# bib

id: cord-256303-bpa571ys
author: Hotez, Peter J.
title: Will COVID-19 become the next neglected tropical disease?
date: 2020-04-10
extension: .txt
mime: text/plain
words: 565
sentence: 20
flesch: 50
cache: cache/cord-256303-bpa571ys.txt
txt: txt/cord-256303-bpa571ys.txt
summary:
  Currently, the major low-and middle-income nations such as Bangladesh, Brazil, Democratic Republic of Congo, India, Indonesia, Mexico, Nigeria, Pakistan, Philippines, and South Africa, as well as Central American and other nations are beginning to report an increase in COVID-19 cases, but the numbers are still relatively small. Based on the levels of illness we have seen to date in the Northern Hemisphere, we are especially worried about the fate of thousands of dedicated doctors, nurses, and other health care providers.


id: cord-306952-cpltrsa7
author: de Souza, Pedro Mansueto Melo
date: 2020-11-04
extension: .txt
mime: text/plain
words: 1245
sentence: 33
flesch: 36
cache: cache/cord-306952-cpltrsa7.txt
txt: txt/cord-306952-cpltrsa7.txt
title:
  Validation of verbal autopsy and nasopharyngeal swab collection for the investigation of deaths at home during the COVID-19 pandemics in Brazil

summary:
  It is a noteworthy fact that there were no confirmed cases of SARS-CoV-2 among home deaths in the last 5 weeks, which may represent both a decrease in the circulation of the Validation of verbal autopsy for the investigation of deaths at home during the COVID-19 virus as well as the reestablishment of the capacity of the health services of providing medical assistance to possible new cases of the disease. To fulfill its mission, SVO-RF performs complete diagnostic autopsies (CDA) for the investigation of deaths at home without medical assistance, as well as deaths without bona fide diagnosis prior to death


id: cord-260693-8mfuwx8l
author: Seelig, Frederik
title: The COVID-19 pandemic should not derail global vector control efforts
date: 2020-08-31
extension: .txt
mime: text/plain
words: 1130
sentence: 47
flesch: 39
cache: cache/cord-260693-8mfuwx8l.txt
txt: txt/cord-260693-8mfuwx8l.txt
summary:
  [7, 8] , with respect to inter-and intrasectoral collaboration, engagement and mobilisation of communities, and scaling up of vector control if required, according to the implementation plan of vector control activities, while adapting activities as necessary to prevent further spread of COVID-19, in particular vector surveillance, which may need to be scaled down [9, 10] . In view of these combined challenges, we reiterate our solidarity with the global partners who are dealing with the COVID-19 pandemic, while strongly urging them to consider these recommendations for the control of VBDs: • Continue the implementation of the WHO's global vector control response 2017-2030 (GVCR) strategy and regional policies for vector control


id: cord-268329-apl6n6jl
author: Antunes, Douglas Eulálio
title: Will cases of leprosy reaction increase with COVID-19 infection?
date: 2020-07-17
extension: .txt
mime: text/plain
words: 1509
sentence: 53
flesch: 36
cache: cache/cord-268329-apl6n6jl.txt
txt: txt/cord-268329-apl6n6jl.txt
summary:
  In both reactions, we warn of the possible effect that COVID-19 infection may have on the number of cases of these immunological events because the presence of infection is an important risk factor for triggering leprosy reactions [8] . These precautions will aid prevention of the spread of COVID-19 infection-and its severe manifestations, including leprosy reactions. Evaluation and Treatment Coronavirus (COVID-19)


id: cord-260412-yjr83ef6
author: Hotez, Peter J.
title: Developing a low-cost and accessible COVID-19 vaccine for global health
date: 2020-07-29
extension: .txt
mime: text/plain
words: 2343
sentence: 92
flesch: 38
cache: cache/cord-260412-yjr83ef6.txt
txt: txt/cord-260412-yjr83ef6.txt
summary:
  For instance, Texas Children's Center for Vaccine Development (CVD) at Baylor College of Medicine, in collaboration with its nonprofit product development partners-Seattle-based PATH and Infectious Disease Research Institute (IDRI)-have been spearheading a coronavirus vaccine program focusing on recombinant subunit protein vaccines produced in a globally available microbial fermentation platform, and optimized to maximize yield following expression and protein purification [7, 8] . Regarding the former, against SARS CoV homologous virus challenge the vaccine formulated on alum exhibits high levels of protective immunity and with evidence of minimal or no immune enhancement [10] .


id: cord-001484-va0teako
author: Ahmed, Sarah A.
title: Rapid Identification of Black Grain Eumycetoma Causative Agents Using Rolling Circle Amplification
date: 2014-12-04
extension: .txt
mime: text/plain
words: 2925
sentence: 134
flesch: 44
cache: cache/cord-001484-va0teako.txt
txt: txt/cord-001484-va0teako.txt
summary:
  Ligation conditions were: 5 min denaturation at 94uC, followed by 7 cycles of 94uC for 30 sec, 63uC for 4 min, and final cooling at 10uC. Prior to RCA amplification reaction and in order to reduce the ligation-independent amplification, ligation products were treated by addition of 10 U exonucleases I and 10 U exonucleases III (New England Biolabs, Hitchin, U.K.) with a final volume of 20 ml. RCA amplification reaction was performed in a 50 ml mixture containing; 2 ml ligation product, 8 U Bst DNA polymerase (New England Biolabs), 10 pmol of each RCA primer (Table 2) , and 400 mM dNTP mix.


id: cord-000680-vsgd9v1w
author: Lee, Linda K.
date: 2012-06-05
extension: .txt
mime: text/plain
words: 3555
sentence: 164
flesch: 54
cache: cache/cord-000680-vsgd9v1w.txt
txt: txt/cord-000680-vsgd9v1w.txt
title:
  Clinical Relevance and Discriminatory Value of Elevated Liver Aminotransferase Levels for Dengue Severity

summary:
  We excluded severe dengue due to isolated elevation of AST or ALT$1000 U/L from our definition of severe dengue outcome, as this would be a confounder in assessing the relevance of AST or ALT levels in defining dengue severity. Elevation of the liver enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) is common in acute dengue illness, occurring in 65-97% [2, 3, 4, 5] of dengue patients, peaking during the convalescent period of illness (days 7-10)


id: cord-000625-cpjlzutk
author: Ablordey, Anthony
title: Detection of Mycobacterium ulcerans by the Loop Mediated Isothermal Amplification Method
date: 2012-04-03
extension: .txt
mime: text/plain
words: 3678
sentence: 161
flesch: 44
cache: cache/cord-000625-cpjlzutk.txt
txt: txt/cord-000625-cpjlzutk.txt
summary:
  None of the IS2404 PCR negative samples were positive in both types of LAMP assays. Thirty clinical specimens from suspected Buruli ulcer patients were investigated by the modified LAMP (or pocket warmer LAMP) and the conventional LAMP, as well as IS2404 PCR, a reference method for the detection of Mycobacterium ulcerans.


id: cord-276271-3nz3169p
author: Deborggraeve, Stijn
date: 2009-06-02
extension: .txt
mime: text/plain
words: 4736
sentence: 214
flesch: 48
cache: cache/cord-276271-3nz3169p.txt
txt: txt/cord-276271-3nz3169p.txt
title:
  T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease

summary:
  key: cord-276271-3nz3169p authors: Deborggraeve, Stijn; Coronado, Ximena; Solari, Aldo; Zulantay, Ines; Apt, Werner; Mertens, Pascal; Laurent, Thierry; Leclipteux, Thierry; Stessens, Tim; Dujardin, Jean-Claude; Herdewijn, Piet; Büscher, Philippe title: T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease date: 2009-06-02 journal: PLoS Negl Trop Dis DOI: 10.1371/journal.pntd.0000450 sha: doc_id: 276271 cord_uid: 3nz3169p BACKGROUND: PCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. We here present the development and phase I evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA.


id: cord-269004-hj03n13h
author: Krähling, Verena
date: 2010-08-24
extension: .txt
mime: text/plain
words: 4746
sentence: 200
flesch: 48
cache: cache/cord-269004-hj03n13h.txt
txt: txt/cord-269004-hj03n13h.txt
title:
  Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

summary:
  Here, we provide the first insights into this topic by monitoring filovirus infection of a Rousettus aegyptiacus derived cell line, R06E. We were able to show that filoviruses propagate well in R06E cells, which can, therefore, be used to investigate replication and transcription of filovirus RNA and to very efficiently perform rescue of recombinant Marburg virus using reverse genetics. Finally, after having unsuccessfully tested several combinations of cell lines for transfection and passage of potentially rescued virus, we used R06E cells for transfection of plasmids encoding the MARV nucleocapsid proteins as well as the T7 polymerase together with the plasmid containing the full length genome of MARV under the control of the T7 promoter.


id: cord-272250-asuxx1ln
author: Robertson, Kis
title: Rabies-Related Knowledge and Practices Among Persons At Risk of Bat Exposures in Thailand
date: 2011-06-28
extension: .txt
mime: text/plain
words: 4906
sentence: 196
flesch: 45
cache: cache/cord-272250-asuxx1ln.txt
txt: txt/cord-272250-asuxx1ln.txt
summary:
  In this survey among persons at risk for bat exposure in Thailand, we found that although general awareness of rabies transmission and severity were relatively high, awareness of bat rabies in particular was low, with only 10% of participants identifying bats as a potential source of rabies and 36% failing to say they would take any specific action if bitten or scratched by a bat. These findings indicate the need for educational outreach to raise awareness of bat rabies, promote exposure prevention, and ensure appropriate health-seeking behaviors for bat-inflicted wounds, particularly among at-risk groups in Thailand.


