REINWARDTIA A JOURNAL ON TAXONOMIC BOTANY, PLANT SOCIOLOGY AND ECOLOGY Vol. 14(2): 249-324, December 23, 2015 Chief Editor Kartini Kramadibrata (Mycologist, Herbarium Bogoriense, Indonesia) Editors Dedy Darnaedi (Taxonomist, Herbarium Bogoriense, Indonesia) TukirinPartomihardjo (Ecologist, Herbarium Bogoriense, Indonesia) Joeni Setijo Rahajoe (Ecologist, Herbarium Bogoriense, Indonesia) Marlina Ardiyani (Taxonomist, Herbarium Bogoriense, Indonesia) Topik Hidayat (Taxonomist, Indonesia University of Education, Indonesia) Eizi Suzuki (Ecologist, Kagoshima University, Japan) Jun Wen (Taxonomist, Smithsonian Natural History Museum, USA) Managing Editor Himmah Rustiami (Taxonomist, Herbarium Bogoriense, Indonesia) Lulut Dwi Sulistyaningsih (Taxonomist, Herbarium Bogoriense, Indonesia) Secretary Endang Tri Utami Layout Medi Sutiyatno Illustrators Subari Wahyudi Santoso Anne Kusumawaty Correspondence on editorial matters and subscriptions for Reinwardtia should be addressed to: HERBARIUM BOGORIENSE, BOTANY DIVISION, RESEARCH CENTER FOR BIOLOGY-INDONESIAN INSTITUTE OF SCIENCES CIBINONG SCIENCE CENTER, JLN. RAYA JAKARTA - BOGOR KM 46, CIBINONG 16911, P.O. Box 25 CIBINONG INDONESIA PHONE (+62) 21 8765066; Fax (+62) 21 8765062 E-MAIL: reinwardtia@mail.lipi.go.id http://e-journal.biologi.lipi.go.id/index.php/reinwardtia A C B D E G F H Cover images: Zingiber engganoensis Ardiyani. A. Habit B. Leafy shoot and the inflorescence showing rhizomes, roots and root-tuber C. Leaves D. Ligule and swollen petiole E. Dissection of inflorescence showing fruit F. Spike and flowers G. Dissection of flowers and fruits showing bract, bracteole, two lateral staminodes, two petal lobes, labellum, and the four appendages of the anther H. Flower. Source of materials: E190 (BO). Photo credits: B, C, D by Arief Supnatna. A, E, F, G, H by Marlina Ardiyani. The Editors would like to thank all reviewers of volume 14(2): Abdul Latiff Mohamad, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Malaysia Abdulrokhman Kartonegoro - Herbarium Bogoriense, Bogor, Indonesia Agus Susatya - University of Bengkulu, Bengkulu, Indonesia Axel D. Poulsen - Royal Botanic Garden Edinburgh, Edinburgh, Scotland, UK Campbell O. Webb - Arnold Arboretum, University of Harvard, USA Edwino Fernando - Dept. of Forest Biological Sciences, University of the Philippines, Los Baños, Philippines Fabian Brambach - Dept. of Ecology & Ecosystem Research, Georg August University, Gottingen, Germany John Mood - Lyon Arboretum, University of Hawaii, USA Kuswata Kartawinata - Integrative Research Center, The Field Museum, Chicago, USA Mark Newman - Royal Botanic Garden Edinburgh, Edinburgh, Scotland, UK Martin Dancak - Faculty of Science, Palacky University, Czech Republic Mien A. Rifai - Akademi Ilmu Pengetahuan Indonesia (AIPI) Ridha Mahyuni - Herbarium Bogoriense, Bogor, Indonesia REINWARDTIA Vol 14, No 2, pp: 307 ‒ 310 307 numbers for the three barcoding regions are summa- rized in Table 2. SPECIES DESCRIPTION Zingiber engganoensis Ardiyani, spec. nov. Fig. 1, Plate 1. — Type: Indonesia, Bengkulu Province, Eng- gano Island, Jangkar River, 17 m, 5°21'37"S, 102° 16'57"E, 25 April 2015, M. Ardiyani, W. Wardani & D. Rosalina, Enggano190 (holo: BO). Zingiber engganoensis differs from Zingiber spectabile in its thin, and plicate leaves, glaucous leaf underside, structure of labellum which narrows towards the base, rounded and emarginate at the apex, and separated from the lateral staminodes. Leafy shoot to 5 m tall, not clumping, with ± 25 leaves; base light green, pubescent, 3 cm in diameter. Rhizome light brown externally, cream internally, with a few tubercles. Tubercle narrow elliptic, cream to light brown externally, cream internally. Leaf sheath covered with pubescent light red scales. Ligule bilobed and split to the base, apex acute-round, 0.5−1.7 × 1.2−1.3 cm, pubescent, light green, thin, papery in dried specimen. Petiole swollen, tilted to left or right 45o, light green, pubescent. Leaves linear-elliptic towards basal, linear-lanceolate towards terminal, 18.5 × 3.5 to 46 × 9.5 cm, pubescent, light green above, glaucous underside, base obtuse-round, oblique, apex acuminate-caudate (cauda 1−1.5 cm