703 Diversity of Epigeal Ants (Hymenoptera: Formicidae) in Urban Areas of Alto Tietê by D. R. de Souza1; S. G. dos Santos1; C. de B. Munhae2 & M. S. de C. Morini1 ABSTRACT The objective of this study was to conduct an inventory of the ant fauna, evaluating indicators of diversity, litter community composition and similar- ity among seven areas located around the city of Mogi das Cruzes (SP). In each area 20 pitfalls were distributed, which remained in the field for seven days. Four samplings were performed, two in the rainy season and two during dry season. In total there were 92 recorded species, 36 genera, 19 tribes and seven subfamilies. The most frequent species belong to the genera Pheidole and Camponotus, both common in the Neotropics. Differences were ob- served in species composition with the formation of two groups, one under the influence of the Atlantic Forest and other in the urban region. Only one exotic species, Paratrechina longicornis, was recorded. The results indicate the importance of the forest surrounding the city to maintain the biological diversity of ant communities. Keywords: anthropization, communities, Atlantic Forest, exotic ants, Serra do Itapeti INTRODuCTION urban ecosystems are characterized as spatially heterogeneous and tempo- rally dynamic sites, being usually recognized as areas under deep and constant human activities (McIntyre et al. 2001). These sites are effectively synonymous of disorder and reduction of biological diversity (Murphy 1988; McIntyre et al. 2001; Yamaguchi 2004; 2005). They are very similar all over the world in relation to the structure and function and differ only on the geographic location and size (Savard et al. 2000). 1universidade de Mogi das Cruzes, Núcleo de Ciências Ambientais, Laboratório de Mirmecologia. Av. Dr. Cândido Xavier de Almeida Souza n. 200. 08780-911. Mogi das Cruzes, SP, Brasil. 2universidade Estadual Paulista, Instituto de Biologia, Centro de Estudos de Insetos Sociais. Avenida 24 n. 1515. 13506-725. Rio Claro, SP, Brasil. Corresponding author. E-mail: morini@umc.br 704 Sociobiolog y Vol. 59, No. 3, 2012 Some cities still have areas of native vegetation that represent important refuges for plants and animals (Rodrigues et al. 1993). This is the case with the city of Mogi das Cruzes, located in the Alto Tietê River Basin and Pro- tected by the Watershed Law (Law N0 9.866). The region is composed of the main Atlantic Forest remnants in the southeastern Brazil and at the same time it has an expressive population and industrial development (Candido et al. 2010). Ants are among the most abundant insects in urban environments (McIntyre et al. 2001; Holway et al. 2002; López-Moreno et al. 2003). The high diversity, numerical dominance and biomass in almost all habitats, ease of sampling and species identification, the presence of stationary nests, which allows the re-sampling over time (Alonso & Agosti 2000) make ants good indicators of biological diversity in urban areas (Arcila et al. 2003). These insects also play critical functions for ecosystems, such as cycling of nutrients (Hölldobler & Wilson 1990; Folgarait 1998; Sanders & Van Veen 2011) and interaction with other organisms (Schultz & McGlynn 2000). Whereas the first step towards the conservation of certain areas or their use in a sustainable way are studies on biological diversity (Scott et al. 1987) and the importance of Formicidae ecosystems, this paper aims at presenting a list of species and descriptively assessing richness, diversity and similarity of communities around the city of Mogi das Cruzes. The urban area in this city grows around the Serra do Itapeti, which is a remnant of Atlantic forest with high biodiversity (Morini & Miranda 