# bib

id: cord-000315-rfwzj1at
author: Riaz Shah, Shahida Amjad
title: Hepatitis G Virus associated aplastic anemia: A recent case from Pakistan
date: 2011-01-21
extension: .txt
mime: text/plain
words: 1530
sentence: 79
flesch: 43
cache: cache/cord-000315-rfwzj1at.txt
txt: txt/cord-000315-rfwzj1at.txt
summary:
  The incidence of transfusion-associated hepatitis G virus infection and its relation to liver disease Hepatitis GB virus C genome in the serum of aplastic anaemia patients receiving frequent blood transfusions Prevalence of GBV-C/HGV, a novel 'hepatitis' virus, in patients with aplastic anaemia Hepatitis G virus: molecular organization, methods of detection, prevalence, and disease association Hepatitis G Virus associated aplastic anemia: Adil et al has reported, severe aplastic anaemia (SAA) 51.4%, very severe (VSAA) in 16.7% of 144 patients of aplastic anemia cases


id: cord-003855-so8xl199
author: Ebert, Gregor
date: 2019-09-02
extension: .txt
mime: text/plain
words: 1901
sentence: 67
flesch: 31
cache: cache/cord-003855-so8xl199.txt
txt: txt/cord-003855-so8xl199.txt
title:
  Virology Downunder, a meeting commentary from the 2019 Lorne Infection and Immunity Conference, Australia

summary:
  The 'Infection and Inflammation' session of the meeting was opened with a well-received presentation by Linfa Wang (Duke-NUS Medical School, Singapore) entitled 'Holy immune balance, batman', about the highly adapted immune response of bats to viral infections. During their evolutionary adaption to effective flight, bats not only developed elevated levels of basal alertness reflected in increased metabolic heart rate and body temperature, they also seem to have evolved a highly adapted immune defense against viral infections [3] .


id: cord-286842-04cuk2cn
author: Plyusnina, Angelina
date: 2005-02-22
extension: .txt
mime: text/plain
words: 2148
sentence: 80
flesch: 43
cache: cache/cord-286842-04cuk2cn.txt
txt: txt/cord-286842-04cuk2cn.txt
title:
  Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture

summary:
  Recombination in RNA viruses serves two main purposes: (i) it generates and spreads advantageous genetic combinations; and (ii) it counters the deleterious effect of mutations that, due to the low fidelity of viral RNA polymerases and lack of proofreading, occur with high frequency [1] . Taking into account that the in situ formed recombinant S RNA disappeared from the mixture after four passages [11] , one should conclude that the lower competitiveness of the recombinant virus seen earlier did not result from its immature status.


id: cord-000640-t0y0b0gb
author: Sumibcay, Laarni
date: 2012-01-26
extension: .txt
mime: text/plain
words: 2222
sentence: 82
flesch: 38
cache: cache/cord-000640-t0y0b0gb.txt
txt: txt/cord-000640-t0y0b0gb.txt
title:
  Divergent lineage of a novel hantavirus in the banana pipistrelle (Neoromicia nanus) in Côte d'Ivoire

summary:
  To investigate this possibility, archival tissues from 213 insectivorous bats (order Chiroptera) were analyzed for hantavirus RNA by RT-PCR. Following numerous failed attempts, hantavirus RNA was detected in ethanol-fixed liver tissue from two banana pipistrelles (Neoromicia nanus), captured near Mouyassué village in Côte d'Ivoire, West Africa, in June 2011.


id: cord-048368-wm4c7rk6
author: Evseenko, Vasily A
title: Experimental infection of H5N1 HPAI in BALB/c mice
date: 2007-07-27
extension: .txt
mime: text/plain
words: 2888
sentence: 160
flesch: 51
cache: cache/cord-048368-wm4c7rk6.txt
txt: txt/cord-048368-wm4c7rk6.txt
summary:
  It was shown that H5N1 HPAI viruses could have different pathogenicity for mice [7] . The analysis of non-structural protein 1 (NS1) which also could be contributed for high virulence of H5N1 viruses revealed deletion of 5 amino acids similar to those in H5N1 viruses of genotype Z which could be contributed to increased expression of TNF-α and IP-10 protein in primary human macrophages [18] .


id: cord-291961-usl8z6ep
author: Zheng, Wen-zhi
date: 2015-10-13
extension: .txt
mime: text/plain
words: 2937
sentence: 131
flesch: 50
cache: cache/cord-291961-usl8z6ep.txt
txt: txt/cord-291961-usl8z6ep.txt
title:
  Human polyomavirus type six in respiratory samples from hospitalized children with respiratory tract infections in Beijing, China

summary:
  The full-length HPyV6 sequence was aligned with the sequences of other HPyVs and other HPyV6 strains available in GenBank with DNAStar software. All 15 HPyV6-positive patients were coinfected with other respiratory viruses, of which influenza virus A (IFVA) (8/15, 53.3 %) and respiratory syncytial virus (7/15, 46.7 %) were most common.


id: cord-290798-5ca2e6wm
author: Malhotra, Bharti
date: 2016-06-06
extension: .txt
mime: text/plain
words: 3019
sentence: 117
flesch: 49
cache: cache/cord-290798-5ca2e6wm.txt
txt: txt/cord-290798-5ca2e6wm.txt
title:
  Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses

summary:
  RVP fast assay and the idaho technology filmarray RP assay for detection of respiratory viruses in pediatric patients at a cancer hospital Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in hospitalized children with acute respiratory infections in Beijing, China Detection of all known parechoviruses by real-time PCR? Clinical evaluation of multiplex real-time pcr panels for rapid detection of respiratory viral infections Molecular assays for quantitative and qualitative detection of influenza virus and oseltamivir resistance mutations Validation -FTD Respiratory pathogens 21. Luxembourg: Fast Track Diagnostics Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood Viral aetiology of acute lower respiratory tract illness in hospitalized paediatric patients of a tertiary hospital: One year prospective study Real-Time PCR Assays for Detection of Bocavirus in Human Specimens During the summer 2009 outbreak of swine flu in Scotland what respiratory pathogens were diagnosed as H1N1/2009? Comparison of three multiplex PCR assays for the detection of respiratory viral infections: Evaluation of xTAG


id: cord-274375-a11ztdpg
author: Zhang, Yiqiang
title: Analysis of synonymous codon usage in Hepatitis A virus
date: 2011-04-16
extension: .txt
mime: text/plain
words: 3216
sentence: 134
flesch: 47
cache: cache/cord-274375-a11ztdpg.txt
txt: txt/cord-274375-a11ztdpg.txt
summary:
  The extent of codon usage bias in human RNA viruses and its evolutionary origin Genetic variability and molecular evolution of Hepatitis A virus Genome variability and capsid structural constraints of hepatitis A virus Codon usage and replicative strategies of hepatitis A virus Codon Usage and Genome Composition Compositional Constraints and Genome Evolution Synonymous codon usage in adenoviruses: Influence of mutation, selection and protein hydropathy Analysis of synonymous codon usage in 11 Human Bocavirus isolates Mutation rates among RNA viruses Analysis of sequential hepatitis A virus strains reveals coexistence of distinct viral subpopulations Genetic relatedness of hepatitis A virus strains recovered from different geographical regions Molecular epidemiology of human hepatitis A virus defined by an antigen-capture/polymerase chain reaction method Epidemiologic patterns of wild-type hepatitis A virus determined by genetic variation The 'effective number of codons' used in a gene An evaluation of measures of synonymous codon usage bias Multivariate analysis NewYork Analysis of synonymous codon usage in Hepatitis A virus This work was supported in parts by grants from National Key Technologies Authors' contributions YQZ conceived of the study, downloaded these sequences, calculated the data, analyzed the results and drafted the manuscript; YSL conceived of the study, supervised the research, analyzed the results and helped draft the manuscript; JHZ calculated and visualized the data; WQL, HTC, YW, LNM and YZD assisted with data analysis; JZ supervised the research and helped draft the manuscript. key: cord-274375-a11ztdpg authors: Zhang, Yiqiang; Liu, Yongsheng; Liu, Wenqian; Zhou, Jianhua; Chen, Haotai; Wang, Yin; Ma, Lina; Ding, Yaozhong; Zhang, Jie title: Analysis of synonymous codon usage in Hepatitis A virus date: 2011-04-16 journal: Virol J DOI: 10.1186/1743-422x-8-174 sha: doc_id: 274375 cord_uid: a11ztdpg BACKGROUND:


id: cord-000389-9vnthmfn
author: Guo, Xichao
date: 2011-05-10
extension: .txt
mime: text/plain
words: 3282
sentence: 134
flesch: 51
cache: cache/cord-000389-9vnthmfn.txt
txt: txt/cord-000389-9vnthmfn.txt
title:
  Dynamic variations in the peripheral blood lymphocyte subgroups of patients with 2009 pandemic H1N1 swine-origin influenza A virus infection

summary:
  The NK cell counts of patients with moderate H1N1 were similar to that of patients with severe H1N1, which demonstrated a gradual decline, whereas the frequency of CD19 + B and CD8 + T cells showed no significant changes. Therefore, monitoring the variation of cellular immune functions in H1N1 patients have important clinical significance.


id: cord-292581-6ipzvryb
author: Alagarasu, Kalichamy
title: Elevated levels of vitamin D and deficiency of mannose binding lectin in dengue hemorrhagic fever
date: 2012-05-04
extension: .txt
mime: text/plain
words: 3747
sentence: 172
flesch: 47
cache: cache/cord-292581-6ipzvryb.txt
txt: txt/cord-292581-6ipzvryb.txt
summary:
  Although MBL levels were not different DF and DHF cases based on immune status, the percentage of primary DHF cases (50%) having MBL levels lower than 500 ng/ml were less compared to primary DF cases (P = 0.038). Vitamin D concentrations were not significantly different between primary DF and primary DHF cases (P > 0.050).


id: cord-271948-iq29xqrn
author: Obeng, Billal Musah
date: 2020-07-24
extension: .txt
mime: text/plain
words: 3583
sentence: 159
flesch: 51
cache: cache/cord-271948-iq29xqrn.txt
txt: txt/cord-271948-iq29xqrn.txt
title:
  Transmitted drug resistance mutations and subtype diversity amongst HIV-1 sero-positive voluntary blood donors in Accra, Ghana

summary:
  Sequences were submitted to the Stanford University HIV Drug Resistance Database (https:// hivdb.stanford.edu/hivdb/by-sequences/) to assign subtypes detect HIV drug resistance mutations and. key: cord-271948-iq29xqrn authors: Obeng, Billal Musah; Bonney, Evelyn Yayra; Asamoah-Akuoko, Lucy; Nii-Trebi, Nicholas Israel; Mawuli, Gifty; Abana, Christopher Zaab-Yen; Sagoe, Kwamena William Coleman title: Transmitted drug resistance mutations and subtype diversity amongst HIV-1 sero-positive voluntary blood donors in Accra, Ghana date: 2020-07-24 journal: Virol J DOI: 10.1186/s12985-020-01386-y sha: doc_id: 271948 cord_uid: iq29xqrn BACKGROUND: Detection of HIV-1 transmitted drug resistance (TDR) and subtype diversity (SD) are public health strategies to assess current HIV-1 regimen and ensure effective therapeutic outcomes of antiretroviral therapy (ART) among HIV-1 patients.


id: cord-310372-qc6941pm
author: Ji, Jun
date: 2011-04-22
extension: .txt
mime: text/plain
words: 3576
sentence: 134
flesch: 42
cache: cache/cord-310372-qc6941pm.txt
txt: txt/cord-310372-qc6941pm.txt
title:
  Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009

summary:
  virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or hemagglutination-inhibiting antibody, or induce chicken tracheal protection Relationship between variation in the S1 spike protein of infectious bronchitis virus and the extent of crossprotection Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Antigenic and S-1 genomic characterization of the Delaware variant serotype of infectious bronchitis virus Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes Analysis of the serotypespecific epitopes of avian infectious bronchitis virus strains Ark99 and Mass41 Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses Different genotypes of nephropathogenic infectious bronchitis viruses co-circulating in chicken population in China Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in South China during Infectious bronchitis virus S1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization Isolation and identification of four infectious bronchitis virus strains in China and analyses of their S1 glycoprotein gene Current status of infectious bronchitis virus infections in chickens and prospects for control by vaccines Maternal antibody to infectious bronichitis virus: its role in protection against infection and development of active immunity to vaccine A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and nonvaccinated flocks in China Genotypic and phenotypic characterization of the California 99 (Cal99) variant of infectious bronchitis virus Co-circulation of four types of infectious bronchitis virus (793/B, 624/I, B1648 and Massachusetts) Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs A survey of the presence of a new infectious bronchitis virus designated 4/91 (793B) A nomenclature for avian coronavirus isolates and the question of species status Infectious bronchitis Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus The experimental infection of chickens with mixtures of infectious bronchitis virus and Escherichia coli Significance of interactions between Escherichia coli and respiratory pathogens in layer hen flocks suffering from colibacillosisassociated mortality Cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus The molecular biology of coronaviruses Coronavirus IBV: removal of spike glycopolypeptide S1 by urea abolishes infectivity and haemagglutination but not attachment to cells Location of the amino-acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Coronavirus IBV:


id: cord-289081-9v04gzhf
author: Aponte, Fernando E
date: 2015-02-26
extension: .txt
mime: text/plain
words: 3753
sentence: 148
flesch: 44
cache: cache/cord-289081-9v04gzhf.txt
txt: txt/cord-289081-9v04gzhf.txt
title:
  Rhinovirus is an important pathogen in upper and lower respiratory tract infections in Mexican children

summary:
  The incidence of RV infection was highest in September and November 2011 as well as in April 2012, when it accounted for 24.4%, 27.1% and 30.3%, respectively, of the total samples collected ( Figure 1 ). This was true for single and mixed RV infections.


id: cord-283333-u3r1usfs
author: Yao, Li-hong
date: 2019-06-13
extension: .txt
mime: text/plain
words: 3727
sentence: 153
flesch: 45
cache: cache/cord-283333-u3r1usfs.txt
txt: txt/cord-283333-u3r1usfs.txt
title:
  Human adenovirus among hospitalized children with respiratory tract infections in Beijing, China, 2017–2018

summary:
  [33] , and it was the main type of respiratory HAdV infections from 1981 to 2002. Therefore, the aim of this study was to evaluate the epidemiological, clinical, and molecular characteristics of HAdV infections occurring among children with RTIs in a Chinese tertiary hospital from April 2017 to March 2018.


id: cord-303494-tofch4j7
author: Bai, Juan
title: Identification of VP1 peptides diagnostic of encephalomyocarditis virus from swine
date: 2014-12-30
extension: .txt
mime: text/plain
words: 3864
sentence: 150
flesch: 51
cache: cache/cord-303494-tofch4j7.txt
txt: txt/cord-303494-tofch4j7.txt
summary:
  Immunization of mice with recombinant VP1 protein (plasmids or recombinant adenoviruses expressing small hairpin RNAs targeted to VP1 protein genes of EMCV), which could provide protective efficacy against EMCV [26, 27] . EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective immunity.


