SPACE njb-55 207 3 j j PROPN njb-55 207 4       SPACE njb-55 207 5 systc systc PROPN njb-55 207 6     SPACE njb-55 207 7 bact bact NOUN njb-55 207 8     SPACE njb-55 207 9 1987 1987 NUM njb-55 207 10 , , PUNCT njb-55 207 11     SPACE njb-55 207 12 37(4 37(4 NUM njb-55 207 13 ): ): PUNCT njb-55 207 14     SPACE njb-55 207 15 407 407 NUM njb-55 207 16 ‐415 ‐415 NOUN njb-55 207 17 . . PUNCT njb-55 207 18     SPACE njb-55 208 1 zhang zhang PROPN njb-55 208 2 , , PUNCT njb-55 208 3     SPACE njb-55 208 4 y. y. PROPN njb-55 208 5 , , PUNCT njb-55 208 6     SPACE njb-55 208 7 dai dai PROPN njb-55 208 8 , , PUNCT njb-55 208 9     SPACE njb-55 208 10 b. b. PROPN njb-55 208 11       SPACE njb-55 208 12 ( ( PUNCT njb-55 208 13 1992 1992 NUM njb-55 208 14 ) ) PUNCT njb-55 208 15     SPACE njb-55 208 16 detection detection NOUN njb-55 208 17     SPACE njb-55 208 18 of of ADP njb-55 208 19     SPACE njb-55 208 20 leptospiral leptospiral ADJ njb-55 208 21     SPACE njb-55 208 22 dna dna NOUN njb-55 208 23     SPACE njb-55 208 24 in in ADP njb-55 208 25     SPACE njb-55 208 26 the the DET njb-55 208 27     SPACE njb-55 208 28 serum serum NOUN njb-55 208 29     SPACE njb-55 208 30 of of ADP njb-55 208 31     SPACE njb-55 208 32 175 175 NUM njb-55 208 33     SPACE njb-55 208 34 patients patient NOUN njb-55 208 35     SPACE njb-55 208 36 with with ADP njb-55 208 37     SPACE njb-55 208 38 early early ADJ njb-55 208 39     SPACE njb-55 208 40 leptospirosis leptospirosis NOUN njb-55 208 41     SPACE njb-55 208 42 by by ADP njb-55 208 43     SPACE njb-55 208 44 polymerase polymerase NOUN njb-55 208 45     SPACE njb-55 208 46 chain chain NOUN njb-55 208 47     SPACE njb-55 208 48 reaction reaction NOUN njb-55 208 49 . . PUNCT njb-55 208 50       SPACE njb-55 209 1 hua hua PROPN njb-55 209 2     SPACE njb-55 209 3 xi xi PROPN njb-55 209 4     SPACE njb-55 209 5 yi yi PROPN njb-55 209 6     SPACE njb-55 209 7 ke ke PROPN njb-55 209 8     SPACE njb-55 209 9 da da PROPN njb-55 209 10     SPACE njb-55 209 11 xue xue PROPN njb-55 209 12     SPACE njb-55 209 13 xue xue PROPN njb-55 209 14     SPACE njb-55 209 15 bao bao PROPN njb-55 209 16     SPACE njb-55 209 17 23(3):256‐60 23(3):256‐60 NUM njb-55 209 18 . . PUNCT njb-55 209 19       SPACE