Nepal Journal of Biotechnology. D e c . 2 0 1 8 Vol. 6, No. 1: 33-38 ISSN 2091-1130 (Print)/ISSN 2467-9319 (online) ORIGINAL RESEARCH ARTICLE ©NJB, Biotechnology Society of Nepal 33 Nepjol.info/index.php/njb Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors Gyanendra Bikram Shah1, 2, Krishna Gurung1, Bharat Prasad Baral1, Ram Raja Gurung3, Balkrishna Bhattachan4 1Pokhara Bigyan Tatha Prabidhi Campus, Pokhara Nepal 2Central Blood Transfusion Service, Nepal Red Cross Society, Pokhara, Nepal 3Deurali-Janata Pharmaceutical Pvt. Ltd., Kathmandu, Nepal 4Siddhi Memorial Hospital, Bhaktapur, Nepal Abstract Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) infections lead to chronic diseases and are the most common causes of liver cirrhosis and cancer in developing countries like Nepal. The study is carried out to determine the seroprevalence of HBV and HCV by using a Rapid kit method and Elisa Method to find out its risk factors. The cross-sectional study was done among blood donating people from 16th August 2016 to 19th November 2016. Blood donors in Pokhara Valley were screened for anti-HCV antibodies, anti-HBV antibodies using third generation ELISA kits and automated ELISA Processor in serology laboratory at Central Blood Transfusion Service (CBTS) of Nepal Red Cross Society (NRCS) in Pokhara, Nepal. 1777 (87.2%) units were male blood donors and 260 (12.6%) units were female donors out of 2037 participants. Gender wise, the ratio between male and female was 1:0.1. HBV and HCV infection rate in blood donors were detected at 0.7% (15/2037) and 0.5% (8/2037) respectively. HBV infection rate in volunteer blood donor people was 0.7% (14/1881) which was higher than the replacement donors i.e. 0.6% (1/156). Similarly, in HCV infection rate in volunteer donor were 0.4% (8/1881). HBV infected people are detected higher than the HCV infected people among the blood donors. In addition, there was no significant relationship between positive results of HBV and HCV tests with the gender, age, tattoo, donor type. Keywords: Blood transfusion, Sera, HCV, HBV, ELISA, Nepal *Corresponding Author Email: balkrishna_bhattachan@hotmail.com Introduction Hepatitis B virus (HBV) is an enveloped, hepadnaviridae with partially double-stranded DNA [1]. It is one of the cause of potentially life- threatening liver infection and is a major global health problem. It can cause chronic liver disease and puts people at high risk of death from cirrhosis of the liver and liver cancer [2]. Worldwide, an estimated two billion people have been infected with the Hepatitis B virus and more than 240 million have chronic (long-term) liver infections. In developing countries like Nepal, The highest prevalence of HBV was found in the 6-15 year age group [3]. Common modes of transmission include perinatal, unsafe injection practices, unsafe blood transfusions, and unprotected sexual contact [4]. Hepatitis C virus (HCV) is single-stranded RNA, enveloped flavivirus that causes liver disease [5]. The disease can range in severity from a mild illness lasting for few weeks to a serious, life- threatening condition that can lead from cirrhosis of the liver to liver cancer. About 170 million people are infected worldwide [6]. HCV is most commonly transmitted through exposure to infectious blood and it may be transmitted through gender with an infected person or sharing of personal items contaminated with infectious blood, but these are less common [7]. This study is conducted among blood donors in Pokhara Valley in order to study the HBV and HCV seroprevalence, among different groups and subgroups of blood donors. The main aim of this study is to determine the seroprevalence of HBV and HCV by Rapid kit and ELISA Method and find out its associated risk factors. Open land route facilitates the biological and virological spread of diseases [8]. So, in the case of HCV and HBV, millions of migrant workers act as a biological transporter. However, documentation of HCV and HBV with past infection is frequent Nepal Journal of Biotechnology. D e c . 