2011) and its conservation is of paramount importance. MATERIALS AND METHODS Samplings were made around the city of Mogi das Cruzes (S 23º52’ 22”; W 46°18’ 55” / 742 m), which is located in the eastern region of São Paulo city with 721 km2. Seven sampling areas were delimited along a transect, whose ends are located in an urban park (Parque Leon Feffer) and a Conservation unit (Parque Natural Municipal Francisco Affonso de Mello) (Table 1). In all areas, samplings were performed at sites under anthropogenic influence. Four samplings were carried out in each area, two in the rainy season and two in the dry season (Minuzzi et al. 2007). Ants were collected with pitfall 705 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê (N = 20), distributed every 20 m (Baccaro et al. 2011). Traps remained in field for seven days. The material was initially classified into subfamilies according to the proposal from Bolton (2003), identified at the level of genus and named according to Bolton (1994), Baroni-urbani & De Andrade (2007) and Lapolla et al. (2010) and subsequently named into morphospecies comparing specimens with those from the Formicidae collection of Alto Tietê. The taxa number- ing sequence is according to this collection. The species were identified by comparison with specimens deposited in the Zoolog y Museum, university of São Paulo (MZuSP). Vouchers were deposited at the university of Mogi das Cruzes (SP). Richness was defined as the number of species and the abundance as the number of individuals of each species collected. The relative frequency of occurrence was based on the presence and absence data. We calculated the estimated richness (Chao 2) and compared between different sampling areas using the Kruskal-Wallis test; the diversity indices of Shannon-Wiener (H ') and evenness (E). Patterns of species composition and community structure were compared between sampling sites through ordination analysis (non-metric multidimensional scaling - NMDS). A similarity dendrogram was constructed Table 1. Characterization of sampling sites in the city of Mogi das Cruzes (SP). Area Location Sampling Site characteristics PLF-Parque Leon Feffer S 23°31’37.56”; W 46°13’45.28” It is an urban park, whose vegetation is found in different degrees of regeneration CEC - Centro Esportivo Colégio Joana D’arc S 23°31’19.36”; W 46°13’14” Presence of grasses and a few tree species PNJ - Parque Nagib Najar S 23°31’17”; W 46°13’01” It is an urban park, whose vegetation is at various stages of regeneration AuI – urbanized area I S 23°30’49.24”; W 46’12’52.10” It is a country club, near a forest area; presence of grasses AuII – urbanized area II S 23°30’37.22”; W 46°12’32.22” Area near a highway; presence of grasses AP – Private Area S 23°30’10.97”; W 46°11’55.55” Little native vegetation, presence of grasses and exotic species PNMFAM - Parque Natural Municipal Francisco Affonso de Mello S 23°29’17”; W 46°11’43” It is a Conservation unit, consisting of secondary vegetation of Atlantic Forest at advanced stages of regeneration 706 Sociobiolog y Vol. 59, No. 3, 2012 using Bray-Curtis as dissimilarity measure for the analysis of clusters formed by the complete connection method. The sotfware R (Oksanen et al. 2009), EstimateS version 8.2 (Colwell 2009), Biostat (Ayres et al. 2007) and DivEs (Rodrigues 2005) were used for analyses. RESuLTS AND DISCuSSION The extensive inventory of epigaeic ant communities in areas surrounding the city of Mogi das Cruzes showed similar diversity in fauna and composed of 92 species, 36 genera, 19 tribes and seven subfamilies (Table 2). Myrmici- nae was the richest subfamily. The total richness recorded has been possibly influenced by the Atlantic Forest surrounding