667 Aggregation and Feeding Behavior of the Formosan Subterranean Termite (Isoptera: Rhinotermitidae) on Wood Decayed by Three Species of Wood Rot Fungi by Mary L Cornelius*, Kelley S. Williams, Mary P. Lovisa & Anthony J. De Lucca II ABSTRACT Aggregation and feeding behavior of the Formosan subterranean termite, Coptotermes formosanus Shiraki, was evaluated on wood decayed by three species of fungus, the brown rot fungus, Gloeophyllum trabeum and two white rot fungi, Phanerochaete chrysosporium and Pycnoporus cinnibarinus. Although termites aggregated on decayed sawdust from all three species in at least some of the tests, sawdust decayed by P. chrysosporium elicited aggrega- tion behavior by termites over the greatest range of incubation periods. In some tests, termites avoided sawdust decayed by G. trabeum. Termite feeding on blocks decayed for 90 d was significantly greater than on control blocks for all three species of fungi, despite the significantly lower decay rate of P. cinnibarinus. Increasing our understanding of the interaction of termites with wood rot fungi could lead to the identification of chemicals that attract termites to bait stations. Key Words: Coptotermes formosanus, fungus, aggregation, consumption, decay INTRODUCTION Lignocellulose is the major component of plant cell walls. The Formosan subterranean termite, Coptotermes formosanus Shiraki, uses cellulases secreted from salivary glands and symbiotic flagellates to digest cellulose (Nakashima et al. 2002, Zhang et al. 2011). Although there is evidence that termites produce cellulases, hemicellulases, and lignases, lignin is not metabolized by termites and acts as a physical barrier to cellulases (Breznak & Brune 1994, Brune United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124 *Corresponding author: Mary.Cornelius@ars.usda.gov; This article presents the results of research only. Mention of a commercial or proprietary product does not constitute endorsement or recommendation by the USDA. 668 Sociobiolog y Vol. 59, No. 3, 2012 2006, Geib et al. 2008, Coy et al. 2010, Ke et al. 2011, Scharf et al. 2011). Wood rot fungi may facilitate termite digestion of cellulose by modifying or metabolizing lignin. Brown rot fungi circumvent the lignin barrier and metabolize cellulose and hemicellulose without removing the lignin. Therefore, lignin remains the major component of plant cell walls degraded by brown rot fungi. However, brown rot fungi modify the lignin by demethylation and oxidation (Green III & Highley 1997). In contrast, white rot fungi simultaneously degrade the three major components of the plant cell wall: lignin, cellulose, and hemi- cellulose (Geib et al. 2008). Two species of white rot fungi have different enzyme systems for lignin degradation. Ligninolytic activity by, Phanerochaete chrysosporium Burdsall, is closely correlated with secretion of two specific per- oxidases, lignin peroxidase (LiP) and manganese peroxidase (MnP), whereas neither LiP nor MnP was detected in the ligninolytic activity of the white rot fungus, Pycnoporus cinnabarinus ( Jacq.:Fr.) Karst. Ligninolytic activity by P. cinnabarinus was characterized by a single dominant laccase (Eggert et al. 1996, Alves et al. 2004). Many studies have documented the preference of subterranean termites for wood decayed by the brown rot fungus, Gloeophyllum trabeum (Persoon: Fries) Murrill over sound wood (Amburgey 1979, Lenz et al. 1991). Studies have also demonstrated that chemicals in G. trabeum elicit trail following and directional tunneling behavior (Esenther et al. 1961, Matsumura et al. 1968, Rust et al. 1996, Su 2005). Other researchers have found that the interaction between subterranean termites and G. trabeum is antagonistic because of the competition for cellulose ( Jayasimha & Henderson 2007a, 2007b). In previous research, the Formosan subterranean termite has shown a significant preference for spruce, Picea sp., sawdust inoculated with both the brown rot fungus, G. trabeum and the white rot fungus, P. chrysosporium, over control sawdust. However, termites strongly preferred sawdust inocu- lated with P. chrysosporium over sawdust inoculated with G. trabeum. Also, there was significantly greater aggregation of termites on spruce sawdust that had only been decayed for three