Departments of 1Medicine and 2Pathology, Sultan Qaboos University Hospital; 3Department of Medicine, College of Medicine & Health Sciences, Sultan Qaboos University, Muscat, Oman *Corresponding Author e-mail: drsaifak@yahoo.com جناح العالج اإلنقاذي بإأليكوليزوماب لرفض كلوي حاد ومقاوم متعلق بتفاعل جسم مناعي مضاد �صيف خان، داوود الريامي، يا�رض املالعبد، �صجى حممد، مروة الريامي، نبيل اللواتيا abstract: Antibody-mediated rejection (ABMR) jeopardises short- and long-term transplant survival and remains a challenge in the field of organ transplantation. We report the first use of the anticomplement agent eculizumab in Oman in the treatment of a 61-year-old female patient with ABMR following a living unrelated kidney transplant. The patient was admitted to the Sultan Qaboos University Hospital in Muscat, Oman, in 2013 on the eighth day post-transplantation with serum creatinine (Cr) levels of 400 µmol/L which continued to rise, necessitating haemodialysis. A biopsy indicated ABMR with acute cellular rejection. No improvement was observed following standard ABMR treatment and she continued to require dialysis. Five doses of eculizumab were administered over six weeks with a subsequent dramatic improvement in renal function. The patient became dialysis-free with serum Cr levels of 119 µmol/L within four months. This case report indicates that eculizumab is a promising agent in the treatment of ABMR. Keywords: Kidney Transplantation; Transplantation Rejection; Complement Activation; Eculizumab; Case Report; Oman. امللخ�ص: يهدد رف�ض زرع الكلى احلاد بوا�صطة اجل�صم امل�صاد بقاء الزرعه لفرتات ق�صرية وطويلة االأجل ويبقى من التحديات يف جمال زرع االأع�صاء. هذا تقرير حالة عن اأول ا�صتخدام لعقار االإيكوليزوماب يف عمان يف عالج مري�صة تبلغ من العمر 61 عاما حدث لها رف�ض حاد بعد زرع الكلى من متربع حي من غري االأقارب. مت ترقيد املري�ض يف م�صت�صفى جامعة ال�صلطان قابو�ض يف م�صقط، عمان، بتاريخ 2013 يف اليوم الثامن بعد الزراعه حيث و�صل معدل كرياتينني الدم ايل 400 ميكرومول/لرت م�صتمرا يف االأرتفاع حتي و�صل ايل قيم حتتاج الغ�صيل الكلوي. واأ�صارت خزعة الكلي ايل وجود رف�ض خلوي حاد باجل�صم امل�صاد. مل يوجد اأي حت�صن ملحوظ بعد العالج بالطرق القيا�صية املعتادة، واأ�صتمرت احلاجه ايل الغ�صيل الكلوى. اعطيت املري�صة خم�ض جرعات من عقار االإيكوليزوماب على مدى �صتة اأ�صابيع مع حدوث حت�صن كبري الحق يف وظيفة الكلى. و�صل معدل كرياتينني الدم ايل 119 ميكرومول/لرت يف غ�صون خم�صة اأ�صابيع ومل يحتاج املري�ض ايل غ�صيل الكلى بعد ذلك. ي�صري هذا التقرير ايل اأن عقاراالإيكوليزوماب واعد يف عالج رف�ض زرع الكلي اخللوي احلاد بوا�صطة اجل�صم امل�صاد. كلمات مفتاحية: زراعة الكلى؛ رف�ض الزرعه؛ تن�صيط الكومبليمنت؛ عقار االإيكوليزوماب؛ تقريرحالة؛ ُعمان. Successful Salvage Treatment of Resistant Acute Antibody-Mediated Kidney Transplant Rejection with Eculizumab *Saif A. Khan,1 Dawood Al-Riyami,1 Yasser W. Al-Mula Abed,1 Saja Mohammed,1 Marwa Al-Riyami,2 Nabil M. Al-Lawati3 Sultan Qaboos University Med J, August 2016, Vol. 16, Iss. 3, pp. e371–374, Epub. 19 Aug 16 Submitted 29 Apr 15 Revisions Req. 16 Jun & 14 Dec 15; Revisions Recd. 24 Aug 15 & 9 May 16 Accepted 2 Jun 16 doi: 10.18295/squmj.2016.16.03.020 case report Many advances have been made inthe last 50 years in the field of kidney transplantation due to improved under- standing of the role of the immune system in allograft rejection. Additionally, the introduction of powerful immunosuppressive agents like calcineurin inhibitors has significantly reduced acute cellular rejection rates and substantially improved one- year transplant survival rates.1 Despite this, organ rejection is still a major obstacle and long-term post- transplantation outcomes (≥10 years) have not shown much improvement.2 Hyper acute rejection caused by preformed donor- specific antibodies (DSAs) is now extremely rare due to the universal implementation of pre-transplant cross- matching. Antibody-mediated rejection (ABMR) has proven to play a critical role in the short- and long- term survival of kidney allografts. When untreated or insufficiently treated, ABMR may lead to chronic changes that ultimately result in graft loss.3 This case report demonstrates the successful recovery of renal function due to the administration of eculizumab in a patient with ABMR following a living unrelated transplant. Case Report A 61-year-old Omani woman with end-stage renal disease due to hypertension was admitted to the Sultan Qaboos University Hospital in 2013 eight days post- Successful Salvage Treatment of Resistant Acute Antibody-Mediated Kidney Transplant Rejection with Eculizumab e372 | SQU Medical Journal, August 2016, Volume 16, Issue 3 transplantation. She had no history of spontaneous abortions or venous thromboem-bolisms. She had been on regular haemodialysis at a local dialysis centre for three years before undergoing a living unrelated kidney transplant abroad. Unfor-tunately, details regarding tissue