id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cord-325479-2r4oomdp	Torii, Shotaro	Applicability of polyethylene glycol precipitation followed by acid guanidinium thiocyanate-phenol-chloroform extraction for the detection of SARS-CoV-2 RNA from municipal wastewater	2020-10-17		.txt	text/plain	5906	218	47	Katayama Hiroyuki: Resources, Project administration, Funding Acquisition, Supervision, Writing -review & editing First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus The MIQE Guidelines : Minimum Information for Publication of Quantitative Real-Time PCR Experiments Inactivation of an Enveloped Surrogate Virus in Human Sewage Evaluation of eluents for the recovery of an enveloped virus from hands by whole-hand sampling CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction An optimized method to detect influenza virus and human rhinovirus from exhaled breath and the airborne environment Evaluation of Filters for the Sampling and Quantification of RNA Phage Aerosols Southeastern Virginia using wastewater-based epidemiology Development of a novel method for simultaneous concentration of viruses and protozoa from a single water sample A review on recent progress in the detection methods and prevalence of human enteric viruses in water First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan Identification of SARS-CoV-2 in wastewater in Japan by multiple molecular assays-implication for wastewater-based epidemiology (WBE) Validation of Internal Controls for Extraction and Amplification of Nucleic Acids from Enteric Viruses in Water Samples Temporal dynamics of norovirus determined through monitoring of municipal wastewater by pyrosequencing and virological surveillance of gastroenteritis cases SARS-CoV-2 in wastewater: State of the knowledge and research needs Use of Murine Norovirus as a Novel Surrogate to Evaluate Resistance of Human Norovirus to Free Chlorine Disinfection in Drinking Water Supply System in India through detection of genetic material of SARS-CoV-2 First detection of SARS-CoV-2 in untreated wastewaters in Italy Minimized virus binding for tests of barrier materials Presence of SARS-Coronavirus-2 RNA in Sewage and Correlation with Reported COVID-19 Prevalence in the Early Stage of the Epidemic in The Netherlands Sensitive and specific quantitative detection of rotavirus A by one-step real-time reverse transcription-PCR assay without antecedent double-stranded-RNA denaturation Wastewater-Based Epidemiology Can Overcome Representativeness and Stigma Issues Related to COVID-19 R: A Language and Environment for Statistical Computing SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration Development of Genetic Diagnostic Methods for Novel Coronavirus 2019 (nCoV-2019) in Japan Cross-platform evaluation of commercial real-time reverse transcription PCR master mix kits using a quantitative 5′nuclease assay for Ebola virus Impact of repeated pressurization on virus removal by reverse osmosis membranes for household water treatment Viral multiplex quantitative PCR assays for tracking sources of fecal contamination Virological assessment of hospitalized patients with COVID-2019 SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases Survivability, Partitioning, and Recovery of Enveloped Viruses in Untreated Municipal Wastewater Shotaro Torii: Investigation, Resources, Funding Acquisition Writing -review & editing. The comparison of virus concentrations between concentrated samples and control allows for the evaluation of the whole process recovery ratio as described below. where C obs_WPC indicates the cDNA concentration of WPC in concentrated samples (copies/mL), C ini_WPC indicates the cDNA concentration of WPC in the control sample (copies/mL), x the concentration factor during the whole process (primary concentration, RNA extraction, and RT), C obs_MPC indicates the cDNA concentration of MPC in concentrated samples (copies/mL), C ini_MPC indicates the cDNA concentration of MPC in the control sample (copies/mL) and ALOD indicates the assay limit of detection defined as the minimum copy number with a 95% probability detection (copies/mL).	cache/cord-325479-2r4oomdp.txt	txt/cord-325479-2r4oomdp.txt