weeks by G. trabeum compared to sawdust decayed for 12 week (Cornelius et al. 2002). The objective of this study was to examine the interaction of Formosan subterranean termites with three species of wood rot fungi, the brown rot 669 Cornelius, M.L. et al. — Feeding Behavior of Termites on Decayed Wood fungus, G. trabeum and two species of white rot fungi, P. chyrsosporium and P. cinnabarinus at different stages of decay. Because the two species of white rot fungi use different enzyme systems for lignin degradation, their effect on termite behavior may be different. This study examines the aggregation and feeding behavior of Formosan subterranean termites on wood decayed by these three species of wood rot fungi. MATERIALS AND METHODS Termite collection and fungus cultures Termites were collected from field colonies in City Park, New Orleans, LA, using cylindrical irrigation valve boxes (22.5 by 14.8 cm; NDS, Lindsay, CA) buried in the ground so that the lids are level with the surface of the soil and filled with blocks of wood (spruce [Picea sp.]). Collections from differ- ent stations in the same section of City Park were considered to be separate colonies based on a mark-release-recapture technique using the dye markers Nile Blue A and neutral red (Sigma- Aldrich, Milwaukee, WI) to determine which stations were part of a single, interconnected tunneling system. Any termites from stations containing dyed termites were considered to be part of the same colony as the termites in the station from which the dyed termites were released. Termites were kept in the lab in 5.6-L covered plastic boxes con- taining moist sand and blocks of spruce until they were used in experiments. Termites were used for experiments within two months of collection. The brown rot fungus, G. trabeum (Madison 617), the white rot fungus, P. chrysosporium (46235) and the white rot fungus, P. cinnabarinus (10242) were obtained from the American Type Culture Collection (ATCC, Manas- sas, VA). Fungal inoculation of sawdust Northern red oak, Quercus rubra L., sawdust was inoculated with the mycelium from one of the three species of fungus and decayed for different incubation periods (7, 14, 30, 60, 90, 120, or 150 d). There was no sporula- tion by any of the fungi in any of the tests. Termites were only exposed to mycelium. Nutrient plates were inoculated with one of the fungus species and placed in an incubator set at a temperature of 25° C with a photoperiod of 12 :12 (L:D) h for 3 d. For P. chrysosporium, potato dextrose agar (PDA) 670 Sociobiolog y Vol. 59, No. 3, 2012 plates were used and for G. trabeum and P. cinnabarinus, potato dextrose yeast (PDY) plates were used. Five liters of nutrient broth were inoculated with (1-cm square) plugs from the plates at a ratio of four plugs per liter of broth. The flasks were incubated at 25º C in an orbital shaker at 120 rpm. Phanerochaete chrysosporium was cultured in Sabouraud dextrose broth for 7 d, whereas G. trabeum and P. cinnabarinus were cultured in PDY broth for 14 d. Afterwards, the fungal biomass was strained through a wire mesh strainer, and finally vacuum filtered through a Buchner funnel using a sterile Whatman #4 filter paper. Sawdust was placed in an autoclavable polypropylene vent bags (32 by 40 cm) (Unicorn Imp. and Mfg. Corp., Commerce, TX) with a single 0.2 micron filter (7 by 25 cm), 100 grams per bag. The bags were heat- sealed and autoclaved vent side up, for 1 h on each of two consecutive days. An amount of mycelium equal to 43.4 g of mycelia per 100 g of sawdust was weighed and suspended in 18 ml of sterile water and blended in a Stomacher 400 Mark II Lab Blender (Spiral BioTech, Bethesda, MD) on high for 60 s. After the autoclaved bags were cooled to room temperature, one corner of the bag was cut open, and the filtered mycelium was added to the bag using a pipette. After the sawdust was inoculated with the filtered mycelium, the opening in the vent bag was heat-sealed. The bags were kept in an incubator set at 25º C with 12 h photoperiod for different lengths of time (7, 14, 30, 60, 90, 120, or 150 d). Aggregation behavior on decayed sawdust Tests were conducted to determine if termites aggregated on sawdust inoculated with living fungal mycelium after different incubation periods. Different