Sudan Journal of Medical Sciences Volume 12, Issue no. 1, DOI 10.18502/sjms.v12i1.856 Production and Hosting by Knowledge E Research Article The Initial Experience of Trans-Rectal Ultrasound and Biopsy in Diagnosis of Carcinoma Prostate in Gezira Hospital for Renal Disease and Surgery (GHRDS) Walaa Eldin Ibraheem1, Sami Mahjoub Taha2, Mustafa Omran Mansour2, and Mohammed El Imam Mohamed Ahmed2 1Department of Surgery, Faculty of Medicine, Assistant professor, University of West Kordofan 2Department of Surgery, Faculty of Medicine, University of Gezira & Department of Urology, Gezira Hospital for Renal Disease and Surgery, Medani, Sudan Abstract Background: Prostate cancer prevalent cancer in males above sixty-five worldwide, this lead to the introduction of screening of the PSA and using of the transrectal ultrasound scanning, and sextant biopsy of the prostate. Objectives: To compare the accuracy of the Transrectal Ultrasound guided biopsy (TRUS/BX) in the diagnosis of prostate cancer in Gezira Hospital for Renal Diseases and Surgery (GHRDS), with specific considerations to the digital rectal examination (DRE) findings and prostate specific antigen (PSA) level. Materials and Methods: This is a prospective, descriptive small-scale hospital based study. A total of 297 patients with clinically symptomatic enlarged prostate underwent transrectal ultrasound guided true cut needle biopsy of the prostate were studied in (GHRDS) in the period from June2006 to June2009. Results: The majority 188 (63.3%) of patients were between 50-70 years of age. Abnormal digital rectal examination (DRE) like obliteration of the median sulcus, and fixed mucosa revealed higher incidence of carcinoma prostate (CaP) with a significant value (p = 0.0000). PSA level showed significant relation (p = 0.0001) with the diagnosis of carcinoma prostate. Transrectal U/S findings well correlated to the histopathological results, where abnormal findings (like hypo-echoic lesions or calcifications and cysts) showed higher incidence of malignancy in 46 patients constitute 52.8% of the abnormal U/S findings. Conclusions and recommendations: PSA level is highly sensitive but less specific in detection of prostate cancer. Normal DRE doesn’t exclude prostate cancer, fixed mucosa and obliterated median sulcus has the highest predictors of cancer prostate in DRE. Presence of calcifications and cyst on trans-rectal ultrasound has the highest liability for cancer prostate in compare to the other ultrasonic findings. Keywords: Prostate cancer, DRE, PSA level, TRUS/ BX (Transrectal ultra sound biopsy), sextant biopsy How to cite this article: Walaa Eldin Ibraheem, Sami Mahjoub Taha, Mustafa Omran Mansour, Mohammed El Imam Mohamed Ahmed, (2017) “The Initial Experience of Trans-Rectal Ultrasound and Biopsy in Diagnosis of Carcinoma Prostate in Gezira Hospital for Renal Disease and Surgery (GHRDS),” Sudan Journal of Medical Sciences, vol. 12 (2017), issue no. 1, 25–32. DOI 10.18502/sjms.v12i1.856 Page 25 Corresponding Author: Sami Mahjoub Taha; email: samimahj@gmail.com Received: 15 December 2016 Accepted: 20 February 2017 Published: 28 May 2017 Production and Hosting by Knowledge E Walaa Eldin Ibraheem et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Editor-in-Chief: Prof. Mohammad A. M. Ibnouf http://www.knowledgee.com mailto:samimahj@gmail.com https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Sudan Journal of Medical Sciences Production and Hosting by Knowledge E الخلفية: سرطان البروستات هو سرطان منتشر يف الذكور فوق سن الخامسة والستين يف جميع أنحاء العالم، ولهذا السبب تم إدخال فحص ىب أس أىي وإستخدام المسح بالموجات فوق الصوتية عبر المستقيم، وخزعة سيكسانت من البروستات. عبر الموجهة الصوتية فوق بالموجات المأخوذة الخزعة دقة مقارنة هى األهداف: المستقيم يف تشخيص سرطان البروستات يف مستشفى الجزيرة ألمراض وجراحة الكلى، مع إعتبارات محددة لنتائج فحص المستقيم األصبعى ومستويات فحص ىب أس أىي. ما درس وقد بالمستشفى قائمة صغير نطاق على وصفية إستباقية دراسة ىف الطرق: مجموعه ٢٩٧ مريضاً مع أعراض سريرية وتضخم للبروستات الموجات فوق الصوتية عبر المستقيم موجهة خياطة إبرة قطع حقيقية من البروستاتا يف (غردز) يف الفترة من يونيو ٢٠٠٦ إىل يونيو ٢٠٠٩. فحص العمر. من سنة ٥٠-٧٠ بين المرضى من (٪٦٣٫٣) ١٨٨ األغلبية كانت النتائج: المخاطي والغشاء األوسط، التلم من طمس مثل طبيعي غير األصبعى المستقيم .