typing, cross-matching, immune suppression induction and the operative procedure were unavailable. At presentation, she had serum creatinine levels of >400 µmol/L which increased to 607 µmol/L a few days later. Ultrasonography of the transplanted kidney showed a normal-sized graft with a resistive index of 0.91. Four days after admission, on the 12th post- operative day, an ultrasound-guided renal transplant biopsy showed glomerular thrombosis, mild peritubular capillaritis, interstitial haemorrhage, strong complement component 4 (C4) d positivity in the peritubular capillaries, mild to moderate focal lymphocytic tubulitis and mild interstitial inflamm- ation [Figure 1]. The arteries and arterioles were normal. These findings were consistent with acute class II ABMR and T cell-mediated acute cellular rejection (ACR). The patient was treated with 250 mg of intravenous methylprednisolone for three days, 1.5 mg/kg of rabbit anti-thymocyte globulin for a total cumulative dose of 980 mg, 2 g/kg of intravenous immunoglobulin (IVIG) and five cycles of plasma exchange (PLEX) at 2.5 L per exchange. She also received maintenance immunosuppression with oral prednisolone, tacrolimus and mycophenolate. The patient showed no improvement in renal function and remained dialysis-dependent after four weeks of conventional treatment. A second biopsy was performed on the 32nd day post-transplantation which indicated on-going ABMR (acute thrombotic microangiopathy, peritubular capillaritis and strong C4d positivity) and features of chronic ABMR, but no evidence of significant ACR. Intimal fibrosis was seen in the arteries as the result of an earlier acute rejection. These findings were believed to indicate resistance to conventional treatment; as a result, the decision was made to proceed with eculizumab administration. Other treatment options such as rituximab and bortezomib were not considered in view of the strong C4d staining in the peritubular capillaries and persistent findings of ABMR indicative of complement pathway activation. The patient received a meningococcal vaccine two weeks prior to the initiation of the eculizumab treatment as per the manufacturers’ recommendations. She received a total of five doses of eculizumab, with the first four doses of 900 mg administered on a weekly basis and the fifth dose of 1,200 mg given two weeks after the fourth dose. A dramatic response in renal function was observed following the first dose [Figure 2]. Her serum creatinine levels continued to improve and had decreased to 119 µmol/L four months after the eculizumab treatment. Discussion ABMR occurs in 5–7% of all renal transplants and 20–48% of pre-sensitised positively cross-matched patients.4 Graft dysfunction usually manifests within a few days of the transplant procedure; however, allograft dysfunction with a resultant increase in serum creatinine may not be present in all cases.4 DSAs play a major role in transplant rejection; Figure 1A‒C: A: Haematoxylin and eosin stain at x200 magnification showing an area of interstitial haemorrhage with acute inflammation. B: Jones’ stain at x400 magnification showing glomerular thrombosis with intraluminal neutrophils. C: Complement component 4d stain at x200 magnification showing strong diffuse positive staining. Figure 2: Serum creatinine levels in a 61-year-old Omani woman with antibody-mediated rejection following a kidney transplant. The patient showed no improvement after six weeks of conventional therapy and was treated with five doses of eculizumab; she became dialysis-free after the first dose. The dotted lines represent the timing of the first and final doses of eculizumab, respectively. Saif A. Khan, Dawood Al-Riyami, Yasser W. Al-Mula Abed, Saja Mohammed, Marwa Al-Riyami and Nabil M. Al-Lawati Case Report | e373 (10‒50%) and minimal (<10%) staining.8 The goal of ABMR treatment is the reduction and removal of DSAs and the elimination of the B cell and plasma cell population responsible for the production of these antibodies. The international guidelines do not outline any evidence-based treatments for acute ABMR.11 Historically, IVIG and PLEX therapy have been used as first-line treatments for ABMR.11 These therapies are difficult to assess because treatment options, doses and regimens are not standardised. Moreover, no randomised control studies have supported the use of IVIG in acute ABMR despite its common utilisation in this context.12 Although the role of PLEX has not been confirmed, studies have shown it to be beneficial especially in combination with other agents.13,14 Other strategies for managing ABMR include the use of rituximab, which targets pre-B cells and B cells, and bortezomib, which is a proteasome inhibitor.15,16 Uncontrolled randomised studies have shown benefit in both rituximab and bortezomib.11,17 These agents are