Electromagnetic Modeling of the Propagation Characteristics of Satellite Communications Through Composite Precipitation Layers Science and Technology, 8 (2003) 1-10 © 2003 Sultan Qaboos University Effect of Cold and Hot Temperature on Behavioral and Selected Physiological Measures of Uromastyx aegyptius (Agamidae) A.A. El-Banna and A.M. Al- Johany Department of Zoology, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451, Saudi Arabia, Email: ajohany@ksu.edu.sa. أثر درجة الحرارة الباردة والعالية على السلوك وبعض القياسات الفسيولوجية للضب المصري أمين عبدالحميد البنا وعوض الجهني . تمـت دراسة االستجابات السلوكية والفسيولوجية والكيمياحيوية لدرجات الحرارة الباردة والعالية للضب المصري :خالصـة Uromastyx aegyptius . ـ باإلضافة إلى مجموعة ثالثة ضابطة . م تقسيم اثني عشر ضباَ إلى مجموعتين اختباريتينوقـم ت جمعت . م ° 22 ساعة للتأقلم عند درجة حرارة 24وقد أعطيت الثالث مجموعات فترة . م ° 22حفظـت تحت درجة حرارة في ) P<0.05(ت النتائج فروقاً معنوية عينات الدم من كل حيوان عندما تصل درجة حرارة الجسم الدرجة المحددة ، وقد أظهر هذا االنخفاض في مواد التمثيل خارج ) م2º(انخفاض جلوكوز الدم ومستوى الكولسترول خالل فترة تعرض الحيوانات للبرودة أدى إلى زيادة معنوية ) م45º(أن رفع درجة حرارة الحيوان إلى . الخاليا يعكس زيادة في امتصاص الخاليا لهذه المواد )P<0.05 ( هذه التغيرات يمكن أن تعزي إلى زيادة فقد الماء بالتبخير . فـي كمـية البروتين الكلي واليوريا وحمض اليوريك خالل T3 ،T4في ) P<0.05(لم تظهر أي تغيرات معنوية . وبخاصة بسبب زيادة اللهث وما يتبعه من نقص في حجم البالزما ج هذه الدراسة إلى زيادة في حجم األيض الالهوائي في الضب خالل فترة التبريد بديل وقد أشارت نتائ . فترة التبريد أو التسخين نقص مستوى الجلوكوز من خارج الخاليا إلى داخل الخاليا مما يشير إلى استعداد الحيوان لتفادي حدوث التجمد الكامن لكي تتم . حماية الحيوان من تجمد السوائل داخل الخاليا ABSTRACT: The behavioral, physiological and biochemical response to low (2°C) and high (45°C) temperatures was studied in Uromastyx aegyptius. Twelve animals were divided into two experimental groups. A third control group was kept at 22 °C. All animals in the cooling, warming, and control groups were allowed a period of 24 hours for adjustments at 22 °C. Blood samples were collected from each animal when body temperature reached the corresponding levels. The results showed a significant (P<0.05) decrease in blood glucose and cholesterol levels during cooling (2°C). This reduction in extracellular fluid substrates reflects an increase in cellular uptake of these substrates. Warming (45°C) resulted in a significant (P< 0.05) increase in total proteins, urea, and uric acid. These later changes could be attributed to an increase in the evaporative water loss, particularly due to the increased observed panting, and the associated reduction in plasma volume. There were no significant (P>0.05) changes in T3 and T4, during cooling nor during warming. The results of this study suggest augmentation of anaerobic metabolism of the U.aegyptius during cooling as evident by reduction in blood glucose levels. Furthermore, shift of glucose from the extracellular to the intracellular fluids demonstrates anticipation against potential freezing in order to protect the animal from intracellular freezing. KEYWORDS: Metabolism, Temperature; Behaviour; Blood Constituents; Plasma; Uromastyx; Lizard. 1. Introduction The spiny tailed lizard Uromastyx aegyptius is the largest agamid lizard and is widespread in Arabia (Arnold, 1986). It is a diurnal animal and becomes active during the warm part of the day. Many terrestrial ectotherms, like the U. aegyptius, have a considerable capacity for behavioral and physiological thermoregulation (Bartholmew, 1981). Although many ectotherms are 1 A.A. EL-BANNA and A.M. AL-JOHANY physiologically adapted to survive at low ambient temperatures, terrestrial ectotherms are particularly susceptible to freezing, and there are a variety of strategies that enable ectothemic animals to survive freezing conditions (Hew et al. 1986). Ectotherms exposed to high temperature can either attempt to regulate their body temperature below the air temperature or rely on biochemical adaptation for tolerance of high body temperature (Cossins and Bowler, 1987). Additionally, high temperature causes proteins to unfold and hence enzymes protein can no longer function. However, heat shock protein (HSP) response by programming cellular activities that bind and help mutated protein to restore their shape. Ulmasov, et al., (1992) and Zatespina, et al., (2000) found that HSP plays a major role in the cellular response during heat stress. Their findings also included a positive relationship between HSP and the magnitude of thermotolerance among lizard species. We thought that HSP dynamic relates to total plasma protein, and related measures such as albumin, and globulin. Thyroid hormones are involved indirectly in thermoregulation by increasing metabolic rate and subsequently heat production. Despite this, controversy exist regarding the initial calorigenic effects of thyroxin. Sinha and Choubey (1981) reported a positive relationship between thyroid activity and environmental temperature in U. hardwickii lizards. As thyroxin enhances cellular absorption of glucose and also influence plasma cholesterol, we included these measures in our study. Thermoregulatory adjustment of the lizards are behavioral or physiological changes to deflect the body temperature from environmental heat loads (Firth and Belan, 1998). Measurements of blood composition are commonly used to assess the way in which the environment affects the physiology of animals. Therefore the objective of the present study was to investigate some aspects of physiological and biochemical responses of the U.aegyptius when exposed to low and to high temperatures. 2. Materials and methods Eighteen U. aegyptius (mean wt. 760 gms) were collected from the field (Rumah and Haradh townships around Riyadh city, Saudi Arabia). The animals were housed in an open vivarium (2m x 1/4 m) with overhead heat and light lamps with timer control set at 12:12 for light and dark periods and kept at a constant air temperature of 22°C (room temperature). The animals were then divided into three groups (6 animals in each) of matched body weight. Blood samples were collected from animals in the control group at 22°C. Animals in the two experimental groups were exposed to the low and high temperatures, as described below. Body temperature was maintained at 45°C for the warm group and 2°C for the cold group for 24 hrs and 48 hrs, respectively. Blood samples were collected at the end of exposure time. All blood samples were collected by cardiac puncture method using vacutainers. The vials were kept at room temperature for one to two hours, then, centrifuged at 3000 rpm for 10 minutes to separate the plasma which was stored at - 20°C for later analysis. 2.1. Cooling procedure: exposure to low temperature All animals of the second group were placed in Plexiglas chamber. A YSI model 423 thermocouple temperature probe was inserted 2 cm into the cloaca of the animal and held tight with a tape on the tail of the animal. The probe was attached to an YSI telethermometer (Model 44) in conjunction with a Omni scribe chart recorder to monitor the internal body temperature of the animal. Each animal was placed on a multiple benched mini-cold lab model 2203 (LKB-Produkter AB), and the temperature was set to 2ºC. Animals were left in this environment for 48 hours before taking the blood sample. 2.2. Warming procedure: exposure to the high temperature The same probe and recording instruments were tied to the animals while exposing them to the high temperature. A Plexiglas chamber animal was fixed in a water-bath (Gallenkemp Gmbh) with temperature control thermostat set at 45ºC. Each animal was introduced into the chamber and then 2 EFFECT OF COLD AND HOT TEMPERATURES the temperature was gradually raised till the water-bath temperature and the animal body temperature reached 45 ±1ºC. After constant exposure to this temperature for 24 hr, blood samples were collected. 2.3. Analysis of plasma The following components were determined quantitatively in the plasma collected from the three groups of U.aegyptius, using the BioMerieax Vitek (Missouri, USA) kits. Total protein following the principal of Biuret reaction according to Peters (1968); albumin according to Drupt et al. (1974); triglyceride according to Fossati and Prencipe (1982); cholesterol according to Anonymous (1989); glucose according to Tender (1969); urea according to the modified Berthelot reaction after Patton and Crouch (1977); uric acid as described by Artiss and Entwistle (1981). T3 and T4 were determined using enzyme immuno assay (EIA). Plasma globulin concentration was calculated by subtraction. Fractionation of total protein was carried-out using cellulose acetate electrophoresis (Helena Lab. Instruments Ltd. U.K), and the electropherograms were then scanned using Personal densitometer SI (Amersham Pharmacia Biotech), and the percentages of the different protein fractions were determined. 