id: cord-012911-d6ct94d9
author: Jalloh, Mohamed F.
title: National reporting of deaths after enhanced Ebola surveillance in Sierra Leone
date: 2020-08-18
extension: .txt
mime: text/plain
words: 4844
sentence: 197
flesch: 47
cache: cache/cord-012911-d6ct94d9.txt
txt: txt/cord-012911-d6ct94d9.txt
summary:
  As global health security efforts try to strengthen surveillance systems [32], routine use of death reporting systems like 1-1-7 could play an important role in early detection of clusters of deaths linked to potential infectious disease outbreaks including Ebola. In the telephone survey we identified motivations related to death reporting that have practical implications for improving routine mortality surveillance in a post-Ebola-outbreak setting.


id: cord-270481-rrpqz0uy
author: Hays, Russell
title: Helminth coinfection and COVID-19: An alternate hypothesis
date: 2020-08-17
extension: .txt
mime: text/plain
words: 1081
sentence: 31
flesch: 23
cache: cache/cord-270481-rrpqz0uy.txt
txt: txt/cord-270481-rrpqz0uy.txt
summary:
  key: cord-270481-rrpqz0uy authors: Hays, Russell; Pierce, Doris; Giacomin, Paul; Loukas, Alex; Bourke, Peter; McDermott, Robyn title: Helminth coinfection and COVID-19: An alternate hypothesis date: 2020-08-17 journal: PLoS Negl Trop Dis DOI: 10.1371/journal.pntd.0008628 sha: doc_id: 270481 cord_uid: rrpqz0uy nan In their recently published commentary, Bradbury and colleagues [1] drew attention to the possible negative interactions between helminth infection and COVID-19 severity in helminth-endemic regions. Although the interaction between helminth infection and viral pneumonia is poorly defined, there is some evidence that helminth infection may moderate the process of pulmonary inflammation in viral infections.


id: cord-002581-r7mskri0
author: Magnani, Diogo M.
title: A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus
date: 2017-06-12
extension: .txt
mime: text/plain
words: 5299
sentence: 240
flesch: 50
cache: cache/cord-002581-r7mskri0.txt
txt: txt/cord-002581-r7mskri0.txt
summary:
  Ultrasound Zika Virus Infection and Stillbirths: A Case of Hydrops Fetalis, Hydranencephaly and Fetal Demise Possible Association Between Zika Virus Infection and Microcephaly-Brazil Interim Guidelines for the Evaluation and Testing of Infants with Possible Congenital Zika Virus Infection-United States Interim Guidelines for Pregnant Women During a Zika Virus Outbreak-United States Human antibody responses after dengue virus infection are highly cross-reactive to Zika virus A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virus Molecular determinants of human neutralizing antibodies isolated from a patient infected with Zika virus Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning IgBLAST: an immunoglobulin variable domain sequence analysis tool IMGT/V-QUEST: Zika virus (ZIKV) belongs to the genus Flavivirus of the Flaviviridae family and is related to dengue virus (DENV), yellow fever virus (YFV), Japanese encephalitis virus (JEV), and west Nile virus (WNV)


id: cord-007383-5yb3dxse
author: Kang, Jun-Gu
date: 2020-03-20
extension: .txt
mime: text/plain
words: 5381
sentence: 201
flesch: 42
cache: cache/cord-007383-5yb3dxse.txt
txt: txt/cord-007383-5yb3dxse.txt
title:
  Vaccination with single plasmid DNA encoding IL-12 and antigens of severe fever with thrombocytopenia syndrome virus elicits complete protection in IFNAR knockout mice

summary:
  We examined whether it could provide protective immunity against lethal SFTSV infection in IFNAR KO mice. Vaccination of pSFTSV-IL12 provided complete protection of IFNAR KO mice upon lethal SFTSV challenge, whereas immunization with pSFTSV elicits only partial protection, indicating that antigen-specific cellular immune responses enhanced by co-expression of IL-12 could play a significant role in protection against lethal SFTSV infection.


id: cord-310870-w8wu8vno
author: Shorten, Robert J.
title: The risk of transmission of a viral haemorrhagic fever infection in a United Kingdom laboratory
date: 2017-05-18
extension: .txt
mime: text/plain
words: 1638
sentence: 67
flesch: 37
cache: cache/cord-310870-w8wu8vno.txt
txt: txt/cord-310870-w8wu8vno.txt
summary:
  Laboratory viruses vary between strains and wild-type viruses. All of the high hazard viral diseases cited are caused by enveloped viruses.


id: cord-002394-n85ptr5p
author: Reddy, Vijayalakshmi
date: 2017-01-26
extension: .txt
mime: text/plain
words: 6105
sentence: 247
flesch: 46
cache: cache/cord-002394-n85ptr5p.txt
txt: txt/cord-002394-n85ptr5p.txt
title:
  Molecular Mimicry between Chikungunya Virus and Host Components: A Possible Mechanism for the Arthritic Manifestations

summary:
  Mouse experiments to evaluate the role of CHIKV peptides in causing tissue damage by molecular mimicry On the other hand, mice that received two doses of CHIKV specific peptides but no virus (Groups 3 & 4) exhibited myositis, muscle necrosis, vasculitis and hyperplasia of the marrow (immune mediated inflammatory muscle and marrow reactive changes) and an overall inflammation score of 3+ (Fig 8) .


id: cord-263044-o8aosx2q
author: Lipsitch, Marc
title: Potential Biases in Estimating Absolute and Relative Case-Fatality Risks during Outbreaks
date: 2015-07-16
extension: .txt
mime: text/plain
words: 7019
sentence: 237
flesch: 38
cache: cache/cord-263044-o8aosx2q.txt
txt: txt/cord-263044-o8aosx2q.txt
summary:
  In particular, cases who are not hospitalized, and cases who do not die, may be less likely than other cases to appear in the database because they are less likely to come to medical or public health attention. Returning to the Ebola context, one might wish to know whether pregnant women infected with Ebola are at greater risk of death from Ebola infection than other cases [37] , for example, in order to give them greater supportive care.


id: cord-292157-hrm69640
author: Stull-Lane, Annica R.
date: 2020-10-02
extension: .txt
mime: text/plain
words: 5923
sentence: 256
flesch: 43
cache: cache/cord-292157-hrm69640.txt
txt: txt/cord-292157-hrm69640.txt
title:
  Vitamin A supplementation boosts control of antibiotic-resistant Salmonella infection in malnourished mice

summary:
  Control mice and one group of VAD mice were mock-treated with PBS. Control mice were mock-treated with PBS, one group of VAD mice was treated with vitamin A as previously described and a second group of VAD mice received mock-treatment.


id: cord-003006-lk2ny1wd
author: Cantoni, Diego
title: Ebolaviruses: New roles for old proteins
date: 2018-05-03
extension: .txt
mime: text/plain
words: 6059
sentence: 239
flesch: 41
cache: cache/cord-003006-lk2ny1wd.txt
txt: txt/cord-003006-lk2ny1wd.txt
summary:
  with massive lymphocyte apoptosis Overlapping motifs (PTAP and PPEY) within the Ebola virus VP40 protein function independently as late budding domains: involvement of host proteins TSG101 and VPS-4 Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4 ALIX Rescues Budding of a Double PTAP/PPEY L-Domain Deletion Mutant of Ebola VP40: A Role for ALIX in Ebola Virus Egress Inducing cell proliferation inhibition and apoptosis via silencing Dicer, Drosha, and Exportin 5 in urothelial carcinoma of the bladder Could the Ebola virus matrix protein VP40 be a drug target The natural compound silvestrol is a potent inhibitor of Ebola virus replication Productive replication of Ebola virus is regulated by the c-Abl1 tyrosine kinase The nonstructural small glycoprotein sGP of Ebola virus is secreted as an antiparallel-orientated homodimer A new Ebola virus nonstructural glycoprotein expressed through RNA editing Delta-peptide is the carboxy-terminal cleavage fragment of the nonstructural small glycoprotein sGP of Ebola virus Tyro3 family-mediated cell entry of Ebola and Marburg viruses Ebolavirus glycoprotein structure and mechanism of entry Ebola Virus Entry: A Curious and Complex Series of Events Ebolavirus is internalized into host cells via macropinocytosis in a viral glycoprotein-dependent manner Ebola Virus Enters Host Cells by Macropinocytosis and Clathrin-Mediated Endocytosis Ebola virus and severe acute respiratory syndrome coronavirus display late cell entry kinetics: evidence that transport to NPC1+ endolysosomes is a rate-defining step Ebolavirus Glycoprotein Directs Fusion through NPC1+ Endolysosomes Ebola Virus Glycoprotein with Increased Infectivity Dominated the 2013-2016 Epidemic Human Adaptation of Ebola Virus during the West African Outbreak Functional Characterization of Adaptive Mutations during the West African Ebola Virus Outbreak Spontaneous mutation at amino acid 544 of the Ebola glycoprotein potentiates virus entry and selection in tissue culture Biochemical Basis for Increased Activity of Ebola Glycoprotein in the 2013-2016 Epidemic A Polymorphism within the Internal Fusion Loop of the Ebola Virus Glycoprotein Modulates Host Cell Entry The Ebola virus glycoprotein contributes to but is not sufficient for virulence in vivo Identification of the Ebola virus glycoprotein as the main viral determinant of vascular cell cytotoxicity and injury Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells Ebola virus glycoprotein toxicity is mediated by a dynamin-dependent protein-trafficking pathway The ERK mitogen-activated protein kinase pathway contributes to Ebola virus glycoprotein-induced cytotoxicity Modeling of the Ebola Virus Delta Peptide Reveals a Potential Lytic Sequence Motif Ebola Virus Delta Peptide is a Viroporin Ebola virus glycoprotein GP is not cytotoxic when expressed constitutively at a moderate level Less is more: Ebola virus surface glycoprotein expression levels regulate virus production and infectivity Tetherin-mediated restriction of filovirus budding is antagonized by the Ebola glycoprotein Ebola virus glycoprotein counteracts BST-2/Tetherin restriction in a sequence-independent manner that does not require tetherin surface removal The Tetherin Antagonism of the Ebola Virus Glycoprotein Requires an Intact Receptor-Binding Domain and Can Be Blocked by GP1-Specific Antibodies Ebola Virus Glycoprotein Promotes Enhanced Viral Egress by Preventing Ebola VP40 From Associating With the Host Restriction Factor BST2/Tetherin Effects of Ebola virus glycoproteins on endothelial cell activation and barrier function Ebola Hemorrhagic Fever: Novel Biomarker Correlates of Clinical Outcome Ectodomain shedding of the glycoprotein GP of Ebola virus Shed GP of Ebola Virus Triggers Immune Activation and Increased Vascular Permeability Ebolaviruses associated with differential pathogenicity induce distinct host responses in human macrophages Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection The Myeloid LSECtin Is a DAP12-Coupled Receptor That Is Crucial for Inflammatory Response Induced by Ebola Virus Glycoprotein Antigenic Subversion: A Novel Mechanism of Host Immune Evasion by Ebola Virus Structures of Ebola virus GP and sGP in complex with therapeutic antibodies GP mRNA of Ebola Virus Is Edited by the Ebola Virus Polymerase and by T7 and Vaccinia Virus Polymerases Genomic RNA Editing and Its Impact on Ebola Virus Adaptation During Serial Passages in Cell Culture and Infection of Guinea Pigs Proposal for a revised taxonomy of the family Filoviridae: Classification, names of taxa and viruses, and virus abbreviations Risks Posed by Reston, the Forgotten Ebolavirus The Pathogenesis of Ebola Virus Disease Structural characterization and membrane binding properties of the matrix protein VP40 of Ebola virus The Role of Exosomal VP40 in Ebola Virus Disease Oligomerization and polymerization of the filovirus matrix protein VP40 Structural rearrangement of ebola virus vp40 begets multiple functions in the virus life cycle A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: Implications for filovirus budding Ebola VP40 in Exosomes Can Cause Immune Cell Dysfunction Human fatal zaire ebola virus infection is associated with an aberrant innate immunity and


