Review 78 Urology Journal Vol 6 No 2 Spring 2009 CYP1A1 Polymorphisms and Risk of Prostate Cancer A Meta-analysis Abjal Pasha Shaik,1 Kaiser Jamil,2 Prabhavathy Das2 Introduction: Two common polymorphisms in cytochrome P450; family 1, subfamily A, polypeptide 1 (CYP1A1); have been implicated as a risk factor of prostate cancer, but individual studies have been inconclusive or controversial. We reviewed studies on CYP1A1 polymorphisms in patients with prostate cancer. Materials and Methods: The strategy searching in the PubMed was based on combinations of prostate cancer, CYP1A1, CYP1A1 gene polymorphism, and genetic susceptibility. The last search update was May 2008. The retrieved articles and their bibliographies of were evaluated and reviewed independently by 2 experts. We shortlisted 19 studies, of which 14 on sporadic prostate cancer were analyzed. Overall, 2573 patients with prostate cancer and 2576 controls were analyzed. Results: The random effects odds ratio was 1.350 (95% confidence interval, 1.110 to 1.641; P = .003) for T/C polymorphism and 1.085 (95% confidence interval, 0.863 to 1.364; P = .49) for A/G polymorphism. The A/G polymorphism was not associated with increased risk of prostate cancer. However, the T/C polymorphism showed conflicting results in different studies, while overall, this polymorphism showed significant effects on the susceptibility to prostate cancer. There was no significant between- study heterogeneity for both polymorphisms with respect to distribution of alleles. Conclusion: This meta-analysis suggests that while the CYP1A1 T/C polymorphism is likely to considerably increase the risk of sporadic prostate cancer on a wide population basis, the A/G polymorphism may not influence this risk. However, the association of polymorphisms may be significant with respect to smoking history, diet habits, ethnicity, and race. Urol J. 2009;6:78-86. www.uj.unrc.ir Keywords: prostatic neoplasms, meta-analysis, CYP1A1, genetic polymorphisms 1Research Wing, Indo-American Cancer Hospital and Research Centre, Hyderabad, India 2Department of Genetics, Bhagwan Mahavir Medical Research Centre, Masab Tank, Hyderabad, India Corresponding Author: Kaiser Jamil, MD Department of Genetics, Bhagwan Mahavir Medical Research Centre, Masab Tank, Hyderabad-500 004, India Phone: +91 40 6666 2032 E-mail: kaiser.jamil@gmail.com Received July 2008 Accepted November 2008 INTRODUCTION Prostate cancer is one of the most common malignancies in men, and the prostate is the leading site for cancer incidences, accounting for 31% of new cancer cases in men.(1) The incidence of prostate cancer varies greatly with race and geography. In India, the annual mortality in 2000 was 700 000, and the annual estimate of cancer for the year 2001 was 980 000. It is relatively rare for prostate cancer to be diagnosed in men younger than 50 years old, but above this age, the incidence and mortality rates increase exponentially.(1,2) Genetic susceptibility to prostate cancer is an important research area, especially since the incidence of prostate cancer has been rapidly increasing. Prostate cancer susceptibility loci have been reported to be hereditary prostate cancer 1 gene at 1q24, CYP1A1 and Prostate Cancer—Shaik et al Urology Journal Vol 6 No 2 Spring 2009 79 predisposing for prostate cancer gene at 1q42, X-linked hereditary prostate cancer gene at Xq27, capsule biosynthesis protein gene at 1p36, and hereditary prostate cancer 20 gene at 20q13.(2) The association of prostate cancer with polymorphisms of common variants in genes involved in steroid hormone metabolism including androgen receptor (AR), steroid-5- alpha-reductase, alpha polypeptide 2, cytochrome P450 subfamily XVII, vitamin D receptor, etc, have been extensively examined.