2 0 1 8 Vol. 6, No. 1: 33-38 Shah et al. ©NJB, Biotechnology Society of Nepal 34 Nepjol.info/index.php/njb in person with routine parenteral exposures which indicate that the transmission by this line is prominent. Accidental parenteral exposures such as needle-stick injuries are quite common in patients. Another route of transmission is having tattoos, drug addicts, heterosexual and homosexual gender, liver diseases, and blood transfusion. Perception and awareness level of people has been changed after the jaundice outbreak occurred in Biratnagar during April-July 2014 [9]. Methods and Materials Study Design and setting Cross-sectional and descriptive type of study was done. The study sample comprised of 2037 blood donors. Healthy donors included in this study were donating blood in Blood Transfusion Center, Pokhara at mobile health camp organized by Red Cross Society. This study was conducted from 16th August to 19th November 2016. Study Area and Population Blood donors were selected according to the criteria of Blood Transfusion Service (BTS) based on the National Guidelines for BTS. The categories of donors that were present in this study were volunteers and replacement who may be the first time or repeated donor. Individuals selected by donor screening criteria as per NRCS’ Standard Operating Procedure (SOP) of 2006 AD. Participants were requested to fill a questionnaire form along with written consent were collected. Each donor was given an ID number for later trace out if needed. Inclusion criteria: Healthy donors were included for blood donation after taking an interview. The detailed inclusion criteria for blood donors are:- donor should be in the age group between 18 to 60 years, body weight more than 45 kg for male and 35 kg for female, within normal range blood pressure 120/80 mm without medication and Hemoglobin should not be less than 12.6 g/ml. Exclusion criteria: Those who are found unhealthy on the interview were excluded for donation. The exclusion criteria for blood donors are:- in the case of a female, menstruating, pregnant and breastfeeding mother are not allowed for donation and Patients taking antibiotics. Sample collection and processing The blood bags were transported from blood donation campaign site to blood bank in igloo box containing ice bars to maintain the temperature near 40C. A few drops of blood is used for blood grouping and 5ml of blood was allowed to clot or centrifuged at 3000 RPM for 2 minutes in a dry screw-capped test tube to separate serum samples to perform the test for HBV and HCV. Detection of HBV and HCV by using Rapid kit test Collected Sera were subjected for routine mandatory screening for HBsAg and anti-HCV antibodies by enzyme immunoassay-based rapid tests according to the standard protocol described by respective company (Hepacard, J. Mitra and Co., New Delhi, India, and HCV TRI- DOT, J. Mitra and Co., New Delhi, India) [10]. Detection of HBV and HCV by using ELISA method Sera from blood donors for HCV were tested against (anti-HCVs) antigen by third generation enzyme-linked immunosorbent assay (ELISA) and for detection of HBV by HBsAg ELISA (HCV TRI-DOT Test, J. Mitra and Co., New Delhi, India; Hepacard, J. Mitra; Genedia HCV ELISA.3.0, Green Cross, Kyunggido, Korea; and Enzygnost HBsAg 5.0, Dade Behring, Marburg, Germany) [11]. Statistical Analysis Pearson's Chi-square test was used to determine the significant association of dependable variables. WinPepi software (version 11.65) was used for quantitative data analysis. A p-value < 0.05 was considered to be statistically significant at 95 % CI. Results A total of 2037 blood samples were collected from donors. The blood was screened by Rapid test and ELISA test to detect HBV and HCV infection. Gender wise, the ratio between male and female was at 1:0.1. The infection rate of HBV and HCV in blood donors were detected to be 0.7 % (15/2037) and 0.5% (8/2037) respectively (Figure 1). Nepal Journal of Biotechnology. D e c . 2 0 1 8 Vol. 6, No. 1: 33-38 Shah et al. ©NJB, Biotechnology Society of Nepal 35 Nepjol.info/index.php/njb Figure 1: Infection of Hepatitis B Virus and Hepatitis C Virus in blood donors (Confirm by ELISA Method) The infection rate in a male for HBV is 0.7% (13/1777) which was higher than HCV 0.4% (8/1777). Similarly, the infection rate in the female for HBV is 0.7% (2/260) which was also higher than HCV (0.0%) but no statistically significant, (p=0.280) result was obtained (Table 1). In HBV, the highest rate of infection was detected 0.9% (7/764) in 31-45 year old of blood donors which was followed by 0.7 % (1/138) in 46-60 year old, and 0.6% (7/1135) in 18-30-year-old people. Similarly, the highest rate of HCV infection were detected 0.7% (1/138) in 46-60 years old people followed by 0.6% (5/764) in 31- 45-year-old and 0.2% (2/1135) in 