the city (Oliveira & Campos- Farinha 2005). The richest genera were Pheidole and Camponotus with total of 24 and 14 species, respectively. These genera are characterized by high species richness and abundance in different environments, being common in the neotropi- cal region (Ward 2000). Species such as Linepithema neotropicum Wild and Gnamptogenys striatula Mayr, common in the study sites, are found in the litter of the Atlantic Forest in region (Morini et al. 2012). The species accumulation curves indicated a plateau, indicating that the sampling effort was enough to sample the species from localities (Figure 1). Among all sampling areas, the estimated richness is significantly different (Kruskal-Wallis = 57.8806, df = 6, p < 0.01), and average richness ranged from 12 to 15 species (Table 3). The lowest number of species was recorded in locations near the urban area or areas with high human interference, such as urban parks; the urbanized Area I is an exception in this case. The urbanized Area around I is composed of Atlantic Forest vegetation that may be providing increased flow of species among locations. The com- munities’ analysis confirms this observation once there was the formation of two groups (Figures 2 and 3). One composed of areas that are closer to the urban area of the city of Mogi das Cruzes, being directly susceptible to the effects of urbanization, such as Parque Leon Feffer, Sports Center Joana D’Arc, Parque Nagib Najar and urbanized Area II. The other consists of areas that still have Atlantic Forest vegetation or are close to or within the Conservation unit, ie. the urbanized Area I, and Parque Municipal Francisco Affonso de Mello. The areas forming the first group share 21 species while those of the 707 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê Ta bl e 2. R el at iv e fr eq ue nc y of o cc ur re nc e (F R O ) an d ab un da nc e (F R A ) ac co rd in g to th e ta xa a nd s am pl in g si te s lo ca te d in th e ci ty o f M og i d as C ru ze s ( SP ). Su bf am ili es /S pe ci es Sa m pl in g si te s PL F C EC PN J A u I A u II A P PN M FA M FR O FR A FR O FR A FR O FR A FR O FR A FR O FR A FR O FR A FR O FR A D O L IC H O D E R IN A E D or ym yr m ex sp .1 1. 32 0. 35 2. 75 1. 93 1. 72 0. 06 0. 36 0. 56 5. 33 3. 26 0. 35 0. 45 0. 38 0. 01 L in ep ith em a ne ot ro pi cu m W ild , 2 00 7 5. 28 4. 13 3. 44 1. 32 6. 01 1. 84 5. 73 9. 61 5. 33 3. 57 6. 71 18 .2 8 5. 32 3. 94 EC IT O N IN A E L ab id us p ra ed ad or ( Sm ith F ., 18 58 ) 0. 33 0. 01 3. 44 25 .4 2 - - 3. 22 1. 84 - - 2. 12 10 .1 5 2. 66 25 .4 6 L ab id us co ec us ( La tr ei lle , 1 80 2) 0. 33 0. 01 - - - - - - - - - - 0. 38 0. 01 EC T A T O M M IN A E E ct at om m a br un ne um ( Sm ith F ., 18 58 ) - - 2. 75 0. 12 - - 2. 51 0. 67 1. 00 0. 31 0. 35 0. 04 - - E ct at om m a ed en da tu m R og er , 1 86 3 0. 33 0. 01 1. 37 0. 10 0. 43 0. 05 1. 07 0. 24 0. 66 0. 03 - - - - G na m pt og en ys st ri at ul a (M ay r, 18 84 ) 5. 61 3. 49 1. 03 0. 03 2. 15 0. 44 6. 09 9. 90 1. 66 0. 45 4. 24 3. 99 7. 60 12 .2 1 G na m pt og en ys sp .4 - - 0. 69 0. 02 - - - - 0. 33 0. 01 - - - - Th yp ho lo m yr m ex ro ge nh of er i (M aj er , 1 86 2) - - - - - - 0. 36 0. 02 - - - - - - FO R M IC IN A E B ra ch ym yr m ex h ee ri F or el , 1 87 4 0. 66 0. 04 - - 0. 43 0. 01 1. 80 0. 13 0. 33 0. 01 0. 35 0. 01 2. 28 0. 76 B ra ch ym yr m ex p ic tu s M ay r, 18 87 3. 63 0. 82 - - 1. 29 0. 14 0. 72 0. 38 - - 1. 77 0. 16 1. 90 0. 12 B ra ch ym yr m ex in ci su s Fo re l, 19 12 6. 60 7. 07 6. 87 15 .1 6 8. 15 8. 76 5. 01 2. 60 4. 66 3. 35 4. 95 1. 98 3. 04 4. 74 C am po no tu s r ufi pe s ( Fa br ic iu s, 17 75 ) 6. 60 7. 15 6. 18 1. 69 7. 73 2. 97 6. 10 8. 61 4. 33 1. 04 5. 65 3. 08 4. 56 3. 