LIPI A JOURNAL ON TAXONOMIC BOTANY, PLANT SOCIOLOGY AND ECOLOGY 12(4) REINWARDTIA A JOURNAL ON TAXONOMIC BOTANY, PLANT SOCIOLOGY AND ECOLOGY Vol. 12(4): 261 - 337, 31 March 2008 Editors ELIZABETH A. WIDJAJA, MIEN A. RIFAI, SOEDARSONO RISWAN, JOHANIS P. MOGEA Correspondece on The Reinwardtia journal and subscriptions should be addressed to HERBARIUM BOGORIENSE, BIDANG BOTANI, PUSAT PENELITIAN BIOLOGI - LIPI, BOGOR, INDONESIA THE PHYLOGENETIC POSITION OF THE PAPUASIAN GENUS SARCOCHILUS R.BR. (ORCHIDACEAE: AERIDINAE): EVIDENCE FROM MOLECULAR DATA Received August 8, 2007; accepted October 28, 2007. TOPIK HIDAYAT Department of Biology Education, Faculty of Mathematic and Natural Science Education, Indonesia University of Education (UPI), Bandung, Indonesia. topikhidayat@upi.edu MOTOMI ITO Department of General Systems Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan TOMOHISA YUKAWA Tsukuba Botanical Garden, National Science Museum, Tsukuba, Japan ABSTRACT HIDAYAT, T.; ITO, M.; YUKAWA, T. 2008. The phylogenetic position of the Papuasian genus Sarcochilus R.Br. (Orchidaceae: Aeridinae): evidence from molecular data. Reinwardtia 12(4). 281 – 284. –– The taxonomic status of the Papuasian orchid genus Sarcochilus R.Br. remains unresolved. Represented by Sarcochilus chrysanthus Schltr., a phylogenetic analysis to evaluate relationships between the Papuasian species and those from Australia was conducted using molecular characters. Parsimony analysis using DNA sequences of the internal transcribed spacer (ITS) region showed that this Papuasian species, is in a distant position from the so-called the true Sarcochilus sensu stricto. These results provide additional evidence for the establishment of a new genus Monantochilus. Keywords: ITS region, Orchidaceae, Papua New Guinea, phylogenetic relationships, Sarcochilus chrysanthus ABSTRAK HIDAYAT, T.; ITO, M.; YUKAWA, T. 2008. Posisi filogenetika anggrek Papua marga Sarcochilus (Orchidaceae: Aeridinae): bukti dari data molekuler. Reinwardtia 12(4): 281 – 284. –– Status taksonomi marga anggrek Sarcochilus yang berasal dari Papua masih belum terselesaikan. Dengan menggunakan Sarcochilus chrysanthus Schltr., analisis filogenetik untuk mengevaluasi hubungan antara jenis yang berasal dari Papua. dengan jenis yang berasal dari Australia telah dilakukan menggunakan ciri molekul. Analisis parsimoni dengan menggunakan urutan DNA dari daerah internal transcribed spacer (ITS) menunjukkan bahwa jenis yang berasal dari Papua ini sangat berbeda dengan jenis Sarcochilus yang sebenarnya. Hasil ini menyediakan bukti tambahan bagi pembentukan marga baru, yaitu Monantochilus. Kata kunci: Daerah ITS, hubungan filogenetik, Orchidaceae, Papua, Sarcochilus chrysanthus INTRODUCTION The orchid genus Sarcochilus R. Br., belongs to the vandaceous subtribe Aeridinae Pfitzer, which contains numerous poorly delimited genera providing many taxonomic and phylogenetic problems. Sarcochilus is primarily distributed in Australia, with only a few species occurring in neighbouring Papua New Guinea. The relationship between the Papuasian species of Sarcochilus and those of Sarcochilus sensu stricto from mainland Australia has long remained unanswered. Rice (2004) separated the Papuasian members of Sarcochilus from the Sarcochilus sensu stricto, placing them into a new genus Monantochilus (Schltr.) R. Rice. This separation was based upon habit, flower number, labellum mobility, and the existence of a callus in the spur. However, these characters vary greatly throughout the species. Given the shortcomings of these characters, data obtained from nucleotide substitutions of appropriate molecules are preferable in clarifying the phylogenetic relationships of this group (e.g., Moritz and Hillis 1996). REINWARDTIA Vol 12, Part 4, pp: 281 - 284 281 This study aims to evaluate the phylogenetic relationships between Papuasian Sarcochilus and Sarcochilus sensu stricto at molecular level using DNA sequences of the internal transcribed spacer (ITS) region. The ITS region has been widely used by plant systematists because of its small size, highly conserved flanks, high copy number, and rapid concerted evolution (Baldwin et al. 1995). Recently, the ITS region has been used in the phylogenetic analysis in many groups of Orchidaceae and has provided resolutions for previously obscure relationships. MATERIALS AND METHODS Six species of Sarcochilus, viz. the Papuasian S. chrysanthus and five from Australia, were examined in this study. Rhinerrhiza was used as an outgroup, since this genus is recognized as the sister group to Sarcochilus based upon morphological and