Photovoltaic Cells and Systems: 13-23 SQU Journal for Science, 16 (2011) © 2011 Sultan Qaboos University 13 Micellar Enhanced Ultrafiltration for the Removal of Polycyclic Aromatic Hydrocarbons (PAHs) Mixtures in Underground Contaminated Water in Oman Mohamed Aoudia*, Amal Al-Sabahi**, Salma Al-Kindy, Mahfoodh Al-Sheily and Fouzul Marikar Department of Chemistry, College of Science, Sultan Qaboos University, P.O. Box 36, Postal code 123, Muscat, Sultanate of Oman,*Email: aoudia@squ.edu.om.**Ministry of Education, Muscat, Sultanate of Oman. حلقات فً المٌاه الجوفٌة الملوثةمتعددة ال معالجة الهٌدروكربونات األروماتٌةاستخدام طرٌقة الترشٌح الدقٌق المعزز بالمسٌل ل فً عمان فازول مارٌكارو أمل الصبحً، سلمى الكندي، محفوظ الشعٌلً ،محمد أودٌع فً منطقة الحلقات ةالمتعددتٌة اوكربونات األرومفً محاولة لتحلٌل المٌاه الجوفٌة الملوثة بكمٌات من الهٌدر :ملخص تٌة االهٌدروكربونات األروماستخدام الكروماتوجرافٌا الغازٌة المتزامنة مع طٌف الكتلة لتحدٌد تركٌز عناصر تم ،الرستاق ٌد زمن تحصلنا على تحد .الحلقات هٌدروكربونات متعددة 61الموجودة فً خلٌط معٌاري ٌحتوي على الحلقات ةالمتعدد ٌِّنات من تٌة اككل الهٌدروكربونات األروم رٌالمعاٌ ىومنحن االحتجاز المٌاه ثم اسُتـخدمت لمعرفة المركبات الموجودة فً ع الهٌدروكربونات " المستحدثة لمعالجةالّدقٌق المعّزز بوجود "المٌسل ها و قد استعملت طرٌقة الّترشٌحالجوفٌة الملوثة و تركٌز تٌة المتعددة امن الهٌدروكربونات األروم )فً البلٌونواحد جزء ( مائٌة ذات تركٌز منخفضال الحلقات ةددالمتعتٌة ااألروم ٌَة فقد لوحظ أنه تم .(Tween 80)المنشط السطحً باستخدامالحلقات فً حدود الكشف األدنى .احتجاز العناصر بصفة كل قل أالمتبقٌة الحلقات ةالمتعددتٌة االهٌدروكربونات األروم ر( كان التركٌز لعناصجزء فً البلٌون 0.01 ±المستعمل ) عند تطبٌق هذه ، بطرٌقة مماثلة .موح بها فً المٌاه الصالحة للشربق التوصٌات المسبهذا ٌطاو جزء فً البلٌون 0.01من الحلقات ةالمتعددتٌة اونات األرومالهٌدروكربوجدنا أّن تركٌز عناصر ، تاق( الرس)التقنٌة لعٌنة من مٌاه جوفٌة ملوثة بالدٌزل .(البنزو)أ( بٌرانول بالنسبة للعنصر األكثر سمٌة )و هو الحد األدنى المقب جزء فً البلٌون0.01 قل منأإلى انخفض ABSTRACT: In an attempt to analyze polycyclic aromatic hydrocarbons (PAHs) in diesel contaminated underground water in Oman (Rustaq), Gas chromatography-Mass spectrometry was first used to determine the different concentrations in a standard mixture containing 16 PAHs. Retention time and calibration curves were obtained for all aromatic compounds and were used to identify a given analyte as well as its concentration in the contaminated underground water. Micellar enhanced ultrafiltration (MEUF) was then used to treat standard aqueous solution MOHAMED AOUDIA et al. 41 of PAHs at low concentration (~ 1 ppb) using an edible nonionic surfactant (Tween 80). The totality of the mixture components was completely rejected. Within the experimental detection limit ( 0.01 ppb), the residual PAH concentrations were less than 0.01 ppb in accord with the allowed concentrations in drinking water. Likewise, excellent rejections of PAHs in MEUF treatment of diesel contaminated underground water at an Omani site (Rustaq) were observed. The concentration of PAHs was reduced to less than 0.01 ppb, the accepted limit for the most toxic member of the PAH group (benzo(a)pyrene). KEYWORDS: Surfactant; Micelle; Enhanced ultrafiltration; Rejection; Retention time. 1. Introduction ontamination of aquifers by toxic and/or hazardous organic pollutants such as gasoline and diesel fuels is becoming a critical environmental issue in Oman where about 14 contaminated sites have been identified by the Ministry of Regional Municipalities and Environment. Diesel-range petroleum fractions are extremely complex mixtures containing a large number of organic compounds. Polynuclear aromatic hydrocarbons (PAHs) which may represent about 60% of volume in diesel are of particular concern as they are either known or suspected carcinogens (Prak and Pritchard, 2002). Their removal presents a challenge to scientists and engineers owing to their low solubility in water and