(٠٫٠٠٠٠ = p) الماتصق كشفت عن حدوث أعلى من سرطان البروستات مع قيمة كبيرة البروستات. سرطان تشخيص مع (٠٫٠٠٠١ = p) كبيرة عالقة أىي أس ىب مستوى أظهر النتائج مع جيد بشكل المستقيم عبر الموجهة الصوتية فوق الموجات نتائج وترتبط النسيجية، حيث أظهرت نتائج غير الطبيعية (مثل األورام أو التكلسات واألكياس) ارتفاع الصوتية فوق الموجات نتائج من (٪٥٢٫٨) شكلت حيث مريضا ٤٦ يف الخباثة نسبة الموجهة عبر المستقيم الغير طبيعية. عن الكشف يف تحديداً أقل ولكن للغاية حساس أىي أس البى مستوى اإلستنتاجات: سرطان وجود يستبعد ال الطبيعى األصبعى المستقيم فحص البروستات. سرطان من تنبؤات أعلى لديهما التلم وسط وطمس المثبت المخاطي والغشاء البروستات، سرطان البروستات يف نتائج فحص المستقيم األصبعى . وجود التكلسات والكيسات على الموجات فوق الصوتية عبر المستقيم لديه أعلى مسؤولية عن سرطان البروستات مقارنة للنتائج األخرى من الموجات فوق الصوتية. 1. Introduction Prostate cancer is more common in countries with higher proportions of elderly men in their population, and so accounts for around 15% of cancers in men in developed countries, but only 4% in developing countries [1]. In Sudan prostate cancer was not considered among the top 10 commonest cancer in Sudan in the period (1967-1984), but in the period (1984-2004), it was listed at the bottom of the top 10 common male cancers [2]. Prostate cancer in Gezira state, Sudan, was studied in patients diagnosed and treated in the National Cancer Institute & GHRDS in Wad Medani in the period 2002-2007, comprised 8% of all cancer in the period before TRUS introduction. It jumped to 14% in the first year after introduction of TRUS/BX. The prostate cancer became number one DOI 10.18502/sjms.v12i1.856 Page 26 Sudan Journal of Medical Sciences Production and Hosting by Knowledge E male cancer compared to number three cancers before TRUS was introduced. However, no other study was reported in Sudan regarding TRUS/BX [2]. Before TRUS improvements and serum PSA testing became widespread, clinicians relied mainly on digital rectal examination to establish a diagnosis of prostate cancer and performed digitally directed lesion biopsies. The presence of focal nodules on digital rectal examination still will prompt a biopsy using the TRUS technique regardless of PSA levels. TRUS-directed prostate needle biopsy remains the gold standard for diagnosis of prostate cancer [3]. The risk of a positive DRE turning out to be cancer is dependent greatly on the PSA value. This was shown by the nomogram, which showed an increase in the positive predictive value as the PSA level increase from 0 to 10 ng/ml [4]. PSA-based screening programs have markedly improved early prostate cancer detection. These initiatives were significantly increase the rate of organ-confined and potentially curable disease [6]. Currently, most clinicians recommend biopsy once a patient’s serum PSA rises above 4.0 ng/mL [4]. Evidence for lowering the PSA threshold from work by Catalona’s group showed higher rates of organ-confined disease at the time of radical retropubic prostatectomy in men sampled with PSAs in the 2.6- to 4.0-ng/mL range [7]. These findings have led many urologists to recommend prostate biopsy to men younger than 60 years of age once their PSA level rises above 2.5 ng/mL [4]. There is a general trend