CLINICAL GUIDELINE Practical radiology of plasmacytoma and multiple myeloma Andrew du Toit MB ChB, FFRad Lucille Wood BA (Nurs Sci), MSc (Medicine) (Haematol), RN, RM, Dip. Intensive Nursing Care, Ward Admin and Clinical Teaching Peter Jacobs MBBCh, MD, PhD (Medicine), FCP (SA), FACp, FRCp, MCAp, FRC Path, FRS Department of Haematology and Bone Marrow Transplant Unit incorporating the Searl/ Research Laboratory for Cel/ular and Molecular Biology, Constantiaberg Medi·C/inic, Cape Town Abstract The traditional approach to diagnosis and staging in myeloma, based on haematologie and biochemical crite- ria, can be improved by inclusion of new forms of imaging. Standard radi- ographs have limited value but are still required because the majority of patients present with disease readily detected by this means. Also rapid extensive skeletal coverage is possible. Scintigraphy is limited by its poor sen- sitivity. CT is restricted to a region of interest but is however more accurate than DXA for demonstrating trabecu- lar bone loss and density so that it emerges as valuable in the evaluation of therapy. MR! is currently the best choice for revealing marrow infiltra- tion and, in ideal circumstances, the most sensible combination includes spine, pelvis and proximal femur MR! with skull, chest and rib X-rays. Furthermore MR! is finding a place in documenting prognosis when these individuals are treated. Introduction Localised or disseminated mono- clonal proliferation of plasma cells produces a wide range of symptoms and signs primarily in the skeleton. Prominent among the latter is dis- comfort due to generalised osteo- porosis, solitary or multiple lytic lesions I in addition to pathological fracture.' Associated is synthesis of a paraprotein which can typically be detected in serum and as Bence-lones proteins in urine although the more primitive variants may not secrete and occasionally not even produce the aberrant immunoglobulin molecule.' Metabolic consequences are increases in blood calcium and urate that are risk factors for kidney failure.' Invasion of the bone marrow impairs haematopoiesis' with both humoral and cell-mediated immunity being compromised," The cause is unknown although the natural history is well described with median survival of only 7 months.' In contrast modern 22 SA JOURNAL OF RADIOLOGY • February 2003 management improves quality of life by optimum support and combina- tions of alkylating agents with corti- costeroids while investigational approaches centre on haematopoietic stem cell transplantation and thalido- ruide." Clinical features Presentation may be protean and with the increasing use of routine bio- chemical screening, as part of health care management, diagnosis can be made when only biochemical changes are reported incidentally from the lab- oratory. Prominently pain is axial reflecting demineralisation that may be severe, is often aggravated at night and is char- acteristically responsive to biphospho- nates." Areas of destruction vary in number and size. Strong correlations exist with cytokines that alter both osteoclastic and osteoblastic activity.II Myelomatous masses may erode the cortex and give rise to large subcuta- neous swellings, underlie breaks, pre- dispose to vertebral compression and occasionally present as tumours in breast, stomach or adjoining the spinal column. Haematologically there are vary- ing degrees of normochromic and normocytic anaemia pathophysiolog- ically attributed to the chronic inflam- matory state and associated with a marked increase in erythrocyte sedi- mentation rate. Until late in the course leucocyte and platelet counts are pre- served." Bone marrow aspiration reveals increased numbers of plasma- cytes many of which may be pleomor- phic and, together with their extent and distribution seen in the trephine, are included in some staging proce- dures." Biochemical consequences are CLINICAL GUIDELINE increases in calcium and urate with a distinctive spike in the gamma region on serum protein electrophoresis. Free light chains, filtered at