Microsoft Word - 11502 NSB Ben Rabeh 2023.06.19.docx Received: 00 Xxx 2022. Received in revised form: 00 Xxx 2023. Accepted: 08 Jun 2023. Published online: 19 Jun 2023. From Volume 13, Issue 1, 2021, Notulae Scientia Biologicae journal uses article numbers in place of the traditional method of continuous pagination through the volume. The journal will continue to appear quarterly, as before, with four annual numbers. SHSTSHSTSHSTSHST Horticulture and ForestryHorticulture and ForestryHorticulture and ForestryHorticulture and Forestry Society of TransylvaniaSociety of TransylvaniaSociety of TransylvaniaSociety of Transylvania Ben Rabeh S et al. (2023) Notulae Scientia BiologicaeNotulae Scientia BiologicaeNotulae Scientia BiologicaeNotulae Scientia Biologicae Volume 15, Issue 2, Article number 11502 DOI:10.15835/nsb15211502 ReReReReviewviewviewview ArticleArticleArticleArticle.... NSBNSBNSBNSB Notulae Scientia Notulae Scientia Notulae Scientia Notulae Scientia BiologicaeBiologicaeBiologicaeBiologicae Environmental stress tolerance, hydroEnvironmental stress tolerance, hydroEnvironmental stress tolerance, hydroEnvironmental stress tolerance, hydro----distilldistilldistilldistilled essential oils ed essential oils ed essential oils ed essential oils characteristics and biological activities of characteristics and biological activities of characteristics and biological activities of characteristics and biological activities of Eucalyptus torquataEucalyptus torquataEucalyptus torquataEucalyptus torquata Luehm.Luehm.Luehm.Luehm. Sonia Ben RABEH1,2*, Kaouther Ben YAHIA3, Samir DHAHRI4, Souda BELAÏD1,2, Imen CHEMLALI1,2, Chokri Ben ROMDHANE4, Mehrez ROMDHANE2, Ezzeddine SAADAOUI4 1University of Gabes, Faculty of Science Gabes, Gabes, Tunisia; soniabenrabeh.a@gmail.com (*corresponding author); belaidsouda95@gmail.com; chemlali.imen@gmail.com 2University of Gabes, National Engineering School of Gabes, Laboratory of Energy, Water, Environment and Processes, Gabes, Tunisia; mehrez.romdhane@univgb.tn 3University of Carthage, I National Institute for Rural Engineering, Water and Forestry (INRGREF), LEF, Tunisia; kaoutherbenyahia01@gmail.com 4University of Carthage, National Institute for Rural Engineering, Water and Forestry (INRGREF), LGVRF, Tunisia; dhahri.samir@iresa.agrinet.tn; benromdhanechokri@gmail.com; saad_ezz@yahoo.fr AbstractAbstractAbstractAbstract Eucalyptus has become one of the most widely planted genera in the world because of its tolerance to a wide range of soil types and climates, as well as for its many industrial, commercial and medicinal uses. Eucalyptus torquata Luehm. is a plantation species frequently planted in semi-arid and arid regions for its ecological, forestry, ornamental and melliferous interests. Based on literature, drought tolerance of this species was mostly directed to adaptation mechanisms. Physiological investigations reveal the importance of stomatal closure and increased solute contents suggesting that osmotic adjustment is one of the main responses to drought in E. torquata. On the other hand, it showed low sensitivity to salt stress. This paper also highlights the immense benefits of E. torquata which contains essential oils with variable chemical composition and rich essentially in 1,8-cineole, torquatone, α-pinene, trans-myrtanol, α-eudesmol, β-eudesmol, globulol, trans- pinocarveol and aromadendrene. These oils, as well as the methanol and aqueous extracts possess a wide variety of bioactivities of great importance which are particularly valuable as antibacterial and antifungal agents also have a strong toxicity against insects and mites in addition to antiproliferative and cytotoxic effects against different types of cancer cells. Keywords:Keywords:Keywords:Keywords: biological activities; chemical composition; coral gum; essential oil; Eucalyptus IntroductionIntroductionIntroductionIntroduction Eucalypts (Eucalyptus spp.) are endemic to Australia; however, its few species are indigenous to neighboring countries. The genus Eucalyptus comprises more than 800 species and hybrids, which includes Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 2 shrubs and flowering trees. It is the most valuable genus and it is found in almost all parts of the world due to human introduction (Chemlali et al., 2022). It has been broadly cultivated in many countries to utilize as wood for a diverse range of products and grow across a large range of climatic environments and soil types. Eucalypts are known for their adaptation to arid conditions and are considered drought, salt and heat tolerant compared to other trees (Teulières et al., 2007). The mild climate is the most preferred for most species of Eucalyptus, they are highly distributed where there are warm summers, temperate winters, moderate rainfall, dry atmosphere and plenty of sunlight (Najum Rasheed et al., 2005).The distribution of Eucalyptus all over the world also the dominance in Australia means ecological importance of this genus which provide food and habitat resources for a diverse range of fauna (Vuong et al., 2015). Eucalypts are one of the most bio-economic plant species with high potential to grow in salt-affected soil with arid climatic condition also capable of providing an economic return in the future, it used for ornamental purposes, afforestation, providing feedstock for the pulp and paper industries or to obtain timber and gum, also a nectar resources for honey and known by cosmetic and medicinal values (Saadaoui et al., 2022). In some countries dried Eucalyptus leaves are used as tobacco and smoked for asthma (Sefidkon et al., 2008), aqueous extracts are used for aching joints, bacterial dysentery, ringworms, tuberculosis, etc. (Sefidkon et al., 2010). Also, hot water extracts of dried leaves are traditionally used as analgesic, anti-inflammatory, and antipyretic remedies for the symptoms of respiratory infections, such as cold, flue and sinus (Silva et al., 2003). Eucalyptus has been prized as a rich source of essential oils that’s more useful as it is easily extractable and has advantages as its superior quality and is regarded as safe and non-toxic by the United States Food and Drug Authority (FDA). Eucalyptus oils are volatile organic compounds found in fruits, flowers, bark, seeds, wood and roots, while, these compounds are mainly extracted from foliage (Boland et al., 1991) because in the leaves that oils were most plentiful and more than 300 species of this genus contain volatile oils in their leaves (Pino et al., 2002). Eucalyptus essential oils contain terpenoids, phenolic, flavonoids and alkaloids that possess many bioactivities that could be grouped into three classes viz: perfumery, industrial and medicinal (Abiri et al., 2021). In fact, essential oils rich in 1,8-cineole are utilized as pharmaceuticals, whereas those rich in citronellal, citral and geranyl acetate are used in perfumery (Dhakad et al., 2018). Under natural conditions, essential oils from the leaves of Eucalyptus known to provide allelopathic property to this plant (May and Ash, 1990) and defense to Eucalyptus leaves against attack by harmful insects, and thus acts as a natural pesticide (Batish et al., 2008; Üstüner et al., 2018; Gallon et al., 2020; Sadraoui-Ajmi et al., 2022). In fact, many researchers reviewed the biological properties of Eucalyptus essential oils including anti-microbial, fungicidal, antiviral, insecticidal/insect repellent, herbicidal, acaricidal and nematicidal also the anti-tumour and cytotoxic activities (Zhang et al., 2010; Vuong et al., 2015; Barbosa et al., 2016; Dhakad et al., 2018; Salehi et al., 2019; Abiri et al., 2021; Chandorkar et al., 2021). The importance and the commercial uses of essential oils of Eucalyptus have increased the research on their extraction, exploring their chemical compositions and bioactivities. In fact, Eucalyptus torquata Luehm. commonly known as coral gum or coolgardie gum is an attractive tree with a small to medium size growing to 6-8 m and a spread of some 5 m, presenting a single trunk, a greyish green foliage and the blade has a lanceolate shape. The length of leaves around 90-120 mm and wide of 15-20 mm, the profuse flowers are reddish-pink or coral colored and hang decoratively on reddish stems. Flowering is very conspicuous and occurs in spring to summer (Al-Snafi, 2017). It is a fast-growing tree known for tolerance to drought, often hybridising in cultivation with another commonly grown arid zone species, E. woodwardii to give a hybrid E. torwood. It recommends planting E. torquata in protected areas as an ornamental tree in the public and private gardens due to its beautiful flowers and its medium size (El-Juhany and Al Al- Shaikh, 2015). The researchers were also exploring the potency of E. torquata by valuing their extracts and essential oils which show different biological activities such as antibacterial, antifungal (Ashour, 2008), cytotoxic (Ashour, 2008; Bardaweel et al., 2014; Lahmadi et al., 2021) and