long), plicate (seen as thick lines in dried specimen), thin (papery in dried specimen). Inflorescence radical, attached INTRODUCTION In 1936, a Dutch botanist, Wilhelm Jan Lütjeharms did a botanical collection on Enggano Island (van Steenis, 1950), located ca. 100 km southwest of Bengkulu, Sumatra, Indonesia. Studying the geological history of this island revealed that it was never connected to Sumatra, hence its flora is most likely unique. Lütjeharms only recorded Etlingera hemisphaerica (Blume) R.M. Sm., but no Zingiber. In the intervening years, no further collections have been done on the Island. Recently, a group of researchers from Research Center for Biology, the Indonesian Institute of Sciences revisited Enggano Island. The expedition managed to collect one species of Zingiber which morphologically resembled Z. spectabile. Neverthe- less, close morphological examination reveal the taxon to be different from Z. spectabile. MATERIALS AND METHODS The morphology of the new species was described from living plants collected in the field. Detailed morphological measurements were made using ruler and a calibrated eye piece under a dissecting microscope. The holotype was obtained from the living plants growing in the field. DNA extraction, amplification and sequencing were carried out for three barcoding regions of rbcL and matK, and the nuclear Internal Trancribed Spacer 2 (ITS2). DNA amplification was performed using published primers under standard conditions (see Kress and Erickson, 2007). GenBank accession A NEW SPECIES OF ZINGIBER (ZINGIBERACEAE) FROM ENGGANO ISLAND, INDONESIA Received July 03, 2015; accepted September 09, 2015 MARLINA ARDIYANI Herbarium Bogoriense, Botany Division, Research Center for Biology-LIPI, Cibinong Science Center, Jln. Raya Jakarta-Bogor Km. 46, Cibinong 16911, Bogor, Indonesia. E-mail: marlina.ardiyani@lipi.go.id ABSTRACT ARDIYANI, M. 2015. — A new species of Zingiber (Zingiberaceae) from Enggano Island, Indonesia. Reinwardtia 14 (2): 307 ‒ 310. — A species of Zingiber Miller (Zingiberaceae), Z. engganoensis Ardiyani, from Enggano Island, Indo- nesia is described. The species is only known from its type locality. It is similar to Z. spectabile Griff. but with some morphological differences. Three-locus DNA barcodes (rbcL, matK and ITS2) of the new species were generated for its identification purposes. Key Words: DNA bar code, Enggano Island, Z ingiber, Zingiberaceae. ABSTRAK ARDIYANI, M. 2015. Satu jenis baru keluarga Jahe-jahean (Zingiberaceae) dari Pulau Enggano, Indonesia. Reinward- tia 14(2): 307 ‒ 310. — Satu jenis baru keluarga Jahe-jahean (Zingiberaceae), Zingiber engganoensis Ardiyani dari Pulau Enggano, Indonesia dipertelakan. Jenis baru ini diketahui dari lokasi tipe saja, menyerupai Zingiber spectabile Griff., namun memiliki beberapa perbedaan karakter morfologi. Tiga buah lokus DNA barcode (rbcL, matK dan ITS2) dari jenis baru ini telah disekuens untuk keperluan identifikasi jenis. Kata Kunci: DNA barcode, Pulau Enggano, Z ingiber, Zingiberaceae. REINWARDTIA Vol 14, No 2, pp: 307 � 310 307 numbers for the three barcoding regions are summa- rized in Table 2. SPECIES DESCRIPTION Zingiber engganoensis Ardiyani, spec. nov. Fig. 1, Plate 1. — Type: Indonesia, Bengkulu Province, Eng- gano Island, Jangkar River, 17 m, 5°21'37"S, 102° 16'57"E, 25 April 2015, M. Ardiyani, W. Wardani & D. Rosalina, Enggano190 (holo: BO). Zingiber engganoensis differs from Zingiber spectabile in its thin, and plicate leaves, glaucous leaf underside, structure of labellum which narrows towards the base, rounded and emarginate at the apex, and separated from the lateral staminodes. Leafy shoot to 5 m tall, not clumping, with ± 25 leaves; base light green, pubescent, 3 cm in diameter. Rhizome light brown externally, cream internally, with a few tubercles. Tubercle narrow elliptic, cream to light brown externally, cream internally. Leaf sheath covered with pubescent light red scales. Ligule bilobed and split to the base, apex acute-round, 0.5−1.7 × 1.2−1.3 cm, pubescent, light green, thin, papery in dried specimen. Petiole swollen, tilted to left or right 45o, light green, pubescent. Leaves linear-elliptic towards