90 Nova Biotechnologica et Chimica 15-1 (2016) DOI 10.1515/nbec-2016-0010 © University of SS. Cyril and Methodius in Trnava DEGRADATION OF SYNTHETIC DYES BY LACCASES – A MINI-REVIEW BARBORA LEGERSKÁ, DANIELA CHMELOVÁ, MIROSLAV ONDREJOVIČ Department of Biotechnologies, Faculty of Natural Sciences, University of SS. Cyril and Methodius, Nam.J. Herdu 2, Trnava, SK-917 01, Slovak Republic (daniela.chmelova@ucm.sk) Abstract: Laccases provide a promising future as a tool to be used in the field of biodegradation of synthetic dyes with different chemical structures. These enzymes are able to oxidize a wide range of phenolic substrates without the presence of additional co-factors. Laccases have been confirmed for their potential of synthetic dye degradation from wastewater and degradation products of these enzymatic reactions become less toxic than selected dyes. This study discusses the potential of laccase enzymes as agents for laccase-catalyzed degradation in terms of biodegradation efficiency of synthetic dyes, specifically: azo dyes, triphenylmethane, indigo and anthraquinone dyes. Review also summarizes the laccase-catalyzed degradation mechanisms of the selected synthetic dyes, as well as the degradation products and the toxicity of the dyes and their degradation products. Key words: laccase, synthetic dye, biodegradation, toxicity, degradation products. 1. Introduction Synthetic dyes have a nearly exhaustible range of application in various types of industry including food, pharmaceutical, textile, printing, paper or chemical. It has been estimated that approximately 5-10 % of dyes used in the industrial sector could remain persistent in wastewater (DAFALE et al., 2008). Untreated dyeing effluents are a serious environmental problem in the twenty first century. The release of dyes into the environment is harmful due to toxicity, carcinogenic and/or mutagenic effects on living organisms. The presence of synthetic dyes in wastewater can cause an increase of BOD (biochemical oxygen demand) and COD (chemical oxygen demand) levels. Moreover, the chromophoric groups strongly absorb sunlight and therefore, photosynthetic activity of organisms is inhibited (DA SILVA et al., 2010; KAGALKAR et al., 2010). The negative impact of synthetic dyeshas been observed in the oestrous cycle and reproductive system in rats (NATH et al., 2015), in biochemical markers of vital organs, such as the liver and kidney (AMIN et al., 2010) and to foetal growth (WAN et al., 2011; GOPINATHAN et al., 2015). A correlation between the presence of synthetic dyes and high clastogenic activity in bone marrow cells has been observed in rats (RAJAGURU et al., 1999). Additional studies have shown that a correlation exists between synthetics dyes and mitotic abnormalities (AZMI et al., 1998), as well as cirrhosis from chronic consumption of selected dyes (AXON et al., 2012). Because of the aforementioned reasons, wastewater treatment containing synthetic dyes is a global problem requiring an immediate yet cost-effective solution. Degradation of synthetic dyes using biological methods is a promising, environmental- friendly process and these methods are presented as a cheaper alternative to the Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 91 expensive physicochemical methods producing large amount of sludge, which must be degraded by other processes. Using biological methods, the degradation of synthetic dyes can occur in a cost efficient, eco-friendly format with certain advantages. Not only are the products of enzyme-catalyzed reaction less toxic than synthetic dyes (CAMPOS et al., 2001; TELKE et al., 2011; ADNAN et al., 2015), but the degradation products can be utilized by various natural organisms. These biological methods are founded on enzymes, which are produced by organisms. In the case of enzymatic degradation, azoreductases, peroxidases and phenol oxidases have a potential for dye biodegradation, but in certain aspects each of these enzyme falters in their biodegradation efficiency. Azoreductases (EC 1.7.1.6) require additional co-factors such as NADH2, NADPH2 and FADH2 for the activation of the catalyst. Additionally, azoreductases can degrade only azo dyes via reductive cleavage of azo bonds (PANDEY et al., 2007). One of the main disadvantages of this enzyme-catalyzed degradation processes the formation of toxic products (PLATZEK et al., 1999). It is known that azoreductases are intracellular enzymes and their use in the pure form without production organisms is problematic due to issues of stability and necessary regeneration of co-factors. Furthermore, the direct application of microorganism produced azoreductases can result in additional problems from the inability of dye diffusion through cell membranes, due to the molecular weight of azo dyes (ROBINSON et al., 2001). Peroxidases (EC 1.11.1.X), such as horseradish peroxidase, chloroperoxidases, lignin peroxidases and manganese peroxidases, are other enzymes that could be useful for synthetic dye biodegradation but have a factor that limit their benefits in synthetic dye degradation. All of these enzymes belong to hemoproteins which catalyse the chemical reactions in the presence of hydrogen peroxide (DURAN et al., 2002). These reactions require specialized attention from because the presence of hydrogen peroxide at too high of a concentration can cause the inactivation of peroxidases (AITKEN et al., 1994). The last enzyme group described in the literature as useful for synthetic dye decolorization includes phenoloxidases. These enzymes belong to oxidases which catalyse the oxidation of phenolic compounds in the presence of oxygen without additional co-factors. One of these enzymes, laccase (EC 1.10.3.2), is a member of the multicopper oxidase family (TELKE et al., 2011). Laccases catalyse the removal of a hydrogen atom from the hydroxyl group via electron oxidation (BOLLAG, 1992), which generates non-toxic products during synthetic dye biodegradation (CAMPOS et al., 2001; TELKE et al., 2010; ADNAN et al., 2015). Although the biodegradation potential of laccases is mostly evaluated by the change of a absorption spectrum of dye (SUZUKI et al., 2001; BIBI et al., 2011; CHMELOVÁ and ONDREJOVIČ, 2015), the destruction of chromophores can led to dye degradation (CHEN and TING, 2015). In this mini-review, the synthetic dye degradation of laccase-catalyze reaction has been observed with regards to their degradation products and their potential toxicity. 