77 708 Sociobiolog y Vol. 59, No. 3, 2012 C am po no tu s ( M yr m ap ha en us ) s p. 2 0. 99 0. 03 2. 06 0. 08 1. 29 0. 08 2. 87 0. 40 2. 00 0. 45 1. 06 0. 10 1. 14 0. 37 C am po no tu s s er ice iv en tr is (G ué ri n- M én ev ill e, 1 83 8) - - 2. 06 0. 49 - - - - - - - - - - C am po no tu s (T ae na m yr m ex ) 0. 66 0. 02 0. 69 0. 10 - - 2. 51 0. 40 0. 66 0. 03 - - 0. 38 0. 03 C am po no tu s ( M yr m ap ha en us ) n ov og ra na de ns is 1. 32 0. 15 0. 69 0. 02 3. 00 0. 20 0. 36 0. 02 2. 00 0. 12 0. 70 0. 04 0. 76 0. 01 C am po no tu s s p. 7 0. 33 0. 01 0. 34 0. 02 - - 0. 36 0. 04 1. 66 0. 18 - - - - C am po no tu s s p. 8 5. 94 1. 53 5. 50 1. 32 3. 43 0. 66 3. 59 0. 69 5. 00 0. 61 2. 83 0. 34 1. 90 0. 31 C am po no tu s s p. 9 0. 33 0. 01 - - - - - - 1. 33 0. 15 - - 0. 38 0. 03 C am po no tu s s p. 10 0. 33 0. 01 - - - - - - - - - - 0. 76 0. 03 C am po no tu s s p. 11 - - 0. 34 0. 02 - - 0. 72 0. 11 2. 66 0. 45 2. 47 0. 21 1. 52 0. 15 C am po no tu s s p. 12 - - - - - - 0. 36 0. 02 - - - - - - C am po nt ot us sp .1 3 - - - - - - - - - - - - 0. 76 0. 08 C am po nt ot us sp .1 6 - - - - 0. 86 0. 03 0. 36 0. 02 - - - - - - C am po nt ot us sp .1 8 - - - - - - - - 0. 33 0. 01 - - - - M yr m el ac hi sta ca th ar in ae M ay r, 18 87 - - - - - - - - - - - - 0. 38 0. 01 M yr m el ac hi sta ru sz ki i F or el , 1 90 3 - - - - - - 0. 36 0. 02 - - 1. 06 0. 05 - - M yr m el ac hi sta sp .4 - - - - - - 0. 36 0. 04 - - - - - - N yl an de ri a fu lv a (M ay r, 18 62 ) 4. 95 4. 27 5. 50 4. 52 8. 58 42 .9 3 0. 72 0. 04 4. 66 7. 98 1. 41 0. 14 2. 66 1. 32 Pa ra tr ec hi na lo ng ico rn is (L at re ill e, 1 80 2) - - - - - - - - 1. 33 0. 11 - - 2. 28 0. 88 M Y R M IC IN A E A cr om yr m ex cr as sis pi nu s ( Fo re l, 19 09 ) 5. 28 0. 58 0. 69 0. 02 - - - - 2. 00 0. 20 0. 71 1. 54 - - A cr om yr m ex d isc ig er M ay r, 18 87 - - 0. 34 0. 01 - - - - 0. 33 0. 01 0. 35 0. 01 - - A pt er os tig m a sp .1 - - 0. 69 0. 02 - - 0. 36 0. 02 0. 33 0. 01 - - - - 709 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê A tta se xd en s (F or el , 1 90 8) - - 3. 09 0. 72 3. 43 0. 97 5. 38 4. 51 - - 2. 12 0. 44 1. 52 0. 39 C ep ha lo te s p us sil us v. b re vi sp in os us - - - - 1. 72 0. 53 0. 72 0. 71 - - - - 0. 38 0. 01 C re m at og as te r ( O rt ho cr em a) sp .1 - - - - - - 0. 36 0. 13 - - - - 1. 90 0. 39 C re m at og as te r s p. 2 0. 33 0. 05 0. 34 0. 01 0. 86 0. 02 2. 15 1. 22 - - - - 0. 76 0. 12 C re m at og as te r s p. 3 0. 66 0. 01 0. 34 0. 10 0. 43 0. 03 - - 2. 66 1. 25 - - - - C re m at og as te r s p. 7 0. 99 1. 01 0. 34 0. 16 1. 29 0. 49 1. 43 0. 44 - - 0. 35 0. 01 - - C yp ho m yr m ex s p. 7 0. 33 0. 01 - - - - - - - - - - - - H yl om yr m a re itt er i ( M ay r, 18 87 ) - - - - - - - - 0. 33 0. 03 - - 0. 38 0. 01 L ep to th or ax sp .1 - - - - - - 0. 36 0. 04 - - - - M eg al om yr m ex sp .4 - - - - - - - - - - 1. 06 0. 05 0. 76 0. 07 M yc et ar ot es p ar al le lu s Em er y, 19 05 - - 0. 69 0. 03 - - - - 0. 33 0. 01 - - - - M yc et os or iti s s p. 1 2. 64 0. 15 0. 34 0. 01 2. 15 0. 11 0. 36 0. 02 1. 00 0. 08 1. 06 0. 12 - - O xy po ec us sp .2 0. 66 0. 07 5. 15 7. 92 - - 0. 72 0. 58 - - 4. 59 11 .8 7 3. 42 6. 33 Ph ei do le s p. 1 - - 2. 75 1. 35 1. 29 0. 02 - - 0. 66 1. 30 2. 47 0. 33 - - Ph ei do le sp .3 1. 98 0. 13 6. 18 3. 40 0. 43 0. 05 3. 22 0. 93 1. 66 0. 52 2. 83 6. 25 0. 76 0. 26 Ph ei do le sp .4 5. 94 23 .1 0 2. 06 1. 97 4. 72 1. 88 2. 87 7. 28 6. 33 29 .1 3 2. 83 3. 08 2. 28 6. 58 Ph ei do le sp .6 0. 66 1. 80 - - 1. 29 0. 09 1. 43 0. 24 3. 66 2. 82 - - 2. 28 1. 14 Ph ei do le s p. 7 0. 33 0. 07 - - 0. 86 0. 87 - - - - - - - - Ph ei do le s p. 9 - - - - 0. 43 0. 31 0. 36 0. 04 - - - - 0. 38 0. 03 Ph ei do le sp .1 3 - - - - - - - - - - 0. 71 0. 09 1. 14 0. 