This is an open access article under the terms of a license that permits non-commercial use, provided the original work is properly cited. © 2023 The Authors. Société Internationale d'Urologie Journal, published by the Société Internationale d'Urologie, Canada. SIUJ.ORG SIUJ • Volume 4, Number 4 • July 2023 Key Words Competing Interests Article Information Testicular cancer, germ cell tumors, cell-free DNA, circulating tumor DNA, liquid biopsy None declared. Received on December 7, 2022 Accepted on May 13, 2023 This article has been peer reviewed. Soc Int Urol J. 2023;4(4):287–292 DOI: 10.48083/IPCO3495 293 REVIEW — LIQUID BIOPSY The Role of Circulating Tumor DNA and Cell-Free DNA in the Management of Germ Cell Tumors: A Narrative Review Isabella Dolendo,1 Shanice Cox,2 Dhruv Puri,1 Aditya Bagrodia1,3 1Department of Urology, UC San Diego School of Medicine, La Jolla, United States 2 Department of Urology, Burnett School of Medicine at Texas Christian University, Fort Worth, United States 3 Department of Urology, University of Texas Southwestern Medical Center, Dallas, United States Abstract Liquid biopsy has demonstrated success as a diagnostic, prognostic, and therapy response monitoring tool in various cancers and could represent a rapid and minimally invasive alternative or complementary test for testicular germ cell tumors (GCTs). This article aims to review the current state of the research into circulating tumor DNA (ctDNA) and cell-free DNA (cfDNA) in testicular GCTs. Studies have confirmed the presence of ctDNA and cfDNA can be identified in peripheral blood samples of patients with testicular GCTs. Further research has attempted to optimize the methods for ctDNA detection in plasma to improve the sensitivity of these tests; however, a single method with high sensitivity and reliability has yet to be established. Previous studies have employes different methods for detecting cfDNA, including spectrophotometry, capillary electrophoresis, quantitative polymerase chain reaction (PCR), reverse transcription-polymerase chain reaction (RT-PCR), and whole genome sequencing. These studies have various elements of cfDNA examined such as total cfDNA quantity, methylation patterns, and specific mutations. Additional studies have investigated the efficacy of cfDNA detection in combination with other tests including miRNA analysis. The application of cfDNA as a biomarker has been rapidly expanding in several malignancies. However, there is a relative paucity of research on the clinical utility of cfDNA in testicular cancer, and many questions remain about the significance and feasibility of this biomarker in GCTs. Cell-free DNA shows promise as a biomarker to enhance detection and disease monitoring in testicular cancer, but robust studies are needed to develop an optimal and reproducible method for cfDNA detection in order to determine its clinical application in testicular cancer. Introduction The diagnostic evaluation of testicular cancer typically involves serum tumor markers including alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG), and lactate dehydrogenase (LDH)[1]. However, these markers are elevated in only about 40% of patients with testicular cancer[2]. Testicular biopsy is typically avoided because of the associated risk for tumor cell seedling and altered patterns of metastases[3]. To overcome these limitations, liquid biopsy has emerged as a recent development, providing a rapid, accurate, and noninvasive alternative to tissue biopsy and radical orchiectomy[4]. http://SIUJ.org mailto:bagrodia%40health.ucsd.edu?subject=SIUJ is considered to be tumor specific and uniquely identifiable, and its analysis can provide insights into disease burden and progression. Of the various cfDNA characteristics, cf DNA methylation and mutation analysis hold the most promise as cancer biomarkers[11]. Several studies have identified cf DNA in plasma samples of testicular cancer patients. Ellinger et al. collected serum samples from patients with semino- mas or nonseminomas and from healthy patients and isolated the cfDNA[1]. The investigators used RT-PCR to examine beta-actin DNA fragments (106 bp, 193 bp, and 384 bp) and found these fragments to be significantly increased in patients with testicular