30 Babelyuk Valeriy Ye., Popadynets’ Oleksandr O., Dubkova Galyna I., Zukow Walery, Muszkieta Radosław, Gozhenko Anatoliy I., Popovych Igor L. Entropy of gas-discharge image correlates with the entropies of EEG, immunocytogram and leukocytogram but not HRV. Pedagogy and Psychology of Sport. 2020;6(2):30-39. elSSN 2450-6605. DOI http://dx.doi.org/10.12775/PPS.2020.06.02.003 https://apcz.umk.pl/czasopisma/index.php/PPS/article/view/PPS.2020.06.02.003 https://zenodo.org/record/3755035 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2020; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 01.04.2020. Revised: 16.04.2020. Accepted: 16.04.2020. ENTROPY OF GAS-DISCHARGE IMAGE CORRELATES WITH THE ENTROPIES OF EEG, IMMUNOCYTOGRAM AND LEUKOCYTOGRAM BUT NOT HRV Valeriy Ye. Babelyuk1,2, Oleksandr O. Popadynets’1, Galyna I. Dubkova2, Walery Zukow3, Radosław Muszkieta3, Anatoliy I. Gozhenko1, Igor L. Popovych1,4 1Ukrainian Scientific Research Institute of Medicine for Transport MHU, Odesa, Ukraine 2Clinical Sanatorium „Моldоvа”, Truskavets’, Ukraine san.moldova.tr@ukr.net 3Nicolaus Copernicus University, Torun, Poland w.zukow@wp.pl 4OO Bohomolets’ Institute of Physiology NAS, Kyїv, Ukraine i.popovych@biph.kiev.ua Abstract Background. In implementing the project of verification gas discharge visualization (kirlianography, biophotonics) method, we documented the significant correlation of the gas discharge image parameters with the parameters of electroencephalogram, heart rate variability (HRV), dexterity and spasticity of brush, blood pressure, as well as blood levels of adaptive hormones. As part of a project to investigate the physiological nature of entropy, we have shown that EEG entropy is related to a number of its amplitude-frequency and spectral parameters, as well as to the parameters HRV, blood leukocytogram and immunocytogram and their entropies. The purpose of this study is to analyze the relationships between the entropies of the listed information systems. Material and research methods. The object of observation were 20 volunteers: 10 women and 10 men aged 33-76 years without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism. We registered twice kirlianogram by the method of GDV by the device of “GDV Chamber” (“Biotechprogress”), EEG (“NeuroCom Standard”, KhAI Medica), HRV ("CardioLab+HRV", "KhAI-Medica"), Leukocytogram and Immunocytogram. Than we calculated the entropies of the listed information systems. Results. By stepwise exclusion, 5 variables were included in the canonical GDV root structure, and 6 variables were included in the root EEG structure. Overall, GDI entropy determines the SPD EEG entropy by 33%. The additional inclusion in the dependent set the parameters of HRV, LCG and ICG entropies gives a increase in determination to 48%. HRV entropy was found outside the model. Conclusion. We have documented the relationship http://dx.doi.org/10.12775/PPS.2020.06.02.003 https://apcz.umk.pl/czasopisma/index.php/PPS/article/view/PPS.2020.06.02.003 https://zenodo.org/record/3755035 mailto:san.moldova.tr@ukr.net mailto:w.zukow@wp.pl mailto:i.popovych@biph.kiev.ua 31 between the entropy parameters of electroencephalogram, blood leukocytogram and immunocytogram but not HRV on the one hand, and gas-discharge images on the other. However, the question of the causal nature of this relationship remains open. What is primary: electrical activity of the brain, excretion of cytokines and hormones by immunocytes, or emission of photons and free electrons by acupuncture points (circulation of vital energy)? Key words: gas-discharge visualization; electroencephalogram; HRV; blood leukocytogram and immunocytogram; entropy, relationships. INTRODUCTION In implementing the project of verification gas discharge visualization (kirlianography, biophotonics) method proposed by KG Korotkov [17,18], we documented the significant