17 Ph ei do le sp .1 4 - - - - - - - - - - 0. 35 0. 08 1. 52 0. 64 Ph ei do le sp .1 5 0. 33 0. 05 - - - - - - - - - - 0. 38 0. 03 710 Sociobiolog y Vol. 59, No. 3, 2012 Ph ei do le s p. 16 - - 3. 43 2. 76 - - - - 0. 33 0. 01 1. 41 0. 07 0. 38 0. 03 Ph ei do le s p. 17 1. 98 1. 49 1. 72 0. 49 3. 00 0. 50 2. 15 6. 19 3. 33 4. 36 0. 35 0. 03 1. 14 0. 65 Ph ei do le s p. 18 - - - - - - - - 0. 18 0. 03 - - - - Ph ei do le s p. 20 0. 66 0. 48 - - - - 0. 36 0. 04 - - 0. 35 0. 11 0. 38 0. 31 Ph ei do le s p. 22 - - 0. 34 0. 08 - - 1. 07 0. 07 0. 22 0. 05 0. 71 0. 27 - - Ph ei do le s p. 23 - - 1. 03 0. 15 - - - - - - 0. 35 0. 40 - - Ph ei do le sp .2 4 1. 98 0. 56 1. 03 0. 18 - - 1. 07 0. 33 1. 00 1. 51 1. 41 2. 85 1. 14 0. 11 Ph ei do le s p. 26 - - - - - - - - - - - - 0. 38 0. 25 Ph ei do le sp .2 8 0. 99 0. 46 0. 34 0. 02 - - - - - - 2. 47 2. 19 1. 90 4. 11 Ph ei do le sp .3 0 1. 32 0. 66 2. 75 2. 06 - - 0. 72 0. 31 - - 3. 89 6. 00 1. 14 4. 30 Ph ei do le sp .3 6 3. 96 4. 88 3. 78 8. 47 2. 15 4. 76 0. 72 0. 84 2. 33 2. 16 2. 83 8. 18 - - Ph ei do le sp .3 8 3. 63 8. 37 2. 75 0. 80 2. 15 0. 92 3. 22 3. 37 3. 66 2. 16 0. 71 0. 14 0. 38 0. 14 Ph ei do le s p. 39 - - - - - - - - - - - - 0. 38 1. 10 Ph ei do le s p. 43 - - - - - - - - 0. 66 0. 99 - - - - Ph ei do le s p. 46 - - - - - - - - 0. 33 0. 04 - - - - Po go no m yr m ex a bd om in al is Sa nt sc hi , 1 92 9 - - - - - - - - 2. 66 0. 69 - - - - Pr oc ry pt oc er us g r. pr . S ch m al zi - - - - - - 0. 36 0. 02 - - - - - - So le no ps is sa ev iss im a (S m ith , 1 85 5) 5. 61 19 .6 4 5. 15 12 .9 5 8. 58 18 .8 6 7. 17 18 .5 2 4. 33 7. 47 4. 95 6. 76 4. 56 8. 23 So le no ps is sp .2 2. 31 0. 66 2. 75 3. 63 4. 29 1. 43 5. 02 5. 26 3. 00 1. 73 3. 53 3. 42 6. 08 5. 10 So le no ps is sp .3 1. 32 2. 15 - - 5. 58 4. 94 0. 72 10 .3 2 1. 00 0. 77 1. 41 2. 05 3. 04 0. 24 St ru m yg en ys sc hm al zi E m er y, 19 06 1. 32 0. 04 - - 0. 43 0. 01 0. 72 0. 04 0. 66 0. 03 - - - - Tr ac hy m yr m ex g r. Se pt en tr io na le s - - - - 0. 35 0. 01 - - 711 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê W as m an ni a sp .3 2. 31 0. 13 1. 03 0. 08 4. 31 4. 63 0. 72 0. 20 3. 00 18 .7 2 2. 12 1. 36 1. 52 0. 39 P O N E R IN A E A no ch et us a lti sq ua m is (M ay r, 18 87 ) - - - - - - 0. 36 0. 02 - - 0. 71 0. 01 0. 38 0. 01 H yp op on er a sp .1 - - 0. 69 0. 02 - - 0. 72 0. 04 - - 0. 35 0. 01 - - H yp op on er a sp .8 0. 33 0. 01 - - - - 0. 36 0. 09 0. 33 0. 06 - - 1. 52 0. 04 H yp op on er a sp .9 - - - - - - - - - - - - 0. 38 0. 03 L ep to ge ny s s p. 3 - - - - - - - - - - - - 0. 76 0. 03 O do nt om ac hu s a ffi ni s G ué ri n- M én ev ill e, 1 84 4 - - 0. 34 0. 01 - - 1. 07 0. 13 - - 1. 06 0. 22 1. 52 0. 15 O do nt om ac hu s m ei ne rt i F or el , 1 90 5 - - - - - - - - - - 0. 71 0. 04 0. 76 0. 03 O do nt om ac hu s c he lif er (L at re ill e, 1 80 2) - - 1. 37 0. 04 0. 43 0. 01 1. 07 0. 11 - - 7. 07 1. 87 6. 08 2. 27 Pa ch yc on dy la st ri at a Sm ith , 1 85 8 6. 60 4. 39 2. 75 0. 15 4. 72 0. 41 6. 09 1. 38 4. 66 1. 11 6. 01 0. 91 6. 84 1. 86 Pa ch yc on dy la h ar pa x (F ab ri ci us , 1 80 4) - - - - 0. 43 0. 01 - - - - - - - - PS Eu D O M Y R M EC IN A E Ps eu do m yr m ex g ra cil is (F ab ri ci us , 1 80 4) - - - - - - 0. 72 0. 09 1. 33 0. 11 1. 77 0. 10 0. 38 0. 07 Ps eu do m yr m ex p al lid us (S m ith , 1 85 5) - - - - - - 0. 36 0. 02 - - - - - - Ps eu do m yr m ex p hy llo ph ilu s ( Sm ith , 1 85 8) - - - - - - - - 2. 00 0. 40 - - - - Pa rt ia l A bu nd an ce 10 .3 05 9. 21 7 8. 36 0 4. 50 4 6. 46 7 7. 25 9 7. 18 8 Pa rt ia l R ic hn es s 44 46 36 56 49 49 56 To ta l A bu nd an ce 53 .3 00 To ta l R ic hn es s 92 Sh an no n D iv er si ty 3. 31 3. 48 3. 21 3. 54 3. 58 3. 50 3. 62 Ev en ne ss 0. 88 0. 91 0. 89 0. 88 0. 92 0. 