batches of sawdust were inoculated at each incubation period to evaluate variability between different batches decayed for the same incuba- tion period. Three plastic screwtop containers (5cm diam and 4.7 cm height) were connected with 5 cm length pieces of PVC tubing. Each distal container was filled to a height of 1 cm with either treated or control sawdust or with sawdust decayed by two different fungus species. A soil moisture meter (Spectrum Technologies, Plainfield, IL) was used to make sure that moisture levels were equivalent in treated and control sawdust. The center container was filled with 10 g of sand, moistened with 2 ml of distilled water. For each replicate, termites (190 workers and 10 soldiers) were released into the center 671 Cornelius, M.L. et al. — Feeding Behavior of Termites on Decayed Wood container. For each test, termites were collected from four different colonies, with three replicates of each colony. For 7 d, containers were kept in an unlit environmental chamber set at 28°C, 97% RH. At the end of the test, the number of termites in the tubing and in each container was counted. Two tests were conducted with the first batch of sawdust decayed by G. trabeum for 30 d and 60 d because the behavior of termites toward the sawdust decayed for 30 d was different than toward the sawdust decayed for 60 d. Tests were repeated with this batch to evaluate this change in termite behavior towards the decayed sawdust. Fungus inoculation of wood blocks Blocks of red oak (4 cm by 3.5 cm by 1 cm) were oven-dried at 90° C for 24 h, weighed, and numbered. Blocks were then soaked in water for 3 d, wrapped in moist paper towels and two layers of aluminum foil, and then autoclaved for 60 minutes on two consecutive days. After cooling, each individual block was placed in a Petri dish (100 mm by 20 mm) containing PDA inoculated with one of the three fungus species. Block numbers and fungus species were written on the lid of each Petri dish. Fungus-inoculated blocks were kept in an environmental chamber set at a temperature of 25° C with 12 h photoperiod for 30, 60, or 90 d. The fungal mycelium of each species gradually covered the block during the incubation period. At the end of each time period, blocks were cleaned, oven-dried at 90° C for 24 h, and re-weighed. Decay rates were determined by calculating the weight loss of each block during the incubation period. Feeding behavior on decayed blocks A no-choice test was conducted using blocks decayed by one of the three fungus species for 30 d, 60 d, or 90 d and undecayed control blocks. The as- say was conducted using polystyrene, cylindrical screwtop containers (9 cm high by 7cm diam.) (Consolidated Plastics, Twinsburg, Ohio) filled with 50 g of sand (Play Sand, Quikrete, Atlanta, GA) and moistened with 10 ml of distilled water. After weight loss due to decay was determined, a block was placed on top of the sand in each container. For each replicate, termites (190 workers and 10 soldiers) were released into the container. For six weeks, containers were kept in an unlit environmental chamber set at 28°C, 97% RH. After six weeks, blocks were cleaned, oven-dried at 90° C for 24 h, and 672 Sociobiolog y Vol. 59, No. 3, 2012 re-weighed. Wood consumption was determined by calculating the weight loss of each block. Termites were collected from three different colonies, four replicates of each colony for each fungus species at each decay period, except that there were only two replicates of each colony for control blocks and for P. chrysosporium and G. trabeum at 30 and 60 d. Statistical analysis In aggregation assays, the number of termites in treated and control con- tainers was compared using a paired choice t-test. Termites, located in the tubing and the center container, were not included in the analysis. In the no- choice feeding test, proportional weight loss due to decay and feeding, and proportional survival were compared for each treatment (fungus species + decay period) and for each fungus species at each decay period (30, 60, or 90 d) separately using a one-way Kruskal-Wallis ANOVA. Means were separated by Dunn’s test with ranked sums due to unequal sample sizes. If the tests for both normality and equal variances passed, a one-way ANOVA was used and means were separated