the glomerulus, are then deposited in the tubules so contributing to renal dys- function with polyuria and dehydra- tion culminating in a self-perpetuat- ing cyde that is, however, initially reversible. Sometimes profound degrees of immune compromise reduce poly- donal antibodies and predispose to sinopulmonary infection. Approxi- mately 10% of patients develop amy- loidosis that has widespread effects typically producing nephrotic syn- drome but perhaps more ominously restrictive cardiomyopathy. A wide range of other features exist to trap the unwary diagnostician and a high degree of awareness will often lead to an early diagnosis." Laboratory evaluation There is no substitute for a careful- ly taken history followed by meticu- lous examination, maintaining a high degree of awareness and simple bed- side tests of which urinalysis remains invaluable." The next stage is confirmation and since a number of the findings are mimicked by other disorders it is wise to combine as many abnormal find- ings as possible to secure the diagnosis but primarily rely on the presence of the serum paraprotein designated an M-peak and intramedullary plasma- cytosis. Staging defines anticipated out- come and provides a practical means for monitoring management. The most widely used of these is that described by Durie and Salmon (Table I)." Recent studies support the use of B2 microglobulin and C-reac- tive protein." An approach to imaging Plain film radiography Approximately 80% of individuals have abnormalities at presentation and this remains a logical first step in studying the skeleton." However no systematic approach appears to have been described for the integration of these changes into established staging systems":" although there is, not sur- prisingly, a loose correlation implicit between advancing tumour and skele- tal damage. To provide a more struc- tured approach to this risk factor a new grading is proposed (Table II). Bone mineral density As part of an ongoing evaluation, (Jacobs, Wood, Hitchcock and du Toit - unpublished data) this is quanti- tated initially and followed serially seeking to define the influence of dif- ferent interventions, particularly the use of biphosphonates." We have found that the much favoured DXA often underestimates trabecular thin- ning which is readily evident on com- puterised axial tomography." Scintigraphy Radionuclide scanning using tech- netium-99m labelled diphosphonate is typically normal or shows areas of decreased uptake. The explanation for this relative insensitivity is that the osteoblastic activity in these tumours is outweighed by the aggressive Stage Criteria TableI.Classificationof myeloma (modified from Durie and Salmon'S). The best predictors are degree of anaemia, quantitation of paraprotein and serum calcium level I: All must be present II III: One or more only needed Subclassification A:= Serum creatinine < 120 lJmol/l il= Serurn creatinine> 120 IJ.mol/l. 23 SA JOURNAL OF RADIOLOGY • February2003 • Haemoglobin value> 100 gil • Serum calcium < 2.2 mmol/l • Radiographically Normal bone structure Solitary bone plasmacytoma •M-component IgG < 50 gil 19A< 3 gil Urine light chain < 4 gl24 hours Fitting neither Stage I nor Stage III • Haemoglobin value < 85 gil • Serum calcium value >2.2 mrnol/l • Advanced osseous destruction • M-component IgG > 70 gil 19A> 50 gil Urine light chain> 12 gl24 hours CLINICAL GUIDELINE Table II. Constantiaberg grading of skeletal changes (Jacobs, Wood and du Toit- unpublished data). Uncertainty exists as to whether the total amount of cortical damage reflected in multiple small defects has different value to a smaller number of much larger areas of destruction Grade Criteria o I II a. b. c. III N Normal Diffuse demineraIisation Less than 10 lytic lesions Maximum 1 cm 2-4cm Greater than 5 cm More than 10 bony defects Associated pathological fracture destruction of the hypertrophic osteo- clasts." Computerised tomography (CT) Although not routinely used it may nevertheless demonstrate early trabecular bone destruction within a vertebral