(3-7) Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) is involved in xenobiotic metabolism and classified as a phase I enzyme. The expression of the CYP1A1 is induced in a ligand-dependent fashion by the aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator.(1,2,8) The CYP1A1 gene plays an important role in carcinogenesis of various cancers, and it might affect carcinogenesis of prostate cancer through alteration of genotoxicity and hormone imbalance. It is inhibited by fluoroquinolones and macrolides, induced by aromatic hydrocarbons. There are 3 main subtypes of CYP1A: M1, M2, and M3. CYP1A1 and CYP1B1 are regulated by the aryl hydrocarbon receptor, a ligand-activated transcription factor which is a part of the phase I reactions in drug metabolism.(8) Current published evidence suggests that both environmental and genetic factors influence the pathogenesis of prostate cancer.(9,10) Polymorphisms of the CYP1A1 may modify the risk for prostate cancer.(8,9) The CYP1A1 gene encodes a phase I cytochrome, P-450 enzyme, that converts environmental procarcinogens to reactive intermediates having carcinogenic effects.(11) In addition, CYP1A1 is involved in the oxidative metabolism of estrogens, which may play a critical role in the etiology of prostate cancer.(12) Two common polymorphisms in CYP1A1 have been reported: one is a T/C substitution located 264 bp downstream from the 3’-flanking region, forming an Msp1 restriction site (CYP1A1m1); the second is a G/A substitution at the 4889 bp position of exon 7, which leads to an amino acid substitution (Ile to Val) of its protein (CYP1A1m2).(1,13) The association of these CYP1A1 single nucleotide polymorphisms (SNPs) with cancer (eg, lung, oesophageal, breast, oral cavity cancers) has been well documented.(1,14) More recently, the association between CYP1A1 SNPs and prostate cancer has been reported in some groups.(13) Molecular epidemiological studies have presented seemingly contradictory results concerning the potential role of the CYP1A1 polymorphisms in prostate cancer susceptibility. Using relevant accumulated data, a quantitative methodology was used to estimate the strength of CYP1A1 genetic associations. MATERIALS AND METHODS Identification of Relevant Studies We considered all studies that examined the association of the CYP1A1 gene polymorphisms with prostate cancer. We shortlisted 19 studies, of which 14 were analyzed further. Results of only sporadic prostate cancer were considered for meta-analyses. We excluded studies with familial linkage designs. All of the obtained studies (familial and sporadic) were tabulated to have an overview of the number of studies carried out in prostate cancer which used CYP1A1 gene for analyses. Search sources included MEDLINE which was searched through PubMed. The last search update was May 2008. The search strategy was based on combinations of prostate cancer, CYP1A1, CYP1A1 gene polymorphism, and genetic susceptibility. The retrieved articles and their bibliographies of were evaluated and reviewed independently by 2 experts. Case-control studies were eligible if they had determined the distribution of CYP1A1 genotypes in prostate cancer cases and in a concurrent control group of prostate cancer-free subjects using a molecular method for genotyping. We accepted disease-free controls regardless of whether they had benign prostatic hyperplasia or did not. Cases with prostate cancer were eligible regardless of whether they had a first-degree relative with prostate cancer or not. However, we excluded hereditary prostate cancer results from 2 family-based studies.