usually used in conjunction with IVIG and/or PLEX.11 Eculizumab is a humanised monoclonal antibody directed against complement protein-C and has recently been used as a new therapeutic agent for ABMR with emerging data supporting its applicability and efficacy in kidney transplantation.11,18 It is a term- inal complement inhibitor initially approved for the management of paroxysmal nocturnal haemoglob- inuria and atypical haemolytic uremic syndrome.19,20 It targets complement component 5, thus blocking the complement cascade and the formation of the complement membrane attack complex.21 To the best of the authors’ knowledge, the present case report is the first to detail the use of eculizumab to treat ABMR in Oman. In this case, eculizumab was administered after a failed response to conventional treatment (IVIG, PLEX, steroids and anti-thymocyte globulin) and histological evidence of persistent ABMR on a repeated graft biopsy four weeks post-transplantation. The patient exhibited a dramatic improvement in renal function following five doses of eculizumab. González-Roncero et al. confirmed the efficacy of this agent in the treatment of two cases of ABMR, especially when the biopsy shows C4d staining.22 Several other reports also endorse the use of eculizumab in the treatment of ABMR.23,24 However, these findings need to be confirmed by randomised control trials. The current case report highlights a common dilemma arising from commercial transplants, particularly as these surgeries may be carried out without optimal preparation and with limited peri-operative information available following the procedure. they are either preformed or develop de novo after transplantation.5 These antibodies are most commonly directed against the human leukocyte antigen (HLA) or major histocompatibility complex (MHC) class I and II. The former is expressed in all nucleated cells while the latter is restricted to endothelial cells and antigen-presenting cells like dendritic cells and B-lymphocytes. The antibodies can also be directed against other DSAs/non-HLA-like platelets, specific antigens, renin-angiotensin pathways and MHC class I-related chain A. Previous transplantations, pregnancy and blood transfusions are major risk factors leading to sensitisation against MHC.6 Anti-MHC antibodies either result in direct injury to the capillary endothelium or indirect injury via complement activation which is the major mechanism leading to the formation of a membrane attack complex. This formation results in cell injury and manifests histologically as glomerulitis, peritubular capillaritis and vascular endothelial injury. Endothelial damage also causes platelet activation and microthrombus formation. The Banff Foundation for Allograft Pathology has proposed that at least two of the following criteria indicate a diagnosis of ABMR: (1) the presence of DSAs; (2) a positive C4d stain; and (3) histological evidence of glomerulitis, peritubular capillaritis and vascular endothelial injury.7,8 In the present case, two of the above criteria were met. However, the patient was not tested for panel-reactive antibodies or DSAs; the latter was due to the unavailability of the technique at that time in Oman. However, it is an established practice to initiate treatment of ABMR in circumstances where the criteria are not entirely fulfilled due to multiple factors, including an inability to measure DSAs due to the presence of non-HLA antibodies or the possibility of DSA adsorption into the allograft.9 On the other hand, C4d stains may be falsely negative in areas of necrosis or with less sensitive immunohistochemistry techniques. Protocol biopsies have also demonstrated C4d variability in staining over time from positive to negative.8 The complement split product C4d results from the breakdown of C4b into C4d and C4c. It has a high affinity for endothelial and basement mem- branes and is expressed in the normal kidney mesangium and vascular pool owing to the constant complement turnover.10 In cases of immune-mediated glomerulopathy, it extends into the glomerular capill- aries and deposits in the peritubular capillaries in a transplanted kidney. Immunohistochemistry and immunofluorescence are two methods to detect C4d, the latter of which is more sensitive. Diffuse staining of C4d (more than 50% of a peritubular capillary stain) is highly suggestive of ABMR, in comparison to focal Successful Salvage Treatment of Resistant Acute Antibody-Mediated Kidney Transplant Rejection with Eculizumab e374 | SQU Medical Journal, August 2016, Volume 16, Issue 3 Conclusion ABMR is a serious complication that can occur following organ transplantation. It can have a grave impact on long-term kidney transplant survival. Several non-evidence-based strategies exist to treat ABMR. 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