correlation of the gas discharge image (GDI) parameters with the parameters of electroencephalogram [4,8,9,16,19,20,30,31], HRV [4,8-10,19,20,28,29,31], dexterity and spasticity of brush [4,9,19,20,31], blood pressure [28,29], as well as blood levels of adaptive hormones [1-3,5-7,10,11]. As part of a project to investigate the physiological nature of entropy, we have shown that EEG entropy is related to a number of its amplitude-frequency and spectral parameters, as well as to the parameters HRV, blood leukocytogram and immunocytogram and their entropies [13,22-27,33]. The purpose of this study is to analyze the relationships between the entropies of the listed information systems. MATERIAL AND RESEARCH METHODS The object of observation were 20 volunteers: ten women and ten men aged 33-76 years without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism, characteristic for premorbid (intermediate between health and illness) state. In the morning on an empty stomach we registered kirlianogram by the method of GDV by the device of “GDV Chamber” (“Biotechprogress”, SPb, RF). The first base parameter of GDV is Area of gas discharge image (GDI) in Right, Frontal and Left projections registered both with and without polyethylene filter. The second base parameter is the Shape coefficient (ratio of square of length of external contour of GDI toward his area), which characterizes the measure of serration/fractality of external contour. The third base parameter of GDI is the Entropy [17,18]. The most recent set of parameters was selected for further analysis in this article. Than we recorded EEG (hardware-software complex “NeuroCom Standard”, KhAI Medica) monopolar in 16 loci (Fp1, Fp2, F3, F4, F7, F8, C3, C4, T3, T4, P3, P4, T5, T6, O1, O2) by 10- 20 international system, with the reference electrodes A and Ref on the tassels of ears. Among the options considered the normalized (%) spectral power density (SPD) in the standard frequency bands: β (35÷13 Hz), α (13÷8 Hz), θ (8÷4 Hz) and δ (4÷0,5 Hz) in all loci, according to the instructions of the device. Simultaneosly we recorded electrocardiogram in II lead (hardware-software complex "CardioLab+HRV", "KhAI-Medica") to assess the parameters of HRV. For further analysis 32 (Frequency Domain Methods) were selected normalized (%) spectral power (SP) bands of HRV: high-frequency (HF, range 0,4÷0,15 Hz), low-frequency (LF, range 0,15÷0,04 Hz), very low- frequency (VLF, range 0,04÷0,015 Hz) and ultra low-frequency (ULF, range 0,015÷0,003 Hz) [15]. We calculated for HRV and each locus EEG the Entropy (h) of normalized SPD using formulas [22] based on classic CE Shannon’s formula: hHRV = - [SPHF•log2SPHF+SPLF•log2SPLF+SPVLF•log2SPVLF+SPULF•log2SPULF]/log24; hEEG = - [SPDα•log2SPDα+SPDβ•log2SPDβ+SPDθ•log2SPDθ+SPDδ•log2SPDδ]/log24 In portion of capillary blood we counted up Leukocytogram (LCG) (Eosinophils, Stub and Segmentonucleary Neutrophils, Lymphocytes and Monocytes) and calculated its Entropy (h) using formula: hLCG = - [Lym•log2Lym+Mon•log2Mon+Eos•log2Eos+SNN•log2SNN+StubN•log2StubN]/log25 For phenotyping subpopulations of lymphocytes used the methods of rosette formation with sheep erythrocytes on which adsorbed monoclonal antibodies against receptors CD3, CD4, CD8, CD22 and CD16 from company "Granum" (Kharkiv) with visualization under light microscope with immersion system [21]. Next we calculated also the Entropy of Immunocytogram (ICG) using formula: hICG = - [CD4•log2CD4+CD8•log2CD8+CD22•log2CD22+CD16•log2CD16]/log24 Every day four people were tested. A week later, all the tests were repeated. Results processed using the software package "Statistica 5.5". RESULTS AND DISCUSSION According to the formula: |r|≥{exp[2t/(n-1,5)0,5] - 1}/{exp[2t/(n-1,5)0,5] + 1}, for a sample of 40 observations critical value of correlation coefficient module at p<0,05 (t>2,02) is 0,31, at p<0,01 (t>2,70) is 0,41, at p<0,001 (t>3,55) is 0,52. In the first stage of the analysis a correlation matrix is created (Table 1). 