(15,16) Data Extraction The following information was sought from CYP1A1 and Prostate Cancer—Shaik et al 80 Urology Journal Vol 6 No 2 Spring 2009 extracted data: authors, journal and year of publication, country of origin, selection and characteristics of prostate cancer cases and controls, demographics, racial descent of the study population, eligible and genotyped cases and controls, and number of cases and controls for each CYP1A1 genotype. Meta-analysis The primary analysis for all CYP1A1 gene polymorphisms was based on distribution of genotypes among various populations, and then, evaluation of the overall differences within them. We also examined the contrast of the two groups of homozygotes (the dominant and recessive). The odds ratio (OR) was used for analyses of results. For each genetic contrast, we estimated the between-study heterogeneity across all eligible comparisons, using the modified chi-square test. Heterogeneity was considered significant is P value was less than .05. All analyses were conducted using the Comprehensive Meta-Analysis Software version 2 (Biostat, Englewood, New Jersey, USA). RESULTS Eligible Studies Fourteen studies probing the relationship between the CYP1A1 gene polymorphism and prostate cancer susceptibility were identified.(15-28) Two studies by Chang and colleagues and Cunningham and coworkers(15,16) also included a family-based history; therefore, the data of only sporadic prostate cancer cases were collected (Table 1). Most of the studies had selected patients with prostate cancer based on a histological diagnosis from biopsy and/or prostatectomy. In 1 study by Nock and coleagues,(17) controls were unaffected brothers of the patients with prostate cancer. Controls did not have a clinical diagnosis of prostate cancer, confirmed using additional screening (with digital rectal examination, prostate specific antigen [PSA < 4 ng/mL], and needle biopsy or prostate resection; Table 1). A few investigators had also matched their groups for smoking status and alcoholism in relation to risk of prostate cancer. Molecular methods for genotyping were checked. All studies had used polymerase chain reaction assay, and 3 studies had also used sequencing. Meta-analysis The selected studies included a total of 5832 subjects (2766 patients and 3066 controls) while the eligible subjects were 2573 patients and 2576 controls. Allele and genotype frequencies per Samples Age of Studied Population, y Study Population Patients Controls Patients Controls Chang et al, 2003(15) Caucasians and African-Americans 159 familial and 245 sporadic 222* Mean, 61.0 for familial and 58.7 for sporadic Mean, 58 Beer et al, 2002(18) Caucasians 117 183 ≥ 18 ≥ 18 Atkas et al, 2004(19) Turkish 100 107† Mean, 68.2 (49 to 86 ) Mean, 67.8 (43 to 87) Mittal and Srivastava, 2007(20) Indian 130 140 Mean, 62.5 Mean, 58.5 Li, 2008(21) Chinese 208 230 Median, 72.0 (46 to 94) Median, 67 (45 to 81) Cunningham et al, 2007(16) Hispanic, Caucasian, and African-American 438 familial and 499 sporadic 493 45 to 89 45 to 89 Yang et al, 2006(22) South Chinese 225 250 Mean, 71.6 Mean, 71.0 Nock et al, 2006(17) Caucasians, African- Americans, and Asians 439 479‡ Mean, 61.5 Mean, 62.8 Silig et al, 2006(23) Turkish 152 169 50 to 85 49 to 88 Caceres et al, 2005(26) Chilean 103 132 Mean, 68.7 Mean, 63.3 Figer et al, 2003(27) Israeli 224 250 Mean, 64.6 (45 to 81) Mean, 61.7 (35 to 83) Murata et al, 2001(28) Japanese 115 204 Mean, 73.0 Mean, 71.2 Suzuki et al, 2003(24) Japanese 81 105 Mean, 70.6 (40 to 88) Mean, 71.2 (51 to 88) Acevedo et al, 2003(25) Chilean 128 102† Mean, 68.6 Mean, 63.4 Table 1. Characteristics of the Study Population in Selected Studies Included in Meta-analyses *Of the controls, 5.6% had brothers or a father affected with prostate cancer. †The controls were men with benign prostatic hyperplasia. ‡The controls were unaffected brothers of the patients with prostate cancer. CYP1A1 and Prostate Cancer—Shaik et al Urology Journal Vol 6 No 2 Spring 2009 81 group are shown in Tables 2 and 3. Some other studies not included in the meta-analysis but found relevant are presented in Table 4.