2.4. Statistical analysis Analysis of Variance (ANOVA) was utilized for testing the overall effects of cooling and warming on selected blood measures. Post-hoc comparisons were conducted between pairs of means, via LSD procedure, on total protein, albumin, cholesterol, globulin, urea, uric acid, T3, and T4. 3. Results 3.1 Behaviour U.aegyptius are known to tolerate extreme low and high temperatures (Bartholmew, 1981). The results of this study showed that when the animals were exposed to low temperature (2°C), they became lethargic and immobile. No change of color was noticed during this period. At 2°C blood circulation was very slow and the animal needed to be rubbed on the neck and back to facilitate the flow, when drawing blood. At high temperature U.aegyptius were noticed to fight for release when body temperature reached 40°C. Defecation was observed after two hours of high temperature exposure. They started to pant when temperature crossed the 40°C limit. Body colouration changed from a dark tan to pale yellow at 42°C. 3.2. Blood components Table 1 shows the concentrations of plasma proteins in U.aegyptius exposed to the low and high temperatures compared to that in U.aegyptius exposed to the room temperature (22°C). No significant difference was observed in plasma total proteins between animals exposed to the 2°C and the 22°C, but there was a significant (P<0.05) increase in plasma total proteins when animals were exposed to the 45°C. The main increase in plasma total proteins at the 45°C was due to an increase in albumin and α 1-globulin fractions. Plasma albumin and the albumin/globulin ratio were significantly (P<0.05) lower in animals exposed to the 2°C (Table 2). The electrophoretic separation of plasma proteins indicates that there was a significant decrease (P<0.05) in the α 2-globulin fraction when animals were exposed to either the 2°C or the 45°C. No significant change was observed in the percentages of the β and γ-globulin fractions when animals were exposed to the three different temperatures (Table 2). The mean concentrations of some plasma constituents in U.aegyptius exposed to the three different temperatures are presented in Table 1. There was no significant (P>0.05) difference in the concentrations of plasma triglycerides in U.aegyptius exposed to the different temperatures (Table2). Plasma cholesterol concentrations in animals exposed to the 22°C and the 45°C were 3 A.A. EL-BANNA and A.M. AL-JOHANY about the same, but a significant (P<0.05) decrease in cholesterol concentration was found in animals exposed to the 2°C (Table 3). The mean plasma glucose concentration in U.aegyptius at room temperature (22°C) was significantly (P< 0.05) decreased in animals exposed to the 2°C, and was significantly (P<0.05) increased in animals exposed to the 45°C (Table 3). Table 1: Mean concentration of plasma proteins in U.aegyptiuss exposed to low, Room and high temperature. Temperature Measure Cold (2°C) Room (22°C) Warm (45°C) Total Protein (g/dl) 2.53 ± 0.27 2.90 ± 0.15 3.65 ± 0.17 Albumin (g/dl) 0.98 ± 0.09 1.27 ± 0.10 1.67 ± 0.09 Globulin (g/dl) 1.98 ± 0.30 1.74 ± 0.19 2.30 ± 1.10 Albumin/Globulin ratio 0.49 ± 0.06 0.72 ± 0.05 0.73 ± 0.05 Triglyceride (mg/dl) 19.2 ± 3.5 18.3 ± 4.0 22.5 ± 1.8 Cholesterol (mg/dl) 104.7 ± 15.9 234.3 ± 28.3 226.4 ± 28.4 Glucose (mg/dl) 206 ± 8.8 281.9 ± 16.8 358.0 ± 12.8 Urea (mg/dl) 12.3 ± 1.3 8.8 ± 0.5 12.0 ± 8.0 Uric Acid (mg/dl) 2.8 ± 0.3 3.1 ± 0.4 5.8 ± 0.7 T3 (ng/dl) 7.0 ± 0.5 7.6 ± 1.0 7.6 ± 0.8 T4 (µg/dl) 3.0 ± 0.2 4.1 ± 0.4 5.4 ± 0.3 Table 2: Summary table of ANOVA conducted on Dependent Variables (measures). Measure Source of Variation SS df MS F(value) F(probability) Total Protein Between Group Within Group 4.32 3.85 2 16 2.15 0.24 17.21* 3.63 Albumin Between Group