C:\Users\UNIVERSA MEDICINA\Docu 42 *Clinical Pathology Department, Medical Faculty, Jenderal Soedirman University **Bioethics and Humaniora Department, Medical Faculty, Jenderal Soedirman University Correspondence: Vitasari Indriani Clinical Pathology Department, Medical Faculty, Jenderal Soedirman University Gumbreg Street No 1, Purwokerto, Central Java Indonesia Email: vita.indriani@gmail.com ORCID ID: 0000- 0001-8563-1781 Date of first submission, December 11, 2 01 8 Date of final revised submission, March 31, 2020 Date of acceptance, April 1, 2020 This open access article is distributed under a Creative Commons Attribution- Non Commercial-Share Alike 4.0 International License ABSTRACT UNIVERSA MEDICINA Duration of diabetes as an important risk factor of microalbuminuria in type 2 diabetes Vitasari Indriani*, Tri Lestari*, and Vidya Dewantari** BACKGROUND Microalbuminuria is the earliest evidence of diabetic nephropathy and a major predictor of end stage renal disease (ESRD). The objective of the study was to determine the influence of several risk factors on the presence of microalbuminuria in type 2 diabetics. METHODS This observational cross-sectional study was done on 73 patients with type 2 diabetes, who attended the Prolanis program in Primary Health Care from May to November 2018. Detailed medical histories including duration of diabetes and relevant clinical examinations including fasting blood sugar (FBS), post-prandial blood sugar (PPBS), HbA1c, serum creatinine, blood urea and urinary microalbumin were recorded for each patient. A multiple regression analysis was used to analyze the data. The analysis was assessed at 5% level of significance. RESULTS Mean age of study population was 51.89 ± 6.78 years with female preponderance (51.1%). Mean FBS, PPBS, HbA1c, duration of diabetes, systolic blood pressure, microalbuminuria and serum creatinine was 182.51 ± 74.63 mg/dL, 186.25 ± 26.72 mg/dL, 8.8 ± 1.83%, 9.37 ± 5.96 years, 118.44 ± 4.13 mmHg, 30.32 ± 3.2 mg/day and 1.33 ± 0.64 mg/dL respectively. Duration of diabetes and HbA1c were positively correlated with microalbuminuria (β=0.052; Beta =0.367; p<0.001 and β=0.058; Beta=0.363; p<0.001) respectively. CONCLUSIONS Duration of diabetes was the most important risk factor of microalbuminuria in type 2 diabetes patients. Therefore microalbuminuria can predict diabetic nephropathy earlier, as a warning to prevent further worsening of diabetic complications. Keywords: Diabetic nephropathy, HbA1c, microalbuminuria, type 2 diabetes ORIGINAL ARTICLE pISSN: 1907-3062 / eISSN: 2407-2230 DOI: http://dx.doi.org/10.18051/UnivMed.2020.v39.42-46 C it e t h is ar t ic le a s: In d ri a ni V, Lestari T, Dewantari V. Duration of d ia betes as a n import an t ri sk factor of microalbuminuria in type 2 diabetes. Univ Med 2020;39:42- 6. d oi : 10.18051/UnivMed.2020. v38 .42-46. January-April, 2020 Vol.39- No.1 43 INTRODUCTION Diabetes mellitus (DM) is a metabolic disorder characterized by persistent hyperglycemia resulting from defects in insulin secretion, action or both. Chronic hyperglycemia initiates microvascular complications including nephropathy, retinopathy and neuropathy. Poor glycemic control plays an important role in the development and progression of nephropathy with associated increase in morbidity and mortality. (1) The presence of albuminuria is an indicator of renal impairment. Microalbuminuria, defined as an elevation of urinary albumin excretion in the range of 30-300 mg/24 hr or 20-200 µg/min, is associated with adverse health outcomes in adults. (2) Microalbuminuria is strongly associated with endothelial dysfunction, which increases the risk of nephropathy and other complications in diabetes. (3) Glycosylated hemoglobin (HbA1c) has been used as a measure of long-term glycemic control and has proven to be a more accurate and stable measure than fasting blood sugar level (FBS) and post-prandial blood sugar (PPBS).(4)An annual assessment of kidney function by the determination of urinary albumin excretion and of HbA1c for glycemic control is recommended for patients with diabetes.