2. Laccases Laccases (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductases) are classified as phenol oxidases which are able to catalyse one-electron oxidation of the substrate associated with the simultaneous reduction of oxygen in water. These enzymes are Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 92 Legerská et al. produced by a wide spectrum of organisms such as bacteria, filamentous fungi or plants (RODRÍGUEZ-COUTO and HERRERA, 2006; BIBI et al., 2011; ZHOU and XIANG, 2013). Laccases are monomeric, dimeric or tetrameric glycoproteins with four copper atoms per monomer located in catalytic sites. Type 1 copper (Cu T1, ligated by at least one Cys and two His) is paramagnetic and responsible for the characteristic blue colour and the oxidation of the substrate. Type 2 copper (Cu T2, ligated by two His) and two copper atoms of type 3 (Cu T3, each ligated by three His) conforming trinuclear cluster play a key role in the reduction of molecular oxygen to two molecules of water (DAVIES and DUCROS, 2006; MOT and SILAGHI- DUMITRESCU, 2012). Laccase can oxidize different organic compounds such as mono- and di-phenols or their derivatives with hydroxy-, carboxy-, methoxy-, amino- or sulpho- functional groups by radical mechanism. Phenols are typical substrates for laccases due to their low redox potential which allows for electron abstraction by the Cu T1. Therefore, the ability for laccase enzymes to oxidize molecules is determined by the redox potential of Cu T1 (E0 Cu T1). E0 values have been determined using potentiometric titrations for various laccases (Table 1). According to E0 Cu T1, the laccases can be classified into three categories: low-, medium- and high-redox potential laccases. This redox potential is a result of combined factors such as copper-ligand interactions, the effect of desolvation around the T1 site, the intermolecular electrostatic interactions, and the restrictions in protein folding (LI et al., 2004). Bacterial and plant laccases have low- redox potential (below +460 mV vs. NHE – normal hydrogen electrode) while fungal laccases belong to medium- and high-redox potential laccases (MATE and ALCALDE, 2015). Laccases with medium-redox potential (+460 to +710 mV vs. NHE) are produced mainly by ascomycetes and basidiomycetes. Laccases with high- redox potential (+730 to +790 mV vs. NHE) are commonly found in white-rot fungi (basidiomycetes). For industrial application, laccases with a high-redox potential are the most relevant and most useful. The efficiency of synthetic dye oxidation by laccases depends on differences between the redox potential of dye and potential of enzyme (YANG et al., 2015). For phenolic substrate, the reaction starts with the deprotonation of the phenolic hydroxyl group. This results in the formulation of unstable phenoxy radicals which lead to quinone formation. This process combines the oxidative transformation without the coupling of forming products, where oxidation cleavage leads to a reduction of product molecular mass and oxidative decarboxylation of different products (POLAK and JAROSZ-WILKOLAZKA, 2012). For diversification of substrate specificity, laccases need the presence of low- molecular weight compounds (redox mediators) because they usually are not able to oxidize non-phenolic compounds or molecules with high redox potential (GIARDINA et al., 2010). These compounds act as intermediate substrates for laccases. The initial step is the oxidation of a redox mediator to forming a radical (1) (FABBRINI et al., 2002). The substrate is attacked the radical thereby giving rise to product and producing mediator back. Med-OH laccase Med-OH Med-O (1) Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 93 Table 1. The values of redox potential E0 (Cu T1) for laccases isolated from various organisms. Organism MW (kDa) pH E 0 (Cu T1) (mV vs. NHE) Reference Rhus vernicifera - 5.5-8.5 0.410 JOHNSON et al., 2003 Trametes ochracea 64±2 3.7- 4.9 0.79±0.1 SHLEEV et al., 2004; SHLEEV et al., 2005 Trametes hirsuta 70±2 3.5-4.5 0.78±0.1 SHLEEV et al., 2004; SHLEEV et al., 2005 Coprinus cinereus 58 5.5 0.55 SCHNEIDER et al., 1999 Cerrena maxima 67±4 4.0-6.0 0.75±0.05 SHLEEV et al., 2004; SHLEEV et al., 2005 Coriolopsis fulvocinerea 65±2 3.9-5.2 0.78±0.1 SHLEEV et al., 2004; SHLEEV et al., 2005 Ganoderma sp. 62 3.0-5.0 0.63 SHARMA et al., 2013 Marasmius guercophilus C30 65 5.7 0.73 KLONOWSKA et al., 2002 Melanocarpus alomyces - 8.0 0.46±0.01 KRUUS et al., 2003 Pleurotus ostreatus - 3.0 0.588 DAI et al., 2016 Pycnoporus sanguineus 67 4.5 0.747 ZIMBARDI et al., 2016 Redox mediators can be natural or synthetic.3-hydroxyanthranilic acid, 4-hydroxybenzoic acid, 4-hydroxybenzyl alcohol, syringaldehyde, sinapinic acid, acetovanillon, ferulic acid, vanillin, p-coumaric acid (MOREIRA et al., 2014) belong to the group of natural mediators. Generally these