their great tendency to adsorb onto soil and sediments. Therefore, conventional techniques such as pump and treat have proven to be of limited practical value, and significant efforts are being devoted to the development of efficacious approaches for the remediation of PAH contaminated sites. Surfactant flushing was recognized as one of the most promising in situ remediation techniques for contaminated underground water, generating a great deal of interest in surfactant-enhanced aquifer remediation (SEAR) processes (Doung et al. 1996; Volkering et al. 1995; Zhao et al. 2005). Overall, SEAR strategies are commonly characterized by two sequential process stages in situ, namely desorption of the contaminant from the sediment surface and subsequent incorporation of the pollutant into the bulk aqueous phase. Ex-situ treatment can then be achieved by a surfactant-based process known as micellar enhanced ultrafiltration (MEUF). In this process (Baek and Yang, 2004; Aoudia et al. 2003), a surfactant at concentration higher than its critical micelle concentration (CMC) is added to the aqueous stream containing the dissolved hydrocarbons. The micelle causes the organic compounds to solubulize in the micellar core. The stream is then passed through an ultrafiltration membrane having pore sizes small enough to obstruct the passage of micelles, whereby the hydrocarbon molecules will be rejected. As a result, the permeate contains very low concentration of surfactant and organic solutes. Compared to other conventional separation techniques, MEUF is a relatively low energy, pressure driven, membrane-based separation process. Anisotropic membranes ranging in molecular weight cut-off (MWCO) from 5000 to 50,000 Daltons are generally used to reject micelles from the surfactant stream. However, one major limitation of MEUF is the inevitable leakage of monomer surfactant molecules into the effluent. Since the monomers (surfactant molecules dispersed in the bulk phase in equilibrium with micelles) are not significantly rejected by the membrane, the total surfactant concentration in the permeate is equal or slightly less than the surfactant critical micelle concentration (CMC). Indeed, this was clearly established by Fillipi et al. (1999). One option to tackle this disadvantage is to use biodegradable, non toxic surfactants having extremely low critical micelle concentration. The other option is to use membranes having small MWCO (e.g., 500-1000 Daltons) to recover the monomers for reuse. The efficiency of MEUF in water treatment is mainly determined by the extent of solubilization enhancement of organic pollutants in the presence of surfactant. Thus, numerous studies have investigated water solubility enhancement of single PAHs in the presence of surfactants above their critical micelle concentrations (Zhu and Feng, 2003; Aoudia and Al-Shaaili, 2006; Hill and Ghoshap, 2002; Aoudia et al. 2010). This solubility enhancement has been convincingly related to the surfactant and solute structure and to the PAH-micelle interaction (Prak and Pritchard, 2002). However, only a limited number of investigations have been reported C MICELLAR ENHANCED ULTRAFILTRATION 41 where effects of multiple solutes on micellar solubilization of an individual component were examined. Thus, solubilization of binary mixtures of hydrocarbon (benzene and hexane) in anionic and nonionic surfactants systems was investigated by Chaiko et al. (1984). The authors reported selective solubilization in some mixtures and a synergistic effect on the solubilization of hexane in the presence of small amounts of benzene. These studies showed evidence that the less hydrophobic solute (benzene) partitioned at the micellar core-water interface caused the interfacial tension to decrease, which consequently enabled the micellar core volume to increase, resulting in a greater solubility of the more hydrophobic