id: cord-263976-b9shffb3
author: Shaukat, Shahzad
date: 2014-08-12
extension: .txt
mime: text/plain
words: 3694
sentence: 149
flesch: 40
cache: cache/cord-263976-b9shffb3.txt
txt: txt/cord-263976-b9shffb3.txt
title:
  Identification and characterization of unrecognized viruses in stool samples of non-polio acute flaccid paralysis children by simplified VIDISCA

summary:
  Emerg Infect Dis Identification of human parechovirus genotype, HPeV-12, in a paralytic child with diarrhea Human parechovirus 3 and neonatal infections Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis Detection and genetic differentiation of human astroviruses: phylogenetic grouping varies by coding region Molecular epidemiology of human astrovirus diarrhea among children from a periurban community of Mexico City Evidence of a recombinant wild-type human astrovirus strain from a Kenyan child with gastroenteritis Emergence of unique variants and inter-genotype recombinants of human astroviruses infecting infants, children and adults in Kolkata Use of nucleotide composition analysis to infer hosts for three novel picorna-like viruses A new arenavirus in a cluster of fatal transplantassociated diseases Metagenomic analysis of human diarrhea: viral detection and discovery Applications of next-generation sequencing technologies to diagnostic virology Molecular identification and characterization of a new type of Bovine Enterovirus A newly identified bocavirus species in human stool A highly prevalent and genetically diversified Picornaviridae genus in South Asian children Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by realtime reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues Rapid and simple method for purification of nucleic acids Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses Human parechovirus infections in Dutch children and the association between serotype and disease severity MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0 A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences Identification and characterization of unrecognized viruses in stool samples of non-polio acute flaccid paralysis children by simplified VIDISCA Here, we show that in the majority of virus cultures simplified-VIDISCA can quickly reveal the infecting agent.


id: cord-300908-i80tuhqk
author: Yu, Fuxun
date: 2015-08-04
extension: .txt
mime: text/plain
words: 4204
sentence: 164
flesch: 47
cache: cache/cord-300908-i80tuhqk.txt
txt: txt/cord-300908-i80tuhqk.txt
title:
  Application of recombinant severe fever with thrombocytopenia syndrome virus nucleocapsid protein for the detection of SFTSV-specific human IgG and IgM antibodies by indirect ELISA

summary:
  The expression construct encompassing amino acid (aa) 1-246, the full length of SFTSV N protein with a vector derived His-tag (histidine hexmer) at the N-terminus, was obtained. Recombinant N protein of Rift Valley Fever (RVF) virus, another member of the Phlebovirus genus, was reported to be used in a detection system for the laboratory diagnosis of RFV infection in humans and animals


id: cord-254384-mwzz1db5
author: Lu, Guilan
date: 2011-02-10
extension: .txt
mime: text/plain
words: 3984
sentence: 172
flesch: 50
cache: cache/cord-254384-mwzz1db5.txt
txt: txt/cord-254384-mwzz1db5.txt
title:
  Large-scale seroprevalence analysis of human metapneumovirus and human respiratory syncytial virus infections in Beijing, China

summary:
  To assess the seroprevalence of hMPV infection in China, we used hMPV N protein as an antigen to test serum samples for the presence of anti-hMPV IgG antibody in children and adults free of acute respiratory illness in Beijing, China. [19] , it has been widely applied in the immunoassay of hMPV infection and in the investigation of seroprevalence of hMPV infections [17, 18] .


id: cord-284630-l9ghggu7
author: Hoang, Minh
title: Molecular epidemiology of canine parvovirus type 2 in Vietnam from November 2016 to February 2018
date: 2019-04-27
extension: .txt
mime: text/plain
words: 4133
sentence: 206
flesch: 55
cache: cache/cord-284630-l9ghggu7.txt
txt: txt/cord-284630-l9ghggu7.txt
summary:
  Canine parvovirus: a review of epidemiological and diagnostic aspects, with emphasis on type 2c Nucleotide sequence and genome organization of canine parvovirus The emergence of parvoviruses of carnivores Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis Natural variation of canine parvovirus Evidence for evolution of canine parvovirus type 2 in Italy A canine parvovirus mutant is spreading in Italy Characterisation of the canine parvovirus type 2 variants using minor groove binder probe technology Occurrence of canine parvovirus type 2c in the United States First detection of canine parvovirus type 2c in South America Evolution of canine parvovirus in Argentina between years 2003 and 2010: CPV2c has become the predominant variant affecting the domestic dog population Genotyping of canine parvovirus in western Mexico Molecular epidemiology of canine parvovirus in Morocco Molecular characterization of canine parvovirus-2 variants circulating in Tunisia A novel antigenic variant of canine parvovirus from a Vietnamese dog Identification of a novel canine parvovirus type 2c in Taiwan Co-circulation of the rare CPV-2c with unique Gln370Arg substitution, new CPV-2b with unique Thr440Ala substitution, and new CPV-2a with high prevalence and variation in Heilongjiang Province Typing of canine parvovirus strains circulating in North-East China Typing of canine parvovirus strains circulating in North-East China Continuing evolution of canine parvovirus in China: isolation of novel variants with an Ala5Gly mutation in the VP2 protein Phylodynamic and genetic diversity of canine parvovirus type 2c in Taiwan Molecular characterization of canine parvovirus in Vientiane Emergence of canine parvovirus type 2c in domestic dogs and cats from Thailand Development of SYBR green-based real-time PCR for the detection of canine, feline and porcine parvoviruses A SimpleProbe® real-time PCR assay for differentiating the canine parvovirus type 2 genotypereal-time PCR assay for differentiating the canine parvovirus type 2 genotype MEGA6: molecular evolutionary genetics analysis version 6.0 Canine parvovirus in New Zealand: epidemiological features and diagnostic methods Risk factors associated with parvovirus enteritis in dogs: 283 cases (1982-1991) Canine parvovirus in a commercial kennel: epidemiologic and pathologic findings Aspects of the diagnosis, pathogenesis and epidemiology of canine parvovirus Parvovirus enteritis in dogs based on autopsy statistics 1978-1985 Clinical and epidemiological aspects of canine parvovirus (CPV) enteritis in the State of Molecular epidemiology of canine parvovirus in Southern India Incidence of canine parvovirus in diarrhoeic dogs by polymerase chain reaction Frequency of CPV infection in vaccinated puppies that attended puppy socialization classes A modified live canine parvovirus vaccine.


id: cord-273179-bpnak9ov
author: Ma, Fen-lian
date: 2017-08-14
extension: .txt
mime: text/plain
words: 4130
sentence: 175
flesch: 55
cache: cache/cord-273179-bpnak9ov.txt
txt: txt/cord-273179-bpnak9ov.txt
title:
  Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real-time TaqMan-based PCR

summary:
  Sixteen specimens were positive for MWPyV, including 13 (1.47%) respiratory samples and three (1.7%) fecal samples. These findings are supported by other studies that frequently detected MWPyV in respiratory samples from acutely ill patients and babies with upper respiratory symptoms [13, 17] .


id: cord-277547-2vim1wno
author: Zandi, Keivan
title: Antiviral activity of four types of bioflavonoid against dengue virus type-2
date: 2011-12-28
extension: .txt
mime: text/plain
words: 4387
sentence: 193
flesch: 47
cache: cache/cord-277547-2vim1wno.txt
txt: txt/cord-277547-2vim1wno.txt
summary:
  Anti-dengue activity of these compounds was determined at different stages of DENV-2 infection and replication cycle. Although there was no significant direct virucidal activity against DENV-2 by quercetin, continuous treatment of cells from 5 h before virus infection up to 4 days post-infection exhibited anti-dengue activity with IC 50 = 28.9 μg mL -1


id: cord-273711-bxijla09
author: Zhao, Zhixun
date: 2012-02-17
extension: .txt
mime: text/plain
words: 4185
sentence: 175
flesch: 46
cache: cache/cord-273711-bxijla09.txt
txt: txt/cord-273711-bxijla09.txt
title:
  RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells

summary:
  Transfection of the siRNA expression cassettes into BHK-21 cells BHK-21 cells were seeded in six-well plates and cultured at 37°C and 5% CO2 overnight. To determine transfection efficiency, we monitored GFP fluorescence intensity of transfected cells using fluorescent microscope analysis.


id: cord-269277-x5c5ogo7
author: Li, Jingjing
title: Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-activation by NS1 protein
date: 2013-10-27
extension: .txt
mime: text/plain
words: 4160
sentence: 172
flesch: 51
cache: cache/cord-269277-x5c5ogo7.txt
txt: txt/cord-269277-x5c5ogo7.txt
summary:
  Dual-luciferase reporter vectors were employed in order to evaluate critical promoter elements and the effect of NS1 protein on promoter activity. Mutations introduced in the CAAT box of the HBoV1 promoter reduced promoter activity by 34%, whereas nucleotide substitutions in the TATA box had no effect on promoter activity.


id: cord-254713-ghcwfcx2
author: Razanajatovo, Norosoa H
title: Detection of new genetic variants of Betacoronaviruses in Endemic Frugivorous Bats of Madagascar
date: 2015-03-12
extension: .txt
mime: text/plain
words: 4165
sentence: 163
flesch: 44
cache: cache/cord-254713-ghcwfcx2.txt
txt: txt/cord-254713-ghcwfcx2.txt
summary:
  Some authors have reported the detection of bat CoVs from mainland Africa Thus, further molecular epidemiology studies would be required to fully understand the dispersal potential of CoVs amongst Malagasy bats species.


id: cord-283689-dzin12qb
author: Zhang, Wei
title: Involvement of PRRSV NSP3 and NSP5 in the autophagy process
date: 2019-01-28
extension: .txt
mime: text/plain
words: 5154
sentence: 253
flesch: 47
cache: cache/cord-283689-dzin12qb.txt
txt: txt/cord-283689-dzin12qb.txt
summary:
  We identified some of the effects of these two transmembrane proteins on cell autophagy to determine whether the NSPs affected cytoplasmic membrane fusion. Although NSP3 and NSP5 are ER transmembrane proteins, these proteins did not activate the ER stress signaling pathways.


id: cord-303313-b8lesr2g
author: Clem, Amy L
title: Virus detection and identification using random multiplex (RT)-PCR with 3'-locked random primers
date: 2007-06-28
extension: .txt
mime: text/plain
words: 5497
sentence: 406
flesch: 67
cache: cache/cord-303313-b8lesr2g.txt
txt: txt/cord-303313-b8lesr2g.txt
summary:
  Expression of mouse interleukin-4 by a recombinant ectromelia virus suppresses cytolytic lymphocyte responses and overcomes genetic resistance to mousepox Detection of smallpox virus DNA by LightCycler PCR Fatal yellow fever in a traveler returning from Amazonas, Brazil Sequence-specific identification of 18 pathogenic microorganisms using microarray technology Estimation of effective population size of HIV-1 within a host: a pseudomaximum-likelihood approach Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers RNA fingerprinting using arbitrarily primed PCR identifies differentially regulated RNAs in mink lung (Mv1Lu) cells growth arrested by transforming growth factor beta 1 Osterhaus AD: Unfortunately, high mutation rates may lead to extensive changes in viral nucleic acid sequences making dedicated PCR primer use problematic.


id: cord-048466-fj9l8che
author: Bragstad, Karoline
title: The evolution of human influenza A viruses from 1999 to 2006: A complete genome study
date: 2008-03-07
extension: .txt
mime: text/plain
words: 5362
sentence: 224
flesch: 46
cache: cache/cord-048466-fj9l8che.txt
txt: txt/cord-048466-fj9l8che.txt
summary:
  Influenza: the Mother of All Pandemics Recent changes among human influenza viruses Mild to moderate influenza activity in Europe and the detection of novel A(H1N2) and B viruses during the winter of 2001-02 Influenza AH1N2 viruses, United Kingdom, 2001-02 influenza season Molecular Evolution of Human Influenza A/H3N2 Virus in Asia adn Europe from An Influenza A (H3) Reassortant Was Epidemic in Australia and New Zealand in 2003 Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution The value of a database in surveillance and vaccine selection Egg fluids and cells of the chorioallantoic membrane of embryonated chicken eggs can select different variants of influenza A (H3N2) viruses Natural Selection on the Influenza Virus Genome Human memory CTL response specific for influenza A virus is broad and multispecific Influenza A Virus Neuraminidase: Regions of the Protein Potentially Involved in Virus-Host Interactions CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening HLA-A1 and HLA-A3 T cell epitopes derived from influenza virus proteins predicted from peptide binding motifs Different length peptides bind to HLA-Aw68 similarly at their ends but bulge out in the middle Assessment of the extent of variation in influenza A virus cytotoxic T-lymphocyte epitopes by using virus-specific CD8+ T-cell clones The HLA-B14 peptide binding site can accommodate peptides with different combinations of anchor residues The peptide binding motif of the disease associated HLA-DQ (alpha 1* 0501, beta 1* 0201) molecule HLA-B*0702 transgenic, H-2KbDb doubleknockout mice: phenotypical and functional characterization in response to influenza virus Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern Molecular basis of resistance of influenza A viruses to amantadine Human CD8+ and CD4+ T lymphocyte memory to influenza A viruses of swine and avian species Identification of the peptide binding motif for HLA-B44, one of the most common HLA-B alleles in the Caucasian population Emergence of Influenza A H1N2 Reassortant Viruses in the Human Population during Intercontinental circulation of human influenza A(H1N2) reassortant viruses during the 2001-2002 influenza season Phylogenetic analysis of the entire genome of influenza A (H3N2) viruses from Japan: evidence for genetic reassortment of the six internal genes Wholegenome analysis of human influenza Until the recent release of over 1,800 complete influenza A genome sequences from the Influenza Genome Sequencing Project managed by US National Institute of Allergy and Infectious Diseases [7, 8] The relative prevalence of influenza virus varies from season to season.


id: cord-031316-yvid6qps
author: Bisimwa, Patrick N.
date: 2020-09-03
extension: .txt
mime: text/plain
words: 5414
sentence: 225
flesch: 50
cache: cache/cord-031316-yvid6qps.txt
txt: txt/cord-031316-yvid6qps.txt
title:
  First detection of African swine fever (ASF) virus genotype X and serogroup 7 in symptomatic pigs in the Democratic Republic of Congo

summary:
  Phylogeographic analysis of African swine fever virus Evolution in Europe of African swine fever genotype II viruses from highly to moderately virulent Molecular characterization of African swine fever virus Pig empire under infectious threat: risk of African swine fever introduction into the People's Republic of China EMPRES Watch: African swine fever in the Caucasus Epidemiology of African swine fever in Poland since the detection of the first case African swine fever virus replication and genomics Identification of a new genotype of African swine fever virus in domestic pigs from Ethiopia Genotyping field strains of African swine fever virus by partial p72 gene characterization Genetic characterization of African swine fever virus isolates from soft ticks at the wildlife/domestic interface in Mozambique and identification of a novel genotype Comparative analysis of African swine fever virus genotypes and serogroups African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity EMBOSS: the European molecular biology open software suite Antigenic diversity of African swine fever viruses Processing and localization of the African swine fever virus CD2v transmembrane protein African swine fever virus African Swine fever. Analyzing the intergenic region between I73R and I329L genes revealed that the strains were identical but contained a deletion of a 33-nucleotide internal repeat sequence compared to ASFV strain Kenya 1950.


