Barbet_53-58.indd INTRODUCTION Despite extensive investigation over the last 100 years, many vector-borne infectious diseases re- main resistant to human solutions. Some of the most intransigent of these are infections that induce a ‘carrier’ state, where the animal remains infected, is still able to transmit organisms to the vector, but may show disease symptoms intermittently or not at all. Indeed, it may be difficult to detect the infection by classical means. Some examples are those infec- tions caused by African trypanosomes, Babesia par- asites, Anaplasma or Borrelia bacteria (Allred & Al- Khedery 2004; Horn 2004; Palmer, Futse et al. 2006; Bankhead & Chaconas 2007). In many cases, it is now evident through the use of sensitive molecular diagnostic methods that a typical infection course consists of numerous relapsing peaks of organisms separated by time periods when they are not de- tected (Burgdorfer & Mavros 1970; Kieser, Eriks et al. 1990; Calder, Reddy et al. 1996; Zheng, Sentsui et al. 1997; Granquist, Stuen et al. 2008). This has been known for many years with trypanosome in- fections but only more recently for some of the other infectious agents. Nevertheless, some molecular characteristics of these infections resemble those of trypanosomes, but with their own particular special features. I consider here our current understanding of some of these molecular characteristics. DISCUSSION Taking African trypanosomes as the original, most- studied paradigm, we know that the Variable Surface Glycoprotein (VSG) that covers the cytoplasmic 53 Onderstepoort Journal of Veterinary Research, 76:53–58 (2009) Persistence mechanisms in tick-borne diseases A.F. BARBET Department of Infectious Diseases & Pathology, College of Veterinary Medicine University of Florida, Gainesville, Florida, USA ABSTRACT BARBET, A.F. 2009. Persistence mechanisms in tick-borne diseases. Onderstepoort Journal of Vet- erinary Research, 76:53–58 The use of new, highly sensitive diagnostic methods has revealed persistent infections to be a com- mon feature of different tick-borne diseases, such as babesiosis, anaplasmosis and heartwater. Antigenic variation can contribute to disease persistence through the continual elaboration of new surface structures, and we know in several instances how this is achieved. Known or suspected mechanisms of persistence in babesial parasites include cytoadhesion and rapid variation of the ad- hesive ligand in Babesia bovis and genetic diversity in several merozoite stage proteins of different Babesia spp. In Anaplasma, extensive variation in the pfam01617 gene family accompanies cycling of organism levels in chronic infection. One result from the pioneering research at Onderstepoort is the definition of a related polymorphic gene family that is likely involved in immunity against heartwa- ter disease. We are beginning to understand the sizes of the antigenic repertoires and full definition is close, with the possibility of applying simultaneous high-throughput sequencing to the order of 1 000 small genomes. We also, for the first time, can consider modifying these genomes and looking at ef- fects on persistence and virulence. However, important biological questions remain unanswered; for example, why we are seeing a new emerging Anaplasma infection of humans and is infection of endo- thelial cells by Anaplasma significant to persistence in vivo. 54 Persistence mechanisms in tick-borne diseases membrane is responsible for the ensuing battle with the host immune system and we have extensive in- formation about its structure, variation and mecha- nisms of expression (Barry & McCulloch 2001; Van- hamme, Pays et al. 2001; Borst 2002; McCulloch 2004; Pays, Vanhamme et al. 2004). Despite data showing large differences in VSG structure as the trypanosome undergoes antigenic variation during infection, we know that VSG expression is not to- tally random and VSG repertoires are not entirely distinct in different places (Hutchinson, Picozzi et al. 2007). In fact, there are examples of shared vari- ants between VSGs in East and West Africa and South America (Vervoort, Barbet et al. 1981; Mag- nus, Vervoort et al. 1982). Nevertheless, the reper- toires are large (with > 1 500 genes in each trypano- some encoding VSGs) and largely divergent in different places and at different times (Barry, Crowe et al. 1983; Barry, Marcello et al. 2005; Berriman, Ghedin et al. 2005; Hutchinson, Picozzi et al. 2007). Moreover, the use of different segments of these >1 500 genes to create novel trypanosome identi- ties through recombination extends the repertoire size enormously (Roth, Bringaud et