compound (hexane). Solubilization of pyrene, fluoranthrene, and phenantrene from binary and ternary mixtures in nonionic surfactant solutions were investigated by Prak and Pritchard (2002). The extent of solubilization was shown to be greatly influenced by the PAH structure, PAH-PAH interactions, PAH-micelle interaction, and PAH packing within the micelle. Using such simple binary and ternary PAH mixtures, it was clearly demonstrated that the single- component micellar partition coefficient cannot be used to predict the multicomponent micellar solubilization. In the presence of a co-solute, the concentration of organic compounds in micellar solutions may decrease, remain the same, or increase over single component systems depending on the co-solute and the surfactant structures (Guha et al. 1998; Jacobson and Casassa, 1991; Underwood et al. 1993). Likewise, most MEUF studies focused on the removal of single PAH solutes from an aqueous stream (Dunn et al. 1985; Dunn et al. 1987; Khandori and Schechter, 1990; Edwards et al. 1991; Hong and Yang, 1994; Kim et al. 1998; Jachowska et al. 2002; Syamal and Bhattacharya, 1997). Very few addressed the rejection of PAHs in mixtures (Talens-Alesson et al. 2001; Bielska and Szymanowski, 2004; Jadhav et al. 2001) and evidence was found that removal of individual PAHs and other organic compounds when solubilized in mixtures is not related in a simple manner to their single solute rejection from aqueous systems. For instance, the removal of phenol, p-cresol, xylenol in simultaneous micellar-enhanced ultrafiltration process showed that the highest rejection was observed with the most hydrophobic xylenol (Witek et al. 2006). MEUF of different phenolic derivatives including meta- nitrophenol (MNP), catechol (CC), paranitrophenol (PNP), and beta-napthol (BN) in their binary mixture has been studied by Purkait et al. (2005). Retention of solutes was found to be less in the case of the two-component feed solution compared to the single-component feed solution, suggesting that even for simple multiple systems (binary and ternary), the rejection of each cannot be predicted by its rejection from single-component system. Clearly, binary and ternary PAH mixtures are not realistic models for their solubilization in diesel contaminated sites where PAHs mostly exist in mixtures containing a large number of organic compounds. Therefore, the main objective of this work is to investigate the efficiency of MEUF in removing PAH pollutants from a standard aqueous PAH mixture containing sixteen components using an edible surfactant (Tween 80). This mixture can be considered to be a realistic model mixture of PAH in the diesel-range petroleum fraction, a source of environmental concern in Oman due to leakage of underground gasoline station storage tanks in many sites in the Sultanate. The concentration of each PAH component will be purposely limited to about 1 ppb in order to assess the efficiency of the MEUF process in removing such extremely low amounts of organic solutes due to the fact that the recommended total PAHs concentration in drinking water supply is ~ 0. 20 ppb whereas that of their most toxic member benzo(a)pyrene is 0.010 ppb (Omani Standards for Unbotteled Drinking Water No. 245/1993). Furthermore, the removal of PAHs from diesel contaminated underground water in Oman (Rustaq) via micellar enhanced ultrafiltration will also be evaluated 2. Experiment 2.1 Materials Surfactant Tween 80 (Molecular weight of 1310 g/mol, CMC = 0.0013 g/dliter) was purchased from Sigma (USA) and used without further modification. Standard mixtures of polycyclic aromatic hydrocarbons were purchased from ULTRA Scientific (USA). The concentration of each mixture was 2000 μg/ml in methanol for each single compound. Dichloromethane (CH2Cl2, 99.5% pure) was supplied by S.d fine-chem Ltd (USA). Sodium hydroxide pellets (98.0 %) and sodium hypochlorite solution (12% chlorine) were obtained from Qualigens and BDH, respectively. Sodium sulphate anhydrous, 99 % (purchased from Qualigens) was used as MOHAMED AOUDIA et al. 41 received. Ultrapure water 18.2 M (millliQ Millipore corporation) was used to prepare standard solutions. The ultrafiltration membrane, supplied by Millipore, was regenerated cellulose with MWCO 10,000 Daltons (Type YM). The effective membrane area was 31.65 cm 2 . 