(9,16,17,27-31) The T allele was the most highly represented among controls and cases of all studies irrespective of the descent. Overall, the prevalence of TT, TC, and CC genotypes was 52.6%, 67.7%, and 20.6% in the control individuals and 48.0%, 61.2%, and 13.9% in the patients with prostate cancer. For the Ile/Val polymorphism, the prevalence of AA, AG, and GG genotypes was 66.6%, 26.8%, and 6.4% in the controls and 64.3%, 28.8%, and 6.7% in the patients. The distribution of genotypes in both of the groups was consistent with Hardy- Weinberg equilibrium in all studies. Overall Effects for Alleles The T/C polymorphism was associated with increased risk of prostate cancer (summary random effects OR, 1.350; 95% confidence interval [CI], 1.110 to 1.641; P = .003; Figure 1). No association was found between A/G polymorphism with prostate cancer .The summary random effects OR for G/A polymorphism was 1.085 (95% CI, 0.863 to 1.364; P = .49; Figure 2). However, there was no significant heterogeneity between the 14 study comparisons for both polymorphisms with respect to distribution of alleles. The Q-value for T/C polymorphism was 9.799 (I2 = 28.561; P = .20; Table 5), while for A/G polymorphism, it was 7.968 (I2 = 24.702; P = .24; Table 6). To assess the publication bias among the selected Patients Genotype (MspI) in Studies Prostate Cancer Controls Chang et al, 2003(15) TT 188 (83.9) 135 (75.0) TC 36 (16.1) 39 (21.7) CC 0 6 (3.3) Mittal and Srivastava, 2007(20) TT 55 (42.3) 75 (53.6) TC 69 (53.1) 58 (41.4) CC 6 (4.6) 7 (5.0) Li, 2008(21) TT 78 (37.5) 102 (44.4) TC 100 (48.1) 84 (36.5) CC 30 (14.4) 44 (19.1) Yang et al, 2006(22) TT 76 (33.8) 96 (38.4) TC 116 (51.6) 112 (44.8) CC 33 (14.7) 42 (16.8) Silig et al, 2006(23) TT + TC 142 (94.0) 153 (90.0) CC 10 (6.0) 16 (10.0) Caceres et al, 2005(26) TT 39 (38.2) 74 (56.2) TC 50 (48.0) 47 (35.4) CC 14 (13.8) 11 (8.4) Murata et al, 2001(28) TT 60 (52.2) 118 (59.0) TC 49 (42.6) 74 (37.0) CC 6 (5.2) 8 (4.0) Suzuki et al, 2003(24) TT 46 (35.8) 46 (43.8) TC 39 (48.1) 37 (35.2) CC 13 (16.0) 22 (21) Acevedo et al, 2003(25) TT 39 (38.2) 72 (56.2) TC 49 (48.0) 45 (35.1) CC 14 (13.7) 11 (8.5) Table 2. Distribution of CYP1A1 MspI Polymorphism in Various Populations Patients Genotype (Ile/Val) in Studies Prostate Cancer Controls Chang et al, 2003(15) AA 210 (93.8) 162 (90.0) Ag 14 (16.1) 18 (10.0) gg 0 0 Beer et al, 2002(18) AA 101 (91.8) 129 (88.3) Ag 7 (6.4) 17 (11.6) gg 2 (1.2) 0 Atkas et al, 2004(19) AA 41 (41.0) 50 (46.7) Ag 45 (45.0) 51 (47.7) gg 14 (14.0) 6 (5.6) Li, 2008(21) AA 120 (57.7) 150 (65.2) Ag 75 (36.1) 66 (28.7) gg 13 (6.2) 14 (6.1) Yang et al, 2006(22) AA 113 (50.2) 151 (60.4) Ag 90 (40.0) 86 (34.4) gg 22 (9.8) 13 (5.2) Murata et al, 2001(28) AA 60 (52.2) 125 (62.5) Ag 42 (36.5) 64 (32.0) gg 13 (11.3) 11 (5.5) Suzuki et al, 2003(24) AA 39 (48.1) 65 (61.9) Ag 34 (42.0) 33 (31.4) gg 8 (9.9) 7 (6.7) Table 3. Distribution of CYP1A1 Ile/Val Polymorphism in Various Populations CYP1A1 and Prostate Cancer—Shaik et al 82 Urology Journal Vol 6 No 2 Spring 2009 Samples Age, y Study Population Patients Controls Studied Genotype Patients Controls Interpretation Cunningham et al, 2007(16) Hispanic, Caucasian, and African-American 