33 Table 1. Correlation matrix for Entropies of gas-discharge image, spectral power density EEG loci, HRV, Leukocytogram and Immunocytogram Entropy Right GDI Right GDI (f) Frontal GDI Frontal GDI (f) Left GDI Left GDI (f) Right GDI 1,00 ,46 ,71 ,42 ,58 ,31 Right GDI (f) ,46 1,00 ,50 ,69 ,49 ,64 Frontal GDI ,71 ,50 1,00 ,58 ,77 ,36 Frontal GDI (f) ,42 ,69 ,58 1,00 ,50 ,64 Left GDI ,58 ,49 ,77 ,50 1,00 ,44 Left GDI (f) ,31 ,64 ,36 ,64 ,44 1,00 Fp2 ,09 ,10 ,18 ,16 ,08 ,06 F4 ,08 -,07 ,16 -,04 ,07 -,14 F8 -,01 -,19 -,02 -,24 -,07 -,46 T4 -,22 -,08 -,06 -,27 -,02 -,16 C4 ,03 -,07 -,06 -,04 -,17 -,19 T6 ,17 -,01 ,03 -,12 -,05 -,08 P4 ,24 ,20 ,19 ,27 ,13 ,02 O2 ,21 ,21 -,02 ,06 ,04 ,11 Fp1 -,00 -,07 -,06 ,11 -,15 ,05 F3 ,19 ,14 ,28 ,19 ,25 -,02 F7 -,03 -,03 ,08 ,10 -,19 -,11 T3 -,22 -,05 -,07 -,08 -,24 -,22 C3 ,01 -,14 -,17 -,11 -,24 -,19 T5 ,10 -,17 -,07 -,10 -,07 -,14 P3 ,20 ,12 ,09 ,20 ,04 ,05 O1 ,16 ,24 ,13 ,31 ,17 ,19 HRV -,10 -,05 -,08 -,24 -,21 -,10 LCG ,30 -,08 ,30 ,18 ,18 ,14 ICG -,25 -,18 -,26 -,21 -,27 -,30 To visualize correlations we should decide concerning factor (argument) and effective (function) parameters. As stated in the previous article, in terms of mathematics it does not matter, while in terms of physiology there is the perennial problem of cause and effect. We have chosen as a factor GDV parameters [10]. As a result of the screening, the most significant relationship was found between the GDI entropy (filtered) in the left projection and the SPD EEG entropy in the right lateral frontal locus (Fig. 1). Unfortunately, intrigue about cross-linking such as the corticospinal pyramid tract has been dispelled by other facts. The inclusion in the multiple regression model of the second, by the power of the link, variable brought about the aesthetic pleasure of the three-dimensional image (Fig. 2), but no more, judging by R. 34 F8H = 2,3630 - ,4318 * ELF Correlation: r = -,4553 3,3 3,4 3,5 3,6 3,7 3,8 3,9 4,0 4,1 ELF 0,3 0,4 0,5 0,6 0,7 0,8 0,9 1,0 F8H 95% confidence Fig. 1. Scatterplot of correlation between the entropy GDI (filtered) on the Left projection (X-axis) and the entropy in F8 locus EEG (Y-axis) Quadratic Surface Z = Distance Weighted Least Squares 0,8 0,6 0,4 0,2 0 F8h=2,22-0,472•ELf+0,081•EFf; R=0,458; R2=0,210; F(2,4)=4,9; p=0,013 Fig. 2. Scatterplot of dependence of entropy in F8 locus EEG (Z-axis) on entropies GDI (filtered) on the Left (X-axis) and Frontal (Y-axis) projections 35 In the next step, the canonical correlation between the entropy indices of the gas-discharge image taken without a filter and with a filter in three projections, on the one hand, and SPD 16 EEG loci, on the other, was analyzed. By stepwise exclusion, 5 variables were included in the canonical GDV root structure, and 6 variables were included in the root EEG structure (Table 2). Judging by the factor loadings, the causal root represents directly, mainly, the entropy of GDI (with filter) in the left projection, while the entropy of GDI (without filter) in the right projection reflects inversely. On the other hand, the EEG root reflects the SPD entropy at five loci inversely and only one directly. Overall, GDI entropy determines the SPD EEG entropy by 33% (Fig. 3). Table 2. Factor structure of canonical correlation between Entropy of GDI (right set) and EEG (left set) Right set R Left GDI (f) ,536 Left GDI ,158 Right GDI -,420 Frontal GDI (f) -,084 Frontal GDI -,055 Left set R F8H -,514 P4H -,478 C3H -,454 F3H -,237 O1H -,141 T4H ,360 hGDV E ntropy E E G -2 -1 0 1 2 -2 -1 0 1 2 R=0,575; R2=0,330; χ2(30)=38; p=0,158; Λ Prime=0,341 Fig. 3. Scatterplot of canonical correlation between Entropy of GDI (X-line) and EEG (Y- line) 36 The additional inclusion in the left set parameters of HRV, LCG and ICG entropies