germ cell tumors (TGCTs) compared to disease-free controls. Boublikova et al. investigated total cf DNA using spectrophotome- try, capillary electrophoresis, and quantitative poly- merase chain reaction (qPCR) in the peripheral plasma of patients with TGCTs and controls[12]. They found that patients with TGCTs had significantly higher total cfDNA compared to controls. Studies have shown CTCs can also be detected in the peripheral samples of patients with TGCTs. Bokemeyer et al. detected CTCs in apheresis samples from 58% of patients with metastatic TGCTs undergoing high-dose chemotherapy and peripheral blood stem-cell (PBSC) transplantation[13]. Fan et al. detected beta-hCG mRNA in apheresis products using PCR[14], suggesting the presence of a significant number of CTCs or cell-free DNA rather than tumor cells. All patients with circulat- ing mRNA exhibited elevated serum beta- hCG levels, whereas only 46% of patients without circulating mRNA tested positive for beta- hCG. The findings also revealed a correlation between positive PCR results and higher serum beta- hCG levels at diagnosis[14]. Similarly, in a study by Hautkappe et al., all patients with circulating beta- hCG or AFP mRNA had elevated serum beta- hCG levels, while only 40% of patients with negative PCR results had positive serum beta- hCG[15]. A study conducted by Hildebrandt et al. demon- strated that reverse transcription-polymerase chain reaction (RT-PCR) targeting germ cell alkaline phospha- tase is highly sensitive in detecting residual GCT cells in peripheral blood. This method enabled the detection of one tumor cell in at least 106 mononuclear cells[16]. Yuasa et al. also used RT-PCR to detect malignant cells in the bloodstream by measuring the expression of AFP in the peripheral blood of patients with advanced stage testicular cancer. They found that this assay could detect a single cancer cell in 106 peripheral blood stem cells[17]. Further advancements in research have led to the development of techniques that enhance the detectabil- ity of ctDNA and CTCs in GCTs. Nastały et al. devel- oped a new assay using a label-free enrichment technique based on the physical properties of tumor cells. By using TABLE 1. List of testicular cancer MeSH entry terms and cell-free DNA–related search terms used to identify relevant literature in the Cochrane Review and PubMed databases Testicular cancer MeSH entry terms Cell-free DNA–related search terms Testicular Neoplasm Neoplasm, Testicular Testicular Tumors Neoplasms, Testis Neoplasm, Testis Testis Neoplasm Testis Neoplasms Testicular Tumor Tumor, Testicular Tumors, Testicular Neoplasms, Testicular Cancer of Testis Testis Cancer Cancer, Testis Cancers, Testis Testis Cancers Cancer of the Testes Cancer of the Testis Testicular Cancer Cancer, Testicular Cancers, Testicular Testicular Cancers Tumor of Rete Testis Rete Testis Tumor Rete Testis Tumors Testis Tumor, Rete Testis Tumors, Rete Biopsies, Liquid Biopsy, Liquid Liquid Biopsies Cell-Free Nucleic Acids Nucleic Acids, Cell-Free Circulating Cell-Free Nucleic Acid Circulating Cell-Free Nucleic Acid Circulating Nucleic Acids Acids, Circulating Nucleic Nucleic Acids, Circulating Cell-Free Nucleic Acid Cell-Free Nucleic Acid Nucleic Acid, Cell-Free Circulating Cell-Free Nucleic Acids Circulating Cell-Free Nucleic Acids Circulating Nucleic Acid Acid, Circulating Nucleic Nucleic Acid, Circulating Cell-Free DNA Cell-Free DNA DNA, Cell-Free cfDNA cirDNA Cell-Free Deoxyribonucleic Acid Acid, Cell-Free Deoxyribonucleic Cell-Free Deoxyribonucleic Acid Deoxyribonucleic Acid, Cell-Free Circulating DNA DNA, Circulating Cell-Free RNA Cell-Free RNA RNA, Cell-Free cfRNA cirRNA Cell-Free Ribonucleic Acid Acid, Cell-Free Ribonucleic Cell-Free Ribonucleic Acid Ribonucleic Acid, Cell-Free Circulating RNA RNA, Circulating DNA, Circulating Tumor Tumor DNA, Circulating Cell-Free Tumor DNA Cell-Free Tumor DNA DNA, Cell-Free Tumor Tumor DNA, Cell-Free cfDNA: cell-free DNA; cirRNA: circulating tumor DNA; MeSH: Medical Subject Heading. Liquid biopsy involves the extraction and analysis of biological samples, such as blood, urine, or saliva. Unlike tissue biopsy, which provides information confined to a specific region and fails to capture complex tumor