compounds are phenolic substrates produced by white-rot fungi. Synthetic redox mediators are 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), 1-hydroxybenzotriazole, N-hydroxyphthalimide, violuric acid or N-hydroxyacetanilide. The application of artificial mediators has several disadvantages such as high cost and toxicity (CANAS and CAMARERO, 2010). Laccases are able to degrade various organic pollutants with different chemical structures via direct oxidation (BOLLAG, 1992). This ability depends on the enzyme as well as dye structures. The presence and the location of different functional groups in dye structure can affect degradation of selected dyes. For most laccases, the presence of orto- and meta- substituents in dye structure is preferable for laccase oxidation as these in para-position (BALDRIAN, 2006; TAUBER et al., 2008). Electron-donating substituents (-OH, -CH3, -NH2, -N(CH3)2) contribute to increased biodegradability while electron-withdrawing substituents (-COOH, -SO3H, -NO2, -Cl, -Br) make a ring less susceptible to biological oxidation (SUZUKI et al., 2001). For example CHIVUKULA and RENGANATHAN (1995) found that laccase from Pyricularia oryzae oxidizes only azo dyes with methyl- or methoxy- substituents in azo structure while non-substituted 4-(4´-sulfophenylazo)-phenol and its 2-chloro- and 2-nitro-analogs were not oxidized. The negative effect of halogen groups (-Cl, -Br) in the structure of triphenylmethane dyes such as Bromophenol Blue and Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 94 Legerská et al. A B C D Bromochlorophenol Blue to their decolorization by laccase was confirmed also in our recent work (CHMELOVÁ and ONDREJOVIČ, 2015). 3. Synthetic dyes Synthetic dyes are complex aromatic compounds which are produced via chemical synthesis which provide a wide range of colours. The most industrially used synthetic dyes can be classified as azo dyes, anthraquinone, triphenylmethane and indigo dyes (Fig. 1) according to the main structure which often includes chromophores (-C=C-, -C=O, -C=N-, -NO2, -N=N- and quinonoid rings) responsible for absorption of visible light. N N NH2 NH2 O O OH OH NN CH3 H3C CH3 CH3 H N N H O O Fig. 1. The basic structure of selected synthetic dyes. A – azo dye (Chrysoidine), B – anthraquinone (Alizarine), C – triphenylmethane (Malachite Green) a D – indigo dye (Indigo). Diversion of compounds belonging to individual synthetic dye groups is caused mainly by the auxochrome groups e.g. (CH3)2N-, -NH2, -OH, -OCH3, CH3CO-, CH3-, - NO2, -SO3H, -COR, -CO2H, halogens (FORGACS et al., 2004). 3.1 Mechanisms of azo dye degradation Azo dyes belong to the group of aromatic compounds which contain one or more azo bonds (-N=N-). Azo dyes are produced by the copulation of diazonium salts with amines or phenols or naphtol, respectively. Azo bonds are substituted with benzene or naphthalene which can contain different functional groups such as -Cl, -CH3, -NO2, -NH2, -OH and -CO. In the enzymatic degradation pathway, azo dyes can be cleaved symmetrically or asymmetrically (TELKE et al., 2009) through a highly non-specific free radical mechanism, forming phenolic products. The biggest problem with enzymatic cleavage of azo dyes is the formation of toxic products, mainly amines. Therefore, it is important to identify and evaluate toxicity and/or mutagenicity of degradation products (CHHABRA et al., 2009). Azo dye degradation by laccases starts by asymmetric cleavage of the azo bond followed by oxidative cleavage, desulfonation, deamination, demethylation and dihydroxylation, depending on dye structure (TELKE et al., 2009; TELKE, et al., 2011; ADNAN et al., 2015; YANG et al., 2015; ZHENG et al., 2016). Some authors describe degradation of azo dyes without the cleavage of the azo bond (CHEN, 2006; PEREIRA et al., 2009). This mechanism includes the formation of phenolic type compounds resulting from highly non-specific free radical mechanism (CHEN, 2006). The inability of laccase to cleave azo bond in the structure of dyes may be related to Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 95 the redox potential of laccase. PEREIRA et al. (2009) suggested the proposed mechanism for biotransformation of the mono azo dye Sudan Orange G by laccase from Bacillus subtilis. The enzymatic oxidation of Sudan Orange G results in the production of oligomers, and possibly polymers through radical coupling reactions without the cleavage of azo bond. Laccase from B. subtilis has a low-redox potential (455 mV vs. NHE) (DURAO et al., 2006), so it belongs to the group of low-potential laccases. On the other hand, laccases from fungal organisms are more applicable for degradation of azo dyes because they belong to the group of high-redox potential laccases (MATE and ALCALDE, 2015). An example can be found in the work of TELKE et al. (2010) which describe the degradation of azo dye (Methyl Orange) by laccases from Aspergillus ochraceus NCIM-1146 (Fig. 2). N NHO3S N(CH3)2 Asymetric cleavage Decolorization HN NHO3S N(CH3)2 p-hydroxybenzene sulfonic acid p-N,N'-dimethylamine phenyldiazine Fig. 2. Degradation mechanism of mono azo Methyl Orange by laccase from Aspergillus ochraceus (TELKE et al., 2010). The first step of mono azo dye decolorization by laccases is carbocation, the formation of an electron-deficient reaction centre and therefore highly reactive intermediates. These can be subject to the nucleophilic attack by -SO3, -OH or halogen nucleophiles resulting in asymmetric cleavage of azo bond (TELKE et al., 2010). Degradation products formed by laccase from Methyl Orange in this way are p-N,N'-dimethylamine phenyldiazine and p-hydroxybenzene sulfonic acid (Fig. 2). Although many authors marked these compounds as toxic (WANG et al., 2008), DU et al. (2015) concluded that degradation products (amine and amide derivatives) formed by biological treatment of Methyl Orange in water solution by Aeromonas sp. strain DH-6 have lower phytotoxicity levels tested on Chinese cabbage. Degradation of bis azo dyes is a more complicated process. In the same way, azo bonds are cleaved asymmetrically by laccases (SI et al., 2013; ADNAN et al., 2015; ZHENG et al., 2016) but this reaction requires electrons for reduction (NAM and RENGANATHAN, 2010). Laccases can transfer electron to azo dye because laccases contain four histidine-rich copper binding domains in the catalytic centre (ZHENG et al., 2016). Biodegradation of the bis azo dye Congo Red by high-redox potential laccases from Trametes pubescens (E0 Cu T1 = 738 – 745 ± 5 mV vs. NHE; SHLEEV et al., 2007) led to the formation of naphthalene amine (Fig. 3) but phytotoxic test have shown that the purified laccase can detoxify azo dye Congo Red (SI et al., 2013). This means that Congo Red degradation does not finish in this phase but probably continues forming other non-toxic degradation products. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 96 Legerská et al. Similarly, biodegradation of the bis azo dye Reactive Black 5 by laccase from Trichoderma artroviride F03 was initiated by the cleavage of the bis azo bond and followed by deamination, hydroxylation and sulphonation. The genus Trichoderma is characterized by production of laccases with medium-redox potential (SADHASIVAM et al., 2008). The biodegradation mechanism continued with the aromatic ring fission of naphthalene-1,2,8-triol, where its oxygenated ring at C1 and C2 position was cleaved to 2-(2-carboxy-ethyl)-6-hydroxy-benzoic acid via 8-hydroxy-[1,2]-naphthoquinone. 2-(2-carboxyethyl)-6-hydroxy-benzoic acid can be degraded via two possible pathways (i) it undergoes decarboxylation and methylation to form 2,4-ditertbutylphenol (detected at 7th day) and (ii) it is transformed to benzoic acid by decarboxylation mechanism (ADNAN et al., 2015). Moreover, ADNAN et al. (2015) found out that laccase from T. artroviride D03 did not generate toxic aromatic amines. Similarly, ZHENG et al. (2016) found that Acid Black 172 biodegradation by laccase does not produce toxic amines. H N H N H N H N NH2 NH2 SO3Na SO3Na Asymetric cleavage Decolorization H NH2N H NNH2 O NH2 NH2 SO3Na SO3Na H2NH2N H N NH2 SO3Na Desulfonation Deamination Desulfonation Deamination Oxidative cleavage NH2 HN N Deamination Naphtalene amine Biphenyl Napthalene diazonium Fig. 3. Degradation mechanism of Congo Red by purified laccases from Trametes pubescens (SI et al., 2013). Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 97 3.2 Mechanisms of indigo dye degradation Indigo dyes are organic compounds with a characteristic blue colour obtained from plant material such as Indigo feratinctoria L., Indigofera suffructicosa Mill., Isatis tinctoria L. and Polygonum tinctorium. At present these dyes are produced by chemical synthesis via Baeyer-Drewson reaction. Indigo dyes are non-soluble in water, alcohols and ether because these dyes contain intra- and inter-molecular hydrogen bonds. For the highest solubility of Indigo dyes in water it is necessary to solubilize the dye in water with a base such as sodium hydroxide. Indigo and its derivatives such as Indigo Carmine play a key role in various sectors such as the food, textile or cosmetic industries and medicine because of their colour but they are highly toxic and contact with skin or eyes may cause irritation. The oxidation of Indigo dyes by laccases is observed as the sequential taking of four electrons from the molecule of Indigo. The first step in Indigo and Indigo derivatives degradation is electrochemical oxidation to dehydroindigo (BEGGIATO et al., 1993) followed by an attack of nucleophile (e.g. water) which lead to the incorporation of O-atoms into the degradation products (CAMPOS et al., 2001). The proposed pathway of Indigo degradation by laccase from Trametes hirsuta is shown in Fig. 4-A (CAMPOS et al., 2001). Laccases are able to degrade Indigo via the formation of isatine (indol-2,3-dion). Next isatine is degraded to anthranilic acid (2-aminobenzoic acid) spontaneously via decarboxylation of isatic acid, which is intermediate formed hydrolytically after isatine degradation. N N O H H O N + N O H O N O O H 2 NH2 OH O Isatin Anthranilic acid N N O H H O NaO3S SO3Na NH2 OH O Anthranilic acid N H O SO3Na O Isatin-5-sulfonic acid A B Desulfonation Fig. 4. Degradation mechanism of Indigo (A) and Indigo Carmine (B) by laccase from Trametes hirsuta (CAMPOS et al., 2001; SINGH et al., 2007). Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 98 Legerská et al. YOUNES and SAYADI (2013) found that enzymatic degradation of Indigo Carmine by laccase from the fungus Scytalidium thermophillum reached 98 % decolorization after 24 hours. Toxicity level of degradation products was lower toward bacteria Escherichia coli and Bacillus megaterium than Indigo Carmine itself. The bacterial strain γ-proteobacterium JB and the white-rot fungus T. hirsuta were able to effectively degrade Indigo Carmine to anthranilic acid and isatin-5-sulfonic acid (SINGH et al., 2007), but higher amount of anthranilic acid was measured in the reaction system catalyzed by fungal laccase (98.3 %) than bacterial laccase (56.4 %) (Fig. 4-B). Additionally, laccase from T. hirsuta had the higher rate of reaction. These results were confirmed by CAMPOS et al. (2001) which found that degradation of Indigo is faster in the presence of laccase from the fungal strain T. hirsuta than laccase from the bacterial strain Sclerotium rolfsii, but the same degradation products (anthranilic acid and isatin) were created after biodegradation by both laccases. This difference in the reaction rate was caused by the different redox potential of selected laccases because fungal laccases have higher E0 Cu T1 potential than bacterial laccases (MATE and ALCALDE, 2015). 