id: cord-000113-d0eur1hq
author: Fooks, Anthony R.
title: Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in the 21st Century
date: 2009-09-29
extension: .txt
mime: text/plain
words: 6946
sentence: 265
flesch: 34
cache: cache/cord-000113-d0eur1hq.txt
txt: txt/cord-000113-d0eur1hq.txt
summary:
  Atlanta: US Department of Health and Human Services Rabies diagnosis for developing countries The primary application of direct rapid immunohistochemical test to rabies diagnosis in China Evaluation of a direct, rapid immunohistochemical test for rabies diagnosis Simple technique for the collection and shipment of brain specimens for rabies diagnosis Evaluation of a rapid immunodiagnostic test kit for rabies virus Immunochromatographic lateral flow strip tests The polymerase chain reaction (PCR) technique for diagnosis, typing and epidemiological studies Phylogenetic comparison of the genus Lyssavirus using distal coding sequences of the glycoprotein and nucleoprotein genes Identification of regional variants of the rabies virus within the Canadian province of Ontario Polymerase chain reaction protocols for rabies virus discrimination PCR technology for lyssavirus diagnosis Rabies virus detection by RT-PCR in decomposed naturally infected brains Heminested reversetranscriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples Rabies virus in the decomposed brain of an Ethiopian wolf detected by nested reverse transcription-polymerase chain reaction An evaluation of immunofluorescence and PCR methods for detection of rabies in archival Carnoy-fixed, paraffin-embedded brain tissue Usefulness of reverse transcriptasepolymerase chain reaction for detection of rabies RNA in archival samples Molecular diagnosis of animal diseases: some experiences over the past decade PCR technique as an alternative method for diagnosis and molecular epidemiology of rabies virus Molecular methods to distinguish between classical rabies and the rabiesrelated European bat lyssaviruses Experimental infection of big brown bats (Eptesicus fuscus) with Eurasian bat lyssaviruses Aravan, Khujand, and Irkut virus A novel method for real time quantitative RT-PCR Real time quantitative PCR Development of a Real-Time, TaqMan Reverse Transcription-PCR Assay for Detection and Differentiation of Lyssavirus Genotypes 1, 5, and 6 Evaluation of a TaqMan PCR assay to detect rabies virus RNA: influence of sequence variation and application to quantification of viral loads Nucleic-acid sequence based amplification in the rapid diagnosis of rabies Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus Parallel nanoliter detection of cancer markers using polymer microchips Isothermal amplification of rabies virus gene Loop-mediated isothermal amplification of DNA Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus Rapid detection and quantification of Japanese Encephalitis virus by real-time reverse transcription loop-mediated isothermal amplification Novel reverse transcription loopmediated isothermal amplification for rapid detection of foot-and-mouth disease virus Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay Accelerated reaction by loop-mediated isothermal amplification using loop primers Host switching in Lyssavirus history from the Chiroptera to the Carnivora orders Microarray-based detection and genotyping of viral pathogens Microarrays for rapid identification of plant viruses Microassay-based detection of viruses causing vesicular or vesicular-like lesions in livestock animals An ultrasensitive and stable potentiometric immunosensor A novel hepatitis B virus surface antigen immunoassay as sensitive as hepatitis B virus nucleic acid testing in detecting early infection A novel serological assay for the detection of rabies virus neutralising antibodies Development of a TaqMan real-time RT-PCR assay for the detection of rabies virus Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition Bat lyssaviruses (Aravan and Khujand) from Central Asia: phylogenetic relationships according to N, P and G gene sequences Primary structure of leader RNA and nucleoprotein genes of the rabies genome: segmented homology with VSV Case report: rapid ante-mortem diagnosis of a human case of rabies imported into the UK from the Philippines Expert Consultation on Rabies Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies Retroviral pseudotypes Survival after treatment of rabies with induction of coma Applying the Milwaukee Protocol to treat canine rabies in Equatorial Guinea. In the course of the past three decades, the application of molecular biology has aided in the development of tests that result in a more rapid detection of rabies virus.


id: cord-004247-lagv3tp7
author: Hooft van Huijsduijnen, Rob
title: Reassessing therapeutic antibodies for neglected and tropical diseases
date: 2020-01-30
extension: .txt
mime: text/plain
words: 6767
sentence: 279
flesch: 40
cache: cache/cord-004247-lagv3tp7.txt
txt: txt/cord-004247-lagv3tp7.txt
summary:
  In 2 cases, the specific antibodies produced could be identified using B cell cloning [81] or after infection of mice genetically modified to express human antibody repertoires Other recent successes have included new antibodies for use in viral diseases, including HIV.


id: cord-001690-cn21fgug
author: Franceschi, Valentina
date: 2015-06-18
extension: .txt
mime: text/plain
words: 6799
sentence: 239
flesch: 46
cache: cache/cord-001690-cn21fgug.txt
txt: txt/cord-001690-cn21fgug.txt
title:
  BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1((-/-)) Mice from Monkeypoxvirus Lethal Challenge

summary:
  This work demonstrated the efficacy of BoHV-4 based vectors and the use of BoHV-4 as a vaccine-vector platform. In summary, our findings have demonstrated that BoHV-4 based vectors can be used as vaccines to protect against a lethal MPXV challenge in mice.


id: cord-000283-7s6283y5
author: Nazmi, Arshed
date: 2010-11-23
extension: .txt
mime: text/plain
words: 7309
sentence: 289
flesch: 45
cache: cache/cord-000283-7s6283y5.txt
txt: txt/cord-000283-7s6283y5.txt
title:
  Antiviral and Neuroprotective Role of Octaguanidinium Dendrimer-Conjugated Morpholino Oligomers in Japanese Encephalitis

summary:
  Although ROS levels has decreased in JEV+39 MO group when compared to JEV group, it remained significantly higher than that of Sham (p,0.01) ( Figure 5A ). Survivality of animals in each group following JEV infection and Morpholino treatment were monitored daily upto 15 days post JEV infection (or till their death, whichever was earlier).


id: cord-001642-bom9fk1y
author: Wang, Junhua
date: 2015-05-08
extension: .txt
mime: text/plain
words: 6934
sentence: 252
flesch: 41
cache: cache/cord-001642-bom9fk1y.txt
txt: txt/cord-001642-bom9fk1y.txt
title:
  Deletion of Fibrinogen-like Protein 2 (FGL-2), a Novel CD4(+) CD25(+) Treg Effector Molecule, Leads to Improved Control of Echinococcus multilocularis Infection in Mice

summary:
  infected with E. multilocularis, a finding that concurred with other findings demonstrating that E. multilocularis antigens promote T cell differentiation into Treg cells [7] . We then assessed the effect of the targeted deletion of fgl2 on the ability of Treg cells to suppress the proliferation of Teffs.