Within Group 1.42 0.87 2 16 0.71 0.05 12.29* 3.63 Globulin Between Group Within Group 1.03 5.32 2 16 0.51 0.33 1.54 3.63 Albumin / Globulin Ratio Between Group Within Group 0.36 1.28 2 16 0.18 0.08 2.25 3.63 Glucose Between Group Within Group 74174.67 17467.53 2 16 37087.34 1091.72 33.97* 3.63 Triglycerides Between Group Within Group 51.69 989.49 2 16 25.84 61.84 .41 3.63 Cholesterol Between Group Within Group 64793.73 65184.06 2 16 32396.86 4074.00 7.97* 3.63 Urea Between Group Within Group 45.62 82.16 2 16 22.81 5.14 4.44* 3.63 Uric Acid Between Group Within Group 35.68 28.36 2 16 17.84 1.77 10.06* 3.63 T3 Between Group Within Group 1.25 60.09 2 16 0.62 3.75 0.16 3.63 T4 Between Group Within Group 18.84 78.26 2 16 9.42 4.89 1.92 3.63 * P<0.05 4 EFFECT OF COLD AND HOT TEMPERATURES There was a significant (P<0.05) increase in the mean plasma urea concentration when U.aegyptius were exposed to either the 2°C or to the 45°C (Table 3). Also, there was a significant (P<0.05) increase in the mean plasma uric acid concentration when U.aegyptius were exposed to the 45°C, but no change in plasma uric acid was observed in animals exposed to the 2°C and 22°C (Table 3). Table 3: Multiple Comparisons of Differential Mean Effect Using LSD Method for only Those Variable which Were Significantly Different. Total Protein Albumin Glucose Group Mean Diff. Sig. Group Mean Diff. Sig. Group Mean Diff. Sig. LT RT HT -0.5333* -1.2905* 0.034 0.000 LT RT HT -0.4333* -0.7988* 0.033 0.000 LT RT HT -74.700* -151.328* 0.001 0.000 RT LT HT 0.5333* -0.7571* 0.034 0.004 RT LT HT -0.4333* -0.3655 0.033 0.058 RT LT HT 74.700* -76.628* 0.001 0.001 HT LT RT 1.2905* 0.7571* 0.000 0.004 HT LT RT 0.7988* 0.3655 0.000 0.058 HT LT RT 151.328* 76.628* 0.000 0.001 Cholesterol Uric Acid Group Mean Diff. Sig. Group Mean Diff. Sig. LT RT HT -129.746* -121.728* 0.003 0.003 LT RT HT -0.2667 -2.9657* 0.733 0.001 RT LT HT 129.746* 8.018 0.003 0.824 RT LT HT 0.2667 -2.6990* 0.733 0.002 HT LT RT 121.728* -8.018 0.003 0.824 HT LT RT 2.9657* 2.6990 0.001 0.002 *The mean difference is significant at the 0.05 level. The mean plasma T3 and T4 concentrations were not significantly different among animals exposed to the three different temperatures, although T4 concentrations were somewhat higher at the 22°C and the 45°C (Table 2). 4. Discussion In their natural habitat, U.aegyptius are exposed to low or high temperatures. In many instances they continue activity while air temperature is around 40°C and above (Louw and Gideon, 1993). They remain in their burrows when surface temperature falls below 10°C. They are not true hibernators; they may come out during winter when it is warm enough like many other lizards. They usually become active when temperature reaches 30°C, and when temperatures reache above 40°C they seek shelter in their deep underground burrows. In our study, a significant decrease in blood glucose and cholesterol levels were found in U.aegyptius exposed to 2°C for about two days. This was also associated with a significant decrease in albumin fraction of the plasma proteins, and with a significant increase in the levels of plasma urea. On the other hand, a significant increase in blood glucose, as well as a significant increase in plasma total proteins, urea and uric acid were found in U.aegyptius exposed to 45°C for 24 hours. To adjust for changes in environmental temperature many ectothermic vertebrates 5 A.A. EL-BANNA and A.M. AL-JOHANY lower the selected body temperature during winter (Hazel and Prosser, 1974; Case, 1976). Ectotherms exposed to higher temperatures attempt to regulate their body temperature below the air temperature (Huey, 1982). These alterations in the selected body temperature lead to blood biochemical changes. Such modification seem to be achieved by metabolic changes which lead to changes in the composition of blood and tissues (White and Somero, 1982; Geiser et al., 1992). Therefore, measurements of blood composition are commonly used to assess the way in which the environment affects the physiology of animals. Total Protein (TP) 0 0.5 1 1.5 2 2.5 3 3.5 4 2 C Degrees 22 C Degrees 45 C Degree Albumin (Alb) 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 2 C Degrees 22 C Degrees 45 C Degree Plasma Glucose (Glu) 0 100 200 300 400 500 2 C Degrees 22 C Degrees 45 C Degree Uric Acid (UA) 0 1 2 3 4 5 6 7 2 C Degrees 22 C Degrees 45 C Degree C o l e s t e r o l ( C h o l ) 0 5 0 1 0 0 1 5 0 2 0 0 2 5 0 2 C D e g r e e s 2 2 C D e g r e e s 4 5 C D e g r e e T P (m g\ dl ) A lb (g m \d l) U A (g m \d l) G lu (g m \d l) C ho l (m g\ dl ) Figure 1. Plot of the changes in total protein (TP), Albumin (Alb), Glucose (Glu), uric acid (UA), and cholesterol (Chol) among variation in environmental temperature. When lizard species normaly encounter temperatures close to or below 0°C, they have exceptionally high survival rates (Geiser and Firth, 1992). These lizards survive exposure to subfreezing temperatures by supercooling, i.e., by remaining unfrozen at temperatures below the equilibrium crystallization temperature of body fluids (Grenot and Heulin, 1990). Also, some lizards are freeze-tolerant (Storey and Storey, 1992; Costanzo et al., 1995). Ectotherms can counteract a potentially freezing ambient temperature by increasing the osmotic concentration o 6 f EFFECT OF COLD AND HOT TEMPERATURES their body fluids so that its freezing point is depressed below the ambient temperature (Withers, 1992). Some ectotherms accumulate high concentration of specific solutes to depress their freezing point; these solutes are typically sugar, such as glucose or sugar alcohols. Their low molecular weight maximizes the freezing point depression per mass of solute (Withers, 1992; Lee and Costanzo, 1993). These sugars apparently have cryoprotectant effect, and they protect membranes and enzymes against cold denaturation and cold shock injury. It is important in these animals to avoid the freezing of their intracellular fluids, since intracellular ice crystals apparently destroy the integrity of intracullar membranes and organelles. In contrast, extracellular freezing does not disrupt essential cell structures and is not lethal, at least to the freeze-tolerant species (Withers, 1992; Lemos-Espinal and Ballinger, 1992). The decrease in blood could reflect a shift of glucose from the blood (extracellular fluid) to the intracellular fluids, probably through increasing cellular uptake of glucose, in anticipation of potential freezing ambient temperature. In doing that, the animals guard against intracellular freezing by depressing intracellular freezing point. The accumulation of the putative cryoprotectant glucose in tissues of lizards subjected to freeze tolerance trials had been reported (Costanzo et al. 1995). The mean blood cholesterol level in U.aegyptius at room temperature (234.3 ± 28.3 mg/dl) is relatively higher than that in many mammalian species (Al-Tuwaijri, 1987). Also, cholesterol content in U.aegyptius’s tissues is more than twice the cholesterol content of camel, lamb and cattle tissues (Abu-Tarboush et al. 1996). Cholesterol could also have cryoprotectant effect in lizards exposed to low temperatures, and the changes in blood cholesterol level observed in the present study may indicate a role for cholesterol in thermoregulation. Changes in the lipid composition of tissues and cell membranes are apparently required in order to maintain normal function at the lower body temperatures (Bauwens, 1981). Lizards exposed to high temperature try to reduce their body temperature below the air temperature, and this strategy of thermoregulation can only be accomplished by evaporative cooling. Evaporation of water can dissipate a considerable amount of heat and lower the temperature (Withers, 1992). Relatively waterproof terrestrial animals are generally able to enhance evaporative cooling by markedly increasing their cutaneous or respiratory water loss. The lizard Dipsosaurus totally dissipates its metabolic heat production by panting at air temperature more than 40°C, and the large agamid lizard Amphibolurus can reduce its body temperature about 3.2°C below air temperature of 43°C, and the chuckwalla lizard Sauromalus obseus can considerably lower its body and brain temperatures by evaporative cooling by panting (Withers, 1992). In this study evoparative water loss (EWL) was not measured. However, we observed, particularly because of the increased panting, that the reduction in plasma volume could be the reason for the