al. 1989; Kam- per & Barbet 1992; Barbet & Kamper 1993; Marcello & Barry 2007) . This concept of antigenic repertoire size is useful in analyzing the epidemiology of many relapsing infectious diseases and the possibilities for control. So, what do we know about tick-borne diseases im- portant to animal production? Babesia bovis ex- presses a novel surface antigen, known as the Vari- ant Erythrocyte Surface Antigen (VESA) 1, on the surface of infected erythrocytes (O’Connor, Lane et al. 1997). It is thought to mediate binding of parasit- ized erythrocytes to endothelial cells, thereby caus- ing parasite sequestration in small capillaries (O’Con- nor, Long et al. 1999). This helps the parasite to avoid passage through the spleen and consequent removal. Sequestration in the microvasculature is an important feature of babesiosis and is often found in the dangerous cerebral form of the disease. VESA1 is strongly recognized during bovine infection, with variant-specific antibodies detected shortly after the respective parasitemic peaks containing the homol- ogous organisms. In a recent study (Al-Khedery & Allred 2006), a genomic expression site for VESA1 was described which had acquired at least three dif- ferent short-coding DNA segments from elsewhere in the genome, suggesting an analogous mecha- nism to amplify the VESA1 repertoire in Babesia parasites to that in trypanosomes. The VESA1 gene repertoire size in a single Babesia organism was estimated at > 350 (for one of the two VESA1 subu- nits) (Al-Khedery & Allred 2006), although genome sequence data put the repertoire size at closer to 150 copies (Brayton, Lau et al. 2007), without con- sidering the effects of repertoire amplification by re- combination. Therefore, in both trypanosomes and Babesia, which are protozoans with genome sizes of 35 Mb and 8.2 Mb, respectively, there are large, amplifiable repertoires of variant surface antigens. One might expect the situation to be less complex in Anaplasma because of the smaller genome size of prokaryotes (1.2 Mb for Anaplasma marginale). In fact, this appears to be the case, with two variant outer membrane proteins (OMPs) identified, MSP2 and MSP3 (Barbet, Lundgren et al. 2000; Brayton, Meeus et al. 2003; Meeus, Brayton et al. 2003). Mes senger RNA encoding these molecules is tran- scribed from single genomic expression sites to which approximately seven MSP2 pseudogenes and seven MSP3 pseudogenes contribute sequence by recombination (Brayton, Knowles et al. 2001). As with trypanosomes and Babesia, the variant reper- toire is amplifiable in A. marginale, because of the use of different pseudogenes and pseudogene seg- ments to create mosaic expression site sequences. Although this extends the repertoire of variant MSP2 and MSP3 enormously, the ultimate size is limited by the relatively small pool of donor pseudogenes. An important unknown question is how much this pool of donor sequences varies with time and loca- tion. We do know that many pseudogenes are shared by strains from diverse locations and that epidemic spread of the organism appears associ- ated with the introduction of organisms carrying dif- ferent MSP2 pseudogenes (Rodriguez, Palmer et al. 2005). Anaplasma phagocytophilum is a related species to A. marginale which, although known for > 200 years as a pathogen of ruminants (cases in more than 300 000 Norwegian lambs annually) (Stuen 2007), is now causing severe infections in dogs and hu- mans (Dumler, Choi et al. 2005; Lester, Breitschwerdt et al. 2005; Poitout, Shinozaki et al. 2005; Dumler, Barat et al. 2007; Jensen, Simon et al. 2007; Beall, Chandrashekar et al. 2008; Kohn, Galke et al. 2008). Interestingly, the A. phagocytophilum genome con- tains > 100 genes and pseudogenes homologous to MSP2 of A. marginale and likewise a single expres- sion site to which they donate variant sequences (Barbet, Meeus et al. 2003; Wang, Rikihisa et al. 2004; Lin & Rikihisa 2005; Dunning Hotopp, Lin et al. 2006). As with A. marginale, the A. phagocyto- philum ortholog (MSP2/P44) is immunoprotective against homologous variants (Palmer, Oberle et al. 55 A.F. BARBET 1988; Wang, Kikuchi et al. 2006). Although the po- tential variant repertoire appears larger than in A. marginale, it is known that many similar variants are expressed in the multiple different animal species infected by A. phagocytophilum in the USA, possi- bly indicating a more recent introduction to the con- tinent and less evolutionary diversification of the donor gene repertoire than in European strains (Barbet, Lundgren et al. 2006). Of great significance to Africa is the tick-borne dis- ease of heartwater