id: cord-263469-2w26l80a
author: Garry, Courtney E
date: 2009-09-18
extension: .txt
mime: text/plain
words: 5520
sentence: 241
flesch: 47
cache: cache/cord-263469-2w26l80a.txt
txt: txt/cord-263469-2w26l80a.txt
title:
  Proteomics computational analyses suggest that the bornavirus glycoprotein is a class III viral fusion protein (γ penetrene)

summary:
  Proteomics computational analyses suggest that the structural/functional motifs that characterize class III VFP are located collinearly in BDV G. Structural models were established for BDV G based on the post-fusion structure of a prototypic class III VFP, vesicular stomatitis virus glycoprotein (VSV G). However, many herpesvirus gB, which are class III VFP, contain a furin cleavage domain prior to the extended α helix in domain III and are more heavily glycosylated than VSV G. Therefore, the presence of the furin cleavage site and high glycan content in BDV G does not preclude its putative inclusion in class III.


id: cord-048229-ajlctjeb
author: Golden, Joseph W
date: 2005-05-31
extension: .txt
mime: text/plain
words: 6059
sentence: 265
flesch: 45
cache: cache/cord-048229-ajlctjeb.txt
txt: txt/cord-048229-ajlctjeb.txt
title:
  Neutrophil elastase, an acid-independent serine protease, facilitates reovirus uncoating and infection in U937 promonocyte cells

summary:
  Activity levels in treated cells were so low (in L939 cells, 254 units at 3 h and 231 units at 3 d; in U937 cells, 200 units at 3 h and 115 units at 3 days) that they cannot be visualized on this graph. ISVPs replicated to high yields in treated cells, indicating that the combination of inhibitors was not toxic to U937 cells.


id: cord-278250-dwok857k
author: Li, Heng
date: 2019-08-19
extension: .txt
mime: text/plain
words: 7468
sentence: 314
flesch: 40
cache: cache/cord-278250-dwok857k.txt
txt: txt/cord-278250-dwok857k.txt
title:
  The altered gut virome community in rhesus monkeys is correlated with the gut bacterial microbiome and associated metabolites

summary:
  [16] , and the difference varied more widely between gut viromes than between gut bacterial microbiomes in humans The phylogenetic tree on genus level of gut bacterial microbiome.


id: cord-011880-qlutgfu2
author: Barberis, Abdelheq
date: 2020-07-17
extension: .txt
mime: text/plain
words: 7521
sentence: 288
flesch: 43
cache: cache/cord-011880-qlutgfu2.txt
txt: txt/cord-011880-qlutgfu2.txt
title:
  Full-length genome sequences of the first H9N2 avian influenza viruses isolated in the Northeast of Algeria

summary:
  I. Characteristics of influenza A/Turkey/Wisconsin/1966 virus A brief summary of the epidemiology and genetic relatedness of avian influenza H9N2 virus in birds and mammals in the Middle East and North Africa Avian influenza A (H9N2): computational molecular analysis and phylogenetic characterization of viral surface proteins isolated between 1997 and 2009 from the human population Prevalence of avian respiratory viruses in broiler flocks in Egypt Co-infection with multiple respiratory pathogens contributes to increased mortality rates in Algerian poultry flocks Current situation of H9N2 subtype avian influenza in China Influenza A (H5N1) viruses with A (H9N2) single gene (matrix or PB1) reassortment isolated from Cambodian live bird markets Full-genome analysis of avian influenza virus H9N2 from Bangladesh reveals internal gene reassortments with two distinct highly pathogenic avian influenza viruses Genetic evolution of low pathogenecity H9N2 Avian influenza viruses in Tunisia: acquisition of new mutations First outbreaks and phylogenetic analyses of avian influenza H9N2 viruses isolated from poultry flocks in Morocco Mammalian adaptive mutations of the PA protein of highly pathogenic avian H5N1 influenza virus Highly pathogenic avian influenza A (H9N2) influenza viruses associated with chicken mortality in outbreaks in Algeria Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes Universal primer set for the full-length amplification of all influenza A viruses MEGA X: molecular evolutionary genetics analysis across computing platforms Multiple sequence alignment with the Clustal series of programs Experimental co-infection of infectious bronchitis and low pathogenic avian influenza H9N2 viruses in commercial broiler chickens Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016 Short paper: an experimental study on broiler chicken co-infected with the specimens containing avian influenza (h9 subtype) and infectious bronchitis (4/91 strain) viruses Avian influenza (H9N2) outbreak in Iran Characterization of avian influenza and Newcastle disease viruses from poultry in Libya Genetic and antigenic evolution of H9N2 avian influenza viruses circulating in Egypt between Genesis of avian influenza H9N2 in Bangladesh A brief summary of the epidemiology and genetic relatedness of avian influenza The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties Loss of a single N-linked glycan from the hemagglutinin of influenza virus is associated with resistance to collectins and increased virulence in mice Nlinked glycosylation of the hemagglutinin protein influences virulence and antigenicity of the 1918 pandemic and seasonal H1N1 influenza A viruses Neuraminidase stalk length and additional glycosylation of the hemagglutinin influence the virulence of influenza H5N1 viruses for mice Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases Multiplex polymerase chain reaction for the detection and differentiation of avian influenza viruses and other poultry respiratory pathogens Avian-tohuman transmission of H9N2 subtype influenza


id: cord-267736-rya9w6sh
author: Kang, Xiaoping
title: Development of an ELISA-array for simultaneous detection of five encephalitis viruses
date: 2012-02-27
extension: .txt
mime: text/plain
words: 2993
sentence: 138
flesch: 42
cache: cache/cord-267736-rya9w6sh.txt
txt: txt/cord-267736-rya9w6sh.txt
summary:
  The efficacy of the spotting concentration of the capture antibodies was evaluated by virus culture detection, the proper spotting concentration was determined by a combination of minimized cross reaction and higher signal intensity. To make the assay more amenable to multiple virus detection, the assay protocol was optimized.


id: cord-000990-ci6db90d
author: Guo, Ling
date: 2013-05-26
extension: .txt
mime: text/plain
words: 2776
sentence: 111
flesch: 47
cache: cache/cord-000990-ci6db90d.txt
txt: txt/cord-000990-ci6db90d.txt
title:
  Identification of canine parvovirus with the Q370R point mutation in the VP2 gene from a giant panda (Ailuropoda melanoleuca)

summary:
  Canine parvovirus-A review of epidemiological and diagnostic aspects, with emphasis on type 2c Prevalence and genetic characterization of canine parvoviruses in Korea New approaches for the molecular characterization of canine parvovirus type 2 strains Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus Natural variation of canine parvovirus Canine and feline parvoviruses can use human or feline transferrin receptors to bind, enter, and infect cells Evolution of canine parvovirus: loss and gain of the feline host Immunogenicity of an intranasally administered modified live canine parvovirus type 2b vaccine in pups with maternally derived antibodies Chronological analysis of canine parvovirus type 2 isolates in Japan Evolution of canine parvovirus-a need for new vaccines? Evidence for evolution of canine parvovirus type 2 in Italy First detection of canine parvovirus type 2c in pups with haemorrhagic enteritis in Spain A novel antigenic variant of canine parvovirus from a Vietnamese dog Molecular characterization of canine parvovirus-2 variants circulating in Tunisia Molecular epidemiology of canine parvovirus Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation Occurrence of canine parvovirus type 2c in the United States Serosurvey of infection of parvovirus in giant pandas Serologic survey of giant pandas (Ailuropoda melanoleuca), and domestic dogs and cats in the Wolong Reserve Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation Serosurvey of selected viruses in captive giant pandas (Ailuropoda melanoleuca) in China Analysis of VP2 gene sequences of canine parvovirus isolates in India Molecular Epidemiology and Phylogeny Reveal Complex Spatial Dynamics in Areas Where Canine Parvovirus Is Endemic Surveillance activity for canine parvovirus in Italy Recombination between vaccine and field strains of Canine Parvovirus is revealed by isolation of virus in canine and feline cell cultures Western European epidemiological survey for parvovirus and coronavirus infections in dogs Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats Analysis of canine parvovirus sequences from wolves and dogs isolated in Italy Genetic analysis of VP2 gene of canine parvovirus isolates in Korea Genetic analysis of canine parvovirus type 2c Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses The role of evolutionary intermediates in the host adaptation of canine parvovirus The three-dimensional structure of canine parvovirus and its functional implications Evolutionary reconstructions of the transferrin receptor of Caniforms supports canine parvovirus being a re-emerged and not a novel pathogen in dogs Selective regimen shift and demographic growth increase associated with the emergence of highfitness variants of canine parvovirus Antibody levels and protection to canine parvovirus type 2 Severe parvovirus in a 12-year-old dog that had been repeatedly vaccinated Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c.


id: cord-286256-yol03hid
author: Xu, Tian-min
date: 2020-06-19
extension: .txt
mime: text/plain
words: 1180
sentence: 57
flesch: 51
cache: cache/cord-286256-yol03hid.txt
txt: txt/cord-286256-yol03hid.txt
title:
  Non-optimal effectiveness of convalescent plasma transfusion and hydroxychloroquine in treating COVID-19: a case report

summary:
  Herein, we reported a case receiving combination therapy with CP transfusion and hydroxychloroquine for the first time. The lactic acid and C-reactive protein levels remained high (2.1 mmol/L and 73.23 mg/L, respectively), while the arterial oxyhemoglobin saturation decreased to 86% with a low oxygenation index (OI, 76 mmHg) on day 4 after CP transfusion.


id: cord-307364-j86t65qu
author: Uccellini, Lorenzo
date: 2014-08-08
extension: .txt
mime: text/plain
words: 1925
sentence: 80
flesch: 39
cache: cache/cord-307364-j86t65qu.txt
txt: txt/cord-307364-j86t65qu.txt
title:
  Identification of a novel nidovirus in an outbreak of fatal respiratory disease in ball pythons (Python regius)

summary:
  Total nucleic acids were extracted from snake samples (lung and spleen for snake 1, lung, spleen and esophagus for snake 2, spleen and liver for snake 3, lung and trachea for snake 4) using the EasyMag (bioMérieux, Inc.) platform; Samples from snakes 1 and 2 were depleted of ribosomal RNA (Ribo-Zero™ rRNA Removal, Epibio) and treated with DNAse I (TURBO DNA-free™, Ambion). In situ hybridization confirmed the presence of intracellular, intracytoplasmic viral nucleic acids in the lungs of infected snakes.


id: cord-034467-jh9msz1c
author: Olagoke, Olusola
date: 2020-10-31
extension: .txt
mime: text/plain
words: 2227
sentence: 91
flesch: 42
cache: cache/cord-034467-jh9msz1c.txt
txt: txt/cord-034467-jh9msz1c.txt
title:
  Koalas vaccinated against Koala retrovirus respond by producing increased levels of interferon-gamma

summary:
  Finally, CD4 gene expression levels were significantly lower than CD8β gene expression levels (~ 10 folds) (Fig. 1c) . The results showed that prior to vaccination, IL-8 was expressed at the highest levels, with at least 200-fold greater expression compared to other cytokines, while CD8 mRNA expression was significantly higher than CD4 mRNA expression level.


id: cord-276550-1in7m56w
author: Abdel-Moneim, Ahmed S
date: 2006-09-20
extension: .txt
mime: text/plain
words: 3969
sentence: 166
flesch: 42
cache: cache/cord-276550-1in7m56w.txt
txt: txt/cord-276550-1in7m56w.txt
title:
  S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt

summary:
  Thirty chickens were infected by intraocular instillation of 10 5 EID 50 /100 μl of Infectious bronchitis Virus Taxonomy Coronaviruses in poultry and other birds Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions Identification of amino acids involved in a serotype and neutralization specific epitope within the S1 subunit of avian infectious bronchitis virus Analysis of the serotypespecific epitopes of avian infectious bronchitis virus strains Ark99 and Mass41 Epitopes on the peplomer protein of infectious bronchitis coronavirus strain M41 as defined by monoclonal antibodies A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism Differentiation of infectious bronchitis virus serotypes using the polymerase chain reaction and restriction fragment length polymorphism analysis Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo Location of the amino-acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide Present status of infectious bronchitis in Egypt Studies on the epidemiology and means of control ofinfectious bronchitis disease in chickens in Egypt Isolation and identification of Egypt/Beni-Suef/01 a novel genotype of infectious bronchitis virus Urolithiathis in white commercial egg laying chickens associated with an infectious bronchitis virus Differences inpathogenicity for young chickens among field isolates of infectious bronchitis virus Studies on the prevalence and epidemiology of infectious bronchitis in broiler chickens in El-Minia Governorate The histopathology of infectious bronchitis in fowls infected with a nephrotropic T strain of virus Comparison of the nephropathogenicity of four strains of infectious bronchitis virus The control of avian infectious bronchitis/nephrosis in Australia In vitro characterization and pathogenesis of Egypt/Beni-Suef/01; a novel genotype of infectious bronchitis virus Criteria for examining the immune response to infectious bronchitis virus Some characteristics of isolates of infectious bronchitis virus from commercial vaccines The immune response to infectious bronchitis virus determined by respiratory signs, virus infection, and histopathological lesions Challenge experiments to evaluate cross-protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virus Protection of chickens following live and inactivated virus vaccination against challenge with nephropathogenic infectious bronchitis virus PA/Wolgemuth/98 Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus Vaccination of day-old broilers against infectious bronchitis: Effect of vaccine strain and route of administration Vaccination of one day-old broilersagainst infectious bronchitis by eye drop application or coarse droplet spray and the effect of revaccination by spray Lack of correlation between infectivity, serological response, and challenge results in immunization with an avian infectious bronchitis vaccine Comparison of the spike precursor sequences of coronavirus IBV strains M41 and 6/82with that of IBV Beaudette Phylogeny of antigenic variants of avian coronavirus IBV. Abbreviations CAM, chorioallantoic membrane; ECE, Embryonated chicken eggs; EID, egg infective dose fifty; FITC, fluorescien isothiocynate; HVR, hypervariable region; IBV, infectious bronchitis virus; NCM, nitrocellulose membrane; PBS, phosphate buffer saline; RT, reverse transcriptase; SPF, specific-pathogen-free.


id: cord-271868-giea69b5
author: Firth, Andrew E
date: 2009-09-08
extension: .txt
mime: text/plain
words: 2347
sentence: 90
flesch: 46
cache: cache/cord-271868-giea69b5.txt
txt: txt/cord-271868-giea69b5.txt
title:
  A case for a CUG-initiated coding sequence overlapping torovirus ORF1a and encoding a novel 30 kDa product

summary:
  Finally, we analysed the alignment with MLOGD -a genefinding program which was designed specifically for identifying overlapping coding sequences, and which includes explicit models for sequence evolution in multiply-coding regions [7, 8] ( Figure 1B, panels 7-9) . In this brief report, we describe the bioinformatic discovery of a new, apparently coding, ORF that overlaps the 5' end of the polyprotein coding sequence, ORF1a, in the +2 reading frame.