90 0. 90 712 Sociobiolog y Vol. 59, No. 3, 2012 second group share 32 species. The high exchange rate of species between areas near the Atlantic Forest suggests the importance of preserving these sites for conservation of the regional ant fauna in the Serra do Itapeti and at the same time, they can act as buffer areas for the Conservation unit. Taxa with specialized morpholog y and biolog y (Brandão et al. 2009) were observed in the areas under the influence of the Atlantic Forest, such Table 3. Average number of species per sample and richness estimates of epigaeic ants collected around the city of Mogi das Cruzes (SP). Area Average number of species (± sd) Observed number of species Richness estimate PLF 15 (±1.84) 44 51.46 CEC 15 (±3.78) 46 51.34 PNJ 12 (±2.94) 36 42.65 AuI 14 (±3.05) 56 67.28 AuII 15 (±2.25) 49 59.45 AP 14 (±3.30) 49 55.53 PNMFAM 13 (±4.51) 56 70.36 Fig. 1. Accumulation curves of epigaeic ant species sampled around the city of Mogi das Cruzes (SP). ( ) PLF; ( ) CEC; ( ) AuI; ( ) PNJ; ( ) AuII; ( ) AP; ( ) PNMFAM. 713 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê as the urbanized Area I, Private Area and the Parque Natural Municipal Francisco Affonso de Mello; especially Ectatomminae and Ponerinae species. Myrmelachista, which is exclusively arboreal (Longino 2006) since most of its species nests in cavities of tree trunks and branches of living trees (Stout 1979; Brown 2000; Longino 2006; Edwards et al. 2009), was found only in sites in contact with forest areas. Only one exotic species, (Paratrechina longicornis Latreille) was recorded, including in the Conservation unit. This species and P. megacephala Fabricius are present in the central region of Mogi das Fig. 2. Non-metric multidimensional scaling (NMDS) comparing epigaeic ant communities sampled around the city of Mogi das Cruzes (SP). (Stress = 27.71) - ( ) PLF; ( ) CEC; ( ) PNJ ( ) AuI; ( ) AuII; ( ) AP; ( ) PNMFAM. 714 Sociobiolog y Vol. 59, No. 3, 2012 Cruzes and neighborhoods built in the vicinity of the Serra do Itapeti (Kamura et al. 2007; Munhae et al. 2009; Souza et al. 2012). However, exhaustive inventories of litter in places sheltered from the Serra do Itapeti have registered exotic spe- cies (Morini et al. 2012; Suguituru et al. in preparation). The absence of these species is important for maintaining the biodiversity of Serra do Itapeti since they have potential to reduce native fauna (Nafus 1993; Harris & Barker 2007; Vanderwoude et al. 2000; Hoffmann 2010). However, it is necessary to monitor the populations of P. longicornis, as the species is already in areas of the Parque Natural Municipal Francisco Affonso de Mello where population’s visit is constant. This conservation area is the largest one of Serra do Itapeti under legal protection (Law No. 6220 of 29.12.2008), being very rich in fauna and flora (Morini & Miranda, 2012), although located in the metropolitan area of São Paulo city. ACKNOWLEDGEMENTS The authors would like to thank the CNPq for the scholarships granted to the first two authors (grants 432.484/2008 and 100479/2009-5) and MSCMorini (grant 301151/2009-1) and FAPESP for for financial support (grant 06/52409-6). REFERENCES Alonso, L.E. & D. Agosti 2000. Biodiversity studies, monitoring and ants: a overview. p. 1-8. In: Agosti, D., Majer, J.D., Alonso, L.E. & T.R. Shultz (eds.). Ants – Standard methods for measuring and monitoring biodiversity. Washington, DC: Smithsonian Institution Press, Washington. Arcila, A.M.C. & F.H. Lozano- Zambrano 2003. Hormigas como herramienta para la bioindicación y el monitoreo. p. 159- 166. In: Fernández, F. (org.). Introdución a las Fig. 3. Dendrogram of Bray-Curtis dissimilarity based on the composition of epigaeic ant species collected around the city of Mogi das Cruzes (SP). 