basal, linear-lanceolate towards terminal, 18.5 × 3.5 to 46 × 9.5 cm, pubescent, light green above, glaucous underside, base obtuse-round, oblique, apex acuminate-caudate (cauda 1−1.5 cm long), plicate (seen as thick lines in dried specimen), thin (papery in dried specimen). Inflorescence radical, attached INTRODUCTION In 1936, a Dutch botanist, Wilhelm Jan Lütjeharms did a botanical collection on Enggano Island (van Steenis, 1950), located ca. 100 km southwest of Bengkulu, Sumatra, Indonesia. Studying the geological history of this island revealed that it was never connected to Sumatra, hence its flora is most likely unique. Lütjeharms only recorded Etlingera hemisphaerica (Blume) R.M. Sm., but no Zingiber. In the intervening years, no further collections have been done on the Island. Recently, a group of researchers from Research Center for Biology, the Indonesian Institute of Sciences revisited Enggano Island. The expedition managed to collect one species of Zingiber which morphologically resembled Z. spectabile. Neverthe- less, close morphological examination reveal the taxon to be different from Z. spectabile. MATERIALS AND METHODS The morphology of the new species was described from living plants collected in the field. Detailed morphological measurements were made using ruler and a calibrated eye piece under a dissecting microscope. The holotype was obtained from the living plants growing in the field. DNA extraction, amplification and sequencing were carried out for three barcoding regions of rbcL and matK, and the nuclear Internal Trancribed Spacer 2 (ITS2). DNA amplification was performed using published primers under standard conditions (see Kress and Erickson, 2007). GenBank accession A NEW SPECIES OF ZINGIBE R (ZINGIBERACEAE) FROM ENGGANO ISLAND, INDONESIA Received July 03, 2015; accepted September 09, 2015 MARLINA ARDIYANI Herbarium Bogoriense, Botany Division, Research Center for Biology-LIPI, Cibinong Science Center, Jln. Raya Jakarta-Bogor Km. 46, Cibinong 16911, Bogor, Indonesia. E-mail: marlina.ardiyani@lipi.go.id ABSTRACT ARDIYANI, M. 2015. — A new species of Zingiber (Zingiberaceae) from Enggano Island, Indonesia. Reinwardtia 14 (2): 307 � 310. — A species of Zingiber Miller (Zingiberaceae), Z. engganoensis Ardiyani, from Enggano Island, Indo- nesia is described. The species is only known from its type locality. It is similar to Z. spectabile Griff. but with some morphological differences. Three-loci DNA barcodes (rbcL, mat K and ITS2) of the new species were generated for its identification purposes. Key Words: DNA bar code, Enggano Island, Z ingiber, Zingiberaceae. ABSTRAK ARDIYANI, M. 2015. Satu jenis baru keluarga Jahe-jahean (Zingiberaceae) dari Pulau Enggano, Indonesia. Reinward- tia 14(2): 307 � 310. — Satu jenis baru keluarga Jahe-jahean (Zingiberaceae), Zingiber engganoensis Ardiyani dari Pulau Enggano, Indonesia dipertelakan. Jenis baru ini diketahui dari lokasi tipe saja, menyerupai Zingiber spectabile Griff., namun memiliki beberapa perbedaan karakter morfologi. Tiga buah lokus DNA barcode ( rbcL, matK dan ITS2) dari jenis baru ini telah disekuens untuk keperluan identifikasi jenis. Kata Kunci: DNA barcode, Pulau Enggano, Z ingiber, Zingiberaceae. REINWARDTIA 308 [VOL.14 to the base of the leafy shoot, erect, 40.5−49.5 cm long. Scape covered by up to 10 pubescent scales, 35−40 cm long, 2−2.5 cm diameter, pubescent, light green; scales overlapped but free, getting broader towards terminal, 2−5 cm long, light red, with dark brown longitudinal stripes in dried specimen. Spike cylindrical and tapering towards terminal, 13.5−18 × 4−4.5 cm, composed of overlapped, spirally arranged bracts. Bracts obovate with truncate apex, 2.9−3.5 × 3.1−3.5 cm, pubescent, bright red, getting white towards the base, with yellow-brown lines and black-dotted edge in spirit material, apex curled inward (curly part 2−2.5 mm long). Bracteoles cylindrical, split to the base, apex acute, cucullate, 3.6−4 × 0.5 cm, red, getting white towards the base, 3−5 mm top part protruding beyond the edge of the bracts. Calyx teethed, deeply split in one side, 1.6−1.7 × 1.3 cm, white transparent. Corolla total length 5.7 cm, cream white with reddish-brown