2.2 Methods Ultrafiltration Ultrafiltration experiments were performed at room temperature using an Amicon 8200 batch stirred cell. The feed volume of the solution was 180 ml and the pressure in the batch cell was maintained at 80 –100 kPa by nitrogen gas. The ultrafiltration process began by placing the membrane with the glossy side facing upwards on the support at the bottom of the cell. The cell was then fixed tightly, filled with distilled water and pressurized with nitrogen gas. The water flux was measured to determine the permeability of the freshly used membrane. The feed solution was prepared by dissolving the required amount of surfactant in deionized water. The feed solution in the cell was stirred with a triangular stirrer at a constant stirring speed. The run was continued until 150 ml of the permeate (80% of the feed volume) had been collected, and the flux was monitored at different times during the entire ultrafiltration experimental run. After each run, the entire cell, stirrer and membrane were washed with distilled water to remove any deposition. The used membrane was rinsed with 0.1 M NaOH, 100 ppm NaOCl for 30 minutes and then flushed with distilled water and stored in 10% ethanol /water (v/v) solution in the refrigerator. The water permeability of the membrane was measured before each run and was found to be similar to that measured for the freshly used membrane. Preparation of Standard Solutions Polynuclear aromatic hydrocarbons (PA) standard mixture (1.0 ppb) was prepared from the stock standard mixture (2000 ppm) by two successive dilutions. First, 5.0 L of the stock mixture was diluted to 750.0 mL with de-ionized water (this is called the working solution). Then, 75.0 L of working solution was diluted to 1000.0 mL with de-ionized water to obtain the desired final concentration. The concentration of each PAH component was then determined accurately from their corresponding calibration curves. Liquid-Liquid Extraction PAHs were extracted from aqueous solution by solvent extraction with dichloromethane. Two 500 ml volumetric flasks were filled to the mark, one with the contaminated water and the other with the treated water (permeate). Each entire sample was poured into a 500 ml separatory funnel and then extracted 5 times in dichloromethane with 10 ml increments each time. Sodium sulfate anhydrous was added to the extractant to ensure the complete removal of water molecules. The drying agent was then removed by filtration in pre- weighed 100 ml round bottomed flasks. After extraction, the two samples were concentrated in a rotary evaporator but not to dryness, then weighed again to the nearest 0.1 mg. The volume left was calculated and adjusted to a final volume of 1 ml. Analysis The analysis of the different samples was carried out with a Varian CP 3800 gas chromatograph coupled with Saturn 2000 MS/MS, a ComboiPal headspace, and SPME autosampler. The column used was a CP Sil 8 CB type column (30 m x 0.25 mm x 0.25 df). Ultrapure helium was used as a carrier gas. The frit sample traps 5 ml of the sample and then purged it for 12 min. The GC-MS instrument was calibrated with the PAH standard mixtures at different concentrations. The column temperature was set at 70 ºC for 1.50 min, then programmed to 200 ºC at 10.0 ºC/min, followed by increase to 270 ºC at a rate of 5.0 ºC/min and finally to 300 ºC at 10.0 ºC/min. The column was then held for 10.0 min with a total run time of 41.50 min. The sample analysis began with injecting of 2L of the extract to MICELLAR ENHANCED ULTRAFILTRATION 41 the splitless injection port of the instrument and then processing the sample with the output referred to the appropriate calibration curve. 