id: cord-003482-f1uvohf0
author: Malmlov, Ashley
date: 2019-02-04
extension: .txt
mime: text/plain
words: 7514
sentence: 364
flesch: 51
cache: cache/cord-003482-f1uvohf0.txt
txt: txt/cord-003482-f1uvohf0.txt
title:
  Experimental Zika virus infection of Jamaican fruit bats (Artibeus jamaicensis) and possible entry of virus into brain via activated microglial cells

summary:
  Isolation and haemagglutination-inhibition studies on bats collected in Kenya and throughout Uganda Effect of Zika virus and Bwamba virus in the cave bat (Myotis lucifugus) Transmission studies of Hendra virus (equine morbillivirus) in the fruit bats, horses and cats Pteropid bats are confirmed as the reservoir hosts of henipaviruses: A comprehensive experimental study of virus transmission Antibody-mediated immune response in the bat, Pteropus giganteus Detection of specfic antibody responses to vaccinatin in variable flying foxes (Pteropus hypomelanus) The little brown bat, M. lucifugus, displays a highly diverse VH, DH, JH repertoire but little evidence of somatic hypermutation Tacaribe virus cases fatal infection of an ostensible reservoir host, the Jamaican fruit bat Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis) Transcriptomic signatures of tacaribe virus-infected Jamaican fruit bats Assay optimization for molecular detection of Zika virus A rhesus macaque model of Asian-lineage Zika virus infection Zika virus testing considerations: lessons learned from the first eighty real-time RT-PCR-positive cases diagnosed in New York State Detection of Zika virus in urine Long-term kinetics of Zika virus RNA and antibodies in body fluids of a vasectomized traveller returning from Martinique: a case report Persistence of Zika virus in body fluids-Preliminary report Zika virus causes testis damage and leads to male infertility in mice Zika virus infection damages the testes in mice A mouse model of Zika virus pathogenesis Zika viral infection and neutralizing human antibody response in a BLT humanized mouse model Notes from the field: Evidence of Zika virus infection in brain and placental tissues from two congenitally infected newborns and two fetal losses-Brazil Zika virus damages the human placental barrier and presents marked fetal neurotropism Pathology of congenital Zika syndrome in Brazil: a case series Zika virus infection of rhesus macaques leads to viral persistence in multiple tissues Fetal brain lesions after subcutaneous inoculation of Zika virus in a pregnant nonhuman primate Nonhuman primate models of Zika virus infection, immunity, and therapeutic development Zika viral dynamics and shedding in rhesus and cynomolgus macaques Overview of the current status of Zika virus pathogenesis and animal related research Axl mediates Zika virus entry in human glial cells and modulates innate immune responses Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity Entry sites of Venezuelan and western equine encephalitis viruses in the mouse central nervous system following peripheral infection Detection of Zika virus in saliva Biology of Zika virus infection in human skin cells Denge virus in Mexican bats Neotropical bats that co-habit with humans function as dead-end hosts for dengue virus Detection of dengue virus neutralizing antibodies in bats from Costa Rica and Ecuador Sylvatic transmission of arboviruses among bornean orangutans Zika virus, vectors, reservoirs, amplifying hosts, and their potential to spread worldwide: what we know and what we should investigate urgently A sero-epidemiological survey for certain arboviruses (Togaviridae) in Pakistan Investigating the potential role of North American animals as hosts for Zika virus. Distribution of viral antigen in bat tissues suggests that infection in this species recapitulates human infection, which is thought to start with infection of epidermal and dermal cells with subsequent dissemination to multiple organs including salivary glands as viral RNA can be detected in human saliva [44, 45] .


id: cord-002179-v8lpw4r7
author: Viktorovskaya, Olga V.
date: 2016-08-24
extension: .txt
mime: text/plain
words: 8043
sentence: 301
flesch: 41
cache: cache/cord-002179-v8lpw4r7.txt
txt: txt/cord-002179-v8lpw4r7.txt
title:
  Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements

summary:
  [13] exploited a cross-linking label-free MS approach to identify DENV RNA associating proteins in cell culture by cross-linking the RNA to the proteins using short wavelength UV light and isolating DENV RNA bound proteins by anti-sense DNA affinity capture [74] . The overall down-regulation of RNA binding proteins appears to be driven by changes in cytoplasmic and mitochondrial ribosomal subunits, and proteins involved in RNA degradation and processing (S5 Fig).


id: cord-001074-qevosik3
author: Selvarajah, Suganya
date: 2013-09-12
extension: .txt
mime: text/plain
words: 7646
sentence: 304
flesch: 47
cache: cache/cord-001074-qevosik3.txt
txt: txt/cord-001074-qevosik3.txt
title:
  A Neutralizing Monoclonal Antibody Targeting the Acid-Sensitive Region in Chikungunya Virus E2 Protects from Disease

summary:
  Shotgun mutagenesis alanine scanning of 98 percent of the residues in the E1 and E2 glycoproteins of CHIKV envelope showed that the epitope bound by C9 included amino-acid 162 in the acid-sensitive region (ASR) of the CHIKV E2 glycoprotein. The ASR is critical for the rearrangement of CHIKV E2 during fusion and viral entry into host cells, and we predict that C9 prevents these events from occurring.


id: cord-308343-crjjhpl1
author: Graef, Geneva
title: Impact of confinement housing on study end-points in the calf model of cryptosporidiosis
date: 2018-04-25
extension: .txt
mime: text/plain
words: 8082
sentence: 341
flesch: 49
cache: cache/cord-308343-crjjhpl1.txt
txt: txt/cord-308343-crjjhpl1.txt
summary:
  The Wilcoxon Rank Sum was used to assess differences in fecal oocyst counts and fecal dry matter percentage between IC and CFC samples for confinement housing calves and between IC samples in confinement housing and box stall calves. The mean peak in oocyst shedding for confinement housing calves was log 7.5 oocysts/ gram fecal dry matter, which is nearly 3 orders of magnitude greater than the mean peak in box stall calves.


id: cord-322943-lvdl7puw
author: Lardon, Zélie
date: 2010-06-22
extension: .txt
mime: text/plain
words: 4460
sentence: 167
flesch: 39
cache: cache/cord-322943-lvdl7puw.txt
txt: txt/cord-322943-lvdl7puw.txt
title:
  Imported Episodic Rabies Increases Patient Demand for and Physician Delivery of Antirabies Prophylaxis

summary:
  Found at: doi:10.1371/journal.pntd.0000723.s001 (0.07 MB DOC) Best practices in public health risk and crisis communication Communicating the threat of emerging infections to the public Six propositions on public participation and their relevance for risk communication The public's response to severe acute respiratory syndrome in Toronto and the United States Communicating the risks of a new, emerging pathogen: the case of Cryptococcus gattii Rabies and other lyssavirus diseases Estimating the public health impact of rabies Rabies in Europe in 2005 Expert consultation on rabies Epidemiology and prophylaxis of rabies in humans in France: evaluation and perspectives of a twenty-five year surveillance programme A reliable diagnosis of human rabies based on analysis of skin biopsy specimens Rabies vaccines What is an acceptable delay in rabies immune globulin administration when vaccine alone had been given previously Time series analysis : forecasting and control Revue méthodologique de quelques techniques spécifiques à l'analyse des séries temporelles en épidémiologie et santé publique A time series construction of an alert threshold with application to S. bobimorbificans in France The theory and pratice of econometrics Que penser de la rage en 1990? The constant media attention drawn by these different players during event #6 may have contributed to enhancing the sense of rabies risk, thereby prompting people to associate dog bites with We only examined national newspaper stories available in Factiva but not local newspaper reporting or television, radio and internet stories, and, thus, probably underestimated the global coverage of these episodes.


id: cord-002248-92pzqj35
author: Terasaki, Kaori
date: 2016-10-06
extension: .txt
mime: text/plain
words: 8551
sentence: 316
flesch: 48
cache: cache/cord-002248-92pzqj35.txt
txt: txt/cord-002248-92pzqj35.txt
title:
  Mechanistic Insight into the Host Transcription Inhibition Function of Rift Valley Fever Virus NSs and Its Importance in Virulence

summary:
  (TIF) Breaking the chain: Rift Valley fever virus control via livestock vaccination The Pathogenesis of Rift Valley Fever NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture Deletion of the NSm virulence gene of Rift Valley fever virus inhibits virus replication in and dissemination from the midgut of Aedes aegypti mosquitoes The Rift Valley fever accessory proteins NSm and P78/NSm-GN are distinct determinants of virus propagation in vertebrate and invertebrate hosts NSm protein of Rift Valley fever virus suppresses virusinduced apoptosis The C-terminal region of Rift Valley fever virus NSm protein targets the protein to the mitochondrial outer membrane and exerts antiapoptotic function Recent advances in the molecular and cellular biology of bunyaviruses The Rift Valley fever virus nonstructural protein NSs is phosphorylated at serine residues located in casein kinase II consensus motifs in the carboxy-terminus The carboxy-terminal acidic domain of Rift Valley Fever virus NSs protein is essential for the formation of filamentous structures but not for the nuclear localization of the protein A SAP30 complex inhibits IFNbeta expression in Rift Valley fever virus infected cells TFIIH transcription factor, a target for the Rift Valley hemorrhagic fever virus NSs protein of rift valley fever virus promotes posttranslational downregulation of the TFIIH subunit p62 Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH Rift Valley fever virus NSs protein promotes post-transcriptional downregulation of protein kinase PKR and inhibits eIF2alpha phosphorylation NSs protein of rift valley fever virus induces the specific degradation of the double-stranded RNA-dependent protein kinase Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF) FBXW11-NSs E3 Ligase NSs virulence factor of Rift Valley fever virus engages the F-box proteins FBXW11 and beta-TRCP1 to degrade the antiviral protein kinase PKR Reactive oxygen species activate NFkappaB (p65) and p53 and induce apoptosis in RVFV infected liver cells Induction of DNA damage signaling upon Rift Valley fever virus infection results in cell cycle arrest and increased viral replication p53 Activation following Rift Valley fever virus infection contributes to cell death and viral production Nonstructural NSs protein of rift valley fever virus interacts with pericentromeric DNA sequences of the host cell, inducing chromosome cohesion and segregation defects Functional analysis of Rift Valley fever virus NSs encoding a partial truncation Rescue of infectious rift valley fever virus entirely from cDNA, analysis of virus lacking the NSs gene, and expression of a foreign gene Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development NIH Image to ImageJ: 25 years of image analysis Rift valley fever virus nonstructural protein NSs promotes viral RNA replication and transcription in a minigenome system Severe acute respiratory syndrome coronavirus nsp1 suppresses host gene expression, including that of type I interferon, in infected cells Mechanism of tripartite RNA genome packaging in Rift Valley fever virus Characterization of clone 13, a naturally attenuated avirulent isolate of Rift Valley fever virus, which is altered in the small segment A ΩXaV motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence Recombinant Rift Valley fever vaccines induce protective levels of antibody in baboons and resistance to lethal challenge in mice Interplay between the Virus and Host in Rift Valley Fever Pathogenesis Host alternation is necessary to maintain the genome stability of rift valley fever virus We thank Robert Tesh for anti-MP12 antibody; Paul Gottlieb for the monoclonal antibody H2K k D k ; Mark Griffin (Flow Cytometry and Cell Sorting Core, The University of Texas Medical Branch) for support with the flow cytometry analyses; and Krishna Narayanan for critical reading of the manuscript. Rift Valley fever virus (RVFV), a member of the genus Phlebovirus within the family Bunyaviridae, causes periodic outbreaks in livestocks and humans in countries of the African continent and Middle East.