499 493 SNP analysis 45 to 89 45 to 89 No significant association Nock et al, 2006(17) Asians, Caucasian, and African-American 439 479 CYP1A1 (Ile/val) Mean, 61.5 Mean, 62.8 No significant association Nock et al, 2007(31) Asians, Caucasian, and African-American 637 244 CYP1A1 (Ile/val) Mean, 60.8 Mean, 71.6 No significant association Figer et al, 2003(27) 224 250 CYP1A1 (Ile/val) Mean, 64.6 Mean, 61.7 No significant association gao et al, 2003(9) Chinese 48 112 CYP1A1 (Ile/val) … … A/g associated with PC risk Murata et al, 1998(28) Japanese 115 204 CYP1A1 (MspI) CYP1A1 (Ile/Val) Mean, 73 Mean, 71 Ile/Val and Val/Val associated with PC risk guan et al, 2005(30) Chinese 83 115 gene Chip Technique … … No significant association Vijayalakshmi et al, 2005(29) South Indian 100 100 CYP1A1 (MspI) CYP1A1 (Ile/Val) … … T/C associated with increased risk, A/g associated with decreased risk of PC Table 4. Results of CYP1A1 Polymorphisms in Some Additional Studies* *SNP indicates single nucleotide polymorphism; CYP1A1, cytochrome P450, family 1, subfamily A, polypeptide 1; and PC, prostate cancer. Figure 1. Odds ratio and 95% confidence interval of the distribution of CYP1A1 MspI polymorphism (TC genotype). Figure 2. Odds ratio and 95% confidence interval of the distribution of CYP1A1 Ile/Val polymorphism (Ag genotype). CYP1A1 and Prostate Cancer—Shaik et al Urology Journal Vol 6 No 2 Spring 2009 83 studies, Funnel plots were constructed for both T/C and A/G polymorphisms (Figures 3 and 4). DISCUSSION CYP1A1 is likely to play an important role in the etiology of prostate cancer through its function in activating environmental procarcinogens and catalyzing the oxidative metabolites of estrogens. To test the hypothesis that genetic polymorphisms in the CYP1A1 gene may be associated with the risk of prostate cancer, studies have been performed in various populations. In Chinese men,(15) 3801T/C and 2455A/G were each individually associated with the risk of prostate cancer. Beer and colleagues(18) performed genotyping of CYP1A1 (Ile/Val) gene in 117 patients with prostate cancer and 183 population- based controls. Their cohort failed to identify a relationship between the above polymorphisms and prostate cancer. Atkas and coworkers(19) studied the association of CYP1A1 with prostate cancer in 100 patients and 107 controls of Turkish origin. No statistical differences were observed in the distribution of the CYP1A1 Ile/Val genotype between the two groups (OR, 1.076; 95% CI, 0.605 to 1.913). However, the patients with CYP1A1 Val/Val revealed a 2.8-fold higher risk of having prostate cancer than those with the wild- type Ile/Ile (OR, 2.846; 95% CI, 1.004 to 8.064). Vijayalakshmi and associates(29) investigated the Effect Size and 95% Confidence Interval Test of Null (2-Tail) heterogeneity Model Number of Studies Point Estimate Lower Limit Upper Limit Z P Q df(Q) P I-Squared Fixed 8 1.354 1.150 1.594 3.645 < .001 9.799 7 .20 28.561 Random 8 1.350 1.110 1.641 3.007 .003 … … … … Table 5. Heterogeneity Between Study Populations Assessed for CYP1A1 MspI Polymorphism (TC genotype)* *Ellipses indicate not applicable. Effect Size and 95% Confidence Interval Test of Null (2-Tail) heterogeneity Model Number of Studies Point Estimate Lower Limit Upper Limit Z P Q df(Q) P I-Squared Fixed 7 1.117 0.922 1.354 1.130 .26 7.968 6 .24 24.702 Random 7 1.085 0.863 1.364 0.696 .49 … … … … Table 6. Heterogeneity Between Study Populations Assessed for CYP1A1 Ile/Val Polymorphism (Ag genotype) Figure 3. Funnel plot to estimate the amount of publication bias in studies on CYP1A1 MspI polymorphism (TC genotype). Figure 4. Funnel plot to estimate the amount of