tinge, with black-dotted edge and black lines in spirit material; dorsal lobe boat-shaped, elliptic, apex acute, slightly cucullate, 2.4−2.7 × 1 cm; lateral staminodes lobes linear-lanceolate, 2.3−2.4 × 5−6 mm. Labellum narrow at the base, getting broader towards the emarginate apex, 2.2 × 1.1 cm, reddish-brown with cream blotch; side lobes lanceolate, deeply incised, 14−16 × 4−5 mm, reddish-brown with cream dots. A nther bent ± 50o, 14 × 4 mm, 2 mm thick, yellow. A nther crest with S-shaped tip, 1.4 cm long, yellow. Stigma cup-shaped with fimbriate edge, white transparent. Ovary 5 × 4.5 mm, cream. Stylodes 2, subulate with acute apex, 9 × 1 mm, white. Fruit round, with cream-white calyx remnant, 1.6 × 1 cm, red, getting white towards the base. Locule contains 4 seeds, 1.4 × 7 mm. Seed oval, 8 × 4.5 mm, black, covered with white aril. Distribution. At pr esent this species is known only from the type locality. Habitat. Gr ows in secondar y for est along the steep river bank on thin soil over coral. Etymology. The specific epithet is der ived fr om the locality of the type, Enggano Island. Phenology. Zingiber engganoensis was observed in flower and fruit when it was collected at the end of April 2015. Conservation status. Not assessed. Notes. This species from Enggano Island resem- bles Z. spectabile especially in its inflorescence and red curved inward apex of the bracts. How- ever, the habit is actually different as the Enggano species was found almost solitaire separated from other plant. Close morphological observation re- vealed that the species from Enggano Island have some differences from Z. spectabile, tabulated in Table 1. ACKNOWLEDGEMENTS I would like to thank Kedeputian Bidang Ilmu Penge- tahuan Hayati (IPH), and Herbarium Bogoriense, Re- search Center for Biology, the Indonesian Institute of Sciences who let me join the Enggano Expedition. I am also indebted to Dr Ary Prihardhyanto Keim, Mr Tri, Mr Trisno Utomo, Mrs Wita Wardani, Dr Ruliyana Su- santi, Mrs Dewi Rosalina and my other colleagues in the Enggano expedition who helped me in the field and in collecting the specimens. I would also like to thank to Dr John Mood for precious discussion. My thanks are also due to Susila in the Molecular Systematics Lab, Mr Subari, Mrs Anne and Mr Wahyu who helped and taught me to make the line drawing. Last but not least, I would like to thank the Editors and the Reviewers who helped me to improve the quality of this paper. REFERENCES KRESS, W.J. & ERICKSON, D.L. 2007. A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-Coding trnH-psbA spacer region. PLoS ONE 2: e508. VAN STEENIS, C.G.G.J. 1950. Flora Malesiana. Vol. I. Noordhoff, Jakarta, 639 p. Table 1. Comparison of morphological characters of Z. engganoenis Ardiyani and Z. spectabile Z. engganoensis Ardiyani Z. spectabile Habit Non-clumping Clumping Ligule Petiole Bilobed, split to the base with rounded apex Subsessile, swollen, tilted to left or right 45o Entire Subsessile, slightly swollen, slightly tilted Leaf colour Leaf coating Light green with no clear greenish-white mid- rib above Glaucous underside Dark green with clear greenish-white midrib above Glaucous Leaf shape Linear-elliptic to linear-lanceolate with obtuse- round base and acuminate-caudate apex Linear-lanceolate with obtuse-round base and acuminate apex Leaf surface Plicate, thin Non-plicate, thick Flower labellum Narrow at the base with rounded and emar- ginate apex Broad at the base, getting narrow towards the apex with deeply incised side lobes Lateral staminodes Separated from labellum Joined with the labellum 2015] 309 ARDIYANI: A new species of Zingiber from Enggano Fig. 1. Line drawing of Zingiber engganoensis Ardiyani. A. Leaves B. Swollen petiole and split ligule C. Leafy shoot, inflorescence peduncle and root system D. Inflorescence E. A single flower sits on the bract F. Bract and brac- teole G. Whole flower H. Flower with labellum and lateral staminodes removed I. Calyx J. Calyx spread up K. Ovary longitudinal section with stylodes L. Ovary cross section M & N. Anther and appendage O. S-shaped appendage and pistillum P. Fruit Q. Locule containing seeds R. Individual seed covered with arils. (Line