macromolecular characters (Topik et al. 2005). Specimens were primarily collected from various regions of Australia, and another obtained from Tsukuba Botanical Garden, Japan. Detailed information about plant materials is provided in Table 1. Table 1. Plant materials examined in this study Notes: AU : Specimens were collected in Australia TBG : Specimens were collected Tsukuba Botanical Garden-Japan (TBG). NA : Not Available The total DNA was extracted from fresh material or silica-gel dried plant tissue with a QIAGEN DNeasy Mini Plant Kit following the manufacturer ’s instructions. Amplification, purification, and sequencing of ITS region were carried out as described in Topik et al. (2005) using a primer pair, 17SE and 26SE (Fig.1; Sun et al. 1994). Nuclear ribosomal DNA 17SE= ACGAATTCATGGTCCGGTGAAGTGTTCG 26SE= GAATTCCCCGGTTCGCTCGCCGTTAC Fig. 1. Sequence of ITS region with location of primers used in this study. Information on the primers is also provided. The DNA sequence obtained from the ITS region was aligned with Clustal X and was then adjusted manually following the guidelines described in Kelchner (2000). Phylogenetic analysis based on the maximum parsimony criterion was performed using PAUP* version 4.0b10 (Swofford 1998). Insertions and deletions were treated as missing data. All characters were equally weighted and unordered (Fitch 1971). Data were analyzed by the heuristic search method with tree bisection- reconnection (TBR) branch swapping and the MULTREES option on, ten replications of random addition sequences with the stepwise addition option, and all most parsimonious trees (MPTs) were saved. Evaluation of internal support of clades was conducted by the bootstrap analysis (Felsenstein 1985) utilizing 1,000 replicates with TBR branch swapping and the MULTREES option off. Number of steps, consistency indices (CI) and retention indices (RI) were calculated on one of the MPTs in each analysis with the TREE SCORES command in PAUP*. RESULTS The aligned ITS region comprised 662 characters. Of these, 547 (83%) were constant and 32 (4.8%) were potentially informative. The analysis resulted in one MPT with the length of 153 steps, CI (excluding autapomorphies) of 0.876 and RI of 0.548. The strict consensus tree (Fig. 2) showed that Papuasian Sarcochilus, in this study represented by S. chrysanthus, is separated from Sarcochilus sensu stricto. Moreover, monophyly of Sarcochilus from Australia is clearly defined, although bootstrap 282 REINWARDTIA [VOL.12 Taxon Source Voucher Rhinerrhiza divitiflora (Benth.) Rupp Sarcochilus hartmannii F. Mueller Sarcochilus hirticalcar (Dockrill) M.A. Clem. & B.J. Wallace Sarcochilus moorei Schltr. Sarcochilus spathulatus R.S. Rogers Sarcochilus chrysanthus Schltr. Sarcochilus weinthalii F.M. Bailey AU TBG AU AU AU TBG AU NA TBG145793 NA NA NA TBG145831 NA ITS region 17SE 26 SE NTS ETS 18S ITS-1 5-8S ITS-2 26S Fig. 2. Strict consensus tree derived from the parsimony analysis of the ITS region of nrDNA. Bootstrap percentages of > 50 are shown above each branch. DISCUSSION From global phylogenetic analyses on Aeridinae Regarding the phylogenetic position of the genus Sarcochilus, a recent global phylogenetic analysis of subtribe Aeridinae based on DNA (ITS and matK sequences) contributes some information (Topik et al. 2005). In that analysis, Sarcochilus is represented by S.chrysanthus and S. hartmannii. On this limited basis, Sarcochilus appears to be non- monophyletic. The former makes up Thrixspermum alliance with Thrixspermum, Dimorphorchis, Abdominea, Microsaccus, and Cleisomeria, whereas the latter makes monophyletic with Rhinerrhiza and Bogoria. Using much greater taxon sampling of Sarcochilus more robust phylogenetic hypotheses for the genus at molecular level could be established, as can be seen in this study Generic circumscription As mentioned above that, at the molecular level, Papuasian Sarcochilus is different from Sarcochilus sensu stricto. In this case, the consequences were whether to align the Papuasian species into the Australian ones, or to split the former into a new genus. The latter option seemed to be the most practical choice, to avoid Sarcochilus in becoming non-monophyletic. Schlechter (1913) defined Sarcochilus rather broadly subdividing it into three sections based mainly upon stem length, the number of flowers on the sessile inflorescences, and lip lobe number; Schlechter ’s sections are Monantochilus, Eu- Sarcochilus, and Ascochilus. Rice (2004), however, circumscribed Sarcochilus in a narrower sense, including only species with a short leafy stem and many-flowered inflorescences. These