id: cord-262904-0b0ljjq1
author: Lon, Jerome Rumdon
title: Prediction and evolution of B cell epitopes of surface protein in SARS-CoV-2
date: 2020-10-29
extension: .txt
mime: text/plain
words: 5009
sentence: 203
flesch: 48
cache: cache/cord-262904-0b0ljjq1.txt
txt: txt/cord-262904-0b0ljjq1.txt
summary:
  Coronavirus 2019-nCoV: a brief perspective from the front line The reproductive number of COVID-19 is higher compared to SARS coronavirus Recent advances in B-cell epitope prediction methods Bioinformatics resources and tools for conformational B-cell epitope prediction Comprehensive evolution and molecular characteristics of a large number of SARS-CoV-2 genomes reveal its epidemic trends RCSB Protein Data Bank: biological macromolecular structures enabling research and education in fundamental biology, biomedicine, biotechnology and energy Characterization of the SARS-CoV-2 S protein: biophysical, biochemical, structural, and antigenic analysis The Proteomics Protocols Handbook Sequence and structure-based prediction of eukaryotic protein phosphorylation sites SWISS-MODEL: homology modelling of protein structures and complexes PROCHECK: a program to check the stereochemical quality of protein structures SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling SEPPA 3.0-enhanced spatial epitope prediction enabling glycoprotein antigens ElliPro: a new structure-based tool for the prediction of antibody epitopes Prediction of chain flexibility in proteins Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide The antigenic index: a novel algorithm for predicting antigenic determinants convenient online submission • thorough peer review by experienced researchers in your field • rapid publication on acceptance • support for research data, including large and complex data types • gold Open Access which fosters wider collaboration and increased citations maximum visibility for your research: over 100M website views per year • At BMC, research is always in progress. According to the prediction, there was an outside-in transmembrane helix in 23 residues from position 1214th to position 1236th at the N-terminal of the S protein, which was almost consistent with the study indicating that the transmembrane domain of S protein was at the position from 1213 to 1237th [24] , an inside-out transmembrane helix in 23 residues from position 12th to position 34th at the N-terminal of the E protein.


id: cord-278324-eqqvwwh6
author: Wang, Huanan
date: 2018-09-21
extension: .txt
mime: text/plain
words: 3515
sentence: 147
flesch: 42
cache: cache/cord-278324-eqqvwwh6.txt
txt: txt/cord-278324-eqqvwwh6.txt
title:
  Development of a sensitive and specific xMAP assay for detection of antibodies against infectious laryngotracheitis and bronchitis viruses

summary:
  The simultaneous quantification of ILTV and IBV antibodies were achieved through the interrogation of microspheres by Luminex 200 detection system. . Relevant positive and negative sera have been prepared for the establishment of the singleplex xMAP assay to detect ILTV antibody or IBV antibody in the serum.


id: cord-285253-ik5w4t5e
author: Ali, S Asad
title: Real-world comparison of two molecular methods for detection of respiratory viruses
date: 2011-06-29
extension: .txt
mime: text/plain
words: 3417
sentence: 142
flesch: 44
cache: cache/cord-285253-ik5w4t5e.txt
txt: txt/cord-285253-ik5w4t5e.txt
summary:
  Respiratory viruses are common causes of human disease. However, our data show that molecular diagnostic methods also must be compared to other molecular techniques to assess performance, particularly because of the superior sensitivity of the molecular methods compared to culture and DFA. Detection of respiratory viruses by molecular methods Superiority of reverse-transcription polymerase chain reaction to conventional viral culture in the diagnosis of acute respiratory tract infections in children Evidence of a novel human coronavirus that is associated with respiratory tract disease in infants and young children Clinical evaluation of NucliSENS magnetic extraction and NucliSENS analyte-specific reagents for real-time detection of human metapneumovirus in pediatric respiratory specimens Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections Human rhinoviruses: the cold wars resume Identification of a new human coronavirus Osterhaus AD:


id: cord-289397-a1cuq29o
author: Liu, Jiangning
title: Lycorine reduces mortality of human enterovirus 71-infected mice by inhibiting virus replication
date: 2011-10-27
extension: .txt
mime: text/plain
words: 4084
sentence: 177
flesch: 47
cache: cache/cord-289397-a1cuq29o.txt
txt: txt/cord-289397-a1cuq29o.txt
summary:
  In the saline-treated group, serious muscle necrotic appeared at 3 dpi and lead to paralysis of mice, while the lycorine-treatment (0.4 mg/ kg) obviously reduced the muscle damage caused by EV71 infection (Figure 6B , moderate inflammation were observed in the muscle tissues of lycorine treated mice at 3 dpi and 9 dpi, contrast to the necrotising myositis of placebo-treated mice from 3 dpi to 9 dpi). EV71 infection predominantly leads to hand, foot and mouth disease (HFMD) in children under 6 years old [2, 3] .


id: cord-295207-0p6x4lwx
author: Melnik, Lilia I
title: Peptide inhibition of human cytomegalovirus infection
date: 2011-02-22
extension: .txt
mime: text/plain
words: 4770
sentence: 189
flesch: 38
cache: cache/cord-295207-0p6x4lwx.txt
txt: txt/cord-295207-0p6x4lwx.txt
summary:
  WWIHS score-positive sequences may also interact with hydrophobic surfaces within proteins, and are often sequestered within pre-fusion forms of viral fusion proteins. Previous studies have suggested that synthetic peptides corresponding to or overlapping with sequences in viral fusion proteins that have positive WWIHS scores can sometimes serve as viral entry inhibitors [35] [36]


id: cord-261579-f0prnpsu
author: Eichler, Robert
date: 2006-05-31
extension: .txt
mime: text/plain
words: 2561
sentence: 96
flesch: 43
cache: cache/cord-261579-f0prnpsu.txt
txt: txt/cord-261579-f0prnpsu.txt
title:
  The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C

summary:
  However, for Lassa virus GP it has not yet been shown which sites are actually used and how N-glycosylation affects the characteristics of the glycoprotein in respect to cleavage and transport along the exocytotic pathway. Vero cells were transfected with GP-1 (A) and GP-2 (B) N-glycosylation mutants of Lassa virus glycoprotein.


id: cord-292794-okh6i4l1
author: Wang, Bin
date: 2012-06-27
extension: .txt
mime: text/plain
words: 4791
sentence: 176
flesch: 37
cache: cache/cord-292794-okh6i4l1.txt
txt: txt/cord-292794-okh6i4l1.txt
title:
  Protective efficacy of a broadly cross-reactive swine influenza DNA vaccine encoding M2e, cytotoxic T lymphocyte epitope and consensus H3 hemagglutinin

summary:
  A vaccine based on the extracellular domain of the M2 protein Matrix protein 2 vaccination and protection against influenza viruses, including subtype H5N1 Comparative efficacy of hemagglutinin, nucleoprotein, and matrix 2 protein gene-based vaccination against H5N1 influenza in mouse and ferret Vaxfectin-formulated influenza DNA vaccines encoding NP and M2 viral proteins protect mice against lethal viral challenge Influenza virus M2 protein is an integral membrane protein expressed on the infected-cell surface Heterologous protection against influenza by injection of DNA encoding a viral protein A universal epitope-based influenza vaccine and its efficacy against H5N1 Crossprotection against influenza virus infection by intranasal administration of M2-based vaccine with chitosan as an adjuvant In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs Experimental infection of pigs with the human 1918 pandemic influenza virus Experimental inoculation of pigs with pandemic H1N1 2009 virus and HI cross-reactivity with contemporary swine influenza virus antisera Novel reassortment of Eurasian Avian-like and pandemic/2009 influenza viruses in swine: infectious potential to humans Coadministration of DNA encoding interleukin-6 and hemagglutinin confers protection from influenza virus challenge in mice Coexpressed RIG-I Agonist Enhances Humoral Immune Response to Influenza Virus DNA Vaccine Complete protection against a H5N2 avian influenza virus by a DNA vaccine expressing a fusion protein of H1N1 HA and M2e Immunization of pigs against influenza virus infection by DNA vaccine priming followed by killed-virus vaccine boosting Importance of a prime-boost DNA/protein vaccination to protect chickens against low-pathogenic H7 avian influenza infection Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus The relative immunogenicity of DNA vaccines delivered by the intramuscular needle injection, electroporation and gene gun methods A DNA transfection system for generation of influenza A virus from eight plasmids A simple method of estimating fifty per cent endpoints Sequence comparison between the extracellular domain of M2 protein human and avian influenza A viruses in pigs in southeastern China: potential for genetic reassortment? Genetic evolution of swine influenza A (H3N2) viruses in China from 1970 to Pathogenic and antigenic properties of phylogenetically distinct reassortant H3N2 swine influenza viruses cocirculating in the United States Multiple lineages of antigenically and genetically diverse influenza A virus co-circulate in the United States swine population Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States Evaluation of a protective immunity induced by an inactivated influenza H3N2 vaccine after an intratracheal challenge of pigs Antigenic and molecular heterogeneity in recent swine influenza A (H1N1) virus isolates with possible implications for vaccination policy Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and/or H3N2 subtypes Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs A consensus-hemagglutinin-based DNA vaccine that protects mice against divergent H5N1 influenza viruses A universal influenza


id: cord-261634-vfe1lawl
author: Riddell, Shane
title: The effect of temperature on persistence of SARS-CoV-2 on common surfaces
date: 2020-10-07
extension: .txt
mime: text/plain
words: 4213
sentence: 172
flesch: 49
cache: cache/cord-261634-vfe1lawl.txt
txt: txt/cord-261634-vfe1lawl.txt
summary:
  To verify the reduced recovery on cotton, virus was eluted 5 min after depositing on the cotton, as well as 1 h, the titre of recovered virus after 5 min was similar to that of non-porous surfaces (data not shown) suggesting the process of drying down was a significant factor for cotton material but not from the non-porous surfaces. Work by Lai et al. has shown that stability of SARS virus was enhanced with higher concentrations [25] .


id: cord-000403-vzbh457k
author: Zhang, Weijun
date: 2011-05-30
extension: .txt
mime: text/plain
words: 4280
sentence: 155
flesch: 44
cache: cache/cord-000403-vzbh457k.txt
txt: txt/cord-000403-vzbh457k.txt
title:
  Identification of CD8(+ )cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice

summary:
  A multiple amino acid sequence alignment among different PRRSV M proteins indicates that these two peptides are strongly conserved across multiple PRRSV strains and therefore should be considered for further research. We screened peptides derived from the PRRSV M protein for their ability to induce interferon (IFN)-γ in splenocytes harvested from BALB/ c mice following DNA vaccination and a booster vaccination with recombinant vaccinia virus expressing M protein.


id: cord-279784-o80x8nj7
author: Wu, Yu
date: 2019-10-28
extension: .txt
mime: text/plain
words: 4396
sentence: 211
flesch: 52
cache: cache/cord-279784-o80x8nj7.txt
txt: txt/cord-279784-o80x8nj7.txt
title:
  Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain

summary:
  Porcine epidemic diarrhea virus: an emerging and reemerging epizootic swine virus Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge Novel porcine epidemic diarrhea virus variant with large genomic deletion Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate Development of a live attenuated vaccine candidate against duck Tembusu viral disease Genome organization of porcine epidemic diarrhea virus New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs Isolation and molecular characterization of porcine epidemic diarrhea viruses collected in Japan in 2014 Virus-like particles associated with porcine epidemic diarrhea New variants of porcine epidemic diarrhea cirus, China Genome sequencing and analysis of a novel recombinant porcine epidemic diarrhea virus strain from Henan Porcine epidemic diarrhea vaccine evaluation using a newly isolated strain from Korea New emergence pattern with variant porcine epidemic diarrhea viruses Mucosal and systemic isotypespecific antibody responses and protection in conventional pigs exposed to virulent or attenuated porcine epidemic diarrhoea virus Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate Vaccines for porcine epidemic diarrhea virus and other swine coronaviruses Genetic epidemiology of porcine epidemic diarrhea virus circulating in China in 2012-2017 based on spike gene Trypsin-independent porcine epidemic diarrhea virus US strain with altered virus entry mechanism Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays Isolation and characterization of a variant porcine epidemic diarrhea virus in China A highly pathogenic strain of porcine deltacoronavirus caused watery diarrhea in newborn piglets Oral administration of coated PEDV-loaded microspheres elicited PEDV-specific immunity in weaned piglets Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA Comparative genomic analysis of classical and variant virulent parental/attenuated strains of porcine epidemic diarrhea virus PEDV ORF3 encodes an ion channel protein and regulates virus production Preparation and characterization of an attenuated porcine epidemic diarrhea virus strain by serial passaging Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Porcine epidemic diarrhea (PED) is an acute and highly infectious intestinal disease in pigs; it is caused by porcine epidemic diarrhea virus (PEDV), which has caused enormous economic losses to the global pig industry


id: cord-273122-w9hemznv
author: Jans, Jop
date: 2016-03-22
extension: .txt
mime: text/plain
words: 4739
sentence: 211
flesch: 43
cache: cache/cord-273122-w9hemznv.txt
txt: txt/cord-273122-w9hemznv.txt
title:
  Actin- and clathrin-dependent mechanisms regulate interferon gamma release after stimulation of human immune cells with respiratory syncytial virus

summary:
  PBMCs contain important cells that reflect the immune response against RSV like dendritic cells, monocytes and T cells [4, [24] Previous literature shows that T cells are the main producers of IFN-γ in our model of PBMCs and stimulation with RSV [24] .


id: cord-258935-tatae3hs
author: Lin, Wenyao
date: 2011-06-04
extension: .txt
mime: text/plain
words: 3598
sentence: 129
flesch: 39
cache: cache/cord-258935-tatae3hs.txt
txt: txt/cord-258935-tatae3hs.txt
title:
  A baculovirus dual expression system-based vaccine confers complete protection against lethal challenge with H9N2 avian influenza virus in mice

summary:
  Avian influenza viruses of H9N2 subtype have become highly prevalent in avian species. MDCK cells were used to propagate avian influenza virus and were cultured in minimal essential medium (MEM) containing 10% fetal calf serum at 37°C.


id: cord-000988-79fp75u3
author: Al-Siyabi, Turkiya
title: A cost effective real-time PCR for the detection of adenovirus from viral swabs
date: 2013-06-07
extension: .txt
mime: text/plain
words: 6262
sentence: 259
flesch: 38
cache: cache/cord-000988-79fp75u3.txt
txt: txt/cord-000988-79fp75u3.txt
summary:
  Secondly, the performance characteristics of homogenization may vary between PCR assays and should not be implemented without proper validation [27] . As expected, virus culture-positive specimens had positive PCR results with low Cp values, whereas the virus culture-negative specimens had PCR-positive results with Cp values greater than 30


id: cord-268560-pwps783y
author: Garrido, Jose L
title: EBNA3C interacts with Gadd34 and counteracts the unfolded protein response
date: 2009-12-29
extension: .txt
mime: text/plain
words: 5064
sentence: 188
flesch: 39
cache: cache/cord-268560-pwps783y.txt
txt: txt/cord-268560-pwps783y.txt
summary:
  In B-cells, Gadd34, and EBNA3C are present in a complex with PP1a using microcystin sepharose affinity purification, Using a lymphoblastoid cell line in which EBNA3C protein levels are conditional on hydroxytamoxifen, surprisingly, we found that (i) EBNA3C maintains phosphorylation of eIF2α at serine 51, and (ii) protects against ER stress induced activation of the unfolded protein response as measured by XBP1 (u) versus XBP1(s) protein expression and N-terminal ATF6 cleavage. Since this mutant does not associate with EBNA3C, and is therefore likely not targeted to sites of EBNA3C activity on chromatin, the effect is likely due to swamping of the cell with Gadd34 protein, and sequestration of a positively acting factor such as the histone-acetyl transferase CBP, which is known to be required for full EBNA2 activity at the LMP1 promoter.