heterogeneity, liquid biopsy isolates and analyzes tumor-derived or tumor-associated components that circulate in the bloodstream: circulating tumor cells (CTCs), circulating leukocytes, and tumor-derived circulating nucleic acids, such as cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), microRNA (miRNA), and noncoding RNA. This enables longitudinal moni- toring of cancer progression[4]. Cell-free DNA refers to fragmented DNA in the noncellular component of blood. Circulating tumor DNA consists of DNA fragments of 150 to 200 base pairs (bp) released into the peripheral blood from cancerous cells as a result of apoptosis, necrosis, or secretion[5]. The presence of tumor-specific genetic and epigenetic alter- ations in ctDNA makes ctDNA a promising biomarker. The concentration of ctDNA in blood plasma varies among cancer patients based on the type, location, and stage of the cancer and is typically low. The detection of somatic mutations, frequently single base-pair changes, copy number variations, or chromosomal rearrange- ments in ctDNA shows promise for early cancer diag- nosis, tumor dynamics assessment, minimal residual disease identification, and therapy monitoring. Cell-free DNA isolated from cancer patients can be used for various downstream applications, such as the investigation of mutations, copy number variations, gene fusions, and DNA methylation using various meth- ods including sequencing- or PCR-based approaches. ctDNA analysis can potentially overcome tumor hetero- geneity, which may not be captured by tissue sampling. Currently, there are 3 FDA-approved liquid biopsy tests: 2 cf DNA-based tests (Cobas EGFR Mutation Test v2 and Epi proColon) and one CTC-based test (CellSearch)[5]. Numerous ongoing clinical trials are employing cf DNA analysis in urologic malignan- cies. Mutations in fibroblast growth factor receptor-3 (FGFR3) in cf DNA were monitored in patients with advanced urothelial carcinoma undergoing treatment with an FGFR antagonist[6]. Patients with a decrease in mutations in cf DNA were found to correlate with a greater decrease in tumor size. Additionally, analysis of FGFR3 mutations in cf DNA were shown to predict disease progression ahead of imaging with computer- ized tomography (CT) scans. cfDNA has also been used in a study on a programmed death-ligand 1 (PD-L1) inhibitor in urothelial cancer by Powles et al.[7]. Those authors found a correlation between mutations found in ctDNA with mutations found in tumor DNA, suggesting a role for plasma-based biomarker screening in predict- ing immunotherapy response[7]. Cell-free and circulating tumor DNA are currently being used in clinical trials for urothelial carcinomas. For example, Huang et al. are recruiting for a study using ctDNA and urine tumor DNA as biomarkers for minimal residual disease and upper urinary tract urothelial carcinoma recurrence[8]. Van der Heijden et al. are monitoring ctDNA during follow-up of patients with urothelial carcinoma treated with ipilimumab and nivolumab[9]. Lolkema and van der Veldt are collecting ctDNA as part of their study on biomarkers in patients with advanced urothelial cancer treated with pembroli- zumab[10]. In this paper, we review the current state of research into liquid biopsies in GCTs. Methods A narrative review was conducted on September 24, 2022, by searching the PubMed and Cochrane Review databases without any limitation on the date range. Articles addressing cf DNA and ctDNA in testicular cancer were included in the review. The search terms used included all Medical Subject Heading (MeSH) entry terms for testicular cancer and cell-free DNA– related terms (Table 1). Relevant articles were also identified through the references of the included articles. All types of studies related to ctDNA and cf DNA in testicular cancer were included. Duplicate articles were excluded. Three reviewers (I.D., S.C., D.P.) independently screened the articles for inclusion. Any discrepancies were discussed to determine the final inclusion. Circulating Tumor DNA and Cell-Free DNA in Testicular Cancer Testicular cf DNA is thought to result from apoptosis during spermatogenesis. This can occur in both physiological and pathological conditions, including ma lignancy. In the case of ma lignancy, cf