gives a significant increase in the canonical relationship between the roots. This changes the factor structure of the roots. Contrary to expectations, HRV entropy was found outside the model (Table 6 and Figure 4). Table 6. Factor structure of canonical correlation between Entropy of GDI (right set) and EEG,LCG&ICG (left set) Right set R Right GDI ,50 Frontal GDI ,24 Frontal GDI (f) ,24 Left GDI (f) -,33 Right GDI (f) -,28 Left GDI -,03 Left set R LCGH ,477 P4H ,335 C3H ,287 F8H ,229 F3H ,183 O1H ,121 T4H -,440 ICGH -,006 hGDV E ntropy E E G ,IC G ,LC G -2 -1 0 1 2 -2 -1 0 1 2 R=0,699; R2=0,489; χ2(48)=54; p=0,262; Λ Prime=0,181 Fig. 4. Scatterplot of canonical correlation between Entropy of GDI (X-line) and EEG as well as LCG&ICG (Y-line) 37 CONCLUSION We have documented the relationship between the entropy parameters of electroencephalogram, blood leukocytogram and immunocytograms on the one hand, and gas- discharge images on the other. However, the question of the causal nature of this relationship remains open. What is primary: electrical activity of the brain, excretion of cytokines and hormones by immunocytes, or emission of photons and free electrons by acupuncture points [17,18] (circulation of vital energy [32])? ACKNOWLEDGMENT We express sincere gratitude to administration of JSC “Truskavets’kurort” and “Truskavets’ SPA” for help in conducting this investigation. ACCORDANCE TO ETHICS STANDARDS Tests in patients are conducted in accordance with positions of Helsinki Declaration 1975, revised and complemented in 2002, and directive of National Committee on ethics of scientific researches. During realization of tests from all participants the informed consent is got and used all measures for providing of anonymity of participants. For all authors any conflict of interests is absent. REFERENCES 1. Babelyuk VYe. The parameters of gaz discharge visualization (kirlianogram) appropriately associated with some psychophysiological and endocrine parameters of healthy men. Medical Hydrology and Rehabilitation. 2013; 11(1): 21-30. 2. Babelyuk VYe, Dubkova GI, Kikhtan VV, Korolyshyn TA, Popovych IL. The relationship between the parameters of gas discharge visualization and neuro-endocrine regulation [in Ukrainian]. In: Valeology: current status, trends and prospects development. Abstracts XIV International scientific and practical conference (Kharkiv-Drohobych, 14-16 April 2016). Kharkiv. VN Karazin KhNU; 2016: 360-361. 3. Babelyuk VYe, Dubkova GI, Kikhtan VV, Korolyshyn TA, Zukow W, Popovych IL. The parameters of gas discharge visualization and principal neuroendocrine factors of adaptation closely correlated. In: IX International symposium “Actual problems of biophysical medicine” (Kyiv, 12-15 May 2016). Кyiv. OO Bohomolets’ Institute of Physiology; 2016: 9-10. 4. Babelyuk VY, Dubkova HI, Korolyshyn TA, Mysula IR, Popovych DV, Popovych IL, Zukow W. Relationships between caused by Kozyavkin© method changes in parameters of manual function and electroencephalogram, heart rate variability as well as gas discharge visualization in children with spastic form of cerebral palsy. Journal of Education, Health and Sport. 2018; 8(4): 159-194. 5. Babelyuk VYe, Dubkova GI, Korolyshyn TA, Zukow W, Popovych IL. The correlationships between parameters of gas discharge visualization and principal neuroendocrine factors of adaptation. In: Pathophysiology and Pharmacy: ways of integration. Abstracts VII National Congress of Pathophysiologists Ukraine with international participation (5-7 October 2016). Kharkiv; NPhU: 8. 6. Babelyuk VYe, Dubkova GI, Popovych IL. Correlation parameters of electrophotonics (kirlianogram) with neuroendocrine parameters [in Ukrainian]. In: Materials V scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil’, 1-2 November 2012): Achievements of Clinical and Experimental Medicine. 2012; 2(17): 158. 