id: cord-302170-k2apj469
author: Fang, Peng
date: 2015-10-24
extension: .txt
mime: text/plain
words: 5099
sentence: 218
flesch: 46
cache: cache/cord-302170-k2apj469.txt
txt: txt/cord-302170-k2apj469.txt
title:
  Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR

summary:
  In this study, we reported the validation of reference genes to identify the most suitable internal control gene(s) for the normalization of RT-qPCR data upon viral infection in rice plants. In order to evaluate the expression stability of reference genes, the candidates should be identified first.


id: cord-262599-19aj551d
author: Fongaro, Gislaine
date: 2013-05-28
extension: .txt
mime: text/plain
words: 4315
sentence: 182
flesch: 42
cache: cache/cord-262599-19aj551d.txt
txt: txt/cord-262599-19aj551d.txt
title:
  Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays

summary:
  Water samples were collected, concentrated and HAdV quantified by real-time PCR. The PA value (PFU/mL) equivalence, when compared to ICC-RT-qPCR values (GC/mL) immediately after the concentration of water samples and after a series of decimal dilutions (10 0 to 10 -8 ), is shown in Figure 1 .


id: cord-286332-cdg4im5h
author: van Beurden, Steven J.
date: 2017-06-12
extension: .txt
mime: text/plain
words: 7008
sentence: 286
flesch: 49
cache: cache/cord-286332-cdg4im5h.txt
txt: txt/cord-286332-cdg4im5h.txt
title:
  A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination

summary:
  The most important findings were that (1) murinized IBV contained the correct 5′ S gene sequence (Primer set [F] -with a forward primer located in the IBV 1b gene at a position upstream of the 1b sequence present in p-mIBV and a reverse primer located in the MHV spike gene -resulted in a detectable PCR product for mIBV, but not for IBV or p-mIBV); (2) murizined IBV contained the MHV spike gene at the location of the IBV spike gene (as both primer set [F] and [H] -each with a primer in the MHV spike gene and one in IBV gene 1b [F] or in the M gene [H] -resulted in a detectable PCR product for mIBV, but not for IBV or MHV); (3) the IBV spike gene was absent from the mIBV genome (as primer set [D] targeting the IBV spike gene resulted in a detectable PCR product for IBV, but not for mIBV or MHV(4); recombinant IBV was the result of recombination between genomic RNA from mIBV and RNA transcribed from p-IBV (as primer set [C] -with a forward primer in IBV gene 1b located upstream of the 1b sequence present in p-mIBV and a reverse primer located in the IBV spike gene -resulted in a detectable PCR product for rIBV-wt, but not for mIBV or p-IBV); (5) recombinant IBV contained the IBV spike gene at the location of the MHV-derived spike in mIBV (as both primer set [C] and primer set [E] -each with one primer in the IBV spike gene and the other in IBV ORF


id: cord-271130-6s79q1c1
author: Filoni, Claudia
date: 2017-11-17
extension: .txt
mime: text/plain
words: 6586
sentence: 266
flesch: 42
cache: cache/cord-271130-6s79q1c1.txt
txt: txt/cord-271130-6s79q1c1.txt
title:
  Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)

summary:
  The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. We previously reported on FeLV infections in jaguarundis.


id: cord-284608-ba7wq52t
author: Sias, Catia
date: 2019-03-04
extension: .txt
mime: text/plain
words: 5652
sentence: 237
flesch: 51
cache: cache/cord-284608-ba7wq52t.txt
txt: txt/cord-284608-ba7wq52t.txt
title:
  Alpha, Beta, gamma human PapillomaViruses (HPV) detection with a different sets of primers in oropharyngeal swabs, anal and cervical samples

summary:
  Currently, there are no guidelines recommending HPV oral cavity screening as a mandatory test, and it remains unknown which HPV types should be included in HPV screening programs. In addition, it remains unknown which HPV types should be included in oral cavity screening.


id: cord-293938-40zyv1h8
author: Jonsdottir, Hulda R.
title: Coronaviruses and the human airway: a universal system for virus-host interaction studies
date: 2016-02-06
extension: .txt
mime: text/plain
words: 5536
sentence: 212
flesch: 34
cache: cache/cord-293938-40zyv1h8.txt
txt: txt/cord-293938-40zyv1h8.txt
summary:
  Tracheobronchial HAE cultures recapitulate the primary entry point of human respiratory viruses while the alveolar model allows for elucidation of mechanisms involved in viral infection and pathogenesis in the alveoli. This model is a universal platform to study human respiratory viruses [67]


id: cord-010336-xfzf7ath
author: Lambertz, Ruth Lydia Olga
title: H2 influenza A virus is not pathogenic in Tmprss2 knock-out mice
date: 2020-04-22
extension: .txt
mime: text/plain
words: 3256
sentence: 154
flesch: 56
cache: cache/cord-010336-xfzf7ath.txt
txt: txt/cord-010336-xfzf7ath.txt
summary:
  WT infected mice showed a significant increase in relative lung weights (indicative of hyperemia, pulmonary edema, and/or leukocyte extravasation) compared to Tmprss2 −/− mice that exhibited similar relative lung weights as mockinfected mice (Fig. 2d , e). We did not observe dead animals nor significant body weight loss in infected Tmprss2 −/− mice, whereas WT mice exhibited significant body weight loss and some mortality after infection with PR8_HA(H2) virus (Fig. 2a, b) .


id: cord-290976-dhwlr2ui
author: Lednicky, John A
date: 2013-06-27
extension: .txt
mime: text/plain
words: 7817
sentence: 321
flesch: 51
cache: cache/cord-290976-dhwlr2ui.txt
txt: txt/cord-290976-dhwlr2ui.txt
title:
  Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells

summary:
  Following 2-3 weeks of propagation without antibiotics, the plasmocin-treated cell lines and RPTEC cells were tested by PCR for the presence of mycoplasma DNA using a Takara PCR Mycoplasma Detection kit (Fisher Scientific, Pittsburgh, PA) The Art of animal cell culture for virus isolation Methods for detection and frequency of contamination of fetal calf serum with bovine viral diarrhea virus and antibodies against bovine viral diarrhea virus Bovine viral diarrhea disease associated with a contaminated vaccine Demonstration and genotyping of pestivirus RNA from mammalian cell lines Bovine viral diarrhea virus contamination of nutrient serum, cell cultures and viral vaccines Identification of pestiviruses contaminating cell lines and fetal calf sera Bovine polyomavirus, a frequent contaminant of calf serum Bovine polyomavirus, a frequent contaminant of calf sera A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4 The association of calf serum with the contamination of BHK21 clone 13 suspension cells by a parvovirus serologically related to the minute virus of mice (MVM) Replication of bovine herpesvirus type 4 in human cells in vitro Bovine herpesvirus type 4: a special herpesvirus (review article)


id: cord-253615-qylm0koe
author: Müller, Marcel A
date: 2010-01-15
extension: .txt
mime: text/plain
words: 5828
sentence: 218
flesch: 43
cache: cache/cord-253615-qylm0koe.txt
txt: txt/cord-253615-qylm0koe.txt
title:
  Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane protein

summary:
  Protein ORF 3 might thus constitute a structural protein itself. Moreover, truncated forms were discovered for recombinantly and virally expressed ORF 3a protein which could also be detected in virions [33] .


id: cord-313598-2t40ss6h
author: Ali, Mohsin
title: Throat and nasal swabs for molecular detection of respiratory viruses in acute pharyngitis
date: 2015-10-29
extension: .txt
mime: text/plain
words: 2369
sentence: 85
flesch: 42
cache: cache/cord-313598-2t40ss6h.txt
txt: txt/cord-313598-2t40ss6h.txt
summary:
  Some previous research suggests that flocked throat swabs may be superior to rayon throat swabs for respiratory viral detection. Manitoba: CPHLN Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-PCR assays Self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses Development and evaluation of a flocked nasal midturbinate swab for self-collection in respiratory virus infection diagnostic testing Use of throat swabs or saliva specimens for detection of respiratory viruses in children The Wisconsin upper respiratory symptom survey (WURSS): A new research instrument for assessing the common cold Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay Real-time PCR with an internal control for detection of all known human adenovirus serotypes Evaluation of a new rapid antigen test using immunochromatography for detection of human metapneumovirus in comparison with real-time PCR assay Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by realtime PCR Comparing nose-throat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction Development and assay of RNA transcripts of enterovirus species A to D, rhinovirus species a to C, and human parechovirus: assessment of assay sensitivity and specificity of real-time screening and typing methods Rapid PCR detection of Group A Streptococcus from flocked throat swabs: A retrospective clinical study Comparison of combined nose-throat swabs with nasopharyngeal aspirates for detection of pandemic influenza A/H1N1 2009 virus by real-time reverse transcriptase Swabbing for respiratory viral infections in older patients: a comparison of rayon and nylon flocked swabs Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission •


id: cord-329520-dt0jku7v
author: Hon, Kam LE
date: 2010-07-21
extension: .txt
mime: text/plain
words: 1143
sentence: 61
flesch: 58
cache: cache/cord-329520-dt0jku7v.txt
txt: txt/cord-329520-dt0jku7v.txt
title:
  Influenza or not influenza: Analysis of a case of high fever that happened 2000 years ago in Biblical time

summary:
  Shortly following her recovery, presumbly within minutes, it is described that the woman began to serve Jesus and the disciples, thus making influenza illness highly probable. key: cord-329520-dt0jku7v authors: Hon, Kam LE; Ng, Pak C; Leung, Ting F title: Influenza or not influenza: Analysis of a case of high fever that happened 2000 years ago in Biblical time date: 2010-07-21 journal:


id: cord-322238-8iwljdoi
author: Chen, Qin
date: 2010-08-29
extension: .txt
mime: text/plain
words: 1714
sentence: 69
flesch: 44
cache: cache/cord-322238-8iwljdoi.txt
txt: txt/cord-322238-8iwljdoi.txt
title:
  Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification

summary:
  The sensitivity of LAMP was demonstrated by comparing with PCR tests using serial dilutions (10 -1 to 10 -7 ) of TGEV RNA samples as template. Five other pig viruses were used to confirm the specificity of the LAMP for TGEV detection.


id: cord-305315-0qt7eth0
author: Cao, Liyan
date: 2015-08-18
extension: .txt
mime: text/plain
words: 4258
sentence: 169
flesch: 43
cache: cache/cord-305315-0qt7eth0.txt
txt: txt/cord-305315-0qt7eth0.txt
title:
  Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-β production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway

summary:
  As the key IFN-β transcription factors, we found that dsRNA-induced activation of IFN regulatory factor 3 (IRF-3) was inhibited after PEDV infection, but not nuclear factor-kappaB (NF-κB). In the upstream of IRF-3, TANK-binding kinase 1 (TBK1)-or inhibitor of κB kinase-ε (IKKε)-mediated IFN-β production was not blocked by PEDV, while RIG-I-and its adapter molecule IFN-β promoter stimulator 1 (IPS-1)-mediated IFN-β production were completely inhibited after PEDV infection.


id: cord-282343-cko4curf
author: Cheng, Han
date: 2015-11-24
extension: .txt
mime: text/plain
words: 5076
sentence: 202
flesch: 49
cache: cache/cord-282343-cko4curf.txt
txt: txt/cord-282343-cko4curf.txt
title:
  A parallel genome-wide RNAi screening strategy to identify host proteins important for entry of Marburg virus and H5N1 influenza virus

summary:
  Two genome-wide RNAi screens were performed in parallel against the entry of pseudotyped Marburg viruses and avian influenza virus H5N1 utilizing an HIV-1 based surrogate system, to identify host factors which are important for virus entry. The basic principle of this surrogate assay is based on the following two aspects: (1) AIV and MARV viral envelope glycoproteins (HA and GP, respectively) are necessary and sufficient to mediate virus entry, and (2) these glycoproteins can be efficiently incorporated into HIV-1 viral particles.


id: cord-314954-otc0pc09
author: Liu, Xiaoli
date: 2010-09-13
extension: .txt
mime: text/plain
words: 4283
sentence: 179
flesch: 55
cache: cache/cord-314954-otc0pc09.txt
txt: txt/cord-314954-otc0pc09.txt
title:
  Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus

summary:
  In this study, the C-terminus of DEV UL26 protein (designated UL26c), which contains the whole of UL26.5, was expressed, and the recombinant UL26c protein was used to immunize BALB/c mice to generate monoclonal antibodies (mAb). Western blotting using murine antibody against DEV showed that UL26c could react with DEV antibody ( Figure 1A ), which implied that it had similar antigenicity to native DEV UL26 protein.


id: cord-316142-wg1qwmj1
author: Weiss, Sabrina
date: 2017-09-18
extension: .txt
mime: text/plain
words: 3182
sentence: 136
flesch: 49
cache: cache/cord-316142-wg1qwmj1.txt
txt: txt/cord-316142-wg1qwmj1.txt
title:
  A novel Coltivirus-related virus isolated from free-tailed bats from Côte d’Ivoire is able to infect human cells in vitro

summary:
  The etiology of Colorado tick fever Sequence determination and analysis of the full-length genome of colorado tick fever virus, the type species of genus Coltivirus (family Reoviridae) de Lamballerie X: genus Coltivirus (family Reoviridae): genomic and morphologic characterization of old world and new world viruses In medical microbiology Eyach -an arthorpodborne virus related to Colorado tick fever virus in the Federal Republic of Germany Antibodies against some arboviruses in persons with various neuropathies Isolation of Colorado tick fever virus from rodents in Colorado Ecology of Colorado tick fever Characterization of virus isolates by particle-associated nucleic acid PCR Identification and characterisation of emerging viruses in freeranging bats from sub-Saharan Africa Trimmomatic: a flexible trimmer for Illumina sequence data RAMBO-K: rapid and sensitive removal of background sequences from next generation sequencing data SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing Geneious Basic : an integrated and extendable desktop software platform for the organization and analysis of sequence data Reorganizing the protein space at the universal protein resource (UniProt) MUSCLE: The virus showed closest relationship with two human pathogenic viruses, Colorado tick fever virus and Eyach virus, and was able to infect various human cell lines in vitro.