DNA Abbreviations AFP alpha-fetoprotein bp base pairs CTCs circulating tumor cells cfDNA cell-free DNA ctDNA circulating tumor DNA GCTs germ cell tumors hCG human chorionic gonadotropin LDH lactate dehydrogenase miRNA microRNA PCR polymerase chain reaction qPCR quantitative polymerase chain reaction RT-PCR reverse transcription-polymerase chain reaction TGCTs testicular germ cell tumors 295294 SIUJ.ORG SIUJ • Volume 4, Number 4 • July 2023SIUJ • Volume 4, Number 4 • July 2023 SIUJ.ORG REVIEW — LIQUID BIOPSY The Role of Circulating Tumor DNA and Cell-Free DNA in the Management of Germ Cell Tumors: A Narrative Review http://SIUJ.org http://SIUJ.org mutations. Tsui et al. identified cf DNA mutations in plasma samples and employed whole genome sequenc- ing to estimate the cfDNA tumor fraction[24]. By using cf-IMPACT, a tumor test capable of detecting genomic alterations in specific cancer-related genes, the research- ers found somatic mutations in 60% of samples from patients with TGCTs (n = 118). For the remaining samples without detected alterations and low tumor fraction, a more sensitive but less comprehensive test, MSK-ACCESS, was used for re-analysis. This latter test showed 14 additional samples with somatic muta- tions. Whole exome sequencing was then performed for samples without detected alterations but high tumor fraction, leading to the identification of 5 more samples with somatic mutations. In total, 90 of 118 samples (79%) exhibited somatic mutations across all 3 meth- ods. Overall, this study showed that cfDNA tumor frac- tion can guide further testing for somatic mutations in cfDNA to maximize mutation identification. Moreover, the identification of specific mutations has the potential to inform the selection of precision medicine treatments. The prognostic implications of the presence of cfDNA and ctDNA in GCTs remain unclear. Ellinger et al. found that levels of cell-free mtDNA did not correlate with disease severity, including tumor stage or lympho- vascular invasion, and Bokemeyer et al. showed that the presence of CTCs in transplanted PBSC apheresis products did not affect overall survival[13,23]. On the other hand, Hautkappe et al. demonstrated that 41.2% of patients with stage IIc/III GCTs had detectable mRNA of either AFP or beta- hCG compared to only 23.5% of patients with stage I disease[15]. In a clinical trial conducted by Reid et al., cell-free DNA is being investigated to determine whether cfDNA is detectable in the plasma of patients with platinum refractory/resistant GCTs. The researchers are further analyzing patients with detectable cf DNA for changes in cfDNA that are associated with clinical resistance to platinum (NCT0398087)[25]. The clinical management of TGCT has experienced limited progress in recent decades in integrating molec- ular diagnostic and prognostic tools into targeted thera- pies for affected patients[26]. Total cfDNA and changes in cfDNA levels have been shown to be associated with specific treatment responses, prognoses, and survival rates in various tumors including lung, pancreatic, colorectal, and other malignancies. While the utility of cfDNA in GCTs remains uncer- tain, circulating miR-371a-3p has recently captured attention as a robust biomarker with significant poten- tial to address clinical gaps in GCT management. In 2011, Murray and colleagues reported detectable serum miR-371a-3p in a 4-year-old boy with disseminated yolk sac tumor[27]. Subsequent to this, a large body of literature has consistently illustrated the utility of miR-371a-3p in differentiating GCTs from controls[28]. In the largest report to date, Dieckmann et al. analyzed a cohort of 616 GCT patients and found that miR-371a-3p exhibited a sensitivity of 90.1% and specificity of 94% for the diagnosis of GCT[29]. miR-371a-3p also demon- strated a sensitivity of 83% and specificity of 96% for identifying relapses, indicating its expanded clinical utility beyond diagnosis[29]. The predictive capability of miR-371a-3p for identifying relapses has been substan- tiated by Nappi et al. and Lafin et al.