body where other imaging modalities are negative. Magnetic resonance imag- ing(MRI) Support derives from a greater sensitivity in revealing otherwise occult invasion which is seen in about 50% of asymptomatic patients with normal Xvrays." Representative areas are full spine, pelvis and proximal femora because these contain the nor- mal haematopoietic tissue that har- bours the neoplasm. Three patterns are discern able (Table III).21 Focal accumulations exhibit low- Tl and low or high T2 signal in approximate- ly equal numbers of untreated cases. The variegated or salt and pepper appearance has small nodules on Tl and is sometimes best appreciated in the pelvis. A diffuse involvement has similar intensity to the isolated deposits with homogenous replace- ment resembling other intra- medullary proliferative disorders. Mixed pictures occur depending on the status of the immunoproliferative neoplasm. This variable appearance results from relatively even neoplastic distribution throughout the haematopoietic tissue in widespread disease contrasting with the small or large nodules composed entirely of tumour in localised patterns. Com- pounding factors are increasing age with conversion of red to yellow mar- row that influences both detection and character of the image produced by the myeloma. Thus, although nor- Table m. Magnetic resonance imaging in myeloma (modified from Kaplan et al.21) Signal Pattern Tl T2 Focal Low High or low Variegated or salt & pepper Low Mild high Diffuse Low High or low 24 SAJOURNAL OF RADIOLOGY. February2003 mal haematopoiesis is impaired with replacement of the former, the relative increase in the adipocytes gives an enhanced signal, which offsets reduc- tion from areas of infiltration, and so the scan may look normal. Contrast enhancement with gadolinium is often marked but neither specific nor essential if any abnormality has already been demonstrated. Similarly medullary invasion may be patchy although widespread overall so that an adequate trephine biopsy will invariably demonstrate the aetiology. Hence MRI is not usually required to direct the aspiration site. Vertebral fractures are seen with significant osteoporosis, which is fre- quent, and approximately 60% of compression fractures look benign. Thus in 37 patients with 224 fractures, 67% had this appearance, 33% appeared malignant and, interestingly, 14 of these or 38% had no sinister fea- tures." The distribution of such radi- ological changes was similar to that observed in non-myelomatous dem- ineralisation predominantly in the lower dorsal and upper lumbar regions. In contrast ominous features are low Tl signal extending from the vertebral body into the pedicles and posterior elements, diffuse body involvement, multiple level involve- ment and extension outside of bone to form a soft tissue mass." The anatomical site may be helpful in detecting malignancy since those above T4 are usually pathological. A prognosis on therapeutic out- come can be correlated with Durie and Salmon stage III where four groups are defined; normal, fewer or more than 10 focal sites and wide- spread infiltration." It is notable that with apparently solitary lesions MRI will typically reveal other deposits. CLINICAL GUIDELINE Following therapy those with lower grades based on both biopsy and radi- ological criteria experienced signifi- cantly longer fracture-free survival than where more extensive changes were present. It is also useful to forecast response. In Durie and Salmon stage I invasion is associated with subsequent progression." Stage III patients with normal images (24%) achieve better results with therapy and survive longer than those where this is abnor- mal." When coupled with CRP this pair were the best independent prog- nostic indicators for survival. This method can be refined using contrast when complete response is defined as resolution of the baseline abnormality and is superior to persistence of any change even if this is only peripheral rim enhancement." References 1. Ravaud p, Thepot C, Auleley GR, Amor B. Imaging of multiple myeloma. Ann Med Interne 1996; 147: 370-375. 