id: cord-330251-dwjijmwz
author: Volmer, Romain
title: Nucleolar localization of influenza A NS1: striking differences between mammalian and avian cells
date: 2010-03-17
extension: .txt
mime: text/plain
words: 1605
sentence: 67
flesch: 35
cache: cache/cord-330251-dwjijmwz.txt
txt: txt/cord-330251-dwjijmwz.txt
summary:
  Thus, rather than being due to a direct effect of NS1 on nucleolar functions, disruption of the nucleolar morphology in influenza virus infected cells could result from virus induced intracellular stress. The multifunctional nucleolus Cellular stress and nucleolar function Nucleolus: the fascinating nuclear body RNA viruses: hijacking the dynamic nucleolus Nucleolar trafficking is essential for nuclear export of intronless herpesvirus mRNA West Nile virus capsid protein induces p53-mediated apoptosis via the sequestration of HDM2 to the nucleolus Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev The multifunctional NS1 protein of influenza A viruses Nuclear and nucleolar targeting of influenza A virus NS1 protein: striking differences between different virus subtypes Changes in nucleolar morphology and proteins during infection with the coronavirus infectious bronchitis virus Involvement of UL24 in herpes-simplexvirus-1-induced dispersal of nucleolin Phosphorylation of influenza virus nucleoprotein in vivo Identification of cellular interaction partners of the influenza virus ribonucleoprotein complex and polymerase complex using proteomic-based approaches Nucleolar localization of influenza A NS1: striking differences between mammalian and avian cells


id: cord-322937-lakdi3x8
author: Kang, Xiao-ping
date: 2010-06-02
extension: .txt
mime: text/plain
words: 2341
sentence: 83
flesch: 49
cache: cache/cord-322937-lakdi3x8.txt
txt: txt/cord-322937-lakdi3x8.txt
title:
  A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus

summary:
  The assay could simultaneously detect H5N1 avian influenza virus and pandemic H1N1 influenza virus, which could be used for early diagnosis of influenza-like patients and for epidemiological surveillance. Compared with single real-time RT-PCR assay, the duplex real-time RT-PCR assay had the same sensitivity for pandemic H1N1 virus, and about 10-fold more sensitive for H5N1 virus (0.02 TCID 50 ) (Figure 1 ).


id: cord-304058-i8cywew0
author: Pfefferle, Susanne
date: 2009-08-24
extension: .txt
mime: text/plain
words: 9581
sentence: 436
flesch: 50
cache: cache/cord-304058-i8cywew0.txt
txt: txt/cord-304058-i8cywew0.txt
title:
  Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo

summary:
  The characterization of virus proteins can be unreliable if only the protein of interest is studied on its own. In CaCo-2 and HUH7 cells, but not in Vero cells, the variant carrying the ORF 7b deletion had a replicative advantage against the parental virus (4- and 6-fold increase of virus RNA in supernatant, respectively).


id: cord-330777-xcwppaux
author: Esposito, Susanna
date: 2010-04-30
extension: .txt
mime: text/plain
words: 2112
sentence: 69
flesch: 39
cache: cache/cord-330777-xcwppaux.txt
txt: txt/cord-330777-xcwppaux.txt
title:
  Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood

summary:
  The effect of rapid respiratory viral diagnostic testing on antibiotic use in a children's hospital Rapid assays for the diagnosis of influenza A and B viruses in patients evaluated at a large tertiary care children's hospital during two consecutive winter seasons Effect of a rapid influenza diagnosis Impact of human coronavirus infections in otherwise healthy children who attended an Emergency Department The global state of influenza in children Impact of human bocavirus on children and their families Influenza diagnosis and treatment in children: a review of studies on clinically useful tests and antiviral treatment for influenza Antigen-based assays for the identification of influenza virus and respiratory syncytial virus: why and how to use them in pediatrics Comparison of nasopharyngeal nylon flocked swabs with universal transport medium and rayon bud swabs with a sponge reservoir of viral transport medium in the diagnosis of paediatric influenza Nasal swab versus nasopharyngeal aspirate for isolation of respiratory viruses Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children Comparison of four nasal sampling methods for detection of viral pathogens by RT-PCR-A GA(2) LEN project Comparison of flocked and rayon swabs for collection of respiratory epithelial cells from uninfected volunteers and symptomatic patients Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children Dry cotton or flocked respiratory swabs as a simple collection technique for the molecular detection of respiratory viruses using real-time NASBA Comparing nose-throat swabs and nasopharyngeal aspirates collected from children with symptoms for respiratory virus identification using real-time polymerase chain reaction Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood These results suggest that, when an early evaluation of the viral etiology of a respiratory tract infection is needed, parents can collect respiratory secretions at home using pediatric mid-turbinate nasal flocked swabs.


id: cord-337274-1fw0xiin
author: Si, Wei
date: 2010-05-01
extension: .txt
mime: text/plain
words: 2266
sentence: 86
flesch: 52
cache: cache/cord-337274-1fw0xiin.txt
txt: txt/cord-337274-1fw0xiin.txt
title:
  A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus

summary:
  [5] , thus whether CDV vaccine strains are able to provide protection from the current strains of CDV remains a question. The RT-nPCR products were visualized by electrophoresis in a 2% (w/v) agarose gel. RT-nPCR was used to detect the cells infected with CDV vaccine strain, wild-type strain, mixed CDV vaccine and wild-type strains, CPV, CAV, CCV, RV, NDV, and uninfected cells to test its specificity.


id: cord-321756-a7eh4dkb
author: Kwofie, Theophilus B
title: Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana
date: 2012-04-10
extension: .txt
mime: text/plain
words: 3677
sentence: 157
flesch: 42
cache: cache/cord-321756-a7eh4dkb.txt
txt: txt/cord-321756-a7eh4dkb.txt
summary:
  Rapid and quantitative detection of human adenovirus DNA by real-time PCR Comparison of real-time PCR assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children Simultaneous detection of influenza viruses A and B using real-time quantitative PCR Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana Review of infectious diseases Viral respiratory infections and their role as public health problem in tropical countries (review) Respiratory syncytial virus infection in tropical and developing countries Update: respiratory syncytial virus activity-United States, 1996-97 season Epidemiology and aetiology of acute bronchiolitis in Hong Kong infants Viral etiology of acute lower respiratory tract infections in hospitalized young children in Northern Taiwan Respiratory adenoviral infections in children: a study of hospitalized cases in southern Taiwan in 2001-2002 Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections Detection of a broad range of human adenoviruses in respiratory tract samples using a sensitive multiplex real time PCR assay Adenovirus pneumonia in infants and factors for developing bronchiolitis obliterans: a 5-year follow-up Epidemiology and clinical presentation of respiratory syncytial virus infection in a rural area of southern Mozambique Clinical presentation and severity of viral community-acquired pneumonia in young Nepalese children


id: cord-323009-frej2qmb
author: Nakouné, Emmanuel
date: 2013-02-08
extension: .txt
mime: text/plain
words: 2842
sentence: 111
flesch: 40
cache: cache/cord-323009-frej2qmb.txt
txt: txt/cord-323009-frej2qmb.txt
title:
  First introduction of pandemic influenza A/H1N1 and detection of respiratory viruses in pediatric patients in Central African Republic

summary:
  What are the implications for childhood pneumonia of successfully introducing Hib and pneumococcal vaccines in developing countries Childhood pneumonia-progress and challenges Epidemiological and clinical study of viral respiratory tract infections in children from Italy Occurrence of respiratory virus: time, place and person Respiratory viral infections in infants: causes, clinical symptoms, virology, and immunology Influenza in Africa Epidemiological and virological influenza survey in Dakar Pandemic A(H1N1)2009 influenza virus detection by real time RT-PCR: is viral quantification useful? Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses Use of a multiplex PCR/RT-PCR approach to assess the viral causes of influenza-like illnesses in Cambodia during three consecutive dry seasons epidemiology and factors associated with fatal cases CDC: Introduction and transmission of 2009 pandemic influenza A (H1N1) virus-Kenya pandemic influenza A (H1N1) virus outbreak and response-Rwanda Pandemic H1N1 influenza lessons from the southern hemisphere Transmission characteristics of the 2009 H1N1 influenza pandemic: comparison of 8 Southern hemisphere countries Sentinel surveillance for influenza and other respiratory viruses in Côte d'Ivoire Viral etiology of severe pneumonia among Kenyan infants and children Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana Burden of interpandemic influenza in children younger than 5 years: a 25-year prospective study Population-based surveillance for hospitalizations associated with respiratory syncytial virus, influenza virus, and parainfluenza viruses among young children Incidence and clinical characteristics of the infection by the respiratory syncytial virus in children admitted in Santa Casa de São Paulo Hospital Establishing a surveillance network for severe lower respiratory tract infections in Korean infants and young children Virological and clinical characterization of respiratory infections in children attending an emergency department during the first autumn-winter circulation of pandemic A (H1N1) 2009 influenza virus Molecular monitoring of causative viruses in child acute respiratory infection in endemo-epidemic situations in Shanghai Seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay Centers for Disease Control and Prevention: key: cord-323009-frej2qmb authors: Nakouné, Emmanuel; Tricou, Vianney; Manirakiza, Alexandre; Komoyo, Francis; Selekon, Benjamin; Gody, Jean Chrysostome; Victoir, Kathleen; Buchy, Philippe; Kazanji, Mirdad title: First introduction of pandemic influenza A/H1N1 and detection of respiratory viruses in pediatric patients in Central African Republic date: 2013-02-08 journal: Virol J DOI: 10.1186/1743-422x-10-49 sha: doc_id: 323009 cord_uid: frej2qmb BACKGROUND: Acute viral respiratory illnesses in children in sub-Saharan Africa have received relatively little attention, although they are much more frequent causes of morbidity and mortality than in developed countries.


id: cord-316234-vtjsfi2c
author: Sultankulova, Kulyaisan T.
title: New oligonucleotide microarray for rapid diagnosis of avian viral diseases
date: 2017-04-05
extension: .txt
mime: text/plain
words: 4700
sentence: 188
flesch: 40
cache: cache/cord-316234-vtjsfi2c.txt
txt: txt/cord-316234-vtjsfi2c.txt
summary:
  Re: scientific interest in Newcastle disease virus is reviving Molecular and structural bases for the antigenicity of VP2 of infectious bursal disease virus Laboratory manual for isolation and identification of avian pathogens Infectious bronchitis virus in Jordanian chickens: seroprevalence and detection Coronavirus avian infectious bronchitis virus Review of rapid diagnostic tests for influenza Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time PCR Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR-ELISA Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1 A multiplex reverse transcription-polymerase chain reaction assay for Newcastle disease virus and avian pneumovirus (Colorado strain) Avian influenza virus detection and quantitation by real-time RT-PCR Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis Development of a real-time TaqMan RT-PCR assay for the detection of infectious bronchitis virus in chickens, and comparison of RT-PCR and virus isolation Real-time RT-PCR differentiation and quantitation of infectious bursal disease virus strains using dual-labeled fluorescent probes Standard epidemiological methods to understand and improve Apis mellifera health Avian influenza: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Multiplex PCR for typing and subtyping influenza and respiratory syncytial viruses Rapid PCR-based molecular pathotyping of H5 and H7 avian influenza viruses Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus Development of a multiplex real-time polymerase chain reaction for the detection of influenza virus type A including H5 and H9 subtypes Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers Molecular characterization of seven Chinese isolates of infectious bursal disease virus: classical, very virulent and variant strains Infectious bursal disease viruses: molecular differentiation of antigenic subtypes among serotype I viruses Molecular identification of infectious bursal disease virus strains Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction-restriction endonuclease assay Phylogeny of antigenic variants of Avian Coronavirus IBV


id: cord-322738-nvyknvc7
author: Delangue, Julie
title: Viral aetiology influenza like illnesses in Santa Cruz, Bolivia (2010–2012)
date: 2014-02-24
extension: .txt
mime: text/plain
words: 4035
sentence: 138
flesch: 39
cache: cache/cord-322738-nvyknvc7.txt
txt: txt/cord-322738-nvyknvc7.txt
summary:
  The 0-4 yo age group displayed specific epidemiological characteristics with the majority of hRSV infections (infection rate: 23.19%) and a remarkably low proportion of influenza infections: <7.5% compared with~38% in the 5-9 yo age group,~26.5% in the 10-59 yo age classes and~16% in those ≥60 yo. The most surprising items, which deserve investigations on an extended period of time, were the unusually low frequency of influenza infections in the 0-4 yo age group and the complete RSV eclipse in 2011.


id: cord-342117-r2chpw7y
author: Wu, Xinwei
title: Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells
date: 2010-10-14
extension: .txt
mime: text/plain
words: 2933
sentence: 122
flesch: 50
cache: cache/cord-342117-r2chpw7y.txt
txt: txt/cord-342117-r2chpw7y.txt
summary:
  Our data showed that synthetic siRNA against the DEN membrane glycoprotein precursor gene effectively inhibited DEN viral RNA replication and increased C6/36 cell survival rate. Our data showed that synthetic siRNA against the DEN-1 membrane glycoprotein precursor gene effectively inhibited DEN-1 viral RNA replication and increased C6/36 cell survival rate.


id: cord-333349-tsfxpaj6
author: Chai, Weidong
date: 2014-08-08
extension: .txt
mime: text/plain
words: 2525
sentence: 100
flesch: 43
cache: cache/cord-333349-tsfxpaj6.txt
txt: txt/cord-333349-tsfxpaj6.txt
title:
  Elevated dietary zinc oxide levels do not have a substantial effect on porcine reproductive and respiratory syndrome virus (PPRSV) vaccination and infection

summary:
  Pigs receiving higher dietary Zn levels showed a tendency towards higher neutralizing antibody levels after infection, while dietary Zn levels did not substantially influence the number of antiviral IFN-gamma secreting cells (IFN-gamma-SC) or percentages of blood immune cell subsets after infection. Finally, feeding higher dietary Zn levels reduced neither clinical symptoms nor viral loads.


id: cord-341321-paucodwz
author: Finkbeiner, Stacy R
title: Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea
date: 2008-10-14
extension: .txt
mime: text/plain
words: 3620
sentence: 159
flesch: 44
cache: cache/cord-341321-paucodwz.txt
txt: txt/cord-341321-paucodwz.txt
summary:
  In this paper, we report the full sequencing and characterization of the genome of this astrovirus, referred to hereafter as astrovirus MLB1 (AstV-MLB1). Alignment between AstV-MLB1 and other human astroviruses of the highly conserved 52 nt at the ORF1b/ORF2 junction revealed that AstV-MLB1 possessed only 61.5% identity in this region (Fig. 1B) .


id: cord-341168-3gd1w2kn
author: Beer, Christiane
date: 2006-04-02
extension: .txt
mime: text/plain
words: 3200
sentence: 129
flesch: 52
cache: cache/cord-341168-3gd1w2kn.txt
txt: txt/cord-341168-3gd1w2kn.txt
title:
  Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts

summary:
  The high concentration of A-MLV particles bound to large rafts of NIH3T3 cells suggests a role of these microdomains in early A-MLV binding events. Therefore, we conclude that the presence of large rafts in NIH3T3 cells is not a result of A-MLV binding.


id: cord-336870-nirg3269
author: Abebe, Endeshaw Chekol
title: The newly emerged COVID-19 disease: a systemic review
date: 2020-07-08
extension: .txt
mime: text/plain
words: 4167
sentence: 186
flesch: 52
cache: cache/cord-336870-nirg3269.txt
txt: txt/cord-336870-nirg3269.txt
summary:
  The global distribution of COVID-19 cases as of March 12, 2020 WHO situation reports 52 is shown in Fig. The total number of COVID-19 cases is likely to be higher due to the inherent difficulties in identifying and counting mild and asymptomatic cases, especially in those developing countries having poor settings for case detection.