id: cord-001812-ov1qssnu
author: Fan, Yi-Chin
date: 2015-10-23
extension: .txt
mime: text/plain
words: 8339
sentence: 282
flesch: 37
cache: cache/cord-001812-ov1qssnu.txt
txt: txt/cord-001812-ov1qssnu.txt
title:
  Formalin Inactivation of Japanese Encephalitis Virus Vaccine Alters the Antigenicity and Immunogenicity of a Neutralization Epitope in Envelope Protein Domain III

summary:
  Cochrane Database Syst Rev: CD004263 Intranasal immunization with formalininactivated virus vaccine induces a broad spectrum of heterosubtypic immunity against influenza A virus infection in mice A controlled trial of a formalin-inactivated hepatitis A vaccine in healthy children A formalin-inactivated whole SIV vaccine confers protection in macaques Local antibody response to poliovaccine in the human female genital tract Anti-Rabies Vaccine of Tissue Culture Origin Technical aspects of immunohistochemistry Identification of formaldehyde-induced modifications in proteins: reactions with model peptides Effect of inactivation method on the cross-protective immunity induced by whole 'killed' influenza A viruses and commercial vaccine preparations Antigenic characterization of a formalin-inactivated poliovirus vaccine derived from live-attenuated Sabin strains Effects of formalin inactivation on bovine herpes virus-1 glycoproteins and antibody response elicited by formalin-inactivated vaccines in rabbits Antigenic structure of hepatitis B surface antigen: identification of the d subtype determinant by chemical modification and use of monoclonal antibodies Effect of formaldehyde inactivation on poliovirus Immunogenicity and protective efficacy in mice of a formaldehyde-inactivated Indian strain of Japanese encephalitis virus grown in Vero cells Potential role of Armigeres subalbatus (Diptera: Culicidae) in the transmission of Japanese encephalitis virus in the absence of rice culture on Liu-chiu islet Japanese encephalitis virus genotype replacement Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies Localization and characterization of flavivirus envelope glycoprotein crossreactive epitopes Enzyme-linked immunosorbent assays using novel Japanese encephalitis virus antigen improve the accuracy of clinical diagnosis of flavivirus infections A single intramuscular injection of recombinant plasmid DNA induces protective immunity and prevents Japanese encephalitis in mice A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen Differentiation of West Nile and St. Louis encephalitis virus infections by use of noninfectious virus-like particles with reduced cross-reactivity Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein Mass spectrometric identification of formaldehydeinduced peptide modifications under in vivo protein cross-linking conditions Structural basis of West Nile virus neutralization by a therapeutic antibody A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interacted with neutralizing-antibody combining sites Host cell selection of Murray Valley encephalitis virus variants altered at an RGD sequence in the envelope protein and in mouse virulence Probing the flavivirus membrane fusion mechanism by using monoclonal antibodies Humoral immune responses of dengue fever patients using epitope-specific serotype-2 virus-like particle antigens The anamnestic neutralizing antibody response is critical for protection of mice from challenge following vaccination with a plasmid encoding the Japanese encephalitis virus premembrane and envelope genes UV-inactivated vaccinia virus (VV) in a multi-envelope DNA-VV-protein (DVP) HIV-1 vaccine protects macaques from lethal challenge with heterologous SHIV A subcutaneously injected UVinactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice Development of a new hydrogen peroxide-based vaccine platform Antiseptics and disinfectants: activity, action, and resistance Feasibility of cross-protective vaccination against flaviviruses of the Japanese encephalitis serocomplex New Japanese encephalitis vaccines: alternatives to production in mouse brain Recent advancement in flavivirus vaccine development Japanese encephalitis: the need for a more effective vaccine A potential molecular mechanism for hypersensitivity caused by formalin-inactivated vaccines Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein Poorly neutralizing cross-reactive antibodies against the fusion loop of West Nile virus envelope protein protect in vivo via Fcgamma receptor and complement-dependent effector mechanisms Immunodominance and functional activities of antibody responses to inactivated West Nile virus and recombinant subunit vaccines in mice Immunogenicity and efficacy of two types of West Nile virus-like particles different in size and maturation as a second-generation vaccine candidate High antibody prevalence in an unconventional ecosystem is related to circulation of a low-virulent strain of Japanese encephalitis virus Homologous and heterologous neutralization antibody responses after immunization with Japanese encephalitis vaccine among Taiwan children Manipulation of immunodominant dengue virus E protein epitopes reduces potential antibody-dependent enhancement Sculpting humoral immunity through dengue vaccination to enhance protective immunity Protective mechanisms induced by a Japanese encephalitis virus DNA vaccine: requirement for antibody but not CD8(+) cytotoxic T-cell responses Immunization with plasmid DNA encoding the envelope glycoprotein of Japanese Encephalitis virus confers significant protection against intracerebral viral challenge without inducing detectable antiviral antibodies Envelope deglycosylation enhances antigenicity of HIV-1 gp41 epitopes for both broad neutralizing antibodies and their unmutated ancestor antibodies Elicitation of structure-specific antibodies by epitope scaffolds Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection Oxidation of virus proteins during UV(254) and singlet oxygen mediated inactivation UV-photolysis of amino acids and peptides. To rule out the potential influence of sub-strain differences on the E structure and focus on the effect of formalin treatment on antigenic modification of Nakayama virus, we subjected laboratory-grown concentrated Nakayama virus to either formalin inactivation (FIV-Nakayama) or without formalin at 4°C for 49 days (untreated control virus-Nakayama virus, UCV-Nakayama).


id: cord-001365-6u80p5sj
author: Weger-Lucarelli, James
title: A Novel MVA Vectored Chikungunya Virus Vaccine Elicits Protective Immunity in Mice
date: 2014-07-24
extension: .txt
mime: text/plain
words: 8483
sentence: 342
flesch: 46
cache: cache/cord-001365-6u80p5sj.txt
txt: txt/cord-001365-6u80p5sj.txt
summary:
  For the production of recombinant MVA vaccines, the CHIKV E2 and E3 genes (hereafter called p62) were PCR amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and cloned into a specially modified poxvirus transfer vector, pI2-Red [37] . Green represents CHIKV E2 positive cells, red is dsRed protein produced by the virus and blue is nuclear staining with Hoechst.


id: cord-003389-0yh5k6jk
author: Patton, John B.
date: 2018-12-12
extension: .txt
mime: text/plain
words: 8003
sentence: 311
flesch: 45
cache: cache/cord-003389-0yh5k6jk.txt
txt: txt/cord-003389-0yh5k6jk.txt
title:
  Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum

summary:
  The immunodeficient scid mouse as a model for human lymphatic filariasis Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells Defects in the differentiation and function of antigen presenting cells in NOD/Lt mice Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans The SCID mouse mutant: definition, characterization, and potential uses Expanded CD34+ human umbilical cord blood cells generate multiple lymphohematopoietic lineages in NOD-scid IL2rgamma(null) mice Methylprednisolone acetate induces, and Delta7-dafachronic acid suppresses, Strongyloides stercoralis hyperinfection in NSG mice Non-obese diabetic-recombination activating gene-1 (NOD-Rag1 null) interleukin (IL)-2 receptor common gamma chain (IL2r gamma null) null mice: a radioresistant model for human lymphohaematopoietic engraftment Eosinophils and IL-4 Support Nematode Growth Coincident with an Innate Response to Tissue Injury Humoral immunity in humanized mice: a work in progress AML xenograft efficiency is significantly improved in NOD/SCID-IL2RG mice constitutively expressing human SCF, GM-CSF and IL-3 BLT humanized mice as a small animal model of HIV infection Ivermectin resistance in Onchocerca volvulus: toward a genetic basis Genome-wide analysis of ivermectin response by Onchocerca volvulus reveals that genetic drift and soft selective sweeps contribute to loss of drug sensitivity Protective immunity to the larval stages of Onchocerca volvulus is dependent on Toll-like receptor 4 Immunoglobulin E and eosinophil-dependent protective immunity to larval Onchocerca volvulus in mice immunized with irradiated larvae Differential cytokine and antibody responses to adult and larval stages of Onchocerca volvulus consistent with the development of concomitant immunity Cryopreservation of infective larvae of Onchocerca volvulus (Filarioidea: Onchocercidae) Infection of tissue culture cells with bloodstream trypomastigotes of Trypanosoma cruzi Detection of Onchocerca volvulus infection by O-150 polymerase chain reaction analysis of skin scratches We hypothesized that highly immunodeficient NSG mice would support the survival and maturation of O. volvulus and alteration of the host microenvironment through the addition of various human cells and tissues would further enhance the level of parasite maturation.