18-30 years old people, P=0.588, (Figure 2) Figure 2. Age wise distribution with HBV and HCV among blood donors HBV infection rate of volunteer donor people was 0.7% (14/1881), which is higher than the replacement donor 0.6% (1/156). Similarly, HCV infection rate of volunteer donor was 0.4% (8/1881) with p=0.455 (Table 2). Table 2: Donor type-wise distribution with hepatitis infections among blood donors Donor type Disease Total No. p- value HBV No. (%) HCV No. (%) Replacement Donor 1 (0.6) 0 (0.0) 156 0.455 Volunteer Donor 14(0.7) 8 (0.4) 1881 Total 15 (0.7) 8 (0.4) 2037 HBV infection rate in people without tattoo was 0.7% (14/1847) higher than the people with tattoo 0.5% (1/190). Similarly, HCV infection rate in people without tattoo was 0.3% (6/1847) higher than 0.1% (2/190) in people with a tattoo with p=0.214 (Table 3). HBV infection rate of married blood donors was 0.9% (12/1262) whereas 0.3% (3/775) was found in unmarried blood donors. Similarly, HCV infection rate of married blood donors was 0.7% (7/1262) whereas 0.1% (1/775) was found in unmarried blood donor with p=0.65 (Table 4). Table 4. Marital status wise distribution with hepatitis infection among blood donors Disease Total p-value Marital status HBV No. (%) HCV No. (%) No 3 (0.3) 1 (0.1) 775 0.651 Yes 12 (0.9) 7 (0.7) 1262 Total 15 (0.7) 8 (0.4) 2037 Discussion The group of viruses (Hepatitis A, B, C, D, and E) that cause acute and/or chronic infection and inflammation of the liver give rise to a major public health problem globally [12]. Hepatitis B and C viruses are major agents of severe illness and death. The burden of acute hepatitis, liver 0.7 0.4 0 0.2 0.4 0.6 0.8 HBV HCV P e rc e n ta g e Hepatitis types positive (%) Table 1: Sex-wise distribution with Hepatitis diseases among blood donors Gender Hepatitis Total No. p- value HBV No. (%) HCV No. (%) Male 13 (0.7) 8 (0.4) 1777 0.280 Female 2 (0.7) 0 (0.0) 260 Total 15 (0.7) 8 (0.4) 2037 Table 3. Tattoo wise distribution with hepatitis infection among blood donors Disease Total p-value Tattoo used HBV No. (%) HCV No. (%) No 14 (0.7) 6 (0.3) 1847 0.214 Yes 1 (0.5) 2 (0.1) 190 Total 15 (0.7) 8 (0.4) 2037 0.6 0.9 0.7 0.2 0.6 0.7 0 0.2 0.4 0.6 0.8 1 18-30 year 31-45 year 46-60 year P e rc e n ta g e Age group HBV (%) HCV(%) Nepal Journal of Biotechnology. D e c . 2 0 1 8 Vol. 6, No. 1: 33-38 Shah et al. ©NJB, Biotechnology Society of Nepal 36 Nepjol.info/index.php/njb cirrhosis and cancer due to this Hepatitis B and C Viruses is high worldwide (about 2.7% of all deaths) and is forecasted to become a higher ranked cause of death over the next two decades. It is estimated that 57.0% of cases of liver cirrhosis and 78.0% of cases of primary liver cancer have resulted from hepatitis B or C virus infection [13]. The infection rate of HBV and HCV in blood donors were detected on 0.7% and 0.5% respectively. In the previous study reported that the hepatitis B and hepatitis C infection rate was detected by 2.6% and 0.3% respectively out of 307 tested patients [14]. The high percentage of HBV and HCV infection was obtained may be due to less no. of public awareness of hepatitis known infected person did not participated in the blood donation. The unsafe behavioral practices such as body pierce, tattoo, use of injectable drug, unprotected sex etc. also led to increase the transmission of infection. The positive rate of hepatitis infection was found to be higher in a male patient than female but there is no statistical significant relationship found between gender and hepatitis [14]. In HBV infection, most of the infection rate (0.9%) was detected in age group ranging 31-45 years old whereas most of the infection rate in 46-60 years old people in HCV was detected at 0.7%. The highest positive rate was found in the patients of age group 31-60 years old [14]. The infection rate of HBV and HCV in volunteer donor people were found to be 0.7%, and 0.4% respectively. It may be, due to blood transfusion, hepatitis can be transmitted in another body. Study also revealed that the eastern region of Nepal had the highest percentage of HCV infection (48.0%) [15]. Seroprevalence of HBV among mothers were found 17.9% in 151 mothers of the total study population in the selected Village Development Committees (VDCs) [16]. The highest rates of HCV and HBV co-infection was found to be 58% and 70% respectively 30-39 years age group. HBV infection rate was 0.7% higher in people without