REINWARDTIA Vol. 10, Part 4 , 4 1 9 - 4 2 3 (1988) THE JAVANESE SPECIES OF TETRAPLOA MIEN A. RIFAI "Herbarium Bogoriense", Puslitbang Biologi — LIPl, Bogor HARDANIAH ZAINUDDIN, Faculty of Public Health, Hasanuddin University, Ujung Pandang & ABDUL CHOLIL Faculty of Agriculture, Brawidjaja University, Making ABSTRACT Two specieg of the hyphomycete genus Tetraploa are reported from Java, namely the widespread Tetraploa aristata and the newly described Tetraploa javanica. Illustrated descriptions are presented for both species. ABSTRAK Dua jenis jamur Tetraploa dilaporkan dari Jawa, yaitu Tetraploa aristata dan jenis baru Tetraploa javanica. Pertelaan bergambar untuk masing-masing jenis disajikan. In his revision of Tetraploa Berk. & Br., Ellis (1949) considered that five species could be recognized in this hyphomycete genus. Only two of these are widely distributed, namely the cosmopolitan Tetraploa aristata Berk. & Br. and the less common Tetraploa ellisii Cooke (Ellis 1971). As might be expected the former species occurs also in Java, whereas the fatter so far is known only from USA, Argentina and Rhodesia but more recently Yokoyama & Tubaki (1973) reported its occurence in Papua New Guinea. A species which appears to be undescribed has been collected in Bogor Botanic Gardens. Since under a casual examination this new species can be easily mistaken for Tetraploa aristata, for comparative purposes we take this opportunity to give a detailed account of the two species as components of the mycoflora of Java. TETRAPLOA Berk. & Br. Tetraploa Berk. & Br. in Ann. Mag. nat. Hist. II, 5 : 459.1850; Sacc., Syll, Fung. 4 : 516. 1886; Clem. & Shear, Gen. Fung. : 217. 1931; Barnet, III Gen. Imperf. Fung. : 130. 1960; Ellis, Demat. Hyphomyc. : 51. 1971; Carmichael et al., Gen. Hyphomyc. : 184. 1980. Type species : Tetraploa aristata Berk. & Br. 410 REINWARDTIA KEY TO JAVANESE TETRAPLOA 1. a. Conidia obovoid in outline, mostly with 4 cells in each column, setiform appendages divaricate, more or less of equal length Tetraploa aristata b. Conidia ovoid in outline, mostly with 6 cells in each column, setiform appendages almost fascicled, strongly unequal in length, two long and two very short Tetraploa javanica TETRAPLOA ARISTATA Berk. & Br. — Fig. 1. Tetraploa aristata Berk. & Br. in Ann. Mag. nat. Hist. II, 5 : 459. 1850; Ellis in Trans. Brit, mycol. Soc. 32 : 249. 1949; Demat Hyphomyc. : 52. 1971; Yokoyama & Tubaki in Bull. Nat. Sc. Mus. 16 : 658. pi. 5 D - E. 1973. Colonies effused, greyish brown. Mycelium superficial, consisted of pale brown, smooth walled, flexuous, septate, much branched an.d anastomosing hyphae 1.8 — 3.5 yum diam. which form a compact network. Conidiophores micronematous, hardly distinguishable from the mycelium, with integrated, short cylindrical, intercalary, determinate and monoblastic eonidiogenous cells. Conidia solitary, obovoid to oblong obovoid in outline, 25 — 40 x 14 — 28µm, brown, wall distincly verrucose, consist of 4 columns or rows of 9 — 16 µm diam. cells and mostly with 4 cells to each row which tends to diverge from one another apically and terminated by an almost straight, septate, setiform appendage which measures 10 — 90 µm long, up to 8µm diam. at the base and taper into its 2 — 3.5 µm paler apex; these four appendages therefore divaricate and generally more or less of equal length. SPECIMENS EXAMINED : on culm of a decaying tall grass (? Saccharum), Bogor Botanic Gardens, Java, December 1930, KB. Boedijn 865 (BO). Tetraploa javanica spec. nov. — Fig. 2 Coloniae effusae, velutinosae, atrobrunneae. Mycelium superficiale. Conidiopbora micronematica, ex hyphis septatis, irregulariter ramosis, anas- tomosis, flexuosis, laevis, 1.6 — 4µm diam. composita. Cellulae conidiogenae integratae, monoblasticae. Conidia ovoidea, brunnea, laevia, 30 — 42 (— 58) x 17 — 28µm, plerumque ex 4- seriebus cellularibus (unaquaeque series 4 — 7 cellulatis 8 — 18µm diam.) composita, apice in appendicum longum (— 215µm) et appendicum brevem (— 25µm) transmutata. KaD. in culmis emortois Bambusae glaucescentis, Hortus Botanicus Bogor, Java, Januarii 1988, Rifai 7920 typus est (BO). Colonies effuied , velvety, dark brown to blackish brown. Mycelium superficial, composed of pale brown to brown, smooth walled, flexuous, septate, irregularly branched and anastomosing 1.6 — 4µm diam. hyphae which form a compact network on the surface of the substrate. Conidio- phores micronematous, hardly distinguishable from the mycelium, consisted of integrated, determinate, intercalary, short cylindrical, monoblastic or 1988] RIFAI et al : Javanese Tetraploa. 4 2 1 Fig. 1. Tetraploa aristata: conidia 422 REINWARDTIA [VOL. 10 Fig. 2. Tetraploa javanica: mycelium and conidia. 1.9881 RIFAI et al. : Javanese Tetraploa. 423 polyblastic conidiogenous cells. Conidia solitary, pleurogenous, as a rule ovoid in outline, 30 — 42 (— 58) x 17 — 2 8 µ m , smooth walled (or at the most the wall faintly verrucose), consisted of 4 (or rarely up to 6) rows or columns of brown cells, mostly with 4 — 7 cells measuring 8 — 1 8 µ m diam. to each column which tends to appear fascicled apically with each other and topped by appendage of unequal lengun. ueneraily in each conidium there are two long setiform appendages which are originating from opposing columns, almost straight, up to 215µm long and gently attennuate from the pale brown 5.4 — 8µm diam. base to about 2.5 — 3.5µm at the much paler, thinner walled apex, elongate by proliferations, only slightly diverge from the long axis of the conidium body; from the remaining columns emerge two short appendages measuring 9 — 2 5 µ m long which recurve sideway almost at right angle to the long axis of the conidium body. SPECIMENS EXAMIND : on culms of decaying Bambusa glaucescens, Bogor Botanic Garden, Java, March 1980, A. Cholil & Hardaniah Zainuddin (BO); 31 December 1980, Hardaniah Zainuddin & M.A. Rifai s.n. (BO); January 1988, M.A. Rifai 7920 (BO, holotype); on dead Bambusa glaucescens hedge, Bogor, Java, February 1988, E.A. Widjaja, Rugayah & M.A. Rifai s.n. (BO). It should be noted that the substrate of this new species, Bambusa glaucescens, is not native to Java but introduced from Japan. It would be of interest to search for this species in the native country of this bamboo species. Tetraploa javanica can be easily distinguished from the other species of Tetraploa by its ovoid conidia and the seemingly fascicled dimorphic appendages. REFERENCES ELLIS, M.B. 1949. Tetraploa. In Trans. Brit, mycol. Soc. 32 : 246 — 257. ELLIS, M.B. 1971. Dematiaceous Hyphomycetes. CMI, Kew. 608 pp. YOKOYAMA, T. & TUBAKI, K. 1973. Some Hyphomycetes from Papua and New Guinea. In Bull. Nat. Sc. Mus. 16 : 655 — 660. 10(4) 419_Page_1 Binder cover-100_Page_013