publication bias in studies on CYP1A1 Ile/Val polymorphism (Ag genotype). CYP1A1 and Prostate Cancer—Shaik et al 84 Urology Journal Vol 6 No 2 Spring 2009 association between two SNP’s in South Indian population. Individuals with w1/m1 genotype at 3’UTR of CYP1A1 were at a higher risk of prostate cancer (OR, 4.64; 95% CI, 1.51 to 14.86; P < .01), while the CYP1A1 Ile/Val genotype (w2/m2) on exon 7 was found to be associated with a decreased risk of the cancer (OR, 0.17; 95% CI, 0.02 to 0.89; P = .03). Different grades of tumors did not have a significant association with the variant genotypes. The role of CYP1A1, cigarette smoking, and age was analyzed by Mittal and Srivastava(20) in 130 patients with prostate cancer and 140 controls using polymerase chain reaction assay and binary logistic regression model. The T/C polymorphism of CYP1A1 revealed a significant association with smoking for prostate cancer risk. Li and colleagues(21) analyzed CYP1A1 with respect to genetic susceptibility to prostate cancer in Chinese men. They genotyped 208 patients and 230 age-matched controls and analyzed the results according to age at diagnosis, prostate-specific antigen levels, and cancer stage and grade (Gleason score). No significant differences in the frequency distributions of CYP1A1 polymorphisms were observed between the patients and the controls. In another study, CYP1A1 was analyzed in a case-control fashion, but the data was not statistically significant after appropriate corrections for multiple comparisons.(16) Yang and colleagues(22) investigated the association of cytochrome P450 1A1, smoking, alcohol drinking, and the risk of prostate cancer in a Han population in Southern China (225 patients and 250 age-matched controls). The CYP1A1 Val/ Val genotype significantly increased the risk of prostate cancer (OR, 2.26; 95% CI, 1.09 to 4.68). Heavy smoking history (OR, 1.61; 95% CI, 1.04 to 2.50) significantly increased the susceptibility of prostate cancer. Nock and coworkers(17) investigated the relationship between cigarette smoking and CYP1A1 Ile/Val polymorphism using a family- based case-control design (439 patients with prostate cancer and 479 controls); however, the results were not statistically significant. In another study, 83 patients and 115 age-matched healthy controls were genotyped for genetic polymorphisms of CYP1A1 by the genechip technique. There were no significant differences in the frequency of CYP1A1 polymorphisms between the patients and the healthy controls.(30) Silig and colleagues(23) studied on CYP1A1- MspI in 321 Turkish individuals (152 patients with prostate cancer and 169 age-matched controls). No association was observed between CYP1A1 polymorphism and prostate cancer or smoking history. Associations between genetic polymorphisms of CYP1A1 and prostate cancer were analyzed by Murata and associates(32) in a case-control study of 315 individuals. The frequency of Val/Val genotypes for CYP1A1 was 11.3% in patients with cander compared with 5.5% in controls. This polymorphism, thus, was associated with a significantly increased risk of prostate cancer (OR, 2.4; 95% CI; 1.01 to 5.57). The study also confirmed that the CYP1A1 polymorphism in combination with glutathione S-transferase M1 (GSTM1) gene polymorphism may be associated with prostate cancer susceptibility in the Japanese population. Gao and colleagues(9) studied the possible relationship between CYP1A1 genetic polymorphisms and the susceptibility of prostate cancer in 48 patients and 112 healthy individuals. Among patients and their matched controls, the frequencies of alleles and genotypes were significantly different with Ile/Val gene polymorphisms (P < .05); the allele G and GG genotypes were significantly more frequent than those in the controls with an (OR, 1.59 and OR, 3.06; respectively). But, no significant differences of the frequencies of the MspI alleles and genotypes were found between the patients with prostate cancer and the controls. The association between genetic polymorphisms of CYP1A1 and familial prostate cancer risk was examined in a case-control study of 185 individuals by Suzuki and associates.