id: cord-003243-u744apzw
author: Michael, Edwin
date: 2018-10-08
extension: .txt
mime: text/plain
words: 10328
sentence: 280
flesch: 27
cache: cache/cord-003243-u744apzw.txt
txt: txt/cord-003243-u744apzw.txt
title:
  Quantifying the value of surveillance data for improving model predictions of lymphatic filariasis elimination

summary:
  key: cord-003243-u744apzw authors: Michael, Edwin; Sharma, Swarnali; Smith, Morgan E.; Touloupou, Panayiota; Giardina, Federica; Prada, Joaquin M.; Stolk, Wilma A.; Hollingsworth, Deirdre; de Vlas, Sake J. title: Quantifying the value of surveillance data for improving model predictions of lymphatic filariasis elimination date: 2018-10-08 journal: PLoS Negl Trop Dis DOI: 10.1371/journal.pntd.0006674 sha: doc_id: 3243 cord_uid: u744apzw BACKGROUND: The present results further pinpoint the crucial need for longitudinal infection surveillance data for enhancing the precision and accuracy of model predictions of the intervention durations required to achieve parasite elimination in an endemic location.


id: cord-340569-f1odmjcs
author: Hossain, Mohammad Sorowar
title: Dengue in a crowded megacity: Lessons learnt from 2019 outbreak in Dhaka, Bangladesh
date: 2020-08-20
extension: .txt
mime: text/plain
words: 1355
sentence: 50
flesch: 45
cache: cache/cord-340569-f1odmjcs.txt
txt: txt/cord-340569-f1odmjcs.txt
summary:
  While the incidence of dengue cases decreased sharply in Dhaka city after the Eid week (as much as about 24 times compared to pre-Eid week), a net increase (around 4--7-fold) was observed in some districts of Bangladesh, including Narail, Pirojpur, Manikgonj, and Faridpur (Fig 2) . Time series cross-correlation and autoregressive model showed that the dengue within Dhaka city appeared to play a significant role in the transmission of dengue outside of Dhaka (S1 Fig, S1 Table) .


id: cord-345315-y3bdjnhg
author: Dai, Yaoyao
date: 2020-10-01
extension: .txt
mime: text/plain
words: 3331
sentence: 132
flesch: 47
cache: cache/cord-345315-y3bdjnhg.txt
txt: txt/cord-345315-y3bdjnhg.txt
title:
  Identifying the outbreak signal of COVID-19 before the response of the traditional disease monitoring system

summary:
  [18] Timeliness and precision in the detection of infectious disease outbreaks from the information published on the web are crucial for prevention of their spread. Insights from different clinical settings Identification of terms for detecting early signals of emerging infectious disease outbreaks on the web. Computers and Electronics in Agriculture Use of Google Trends to investigate loss-of-smell-related searches during the COVID-19 outbreak Retrospective analysis of the possibility of predicting the COVID-19 outbreak from Internet searches and social media data, China, 2020.


id: cord-340939-ikomc19t
author: van Doremalen, Neeltje
date: 2019-06-06
extension: .txt
mime: text/plain
words: 5494
sentence: 227
flesch: 46
cache: cache/cord-340939-ikomc19t.txt
txt: txt/cord-340939-ikomc19t.txt
title:
  A single-dose ChAdOx1-vectored vaccine provides complete protection against Nipah Bangladesh and Malaysia in Syrian golden hamsters

summary:
  A time-and cost-efficient system for high-level protein production in mammalian cells Biochemical, conformational, and immunogenic analysis of soluble trimeric forms of henipavirus fusion glycoproteins RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues Comparison of the pathogenicity of Nipah virus isolates from Bangladesh and Malaysia in the Syrian hamster A VLP-based vaccine provides complete protection against Nipah virus challenge following multiple-dose or single-dose vaccination schedules in a hamster model Recombinant nipah virus vaccines protect pigs against challenge ChAdOx1 NiVB efficacy in the Syrian Golden hamster PLOS Neglected Tropical Diseases Protection against henipaviruses in swine requires both, cell-mediated and humoral immune response A Hendra virus G glycoprotein subunit vaccine protects African green monkeys from Nipah virus challenge Single-dose live-attenuated vesicular stomatitis virus-based vaccine protects African green monkeys from Nipah virus disease Recombinant measles virus vaccine expressing the Nipah virus glycoprotein protects against lethal Nipah virus challenge A recombinant subunit vaccine formulation protects against lethal Nipah virus challenge in cats Single injection recombinant vesicular stomatitis virus vaccines protect ferrets against lethal Nipah virus disease Vaccination of ferrets with a recombinant G glycoprotein subunit vaccine provides protection against Nipah virus disease for over 12 months Nipah virus: vaccination and passive protection studies in a hamster model Characterization of Nipah virus from naturally infected Pteropus vampyrus bats Isolation of Nipah virus from Malaysian Island flying-foxes Nipah virus in Lyle's flying foxes, Cambodia Protection against henipavirus infection by use of recombinant adeno-associated virus-vector vaccines Feline model of acute nipah virus infection and protection with a soluble glycoprotein-based subunit vaccine Neutralization assays for differential henipavirus serology using Bio-Plex protein array systems Changing resource landscapes and spillover of henipaviruses Hendra virus vaccine, a one health approach to protecting horse, human, and environmental health A neutralizing human monoclonal antibody protects against lethal disease in a new ferret model of acute nipah virus infection A neutralizing human monoclonal antibody protects african green monkeys from hendra virus challenge A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus Development of an acute and highly pathogenic nonhuman primate model of Nipah virus infection We would like to thank the animal care takers for their excellent care of the animals, and Anita Mora for assistance with figures. The Syrian hamster is a suitable initial small animal model to investigate the efficacy of NiV vaccines, followed by the African green monkey


id: cord-327799-ngzvdd8c
author: Chaumont, Claire
title: The SARS-CoV-2 crisis and its impact on neglected tropical diseases: Threat or opportunity?
date: 2020-09-21
extension: .txt
mime: text/plain
words: 1798
sentence: 78
flesch: 42
cache: cache/cord-327799-ngzvdd8c.txt
txt: txt/cord-327799-ngzvdd8c.txt
summary:
  Human resources for NTD activities are thus shifting due to economic crisis and health system priorities. In parallel, there is an opportunity to try different delivery models to ensure the success of NTD activities restarting while mitigating potential risks.


id: cord-280030-neqycg6v
author: Sewlall, Nivesh H.
title: Clinical Features and Patient Management of Lujo Hemorrhagic Fever
date: 2014-11-13
extension: .txt
mime: text/plain
words: 6568
sentence: 294
flesch: 46
cache: cache/cord-280030-neqycg6v.txt
txt: txt/cord-280030-neqycg6v.txt
summary:
  I. Clinical description and pathological findings A prospective study of the epidemiology and ecology of Lassa fever Aerosol generating procedures and risk of transmission of acute respiratory infections to healthcare workers: a systematic review An outbreak of Lassa fever on the Jos plateau Review of cases of nosocomial Lassa fever in Nigeria: the high price of poor medical practice Lassa fever in France Ebola Hemorrhagic Fever: Novel Biomarker Correlates of Clinical Outcome 389: Viral Hemorrhagic Fevers Treatment of Marburg and Ebola hemorrhagic fevers: a strategy for testing new drugs and vaccines under outbreak conditions Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock Treatment of Bolivian hemorrhagic fever with intravenous ribavirin Brief report: treatment of a laboratory-acquired Sabia virus infection Treatment of Argentine hemorrhagic fever Review of the literature and proposed guidelines for the use of oral ribavirin as postexposure prophylaxis for Lassa fever Mechanism of action and value of N-acetylcysteine in the treatment of early and late acetaminophen poisoning: a critical review The effect of N-acetylcysteine on nuclear factor-kappa B activation, interleukin-6, interleukin-8, and intercellular adhesion molecule-1 expression in patients with sepsis The activation of factor X and prothrombin by recombinant factor VIIa in vivo is mediated by tissue factor Efficacy and safety of recombinant factor VIIa for treatment of severe bleeding: a systematic review Low-dose recombinant factor VIIa for trauma patients with coagulopathy Simvastatin decreases nitric oxide overproduction and reverts the impaired vascular responsiveness induced by endotoxic shock in rats Highvolume hemofiltration as salvage therapy in severe hyperdynamic septic shock Severe hemorrhagic fever in strain 13/N guinea pigs infected with Lujo virus New opportunities for field research on the pathogenesis and treatment of Lassa fever Lassa fever in Guinea: I. Epidemiology of human disease and clinical observations Some distinctive features of LHF relative to typical Lassa fever were the abrupt disease onset (typically indolent in Lassa fever) and the presence of DIC, which is generally not considered to be part of the pathogenesis of Lassa fever, although the matter has not been extensively studied [9] .