id: cord-319954-lh32rhhe
author: Loo, Liat Hui
date: 2013-09-25
extension: .txt
mime: text/plain
words: 5852
sentence: 237
flesch: 44
cache: cache/cord-319954-lh32rhhe.txt
txt: txt/cord-319954-lh32rhhe.txt
title:
  Evidence for the interaction of the human metapneumovirus G and F proteins during virus-like particle formation

summary:
  The glycans attached to the F protein were resistant to Endo-H-treatment, but PNGaseFtreatment resulted in the formation of a 58 kDa protein species (F58), the expected size for the deglycosylated F protein. Only pCAGGS/F-cmyc transfected cells immunoprecipitated and immunoblotted with anti-cmyc showed the presence of F proteins species corresponding in size to the monomeric and oligomeric F protein ( Figure 1D ).


id: cord-325172-a8ntxnmm
author: Yip, Ming Shum
title: Antibody-dependent infection of human macrophages by severe acute respiratory syndrome coronavirus
date: 2014-05-06
extension: .txt
mime: text/plain
words: 5798
sentence: 190
flesch: 36
cache: cache/cord-325172-a8ntxnmm.txt
txt: txt/cord-325172-a8ntxnmm.txt
summary:
  A first step in understanding SARS pathogenesis Quantitative mRNA expression profiling of ACE 2, a novel homologue of angiotensin converting enzyme CD209L (L-SIGN) is a receptor for severe acute respiratory syndrome coronavirus pH-dependent entry of severe acute respiratory syndrome coronavirus is mediated by the spike glycoprotein and enhanced by dendritic cell transfer through DC-SIGN Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro Anti-severe acute respiratory syndrome coronavirus spike antibodies trigger infection of human immune cells via a pH-and cysteine protease-independent FcgammaR pathway Cleavage of the SARS coronavirus spike glycoprotein by airway proteases enhances virus entry into human bronchial epithelial cells in vitro Monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of infection of feline macrophages Mechanisms and results of the antibody-dependent enhancement of viral infections and role in the pathogenesis of coxsackievirus B-induced diseases Antibody-mediated enhancement of viral disease Antibody-dependent enhancement of viral infection: molecular mechanisms and in vivo implications Mapping epitopes of human Fc gamma RII (CDw32) with monoclonal antibodies and recombinant receptors Severe acute respiratory syndrome Writing Committee of the Second World Health Organization Consultation on Clinical Aspects of Human Infection with Avian Influenza AV An influenza A H1N1 virus revivalpandemic H1N1/09 virus Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia Human infection with a novel avian-origin influenza A (H7N9) virus Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses Multiple organ infection and the pathogenesis of SARS SARScoronavirus replicates in mononuclear cells of peripheral blood (PBMCs) from SARS patients SARS-coronavirus replication in human peripheral monocytes/macrophages Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus.


id: cord-300837-d0a8y5qh
author: Khetawat, Dimple
title: A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System
date: 2010-11-12
extension: .txt
mime: text/plain
words: 9007
sentence: 287
flesch: 38
cache: cache/cord-300837-d0a8y5qh.txt
txt: txt/cord-300837-d0a8y5qh.txt
summary:
  Interestingly, in both the NiV and HeV F glycoprotein mutant series, the higher infectivity of the FΔCt1, FΔCt2 and FΔCt3 bearing pseudotypes in comparison to wild-type was notable, and might be attributed to the greater levels of incorporation of these F glycoproteins into the particles, except in the case of wild-type NiV F and G bearing particles and the reason for this later observation is unclear at present. The series of NiV and HeV F glycoprotein mutants and each wild-type F were expressed by plasmid transfection in HeLa-USU cells, both in the presence and absence of their homologous G glycoprotein partner, and surface proteins were biotin labeled, precipitated with Avidin-agarose, and analyzed by Western blot assay using an F 1 specific antisera ( Figure 5 ).


id: cord-342906-51296y8d
author: Li, Zhiping
title: Aerosolized avian influenza virus by laboratory manipulations
date: 2012-08-06
extension: .txt
mime: text/plain
words: 4501
sentence: 195
flesch: 43
cache: cache/cord-342906-51296y8d.txt
txt: txt/cord-342906-51296y8d.txt
summary:
  Infectious Diseases and Counterterrorism Review of aerosol transmission of influenza A virus Transmission of influenza A in human beings Experimental transmission of influenza virus infection in mice. Immunopath and Infect Dis An experimental model to evaluate the role of transport vehicles as a source of transmission of porcine reproductive and respiratory syndrome virus to susceptible pigs Quantification of airborne influenza and avian influenza virus in a wet poultry market using a filter/real-time qPCR method Inefficient transmission of H5N1 influenza viruses in a ferret contact model Epidemiology and control of virus infections in the laboratory Aerosol technology Aerosol transmission of influenza A virus: A review of new studies Pathogenesis by aerosol infectious diseases: Biological weapons defense.


id: cord-343893-sophqqne
author: Chu, Victor C
title: Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus
date: 2007-02-26
extension: .txt
mime: text/plain
words: 3976
sentence: 177
flesch: 49
cache: cache/cord-343893-sophqqne.txt
txt: txt/cord-343893-sophqqne.txt
summary:
  Kidney cells were washed in 50 ml sterile phosphate buffer saline (PBS) twice via gentle stirring to remove red blood cells. Cell supernatant was mixed with 1 ml of FBS and centrifuged at 1,000 rpm for 2 min. Media were removed and kidney cells were resuspended in 25 ml of M20 media.


id: cord-322024-yrqpq9cf
author: Jevšnik, Monika
date: 2013-02-05
extension: .txt
mime: text/plain
words: 3928
sentence: 143
flesch: 51
cache: cache/cord-322024-yrqpq9cf.txt
txt: txt/cord-322024-yrqpq9cf.txt
title:
  Detection of human coronaviruses in simultaneously collected stool samples and nasopharyngeal swabs from hospitalized children with acute gastroenteritis

summary:
  In 174 of the patients, both stool and NP swab samples were obtained, while only stool sample was obtained from nine children and solely NP swab was available from nine others. In NP swabs respiratory syncytial virus (RSV), influenza viruses A and B (Flu A-B), parainfluenza viruses 1-3 (PIV 1-3), metapneumovirus (hMPV), human bocavirus (HBoVs), adenovirus (AdV) and rhinovirus (hRV)


id: cord-331542-wy068c6o
author: Kong, Ning
title: Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus
date: 2020-04-03
extension: .txt
mime: text/plain
words: 3705
sentence: 164
flesch: 49
cache: cache/cord-331542-wy068c6o.txt
txt: txt/cord-331542-wy068c6o.txt
summary:
  The indirect ELISA and western blot analysis showed that the SE polyclonal antisera had the highest antibody titers against PEDV S1 protein ( Fig. 1a and b) , suggesting that SE protein was the important immunodominant region of S1. The results showed that MAb 2E10 reacted with fragments spanning aa 690 to 736 (SE2) and 707 to 761 (SE3) but not aa 667 to 711 (SE1) or 742 to 798 (SE4), suggesting that the epitope recognized by 2E10 was located in aa 707 to 736 of PEDV S1 protein (Fig. 3b) .


id: cord-310536-u30cufg7
author: Finger, Paula Fonseca
date: 2018-12-12
extension: .txt
mime: text/plain
words: 4667
sentence: 187
flesch: 47
cache: cache/cord-310536-u30cufg7.txt
txt: txt/cord-310536-u30cufg7.txt
title:
  Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis

summary:
  key: cord-310536-u30cufg7 authors: Finger, Paula Fonseca; Pepe, Michele Soares; Dummer, Luana Alves; Magalhães, Carolina Georg; de Castro, Clarissa Caetano; de Oliveira Hübner, Silvia; Leite, Fábio Pereira Leivas; Ritterbusch, Giseli Aparecida; Esteves, Paulo Augusto; Conceição, Fabricio Rochedo title: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis date: 2018-12-12 journal: Virol J DOI: 10.1186/s12985-018-1096-2 sha: doc_id: 310536 cord_uid: u30cufg7 BACKGROUND: The avian infectious bronchitis virus (IBV) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. In order to evaluate the repeatability of the ELISA-rN, three separate batches of recombinant N protein were produced and purified following the methodology described above with distinct purification times, to demonstrate the reproducibility of the recombinant protein expression procedure.


id: cord-348204-365z3qxz
author: Harun, Mohammad Syamsul Reza
date: 2013-11-09
extension: .txt
mime: text/plain
words: 4693
sentence: 179
flesch: 46
cache: cache/cord-348204-365z3qxz.txt
txt: txt/cord-348204-365z3qxz.txt
title:
  Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats

summary:
  Prevalence of feline coronavirus in two cat populations in Malaysia A review of feline infectious peritonitis virus infection: 1963-2008 Feline infectious peritonitis The molecular genetics of feline coronaviruses: comparative sequence analysis of the ORF7a/7b transcription unit of different biotypes Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses Mutation in spike protein cleavage site and pathogenesis of feline coronavirus Natural history of a recurrent feline coronavirus infection and the role of cellular immunity in survival and disease Apoptosis and T-cell depletion during feline infectious peritonitis A possible involvement of TNF-alpha in apoptosis induction in peripheral blood lymphocytes of cats with feline infectious peritonitis Kinetic analysis of a complete poxvirus transcriptome an immediate early class of gene Hepatic transcriptome analysis of hepatitis C virus infection in chimpanzees defines unique gene expression patterns associated with viral clearance Gene-expression changes induced by Feline immunodeficiency virus infection differ in epithelial cells and lymphocytes Viral transcriptome analysis of feline immunodeficiency virus infected cells using second generation sequencing technology A greedy algorithm for aligning DNA sequences Attachment and internalization of feline infectious peritonitis virus in feline blood monocytes and Crandell feline kidney cells Dynamics of gene expression revealed by comparison of serial analysis of gene expression transcript profiles from yeast grown on two different carbon sources Functions, structure, and read-through alternative splicing of feline APOBEC3 genes Increased PD-L1 expression and PD-L1/CD86 ratio on dendritic cells were associated with impaired dendritic cells function in HCV infection Polymorphism in human APOBEC3H affects a phenotype dominant for subcellular localization and antiviral activity Reduced APOBEC3H variant antiviral activities are associated with altered RNA binding activities Feline programmed death and its ligand: characterization and changes with feline immunodeficiency virus infection Upregulation of PD-1 expression on HIV-specific CD8+ T cells leads to reversible immune dysfunction PD-1 and its ligands in tolerance and immunity Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production Regulation of cysteine-rich intestinal protein, a zinc finger protein, by mediators of the immune response Cellular composition and interferon-γ expression of the local inflammatory response in feline infectious peritonitis (FIP) Replication of feline coronaviruses in peripheral blood monocytes Andes virus stimulates interferon-inducible MxA protein expression in endothelial cells West Nile virusinduced cytoplasmic membrane structures provide partial protection against the interferon-induced antiviral MxA protein Identification of five interferon-induced cellular proteins that inhibit west nile virus and dengue virus infections Analysis of ISG expression in chronic hepatitis C identifies viperin as a potential antiviral effector The neutrophil serine protease inhibitor SerpinB1 protects against inflammatory lung injury and morbidity in influenza virus infection SerpinB1 protects the mature neutrophil reserve in the bone marrow Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis The UCSC felCat4 data from the Dec. 2008 catChrV17e draft assembly Mapping and quantifying mammalian transcriptomes by RNA-Seq Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Based on Kal’s Z-test, with False Discovery Rate (FDR) <0.05 and >1.99 fold change on gene expressions, a total of 61 genes were differentially expressed by both samples, where 44 genes were up-regulated and the remainder were down-regulated.


id: cord-339209-oe8onyr9
author: Vasilakis, Nikos
title: Mesoniviruses are mosquito-specific viruses with extensive geographic distribution and host range
date: 2014-05-20
extension: .txt
mime: text/plain
words: 5821
sentence: 222
flesch: 41
cache: cache/cord-339209-oe8onyr9.txt
txt: txt/cord-339209-oe8onyr9.txt
summary:
  Mesoniviridae: a proposed new family in the order Nidovirales formed by a single species of mosquito-borne viruses Examining landscape factors influencing relative distribution of mosquito genera and frequency of virus infection Discovery of the first insect nidovirus, a missing evolutionary link in the emergence of the largest RNA virus genomes An insect nidovirus emerging from a primary tropical rainforest Identification and characterization of genetically divergent members of the newly established family mesoniviridae Molecular biology and pathogenesis of roniviruses A new nidovirus (NamDinh virus NDiV): its ultrastructural characterization in the C6/36 mosquito cell line A new species of mesonivirus from the northern territory, australia Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy Rtips: fast and accurate tools for RNA 2D structure prediction using integer programming A Wolbachia symbiont in Aedes aegypti limits infection with dengue, Chikungunya, and Plasmodium The relative importance of innate immune priming in Wolbachia-mediated dengue interference The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile virus infection Negevirus: a proposed new taxon of insect-specific viruses with wide geographic distribution The footprint of genome architecture in the largest genome expansion in RNA viruses Isolation of a Singh's Aedes albopictus cell clone sensitive to dengue and Chikungunya viruses SMART 7: recent updates to the protein domain annotation resource SMART, a simple modular architecture research tool: identification of signaling domains MUSCLE: multiple sequence alignment with high accuracy and high throughput New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0 TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing SSE: a nucleotide and amino acid sequence analysis platform Mesoniviruses are mosquito-specific viruses with extensive geographic distribution and host range Additional file 5: Figure S5 . The compiled sequences had their relationship to other viruses determined by a BLASTX search.


id: cord-355119-sdg9zdc1
author: Lin, Huixing
date: 2016-04-22
extension: .txt
mime: text/plain
words: 3062
sentence: 120
flesch: 50
cache: cache/cord-355119-sdg9zdc1.txt
txt: txt/cord-355119-sdg9zdc1.txt
title:
  Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge

summary:
  Submit your next manuscript to BioMed Central and we will help you at every step: Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy New variants of porcine epidemic diarrhea virus, China Molecular characterization and phylogenetic analysis of new variants of the porcine epidemic diarrhea virus in Gansu Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain Sequence and phylogenetic analysis of nucleocapsid genes of porcine epidemic diarrhea virus (PEDV) strains in China Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States Complete Genome Sequence of Porcine Epidemic Diarrhea Virus Strain USA/Colorado/2013 from the United States Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences Identification of the epitope region capable of inducing neutralizing antibodies against the porcine epidemic diarrhea virus The GPRLQPY motif located at the carboxyterminal of the spike protein induces antibodies that neutralize Porcine epidemic diarrhea virus Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain Propagation of the virus of porcine epidemic diarrhea in cell culture Detection and genetic diversity of porcine group A rotaviruses in historic (2004) and recent (2011 and 2012) Approximately 80 to 100% of PEDV-infected piglets die within 24 h of being infected with virulent PEDV strains, resulting in tremendous economic losses to the swine industry


id: cord-346574-u28y1ttw
author: Chen, Keyan
date: 2012-08-24
extension: .txt
mime: text/plain
words: 5526
sentence: 225
flesch: 45
cache: cache/cord-346574-u28y1ttw.txt
txt: txt/cord-346574-u28y1ttw.txt
title:
  Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus

summary:
  Additionally, there was a strong agreement between the sandwich enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strips (Kappa = 0.976). The disease is caused by PHE coronavirus (PHE-CoV), which comprises a single strain and is the only known neurotropic CoV affecting pigs


id: cord-340883-zf8jbhdl
author: He, Zhongping
date: 2007-03-27
extension: .txt
mime: text/plain
words: 2894
sentence: 107
flesch: 53
cache: cache/cord-340883-zf8jbhdl.txt
txt: txt/cord-340883-zf8jbhdl.txt
title:
  Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV)

summary:
  There were 92 (33.9%) healthcare workers who acquired SARS, including 51 nurses, 30 physicians, 5 logistics staff, 3 pharmacists and 2 laboratory technicians (one of whom was believed to be infected after handling sputum and stool samples from SARS patients in a diagnostic laboratory). A total of 112 people were infected following exposure to SARS patients in the hospital setting, either as healthcare workers, patients or visitors, and another 62 cases were household contacts of known SARS cases.