increased concentrations of plasma glucose, total proteins, urea and uric acid observed in U.aegyptius exposed to the 45°C. During dehydration, animals show elevated concentrations of osmolytes and proteins in their blood (Dunlap, 1995). The increased plasma glucose concentration in U.aegyptius exposed to the higher temperature could also reflect a decrease in glucose uptake by tissues in order to avoid excess intracellular glucose breakdown and energy production, and excess heat load. Lizards exposed to high temperature can also rely on biochemical adaptations for tolerance of high body temperature. Heat stress has been shown to induce the synthesis of a family of proteins, the so-called heat shock proteins (HSPs) in cells of a wide spectrum of ecologically different lizard species (Ulmasov et al. 1992; Feder and Hofmann, 1998; Zatsepina et al. 2000). The level of HSPs were found to be higher in desert species than in the non-desert species (Zatsepina et al. 2000), and the induction and active synthesis of HSPs occurs within a temperature interval normal to the species (Ulmasov et al. 1992). HSPs play a key role in the cellular response to heat shock by maintaining the native state and proper folding of cellular proteins during physiological stress and by facilitating the restoration of cellular functions (Morimoto, 1993; Morimoto, et al., 1994). The expression of the HSP gene is tightly regulated to ensure that the response is proportional to the level of heat stress, and then repressed and terminated when normal physiological conditions recur (Lindquist, 1986). We did not measure the synthesis of HSPs in the present study, but these 7 A.A. EL-BANNA and A.M. AL-JOHANY proteins can be synthesized, particularly by liver cells, within 1 to 2 hrs of exposure to the high temperature, and in desert lizards, the temperature range of induction and continuing synthesis of HSPs was reported to be 36-50°C (Ulmasov, et al., 1992). The changes in plasma protein and protein fractions observed in U.aegyptius exposed to the 45°C in the present study could reflect induction and synthesis of HSPs. U.aegyptius exposed to different temperatures in the present study showed no changes in plasma levels of the thyroid hormones T3 and T4. It is not certain whether the thyroid hormones have a direct and immediate role in thermoregulation in lizards. Sinha and Choubey (1981) reported that the thyroid gland of the Indian spiny tailed, U. harrdwickii, lizard appeared most active when the environmental temperature was high. When the temperature was low, the activity of thyroid gland was decreased. These authors also noted a reduction in thyroid activity in September. They did not reach a final conclusion because they found a parallel relation to the gonadal cycle. 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ZATESPINA, O.G., ULMASOV, K.A., BERESTEN, S.F., MOLODTSOV, V.B., RYBTSOV, S.A. and EVEGENEV, M.B. 2000. Thermotolerant desert lizards characteristically differ in terms of heat-shock system regulation. Journal of Experimental Biology, 203: 1017-1025. Received 18 May 2002 Accepted 19 April 2003 10 Effect of Cold and Hot Temperature on Behavioral and Selected Physiological Measures of Uromastyx aegyptius (Agamidae) A.A. El-Banna and A.M. Al- Johany Department of Zoology, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451, Saudi Arabia, Email: ajohany@ksu.edu.sa. 2. Materials and methods 2.1. Cooling procedure: exposure to low temperature 2.3. Analysis of plasma Analysis of Variance (ANOVA) was utilized for testing the overall effects of cooling and warming on selected blood measures. Post-hoc comparisons were conducted between pairs of means, via LSD procedure, on total protein, albumin, cholesterol, globulin 3. Results 3.1 Behaviour U.aegyptius are known to tolerate extreme low and high temperatures (Bartholmew, 1981). The results of this study showed that when the animals were exposed to low temperature (2(C), they became lethargic and immobile. No change of color was noticed 3.2. Blood components Temperature Between Group Between Group Between Group Between Group Between Group Between Group Between Group Between Group Between Group Between Group Within Group Between Group Within Group 4. Discussion References