toward allowing older men (70 years or older) to have slightly higher “normal” PSAs, in the range of 5.5 to 6.5 ng/mL, although this is not universally accepted [8, 9]. Patients with a serum PSA value between 4.0 and 10.0 ng/mL, using a percentage of free PSA thresholds of less than 25% detected 95% of cancers while eliminating 20% unnecessary biopsies, and within this group, the risk of prostate cancer increased dramatically as the percentage of free PSA level declined [10]. Regardless of initial PSA value, a PSA velocity greater than 0.75 to 1.0 ng/mL per year is frequently associated with prostate cancer and warrants biopsy [9], whereas an elevated PSA density and PSA density-Transitional zone have both been shown to increase the likelihood of diagnosing prostate cancer on repeat biopsy [11]. The original sextant biopsy scheme, (one core from the base, mid, and apex bilaterally) significantly improved cancer detection over digitally directed biopsy of palpable nodules and ultrasound-guided biopsy of specific hypo- echoic lesions) [12, 13]. Taken in the parasagittal plane these cores sampled a portion of the peripheral zone but also included a significant amount of tissue from the transitional zone, with subsequent studies of radical prostatectomy specimens demonstrating that the vast majority of adenocarcinomas arise in the posterolateral peripheral zone [14], thus explaining some of the false-negative results of standard sextant biopsy [15]. DOI 10.18502/sjms.v12i1.856 Page 27 Sudan Journal of Medical Sciences Production and Hosting by Knowledge E 2. Materials and Methods This is a prospective, descriptive, hospital based study-carried out in (GHRDS) in Wad Medani, Gezira state, Sudan. Gezira hospital for renal disease and surgery it serves patients from Gezira state which is the second popular state in Sudan with population of 4 million capita, also serves patients coming from nearby states . All patients, were above fifty, presenting with lower urinary tract symptoms sugges- tive of prostatic problems, were seen in the referred clinic. History, clinical examination including digital rectal examination (DRE) was done, investigations including, prostate specific antigen, and abdominal ultrasound were done. Patients indicated for TRUS/BX are selected, prepared through clinical work up, including history and examination to exclude contra-indications like bleeding tendency, painful anorectal conditions, and urinary sepsis. Written consent for the procedure and the study were obtained. Patients used to come early in the morning fasting, enema were routine, followed by an intra- venous injection of gentamycin. In left lateral position with flexed right hip and knee- rectal probe, covered with sterile condom was introduced lubricated with xylocaine jelly. Sextant biopsy was taken according to the Sudanese guidelines policy. More biopsies were taken in the presence of when palpable nodules, or suspicious hypo- echoic, hyper-echoic, calcifications or cyst formation. The procedure of TRUS/BX was done by the urologist. Biopsies were preserved in formalin (10%) containers sent for histopathology. Data were statistically analyzed using SPSS packages, the Chi square test was used for categorical variables .Variable was considered to be significant if the P value was 0.05 or less. The sensitivity and specificity and positive predictive value were calculated by using the formula: Sensitivity = TP/TP+FN Specificity = TN/FP+TN Positive predictive value = TP/TP+FP TP = True positive FN = False negative TN = True negative FP = False positive 3. Results The study included 297 patients who underwent transrectal ultrasound and biopsy at Gezira Hospital for Renal Diseases and Surgery, during the period from June 2006 