3. Results and Discussion 3.1 Micellar enhanced ultrafiltration of a standard aqueous PAH Mixture GC-MS analysis was carried out for a prepared standard PAH mixture containing 16 compounds. The retention time for each component was measured and reported in Table 1. Standard injections were performed with four different concentrations (1, 2, 5, and 10 ppb) for each PAH component in the mixture to obtain different calibration curves. A typical calibration curve is shown in Figure 1 for anthracene. Peak size/10 6 Figure 1. Calibration curve for anthracene in the standard PAH mixture. The micellar enhanced ultrafiltration process was then applied to treat a synthetic aqueous solution containing the standard PAH mixture using an edible nonionic surfactant (Tween 80). Table 1. Retention times Rt (in minutes) for the different components in the standard PAH mixture. No. Compound name Rt No. Compound name Rt 1 Naphthalene 7.693 9 Benz(a)anthracene 24.648 2 Acenaphthylene 11.389 10 Chrysene 24.793 3 Acenaphthene 11.812 11 Benzo(j)fluoranthene 29.025 4 Fluorene 13.052 12 Benzo(k)fluoranthene 29.025 5 Phenenthrene 15.399 13 Benzo(a)pyrene 30.099 6 Anthracene 15.535 14 Indeno(1,2,3-cd)pyrene 33.370 7 Fluoranthene 19.063 15 Dibenz(a,h)anthracene 33.497 8 Pyrene 19.841 16 Benzo(ghi)perylene 34.218 MOHAMED AOUDIA et al. 41 P e a k a re a Obviously, there is a legitimate concern over adding surfactant to the contaminated water at surfactant concentration above its CMC and possibly trading one contaminant (PAH) with another (surfactant). To allay this concern, the use of a food additive surfactant may be a relatively safe approach (Schick, 1967). GC-MS analysis was carried out to estimate the concentration of each PAH solute in permeate, using the appropriate calibration curve. The total ion chromatogram obtained is shown in Figure 2 (green), along the total ion chromatogram for a similar non-treated synthetic aqueous standard PAHs mixture (red). Interestingly, all chromatogram peaks were significantly removed by MEUF treatment. This finding strongly suggests that MEUF by Tween 80 surfactant micelles may be very efficient in removing PAH solutes from the aqueous stream. Figure 2 was used along the PAH calibration curves (determined for standard PAH mixture) to estimate the rejection percent (R%) of each PAH component in the mixture from the following expression where C0 is the initial concentration of the PAH solute in the feed standard mixture and Cp is the permeate solute concentration. R% = [1 – (Cp/C0)] (1) Figure 2. Overlaid total ion chromatogram of a non-treated standard PAH mixture (red) and a MEUF treated standard PAH mixture (green) using Tween 80 (10 × CMC). The detection limit of our GC-MS system used in this study is  0.010 ppb. Thus, we decided to calculate a lower limit for the rejection percent by using a residual solute concentration equal to 0.010 ppb for those PAHs that are completely removed. Such lower limits are reported in Table 2. Time (minutes) MICELLAR ENHANCED ULTRAFILTRATION 41 Table 2. Removal of PAHs from a standard mixture of PAHs by MEUF using Tween 80 (10 × CMC). No. Compound name Co (ppb) Cp ± (0.010 ppb) R% 1 Naphthalene 0.799 Ud* 98.7 2 Acenaphthyle 0.810 Ud 98.8 3 Acenaphthene 0.747 Ud 98.7 4 Fluorene 0.918 Ud 98.9 5 Phenenthrene 1.033 Ud 99.0 6 Anthracene 1.018 Ud 99.0 7 Fluoranthene 0.902 Ud 98.9 8 Pyrene 0.890 Ud 98.9 9 Benz(a)anthracene 0.873 Ud 98.9 10 Chrysene 0.872 Ud 98.9 11 Benzo(j)fluoranthene 0.784 Ud 98.7 12 Benzo(k)fluoranthene 0.844 Ud 98.8 13 Benzo(a)pyrene 0.842 Ud 98.8 14 Indeno(1,2,3-cd)pyrene 0.740 Ud 98.7 15 Benzo(ghi)perylene 0.915 Ud 98.9 16 Dibenz(a,h)anthracene 