draw- ing by Marlina Ardiyani). Table 2. Voucher information and Genbank accession numbers for Zingiber engganoensis Species Gene region Genbank accession number Voucher (Herbarium location) Zingiber engganoensis rbcL KT026199 Enggano190 (BO) matK KT026200 Enggano190 (BO) ITS2 KT026201 Enggano190 (BO) REINWARDTIA 310 [VOL.14 Plate 1. Zingiber engganoensis Ardiyani. A. Habit B. Leafy shoot and the inflorescence showing rhizomes, roots and root-tuber C. Leaves D. Ligule and swollen petiole E. Dissection of inflorescence showing fruit F. Spike and flowers G. Dissection of flowers and fruits showing showing bract, bracteole, two lateral staminodes, two petal lobes, labellum, and the four appendages of the anther H. Flower. Source of materials: E190 (BO). Photo credits: B, C, D by Arief Supriatna. A, E, F, G, H by Marlina Ardiyani. INSTRUCTION TO AUTHORS Scope. R einwardtia is a scientific ir r egular jour nal on plant taxonomy, plant ecology and ethnobotany published in December. Manuscript intended for a publication should be written in English. Titles. Titles should be br ief, infor mative and followed by author ’s name and mailing address in one- paragraphed. Abstract. English abstr act followed by Indonesian abstr act of not mor e than 250 wor ds. Keywor ds should be given below each abstract. Manuscript. Manuscr ipt is or iginal paper and r epr esent an ar ticle which has not been published in any other journal or proceedings. The manuscript of no more than 36 pages by using Times New Roman 11, MS Word for Windows of A4 with double spacing, submitted to the editor through . New paragraph should be indented in by 5 characters. For the style of presentation, authors should follow the latest issue of Reinwardtia very closely. Author(s) should send the preferred running title of the article submitted. Every manuscript will be sent to two blind reviewers. Identification key. Taxonomic identification key should be pr epar ed using the aligned couplet type. Nomenclature. Str ict adher ence to the Inter national Code of Botanical Nomenclatur e is obser ved, so that taxonomic and nomenclatural novelties should be clearly shown. English description for new taxon proposed should be provided and the herbaria where the type specimens area deposited should be presented. Name of taxon in taxonomic treatment should be presented in the long form that is name of taxon, author’s name, year of publication, abbreviated journal or book title, volume, number and page. Map/line drawing illustration/photograph. Map, line dr awing illustr ation, or photogr aph pr efer ably should be prepared in landscape presentation to occupy two columns. Illustration must be submitted as original art accompanying, but separated from the manuscript. The illustration should be saved in JPG or GIF format at least 350 pixels. Legends or illustration must be submitted separately at the end of the manuscript. References. Bibliogr aphy, list of liter atur e cited or r efer ences follow the Har var d system as the following examples. Journal : KRAENZLIN, F. 1913. Cyrtandraceae novae Philippinenses I. Philipp. J. Sci. 8: 163–179. MAYER, V., MOLLER, M., PERRET, M. & WEBER, A. 2003. Phylogenetic position and generic differentiation of Epithemateae (Gesneriaceae) inferred from plastid DNA sequence data. American J. Bot. 90: 321–329. Proceedings :TEMU, S. T. 1995. Peranan tumbuhan dan ternak dalam upacara adat “Djoka Dju” pada suku Lio, Ende, Flores, Nusa Tenggara Timur. In: NASUTION, E. (Ed.). Prosiding Seminar dan Lokakarya Nasional Etnobotani II. LIPI & Perpustakaan Nasional: 263–268. (In Indonesian). SIMBOLON, H. & MIRMANTO, E. 2000. Checklist of plant species in the peat swamp forests of Central Kalimantan, Indonesia. In: IWAKUMA, T. et al. (Eds.) Proceedings of the International Symposium on: Tropical Peatlands. Pp.179-190. Book : RIDLEY, H. N. 1923. Flora of the Malay Peninsula 2. L. Reeve & Co. Ltd, London. Part of Book : BENTHAM, G. 1876. Gesneriaceae. In: BENTHAM, G. & HOOKER, J. D. Genera plantarum 2. Lovell Reeve & Co., London. Pp. 990–1025. Thesis : BAIRD, L. 2002. A Grammar of Kéo: An Austronesian language of East Nusantara. 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