tattoo whereas the HCV infection rate in people without tattoo was 0.3% higher. Because in our case, the number of participants without tattoo was higher than the number of blood donors with tattoo. Although, it is widely known fact that cosmetic procedure, tattooing on a body can result in a HBV and HCV infection if the needles and equipment used are not properly sanitized. Besides, the poor tattooing procedure may have chances of microbial contamination. HBV and HCV infection rate in married blood donors were 0.9% and 0.7% respectively. Researchers concluded the marital status is a significant risk factor [18]. They detected HBV and HCV were found 1.7% and 1.0% respectively in a married participant. HBV and HCV can be transferred from unprotected sexual contact if their partner is infected with the hepatitis virus. People with unsafe sexual relationships and addiction to injectable drug users should always be careful as the chances of the virus getting transmitted are high among them. This severity of cases may be due to the lack of vaccination information in the population. The replication of HBV and HCV in HIV patients should be actively monitored while receiving antiviral therapy and this monitoring system should be made a part of clinical care [17]. In the context of Nepal, people have has less knowledge of vaccination and those who knows avoid it because of hepatitis vaccination cost and no. of clinic visits to complete vaccination doses. The implications of screening tests for HBV or HCV co-infection in HIV patients are of great need in Nepal [18]. Limitations of the study: This study was carried out in a limited population and only involved the donors attending on Mobile Health camp organized by BTS-NRS. So, the results obtained from this study might not be enough to indicate the actual burden of the disease in the large population. Still, the study found the existence of endemic viral hepatitis among the people and need health policies to reduce the burden of viral hepatitis in society. PCR based detection method was not incorporated due to feasibility issues regarding time and resources constrain. Conclusions HBV and HCV infections can be transfer by piercing needles, general contact, blood Nepal Journal of Biotechnology. D e c . 2 0 1 8 Vol. 6, No. 1: 33-38 Shah et al. ©NJB, Biotechnology Society of Nepal 37 Nepjol.info/index.php/njb transfusion, drug users. Therefore, policymaker should provide awareness and knowledge for people about it. General people must take a hepatitis vaccine. Government should provide hepatitis vaccine at an affordable price. Government and its allied body should provide infection control support. Finally, people should be aware of its associated risk factors. The infection rate in prior blood transfusion people, HBV and HCV infections found in the same ratio. Moreover, due to its risk factors, like age, gender, tattoo, marital status, donor type can be transmitted the vital role in the mode of transmission of hepatitis. Conflict of Interest The authors declare that they have no competing interests. Acknowledgments We would like to convey our gratitude to Mr. Dhrubamadi Lamichhane, coordinator of Central Blood Transfusion Service and all the members of NRCS (CBTS) who were helpful and made my lab work possible. And, thankfulness to Mr. Arjun Gyawali and allied staffs. Without their support, we could not able to conduct this study. Consent for publication Not applicable Ethical approval and consent to the participant No patient-related data were collected. Ethical approval was therefore not required. The study was laboratory-based basic science study. Written informed consent was taken from all participating patients or from guardian on the behalf of their children. Availability of data and materials All supplementary files, data generated and analyzed during this study will be made available as per reasonable request to the corresponding author. Source of Support No funding Author’s Contributions GBS and KG designed the study. GBS collected sample at NRCS-CBTS. GBS and BPB performed investigation and recorded the laboratory findings with the validation. KG supervised and provided a methodology for the study. BPB, BB & RRG administered the project, reviewed literature, and wrote original manuscript BB and RRG curated data to perform statistical data analysis and data interpretation. 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