7. Babelyuk VYe, Dubkova GI, Popovych IL. Gas discharge visualization parameters correlate with some psycho-physiological and endocrine parameters of healthy men [in Ukrainian]. In: “Actual 38 problems of biophysical medicine”. Materials VII International symposium (Kyiv, 14-17 May 2014). Кyiv: OO Bohomolets’ Institute of Physiology, 2014: 11-13. 8. Babelyuk VYe, Dubkowa GI, Korolyshyn TA, Holubinka SM, Dobrovol’s’kyi YG, Zukow W, Popovych IL. Operator of Kyokushin Karate via Kates increases synaptic efficacy in the rat Hippocampus, decreases C3-θ-rhythm SPD and HRV Vagal markers, increases virtual Chakras Energy in the healthy humans as well as luminosity of distilled water in vitro. Preliminary communication. Journal of Physical Education and Sport. 2017; 17(1): 383-393. 9. Babelyuk VY, Dubkova HI, Korolyshyn TA, Mysula IR, Popovych DV, Popovych IL, Zukow W. Relationships between caused by Kozyavkin© method changes in parameters of manual function and electroencephalogram, heart rate variability as well as gas discharge visualization in children with spastic form of cerebral palsy. Journal of Education, Health and Sport. 2018; 8(4): 159-194. 10. Babelyuk VE, Gozhenko AI, Dubkova GI, Babelyuk NV, Zukow W, Kovbasnyuk MM, Popovych IL. Causal relationships between the parameters of gas discharge visualization and principal neuroendocrine factors of adaptation. Journal of Physical Education and Sport. 2017; 17(2): 624- 637. 11. Babelyuk VYe, Popovych IL. Some biophysical and hematological correlates testosteronemia levels in healthy men [in Ukrainian]. Zdorovye muzhchiny. Health males. 2013; 2(45): 180-181. 12. Dobrovol’s’kyi YuG, Gozhenko AI, Babelyuk VYe, Popovych IL. Method of water structuredness study with discharge-optical device and demonstration possibility of energy-information influence on it operator [in Ukrainian]. Water: Hygiene and Ecology. 2013; 2(1): 120-135. 13. Gozhenko AI, Popadynets’ OO, Zukow WA, Popovych IL. Differences in parameters of the EEG and HRV in the humans with various levels of the entropy of EEG, HRV, immunocytogram and leukocytogram. In: Collection of Proceedings of the Scientific and Practical Conference: Galician Readings "Contemporary ideas on the pathogenesis of inflammation: local and systemic mechanisms" (Ivano-Frankivs’k, 19-20 September). Ivano-Frankivs’k. IFNMU; 2019: 73-74. 14. Gozhenko AI, Sydoruk NO, Babelyuk VYe, Dubkowa GI, Flyunt VR, Hubyts’kyi VYo, Zukow W, Barylyak LG, Popovych IL. Modulating effects of bioactive water Naftussya from layers Truskavets’ and Pomyarky on some metabolic and biophysic parameters at humans with dysfunction of neuro-endocrine-immune complex. Journal of Education, Health and Sport. 2016; 6(12): 826-842. 15. Heart Rate Variability. Standards of Measurement, Physiological Interpretation, and Clinical Use. Task Force of ESC and NASPE. Circulation. 1996; 93(5): 1043-1065. 16. Kindzer BM, Babelyuk VY, Babelyuk NV, Popovych IL, Dubkova GI, Dobrovolskyi YG, Korsuns’kyi IH, Korolyshyn TA, Litosh S, Kindzer H, Zukow W. The device for electrostimulation "VEB-1" modulates parameters of electroencephalogram and gas discharge visualization. Science and society. Proc of the 11th internat. confer. Acent Grafics Communications and Publishing. Hamilton, Canada; 2019: 159-171. 17. Korotkov KG. Basics GDV Bioelectrography [in Russian]. SPb.: SPbGITMO(TU), 2001. 360 p. 18. Korotkov KG. Principles of Analysis in GDV Bioelectrography [in Russian]. SPb. Renome; 2007: 286 p. 19. Kozyavkina OV, Kozyavkina NV, Hordiyevych МS, Voloshyn ТB, Lysovych VI, Babelyuk VY, Dubkova HI, Korolyshyn TA, Popovych DV, Mysula IR, Zukow W, Popovych IL. Forecasting caused by Kozyavkin© method changes in hand function parameters in children with spastic form of cerebral palsy at their baseline levels as well as EEGs, HRVs and GDVs. Achivements of Clinical and Experimental Medicine. 2018; 4: 17-35. 