(5) Chen et al. (6) identified the relationships of high normal albuminuria and glycemic control on microalbuminuria development among type 2 diabetes patients. Previous studies had revealed that various factors such as glucose level(7), glycemic variability(8) and insulin resistance impact development of microalbuminuria in type 2 diabetes. (9) In the present study the population comprised patients with type 2 diabetes mellitus attending the primary health care system under the chronic disease management program (Prolanis). Only FBS and PPBS were checked every month. Since increased risk of nephropathy must be detected earlier, further correlations between the various factors were investigated in our study. The present study was performed to determine the relationship of HbA1c with microalbuminuria by considering the following factors: age, gender, FBS, PPBS, blood pressure, blood urea level, serum creatinine level and duration of diabetes. METHODS Research design An observational study of cross-sectional design was done in the Prolanis program in Primary Health Care (FKTP Klinik Tanjung Purwokerto) from May 2018 to November 2018. Research subjects An observational study and a randomized sample of 73 clinically diagnosed cases of type 2 diabetes aged >18 years. Based on a previous finding that serum creatinine was positively correlated with microalbuminuria (r=0.33) (10) and using =0.05, =0.20, and drop-out rate of 20%, the total calculated sample was 73 subjects Thirty seven female and thirty six male clinically diagnosed cases of type 2 diabetes who attended the Prolanis program were included. Patients suffering from acute and chronic inflammatory conditions, pre-existing chronic kidney disease, glomerulonephritis, nephrotic syndrome, smokers, alcoholics, patients on nephrotoxic drugs, and primary hypertensives were excluded from the study. Medical records of the patients comprising age, gender, duration of diabetes, glycated hemoglobin, blood urea, serum creatinine, and urinary microalbumin levels were analyzed for each patient. Laboratory analysis Blood samples were collected after 10-12h of fasting. Taking all aseptic and antiseptic precautions, 5 mL of blood was drawn from the antecubital vein and a 24-hour urine sample was collected in a sterilized container. The following measurements were done: FBS, PPBS, blood urea, serum creatinine by enzymatic method (Dimension EXL 200), HbA1c measured by Clover A1c Self with boronate affinity method, and microalbuminuria by fluorescent immunoassay (FIA) method (Ichroma Microalbumin). Statistical analysis All analyses were performed using IBM SPSS version 20. Results on continuous measurements were presented as mean ± standard deviation (SD) and results on categorical measurements as numbers (%). A determine the relationship between several risk factors and microalbuminuria. The analysis was assessed at 5% level of significance. 44 Table 1. Distribution of characteristics and laboratory parameters of patients with type 2 diabetes *Data presented as Mean ± SD, except age in % Ethical clearance The study has been approved by the Health Research Ethics Commission (Komisi Etik Penelitian Kesehatan, KEPK), Medical Faculty, Jenderal Soedirman University, under no. 1792/KEPK/IV/2018. All subjects were informed of the study protocol in written and verbalform and provided informed consent. RESULTS The mean age of the subjects was 51.89 ± 6.78 years, and most subjects were female (51.1%). The mean serum creatinine was 1.33 ± 0.64 mg/dL, mean duration of diabetes was 9.37 ± 5.96 years, mean HbA1c was 8.8 ± 1.83 (Table 1). DISCUSSION Our study showed that duration of diabetes was the most potential risk factor of microalbuminuria. A similar result was shown by a study involving 227 subjects with type 2 DM in Kathmandu, in that diabetes duration was the most important risk factor for the development of microalbuminuria in diabetes. (11) Another study showed consistent results in that duration of diabetes was an independent risk factor for microalbuminuria.(12) Microalbuminuria is the most sensitive marker of early recognition of diabetic nephropathy. Monitoring microalbuminuria and other risk factors associated with this condition is important to take measures to prevent or postpone overt nephropathy.(13) More importantly, tight glycemic control with insulin during the microalbuminuria stage has been shown to prevent the development of overt diabetic nephropathy.