[30,31]. Leão et al. expanded on previous work by demonstrating that miR-371a-3p could also detect residual viable GCT after chemotherapy, with an area under the curve of 0.874, and with levels declining in response to treatment[32]. Apar t from identif y ing teratomas, miR-371a-3p shows potential as an important adjunct in the clini- cal management of GCTs. Currently, 2 clinical trials (NCT04914026, NCT04435756) are exploring the role of miR-371 in the management of GCTs[33,34]. Discussion The utility of circulating tumor DNA and cell-free DNA as diagnostic, prognostic, and therapeutic markers has been well-documented in various cancers. While the application of cf DNA as a biomarker has been rapidly expanding in several malignancies, relatively little research exists on the clinical utility of cfDNA in testicular cancer, leaving many questions unanswered about the significance and feasibility of this biomarker in TGCTs. Studies have demonstrated the presence of ctDNA and cfDNA in peripheral blood samples of patients with TGCTs. Efforts have been made to optimize the methods for ctDNA detection in plasma to enhance the sensitivity of these tests; however, a single method with high sensi- tivity and reliability has yet to be established. Previous studies have employed diverse methods for detecting cfDNA, including spectrophotometry, capillary electro- phoresis, qPCR, RT-PCR, and whole genome sequenc- ing. These studies have also examined various aspects of cf DNA, such as total cf DNA quantity, methylation patterns, and specific mutations (Table 2). Some stud- ies have evaluated the efficacy of cf DNA detection in combination with other tests including miRNA analysis. Many questions remain about the implementation of cfDNA and ctDNA in the clinical setting. To achieve the clinical validity and utility of liquid biopsy tests, it is necessary to assess the analytical validity of these tests, and then conduct prospective studies using the protocols that resulted in analytical validity. Analytical validity encompasses the accuracy, sensitivity, specific- ity, and robustness of the liquid biopsy test and relies on pre-analytical variables and protocols used for sample keratins 8, 18, and 19, and EpCAM as epithelial cell markers and SALL4 andOCT3/4 as germ cell markers, they achieved the detection of CTCs in 17.5% of periph- eral blood samples from testicular cancer patients, compared to only an 11.5% detection rate using the CellSearch assay[18]. Ruf et al. demonstrated the detec- tion of CTCs through alkaline phosphatase enzymatic activity and the use of anti-keratin, anti-EpCAM, and anti-SALL4 antibodies in immunocytochemistry[19]. Taken together, these studies highlight the wide vari- ety of tests developed to measure cf DNA and ctDNA in the peripheral samples of patients with TGCTs. However, the inability to designate a single test as the gold testing standard hinders comparison of cfDNA and ctDNA levels in TGCT patients across different stud- ies. The variation in testing methods poses a significant challenge in establishing clinical application of cfDNA and ctDNA in testicular cancer care. Clinical Utility of Circulating Tumor DNA and Cell-Free DNA in Testicular Cancer Circulating tumor and cell-free DNA offer multiple clinical applications in the context of testicular cancer, showing promise as diagnostic and prognostic tools. Various detection methods have been described in the literature. For example, Ellinger et al. used RT- PCR to isolate beta-actin DNA fragments in cf DNA, achieving a sensitivity of 84% and specificity of 97% in distinguishing patients with GCTs from healthy controls[1]. Even among patients with normal ranges of serum tumor markers, cfDNA exhibited a sensitivity of 84% and specificity of 97%, suggesting its potential utility as a biomarker for GCTs, particularly in those patients who do not have elevations in other serum tumor markers[1]. Investigations have also focused on total cfDNA and its 2 main fragments (360 bp and 180 bp) as targets for detection. Boublikova et al. used spectrophotometry, capillary electrophoresis, and qPCR to identify total cfDNA and found that the quantity of cfDNA does not offer a clear threshold to distinguish between TGCT patients and controls, thus limiting the sensitivity of this marker. However, they found that longer cf DNA fragments (360 bp) were present in 58% of patients with TGCTs but absent in controls[12], suggesting