(24) The presence of any mutated alleles significantly increased cancer risk in comparison with wild- type genotypes by combination analysis (OR, 2.38; 95% CI, 1.72 to 3.29; P = .007). Acevedo and colleagues(25) studied on the associations between CYP1A1 Msp1 and prostate cancer in a case-control study. Their findings suggest that the CYP1A1 and Prostate Cancer—Shaik et al Urology Journal Vol 6 No 2 Spring 2009 85 Chilean carrying single or combined GSTM1 and CYP1A1 polymorphisms were more susceptible to prostate cancer. Caceres and colleagues(26) suggested that the interaction between genetic polymorphisms in GST (T1;M1) and CYP1A1 M1 would play a significant role as a modifying factor on prostate cancer risk in Chilean people. Figer and coworkers(27) showed in 224 patients that CYP1A1 gene polymorphisms did not show a significant association with prostate cancer. Finally, Murata and coworkers(28) analyzed genetic polymorphisms of the xenobiotic-metabolizing enzymes, CYP1A1, and GSTM1 in 115 patients with cancer and 204 controls. The CYP1A1 Val/ Val genotype significantly increased the risk of prostate cancer (OR, 2.6; 95% CI, 1.11 to 6.25) and the Ile/Val genotype showed a similar tendency (OR, 1.4; 95% CI, 0.86 to 2.29). The combination of the CYP1A1 Val allele and GSTM1 (0/0) genotype was associated with a higher risk (OR, 2.3; 95% CI, 1.18 to 4.48) than the CYP1A1 Val allele alone. CONCLUSION This meta-analysis included data from 14 case- control comparisons with approximately 6000 genotyped patients with prostate cancer and controls. The overall data demonstrated that the CYP1A1 G/A polymorphism is unlikely to be a major risk factor of prostate cancer on a wide population basis. However, although individual studies have shown conflicting results, the T/C polymorphism may considerably influence the risk of this cancer. The CYP1A1 polymorphism, therefore, may be an important population-wide risk factor of prostate cancer with respect to the T/C polymorphism. This meta-analysis could not address conclusively familial prostate cancer because hereditary forms of this cancer with many members affected in a family are not very common. Future studies are being planned to explore whether the CYP1A1 polymorphism may have any effects on the risk of prostate cancer specifically in this setting. Control groups of the different studies were not well characterized as to the extent of inclusion of subjects with benign prostatic hyperplasia, which may again affect the results. Studies are also planned to determine the influence of T/C polymorphism with the risk of prostate cancer on a wider population basis. ACKNOwLEDgMENT The authors would like to thank Indo-American Cancer Hospital and Research Centre and Bhagwan Mahavir Medical Research Centre for facilities provided. They would also like to acknowledge the colleagues for providing expert advice in shortlisting the studies and for evaluating the studies for meta-analyses. CONFLICT OF INTEREST None declared. REFERENCES 1. Kawajiri K. Cyp1a1. IARC Sci Publ. 1999;(148):159- 72. 2. Haas gP, Sakr wA. Epidemiology of prostate cancer. CA Cancer J Clin. 1997;47:273-87. 3. 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