with Dunn’s test (Systat Software 2008). RESULTS AND DISCUSSION Aggregation behavior on decayed sawdust Average survival of termites in the aggregation tests was 92.4 ± 2.3. Sawdust decayed by P. chrysosporium had a highly significant effect on aggregation be- havior for all batches and incubation periods from 7 d -120 d. When sawdust was decayed for 150 d, there was no significant difference in the number of termites on decayed and control sawdust (Table 1). Termite responses to sawdust decayed by G. trabeum were highly variable. In the first batch, termites aggregated on the control sawdust when sawdust was decayed for 30 d (P = 0.05). In contrast, termites aggregated on sawdust decayed for 60 d. When these tests were repeated with sawdust from the same bags, termites aggregated on control sawdust after an incubation period of 30 d (P =0.003) and aggregated on the decayed sawdust after an incubation period of 60 d (P = 0.001). In the second batch, termites aggregated on saw- dust decayed for 30 d, and showed no response to sawdust decayed for 40, 50, or 60 d. In the third batch, they aggregated on control sawdust compared to sawdust decayed for 14 d. Termites also appeared to aggregate on control sawdust after 40 d (P =0.05) (Table 2). Aggregation behavior on sawdust 673 Cornelius, M.L. et al. — Feeding Behavior of Termites on Decayed Wood Table 1. Mean (±SE) number of termites in containers filled with red oak sawdust inoculated with Phanerochaete chrysosporium and decayed for different lengths of time compared with containers filled with control sawdust after 7 d. Mean (±SE) Number of Termites in Containers Incubation Time (days) Fungus Control P Value1 Batch 1 7 139.9 ± 11.6 4.7 ± 1.1 <0.0001 14 110.8 ± 6.2 30.6 ± 5.7 <0.0001 Batch 2 14 116.7 ± 7.6 41.0 ± 8.4 <0.0001 30 135.8 ± 11.9 13.7 ± 3.9 <0.0001 40 110.9 ± 9.5 30.6 ± 7.9 <0.0001 50 99.5 ± 9.4 32.8 ± 5.2 <0.0001 60 125.6 ± 7.6 19.6 ± 4.6 <0.0001 Batch 3 14 127.5 ± 8.7 12.2 ± 3.2 <0.0001 30 112.2 ± 12.1 19.6 ± 4.5 <0.0001 60 131.4 ± 9.4 14.2 ± 4.3 <0.0001 90 98.3 ± 9.9 28.7 ± 5.6 <0.001 120 123.2 ± 4.2 19.7 ± 3.0 < 0.001 150 87.4 ± 10.5 55.6 ± 11.4 0.12 1The number of termites in treated and control containers were compared using a paired choice t-test. Table 2. Mean (±SE) number of termites in containers filled with red oak sawdust inoculated with Gloeophylum trabeum and decayed for different lengths of time compared with containers filled with control sawdust after 7 d. Mean (±SE) Number of Termites in Containers Incubation Time (days) Fungus Control P Value1 Batch 1 14 26.7 ± 12.3 67.4 ± 16.9 0.14 30 (test 1) 21.5 ± 6.7 63.3 ± 18.3 0.05 30 (test 2) 25.6 ± 7.5 81.3 ± 10.4 0.003 60 (test 1) 67.8 ± 14.2 23.8 ± 10.8 0.04 60 (test 2) 99.3 ± 13.6 31.8 ± 6.3 0.001 Batch 2 30 68.0 ± 10.7 25.9 ± 5.1 0.01 40 58.2 ± 11.3 47.5 ± 10.8 0.63 50 46.9 ± 9.8 50.2 ± 9.9 0.84 60 37.1 ± 6.6 26.8 ± 6.8 0.30 Batch 3 14 46.3 ± 9.7 79.8 ± 8.5 0.08 30 38.2 ± 5.0 53.4 ± 8.6 0.17 40 46.1 ± 8.2 82.2 ± 11.5 0.05 50 58.4 ± 7.2 58.1 ± 7.0 0.97 60 42.4 ± 8.3 61.2 ± 5.2 0.09 1The number of termites in treated and control containers were compared using a paired choice t-test. 674 Sociobiolog y Vol. 59, No. 3, 2012 decayed by G. trabeum was not consistent between batches and did not cor- respond with incubation period. In some cases, termites aggregated on control sawdust. Because termites were exposed to living fungal mycelium in these tests, fungal growth rates were not measured. However, there may have been substantial differences in growth rates of G. trabeum between batches. Because G. trabeum does not metabolize lignin, it can have a negative effect on termites by depleting the cellulose without removing the lignin. Therefore, the range of conditions under which termites would aggregate on sawdust decayed by G. trabeum appears to be much narrower than for P. chrysosporium. Termites aggregated on sawdust decayed by P. cinnabarinus for 14 d in both batches, but did not show a significant response to sawdust decayed for longer incubation periods in either batch (Table 3). In choice tests of sawdust decayed by either P. chrysosporium or P. cinnabarinus for the same length of time, termites only displayed significant aggregation behavior on P. chrysosporium when sawdust was decayed for 120 d. Termites