that target- ing longer cfDNA fragments instead of total cfDNA may be a viable approach. Further studies involving larger samples are necessary to establish an acceptable cutoff value. Methylation patterns in serum samples of patients with TGCTs have also shown promise as a biomarker. Lobo et al. evaluated hypermethylated RASSF1A in cfDNA as a diagnostic marker for testicular GCTs[20]. RASSF1A is a tumor suppressor gene that exhibits inactivation in many cancers. Promoter hypermethyl- ation of RASSF1A has been a useful marker in several solid malignancies and has also been detected in tissue samples of TGCTs[20]. To quantify hypermethylated RASSF1A, Lobo et al. used a novel droplet digital PCR method to analyze 102 serum samples from patients with GCTs and 29 samples from healthy young adult men[20]. They found that hypermethylated RASSF1A exhibited a sensitivity of 86.7%, surpassing the 65.5% sensitivity of AFP and hCG markers. They also found that combining hypermethylated RASSF1A with miR-371a-3p, a reliable microRNA biomarker for TGCTs, exhibited a sensitivity and specificity of 100%. This study identified a cfDNA target with high sensitivity and specificity, particularly in combination with a microRNA marker. Ellinger et al. also examined hypermethylation at RASSF1A, as well as methylation at APC, GSTP1, PTGS2, p14(ARF), and p16(INK)[21] using RT-PCR to evaluate samples from 73 patients with TGCTs and 35 individuals without disease. They found significantly higher rates of hypermethylation in the patients with testicular cancer across all examined gene sites. When combining multiple gene sites, the test achieved a sensi- tivity of 67% and specificity of 97%, surpassing the 58% sensitivity of conventional biomarkers (AFP, hCG, PLAP, LDH). In patients with normal laboratory results, the hypermethylation pattern test demonstrated a sensi- tivity of 97%, indicating its potential as a set of methyl- ation markers particularly useful for identifying disease in patients with normal laboratory markers. Kawakami et al. identified another potential cfDNA biomarker, the 5’ end of the XIST gene expressed in male germ cells[22]. This gene has been found to be hypomethylated in TGCTs. The investigators analyzed plasma samples from 24 patients with TGCTs and 24 controls and found 64% of patients with TGCTs had detectable unmethylated signals compared to none of the control samples. The presence of unmethylated signals was more common in patients with metastatic tumors (88%) compared to patients with nonmetastatic tumors (53%). Further research is needed to improve the sensitivity of tests for this marker and establish unmet- hylated XIST fragments as a diagnostic tool for TGCT. Ellinger et al. also investigated circulating cell-free mito- chondrial DNA in the serum of patients with seminoma and nonseminomas and found that cell-free mtDNA exhibited a sensitivity of 59.6% and 94.3%, respectively, in identifying patients with GCTs[23]. For patients without elevated serum tumor markers, cell-free mito- chondrial DNA demonstrated a sensitivity of 64.5% and specificity of 91.4%. Studies have investigated the use of the cell-free DNA tumor fraction and the identification of somatic 297296 SIUJ.ORG SIUJ • Volume 4, Number 4 • July 2023SIUJ • Volume 4, Number 4 • July 2023 SIUJ.ORG REVIEW — LIQUID BIOPSY The Role of Circulating Tumor DNA and Cell-Free DNA in the Management of Germ Cell Tumors: A Narrative Review http://SIUJ.org http://SIUJ.org References 1. Ellinger J, Wittkamp V, Albers P, Perabo FGE, Mueller SC, von Ruecker A, et al. Cell-free circulating DNA: diagnostic value in patients with testicular germ cell cancer. J Urol.2009;181(1):363–371. doi: 10.1016/j. juro.2008.08.118. PMID: 19010497. 2. Trigo JM, Tabernero JM, Paz-Ares L, García-Llano JL, Mora J, Lianes P, et al. Tumor markers at the time of recurrence in patients with germ cell tumors. Cancer.2000;88(1):162–168. doi: 10.1002/(sici)1097- 0142(20000101)88:1<162::aid-cncr22>3.0.co;2-v. PMID: 10618619. 3. Lobo J, Gillis AJM, Jerónimo C, Henrique R, Looijenga LHJ. 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Circulating tumor cells in patients with testicular germ cell tumors. Clin Cancer Res.2014;20(14):3830–3841. doi: 10.1158/1078-0432. CCR-13-2819. PMID: 24634372. 