and, here too, we now know much more of the potential variant OMP repertoire. This is, in large part, due to the pioneering research of the Onderstepoort Veterinary Institute which de- veloped culture methods to grow Ehrlichia (formerly Cowdria) ruminantium (Bezuidenhout, Paterson et al. 1985) and then to obtain a complete genome se- quence (Collins, Liebenberg et al. 2005). We are fortunate in this case to have more than one genome sequence, from both an African and a Caribbean strain (Frutos, Viari et al. 2006) of E. ruminantium. Although the ehrlichias express a related group of variable but immunoprotective (Nyika, Mahan et al. 1998; Nyika, Barbet et al. 2002; Yager, Bitsaktsis et al. 2005) OMPs to the MSP2s of the anaplasmas, they do not appear to have a similar system of anti- genic variation that involves extensive recombina- tion into a single genomic expression site. This is not to say that there are no antigenic differences in OMPs between strains and, in fact, breaks in pro- tection against different strains have been a con- tinual problem for development of vaccines against heart water disease (Jongejan, Thielemans et al. 1991; Jongejan, Vogel et al. 1993; Mahan, Allsopp et al. 1999). The MSP2-related OMPs of E. ruminan- tium are encoded by a tandem group of 16 genes (Van Heerden, Collins et al. 2004) one of which appears to encode the major OMP expressed in mammals and a second the major OMP in ticks (Postigo, Taoufik et al. 2008). This leaves a ques- tion concerning the role of the remaining paralogs. In Ehr lichia chaffeensis, which has a similar genom- ic struc ture of MSP2-related paralogs to E. ruminan- tium, sensitive proteomic methods have detected expression of most paralogs despite the dominant expression of certain paralogs in ticks or mammals (Ge & Rikihisa 2007; Seo, Cheng et al. 2008). Anal- ysis of RNA transcripts has suggested that the same may be true for E. ruminantium (Bekker, Postigo et al. 2005). It is also clear that both major and minor expressed paralogs are variant in different strains of E. ruminantium throughout Africa and the Caribbean (Reddy, Sulsona et al. 1996; Barbet, Byrom and Mahan, unpublished). Therefore, all paralogs could be involved in expression of the total antigenic iden- tity of E. ruminantium, as they appear to be in Ana- plasma. In A. marginale, an interesting hypothesis has been proposed that the ability of strains to superinfect (and ultimately to cause epidemic spread and influ- ence cross-protection by vaccines) depends on whether or not a superinfecting strain has a different donor pseudogene (for OMP expression) repertoire (Futse, Brayton et al. 2008). If it does, then the su- perinfecting strain can overcome pre-existing immu- nity developed over a long time period of infection in carrier animals. This is supported by data from the MSP2 pseudogene repertoire and, currently, the in- volvement of the other known variant OMP, MSP3, in this process is not clear. There are opportunities to test this theory in other systems. For example, despite the > 100 genes and pseudogenes poten- tially contributing to MSP2/P44 variants in A. phago- cytophilum, there are two US strains, MRK and HZ, which differ in (presence or absence of) only two pseudogenes (Dunning Hotopp, Lin et al. 2006). One might predict heavy usage of these two pseu- dogenes in a superinfection of MRK-infected (long- term carrier) animals by HZ. If true for other tick- borne diseases, knowledge of the total repertoire of contributing variant genes to OMP diversity becomes of paramount importance to understand disease epi- demiology and for vaccine development. Con ceiv- ably, this might be achieved for the smaller genome organisms using second and third generation ge- nome-sequencing capable of obtaining, e.g. 1 000 genome sequences of diverse E. ruminantium strains (Margulies, Egholm et al. 2005; Holt & Jones 2008). Complementary to the new power of genome se- quencing technology to evaluate variant repertoires in tick-borne diseases is the ability to introduce for- eign DNA and investigate phenotypic effects. This has been achieved with A. phagocytophilum by transposon mutagenesis (Felsheim, Herron et al. 2006), but directed methods of gene knock-out in A. marginale, Babesia or Ehrlichia are still lacking, al- though standard in trypanosomes and other para- sites and bacteria for many years. Potentially, these methods offer the ability to verify definitively the im- portance of OMP variation in persistent infections, as well as to use other approaches towards vaccines through deliberate attenuation. 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