to June 2009. The ages of the patients ranged between 50 – 70 years. DRE was found in 145 (48.8%) to be normal, 135 (93.1%) of them had histopathology results but 10 (6.9%) revealed cancer of the prostate . DOI 10.18502/sjms.v12i1.856 Page 28 Sudan Journal of Medical Sciences Production and Hosting by Knowledge E DRE Histopathology Total Benign Adenocarcinoma Inflammatory Normal 135 (93.1%)* 10 (6.9%) 0 145 Hard nodular 81 (87.1%)* 9 (9.7%) 3 93 Obliteration median sulcus 17 (53.1.1%) 15 (46.9%) 0 32 Fixed mucosa +pelvic extension 6 (23.1%) 21 (76.9%)* 0 27 239 55 3 297 T 1: The relation between the DRE findings and the histopathology of 297 patients had TRUS/BX. DRE sensitivity = 78.6%, DRE specificity = 56.3%, *P value=0 .000. PSA ng/ml Histopathology Total Benign Adenocarcinoma Inflammatory < 4 16 (84.2%)* 3 (15.8%) 0 19 4 to 10 115 (90.6%)* 12 (9.4%) 0 127 11 to 100 106 (70.7%)* 41 (27.3%) 3 150 > 100 1 (100%) 0 0 1 238 56 3 297 T 2: The histopathological distribution of 297 TRUS/BX biopsies according to the PSA level. PSA sensitivity = 94.5%, PSA specificity = 76.0%, *P value=0.001. Patients with abnormal DRE findings comprise 151 (50.8%), of them 107 (70.9%) had benign and 44 (29.1%) had malignant, as shown in Table (1), the sensitivity of DRE was 78.6 and the specificity was 56.3. PSA less than 4 ng/dl was found in 19 (6.4%) case, 3 (15.8%) had cancer prostate on histopathology, PSA level 4-10 ng/dl was found in 127 (42.8%) case, 12(9.4%) of them had cancer prostate. PSA level 11-100 ng/dl was found in 150 (50.5%) case, 41(27.3%) of them had cancer prostate, and 3 cases showed inflammatory changes. One case (0.3%) has reported more than 100 ng/dl of PSA, but his histopathology result was benign. The relation between stocktickerPSA and histopathology results was significant (P= 0.001) as shown in Table 2, the PSA sensitivity and specificity were 94.5% and 76.0 % respectively. Ultrasound was reported 210 cases as normal echo texture of the prostate,10 (4.8%) of them had cancer prostate. The abnormal ultrasound findings was in 87- patient Ultrasound Finding Histopathology Total Benign Adenocarcinoma Inflammatory Large normal texture 199 10 (4.8%) 1 210 Hypo-echoic lesion 37 17 (19.5%)* 2 56 Calcification + cyst 2 29 (33.3%)* 0 31 238 29+17=46 (52.8%)* 3 297 T 3: TRUS findings correlated to the histopathology of 297 TRUS/BX. TRUS sensitivity = 91.1%, TRUS specificity = 83.1%, P value = 0.000. DOI 10.18502/sjms.v12i1.856 Page 29 Sudan Journal of Medical Sciences Production and Hosting by Knowledge E 46(52.2%) of them were adenocarcinoma .Hypo-echoic lesions were found to be in 56- case, 17 of them had cancer prostate , and only two cases were inflammatory. Calcifications and cysts were seen in 31-case, 29 (33.3%) of them had cancer prostate. The relation between TRUS and histopathology results was significant (P value 0.000). The sensitivity and specificity of TRUS in this study were 91.1 and 83.1 respectively as shown in Table 3. 4. Discussion Introduction of PSA screening, and TRUS/BX in GHRDS changed the picture in detecting cancer prostate patients in an earlier stage, together with the ability to increase the number of patients who are candidates for prostatic biopsy i.e. to include patients with abnormal digital rectal examination findings, patients with stocktickerPSA level more than 4ng/ml, and patients with abnormal transrectal ultrasound echo texture. This practice consisted with the European guidelines of Urology. DRE is the most impor- tant part in assessing patients with prostate enlargement, and remained an essential predictor of cancer prostate. This is well documented in this study, which showed significant correlation between abnormal DRE findings and cancer prostate (CaP) (P- Value 0.000). PSA is an important predictor of prostate cancer, since the introduction of PSA as an screening tool, the incidence of cancer prostate jumped to be the leading cancer in males above 65 years old [1]. We consider the level 4ng/ml as a cut off value, but as shown in many studies [7] levels between (2 to 4 ng/ml) in younger ages i.e. less than 60 years old have significant number of cancer prostate. Therefore, we should reduce the cutoff value for younger patients, and at the same time take higher levels for old patients [4]. To increase the sensitivity of PSA as a predictor for cancer prostate, we can use more sensitive predictors like free PSA level/Total PSA ratio, PSA density, and PSA density of the transitional zone. This will reduce about 20% unnecessary biopsies [9, 10]. Transrectal ultrasound screening of the prostate improved the biopsy technique very much, where you can direct the needle targeting specific areas like the para sagittal zone to take the sextant biopsies , also you can direct the needle towards suspicious areas detected by the ultrasound screen i.e. hypo- echoic, hyper-echoic regions, and cysts or calcification zones. Transitional zone can be included with sextant biopsy, this explain the high negative biopsies in the classical sextant biopsies [15]. The combination of DRE, PSA, and TRUS as diagnostic procedures used to screen population for cancer prostate, showed that the positive predictive value increases as the number of abnormal modalities increases i.e. if one of the modalities is abnormal the positive biopsy rate is 6-25%, with two abnormalities it is 18-60%, and if all three modalities are positive, it is 56-72%. This shows that combination of the three diag- nostic modalities give the highest chance for detecting early cases of cancer prostate, and this what was done in our study [16, 17]. DOI 10.18502/sjms.v12i1.856 Page 30 Sudan Journal of Medical Sciences Production and Hosting by Knowledge E 5. Conclusions PSA level is highly sensitive but less specific in detection of prostate cancer. Normal DRE doesn’t exclude prostate cancer, fixed mucosa and obliterated median sulucus has the highest predictors of cancer prostate in DRE. Presence of calcifications and cyst on trans-rectal ultrasound has the highest liability for cancer prostate in compare to the other ultrasonic findings. 6. The Author Contributions i. Study concept and design: 1. Walaa Eldin Ibraheem 2. Prof. Mustafa Omran 3. Dr.Sami Mahjoub Taha ii. Collection of data: 1. Walaa Eldin Ibraheem 2. Dr.Sami Mahjoub Taha iii. Analysis and interpretation of data: 1. Walaa Eldin Ibraheem 2. Dr.Sami Mahjoub Taha References [1] D. M. Parkin, F. I. Bray, and S. S. Devesa, “Cancer burden in the year 2000 the global picture,” Eur J Cancer, vol. 37, 8, pp. 4–66, 2001. [2] D. Abuidris, E. Mohammed Imam, and O. E. Mustafa, “Elgaylani Ahmed Elhag, The Impact of TRUS in detection of prostate cancer in Gezira, Sudan,” GJHS, vol. 6, no. 1, pp. 35–42, 2010. [3] R. R. John, J. H. Elhan, and G. G. 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Brawer, “Diagnosis of prostatic carcinoma: The yield of serum prostate specific antigen, digital rectal examination and transrectal ultrasonography,” Journal of Urology, vol. 152, no. 5 I, pp. 1520–1525, 1994. [16] C. Mettlin, G. P. Murphy, R. J. Babaian et al., “The results of a five-year early prostate cancer detection intervention,” Cancer, vol. 77, no. 1, pp. 150–159, 1996. [17] W. T. Jones and M. I. Resnick, “Prostate Ultrasound in screening, diagnosis and staging of prostate cancer,” Probl Urol, vol. 4, pp. 343–357, 1990. DOI 10.18502/sjms.v12i1.856 Page 32 Introduction Materials and Methods Results Discussion Conclusions The Author Contributions References