21. Ruf C, Nastał y P, Becker P, Isbarn H, Honecker F, Pantel K, et al. 703 Circulating tumor cells can be detected in patients with testicular germ cell tumors. J Urol.2013;189(4S):e289. doi: 10.1016/j.juro.2013.02.261. preparation and biomarker detection[35]. Pre-analytical variables are factors not associated with the disease that can impact the integrity of the bodily fluids or biomark- ers present therein and hence inf luence the analysis results. These variables can have a technical, biological, or environmental origin. The impact of pre-analytical variables is based on the biomarker and nature of the bodily fluid studied and should be thoroughly assessed for each newly developed liquid biopsy test[36,37]. Standard operating procedures for the complete work- flow of liquid biopsy tests are critical for advancing their clinical implementation[5]. Despite the success of cf DNA in multiple cancers, particularly lung and colon cancers, only 3 liquid biopsy tests have received FDA approval. The lack of TABLE 2. Summary of studies included in this narrative review highlighting the various detection methods and targets Study Detection method Target Bokemeyer et al.[13] RT-PCR beta- hCG, fibronectin (EDB variant), EGFR, CD44 (v8 to 10 variant), germ cell and placental alkaline phosphatase, human endogenous retrovirus type K (ENV and GAG), and XIST Boublikova et al.[12] Spectrophotometry, capillary electrophoresis, qPCR Total cfDNA and its 2 main fragments (360 bp and 180 bp) Ellinger et al.[23] RT-PCR cell-free mitochondrial DNA Ellinger et al.[1] RT-PCR 106 bp, 193 bp, and a 384 bp beta-actin DNA fragment Ellinger et al.[21] RT-PCR Hypermethylation at RASSF1A; methylation at APC, GSTP1, PTGS2, p14(ARF), and p16(INK) Fan et al.[14] PCR beta- hCG mRNA Hautkappe et al.[15] RT-PCR beta- hCG mRNA, AFP mRNA Hildebrandt et al.[16] RT-PCR Germ cell alkaline phosphatase Kawakami et al.[22] PCR Methylation of 5’ end of the XIST gene Lobo et al.[20] Droplet digital PCR Hypermethylated RASSF1A Nastał y et al.[18] Label-free enrichment technique, immunocytochemical staining Germ cell tumor (anti-SALL4, anti-OCT3/4) and epithelial cell–specific (anti- keratin, anti-EpCAM) antibodies Ruf et al.[19] Immunocytochemistry Anti-keratin, anti-EpCAM, and anti-SALL4 antibodies Tsui et al.[24] cf-IMPACT, MSK-ACCESS, whole genome sequencing cfDNA tumor fraction and somatic mutations Yuasa et al.[17] RT-PCR AFP cfDNA: cell-free DNA; mRNA: messenger RNA; PCR: polymerase chain reaction; RT-PCR: transcription-polymerase chain reaction. reproducibility in preclinical research may contribute to this poor clearance rate by the FDA. Future directions may focus on developing standardized methods for measuring cf DNA in plasma to ensure reliable results that provide clinical value. Standardization efforts may also help establish cutoff values for screening and prog- nostic purposes. Addressing the challenges of cf DNA detection, such as the low quantities of cfDNA in plasma and its rapid clearance of cfDNA to circulation, is crucial for the successful implementation of standardized meth- ods. Cell-free DNA holds promise as a biomarker to enhance detection and disease monitoring in testicu- lar cancer, but robust studies are needed to establish an optimal and reproducible method for cfDNA detection before determining its clinical application in the context of testicular cancer. 299298 SIUJ.ORG SIUJ • Volume 4, Number 4 • July 2023SIUJ • Volume 4, Number 4 • July 2023 SIUJ.ORG REVIEW — LIQUID BIOPSY The Role of Circulating Tumor DNA and Cell-Free DNA in the Management of Germ Cell Tumors: A Narrative Review http://SIUJ.org http://SIUJ.org 22. Lobo J, van Zogchel LMJ, Nuru MG, Gillis AJM, van der Schoot CE, Tytgat GAM, et al. Combining hypermethylated RASSF1A detection using ddPCR with miR-371a-3p testing: an improved panel of liquid biopsy biomarkers for testicular germ cell tumor patients. Cancers (Basel).2021;13(20):5228. doi: 10.3390/cancers13205228. PMID: 34680375; PMCID: PMC8534014. 23. Ellinger J, Albers P, Perabo FG, Müller SC, von Ruecker A, Bastian PJ. CpG island hypermethylation of cell-free circulating serum DNA in patients with testicular cancer. J Urol.2009;182(1):324–329. doi: 10.1016/j.juro.2009.02.106. PMID: 19447423. 