0.960 Ud 98.9 *Ud: Undetected As clearly seen in this Table, high PAHs rejections (> 98.7 %) were obtained for all components, reflecting a significant efficiency of MEUF in removing PAHs from water. Interestingly, because of the relatively low PAH concentrations used in our experiments (0.800-1.000 ppb), these high PAH rejections show that MEUF is very effective at low solute concentration, an evident advantage over conventional separation techniques such as liquid-liquid extraction, adsorption onto activated carbon or distillation. It is worth noting that aromatic hydrocarbons have a great tendency to adsorb at surfaces and as a result, part of the rejection of aromatics may also be attributed to the solute-membrane interaction in addition to their solubility enhancements in the presence of Tween 80 micelles. It is well recognized that PAHs are very hydrophobic and have a very low solubility in water. For instance, their solubility ranges from 32.5 ppm for naphthalene to 0.14 ppm for pyrene. The recommended total PAH concentration in drinking water supply is 0. 20 ppb whereas that of their most toxic member benzo(a)pyrene is 0.010 ppb (Omani Standards for Unbottled Drinking Water No. 245/1993). It is, therefore, very essential to reduce the concentration of these hazardous chemicals present in contaminated water to 0.20 ppb total concentration as well as to less than 0.010 ppb for the most toxic ones. Indeed, in the range of PAH concentration investigated in this study (0.800-1.000 ppb), the residual concentration of each solute present in the standard PAHs mixture was reduced to  0.010 ppb, in accord with the Oman standards for unbottled drinking water. In fact, several water treatment methods can be used in order to go from high contaminant concentrations to low, but few effective methods can go from minute concentrations to nil or extremely low concentration. MEUF is one of these methods in which efficiency increases as the contaminant concentration decreases. At this juncture, it is worth noting that in diesel contaminated underground water (a real concern for the Sultanate of Oman), PAHs are generally found in mixtures with a considerable number of organic compounds with different: i) structure, ii) hydophobicity, iii) solubility locus in micelle, and iv) micelle-solute interaction. One may, therefore, assume that hydrocarbons with similar hydrophobicity and/or hydrophilicity could compete for a similar site of solubilization in the micelle. This mutual competitive solubilization within the micelle will certainly influence the solubilization behavior of the PAHs (and therefore their rejection by MEUF) in real diesel MOHAMED AOUDIA et al. 02 contaminated underground water relatively to their solubilization in PAH standard mixtures. To elucidate this important issue, we investigated the rejection of PAHs from diesed contaminated water in Oman (Rustaq) by MEUF using Tween 80 surfactant. 3.2 Micellar enhanced ultrafiltration of PAHs from diesel contaminated underground water (Rustaq) MEUF was applied to treat diesel contaminated underground water (Rustaq, Oman) using Tween 80. Total ion chromatograms are shown in Figure 3. The red chromatogram corresponds to the non treated contaminated water sample, whereas the green chromatogram corresponds to the contaminated underground water sample treated by MEUF. All the identified PAH components in the non-treated contaminated water are shown in Table 3 and their concentrations were estimated from their calibration curves. Total ion chromatogram (Figures 3) was used along the calibration curve for each identified PAH to calculate the displayed percent rejection R% in Table 3. Figure 3 shows that, within the experimental error, our results indicate a complete removal of the identified PAH components from the diesel contaminated water sample, indicating that MEUF is also a very effective separation