two morphological characters are synapomorphic for the genus (Fig. 2). The result of this analysis demonstrates wide phylogenetic separation between S. chrysanthus and the Australian species of Sarcochilus. In comparison with Australian Sarcochilus (section Eu-Sarcochilus), the Papuasian S. chrysanthus has more elongated stems and spurs, and a single-flowered inflorescence. In addition, the presence of a rectangular, flattened callus in a distinct spur, and the absence of a column-foot are distinctive. The raising of section Monantochilus to a generic rank by Rice (2004) is supported b y this study at the molecular level. Two additional Papuasian species, S. iboensis and S. unifloris, have been transferred to Monantochilus by Rice (2004). Biogeographic overview In the past, Papua. was connected with Australia before finally it was separated as today (White 1990). Due to its strategic position, Papua since long time has been acting as a corridor for many groups of plant to migrate from Asia to Australia, and vice versa. Such pattern of migration has been suggested for Nothofagus (Setoguchi et al. 1997), Araucaria (Setoguchi et al. 1999), Amaryllidaceae (Ito et al. 1999), Dendrobium (Yukawa et al. 2000), and Aeridinae (Topik, 2005). We assumed that several members of Sarcochilus have separated at the time of the separation of Papua from Australia. Consequently, those Papuan species gradually have to shift their morphological features to fit with new conditions. It is natural that migration to a new region may be accompanied by several morphological changes to adapt to new environmental conditions before subsequently they diverge (White 1990). ACKNOWLEDGMENTS The authors gratefully acknowledge Peter H. Weston, Rod Rice and Mike Harrison for providing most of the materials used in this study. This study was partly supported by a Grant-in-Aid for Scientific Research from JSPS (MI). Jeffrey Wood of the Royal Botanic Gardens, Kew and Kunio Iwatsuki of The University of the Air, Japan kindly read through the manuscript and also Mien A. Rifai who kindly did correction of the language. S. hartmannii S. spathulatus S. hirticalcar S. moorei S. weinthalii S. chrysanthus Rhinerrhiza divitiflora Genus Sarcochilus 2008] HIDAYAT et al:: The phylogenetic position of Sarcochilus 283 66 99 99 percentages are relatively low (only 66). 284 REINWARDTIA [VOL.12 REFERENCES BALDWIN, B.G., SANDERSON, M.J., PORTER, J.M., WOJCIECHOWSKI, M.F., CAMPBELL, C.S, DONOGHUE, M.J. 1995. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on Angiosperm phylogeny. Ann. Missouri Bot. Gard. 82:247-277. FELSENSTEIN, J. 1985. Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783-791. FITCH, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20:406-416. 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In: Wilson KL, Morrison DA [Eds.] Monocots: Systematic and Evolution, CSIRO publishing, Melbourne, Australia, pp 465-471. INSTRUCTION TO AUTHORS Manuscripts intended for publication in Reinwardtia should be written either in English, French or German, and represent articles wich have not been published in any other journal or proceedings. Each manuscript received will be considered and processes further if it is accompanied by signed statements given independently by two reviewers chosen by the author (s) attestingto its merits as well as its scientific suitability for publication in Reinwardtia. Two printed copies (on A4 paper) of the manuscript of not more than 200 pages should be sent to Editors, together with an electronic copy prepared on Word Processor computer programme using Times New Romance letter type and saved as Rich Text File must be submitted. For the style of presentation autohrs should follow the latest issue of Reinwardtia very closely. 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The phylogenetic position of the Papuasian genus Sarcochilus R.Br. (Orchidaceae: Aeridinae): evidence frommolecular data 281 C.E. RIDSDALE. Notes on MaiesiznNeonaucleea 285 C.E. RIDSDALE. Thorny problems in the Rubiaceae: Benkara, Fagerlindia andOxyceros 289 KUSWATAKARTAWINAIA, PURWANINGSIH, T. PARTOMIHARDJO, R. YUSUF, R. ABDULHADI, S. RISWAN. Floristics and structure of a lowland dipterocarp forest at Wanariset Samboja, East Kalimantan, Indonesia 301 RUGAiAH & S. SUNARTI. Two new wild species of Averrhoa (Oxalidaceae) from Indonesia 325 ATIKRETNOWATI. Anew Javanese species of Marasmius (Trichlomataceae ) 334 Reinwardtia is a LEPI acredited Journal (80/Akred-LIPI/P2MBI/5/2007) HERBARIUM BOGORIENSE BIDANG BOTANI , PUS AT PENELITIAN BIOLOGI - LIPI BOGOR, INDONESIA depannnn 55-114-1-SM blkngg