id: cord-303647-c4umbcvn
author: Reed, Patricia E.
title: A New Approach for Monitoring Ebolavirus in Wild Great Apes
date: 2014-09-18
extension: .txt
mime: text/plain
words: 5645
sentence: 199
flesch: 40
cache: cache/cord-303647-c4umbcvn.txt
txt: txt/cord-303647-c4umbcvn.txt
summary:
  This study represents the first time that ebolavirus antibodies have been detected in wild great ape fecal samples, and carries important implications for the future management and survival of these primates. We also tested ape fecal samples obtained from the outbreakfree (NRO) region to explore whether NP antibody detection can be used as a potential surveillance tool.


id: cord-276850-tnlyk0wz
author: Rodrigues, Anderson Messias
date: 2015-08-25
extension: .txt
mime: text/plain
words: 5941
sentence: 244
flesch: 40
cache: cache/cord-276850-tnlyk0wz.txt
txt: txt/cord-276850-tnlyk0wz.txt
title:
  Proteomics-Based Characterization of the Humoral Immune Response in Sporotrichosis: Toward Discovery of Potential Diagnostic and Vaccine Antigens

summary:
  S. brasiliensis proteins were therefore resolved via 2D electrophoresis and immunoblotted with pooled sera from cats with sporotrichosis (n = 10) and optimal antibody titers according to ELISA. As expected, antibodies from cats with sporotrichosis reacted with a wide variety of S. brasiliensis and S. schenckii proteins 20kDa to >160kDa in size (Fig 3) .


id: cord-294798-ji3p0l4j
author: White, Sarah K.
date: 2018-05-31
extension: .txt
mime: text/plain
words: 4796
sentence: 161
flesch: 41
cache: cache/cord-294798-ji3p0l4j.txt
txt: txt/cord-294798-ji3p0l4j.txt
title:
  Detection and phylogenetic characterization of arbovirus dual-infections among persons during a chikungunya fever outbreak, Haiti 2014

summary:
  As part of ongoing screening of schoolchildren presenting with acute undifferentiated febrile illness in rural Haiti, we used RT-PCR to identify CHIKV infections in 82 of 100 children with this diagnosis during May—August 2014. The acute symptoms of CHIKV infection are similar to those of infection with other arbovirus species, including Dengue virus (DENV), Zika virus (ZIKV), and Mayaro virus (MAYV), each presenting with a constellation of symptoms including fever, headache, and myalgias/arthralgias.


id: cord-285772-4xt4anq5
author: Huang, Rui
date: 2020-05-08
extension: .txt
mime: text/plain
words: 3382
sentence: 184
flesch: 56
cache: cache/cord-285772-4xt4anq5.txt
txt: txt/cord-285772-4xt4anq5.txt
title:
  Clinical findings of patients with coronavirus disease 2019 in Jiangsu province, China: A retrospective, multi-center study

summary:
  The age of severe patients was comparable with non-severe patients (median age 49.0 yr vs. 44.0 yr, P = 0.066) and the BMI index of severe patients was higher than non-severe patients (median BMI, 26.4 kg/m 2 vs. 24.2 kg/m 2 , P = 0.004). [ Compared to non-severe patients, severe patients presented higher percentage of shortness of breath (7.3% vs. 26.1%, P = 0.004), lower lymphocytes (median 0.8 ×10 9 /L vs. 1.2×10 9 /L, P<0.001) and albumin (ALB) levels (median 38.1 g/L vs. 41.1 g/L, P = 0.002).


id: cord-355469-ojop6i4k
author: Egawa, Kazutaka
date: 2017-12-14
extension: .txt
mime: text/plain
words: 5349
sentence: 234
flesch: 57
cache: cache/cord-355469-ojop6i4k.txt
txt: txt/cord-355469-ojop6i4k.txt
title:
  Virulence, pathology, and pathogenesis of Pteropine orthoreovirus (PRV) in BALB/c mice: Development of an animal infection model for PRV

summary:
  Although all of the cases of PRV infection in humans showed symptoms associated with RTI, it is evident that the clinical characteristics of PRV infections in humans have not been fully elucidated. PRV caused lethal respiratory disease in an animal model of PRV infection, in which BALB/c mice were used.


id: cord-281456-3dlsbr7c
author: Al-alimi, Abdullah Ahmed
date: 2014-03-13
extension: .txt
mime: text/plain
words: 4866
sentence: 201
flesch: 43
cache: cache/cord-281456-3dlsbr7c.txt
txt: txt/cord-281456-3dlsbr7c.txt
title:
  Dengue Virus Type 2 (DENV2)-Induced Oxidative Responses in Monocytes from Glucose-6-Phosphate Dehydrogenase (G6PD)-Deficient and G6PD Normal Subjects

summary:
  After this peak, the percentage of DENV2 infected cells decreased (Fig. 2A) . In normal control monocytes culture, the frequency of DENV2 infected cells increased until 72 hours post-infection, when peak values were reached followed by a decrease (Fig. 2A) .


id: cord-305890-mdwjrfzp
author: Bönsch, Claudia
title: Chloroquine and Its Derivatives Exacerbate B19V-Associated Anemia by Promoting Viral Replication
date: 2010-04-27
extension: .txt
mime: text/plain
words: 4130
sentence: 190
flesch: 47
cache: cache/cord-305890-mdwjrfzp.txt
txt: txt/cord-305890-mdwjrfzp.txt
summary:
  The aims of this study were to assess the effect of CQ and other common antimalarial drugs on B19V infection in vitro and the possible epidemiological consequences for children from Papua New Guinea (PNG). CQ facilitates B19V infection by minimizing intracellular degradation of incoming particles.


id: cord-288202-r3r2bc7v
author: Morel, Noelia
date: 2013-01-10
extension: .txt
mime: text/plain
words: 5128
sentence: 174
flesch: 40
cache: cache/cord-288202-r3r2bc7v.txt
txt: txt/cord-288202-r3r2bc7v.txt
title:
  A Monoclonal Antibody-Based Copro-ELISA Kit for Canine Echinococcosis to Support the PAHO Effort for Hydatid Disease Control in South America

summary:
  Although the rate of success has been highly variable, it has become evident that a tight control of dog infections is the key element to arrest the life cycle of the parasite. However, accurate diagnosis of dog infection is complex and challenging, and other than careful necropsy of dogs, there is no perfect gold standard [6] .


id: cord-332473-ec8lu2a3
author: Amorim, Raquel
title: Zika virus inhibits eIF2α-dependent stress granule assembly
date: 2017-07-17
extension: .txt
mime: text/plain
words: 6570
sentence: 285
flesch: 48
cache: cache/cord-332473-ec8lu2a3.txt
txt: txt/cord-332473-ec8lu2a3.txt
summary:
  ZIKV is a positive-strand RNA virus that replicates in the cytoplasm but little is known about redistribution of host proteins in ZIKV infected cells. In contrast, ZIKV infected cells presented SG at a rate of only 21.6% (Fig 2A, bottom panel and Fig 2B) .


id: cord-309587-xc4jaw31
author: Lembo, Tiziana
title: The Feasibility of Canine Rabies Elimination in Africa: Dispelling Doubts with Data
date: 2010-02-23
extension: .txt
mime: text/plain
words: 5989
sentence: 228
flesch: 36
cache: cache/cord-309587-xc4jaw31.txt
txt: txt/cord-309587-xc4jaw31.txt
summary:
  As a result, not only has dog rabies declined, but human rabies deaths have also been eliminated, or cases remain highly localized [5] . The contrast with the situation in Africa and Asia is striking; here, the incidence of dog rabies and human rabies deaths continue to escalate, and new outbreaks have been occurring in areas previously free of the disease (e.g. the islands of Flores and Bali in Indonesia -


id: cord-286255-ded5t1ai
author: Tomashek, Kay M.
date: 2017-09-13
extension: .txt
mime: text/plain
words: 6148
sentence: 263
flesch: 50
cache: cache/cord-286255-ded5t1ai.txt
txt: txt/cord-286255-ded5t1ai.txt
title:
  Clinical and epidemiologic characteristics of dengue and other etiologic agents among patients with acute febrile illness, Puerto Rico, 2012–2015

summary:
  A higher proportion of participants with dengue reported having a household member with dengue at enrollment than participants with other diagnoses (11.8% of dengue cases versus 5% in other diagnostic groups, p <0.001). Participants with laboratory-positive dengue presented later (median = 3 days), and a higher proportion were admitted at enrollment than participants with other laboratory diagnoses; nearly half (46.6%) of dengue cases were admitted compared with 27.3% of participants with ORV illness, 18.8% with influenza, and 11.2% with chikungunya.