24. Kawakami T, Okamoto K, Ogawa O, Okada Y. XIST unmethylated DNA fragments in male-derived plasma as a tumour marker for testicular cancer. Lancet.2004;363(9402):40–42. doi: 10.1016/S0140- 6736(03)15170-7. PMID: 14723995. 25. Ellinger J, Albers P, Müller SC, von Ruecker A, Bastian PJ. Circulating mitochondrial DNA in the serum of patients with testicular germ cell cancer as a novel noninvasive diagnostic biomarker. BJU Int.2009 Jul;104(1):48–52. doi: 10.1111/j.1464-410X.2008.08289.x. PMID: 19154496. 26. Tsui DWY, Cheng ML, Shady M, Yang JL, Stephens D, Won H, et al. Tumor fraction-guided cell-free DNA profiling in metastatic solid tumor patients. Genome Med.2021;13:96. doi: 10.1186/s13073-021-00898-8. PMID: 34059130; PMCID: PMC8165771. 27. Royal Marsden NHS Foundation Trust. Exploratory Study of Molecular Characterization in Patients With Metastatic Germ Cell Tumours Refractor y/Resistant to Platinum Treatment. CinicalTrials.gov identifier NCT03980587. Updated June 10, 2019. Accessed June 11, 2023. https://clinicaltrials.gov/ct2/show/NCT03980587 28. Lavoie JM, Kollmannsberger CK. Current management of disseminated germ cell tumors. Urol Clin North Am.2019;46(3):377–388. doi: 10.1016/j.ucl.2019.04.003. PMID: 31277732. 29. Murray MJ, Halsall DJ, Hook CE, Williams DM, Nicholson JC, Coleman N. Identification of microRNAs from the miR-371~373 and miR-302 clusters as potential serum biomarkers of malignant germ cell tumors. Am J Clin Pathol.2011;135(1):119–125. doi: 10.1309/ AJCPOE11KEYZCJHT. PMID: 21173133. 30. Gillis AJM, Rijlaarsdam MA, Eini R, Dorssers LCJ, Biermann K, Murray MJ, et al. Targeted serum miRNA (TSmiR) test for diagnosis and follow- up of (testicular) germ cell cancer patients: a proof of principle. Mol Oncol.2013;7(6):1083–1092. doi: 10.1016/j.molonc.2013.08.002. PMID: 24012110; PMCID: PMC5528443. 31. Dieckmann KP, Radtke A, Geczi L, Matthies C, Anheuser P, Eckardt U, et al. Serum levels of microRNA-371a-3p (M371 Test) as a new biomarker of testicular germ cell tumors: results of a prospective multicentric study. J Clin Oncol.2019;37(16):1412–1423. doi: 10.1200/JCO.18.01480. PMID: 30875280; PMCID: PMC6544462. 32. Nappi L, Thi M, Lum A, Huntsman D, Eigl BJ, Martin C, et al. Developing a highly specific biomarker for germ cell malignancies: plasma miR371 expression across the germ cell malignancy spectrum. J Clin Oncol.2019;37(33):3090–3098. doi: 10.1200/JCO.18.02057. PMID: 31553692; PMCID: PMC7351323. 33. Lafin JT, Singla N, Woldu SL, Lotan Y, Lewis CM, Majmudar K, et al. Serum microRNA-371a-3p levels predict viable germ cell tumor in chemotherapy-naïve patients undergoing retroperitoneal lymph node dissection. Eur Urol.2020;77(2):290 –292. doi: 10.1016/j. eururo.2019.10.005. PMID: 31699528; PMCID: PMC7756387. 34. Leão R, van Agthoven T, Figueiredo A, Jewett MAS, Fadaak K, Sweet J, et al. Serum miRNA predicts viable disease after chemotherapy in patients with testicular nonseminoma germ cell tumor. J Urol.2018;200(1):126–135. doi: 10.1016/j.juro.2018.02.068. PMID: 29474847. 35. Haukeland University Hospital. MicroRNA as Markers in Testicular Cancer. ClinicalTrials.gov identifier NCT04914026. Updated August 8, 2022. Accessed June 11, 2023. https://clinicaltrials.gov/ct2/show/ NCT04914026?cond=NCT04914026 36. SWOG Cancer Research Network. A Study of miRNA 371 in Patients With Germ Cell Tumors. ClinicalTrials.gov identifier NCT04435756. Updated June 9, 2023. Accessed June 11, 2023. https://clinicaltrials. gov/ct2/show/NCT04435756 37. Merker JD, Oxnard GR, Compton C, Diehn M, Hurley P, Lazar AJ, et al. Circulating tumor DNA analysis in patients with cancer: American Society of Clinical Oncology and College of American Pathologists Joint Review. J Clin Oncol.2018;36(16):1631–1641. doi: 10.1200/ JCO.2017.76.8671. PMID: 29504847. 38. Fleischhacker M, Schmidt B. Pre-analytical issues in liquid biopsy – where do we stand? J Lab Med.2020;44(3):117–142. doi: 10.1515/ labmed-2019-0167. 39. Ellervik C, Vaught J. Preanalytical variables affecting the integrity of human biospecimens in biobanking. Clin Chem.2015;61(7):914–934. doi: 10.1373/clinchem.2014.228783. PMID: 25979952. 300 SIUJ • Volume 4, Number 4 • July 2023 SIUJ.ORG REVIEW — LIQUID BIOPSY http://SIUJ.org