pesticidal activity (Ebadollahi et al., 2017; El Finti et al., 2022; Ebadollahi et al., 2022). Also, E. torquata considered an important sources of nectar Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 3 and pollen for honeybees as showing an abundant flowering of long period and good quality of pollen and nectar for the nutrition of bee; for these reasons, it is frequently planted in arid regions (Eisikowitch et al., 2012; Saadaoui et al.,2022 ). The purpose of this study is to provide the readers with information concerning the tolerance and behavior of E. torquata under drought and salt stress, also, exploring the potency and diversity of extracts and essential oils of this species in terms of chemical composition and biological activities. Evaluation of the tolerance Evaluation of the tolerance Evaluation of the tolerance Evaluation of the tolerance of of of of E. torquataE. torquataE. torquataE. torquata to drought and salt stressto drought and salt stressto drought and salt stressto drought and salt stress Drought and the salinization of soil are a widespread environmental problems and an important factors determining plant productivity and distribution (Teulières, 2010).For landscape applications like reclamation of dry and arid saline lands, Eucalyptus is a good choice, it’s a versatile woody species that develops an extensive deep root system and presents the challenge of finding a good compromise between adaptation to specific environmental conditions and productivity (Teulières et al., 2007). Responses to drought Drought is the second productivity-limiting stress after cold to find subsequently biotic and abiotic stresses, it was suggested that the availability of water is the important determining factor for the distribution of Eucalyptus (Li and Wang, 2003). In fact, among 117 Eucalyptus species introduced in Tunisia, E. torquata is considered a drought-resistant species (Khouja et al., 2001; Saadaoui et al., 2017). Australian Native Plants Nursery (2015) mentioned that E. torquata is tolerant of extended dry periods (El-juhany et al., 2008), also, in Saudi Arabia it was classified among the high tolerating species to drought (El-juhany and Al Al-Shaikh, 2015). In the Mediterranean arid regions, E. torquata showed a high tolerance level and flower abundance also in the southern provinces of North Africa (Chemlali et al., 2022). Mechanisms employed for drought resilience of E. torquata were investigated by Souden et al. (2020) which reported physiological and biochemical responses of this species subjecting to a dehydration period followed by rehydration. It reported that E. torquata was less resilient to drought than E. camaldulensis. Nevertheless, common responses were shown during the dehydration phase including lowering cell water potential from -1MPa to -4.9MPa after 28 days and to - 7.1MPa after 45 days of no irrigation which was restored with 88% after rewatering. In the face of water stress, lowering the water potential of the cells by the plant, help to maintain the water content of the cells and, consequently, the turgor (White et al., 2000). Other physiological responses for E. torquata are observed including the early closure of stomata which starting from -3.5MPa to prevent water loss, the net photosynthesis was decreased to achieve less than 2 μmol.m⁻2.s⁻1. The chlorophyll fluorescence parameters Fv/Fm (maximum photochemical efficiency of PSII) was decreased and after 30 days of re-watering, E. torquata restored the structural and functional integrity of its photosynthetic machinery. Changes in xylem conductivity under water deficit also showed which conducted to minimal xylem embolism for E. torquata and the value of Ψ xylem which induced 50% PLC (Ψ50) is-4.6MPa, obviously, the level of xylem cavitation decreased after rehydration (Souden et al., 2020). It has been shown also that in case of drought and/or salinity, osmotic adjustment is the key to the adaptation of plants at the cellular level this by the accumulation of organic and inorganic solutes which helps to reduce the water potential without reducing the actual water content (Sanders and Arndt, 2012). For E. torquata, water stress induced accumulation of soluble sugars (glucose and fructose) and cyclitols (pinitol, myo-inositol) for its osmotic adjustment (Souden et al., 2020). These adaptive traits are the key factor in the determination of E. torquate drought resistance. Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 4 Responses to salinity Another major stress for plants is the salinity of soils. In fact, the exposure to salt stress triggers many common reactions in Eucalyptus species which have developed several strategies to cope with these challenges (Assareh, 2016). However, three strategies for achieving greater salt tolerance: damage prevention, homeostasis establishment and growth regulation (Zhu, 2001). How E. torquata deal with and respond to salinity stress has been reported by Balti et al. (2021) and the study showed that E. torquata was the salt-sensitive even at lower salt concentrations (80 mM NaCl) among other species such as E. gomphocephala and E. loxophleba. Salt stress induces certain biochemical and physiological changes in E. torquata, also visible symptoms mainly by the development of necrotic spots in leaves after exposure to 170 mM of NaCl for 30 days which indicates salt- induced damage at cellular level. Slower growth was not observed for E. torquata indicating the inability of growth modulating under salt stress. Changes in photosynthesis also observed, salt stress majorly affect optimal protein function in the photosynthetic electron transport chain (pETC). The chlorophyll fluorescence-based PSII-related parameters calculated showed lower values, in addition to that, a decline in chlorophyll and carotenoids contents in leaves has been observed. The K+/Na+ ratio for E. torquata declined significantly than other species mainly for E. loxophleba which have the ability to selectively increase K+ amounts over Na+ (Balti et al., 2021). Moreover, NaCl salinity causes a significant effect on Na+, K+ and Cl- uptake and their distribution, in this, higher levels of external Na+ interfere with K+ acquisition limiting plant K uptake. Therefore, one of the important physiological mechanisms for salinity tolerance is the K+ selective absorbance (Nasim et al., 2008). Germination also is strongly influenced by osmotic pressure caused by salts in the soil solution (Madsen and Mulligan, 2006). Mechergui et al. (2019) reported that seeds of E. torquata were not able to germinate at up to 9, 12 and 15 g.L⁻1 NaCl that means that the salinity levels influenced significantly the percentage of germination. For the responses of E. torquata to salt stress in relation to growth, it reported that this species showed low survival percentages and volume growth to age 20 years under salt water irrigation (El-Juhany and Al Al-Shaikh, 2015). All these results suggest a good drought tolerance of this forest species; however, it shows a relative sensitivity to the presence of NaCl in the growing medium and soils. Yields of Yields of Yields of Yields of E. torquataE. torquataE. torquataE. torquata on essential oils on essential oils on essential oils on essential oils The essential oils of E. torquata may be obtained from different plant parts; however, as observed in Table1, the highest was found in the leaves whose production was much higher than that in the trunk bark. Hydro distillated leaves of E. torquata ranged 1.15-3% of essential oil. Similar essential oils yield (1.21-3.1%) has been reported for E. globulus, as the principal source of Eucalyptus oil in the world (Derwich et al., 2009; Mossi et al., 2011; Mulyaningsih et al., 2011; Harkat-Madouri et al., 2015). The geographical origin also highly affects this production; in this, good extraction yields were observed for plants from Tunisia. In fact, several studies reviewed the parameters that can influence the total essential oil content of plant including part of plant (Silva et al., 2011), geographic origin (Gilles et al., 2010; Almas et al., 2018), the seasonal variations (Silva et al., 2011), the phenological stage (Salem et al., 2018), method of extraction (Ben Hassine et al., 2010; Herzi et al., 2013; Chamali et al., 2021), rainfall and harvesting regime (Gilles et al., 2010). Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 5 Table 1. Table 1. Table 1. Table 1. Essential oil yields in E. torquata obtained by hydro distillation in Tunisia, Iran, Morocco and Cyprus OriginOriginOriginOrigin Part usedPart usedPart usedPart used Harvest period/sample crushed Harvest period/sample crushed Harvest period/sample crushed Harvest period/sample crushed or notor notor notor not Essential oils Essential oils Essential oils Essential oils yields (%)yields (%)yields (%)yields (%) ReferencesReferencesReferencesReferences Tunisia Trunk bark February 2019/Dry sample ground into a fine powder 0.006 (Lahmadi et al., 2021) Leaves January 2005/Dry leaves boorishly crushed 3.2 (Elaissi et al., 2010) January 2007/Dry leaves medially crushed 1.86 (Ben Hassineet al., 2010) Iran Dry leaf powder 1.15 (Ebadollahi et al., 2017) Morocco April 1991 1.17 (Zrira et al., 1994) Cyprus March 2021/ Dry leaves crushed into tiny pieces 1.6 (Yiğit Hanoğlu et al.,2022) Chemical profiling of Chemical profiling of Chemical profiling of Chemical profiling of E. torquataE. torquataE. torquataE. torquata essential oilsessential oilsessential oilsessential oils Essential oils obtained from Eucalyptus are usually rich in monoterpenes and in some cases sesquiterpenes. Nevertheless, the chemical profile and main components of oils from Eucalyptus varied significantly between species. Mostly, the main components were the oxygenated monoterpenes 1,8-cineole and the monoterpene hydrocarbons α-pinene with various percentages dependent on the specific species (Goldbeck et al., 2014; Ishnava et al., 2013). Other compounds also are detected as major component in Eucalyptus oils as example; limonene in E. crebra oils, citronellal in E. citriodora oils (Ghaffar et al., 2015) and p-cymene in E. oleosa (Chamali et al., 2019). In Tunisia, most of Eucalyptus species oils showed that the oxygenated monoterpenes constituted the major fraction as the 1,8-cineole was the major component (Elaissi et al., 2010; Elaissi et al., 2011a, 2011b, Elaissi et al., 2011; Elaissi et al., 2012, Elaissi et al., 2012; Sebei et al., 2015; Limam et al., 2020; Ameur et al., 2021). A wide number of terpenes have been identified in the leaves essential oil of E. torquata, using analyses by GC-FID, GC or GC-MS (Table 2). In spite of current variations of the origin of the analyzed plants, there is consistence that 1,8-cineole and α-pinene are a characteristic compound of this species. 1,8-cineole was isolated in concentrations between 11 and 70% also the α-pinene obtained with concentration between 10 and 20%. Other compounds detected such as trans-pinocarveol, α-terpineol and borneol from chemical class of oxygenated monoterepenes. The oils also contain considerable amount of the monoterpene hydrocarbons p- cymene, also the aromadendrene and alloaromadendrenefrom chemical class of sesquiterepene hydrocarbons. α-eudesmol, β-eudesmol, γ-eudesmol and globulol are the main oxygenated sesquiterpenes. A high percentage of torquatone also was detected. This last compound forms a member of acylphloroglucinols which was a class of specialized metabolites with relatively high content in Eucalyptus with diverse structures and bioactivities (Singh et al., 2009; Yao et al., 2021). Torquatone was first isolated from E. torquata and E. caesia Benth growing in Australia with 25 and 50% of the essential oil fraction respectively (Bowyer and Jefferies, 1959). It derivative also from the essential oils of number of Eucalyptus species and absent in others and present with relative high concentration in E. torquata (Ghisalberti, 1996; Bignell et al., 1997a, 1997b; Elaissi et al., 2010; Yiğit Hanoğlu et al., 2022). The chemical formula of torquatone is C16H24O4; there is 4,6-trimethoxy-3,5-dimethyl-1-(3- methylbutyroyl)-benzene (Menut et al., 1999; Figure 1). When the composition of twelve Eucalyptus essential oils (E. torquata Luehm, E. woodwardi Maiden, E. stricklandii Maiden, E. occidentalis Endl, E. brockwayi C. A. Gardn, E. salmonophloia F. Muell, E. gillii Maiden, E. oldfieldii F. Muell, E. largiflorens F. Muell, E. loxophleba Benth, E. sargentii Maiden, E. gracilis F. Muell,) are compared, the high percentage of torquatone is recorded Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 6 for E. torquata with 42% also the low percentage of sesquiterpenes hydrocarbons and oxygenated monoterpene with a low quantity of 1,8-cineole (12%) and a relative high amounts of α-pinene (10.5%), α-eudesmol (2.9%), β-eudesmol (10.1%) and γ-eudesmol (1.3%). Eucalyptus woodwardii oil had resembling chemical characteristics to E. torquata oil essentially in major compounds detected (Elaissi et al., 2010; Ben Amor, 2021). Torquatone 1,8-Cineole α-Pinene trans-Myrtanol Figure 1. Figure 1. Figure 1. Figure 1. Chemical structure of major elements of essential oil of E. torquata Plants cultivated in different countries produce essential oils with variable composition as can be seen from Table 2. Torquatone is detected as a major component of E. torquata leaves essential oils from Tunisia (42%), Australia (42%) and Cyprus (29%) while totally absent in oils from Iran and Morocco. The same for α- eudesmol, β-eudesmol and γ-eudesmol that are not detected in Iranian species. An intra-specific variation is also recorded and explained by geographical, environmental and climatic variations which affect the chemical composition of essential oils. Also, it was proven that essential oils of different plant parts have different chemical composition (Table 2). The trunk bark essential oil of E. torquata growing in Tunisia has a completely distinct chemical profile compared to the leaf essential oils. The 1,8-cineole was totally absent and the major constituents being the oxygenated monoterpenes (84.7%), with trans-myrtanol (73.4%) and myrtenol (4.7%) as the main components. The apocarotene cis-β-ionone and the fatty acid nonanoic acid also identified in significant percentages of 3.9% and 2.4% respectively, the sesquiterpene hydrocarbons were represented with only 2% with γ-maaliene as the main component (1.3%) (Lahmadi et al., 2021). Therefore, there are notable quantitative and qualitative differences in E. torquata essential oils compositions; it is mentioned in literature that these differences are attributed to several exogenous factors: harvest time, seasonal factors, soil composition, geographical position and the method of drying of plants. Endogenous factors are involved including genetic makeup and the ontogenetic development stage (Marzoug et al., 2011; Zandi-Sohani and Ramezani, 2015). Since the chemical composition of the Eucalyptus essential oils is directly associated with their biological activities, the following discussion will be focused on such activities of E. torquata. The specific and different composition in E. torquata can only act on these activities. Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 7 Table 2. Table 2. Table 2. Table 2. Major components of E. Torquata essential oils obtained by hydro distillation from Tunisia, Iran, Australia, Morocco and Cyprus OriginOriginOriginOrigin Part usedPart usedPart usedPart used Major Components (%)Major Components (%)Major Components (%)Major Components (%) Identification Identification Identification Identification methodmethodmethodmethod ReferencesReferencesReferencesReferences Tunisia Trunk bark trans-Myrtanol (73.4), myrtenol (4.7), (E)-β-ionone (3.9), nonanoic acid (2.4), α-terpineol (1.9), decanoic acid (0.9), γ- maaliene (1.3), cis-myrtanol (1.2), β- cyclocitral (0.8), geranylacetone (0.8), (E)-ocimenol (0.7) (GC-EI-MS) (Lahmadi et al., 2021) Leaves Torquatone (42), 1,8-cineole (12), α- pinene (10.5), β-eudesmol (10.1) trans- pinocarveol (5.1), α-eudesmol (2.9), p- cymene (2), globulol (2), γ -eudesmol (1.3), aromadendrene (1.1), δ-cadinol (0.9) (GC (RI) and GC/MS) (Elaissi etal., 2010) Iran 1,8-Cineole (69.6), α-pinene (9.5), terpinen-4-ol (0.8), α-terpineol (1.1), alloaromadendrene (7.8), aromadendrene (4.5), limonene (1.5), p-cymene (0.7) GC-FID and GC- Mass (Nikbakht et al.,2015) 1,8-Cineole (66.9), α-pinene (13.9), trans-pincarveol (6.3), p-cymene (4.2) (GC and GC/MS) (Sefidkon et al., 2010) 1,8-Cineole (28.57), α-pinene (15.74), globulol (13.11), alloaromadendrene (7.26) α-terpineol (2.64), , , , epiglobulol (2.50), p-cymene (2.46), trans- pinocarveol (2.09), viridiflorol (1.86), endo-borneol (1.72), neoalloocimene (1.53), terpineol-4 (1.51), ledene (1.39), α-gurjunene (1.25), , , , δ-selinene (1.04) (GC-MS) (Ebadollahi et al., 2017) 1,8-Cineole (24.2), α-pinene (20) globulol ((((8.4), aromadendrene (7.8), α- terpineol (2.5), cubeban-11-ol (2.4), trans-sabinol (2), alloaromadendrene (1.8) (GC-MS) (Ebadollahi et al., 2022) Australia Torquatone (42), 1,8-cineole (11.2), α- pinene (10.2), α-eudesmol (10.2), β- eudesmol (11.1), γ-eudesmol (4.8) (GC-FID and GC- MS) (Baranska et al., 2005) Morocco 1,8-cineole (46,9), α-pinene (16,7), bornéol (10.8), 4-terpinéol (3,2), globulol (1.6), p-cymene (1.3) (GC-FID) (Zrira et al., 1994) Cyprus Torquatone (29.2), 1,8-cineole (18.8), α- pinène (18.6), β-eudesmol (10.3), α- eudesmol (6.8) (GC and GC-MS) (Yiğit Hanoğlu et al., 2022 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 8 Biological activitiesBiological activitiesBiological activitiesBiological activities Antimicrobial activity of E. torquata essential oils Eucalyptus essential oils endowed antimicrobial action against a large spectrum of bacteria and fungi which consist to its therapeutic properties as a promising alternative to drugs for several diseases and disorders (Zhang