were dis- tributed equally between the two containers of sawdust decayed by the two fungi when sawdust was decayed for 14 or 60 d (Table 4). These were the first tests conducted to evaluate the response of termites to P. cinnabarinus. Although many factors, such as fungal growth rates and wood species, affect the interaction of subterranean termites with wood rot fungi, P. cinnabarinus did elicit aggregation behavior in termites when sawdust was only decayed for 14 d, but not when sawdust was decayed for a longer period. Sawdust decayed by P. chrysosporium elicited aggregation behavior by Formosan subterranean termites the most consistently and over the greatest range of incubation periods of the three fungus species tested. Aggregation behavior of termites on a food source could increase the efficacy of baiting programs for termite control by increasing consumption of toxic baits. Feeding behavior on decayed blocks In comparisons of rates of decay and feeding for the three fungi at each decay period, percent weight loss due to decay was significantly different at 30 d (H = 12.0, df = 2, P = 0.002), 60 d (H = 12.4, df = 2, P = 0.002), and 90 d (H = 19.7, df = 2, P < 0.001), but percent weight loss due to termite feeding was not significantly different at 30 d (F 2, 23 = 1.5, P = 0.24), 60 d (F 2, 23 = 1.4, P = 0.27), or 90 d (F 2, 35 = 3.1, P = 0.06). Percent weight loss due to 675 Cornelius, M.L. et al. — Feeding Behavior of Termites on Decayed Wood decay by P. cinnabarinus was significantly less than percent weight loss due to decay by the other two species at all three decay periods (Table 5). In comparisons of rates of survival, decay and feeding over all treatments, there were no significant differences in the survival of termites in containers with different treatments (H = 15.6, df = 9, P = 0.08). There were significant differences in the percent weight loss of blocks due to fungal decay (H = 56.5, df = 8, P < 0.001) and due to feeding damage (H = 51.2, df = 9, P < 0.001) for the different treatments. Percent weight loss due to decay was significantly greater for P. chrysosporium and G. trabeum after 90 d decay compared to percent weight loss for P. cinnabarinus at 90 d. In addition, percent weight loss due to decay by P. chrysosporium was also significantly greater after 60 Table 3. Mean (±SE) number of termites in containers filled with red oak sawdust inoculated with Pycnoporus cinnabarinus and decayed for different lengths of time compared with containers filled with control sawdust after 7 d. Mean (±SE) Number of Termites in Containers Incubation Time (days) Fungus Control P Value1 Batch 1 14 125.5 ± 11.4 62.3 ± 9.8 0.01 60 95.3 ± 16.4 48.2 ± 10.8 0.07 120 93.5 ± 15.3 80.0 ± 13.1 0.64 Batch 2 14 131.3 ± 12.1 30.0 ± 7.1 0.0001 30 82.0 ± 12.5 73.1 ± 9.4 0.65 120 68.2 ± 9.9 55.8 ± 8.1 0.32 1The number of termites in treated and control containers were compared using a paired choice t-test. Table 4. Mean (±SE) number of termites in containers filled with red oak sawdust inoculated with Pycnoporus cinnabarinus compared with containers filled with Phanerochaete chrysosporium when inoculated sawdust was decayed for different lengths of time after 7 d. Mean (±SE) Number of Termites in Containers Incubation Time (days) Pycnoporus Phanerochaete P Value1 14 68.3 ± 12.2 76.3 ± 18.1 0.79 60 80.5 ± 6.5 95.8 ± 5.6 0.22 120 31.0 ± 8.7 145.8 ± 8.5 <0.001 1The number of termites in treated and control containers were compared using a paired choice t-test. 676 Sociobiolog y Vol. 59, No. 3, 2012 d than percent weight loss for P. cinnabarinus at 60 d. Termite feeding on decayed blocks was significantly greater than on control blocks for all three fungi after 90 d of decay (Table 5). The effect of decay on feeding rates by termites is complex and affected by multiple factors. Although termites avoided sawdust decayed by G. trabeum in some aggregation tests, termite consumption of blocks decayed by G. trabeum was significantly greater than consumption of control blocks after 90 d of decay. Even though G. trabeum does not metabolize lignin, it seems to cause chemical changes to blocks that increase feeding rates by Formosan subterranean termites. The chemical modification of lignin by G. trabeum appears to facilitate termite feeding