3.3 Mechanisms of triphenylmethane dye degradation Triphenylmethane (TPM) dyes belong to the group of older dyes with intense colour to be used in the textile, paper, food, cosmetic and leather industries and medicine. The basic structure of these dyes is triphenylmethane. TPM dyes are known as resistant to enzymatic decolorization and the process requires more time for dye degradation (FOROOTANFAR et al., 2012). Although SRIVASTAVA et al. (2004) did not confirm mutagenicity of TPM dyes such as Basic Green 4 and Acid Violet 17 (SRIVASTAVA et al., 2004), Malachite Green (MG) is a typical TPM dye used in farmed fish for controlling of protozoan and fungal infection. Degradation of MG by laccase as well as toxicity of MG and its degradation products has been extensively studied by many authors (KUMAR et al., 2012; ZHUO et al., 2015; YANG et al., 2015). Compared with phytotoxicity level of MG expressed by inhibition of seed germination, phytotoxicity levels of its degradation products were decreased. Similarly, decrease toxicity of TPM dyes after laccase treatment was observed in other studies (KALYANI et al., 2008; SATHISHKUMAR et al., 2013; YANG et al., 2015). Laccases can oxidize the methyl carbon attached in TPM dye structure, giving stable products which are affected by p-substituted phenyl. BIBI et al. (2011) suggested that N-demethylation was the key factor of TPM degradation. CASAS et al. (2009) found that laccases are able to degrade TPM dyes, but non-substituted TPM dyes were not completely degraded by laccases. Laccase from Cyathus bulleri is able to degrade Basic Green 4 via demethylation. YANG et al. (2015) proposed two parallel degradation pathways of MG by laccase of Cerrena sp. (Fig. 5). The first pathway starts with demethylation of MG followed by degradation or polymerization of MG for chromophore destruction. In the second pathway, MG is firstly hydroxylated to its carbinol form (FISHER et al., 2011) which is quickly broken down. The presence of both pathways contributes to fast and efficient degradation of MG by laccases. The pathway is probably depended on laccase type and reaction conditions. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 99 KUMAR et al. (2012) confirmed the formation of four different non-identified degradation products of MG after treatment by laccase from Pleurotus ostreatus. On the other hand, after degradation of Brilliant Green 1 closing related to MG by laccase from Trametes versicolor (E0 Cu T1 = 785 mV vs. NHE; REINHAMMAR et al., 1972), the formation of only two compounds (benzoic acid and diethylamine) were observed (CASAS et al., 2009). NN CH3 CH3 H3C H3C NN CH3 H H3C H3C NN CH3 CH3 H3C H3C I II HydroxylationDemethylation OH Oxidation Demethylation Demethylation N H3C CH3 O N H H H Demethylation Demethylation Demethylation NN H H H H Tetradesmethyl MG (Amino phenyl)-phenyl-methanoneN,N-dimetylanilin Fig. 5. Pathways (I, II) for Malachite Green (MG) degradation by laccase of Cerrena sp. (YANG et al., 2015). 3.4 Mechanisms of anthraquinone dye degradation Anthraquinone dyes are the second most important class of textile dyes (BAUGHMAN and WEBER, 1994). They provide a wide range of colours including violet, blue and green and show excellent long-term colour stability (MENG et al., 2003). The oldest dye from this group is Alizarin which was extracted from the root of durable plant Rubia tinctorum for the first time. The π-conjugated electron system is delocalized in the core and in the substituents. The important anthraquinone dyes Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 100 Legerská et al. commonly used in textile production process are Acid Blue 129 and Remazol Brilliant Blue R (RBBR). These dyes were used as reactive dyes for polymeric dye production and represent the class of often toxic and recalcitrant pollutants. There are several experimental works (YANG et al., 2009; ZENG et al., 2012; AFREEN et al., 2016) concerning the study of an anthraquinone dye decolorization by laccases. Laccases have been shown to decolorize anthraquinone dyes more efficiently than other classes of dyes (ZENG et al., 2011). Additionally ZENG et al. (2012) described anthraquinone dyes acting as redox mediators for azo dye decolorization catalyzed by laccases. During the degradation of anthraquinone dyes by laccase, the chromophore of dye can be broken down forming smaller molecules with potential lower toxicity levels. OSMA et al. (2010) observed biodegradation of RBBR by laccase from T. pubescens and suggested the reaction pathway including the identified intermediates and degradation products (Fig. 6-A). During RBBR degradation by laccase was observed reduction, hydroxylation, deamination, and oxidation reactions. In the later work of HADIBARATA et al. (2011) confirmed the effective decomposition of RBBR dye by laccase from Polyporus sp. S133 (Fig. 6-B) (HADIBARATA et al, 2011). The analogous deamination reaction is not observed in RBBR biodegradation by laccase from Polyporus sp. S133 resulting in products which contain an amino group in their structure. O O HN NH2 SO3Na S O O O SO3Na O SO3Na O S O O O O SO3Na O NH2 H2N S O O O SO3Na OH A B O Fig. 6. Pathway for Remazol Brilliant Blue R degradation by the laccase of Trametes pubescens (A) (OSMA et al., 2010) and laccase of Polyporus sp. S133 (HADIBARATA et al, 2011). Although HADIBARATA et al. (2011) found the presence of low-molecular weight products after laccase-catalyzed reaction but toxicity levels of these products were not lower. On the other hand, OSMA et al. (2010) evaluated phytotoxicity of RBBR and its degradation products after laccase treatment using ryegrass as a representative of grasslands species. Degradation products of RBBR were still toxic for ryegrass (germination index – GI of 69 %) but less than original dye (GI of 26 %). Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 101 4. Conclusion It has been demonstrated that laccases are able to degrade synthetic dyes with different chemical structures. The efficiency of biodegradation is primarily affected by type of laccase-producing organism which determines the redox potential of laccases, different substrate specificities and, last but not least, rates of dye biodegradation. While bacterial and selected plant species produce laccases with a low-redox potential, filamentous fungi produce laccases with medium- or high-redox potential. The white- rot fungi group is particularly interesting in this respect, as producer of high-redox potential laccases. The prediction of degradation products formation after laccase treatment of synthetic dyes is a valuable tool to the evaluation of their potential toxicity. The experimental results presented in the literature suggest that degradation products that form in laccase-catalyzed reaction with synthetic dye have lower toxicity than dye itself. Acknowledgement: This work was supported by FPPV-22-2016. References ADNAN, L.A., SATHISHKUMAR, P., YUSOFF, A.R.M., HADIBARATA, T.: Metabolites characterisation of laccase mediated Reactive Black 5 biodegradation by fast growing ascomycete fungus Trichoderma atroviride F03. Int. Biodeterior. Biodegradation, 104, 2015, 274-282. AFREEN, S., ANWER, R., SINGH, R.K., FATMA, T.: Extracellular laccase production and its optimization from Arthrospira maxima catalyzed decolorization of synthetic dyes. Saudi J. Biol. Sci., 2016, (in press). AITKEN, D.M., MASSEY, I.J., CHEN, T., HECK, P.E.: Characterization of reaction products from the enzyme catalyzed oxidation of phenolic pollutants. Water Res., 28, 1994, 1879-1889. AMIN, K.A., ABDEL, H.H., ABD, E.A.H.: Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem. Toxicol., 48, 2010, 2994-2999. AXON, A., MAY, F.E., GAUGHAN, L.E., WILLIAMS, F.M., BLAIN, P.G., WRIGHT, M.C.: Tartrazine and sunset yellow are xenoestrogens in a new screening assay to identify modulators of human oestrogen receptor transcriptional activity. Toxicology, 298, 2012, 40-51. AZMI, W., SANI, R.K., BANERJEE, U.C.: Biodegradation of triphenylmethane dyes. Enzyme Microb. Technol., 22, 1998, 185-191. BALDRIAN, P.: Fungal laccases - occurrence and properties. FEMS Microbiol. Rev., 30, 2006, 215-242. BAUGHMAN, G.L., WEBER, E.J.: Transformation of dyes and related compounds in anoxic sediment: kinetics and products. Environ. Sci. Technol., 28, 1994, 267-276. BIBI, I., BHATTI, H.N., ASGHER, M.: Comparative study of natural and synthetic phenolic compounds as efficient laccase mediators for the transformation of cationic dye. Biochem. Eng. J., 56, 2011, 225-231. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 102 Legerská et al. BOLLAG, J.M., SHUTTLEWORTH, K.L., ANDERSON, D.H.: Laccase mediated detoxification of phenolic compounds. Appl. Environ. Microbiol., 54, 1998, 3086-3091. CAMPOS, R., KANDELBAUER, A., ROBRA, K.H., CAVACO-PAULO, A., GUBITZ, G.M.: Indigo degradation with purified laccases from Trametes hirsuta and Sclerotium rolfsii. J. Biotechnol., 89, 2001, 131-139. CANAS, A.I., CAMARERO, S.: Laccases and their natural mediators: biotechnological tools for sustainable eco-friendly processes. Biotechnol. Adv., 26, 2010, 694-705. CASAS, N., PARELLA, T., VINCENT, T., CAMINAL, G., SARRA, M.: Metabolites from the biodegradation of triphenylmethane dyes by Trametes versicolor or laccase. Chemosphere, 75, 2009, 1344-1349. CHEN, H.: Recent advances in azo dye degrading enzyme research. Curr. Protein Pept. Sci., 7, 2006, 101-111. CHEN, S.H., TING, A.S.Y.: Biodecolorization and biodegradation potential of recalcitrant triphenylmethane dyes by Coriolopsis sp. isolated from compost. J. Environ. Manage., 150, 2015, 274-280. CHHABRA, M., MISHRA, S., SREEKRISHNAN, T.: Laccase/mediator assisted degradation of triarylmethane dyes in a continuous membrane reactor. J. Biotechnol., 10, 2009, 69-78. CHIVUKULA, M., RENGANATHAN, V.: Phenolic azo dye oxidation by laccase from Pyricularia oryzae. Appl. Environ. Microbiol., 34, 1995, 4374-4377. CHMELOVÁ, D., ONDREJOVIČ, M.: Effect of metal ions on triphenylmethane dye decolorization by laccase from Trametes versicolor. Nova Biotechnol. Chim., 14, 2015, 191-200. DAFALE, N., RAO, N.N., MESHRAM, S.U., WATE, S.R.: Decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium- biostimulation and halo tolerance. Bioresour. Technol., 99, 2008, 2552-2558. DAI, J., WANG, H., CHI, H., WANG, Y., ZHAO, J.: Immobilization of laccase from Pleurotus ostreatus on magnetic separable SiO2 support and excellent activity toward azo dye decolorization. J. Environ. Chem. Engineering., 4, 2016, 2585- 2591. DA SILVA, M.R., DE SA, L.R.V., RUSSO, C., SCIO, E., FERREIRA-LEITAO, V.S.: The use of HRP in decolorization of reactive dyes and toxicological evaluation of their products. Enzyme Res., 2010, 2010, 703824-703831. DAVIES, G.J., DUCROS, V.: Laccase. Part 6. Copper multicopper enzymes. In: Handbook of metalloproteins. John Wiley & Son, New York, 2006, DOI: 10.1002/0470028637.met199. DU, L.N., LI, G., ZHAO, Y.H., XU, H.K., WANG, Y., ZHOU, Y., WANG, L.: Efficient metabolism of the azo dye methyl orange by Aeromonas sp. strain DH-6: characteristics and partial mechanism. Int. Biodeterior. Biodegradation, 105, 2015, 66-72. DURAN, N., ROSA, M.A., D'ANNIBALE, A., GIANFREDA, L.: Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme Microb. Technol., 31, 2002, 907-931. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 103 DURÃO, P., BENTO, I., FERNANDES, A.T., MELO, E.P., LINDLEY, P.F., MARTINS, L.O.: Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies. J. Biol. Inorg. Chem., 11, 2006, 514-526. FABBRINI, M., GALLI, C., GENTILI, P.: Comparing the catalytic efficiency of some mediators of laccase. J. Mol. Cat. B Enz., 16, 2002, 231-240. FISCHER, A.R., WERNER, P., GOSS, K.U.: Photodegradation of malachite green and malachite green carbinol under irradiation with different wavelength ranges. Chemosphere, 2011, 82, 210-214. FORGACS, E., CSERHÁTI, T., OROS, G.: Removal of synthetic dyes from wastewaters: a review. Environ. Int., 30, 2004, 953-971. FOROOTANFAR, H., MOEZZI, A., AGHAIE-KHOZANI, M., MAHMOUDJANLOU, Y., AMERI, A., NIKNEJAD, F., FARAMARZI, M.A.: Synthetic dye decolorization by three sources of fungal laccase. Iran. J. Environ. Health Sci. Eng., 9, 2012, 1-10. GIARDINA, P., FARACO, V., PEZZELLA, C., PISCITELLI, A., VANHULLE, S., SANNIA, G.: Laccases: a never-ending story. Cell. Mol. Life Sci., 67, 2010, 369- 385. GOPINATHAN, R., KANHERE, J., BANERJEE, J.: Effect of malachite green toxicity on non target soil organisms. Chemosphere, 120, 2015, 637-644. HADIBARATA, T., YUSOFF, A.R.M., AYU, R.: Decolorization and metabolism of anthraquinone-type dye by laccase of white-rot fungi Polyporus sp. S133. Water Soil Air Poll., 223, 2011, 933-941. JOHNOSON, D.L., THOMSON, J.L., BRINKMANN, S.M., SCHULLER, K.A., MARTIN, L.L.: Electrochemical characterization of purified Rhus vernicifera laccase: voltammetric evidence for a sequential four-electron transfer. Biochemistry, 2, 2003, 10229-10237. KAGALKAR, A.N., JAGTAP, U.B., JADHAV, J.P., GOVINDWAR, S.P., BAPAT, S.A.: Studies on phytoremediation potentiality of Typhonium flagelliforme for the degradation of Brilliant Blue R. Planta, 232, 2010, 271-285. KALYANI, D.C., PATIL, P.S., JADHAV, J.P., GOVINDWAR, S.P.: Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresour. Technol., 99, 2008, 4635-4641. KLONOWSKA, A., GAUDIN, C., FOURNEL, A., ASSO, M., LE PETIT, J., GIORGI, M., TRON, T.: Characterization of a low redox potential laccase from the basidiomycete C30. Eur. J. Biochem., 269, 2002, 6119-6125. KRUUS, K., KIISKINEN, L.L., SALOHEIMO, M., HAKLINEN, N., ROUVINEN, J., PAANANEN, A., LINDER, M., VIIKARI, L.: A novel laccase from the ascomycete Melanocarpus albomyces. Appl. Microbiol. Biotechnol., 59, 2002, 198-204. KUMAR, V.V., SATHYASELVABALA, V., PREKUMAR, M.P., SIVANESAN, S.: Biochemical characterization of three phase partitioned laccase and its application in decolorization and degradation of synthetic dyes. J. Mol. Cat. B Enz., 74, 2012, 63-72. LI, H., WEBB, S.P., IVANIC, J., JENSEN, J.H.: Determinants of the relative reduction potentials of type-1 copper sites in proteins. J. Amer. Chem. Soc., 126, 2004, 8010-8019. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 104 Legerská et al. MATE, D.M., ALCALDE, M.: Laccase engineering: from rational design to directed evolution. Biotechnol. Adv., 33, 2015, 25-40. MENG, Q., YAN, W., YU, M., HUANG, D.: A study of third-order nonlinear optical properties for anthraquinone derivatives. Dyes Pigm., 56, 2003, 145-149. MOREIRA, S., MILAGRES, A.M.F., MUSSATTO, S.I.: Reactive dyes and textile effluent decolorization by a mediator system of salt-tolerant laccase from Peniophora cinerea. Sep. Purif. Technol., 135, 2014, 183-189. MOT, A.C., SILAGHI-DUMITRESCU, R.: Laccases: complex architectures for one- electron oxidations. Biochemistry (Mosc.), 77, 2012, 1395-1407. NAM, S., RENGANATHAN, V.: Non-enzymatic reduction of azo dyes by NADH. Chemosphere, 40, 2000, 351-357. NATH, P.P., SARKAR, K., MONDAL, M., PAUL, G.: Metanil Yellow impairs the estrous cycle physiology and ovarian folliculogenesis in female rats. Environ. Toxicol., 2015, DOI: 10.1002/tox.22205. OSMA, J.F., TOCA HERRERA, J.L., RODRÍGUEZ COUTO, S.: Transformation pathway of Remazol Brilliant Blue R by immobilised laccase. Bioresour. Technol., 101, 2010, 8509-8514. PANDEY, A., SINGH, P., IYENGAR, L.: Bacterial decolorization and degradation of azo dyes. Int. Biodeterior. Biodegradation, 59, 2007, 73-84. PEREIRA, L., COELHO, A.V., VIEGAS, C.A., CORREIA DOS SANTOS, M.M., ROBALO, M.P., MARTINS, L.O.: Enzymatic biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase. J. Biotechnol., 139, 2009, 68-77. PLATZEK, T., LANG, C., GROHMANN, G., GI, U.S., BALTES, W.: Formation of a carcinogenic aromatic amine from an azo dye by human skin bacteria in vitro. Hum. Exp. Toxicol., 18, 1999, 552-559. POLAK, J., JAROSZ-WILKOLAZKA, A.: Fungal laccases as green catalysts for dye synthesis. Process Biochem., 47, 2012, 1295-1307. RAJAGURU, P., FAIRBAIRN, L.J., ASHBY, J., WILLINGTON, M.A., TURNER, S., WOOLFORD, L.A., CHINNASAMY, N., RAFFERTY, J.A.: Genotoxicity studies on the azo dye Direct Red 2 using the in vivo mouse bone marrow micronucleus test. Mutat. Res., 444, 1999, 175-180. REINHAMMAR, B.R.M.: Oxidation-reduction potentials of the electron acceptors in laccases and stellacyanin. BBA-Bioenergetics, 275, 1972, 245-259. ROBINSON, T., MCMULLAN, G., MARCHANT, R., NIGAM, P.: Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour. Technol., 77, 2001, 247-255. RODRÍGUEZ COUTO, S., TOCA HERRERA, J.L.: Industrial and biotechnological applications of laccases: a review. Biotechnol. Adv., 24, 2006, 500-513. SADHASIVAM, S., SAVITHA, S., SWAMINATHAN, K., LIN, F.H.: Production, purification and characterization of mid-redox potential laccase from a newly isolated Trichoderma harzianum WL1. Process Biochem., 2008, 43, 736-742. SATHISHKUMAR, P., BALAN, K., PALVANNAN, T., KAMALA-KANNAN, S., OH, B.T., RODRÍGUEZ-COUTO, S.: Efficiency of Pleurotus florida laccase on decolorization and detoxification of the reactive dye Remazol Brilliant Blue R (RBBR) under optimized conditions. Clean (Weinh), 41, 2013, 665-672. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC Nova Biotechnologica et Chimica 15-1 (2016) 105 SCHNEIDER, P., CASPERSEN, M.B., MONDORF, K., HALKIER, T., SKOV, L.K., ØSTERGAAT, P.R., BROWN, K.M., BROWN, S.H., XU, F.: Characterization of a Coprinus cinereus laccase. Enzyme Microb. Technol., 25, 1999, 502-508. SHARMA, K.K., SHRIVASTAVA, B., SASTRY, V.R.B., SEHGAL, N., KUHAD, R.CH.:Middle redox potential laccase from Ganoderma sp.: its application in improvement of feed for monogastric animals. Sci. Rep., 1299, 2013, 1-9. SHLEEV, S., JAROSZ-WILKOLAZKA, A., KHALUNINA, A., MOZOROVA, O., YAROPOLOV, A., RUZGAS, T., GORTON, L.: Direct electron transfer reaction of laccase from different origins on carbon electrodes. Bioelectrochem., 65, 2005, 115-125. SHLEEV, S., NIKITINA, O., CHRISTENSON, A., REIMANN, C.T., YAROPOLOV, A.I., RUZGAS, T., GORTON, L.: Characterization of two new multiforms of Trametes pubescens laccase. Bioorg. Chem., 2007, 35, 35-49. SHLEEV, S.V., MOZOROVA, O.V., NIKITA, O.V., GORSHINA, E.S., RUSINOVA, T.V., SEREZHENKOV, V.A., BURBAEV, D.S., GAZARYAN, I.G., YAROPOLOV, A.I.: Comparison of physico-chemical characteristics of four laccases from different basidiomycetes. Biochimie, 86, 2004, 693-703. SI, J., PENG, F., CUI, B.: Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens. Bioresour. Technol., 128, 2013, 49-57. SINGH, G., CAPALASH, N., GOEL, R., SHARMA, P.: A pH-stable laccase from alkali-tolerant γ-proteobacterium JB: purification, characterization and indigo carmine degradation. Enzyme Microb. Technol., 41, 2007, 794-799. SRIVASTAVA, S., SINHA, R., ROY, D.: Toxicological effects of malachite green. Aquat. Toxicol., 66, 2004, 319-329. SUZUKI, T., TIMOFEI, S., KURUNCZI, L., DIETZE, U., SCHŰŰRMANN, G.: Correlation of aerobic biodegradability of sulfonated azo dyes with the chemical structure. Chemosphere, 45, 2001, 1-19. TAUBER, M.M., GUEBITZ, G.M., REHOREK, A.: Degradation of azo dyes by oxidative processes - laccase and ultrasound treatment. Bioresour. Technol., 99, 2008, 4213-4220. TELKE, A.A., GHODAKE, G.S., KAYANI, D.C., DHANVE, R.S., GOVINDWAR, S.P.: Biochemical characteristics of a textile dye degrading extracellular laccase from a Bacillus sp. ADR. Bioresour. Technol., 102, 2011, 1752-1756. TELKE, A.A., KADAM, A.A., JAGTAP, S.S., JADHAV, J.P., GOVINDWAR, S.P.: Biochemical characterization and potential for textile dye degradation of blue laccase from Aspergillus ochraceus NCIM-1146. Biotechnol. Bioprocess Eng., 15, 2010, 696-703. TELKE, A.A., KALYANI, D.C., DAWKAR, V.V., GOVINDWAR, S.P.: Influence of organic and inorganic compounds on oxidoreductive decolorization of sulfonated azo dye C.I. Reactive Orange 16. J. Hazard. Mater., 172, 2009, 298-309. WAN, H., WENG, S., LIANG, S., LU, Q., HE, J.: Evaluation of the developmental toxicity of leucomalachite green administered orally to rats. Food Chem. Toxicol., 49, 2011, 3031-3037. WANG, X., CHENG, X., SUN, D., QI, H.: Biodecolorization and partial mineralization of Reactive Black 5 by a strain of Rhodopseudomonas palustris. J. Environ. Sci., 20, 2008, 1218-1225. Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC 106 Legerská et al. YANG, J., YANG, X., LIN, Y., NG, T.B., LIN, J., YE, X.: Laccase-catalyzed decolorization of malachite green: performance optimization and degradation mechanism. Plos One, 28, 2015, 1-14. YANG, X.Q., ZHAO, X.X., LIU, C.Y., ZHENG, Y., QIAN, S.J.: Decolorization of azo, triphenylmethane and anthraquinone dyes by a newly isolated Trametes sp. SQ01 and its laccase. Process Biochem., 44, 2009, 1185-1189. YOUNES, S.B., SAYADI, S.: Detoxification of Indigo carmine using a combined treatment via a novel trimeric thermostable laccase and microbial consortium. J. Mol. Cat. B: Enz., 87, 2013, 62-68. ZENG, X., CAI, Y., LIAO, X., ZENG, X., LUO, S., ZHANG, D.: Anthraquinone dye assisted the decolorization of azo dyes by a novel Trametes trogii laccase. Process Biochem., 47, 2012, 160-163. ZENG, X., CAI, Y., LIAO, X., ZENG, X., LI, W., ZHANG, D.: Decolorization of synthetic dyes by crude laccase from a newly isolated Trametes trogii strain cultivated on solid agro-industrial residue. J. Hazard. Mater., 187, 2011, 517-525. ZHENG, F., CUI, B.K., WU, X.J., MENG, G., LIU, H.X., SI, J.: Immobilization of laccase onto chitosan beads to enhance its capacity to degrade synthetic dyes. Int. Biodeterior. Biodegradation, 110, 2016, 69-78. ZHOU, X., XIANG, X.: Effect of different plants on azo-dye wastewater bio- decolorization. Procedia Environ. Sci., 18, 2013, 540-546. ZHUO, R., HE, F., ZHANG, X., YANG, Y.: Characterization of a yeast recombinant laccase rLAC-EN3-1 and its application in decolorizing synthetic dye with the coexistence of metal ions and organic solvents. Biochem. Eng. J., 93, 2015, 63-72. ZIMBARDI, A.L.R.L., CAMARGO, R.F., CARLI, S., NETO, S.A., MELEIRO, L.P., ROSA, J.C., DE ANDRADE, A.R., JORGE, J.A., FURRIEL, R.P.M.: A high redox potential laccase from Pycnoporus sanguineus RP15: potential application for dye decolorization. Int. J. Mol. Sci., 17, 2016, 1-42. Received 19 June 2016 Accepted 15 July 2016 Bereitgestellt von Slovenská poľnohospodárska knižnica | Heruntergeladen 17.01.20 14:37 UTC