715 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê hormigas de la Región Neotropical. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, Colombia. Ayres, M., Ayres, Jr. M., Ayres, D.L. & A.S. Santos 2007. BioEstat 5.0: aplicações estatísticas nas áreas de Ciências Biológicas e médicas. Instituto do Desenvolvimento Sustentável Mamirauá. IDSM/MCT/CNPq, 364p. Baccaro, F.B., Ketelhut, S.M. & J.W. Morais 2011. Efeitos da distância entre iscas nas estimativas de abundância e riqueza de formigas em uma floresta de terra-firme na Amazônia Central. Acta Amazon. 41: 115-122. Baroni-urbani, C. & M.L De Andrade 2007. The ant tribe Dacetine: limits and constituent genera, with descriptions of new species. Ann. Mus. Civ. Stor. Nat. Giacomo Doria 99:1-191. Bolton, B.A. 1994. New general catalogue of ants of the world. Harvard university Press. 222, p. Bolton, B.A. 2003. Synopsis and classification of Formicidae. Memoirs of the American Museum Entomological Institute 71: 1-370. Brandão, C.R.F., Silva, R.R. & J.H.C. Delabie 2009. Formigas (Hymenoptera). p. 323-369. In: Panizzi, A.R. & J.R.P. Parra (eds). Bioecologia e Nutrição de Insetos: base para o manejo integrado de pragas. Brasília, DF, Embrapa Tecnológica, 1,164p. Brown Jr, W.L. 2000. Diversity of ants. p. 45-79. In: Agosti, D.; Majer, J.D.; Alonso, L.E. & T.R.Schultz (eds). Ants: standard methods for measuring and monitoring biodiversity. Washington: Smithsonian Institution Press. Candido, M. S., Fonseca, A. C. R. & V.M. Dantas. 2010 Protocolo em defesa da recuperação da qualidade socioambiental da Bacia Hidrográfica do Alto Tietê Cabeceiras. 94p. Colwell, R.K. 2009. EstimateS: statistical estimation of species richness and shared species from samples. Version 8.2. http://viceroy.ceb.uconn.ed/EstimateS Edwards, D.P., Frederickson, M.E., Shepard, G.H. & D.W.Yu 2009. A plant needs ants like a dog needs fleas: Myrmelachista schumanni ants gall many trees species to create housing. Am. Nat. 174:734–740. Folgarait, P. J. 1998. Ant biodiversity and its relationship to ecosystem functioning : a review. Biodiversity Conservation 7:1221-1244. Harris, R.J. & G. Barker 2007. Relative risk of invasive ants (Hymenoptera: Formicidae) establishing in New Zealand. New Zealand Journal of Zoolog y 34:161-178. Hoffmann, B.D. 2010. Ecological restoration following the local eradication of an invasive ant in Northern Australia. Biological Invasions 12:959-969. Holway, D.A., Lach, L., Suarez, A.V., Tsutsui, N.D. & T.J. Case 2002. The causes and consequences of ant invasions. Annual of Review of Ecolog y and Systematics 3:181- 233. Hölldobler, B. & E.O. Wilson 1990. The Ants. Harvard university Press, Cambridge, 732 p. Kamura, C.M., Morini, M.S.C., Figueiredo, C.J., Bueno, O.C. & A.R.C. Campos-Farinha 2007. Ant communities (Hymenoptera: Formicidae) in an urban ecosystem near the Atlantic Rainforest. Brazil. J. Biol. 67: 635-641. 716 Sociobiolog y Vol. 59, No. 3, 2012 Lapolla, J., Brady, S. & S. Shattuck 2010. Phylogeny and taxonomy of the Prenolepis genus group of ants (Hymenoptera: Formicidae). Syst. Entomol. 35:118-131. Longino, J.T. 2006. A taxonomic review of the genus Myrmelachista (Hymenoptera: Formicidae) in Costa Rica. Zootaxa 1141:1–54. López-Moreno, I. R., Diaz-Betancourt, M.E. & T.S. Landa 2003. Insectos Sociales em Ambientes Antropizados: Las Hormigas de la Ciudad de Coatepec, Veracruz, México. Sociobiolog y 42:605-622. Mcintyre, N. E., Rango, J., Fagan, W. F. & S.H. Faeth 2001. Ground arthropod community structure in a heterogeneous urban environment. Landsc. and urban plan. 52: 257- 274. Minuzzi, R.B., Sediyama, G.C., Barbosa, E.M. & J.C.F. Melo Jr. 2007. Climatologia do comportamento do período chuvoso da região sudeste do Brasil. Rev. Bras. de Meteorol. 