under at least some conditions. By re- moving cellulose while leaving the lignin behind, G. trabeum gradually makes the wood less palatable to termites as the rate of decay increases (Lenz et al. 2007, Cornelius et al. 2002). The weight loss of blocks due to decay by P .cinnabarinus was much lower than the weight loss due to decay by the other two fungus species. Even though weight loss of red oak blocks due to P. cinnabarinus was < 3% for all three incubation periods, the fungus may have been causing chemical changes to the blocks that facilitated termite feeding. After 90 d of incubation, wood consumption on decayed blocks was 12.6% compared to 4.8% on control blocks. Other studies have also demonstrated that termite feeding rates on decayed blocks do not always correlate directly with amount of decay or length of incubation periods (Amburgey & Smythe 1977, Lenz et al. 1980). Although there are many factors that could influence the differences in termite behavior towards the two white rot fungi, P. chrysosporium and P. cinnabarinus, the difference in the enzymatic pathways utilized for lignin degradation may be important. Further research is needed to elucidate the factors affecting termite aggregation and feeding behavior towards wood rot fungi. The interactions of termites with wood rot fungi range from obligate mutualism (Darlington 1994, Aanen et al. 2002) to antagonism (Becker 1976, Amburgey & Beal 1977, Jayasimha & Henderson 2007a, 2007b). By breaking down or chemically modifying lignin, wood rot fungi can facilitate the ability of termites to consume lignocellulose. Because of their symbiotic association with lignin-degrading fungi, fungus-growing termites are able to utilize virtually all of the lignocellulose they consume and they expel only a 677 Cornelius, M.L. et al. — Feeding Behavior of Termites on Decayed Wood small volume of feces (Darlington 1994). In an analysis of feces of Formosan subterranean termites fed southern pine, Pinus australis F. Michx, it was de- termined that lignin was modified, but not degraded, and that it retained its aromatic properties (Ke et al. 2011). Scharf et al. (2011) provide evidence that termites could gain a fitness advantage by utilizing gut symbionts to overcome end-product inhibition caused by the degradation of lignin. Formosan subter- ranean termites may be able to utilize a greater percentage of lignocellulose from decayed wood due to the biochemical changes in the wood. Therefore, feeding on decayed wood may provide a fitness advantage by allowing the wood rot fungi to partially degrade lignin prior to consumption. Many researchers have investigated the potential of using attractants from wood rot fungi in termite baits (Esenther & Beal 1974, Amburgey et al. 1981, Rust et al. 1996, Getty & Haverty 1998, Cornelius et al. 2009). However, optimal conditions for eliciting aggregation and feeding behavior by termites depends on many factors including, decay rate, wood species, and differences in fungal strains and species. Elucidation of the biochemical changes in wood Table 5. Percent (±SE) weight loss of red oak wood blocks decayed by three species of fungi for 30, 60, or 90 d. Percent (±SE) weight loss of blocks due to termite feeding and percent (±SE) termite survival in a no-choice feeding test after six weeks. Percent (±SE) Weight Percent (±SE) Weight Loss Loss of Blocks Due to Percent (±SE) Fungus Species of Blocks Due to Decay Termite Feeding Termite Survival Undecayed control blocks ------------- 4.8 ± 0.3A 78.5 ± 1.5 30 d decay Phanerochaete chrysosporium 5.4 ± 0.4aABC 10.9 ± 0.6A 88.7 ± 1.5 Gloeophylum trabeum 5.3 ± 0.3aABC 9.8 ± 0.2A 86.0 ± 1.8 Pycnoporus cinnabarinus 2.4 ± 0.5bA 10.7 ± 0.4A 86.9 ± 1.7 60 d decay Phanerochaete chrysosporium 8.1 ± 0.8aC 9.5 ± 1.7A 85.3 ± 4.6 Gloeophylum trabeum 7.9 ± 0.5aBC 9.5 ± 1.1A 83.6 ± 7.7 Pycnoporus cinnabarinus 2.9 ± 0.7bAB 11.3 ± 0.4A 87.2 ± 1.3 90 d decay Phanerochaete chrysosporium 11.11 ± 3.1aC 15.4 ± 1.1B 85.4 ± 1.7 Gloeophylum trabeum 10.3± 0.4aC 14.5 ± 0.7B 87.8 ± 1.0 Pycnoporus cinnabarinus 2.6 ± 0.1bAB 12.6 ± 0.4B 88.0 ± 0.9 Means followed by the same lowercase letter within an incubation period within a column were not significantly different (Dunn’s test: P ≥ 0.05). 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