(14) The level of HbA1c has been widely accepted as an indicator of mean daily blood glucose concentration over the preceding 8-12 weeks. Our study also found a significant relationship between HbA1c and microalbuminuria. Bhoomika et al. (15) studied risk factors for microalbuminuria in type 2 diabetes mellitus patients and reported significantly high HbA1c levels in patients with microalbuminuria. Another study also highlights that there was a significant correlation between microalbumin levels and HbA1c in diabetic type 2 subjects. (16) However, another study in Iran did not find a significant association between HbA1c and microalbuminuria.(12) Table 2. Multiple linear regression analysis of several risk factors and microalbuminuria Beta p value Age (years) 0.946 0.086 0.033 Gender 0.876 0.112 0.064 Systolic blood pressure (mmHg) 0.270 0.081 0.034 Diastolic blood pressure (mmHg) 0.278 0.085 0.043 Diabetes duration (years) 0.052 0.367 <0.001* FBS (mg/dL) 0.045 0.091 0.059 PPBS (mg/dL) 0.48 0.086 0.033 HbA1c (%) 0.058 0.363 <0.001* Blood urea (mg/dL) 0.054 0.087 0.059 Serum creatinine (mg/dL) 0.048 0.076 0.023 Multiple linear regression showed that diabetes duration was the most potential risk factor of microalbuminuria ( Beta=367; p<0.001) followed Beta=0.363; p<0.001) Parameter Diabetic type 2 patients (n=73) Age (yr) Gender (%) Male Female Fasting blood sugar (mg/dL) Post prandial blood sugar (mg/dL) Systolic blood pressure (mmHg) Diastolic blood pressure (mmHg) HbA1c (%) Microalbuminuria (mg/day) Serum creatinine (mg/dL) Blood urea (mg/dL) Duration of diabetes (yr) 51.89 ± 6.78 36 (48.9%) 37(51.1%) 182.51 ± 74.63 186.25 ± 26.72 118.44 ± 4.13 84.44 ± 19.25 8.8 ± 1.83 30.32 ± 3.2 1.33 ± 0.64 43.2 ±15.0 9.37 ± 5.96 45 Relationship of duration of type 2 diabetes mellitus with microalbuminuria and high HbA1c level has been reported. Chronic hyperglycemia is responsible for the pathogenesis of diabetic nephropathy. The possible mechanism is that hyperglycemia leads to glycation of virtually all proteins, resulting in the formation of advanced glycation end products. The interaction of advanced glycation end products and their receptors and increased activity of the polyol pathway have been implicated as mediators of increased microvascular permeability, ischemia and angiogenesis.(16) Baig et al.(17) studied 60 patients and concluded that in patients with type 2 diabetes mellitus, long duration of diabetes and poor glycemic control significantly correlated with high level of microalbumin. Another study reported that higher grades of HbA1c are associated with higher severity of microalbuminuria and so can be used as a marker of good glycemic control.(18) Microalbuminuria usually comes out 5-15 years after the diagnosis of type 2 diabetes mellitus. Combination of glomerular hypertension and glomerular hyperperfusion the remaining nephrons. These changes occur in the absence of uremia. In later stages the glomerular filtration rate (GFR) decreases with the appearance of macroproteinuria along with clinical signs of nephropathy and finally leads to end stage renal disease (ESRD).(19) Liu et al. (3) and Kondaveeti et al.(20) also reported that good glycemic control was the strong influencing factor which played a key role in the transition of non-microalbuminuric subjects to microalbuminuric. Several limitations were encountered in this study. First, the study was not population- based and only patients who presented at diabetes centers were included. This may have introduced referral bias and it would therefore be difficult to extend our findings to the general population of diabetes patients. Second, we have also not examined the role of HbA1c variability on microvascular complication development, and we had difficulties in determining the duration of diabetes. Third, the cross-sectional nature of the study design limits the reliability of the observed associations between risk factors and diabetic nephropathy. Because microalbuminuria is widely used as a sensitive risk marker to identify those at risk for renal dysfunction, screening programs should be implemented at an early stage to prevent or postpone end-stage renal disease (ESRD). So, it may be suggested that determination of microalbumin levels in urine is an easy method of screening diabetic patients, especially diabetic patients with long-term diabetes. Further well-designed prospective studies enrolling larger populations are warranted. 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