2. Scutellari PN, Orzincolo C. Bone disease in mul- tiple myeloma: analysis of 253 controlled cases, with reappraisal of diagnostic criteria and cur- rent imaging techniques. Radiol Med 1993; 85: 235-246. 3. Bartoloni C, Flamini G, Logroscino C, et al. IgD (kappa) 'nonsecretory' multiple myeloma: report of a case. Blood 1980; 56: 898-901. 4. Clark AD, Shetty A, Soutar R. Renal failure and multiple myeloma: pathogenesis and treatment of renal failure and management of underlying myeloma. Blood Rev 1999; 13: 79-90. 5. Rajkumar SV; Fonseca R, Dewald Gw, et al. Cytogenetic abnormalities correlate with the plasma cell labeling index and extent of bone marrow involvement in myeloma. Cancer Genet Cytogenet 1999; 113: 73-77. 6. Paglieroni T, MacKenzie MR. Studies on the pathogenesis of an immune defect in multiple myeloma. J Clin Invest 1977; 59: 1120-1133. 7. Bataille R. Multiple myeloma. N Engl J Med 1997; 336: 1657-1664. 8. Huff CA, Jones RJ. Bone marrow transplanta- tion for multiple myeloma: where we are today. Curr Opin Oneo12002; 14: 147-151. 9. Tosi P, Cavo M. Thalidomide in multiple myelo- ma: state of art. Haematologica 2002; 87: 233- 234. 10. Berenson JR. Bone disease in myeloma. Current Treatment Options in Oncology 200 I; 2: 271-283. 11. Ely SA,Knowles OM. Expression of CD56/neur- al cell adhesion molecule correlates with the presence of lytic bone lesions in multiple myelo- ma and distinguishes myeloma from monoclon- al gammopathy of undetermined significance and lymphomas with plasmacytoid differentia- tion. Am J Patho12002; 160: 1293-1299. 12. Jacobs P, Wood L. Myeloma and other immuno- proliferative disorders. CME 1999; 17: 253-257. 13. Bartl R, Frisch B. Bone marrow histology in multiple myeloma: prognostic relevance of his- tologic characteristics. Hematol Rev 1989; 3: 87- 108. 14. Jacobs P. Myeloma. Disease-a-Month 1990; 36: 317-371. 15. Durie BG, Salmon SE. A clinical staging system for multiple myeloma: correlation of measured myeloma cell mass with presenting clinical fea- tures, response to treatment, and survival. Cancer 1975; 36: 842-854. 16. The Myeloma Trialists' Collaborative Group. Combination chemotherapy versus melphalan plus prednisone as treatment for multiple myeloma: an overview of 6,633 patients from 27 randomised trials. J Clin Oneo11998; 16: 3832- 3842. 17. Collins C. Multiple myeloma. Tn:Husband JES, Reznek RH, eds. Imaging in Oncology. New York: ISIS Medical Media, 1998: 625-634. 18. Berenson JR, Lichtenstein A, Porter L, et al. Long-term pamidronate treatment of advanced multiple myeloma patients reduces skeletal events. The Myeloma Aredia Study Group. J Clin Oneal 1998; 16: 593-602. 19. Solomon 0, Jacobs P.Bone densitometry- role of quantitative computed tomography. S Afr Med J2oo2; 92: 486. 20. Abildgaard N, Glerup H, Rungby J, et al. Biochemical markers of bone metabolism reflect osteoclastic and osteoblastic activity in multiple myeloma. Eur J Haematol2oo0; 64: 121-129. 21. Kaplan PA, Helms CA, Dussault R, Anderson MW, Major NM. Musculoskeletal MRT. Philadelphia: Saunders, 2001: 23-53. 22. Lecouvet FE, Van de Berg BC, Maldague BE, et al. Vertebral compression fractures in multiple myeloma. Part 1. Distribution and appearance at MR imaging. Radiology 1997; 204: 195-199. 23. Lecouvet FE, Malghem J, Michaux L, et al. Vertebral compression fractures in multiple myeloma. Part U. Assessment of fracture risk with MR imaging of spinal bone marrow. Radiology 1997; 204: 201-205. 24. Lecouvet FE, Van de Berg BC, Michaux L, et al. Stage TIJ multiple myeloma. clinical and prog- nostic value of spinal bone marrow MR imag- ing. Radiology 1998; 209: 653-660. 25. Van de Berg BC, Lecouvet FE, Michaux L, et al. Stage I multiple myeloma: value of MR imaging of the bone marrow in the determination of prognosis. Radiology 1996; 201: 243-246. 26. Moulopoulos LA, Dimopoulos MA, Alexanian R, Leeds NE, Libshitz HI. Multiple myeloma: MR patterns of response to treatment. Radiology 1994; 193: 441-446. 25 SA JOURNAL OF RADIOLOGY • February 2003