et al., 2010; Barbosa el al., 2016; Dhakad et al., 2018). Additionally, the possible interactions of Eucalyptus essential oils with conventional antimicrobial agents was studied that could lead to new treatment strategies involving reduced antibiotic doses and for higher therapeutic efficacy (Knezevic et al., 2016; Scazzocchio et al., 2016; Al-Qaysi el al., 2020). The bioactivity of Eucalyptus essential oils may be due to their monoterpene components; in fact, antimicrobial activity could be attributed to the presence of compounds such as 1,8-cineole, α-pinene, β-pinene and limonene (Dhakad et al., 2018). However, the interactions of different constituents may be responsible for the total bioactivity of Eucalyptus essential oils that can potentially lead to additive, synergistic, or antagonistic effects (Mulyaningsih et al., 2010). Eucalyptus torquata essential oils marked antimicrobial activities against a large spectrum of bacteria based on agar diffusion method and the microdilution method (Table 3) the bioassays confirm that Gram- positive bacteria are more sensitive compared to Gram-negative ones. Indeed, leaves, stems and flowers essential oils of E. torquata exhibited a moderate to high antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Bacillus subtilis with inhibition zones in the range of 10 and 22 mm of diameter and against the two bacteria Klebsiella pneumoniae and Proteus mirabilis with inhibition zones in the range of 8 and 10 mm of diameter while not active against salmonella typhi. Also, E. torquata flowers essential oil demonstrated antibacterial action against Pseudomonas aeruginosa with inhibition zone of 11mm (Ashour, 2008; Bardaweel et al., 2014) this last bacteria was resistant to essential oils obtained from several Eucalyptus species and from other plants (Elaissi et al., 2011; Wilkinson and Cavanagh, 2005). In other hand, Pseudomonas aeruginosa with Escherichia coli are resistant to leaves essential oils of E. torquata grown in Egypt while susceptible to that grown in Jordan with inhibition zones of 11 and 9 mm respectively (Ashour, 2008; Bardaweel et al., 2014). Minimum inhibitory concentration (MIC) of leaves essential oils of E. torquata from Jordan was calculated by the microdilution method, Norfloxacin 1 mg/ml was used as reference controls for antibacterial activity. The MIC values for Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeuriginosa are 198, 201, 197, 204 and 217 μg/ml respectively (Bardaweel et al., 2014). E. torquata essential oils also cause growth inhibition of some fungal species, oils from flowers, stems and leaves of E. torquata from Egypt and Jordan exhibited a moderate to high antifungal activities against mycelial fungi Aspergillus flavus and Aspergillus nigeralso against the yeast Candida albicans. Flowers essential oil from Egypt showed the maximum zone inhibition against Aspergillus flavus and Candida albicans with inhibition zones of 17 and 15 mm respectively, while the leaves essential oil is active with inhibition zones of 10 and 14 mm respectively. Nevertheless, essential oils from Jordan are active against Aspergillus flavus and Candida albicans with inhibition zones of 10 mm and with MIC values of 198 and 192 μg/ml respectively (Ashour, 2008; Bardaweel et al., 2014). As a result, the difference in the chemical composition of E. torquata essential oils shown previously could be the cause of the difference in their biological and therapeutic activities. Antimicrobial activities of E. torquata extracts Hence, there is an urgent need to find alternative antimicrobial agents for the treatment of resistant pathogenic microorganisms. The use of plant-based antimicrobials has several advantages over synthetic chemicals since the lower incidence of numerous side effects, low toxicity for mammals and high degradability Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 9 (Raja, 2014). Eucalyptus species are known to be a rich source of bioactive compounds, including phenolic, flavonoid, terpenoids, tannins, phloroglucinol and cardiac glycosides, which had potential antimicrobial activities (Luís etal., 2016; Elansary et al., 2017; Bhuyan et al., 2017; Sabo and Knezevic, 2019). Indeed, phenolic compounds are those which contribute significantly to the antioxidant activities of plant extracts (Siramon and Ohtani, 2007; Ghaffar et al., 2015). Two studies conducted in Morocco revealed that aqueous extracts of powdered waste from E. torquata ( leaves, stems, twigs and other parts) contained total polyphenols amounts of 73.48 and 76.68 mg GAE/g DW and flavonoids content of approximately 58 mg RE/g DW in which significant antioxidant capacity has been investigated for these extracts (Bouhlali et al., 2020; Bouhlali et al., 2021). Additionally, methanol and aqueous extracts from leaves, stems and flowers of E. torquata showed antibacterial action against different medically bacteria such as Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Bacillus subtilis and Escherichia coli with different inhibition zones in the range of 7 and 25 mm, also, it marked an antifungal activity against the yeast Candida albicans with inhibition zones in the range of 9 and 14 mm of diameter (Ashour, 2008). In another way, the growing interest in the use of natural plant products in the biological control of plant disease through the use of biological methods has been a great challenge for agriculture for a long time. In fact, Eucalyptus species possess fungicidal properties against large spectrum of phytopathogenic fungi (Zhou et al., 2016; Gakuubi et al., 2017; Abdelkhalek et al., 2020). Also, aqueous extracts from E. torquata waste (leaves, branches, twigs) showed an antifungal activity against two fungal pathogens Fusarium oxysporum f. sp. albedinis and Mauginiella scaettae in a dose dependent manner and more stronger than extracts from other plants such as Acacia cyanophylla, Cupressus atlantica, Nerium oleander and Schinus molle (Bouhlali et al., 2020; Bouhlali et al., 2021). These soil-borne fungal pathogens caused a serious threat to date palm (Phoenix dactylifera L.) in Morocco. “Bayoud” disease and inflorescence rot are the principal enemy of palm trees caused by these pathogens and researchers suggest the use of this plant to control these diseases. It reported that at every concentration tested, E. torquata extract showed the strongest inhibition activity on fungal mycelia growth of pathogens. At a dose of 4% of extract, the spore germination of Fusarium oxysporum inhibited with 79.21% after 7 days of incubation and strong sporulation reductions is shown with 44.97% of extract after 10 days of incubation (Bouhlali et al., 2020). 100% inhibition of spore germination of Mauginiella scaettae at a low concentration of 1% of E. torquata extracts after 24h of incubation and a great reduction in sporulation by 88.05% at a dose of 4%. The inhibitory effect of these extracts is related to their composition; moreover, content in polyphenols and flavonoids in aqueous extracts of E. torquata are found to be correlated with this antifungal activity as well as their antioxidant properties (Bouhlali et al., 2021). Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 10 Table 3. Table 3. Table 3. Table 3. Biological properties of E. torquata essential oils and extracts E. torquataE. torquataE. torquataE. torquata effectseffectseffectseffects Plant part Plant part Plant part Plant part (extract or oil)(extract or oil)(extract or oil)(extract or oil) Tested organism Tested organism Tested organism Tested organism and /or cell lineand /or cell lineand /or cell lineand /or cell line Effects and/or Effects and/or Effects and/or Effects and/or related mechanisms related mechanisms related mechanisms related mechanisms aaaa ReferencesReferencesReferencesReferences Antimicrobial Antimicrobial Antimicrobial Antimicrobial activityactivityactivityactivity Leaves essential oil from Jordan B. subtilis, S. aureus S. epidermidis P. aeuriginosa E. coli C. albicans, A. flavus Growth inhibition IZD MIC 11 198 12 201 10 197 9 204 11 217 10 192,198 (Bardaweel et al., 2014) Leaves, stems and flowers essential oils from Egypt B. subtilis S. aureus S. epidermidis E. faecalis K. pnemounia P. mirabilis C. albicans A. flavus A. niger 11-15 10-16 19-22 12-19 8-10 8-9 10-15 14-17 12-15 (Ashour, 2008) Flowers essential oil from Egypt P. aeuriginosa 11 Leaves, stems and flowers methanolic extract from Egypt S. aureus S. epidermidis E. faecalis B. subtilis, E. coli C. albicans 16-17 10-16 13-19 13-15 10-11 Leaves, stems and flowers aqueous extract from Egypt S. aureus S. epidermidis E. faecalis B. subtilis C. albicans 10-13 9-13 18-24 11-14 9-10 AnticancerAnticancerAnticancerAnticancer activityactivityactivityactivity Stems and leaves essential oils from Egypt MCF7 Human breast adenocarcinoma cells Notable cytotoxic effect with IC50 values of 1.34(stem oil) and 5.22 μg/mL (leaf oil) Leaves essential oil from Jordan Nine mammalian cell line (MCF-7, HeLa, Caco, T47D, BJAB, Raji, A498, PC3 and Caki) Cytotoxic effect with IC50 values ranging from 33 and 115 μg/mL after exposure time of 48 h Probably involved cell death by apoptosis (Bardaweel et al., 2014) Trunk bark essential oil from Tunisia Two human cell lines MDA-MB-231 and SW620 Inhibitory effect on cell proliferation with IC50 values of (Lahmadi et al., 202) Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 11 40.66 and 26.71 μg/mL after 48h Insecticidal activityInsecticidal activityInsecticidal activityInsecticidal activity Aqueous extract of leaves from Morocco D. opuntiae 65% mortality in the population of adults females and 50% of nymphal stage after three application of extract (60%) (El Finti et al., 2022) Leaves essential oil from Iran R. dominica Fumigant toxicity after 72h with LC50 and LC90 values of 31.567 and 105.017 μL/L of Air respectively Ebadollahi et al., 2022) Acaricidal activityAcaricidal activityAcaricidal activityAcaricidal activity Leaves essential oil from Iran T. urticae Strong fumigant toxicity against female adults (LC50 = 3.59 (μl/l air after 24h) (Ebadollahi et al. (2017) aIZDIZDIZDIZD – Inhibitory zone diameter (mm), MICMICMICMIC- minimal inhibitory concentration (μg/ml), IC50IC50IC50IC50- 50% inhibitory concentration, LC50LC50LC50LC50-50% lethal concentration, LC90LC90LC90LC90 - 90% lethal concentration. Anticancer activity The cytotoxic effect of extracts and components isolated from different species of Eucalyptus has been studied by several researchers. Anti-tumor properties of phenolics, terpenoids (monoterpenes, sesquiterpenes, diterpenoids and triterpenoids) derived from Eucalyptus plants have been discussed by Abiri et al. (2021) which explain the broad spectrum of toxicity, antitumor properties, and mechanisms against cancerous cell lines of Eucalyptus-derived essential oil which can be a promising green anti-cancer drugs. Also, Bardaweel et al. (2014) demonstrated the cytotoxic effect of essential oils from E. torquata gorwn in Jordan which reported that it was varied and highly cell line dependent; in fact, various cytotoxicity levels have been observed on the cancer cell lines treated with essential oil after exposure time of 48 h at 37 °C based on MTT assay. It reported that the EBV-negative Burkitt’s lymphoma BJAB cell and the human Burkitt’s lymphoma Raji cell line are the most sensitive cell lines with IC50 values of 33 and 39 μg/ml respectively. Although, cytotoxic proprieties also observed against the human breast adenocarcinoma MCF7 cell line (IC50 values of 115 μg/mL), the human ductal breast epithelial tumor cell line T47D (IC50 values of 82 μg/mL), the human clear cell renal cell carcinoma Caki cell (IC50 values of 94 μg/mL), the human kidney carcinoma cell line A498 line (IC50 values of 87 μg/mL), the human prostate cancer PC3 cell line (IC50 values of 108 μg/mL), the human colon adenocarcinoma Caco-2 cell line (IC50 values of 108 μg/mL) and the human epithelial carcinoma HeLa cell line (IC50 values of 91 μg/mL). Also, the Lactate dehydrogenase (LDH) activity and the decrease in DNA content of cell line treated indicated that the cytotoxic activity of E. torquata essential oils probably mediated through induction of cell death by apoptosis (Bardaweel et al., 2014). The trunk bark essential oil of E. torquata grown in Tunisia displayed significant antiproliferative effect against two human cancer cell lines: breast carcinoma cell lines MDA-MB-231 and colorectal cancer cell lines SW620 which demonstrated inhibitory effect on the tested cell lines proliferation in a dose-dependent manner after 48 h of incubation using Crystal Violet Staining (CVS) assay, the highest cytotoxic activity of essential oil is observed at 100μg/mL and it's shown that colon carcinoma cells are more sensitive against essential oils (with IC50 values of 26.71 μg/mL) than breast MDA-MB-231 (with IC50 values of 40.66 μg/mL) (Lahmadi et al., 2021). According to the protein-staining sulphorhodamine B (SRB) assay for cell growth, essential oils of E. torquata from Egypt (extracted from stem with IC50 value of 1.34 μg/mL and leaves with IC50 value of 5.22 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 12 μg/mL) have a cytotoxic effect on the Human breast adenocarcinoma cell line (MCF7) and failed to exert a considerable effect on Human hepatocellular carcinoma cell line (HEPG2) (Ashour, 2008). These studies increase the attention in exploring this species and improving the therapeutic opportunities against cancer. E. torquata as pesticide Attacks and infection by pests (especially weeds, pathogens and animal pests) are the largest competitor of agricultural crops that severely reduce crop productivity (Oerke, 2006). However, the excessive use of synthetic pesticide residue in food, accumulated in the environment and increasing health hazards to humans in addition to the increasing risk of pesticide resistance (Pimentel et al., 1992), is thus pertinent to explore the pesticidal activities of natural products. Eucalyptus species are known to be a rich source of bioactive compounds that allow it to act directly as natural pesticide (Radwan et al., 2000; Shukla et al., 2002; Batish et al. 2008; Anita et al., 2012; Barbosa et al., 2016; Adak et al., 2020). Absolutely, E. torquata has been shown insecticidal properties against the Cochineal, Dactylopius opuntiae, an insect that highly damaging the cactus plants in Morocco, , , , it found that three applications of aqueous extract (60%) of E. torquata leaves are needed to reduce mealybug populations also caused the death of 65% of females and 50% of nymphal stages of Dactylopius spp. after 72 h after spraying with E. torquata extract which could be an alternative for the control of wild cochineal (El Finti et al., 2022). Also, E. torquata essential oils showed a great insecticidal potential on the adults of Rhyzopertha dominica, an insect pest of stored products, in which significant fumigant toxicity against insect which was augmented by increasing the concentration of E. torquata essential oils and the exposure time. The LC50 value decreased with increasing exposure time from 37.728 (µL/L of Air) after 24 h to 31.567 (µL/L of Air) after 72 h of exposure with essential oil. In fact, sublethal biochemical disruption has been shown in treated insects, including the reduction of energy content resulting from the significant decrease of the protein and glycogen contents. In other hand, an inhibition of digestive amylase and protease enzyme activities, also, a significant decreases in the relative growth rate of insects (Ebadollahi et al., 2022) which confirm that E. torquata extract and essential oils can be used to control insect pests. Also, it found that possess acaricidal properties as reported by Ebadollahi et al. (2017) which demonstrated that E. torquata leaves essential oils have strong toxicity against the adult females of Tetranychus urticae Koch and the observed LC50 values in the fumigation test was 3.59 μL/L air after 24 h. ConclusionsConclusionsConclusionsConclusions With many Eucalyptus species adapted to arid conditions, E. torquata is considered drought tolerant. The corresponding tolerance mechanisms developed by this species were demonstrated in this review. To assume, drought resistance of E. torquata was manifested by stomatal closure to prevent water loss. Osmotic adjustment was a coping strategy to water stress by increasing the accumulation of solutes, including soluble sugars (glucose and fructose) and cyclitols (pinitol and myo-inositol) in addition to that, the resilience to xylem embolism. However, salt stress may have a negative impact on E. torquata which appears sensitive to the presence of NaCl that acts in particular on photosynthesis. This paper showed also the large variability in yields and chemical composition that exists among E. torquata hydro distillated essential oils from several origins. The majority of oils produced are rich in 1,8-cineole, α-pinene and torquatone. It can be concluded that E. torquata derived essential oils are a rich resource of active phytochemicals which can possess a wide range of biological activities; From the present review, it is clear that possess potent antimicrobial capacity and exhibit an advances anticancer and biocidal effects. Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 13 Authors’ ContributionsAuthors’ ContributionsAuthors’ ContributionsAuthors’ Contributions Writing-original draft: SBR; drafting guidance: KBY, SD, IC, SB, CBR; Supervision: ES, MR. All authors read and approved the final manuscript. Ethical approvalEthical approvalEthical approvalEthical approval (for researches involving animals or humans) Not applicable. AcknowledgementsAcknowledgementsAcknowledgementsAcknowledgements This work was by the INRGREF (Agricultural Experimentation Unit of Gabes). Conflict of InterestsConflict of InterestsConflict of InterestsConflict of Interests The authors declare that there are no conflicts of interest related to this article. ReferencesReferencesReferencesReferences Abdelkhalek A, Salem MZM, Kordy AM, Salem AZM, Behiry SI (2020). Antiviral, antifungal, and insecticidal activities of Eucalyptus bark extract: HPLC analysis of polyphenolic compounds. Microbial Pathogenesis 147:104383. https://doi.org/10.1016/j.micpath.2020.104383 Abiri R, Atabaki N, Sanusi R, Malik S, Abiri R, Safa P, … Abdul-Hamid H (2021). New insights into the biological properties of Eucalyptus-Derived essential oil: A promising green anti-cancer drug. Food Reviews International 1- 36. https://doi.org/10.1080/87559129.2021.1877300 Adak T, Barik N, Patil NB, Govindharaj GPP, Gadratagi BG, Annamalai M, … Rath PC (2020). Nanoemulsion of Eucalyptus oil: An alternative to synthetic pesticides against two major storage insects Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) of rice. Industrial Crops and Products 143:111849. https://doi.org/10.1016/j.indcrop.2019.111849 Al-Qaysi AMK, Al-Ouqaili MTS, Al-Meani SAL (2020). Ciprofloxacin-and gentamicin-mediated inhibition of Pseudomonas aeruginosa biofilms is enhanced when combined the volatile oil from Eucalyptus camaldulensis. Systematic Reviews in Pharmacy 11:98-105. https://doi.org/10.31838/srp.2020.7.16 Al-Snafi PDAE (2017). The pharmacological and therapeutic importance of Eucalyptus species grown in Iraq. IOSR Journal of Pharmacy 7(3):72-91. https://doi.org/10.9790/3013-0703017291 Aleksic Sabo V, Knezevic P (2019). Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. Industrial Crops and Products 132:413-29. https://doi.org/10.1016/j.indcrop.2019.02.051 Almas I, Innocent E, Machumi F, Kisinza W (2018). Effect of geographical location on yield and chemical composition of essential oils from three Eucalyptus species growing in Tanzania. Asian Journal of Traditional Medicines 14(1):1-12. http://asianjtm.syphu.edu.cn/EN/ Ameur E, Sarra M, Yosra D, Mariem K, Nabil A, Lynen F, Larbi KM (2021). Chemical composition of essential oils of eight tunisian Eucalyptus species and their antibacterial activity against strains responsible for otitis. BMC Complementary Medicine and Therapies 21(1):1-16. https://doi.org/10.1186/s12906-021-03412-0 Ashour HM (2008). Antibacterial, antifungal, and anticancer activities of volatile oils and extracts from stems, leaves, and flowers of Eucalyptus sideroxylon and Eucalyptus torquata. Cancer Biology & Therapy 7(3):399-403. https://doi.org/10.4161/cbt.7.3.5367 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 14 Balti H, Abassi M, Dietz KJ, Kumar V (2021). Differences in ionic, enzymatic, and photosynthetic features characterize distinct salt tolerance in Eucalyptus species. Plants 10(7):1401. https://doi.org/10.3390/plants10071401 Baranska M, Schulz H, Reitzenstein S, Uhlemann U, Strehle MA, Krüger H, … Popp J (2005). Vibrational spectroscopic studies to acquire a quality control method of Eucalyptus essential oils. Biopolymers: Original Research on Biomolecules 78(5):237-248. https://doi.org/10.1002/bip.20284 Barbosa LCA, Filomeno CA, Teixeira RR (2016). Chemical variability and biological activities of Eucalyptus Spp. essential oils. Molecules 21(12):1671. https://doi.org/10.3390/molecules21121671 Bardaweel S, Hudaib M, Tawaha K (2014). Evaluation of antibacterial, antifungal, and anticancer activities of essential oils from six species of Eucalyptus. Journal of Essential Oil Bearing Plants 17(6):1165-74. https://doi.org/10.1080/0972060X.2014.963169 Bardaweel SK, Hudaib MM, Tawaha KA, Bashatwah RM, Material AP (2015). Antimicrobial, antioxidant and Free Radical Scavenging activities of essential oils extracted from six Eucalyptus species. International Journal of Biotechnology and Bioengineering 9(1):66-69. https://doi.org/10.5281/zenodo.1099314 Batish DR, Singh HP, Kohli RK, Kaur S (2008). Eucalyptus essential oil as a natural pesticide. Forest Ecology and Management 256(12):2166-74. https://doi.org/10.1016/j.foreco.2008.08.008 Ben Hassine D, Ben Ismail H, Jribi C, Khouja ML, Abderrabba M (2010). Chemical composition of some Tunisian Eucalyptus essential oils as obtained by hydrodistillation using Clevenger type apparatus. Biosciences Biotechnology Research Asia 7(2):647-665. Bhuyan DJ, Vuong QV, Chalmers AC, Van Altena IA, Bowyer MC, Scarlett CJ (2017). Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves. South African Journal of Botany 112:180-85. https://doi.org/10.1016/j.sajb.2017.05.030 Bignell CM, Dunlop PJ, Brophy JJ (1997a). Volatile leaf oils of some Queensland and Northern Australian species of the genus Eucalyptus. (Series Ii). Part i. Subgenus Symphyomyrtus, Section Adnataria:(A) Series Oliganthae,(b) Series Ochrophloiae,(c) Series Moluccanae,(d) Series Polyanthemae,(e) Se. Flavour and Fragrance Journal 12(1):19-27. https://doi.org/10.1002/(SICI)1099-1026(199701)12:1<19::AID-FFJ597>3.0.CO;2-F Bignell CM, Dunlop PJ, Brophy JJ (1997b). Volatile leaf oils of some Queensland and Northern Australian species of the genus Eucalyptus (Series II). Part II. Subgenera (a) Blakella,(b) Corymbia,(c) Unnamed,(d) Idiogenes,(e) Monocalyptus and (f) Symphyomyrtus. Flavour and Fragrance Journal 12(4):277-84. https://doi.org/10.1002/(SICI)1099-1026(199707)12:4<277::AID-FFJ642>3.0.CO;2-B Boland DJ, Brophy JJ, House APN (1991). Eucalyptus leaf oils: use, chemistry, distillation and marketing. Phytochemistry 31(6):2193. https://doi.org/10.1016/0031-9422(92)80403-2 Bouhlali EDT, Derouich M, Ben-Amar H, Meziani R, Essarioui A (2020). Exploring the potential of using bioactive plant products in the management of Fusarium oxysporum f. Sp. Albedinis: The causal agent of Bayoud disease on Date Palm (Phoenix dactylifera L.). Beni-Suef University Journal of Basic and Applied Sciences 9(1):1-9. https://doi.org/10.1186/s43088-020-00071-x Bouhlali EDT, Derouich M, Meziani R, Essarioui A (2021). Antifungal potential of phytochemicals against Mauginiella scaettae, the plant pathogen causing inflorescence rot of Date Palm. Scientifica 2021:1896015. https://doi.org/10.1155/2021/1896015 Bowyer BC, Jefferies PR (1959). Studies in plant chemistry: The Essential oils of Eucalyptus caesia Benth. and E. torquata Luehm. and the structure of Torquatone. Australian Journal of Chemistry 12(3):442-446. https://doi.org/10.1071/CH9590442 Chamali S, Ben Daoud H, Saadaoui E, Elfalleh W, Romdhane M (2019). A new process for the green extraction of essential oil from Eucalyptus oleosa: Microwave-assisted hydrodistillation. Arabian Journal of Medicinal and Aromatic Plants 5(3):35-46. https://doi.org/10.48347/IMIST.PRSM/ajmapv5i3.18666 Chamali S, Bouajila J, Ben Daoud H, Camy S, Saadaoui E, Condoret JS, Romdhane M (2021). Variation in chemical composition and biological properties of two Tunisian Eucalyptus essential oils under three eco-friendly extraction techniques. Journal of Essential Oil Research 34(1):36-53. https://doi.org/10.1080/10412905.2021.2008029 Chandorkar N, Tambe S, Amin P, Madankar C (2021). A systematic and comprehensive review on current understanding of the pharmacological actions, molecular mechanisms, and clinical implications of the genus Eucalyptus. Phytomedicine Plus 1(4):100089. https://doi.org/10.1016/j.phyplu.2021.100089 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 15 Chen H, Jiang JG (2010). Osmotic adjustment and plant adaptation to environmental changes related to drought and salinity. Environmental Reviews 18(NA):309-319. https://doi.org/10.1139/A10-014 Yiğit Hanoğlu D, Hanoğlu A, Adediran SB, Baser KHC, Özkum Yavuz D (2022). The essential oil compositions of two Eucalyptus sp. (E. camaldulensis Dehnh. and E. torquata Luehm.) naturalized to Cyprus. Journal of Essential Oil Research. https://doi.org/10.1080/10412905.2022.2147592 Derwich E, Benziane Z, Boukir A (2009). GC/MS analysis of volatile constituents and antibacterial activity of the essential oil of the leaves of Eucalyptus globulus in Atlas median from Morocco. Advances in Natural and Applied Sciences 3(3):305-314. http://www.insipub.com/aensi/anas/2009/305-313.pdf Dhakad AK, Pandey VV, Beg S, Rawat JM, Singh A (2018). Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: A review. Journal of the Science of Food and Agriculture 98(3):833-848. https://doi.org/10.1002/jsfa.8600 Ebadollahi A, Naseri B, Abedi Z, Setzer WN, Changbunjong T (2022). Promising insecticidal efficiency of essential oils isolated from four cultivated Eucalyptus species in Iran against the lesser grain borer, Rhyzopertha dominica (F.). Insects 13(6):517. https://doi.org/10.3390/insects13060517 Ebadollahi A, Sendi JJ, Maroufpoor M, Rahimi-Nasrabadi M (2017). Acaricidal potentials of the terpene-rich essential oils of two Iranian Eucalyptus Species against Tetranychus urticae Koch. Journal of Oleo Science 66(3):307-314. https://doi.org/10.5650/jos.ess15258 Eisikowitch D, Dag A, Samocha Y (2012). Two Eucalypts and one hybrid as a potential source of pollen and nectar in Israel. American Bee Journal 152(6):607-609. El-Juhany L, Al Al-Shaikh AM (2015). Evaluation of the growth and adaptability of some exotic Acacia and Eucalyptus species to Riyadh Region, Saudi Arabia. Research Journal of Agriculture 2:1-12. El-juhany LI, Aref IM, Ahmed AIM (2008). Response of Eucalyptus camaldulensis, Eucalyptus microtheca and Eucalyptus intertexta seedlings to irrigation with saline water. World Journal of Agricultural Sciences 4:825-834. https://www.idosi.org/wjas/wjas4(s)/5.pdf Elaissi A, Medini H, Simmonds M, Lynen F, Farhat F, Chemli R, … Khouja ML (2010). Variation in volatile leaf oils of twelve Eucalyptus species harvested from Hajeb Layoun arboreta (Tunisia). Chemistry & Biodiversity 7(3):705- 716. https://doi.org/10.1002/cbdv.201000103 Elaissi A, Medini H, Simmonds M, Lynen F, Farhat F, Chemli R, … Khouja ML (2011a). Variation in volatile leaf oils of five Eucalyptus species harvested from Jbel Abderrahman arboreta (Tunisia). Chemistry & Biodiversity 8(2):352- 361. https://doi.org/10.1002/cbdv.201000103 Elaissi A, Medini H, Simmonds M, Lynen F, Farhat F, Chemli R, … Khouja ML (2011b). Variation in volatile leaf oils of seven Eucalyptus species harvested from Zerniza arboreta (Tunisia). Chemistry & Biodiversity 8(2):362-372. https://doi.org/10.1002/cbdv.201000103 Elaissi A, Rouis Z, Mabrouk S, Bel Haj Salah K, Aouni M, Khouja ML, … Harzallah-Skhiri F. (2012). Correlation between chemical composition and antibacterial activity of essential oils from fifteen Eucalyptus species growing in the Korbous and Jbel Abderrahman arboreta (North East Tunisia). Molecules 17(3):3044-3057. https://doi.org/10.1002/cbdv.201000103 Elaissi A, Rouis Z, Salem NAB, Mabrouk S, Ben Salem Y, Salah KBH, … Khouja ML (2012). Chemical composition of eight Eucalyptus species essential oils and the evaluation of their antibacterial, antifungal and antiviral activities. BMC Complementary and Alternative Medicine 12(1):1-15. https://doi.org/10.1186/1472-6882-12-81 Elaissi A, Salah KH, Mabrouk S, Larbi KM, Chemli R, Harzallah-Skhiri F (2011). Antibacterial activity and chemical composition of twenty Eucalyptus species essential oils. Food Chemistry 129(4):1427-1434. https://doi.org/10.1016/j.foodchem.2011.05.100 Elansary HO, Salem MZM, Ashmawy NA, Yessoufou K, El-Settawy AAA (2017). In vitro antibacterial, antifungal and antioxidant activities of Eucalyptus Spp. leaf extracts related to phenolic composition. Natural Product Research 31(24):2927-2930. https://doi.org/10.1080/14786419.2017.1303698 El Finti A, El Boullani R, Zahidi A, El Mousadik A (2022). Management of the Prickly Pear Mealy Bug, Dactylopius opuntiae using bio-insecticide in Morocco. Advances in Entomology 10(4):267-74. https://doi.org/10.4236/ae.2022.104019 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 16 Gakuubi MM, Maina AW, Wagacha JM (2017). Antifungal activity of essential oil of Eucalyptus camaldulensis Dehnh. against selected Fusarium Spp. International Journal of Microbiology 2017:8761610. https://doi.org/10.1155/2017/8761610 Gallon C, Martello RH, Cozzer G, Rezende CAL, Calisto JFF, Floss PA, … Albeny-Simões D (2020). Chemistry matters: Biological activity of Eucalyptus essential oils on Mosquito larval mortality. Entomologia Experimentalis et Applicata 168(5):407-415. https://doi.org/10.1111/eea.12908 Ghaffar A, Yameen M, Kiran S, Kamal S, Jalal F, Munir B, … Jabbar A (2015). Chemical composition and in-vitro evaluation of the antimicrobial and antioxidant activities of essential oils extracted from seven Eucalyptus species. Molecules 20(11):20487-20498. https://doi.org/10.3390/molecules201119706 Ghisalberti EL (1996). Bioactive Acylphloroglucinol derivatives from Eucalyptus species. Phytochemistry 41(1):7-22. https://doi.org/10.1016/0031-9422(95)00484-X Gilles M, Zhao J, An M, Agboola S (2010). Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chemistry 119(2):731-737. https://doi.org/10.1016/j.foodchem.2009.07.021 Goldbeck JC, do Nascimento JE, Jacob RG, Fiorentini ÂM, da Silva WP (2014). Bioactivity of essential oils from Eucalyptus globulus and Eucalyptus urograndis against planktonic cells and biofilms of Streptococcus mutans. Industrial Crops and Products 60:304-309. https://doi.org/10.1016/j.indcrop.2014.05.030 Harkat-Madouri L, Asma B, Madani K, Bey-Ould Si Said Z, Rigou P, Grenier D, … Boulekbache-Makhlouf L (2015). Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Industrial Crops and Products 78:148-153. https://doi.org/10.1016/j.indcrop.2015.10.015 Ben Hassine D, Ben Ismail H, Jribi C, Khouja ML, Abderrabba M (2016). Chemical composition of some Tunisian Eucalyptus essential oils as obtained by hydrodistillation using Clevenger type apparatus. Biosciences Biotechnology Research Asia 7(2):647-656. https://www.biotech-asia.org/?p=8997 Herzi N, Bouajila J, Camy S, Cazaux S, Romdhane M, Condoret JS (2013). Comparison between Supercritical CO2 extraction and hydrodistillation for two species of Eucalyptus: Yield, chemical composition, and antioxidant activity. Journal of Food Science 78(5):67-72. https://doi.org/10.1111/1750-3841.12113 Ishnava KB, Chauhan JB, Barad MB (2013). Anticariogenic and phytochemical evaluation of Eucalyptus globules Labill. Saudi Journal of Biological Sciences 20(1):69-74. https://doi.org/10.1016/j.sjbs.2012.11.003 Khouja ML, Khaldi A, Rejeb MN (2001). Results of the Eucalyptus introduction trials inTunisia. Proceeding of the international conference on Eucalyptus in the Mediterranean basin: Perspectives and new utilization. Centro Promozione Pubblicità, Florence, Taormina-Italy: pp 163-168. Knezevic P, Aleksic V, Simin N, Svircev E, Petrovic A, Mimica-Dukic N (2016). Antimicrobial activity of Eucalyptus camaldulensis essential oils and their interactions with conventional antimicrobial agents against multi-drug resistant Acinetobacter baumannii. Journal of Ethnopharmacology 178:125-136. https://doi.org/10.1016/j.jep.2015.12.008 Lahmadi G, Lahmar A, Znati M, Elaieb MT, Khouja ML, Ascrizzi R, … Jannet HB (2021). Chemical composition and cytotoxic activity of Eucalyptus torquata Luehm. and Eucalyptus salmonophloia F. Muell. Tru. Chemistry & Biodiversity 18(11):2100315. https://doi.org/10.1016/j.jep.2015.12.008 Li C, Wang K (2003). Differences in drought responses of three contrasting Eucalyptus microtheca F. Muell. populations. Forest Ecology and Management 179(1-3):377-385. https://doi.org/10.1016/S0378-1127(02)00552-2 Limam H, Ben Jemaa M, Tammar S, Ksibi N, Khammassi S, Jallouli S, … Msaada K (2020). Variation in chemical profile of leaves essential oils from thirteen Tunisian Eucalyptus species and evaluation of their antioxidant and antibacterial properties. Industrial Crops and Products 158:112964. https://doi.org/10.1016/j.indcrop.2020.112964 Luís Â, Duarte A, Gominho J, Domingues F, Duarte AP (2016). Chemical composition, antioxidant, antibacterial and Anti-Quorum sensing activities of Eucalyptus globulus and Eucalyptus radiata essential oils. Industrial Crops and Products 79:274-282. https://doi.org/10.1016/j.indcrop.2015.10.055 Madsen PA, Mulligan DR (2006). Effect of NaCl on emergence and growth of a range of provenances of Eucalyptus citriodora, Eucalyptus populnea, Eucalyptus camaldulensis and Acacia salicina. Forest Ecology and Management 228(1-3):152-159. https://doi.org/10.1016/j.foreco.2006.02.044 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 17 Marzoug HNB, Romdhane M, Lebrihi A, Lebrihi F, Couderc F, Abderraba M, … Bouajila J (2011). Eucalyptus oleosa essential oils: Chemical composition and antimicrobial and antioxidant activities of the oils from different plant parts (stems, leaves, flowers and fruits). Molecules 16(2):1695-1709. https://doi.org/10.3390/molecules16021695 May FE, Ash JE (1990). An assessment of the allelopathic potential of Eucalyptus. Australian Journal of Botany 38(3):245- 254 .https://doi.org/10.1071/BT9900245 Mechergui K, Jaouadi W, Naghmouchi S, Alsubeie M, Khouja ML (2019). Micromorphological observation of Eucalyptus seeds, multivariate statistical analyses and modeling of their germination under salt stress and osmotic constraint. Cerne 25:156-171. https://doi.org/10.1590/01047760201925022635 Mossi AJ, Astolfi V, Kubiak G, Lerin L, Zanella C, Toniazzo G, … Restello R (2011). Insecticidal and repellency activity of essential oil of Eucalyptus Sp. against Sitophilus zeamais Motschulsky (Coleoptera, Curculionidae). Journal of the Science of Food and Agriculture 91(2): 273-277. https://doi.org/10.1002/jsfa.4181 Mulyaningsih S, Sporer F, Reichling J, Wink M (2011). Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharmaceutical Biology 49(9):893-899. https://doi.org/10.3109/13880209.2011.553625 Mulyaningsih S, Sporer F, Zimmermann S, Reichling J, Wink M (2010). Synergistic properties of the terpenoids Aromadendrene and 1,8-Cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine 17(13):1061-1066. https://doi.org/10.1016/j.phymed.2010.06.018 Najum Rasheed A, Muhammad Asif N, Umer R (2005). Chemical compositional and intra provenance variation for content of essential oil in Eucalyptus crebra. Asian Journal of Plant Sciences 4(5):519-523. https://doi.org/10.3923/ajps.2005.519.523 Nasim M, Qureshi RH, Aziz T, Saqib M, Nawaz S, Sahi ST, Pervaiz S (2008). Growth and ionic composition of salt stressed Eucalyptus camaldulensis and Eucalyptus teretcornis. Pakistan Journal of Botany 40(2):799-805. http://173.208.131.244:9060/xmlui/handle/123456789/17120 Nikbakht MR, Rahimi-Nasrabadi M, Ahmadi F, Gandomi H, Abbaszadeh S, Batooli H (2015). The chemical composition and in vitro antifungal activities of essential oils of five Eucalyptus species. Journal of Essential Oil Bearing Plants 8(3):666-677. https://doi.org/10.1080/0972060X.2014.935061 Oerke EC (2006). Crop losses to pests. The Journal of Agricultural Science 144(1):31-43. https://doi.org/10.1017/S0021859605005708 Ogunwande IA, Olawore NO, Adeleke KA, Konig WA (2003). Chemical composition of the essential oils from the leaves of three Eucalyptus species growing in Nigeria. Journal of Essential Oil Research 15(5):297-301. https://doi.org/10.1080/10412905.2003.9698595 Palá-Paúl J, Pérez-Alonso MJ, Velasco-Negueruela A, Sanz J (2002). Study of essential oils of Eucalyptus resinifera Smith, E. tereticornis Smith and Corymbia Maculata (Hook.) grown in Cuba. Flavour and Fragrance Journal 17(1):1-4. https://doi.org/10.1002/ffj.1026 Pimentel D, Acquay H, Biltonen M, Rice P, Silva M, Nelson J, … D’Amore M (1992). Environmental and economic costs of pesticide use. BioScience 42(10):750-760. https://doi.org/10.2307/1311994 Radwan SM, Zidan ZH, El-Hammady A, Aly MM (2000). Field performance of tested Eucalyptus plant extracts, biocides and conventional pesticides against key pests infesting cotton in Egypt. Annals of Agricultural Science (Cairo) 45(2): 777-791. https://www.cabdirect.org/cabdirect/abstract/20013015331 Raja N (2014). Botanicals: Sources for eco-friendly biopesticides. Journal of Biofertilizers and Biopesticides 5(1):1. https://doi.org/10.4172/2155-6202.1000e122 Saadaoui E, Ben Yahia K, Dhahri S, Ben Jamaa ML, Khouja ML (2017). An overview of adaptative responses to drought stress in Eucalyptus spp. Forestry Studies 67(1):86. https://doi.org/10.1515/fsmu-2017-0014 Saadaoui E, Ben Yahia K, Chemlali I, Belaïd S, Ben Romdhane C (2022). Eucalypt in the Tunisian arid region: Diversity and valorization for honey production. International Journal of Agriculture and Nutrition 4(1):1-5. Sadraoui-Ajmi I, Benali N, Soltani A, Chaib S, Limem E, Jallouli S, … Mediouni-Ben Jemâa J (2022). Usage of agricultural DAP-Fertilizer and Eucalyptus essential oils as potential attractants against the Mediterranean fruit fly Ceratitis capitata (Tephritidae). Journal of Asia-Pacific Entomology 25(1):101857. https://doi.org/10.1016/j.aspen.2021.101857 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 18 Salehi B, Sharifi-Rad J, Quispe C, Llaique H, Villalobos M, Smeriglio A, … Martins N (2019). Insights into Eucalyptus genus chemical constituents, biological activities and health-promoting effects. Trends in Food Science & Technology 91:609-624. https://doi.org/10.1016/j.tifs.2019.08.003 Salem N, Kefi S, Tabben O, Ayed A, Jallouli S, Feres N, … Elkahoui S (2018). Variation in chemical composition of Eucalyptus globulus essential oil under phenological stages and evidence synergism with antimicrobial standards. Industrial Crops and Products 124:115-125. https://doi.org/10.1016/j.indcrop.2018.07.051 Sanders GJ, Arndt SK (2012). Osmotic adjustment under drought conditions: Plant responses to drought stress: from morphological to molecular features. Springer, Berlin Heidelberg pp 199-229. https://doi.org/10.1007/978-3-642- 32653-0-8 Scazzocchio F, Garzoli S, Conti C, Leone C, Renaioli C, Pepi F, Angiolella L (2016). Properties and limits of some essential oils: Chemical characterisation, antimicrobial activity, interaction with antibiotics and cytotoxicity. Natural Product Research 30(17):1909-1918. https://doi.org/10.1080/14786419.2015.1086346 Sebei K, Sakouhi F, Herchi W, Khouja ML, Boukhchina S (2015). Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves. Biological Research 48(1):1-5. https://doi.org/10.1186/0717-6287- 48-7 Sefidkon F, Assareh MH, Abravesh Z, Barazandeh MM (2010). Chemical composition of the essential oils of four cultivated Eucalyptus species in iran as medicinal plants (E. microtheca, E. spathulata, E. largiflorens and E. torquata). Iranian Journal of Pharmaceutical Research 2:135-140. https://doi.org/10.22037/ijpr.2010.711 Sefidkon F, Bahmanzadegan A, Assareh MH (2008). Effect of distillation methods and harvesting times on the essential oil and cineole content of Eucalyptus dealbata. Chemistry of Natural Compounds 44(2):250-253. https://doi.org/10.1080/0972060X.2008.10643626 shariat A, Assareh MH (2016). Physiological and biochemical responses of eight Eucalyptus species to salinity stress. Ecopersia 4(1):1269-1282. https://doi.org/10.18869/modares.ecopersia.4.1.1269 Silva J, Abebe W, Sousa SM, Duarte VG, Machado MIL, Matos FJA (2003). Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. Journal of Ethnopharmacology 89(2-3):277-283. https://doi.org/10.1016/j.jep.2003.09.007 Silva SM, Abe SY, Murakami FS, Frensch G, Marques FA, Nakashima T (2011). Essential oils from different plant parts of Eucalyptus cinerea F. Muell. Ex Benth. (Myrtaceae) as a source of 1,8-Cineole and their bioactivities. Pharmaceuticals 4(12):1535-1550. https://doi.org/10.3390/ph4121535 Singh IP, Sidana J, Bansal P, Foley WJ (2009). Phloroglucinol compounds of therapeutic interest: Global patent and technology status. Expert Opinion on Therapeutic Patents 19(6):847-866. https://doi.org/10.1517/13543770902916614 Siramon P, Ohtani Y (2007). Antioxidative and antiradical activities of Eucalyptus camaldulensis leaf oils from Thailand. Journal of Wood Science 53(6):498-504. https://doi.org/10.1007/s10086-007-0887-7 Souden S, Ennajeh M, Ouledali S, Massoudi N, Cochard H and Khemira H (2020). Water relations, photosynthesis, xylem embolism and accumulation of carbohydrates and cyclitols in two Eucalyptus species (E. camaldulensis and E. torquata) subjected to dehydration–rehydration cycle. Trees 34(6):1439-1452. https://doi.org/10.1007/s00468-020-02016-4 Teulieres C, Bossinger G, Moran G, Marque C (2007). Stress studies in Eucalyptus. Plant Stress 1(2):197-215. Üstüner T, Kordali Ş, Bozhüyük AU, Kesdek M (2018). Investigation of pesticidal activities of essential oil of Eucalyptus camaldulensis Dehnh. Records of Natural Products 12(6):557-568. https://doi.org/10.25135/rnp.64.18.02.088 Vuong QV, Chalmers AC, Jyoti Bhuyan D, Bowyer MC, Scarlett CJ (2015). Botanical, phytochemical, and anticancer properties of the Eucalyptus species. Chemistry & Biodiversity 12(6):907-924. https://doi.org/10.1002/cbdv.201400327 White DA, Turner NC, Galbraith JH (2000). Leaf water relations and stomatal behavior of four allopatric Eucalyptus species planted in Mediterranean southwestern Australia. Tree Physiology 20(17):1157-1165. https://doi.org/10.1093/treephys/20.17.1157 Wilkinson JM, Cavanagh HMA (2005). Antibacterial activity of essential oils from Australian native plants. Phytotherapy Research 19(7):643-646. https://doi.org/10.1002/ptr.1716 Ben Rabeh S et al. (2023). Not Sci Biol 15(2):11502 19 Yao ZW, Liu H, Zhou R, Feng MY, Wang F, Qin XJ, … Zheng YT (2021). Non-volatile Acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5. Bioorganic Chemistry 116:105303. https://doi.org/10.1016/j.bioorg.2021.105303 Yiğit Hanoğlu D, Hanoğlu A, Adediran SB, Baser KHC, Özkum Yavuz D (2022). The essential oil compositions of two Eucalyptus sp. (E. camaldulensis Dehnh. and E. torquata Luehm.) naturalized to Cyprus. Journal of Essential Oil Research. https://doi.org/10.1080/10412905.2022.2147592 Zandi-Sohani N, Ramezani L (2015). Evaluation of five essential oils as botanical acaricides against the strawberry spider mite Tetranychus turkestani Ugarov and Nikolskii. International Biodeterioration & Biodegradation 98:101-106. https://doi.org/10.1016/j.ibiod.2014.12.007 Zhang J, An M, Wu H, Stanton R, Lemerle D (2010). Chemistry and bioactivity of Eucalyptus essential oils. Allelopathy Journal 25(2):313-330. Zhou LJ, Li FR, Huang LJ, Yang ZR, Yuan S, Bai LH (2016). Antifungal activity of Eucalyptus oil against Rice Blast fungi and the possible mechanism of gene expression pattern. Molecules 21(5):621. https://doi.org/10.3390/molecules21050621 Zhu JK (2001). Plant salt tolerance. Trends in Plant Science 6(2):66-71. https://doi.org/10.1016/S1360-1385(00)01838- 0 Zrira S, Khiranr FEL, Benjllalr B (1994). Huiles essentielles de six espèces xérophyles d’Eucalyptus: Effet du milieu sur les rendements et la composition chimique [Essential oils of six xerophilic Eucalyptus species: effect of location on the yield and the chemical composition]. Revue Marocaine des Sciences Agronomiques et Vétérinaires 14(1):5-9. The journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work. 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