technique in the seldom-investigated extremely low pollutant concentration condition (less than 1 ppb). Table 3. Removal of identified PAH diesel components from contaminated underground water (Rustaq) by MEUF using Tween 80 ( 10 × CMC). No Compound name Co (ppb) Cp ±0.010 ppb R% 1 Naphthalene 0.413 Ud* 97.6 2 2-Methylnaphthalene 0.419 Ud 97.6 3 2,6-dinitrotoluene 0.655 Ud 97.5 4 Dibenzofuran 0.353 Ud 97.2 5 Azobenzene 0.452 Ud 92.3 6 Phenanthrene 0.537 Ud 98.1 7 Anthracene 0.533 Ud 98.1 8 Carbazole 0.792 Ud 98.7 9 Flouranthene 0.482 Ud 97.9 1 0 Pyrene 0.527 Ud 98.1 11 Benz(a) anthracene 0.741 Ud 98.7 12 Chrysene 0.733 Ud 98.6 13 Benzo(b)flouranthene 0.801 Ud 98.8 14 Benzo(k) fluoranthene 0.801 Ud 98.8 15 Benzo(a) pyrene 0.814 Ud 98.8 16 Benzo(ghi) perylene 1.031 Ud 99.0 *Ud: Undetected In treating contaminated water for drinking purpose by MEUF, the main criteria (among others) imposed on the choice of surfactant is related to safety and toxicity considerations. As previously discussed, MEUF treated water will inevitably contain surfactant at a concentration equal or slightly less than the surfactant CMC (Fillipi et al. 1999). The CMC of Tween 80 is 1.2 × 10 –5 M (Aoudia and Al-Shuaily, 2006). This corresponds to a permeate surfactant concentration equal to 15.72 mg/L. Toxicity and carcinogenecity studies of Tween 80 indicated a recommended concentration acceptable for safe human daily consumption not exceeding 25 mg/kg of body weight. An average person can therefore ingest up to 1750 mg of Tween 80 daily without any potential danger, thereby the concentration of Tween 80 in the treated water is extremely low and safe. MICELLAR ENHANCED ULTRAFILTRATION 04 P e a k a re a 4. Conclusion Micellar enhanced ultrafiltration (MEUF) was applied to the treatment of a standard polycyclic aromatic hydrocarbon (PAH) mixture containing 16 components and a diesel contaminated underground water in Rustaq (Sultanate of Oman), using an edible nonionic surfactant (Tween 80). Gas-chromatography-mass spectrometry (GC-MS) was carried out to analyze solutes in the treated and untreated aqueous stream. Within the experimental error ( 0.01 ppb), the totality of PAH compounds in the standard mixture were substantially removed (% R > 98.1%), even though their initial concentrations in standard mixture were extremely low (< 1 ppb). This interesting result suggests that toxic solutes can be effectively removed by MEUF at extremely low concentration. Likewise, the concentration of the totality of PAH identified in the diesel contaminated underground water was reduced to less than  0.01 ppb by MEUF using Tween 80 surfactant, which is below the recommended PAH concentration in drinking water supply for the most toxic member of the PAH group (benzo(a)pyrene,  0.010 ppb). The permeate surfactant concentration is 15.72 mg/L which corresponds to much less than the recommended maximum safe human daily consumption of 1750 mg of Tween 80. Figure 3. Overlaid total ion chromatogram of a non treated contaminated underground water sample from Rustaq (red) and a similar underground water sample treated by MEUF (green) using Tween 80 (10 × CMC). Time (minutes) MOHAMED AOUDIA et al. 00 5. Acknowledgment We thank Sultan Qaboos University, Sultanate of Oman for the financial support (Grant: IG/ SCI / CHEM / 03 /03). 6. References AOUDIA, M., ALLAL, N., DJENNET, H., TOUMI, L., 2003. Dynamic Micellar Enhanced Ultrafiltration: Use of Anionic (SDS)-nonionic (NPE) System to Remove Cr 3+ at Low Surfactant Concentration, J. Membr. Sci, 217: 181-192. AOUDIA, M., Al-SHAAILI. M., 2006. Solubilization of Naphthalene and Pyrene by Sodium Dodecyl Sulfate (SDS) and Polyoxyethylenesorbitan Monooleate (Tween 80) Mixed Micelles, Colloids and Surfaces A: Physicochem. Eng. Aspects, 287: 44-50. AOUDIA, M., AL-HADDABI, B., AL-HARTHI, Z., AL-RUBKHI, A., 2010. 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