20. Kozyavkina OV, Kozyavkina NV, Voloshyn ТB, Hordiyevych МS, Lysovych VI, Babelyuk VY, Dubkova HI, Korolyshyn TA, Mysula IR, Popovych DV, Zukow W, Popovych IL. Caused by Kozyavkin© method changes in hand function parameters in children with spastic form of cerebral palsy and their EEGs, HRVs and GDVs accompaniments. Journal of Education, Health and Sport. 2018; 8(10): 11-30. 39 21. Lapovets’ LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. L’viv; 2002: 173 p. 22. Popadynets’ OO, Gozhenko AI, Zukow W, Popovych IL. Relationships between the entropies of EEG, HRV, immunocytogram and leukocytogram. Journal of Education, Health and Sport. 2019; 9(5): 651-666. 23. Popadynets’ OO, Gozhenko AI, Zukow W, Popovych IL. Interpersonal differences between of the entropies of EEG, HRV, immunocytogram and leukocytogram. Journal of Education, Health and Sport. 2019; 9(6): 534-545. 24. Popadynets’ OO, Gozhenko AI, Zukow W, Popovych IL. Peculiarities of spectral parameters of EEG, HRV and routine parameters of immunity in patients with various levels of the entropy of EEG, HRV, immunocytogram and leukocytogram. Journal of Education, Health and Sport. 2019; 9(8): 617-636. 25. Popadynets’ OO, Gozhenko AI, Badiuk NS, Zukow W, Popovych IL. Interpersonal differences between caused by adaptogens changes in the entropies of eeg, hrv, immunocytogram and leukocytogram. In: Rehabilitation Medicine and Health-Resort Institutions Development. Proceedings of the 19th International Applied Research Conference (Kyїv, 11-12 December 2019). Edited by O. Gozhenko, W. Zukow. Toruń, Kyiv. 2019: 51-53. 26. Popadynets’ OO, Gozhenko AI, Badiuk NS, Zukow W, Kovbasnyuk MM, Korolyshyn TA, Popovych IL. Relationships between changes in entropy of the EEG and parameters of the immunity. Pedagogy and Psychology of Sport. 2020; 6(1): 24-40. 27. Popadynets’ O, Gozhenko A, Badyuk N, Popovych I, Skaliy A, Hagner-Derengowska M, Napierata M, Muszkieta R, Sokołowski D, Zukow W, Rybałko L. Interpersonal differences caused by adaptogen changes in enropies of EEG, HRV, immunocytogram, and leukocytogram. Journal of Physical Education and Sport. 2020; 20(Suppl. 2): in print. 28. Popovych IL, Babelyuk VYe, Dubkova GI. Parameters of bioelectrography (kirlianography) is closely correlated with parameters of heart rate variability and blood pressure [in Ukrainian]. In: IX VV Podvysotskyi reading: Bulletin Materials Scientic Conference (Odesa, 27-28 May 2010). Odesa: ОSMU, 2010: 143-144. 29. Popovych IL, Babelyuk VYe, Dubkova GI. Relations between the parameters bioelectrography (kirlianography) and heart rate variability and blood pressure [in Ukrainian]. Medical Hydrology and Rehabilitation. 2010; 8(1): 4-16. 30. Popovych IL, Babelyuk VYe, Korolyshyn TA, Dubkova GI. Immediate influence of Christian prayer on the parameters of electroencephalogram and kirlianogram [in Ukrainian]. In: Materials VI scientific-practical conference "Issues of pathology in conditions of extreme factors action on the body" (Ternopil’, 31 Oktober-1 November 2013): Achievements of Clinical and Experimental Medicine. 2013; 2(19): 275-276. 31. Popovych IL, Babelyuk VY, Dubkova HI, Korolyshyn TA, Zukow W. Relationships between changes in parameters of manual function and electroencephalogram, heart rate variability as well as gas discharge visualization in children with spastic form of cerebral palsy caused by Kozyavkin© method. Experimental and Clinical Physiology and Biochemistry. 2018; 1(81): 39-50. 32. Puchko LG. Multidimensional Medicine. Systen of Self-diagnosis and Self-healing of Human [in Russian]. 10th ed, rev and ext. Мoskva. ANS; 2004: 432 p. 33. Zukow W, Popadynets’ OO, Gozhenko AI, Popovych IL. Interpersonal differences in parameters of the EEG and HRV in the humans with various levels of the entropy of the EEG, HRV, immunocytogram and leukocytogram. Journal of Education, Health and Sport. 2019; 9(7): 448- 466.