id: cord-350533-fp1ctpax
author: Tchesnokov, Egor P.
date: 2020-06-04
extension: .txt
mime: text/plain
words: 6066
sentence: 278
flesch: 46
cache: cache/cord-350533-fp1ctpax.txt
txt: txt/cord-350533-fp1ctpax.txt
title:
  Independent inhibition of the polymerase and deubiquitinase activities of the Crimean-Congo Hemorrhagic Fever Virus full-length L-protein

summary:
  Recent advances in understanding Crimean-Congo hemorrhagic fever virus Crimean-Congo hemorrhagic fever and expansion from endemic regions Crimean-Congo haemorrhagic fever in travellers: A systematic review The WHO R&D Blueprint: 2018 review of emerging infectious diseases requiring urgent research and development efforts Biochemical Evaluation of the Inhibition Properties of Favipiravir and 2'-C-Methyl-Cytidine Triphosphates against Human and Mouse Norovirus RNA Polymerases The ambiguous base-pairing and high substrate efficiency of T-705 (Favipiravir) Ribofuranosyl 5'-triphosphate towards influenza A virus polymerase Mechanism of action of T-705 against influenza virus Japanese Surveillance Systems and Treatment for Influenza The broad-spectrum antiviral ribonucleoside ribavirin is an RNA virus mutagen Crimean-Congo hemorrhagic fever virusencoded ovarian tumor protease activity is dispensable for virus RNA polymerase function Favipiravir (T-705) but not ribavirin is effective against two distinct strains of Crimean-Congo hemorrhagic fever virus in mice Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever Favipiravir (T-705), a broad spectrum inhibitor of viral RNA polymerase The efficacy of ribavirin in the treatment of Crimean-Congo hemorrhagic fever in Eastern Black Sea region in Turkey Identification of 2'-deoxy-2'-fluorocytidine as a potent inhibitor of Crimean-Congo hemorrhagic fever virus replication using a recombinant fluorescent reporter virus Inhibition of the subgenomic hepatitis C virus replicon in huh-7 cells by 2'-deoxy-2'-fluorocytidine Bunyaviridae RdRps: structure, motifs, and RNA synthesis machinery Transcription and replication mechanisms of Bunyaviridae and Arenaviridae L proteins Structural insight into cap-snatching and RNA synthesis by influenza polymerase Structure of influenza A polymerase bound to the viral RNA promoter Structural Insights into Bunyavirus Replication and Its Regulation by the vRNA Promoter A virus-like particle system identifies the endonuclease domain of Crimean-Congo hemorrhagic fever virus Biochemical and structural studies reveal differences and commonalities among cap-snatching endonucleases from segmented negative-strand RNA viruses Structure of a functional cap-binding domain in Rift Valley fever virus L protein Structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domaincontaining protease Crimean-Congo Hemorrhagic Fever Virus Suppresses Innate Immune Responses via a Ubiquitin and ISG15 Specific Protease Potent and selective inhibition of pathogenic viruses by engineered ubiquitin variants Stable Occupancy of the Crimean-Congo Hemorrhagic Fever Virus-Encoded Deubiquitinase Blocks Viral Infection Baculovirus expression system for heterologous multiprotein complexes MultiBac: multigene baculovirus-based eukaryotic protein complex production Recombinant RNA-Dependent RNA Polymerase Complex of Ebola Virus Mechanism of Inhibition of Ebola Virus RNA-Dependent RNA Polymerase by Remdesivir Structure-function relationships among RNA-dependent RNA polymerases The antiviral compound remdesivir potently inhibits RNA-dependent RNA polymerase from Middle East respiratory syndrome coronavirus RNA ligands activate the Machupo virus polymerase and guide promoter usage Molecular Basis for the Selective Inhibition of Respiratory Syncytial Virus RNA Polymerase by 2'-Fluoro-4'-Chloromethyl-Cytidine Triphosphate Hepatitis C virus RNA-dependent RNA polymerase (NS5B) as a mediator of the antiviral activity of ribavirin Deubiquitinase function of arterivirus papain-like protease 2 suppresses the innate immune response in infected host cells Assembly of a functional Machupo virus polymerase complex Towards a structural understanding of RNA synthesis by negative strand RNA viral polymerases Viral replication. Both drugs show a broad spectrum of activities against RNA viruses, including CCHFV


id: cord-352178-irjhmxsg
author: Saxton-Shaw, Kali D.
date: 2013-01-24
extension: .txt
mime: text/plain
words: 5960
sentence: 241
flesch: 44
cache: cache/cord-352178-irjhmxsg.txt
txt: txt/cord-352178-irjhmxsg.txt
title:
  O'nyong nyong Virus Molecular Determinants of Unique Vector Specificity Reside in Non-Structural Protein 3

summary:
  The use of chimeric Venezuelan equine encephalitis viruses as an approach for the molecular identification of natural virulence determinants Structural and nonstructural protein genome regions of eastern equine encephalitis virus are determinants of interferon sensitivity and murine virulence Vector infection determinants of Venezuelan equine encephalitis virus reside within the E2 envelope glycoprotein Determinants of vector specificity of o'nyong nyong and chikungunya viruses in Anopheles and Aedes mosquitoes Intracellular immunization of mosquito cells to LaCrosse virus using a recombinant Sindbis virus vector Manual for Mosquito Rearing and Experimental Techniques Virulence variation among isolates of western equine encephalitis virus in an outbred mouse model Lineage replacement accompanying duplication and rapid fixation of an RNA element in the nsP3 gene in a species of alphavirus Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences Evolutionary conservation of histone macroH2A subtypes and domains Functions of alphavirus nonstructural proteins in RNA replication Amino acid mutations in the replicase protein nsP3 of Semliki Forest virus cumulatively affect neurovirulence Deletion and duplication mutations in the C-terminal nonconserved region of Sindbis virus nsP3: effects on phosphorylation and on virus replication in vertebrate and invertebrate cells SH3 Domain-Mediated Recruitment of Host Cell Amphiphysins by Alphavirus nsP3 Promotes Viral RNA Replication Deletions in the hypervariable domain of the nsP3 gene attenuate Semliki Forest virus virulence In vitro synthesis of infectious venezuelan equine encephalitis virus RNA from a cDNA clone: analysis of a viable deletion mutant Semliki Forest virusspecific non-structural protein nsP3 is a phosphoprotein Phosphorylation of Sindbis virus nsP3 in vivo and in vitro Elimination of phosphorylation sites of Semliki Forest virus replicase protein nsP3 Phosphorylation site analysis of Semliki forest virus nonstructural protein 3 Deletion mapping of Sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging Mutagenesis of the 39 nontranslated region of Sindbis virus RNA The 39 untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cells We would like to thank Andrea Peterson for maintaining the An. gambiae, G3 colony used in this study. When ONNV non-structural protein 3 (nsP3) replaced nsP3 from CHIKV virus in one of the chimeric viruses, infection rates in An. gambiae went from 0% to 63.5%.


id: cord-296226-ugeupo3u
author: Sim, Shuzhen
title: A greener vision for vector control: The example of the Singapore dengue control programme
date: 2020-08-27
extension: .txt
mime: text/plain
words: 6877
sentence: 268
flesch: 40
cache: cache/cord-296226-ugeupo3u.txt
txt: txt/cord-296226-ugeupo3u.txt
summary:
  The World Health Organization calls for strengthening of vector control programmes in line with the Global Vector Control Response (GVCR) strategy, and many vector control programmes are transitioning to this new approach. Entomological indices and dengue case numbers are not reliable measures for assessing the long-term impact of vector control programmes because of changes in surveillance and diagnostic capabilities over time [30] .


id: cord-345494-8lcdx719
author: Chao, Chien-Chung
date: 2015-07-10
extension: .txt
mime: text/plain
words: 7093
sentence: 281
flesch: 49
cache: cache/cord-345494-8lcdx719.txt
txt: txt/cord-345494-8lcdx719.txt
title:
  Development of Recombinase Polymerase Amplification Assays for Detection of Orientia tsutsugamushi or Rickettsia typhi

summary:
  On the contrary, the difference in detection limit was less pronounced for R. typhi detection as shown in Table 2 . typhi DNA detection in RPA-nfo could not detect R. conorii and R. rickettsii DNA with similar detection limit.


id: cord-287582-ya81rc2n
author: Viennet, Elvina
date: 2016-09-19
extension: .txt
mime: text/plain
words: 9742
sentence: 465
flesch: 48
cache: cache/cord-287582-ya81rc2n.txt
txt: txt/cord-287582-ya81rc2n.txt
title:
  Public Health Responses to and Challenges for the Control of Dengue Transmission in High-Income Countries: Four Case Studies

summary:
  A robust assessment of the economic burden helps to i) identify information gaps, research needs, and refinements to the national statistical reporting systems; ii) argue that policies on dengue control and prevention should be given a high priority on the public health policy agenda; and iii) provide a baseline measure to determine the cost effectiveness of dengue policies and programs [144] ) from dengue-endemic countries and decreasing herd immunity have contributed significantly to the failure of dengue control in Singapore [145] .


id: cord-312223-qgwzgazd
author: Shafagati, Nazly
date: 2013-07-04
extension: .txt
mime: text/plain
words: 8854
sentence: 433
flesch: 55
cache: cache/cord-312223-qgwzgazd.txt
txt: txt/cord-312223-qgwzgazd.txt
title:
  The Use of NanoTrap Particles as a Sample Enrichment Method to Enhance the Detection of Rift Valley Fever Virus

summary:
  Viral inactivation was assayed by plaque assays (C) and viral RNA was extracted from the particles with Ambion's MagMax 96-well Viral RNA extraction kit and quantitated by qRT-PCR (D Comparison of capture efficacy of NanoTrap particles and commercially available beads for RVFV capture NanoTrap particles have unique properties not demonstrated in other beads that are used for protein purification and albumin exclusion such as dye baits that make them an ideal candidate in virus capture. Characterization of RVFV NanoTrap particle capture.


id: cord-352771-s0hfsxzb
author: Barriga, Gonzalo P.
date: 2016-07-14
extension: .txt
mime: text/plain
words: 8928
sentence: 325
flesch: 44
cache: cache/cord-352771-s0hfsxzb.txt
txt: txt/cord-352771-s0hfsxzb.txt
title:
  Inhibition of the Hantavirus Fusion Process by Predicted Domain III and Stem Peptides from Glycoprotein Gc

summary:
  (E) Cytotoxicity analysis of Gc DIII proteins and stem peptides on Vero E6 cells. Although targeting the endosomal site of virus fusion has not yet been optimized, it was possible to block fusion and infection under physiological virus entry conditions.


# ent

id: cord-007383-5yb3dxse
sid: 40
eid: 1
type: ORG
entity:
  the seoul national university hospital and international vaccine institute institutional animal care