22:338–344. Morini, M.S.C. & V. Miranda 2012. Serra do Itapeti: aspectos históricos, sociais e naturalísticos. Editora Canal 6. Morini, M.S.C., Silva, R.R.S., Suguituru, Pacheco, R. & M.A Nakano 2012. Formigas da Serra do Itapeti. p. 195-213. In: M.S.C.Morini & V.F.O.Miranda (org.). Serra do Itapeti: aspectos históricos, sociais e naturalísticos. Editora Canal 6. Munhae, C.B., Bueno, Z.A.F.N., Morini, M.S.C. & R.R. Silva 2009. Composition of the Ant Fauna (Hymenoptera: Formicidae) in Public Squares in Southern Brazil. Sociobiolog y 53: 455-472. Murphy, D. D. 1988. Desafios à diversidade biológica em áreas urbanas. p. 89-97. In: Wilson, E.O.(ed.). Biodiversidade. Rio de Janeiro: Ed. Nova Fronteira. Nafus, D.M. 1993. Movement of introduced biological control agents onto nontarget butterflies, Hypolimnas spp. (Lepidoptera: Nymphalidae). Environ. Entomol. 22:265- 272. Oksanen, J., Kindt, R., Legendre, P., O’hara, B., Simpson, G,L., Solymos, P., Stevens, M.H.H. & H. Wagner 2009. Vegan: Community ecolog y package. R package version 1.15-1. http://cran.rproject. org/, http://r-forge.r-project.org/projects/vegan/ Oliveira, M. F. de & A.E de C. Campos-Farinha 2005. Formigas urbanas do município de Maringá, PR, e suas implicações. Arq. Inst. Biol. 72: 33-39. Rodrigues, J. J. S.; Brown Jr., K. S. & A. Ruszczyk 1993. Resources and conservation of neotropical butterflies in an urban forest fragments. Biol. Conserv. 64: 3-9. Rodrigues, W.C. 2007. DivEs: Diversidade de espécies Version 2.0. www.ebras.bio.br/ dives. Sanders, D. & Van F.F. Veen 2011. Ecosystem engineering and predation: the multi-trophic impact of two ant species. J. Anim. Ecol. 80: 569-576. Savard, J.P.L., Clergeau, P. & G. Mennechez. 2000. Biodiversity concepts and urban ecosystems. Landsc. urban Plan. 48: 131-142. 717 de Souza, D.R. — Diversity of Epigeal Ants in Alto Tietê Schultz, T.R. & T.P. McGlynn 2000. The interactions of ants with other organisms, p. 35-44. In: Agosti, D., Majer, J.D., Alonso, L.E. & T.R. Schultz (eds.). Ants: standard methods for measuring and monitoring biodiversity. Smithsonian Institution Press,Washington. Scott, J.M., Csuti, B. Jacobi, J.D. & J.E. Estes 1987. Species richness. A geographical approach to protecting future biological diversity. Biosci. 37: 782-788. Souza, D.R., Munhae, C.B., Kamura, C.M., Portero, N.S. & M.S.C. Morini 2012. Formigas em áreas urbanizadas da Serra do Itapeti. p. 296-304. In: Serra do Itapeti: aspectos históricos, sociais e naturalísticos (M.S.C.Morini and V.F.O.Miranda org.). Stout, J. 1979. An association of an ant, a mealy bug, and an understory tree from a Costa Rican Rain Forest. Biotropica 11:309–311. Suguituru, S.S., Souza, D.R., Munhae, C.B., Pacheco, R. & M.S.C. Morini. Diversidade de formigas (Hymenoptera: Formicidae) em remanescentes de Mata Atlântica na Bacia Hidrográfica do Alto Tietê. (em preparação). Vanderwoude, C., Lobryn De Bruyn, L.A. & A.P.N. House 2000. Response of an open forest community to invasion by the introduced ant, Pheidole megacephala. Austral Ecolog y 25:253-259. Ward, P.S. 2000. Broad-scale patterns of diversity in leaf litter ant communities. p. 99-121. In: n: Agosti, D., Majer, J.D., Alonso, L.E., Schultz, T. (eds.). Ants: standard methods for measuring and monitoring biodiversity. Smithsonian Institution Press, Washington. Yamaguchi, T. 2004. Influence of urbanization on ant distribution in parks of Tokyo and Chiba City, Japan, I. Analysis of ant species richness. Entomol. Sci. 19:209-216. Yamaguchi, T. 2005. Influence of urbanization on ant distribution in parks of Tokyo and Chiba City, Japan, II. Analysis of species. Entomol. Sci. 8:17-25.