id: cord-320617-ucm7wx8b
author: B’Krong, Nguyen Thi Thuy Chinh
date: 2018-04-12
extension: .txt
mime: text/plain
words: 3960
sentence: 166
flesch: 45
cache: cache/cord-320617-ucm7wx8b.txt
txt: txt/cord-320617-ucm7wx8b.txt
title:
  Enterovirus serotypes in patients with central nervous system and respiratory infections in Viet Nam 1997–2010

summary:
  Similarly, in patients with respiratory infection, the clinical significance of EV infection should be interpreted with caution because of the high rate of co-infection with more common respiratory viruses [39] . EV infections are often asymptomatic, but may also result in a diverse spectrum of clinical illness, varying from mild febrile illnesses to severe disease of the cutaneous, gastrointestinal, respiratory, cardiovascular, and central nervous system (CNS)


id: cord-333913-roftm446
author: Holtz, Lori R
title: Klassevirus 1, a previously undescribed member of the family Picornaviridae, is globally widespread
date: 2009-06-24
extension: .txt
mime: text/plain
words: 3143
sentence: 141
flesch: 47
cache: cache/cord-333913-roftm446.txt
txt: txt/cord-333913-roftm446.txt
summary:
  Publish with Bio Med Central and every scientist can read your work free of charge The global burden of diarrhoeal disease A millennium update on pediatric diarrheal illness in the developing world Hospital use by pediatric patients: implications for change A review of viral gastroenteritis A 5-year study of the prevalence and genetic diversity of human caliciviruses associated with sporadic cases of acute gastroenteritis in young children admitted to hospital in Diarrhea etiology in a Children's Hospital Emergency Department: a prospective cohort study Viruses causing gastroenteritis Explaining unexplained diarrhea and associating risks and infections Viral gastroenteritis Virus diversity in a winter epidemic of acute diarrhea in France Picornaviridae: The Viruses and Their Replication Isomura S: Isolation of cytopathic small round viruses with BS-C-1 cells from patients with gastroenteritis Discovery of a novel human picornavirus in a stool sample from a pediatric patient presenting with fever of unknown origin Identification of cardioviruses related to Theiler's murine encephalomyelitis virus in human infections New Saffold cardioviruses in 3 children, Canada Circulation of 3 lineages of a novel Saffold cardiovirus in humans MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis Pan-viral screening of respiratory tract infections in adults with and without asthma reveals unexpected human coronavirus and human rhinovirus diversity Novel human parechovirus from Brazil Genomic characterization of novel human parechovirus type A highly prevalent and genetically diversified Picornaviridae genus in South Asian children Identification of a novel picornavirus related to cosaviruses in a child with acute diarrhea Metagenomic analysis of human diarrhea: viral detection and discovery Pfam: clans, web tools and services Virus Taxonomy Eighth Report of the International Committee on Taxonomy of Viruses Edited by: Fauquet CM Detection of adenoviruses and enteroviruses in polluted waters by nested PCR amplification Viral pollution in the environment and in shellfish: human adenovirus detection by PCR as an index of human viruses Molecular detection of Norwalk-like caliciviruses in sewage Astrovirus detection in wastewater samples Nested multiplex PCR assay for detection of human enteric viruses in shellfish and sewage Improved sensitivity of astrovirus-specific RT-PCR following culture of stool samples in CaCo-2 cells Viral discovery and sequence recovery using DNA microarrays Phylogenetic Anaylsis Using Parsimony (*and other methods Development and application of a one-step low cost procedure to concentrate viruses from seawater samples Many picornaviruses can be detected in human stool such as enteroviruses, polio, Aichi virus, and cardioviruses [12]


id: cord-315688-ba5dus2j
author: He, Lei
date: 2012-08-28
extension: .txt
mime: text/plain
words: 4322
sentence: 166
flesch: 47
cache: cache/cord-315688-ba5dus2j.txt
txt: txt/cord-315688-ba5dus2j.txt
title:
  In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene

summary:
  Transmissible gastroenteritis of pigs Genetic basis for the pathogenesis of transmissible gastroenteritis virus Binding of transmissible gastroenteritis coronavirus to brush border membrane sialoglycoproteins Mucosal immunisation and adjuvants: a brief overview of recent advances and challenges Increased litter survival rates, reduced clinical illness and better lactogenic immunity against TGEV in gilts that were primed as neonates with porcine respiratory coronavirus (PRCV) Cellular immune responses of pigs after primary inoculation with porcine respiratory coronavirus or transmissible gastroenteritis virus and challenge with transmissible gastroenteritis virus RNA silencing: a conserved antiviral immunity of plants and animals Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans RNA interference targeting nucleocapsid protein (C) inhibits classical swine fever virus replication in SK-6 cells Adenovirus-mediated RNA interference against foot-and-mouth disease virus infection both in vitro and in vivo In vitro inhibition of classical swine fever virus replication by siRNAs targeting Npro and NS5B genes Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene Inhibition of porcine transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA Coronavirus gene 7 counteracts host defenses and modulates virus virulence Biochemical aspects of coronavirus replication and virus-host interaction Site-specific alteration of transmissible gastroenteritis virus spike protein results in markedly reduced pathogenicity Transcription regulatory sequences and mRNA expression levels in the coronavirus transmissible gastroenteritis virus Molecular cloning and phylogenetic analysis of ORF7 region of chinese isolate TH-98 from transmissible gastroenteritis virus The 9-kDa hydrophobic protein encoded at the 3' end of the porcine transmissible gastroenteritis coronavirus genome is membraneassociated Sequence analysis of the ORF 7 region of transmissible gastroenteritis viruses isolated in Korea Host cell proteins interacting with the 3' end of TGEV coronavirus genome influence virus replication Transmissible gastroenteritis coronavirus gene 7 is not essential but influences in vivo virus replication and virulence Protection of chickens after live and inactivated virus vaccination against challenge with nephropathogenic infectious bronchitis virus PA/Wolgemuth/98 Safety and efficacy of a modified-live canine coronavirus vaccine in dogs Simultaneously inhibition of HIV and HBV replication through a dual small interfering RNA expression system In vitro inhibition of CSFV replication by retroviral vector-mediated RNA interference A transgenic approach for RNA interferencebased genetic screening in mice A system for stable expression of short interfering RNAs in mammalian cells A large-scale RNAi screen in human cells identifies new components of the p53 pathway Short interfering RNA confers intracellular antiviral immunity in human cells Targeting Marek's disease virus by RNA interference delivered from a herpesvirus vaccine Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs Transmissible gastroenteritis virus induces apoptosis in swine testicular cell lines but not in intestinal enterocytes The viral nucleocapsid protein of transmissible gastroenteritis coronavirus (TGEV) is cleaved by caspase-6 and â��7 during TGEV-induced apoptosis Observation of the cytopathic effect and apoptosis, as well as a cell proliferation assay demonstrated that the three shRNAs were capable of protecting ST cells against TGEV destruction, with high specificity and efficiency.


id: cord-355489-tkvfneje
author: Mendez, Jairo A
title: Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia
date: 2010-09-14
extension: .txt
mime: text/plain
words: 4616
sentence: 194
flesch: 43
cache: cache/cord-355489-tkvfneje.txt
txt: txt/cord-355489-tkvfneje.txt
summary:
  Clinical Investigations Genetic variation and microevolution of dengue 2 virus in Southeast Asia Molecular evolution and distribution of dengue viruses type 1 and 2 in nature Phylogenetic relationships of dengue-2 viruses Molecular epidemiology of dengue-1 and dengue-4 viruses Molecular evolution and phylogeny of dengue-4 viruses Molecular evolution of dengue type 2 virus in Thailand Molecular epidemiology of dengue viruses in Brazil Molecular epidemiology of dengue type 2 virus in Venezuela: evidence for in situ virus evolution and recombination Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus Molecular evolution and phylogeny of dengue type 4 virus in the Caribbean The origin, emergence and evolutionary genetics of dengue virus Molecular epidemiological study of dengue virus type 1 in Taiwan Genotipificación y análisis filogenético de cepas colombianas del virus dengue tipo 2 Molecular epidemiology of dengue virus type 3 in Venezuela Phylogeography and molecular evolution of dengue 2 in the Caribbean basin Introduction of the American/Asian genotype of dengue 2 virus into the Yucatan State of Mexico Dengue virus 3 genotype 1 assosiated with dengue fever and dengue hemorrhagic fever Use of a Short Fragment of the C-Terminal E Gene for Detection and Characterization of Two New Lineages of Dengue Virus 1 in India Simultaneous circulation of genotypes I and III of dengue virus 3 in Colombia Phylogenetic studies reveal existence of multiple lineages of a single genotype of DENV-1 (genotype III) in India during 1956-2007 Dengue virus structural differences that correlate with pathogenesis Effect of age on outcome of secondary dengue 2 infections Burden of symptomatic dengue infection in children at primary school in Thailand: a prospective study Race: a risk factor for dengue hemorrhagic fever Dengue virus evolution and virulence models Microevolution and virulence of dengue viruses Lineage extinction and replacement in dengue type 1 virus populations are due to stochastic events rather than to natural selection Invasion and maintenance of dengue virus type 2 and type 4 in the Americas Molecular epidemiology of dengue 2 viruses in the Philippines: genotype shift and local evolution Molecular evolution of dengue 2 virus in Puerto Rico: Positive selection in the viral envelope accompanies clade reintroduction CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions specific gap penalties and weight matrix choice MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 BEAST: Bayesian evolutionary analysis by sampling trees Estimating the rate of molecular evolution: incorporating non-contemporaneous sequences into maximum likelihood phylogenies Bayesian inference of phylogeny: a non-technical primer Relaxed phylogenetics and dating with confidence RNA virus mutations and fitness for survival The causes and consequences of genetic variation in dengue virus Phylogenetic evidence for adaptive evolution of dengue viruses in nature Selection-driven evolution of emergent dengue virus Patterns of intra-and interhost nonsynonymous variation reveal strong purifying selection in dengue virus


id: cord-327013-gc6o8ou3
author: Kim, Heui Man
date: 2020-07-06
extension: .txt
mime: text/plain
words: 2370
sentence: 104
flesch: 35
cache: cache/cord-327013-gc6o8ou3.txt
txt: txt/cord-327013-gc6o8ou3.txt
title:
  Characterization of neuraminidase inhibitor-resistant influenza virus isolates from immunocompromised patients in the Republic of Korea

summary:
  Influenza Other Respir Viruses Baloxavir: first global approval Incidence of antiviral drug resistance markers among human influenza a viruses in the eastern Mediterranean region Rapid identification of neuraminidase inhibitor resistance mutations in seasonal influenza virus a (H1N1), a (H1N1) 2009, and a (H3N2) subtypes by melting point analysis Summary table of neuraminidase amino acid substitutions associated with reduced inhibition by neuraminidase inhibitors (NAI) Neuraminidase inhibitor resistance in influenza viruses and laboratory testing methods Prolonged shedding of multidrugresistant influenza a virus in an immunocompromised patient Development and evaluation of multiplex real-time RT-PCR assays for seasonal H1pdm09 and avian a/H5 influenza viruses detection Susceptibility of influenza a (H1N1)/pdm2009, seasonal a (H3N2) and B viruses to Oseltamivir in Guangdong Universal primer set for the full-length amplification of all influenza a viruses European Centre for Disease Prevention and Control. The emergence of influenza viruses resistant to anti-influenza drugs is a threat to global public health.


id: cord-308397-kqftn75t
author: Sun, Jia-zeng
date: 2014-12-03
extension: .txt
mime: text/plain
words: 4879
sentence: 202
flesch: 45
cache: cache/cord-308397-kqftn75t.txt
txt: txt/cord-308397-kqftn75t.txt
title:
  MicroRNA miR-320a and miR-140 inhibit mink enteritis virus infection by repression of its receptor, feline transferrin receptor

summary:
  Deep sequencing of small RNAs and analysis of the sequencing data [25] F81 cells cultured in 6-well plates (Costar) were infected with MEV at an input multiplicity (MOI) of 1 pfu/cell. To determine whether the selected miRNAs play a direct role in repression of luciferase expression from pGL3-TfR 3′UTR, 24-well plates seeded with F81 cells at 60-70% confluence were co-transfected with a mixture of pGL3-TfR 3′UTR (4 μg/ml) and pRL-TK vector (4 μg/ml) together with mimics (10 nM).


id: cord-329517-3yn80r9h
author: Yang, Jin-Long
date: 2009-09-15
extension: .txt
mime: text/plain
words: 3582
sentence: 146
flesch: 49
cache: cache/cord-329517-3yn80r9h.txt
txt: txt/cord-329517-3yn80r9h.txt
title:
  Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo

summary:
  Previous studies have examined the distribution of GPV in infected Muscovy ducks by qualitative PCR [9] , including a study that used quantitative PCR [20] . Real-time PCR and qualitative PCR assays


id: cord-314201-6njwigco
author: Maher-Sturgess, Sheryl L
title: Universal primers that amplify RNA from all three flavivirus subgroups
date: 2008-01-24
extension: .txt
mime: text/plain
words: 4628
sentence: 207
flesch: 50
cache: cache/cord-314201-6njwigco.txt
txt: txt/cord-314201-6njwigco.txt
summary:
  The amplification of cDNA was followed by restriction enzyme digestion to identify a range of virus species [7] . Flavivirus detection and taxonomy has recently become more difficult with the determination of the nucleotide sequence of Tamana bat virus (TABV), and Cell fusing agent virus (CFAV)


id: cord-337128-yyz7z0xj
author: Abdel-Moneim, Ahmed S
date: 2009-02-05
extension: .txt
mime: text/plain
words: 3314
sentence: 130
flesch: 38
cache: cache/cord-337128-yyz7z0xj.txt
txt: txt/cord-337128-yyz7z0xj.txt
title:
  Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos

summary:
  key: cord-337128-yyz7z0xj authors: Abdel-Moneim, Ahmed S; Zlotowski, Priscila; Veits, Jutta; Keil, Günther M; Teifke, Jens P title: Immunohistochemistry for detection of avian infectious bronchitis virus strain M41 in the proventriculus and nervous system of experimentally infected chicken embryos date: 2009-02-05 journal: Virol J DOI: 10.1186/1743-422x-6-15 sha: doc_id: 337128 cord_uid: yyz7z0xj BACKGROUND: Infectious bronchitis virus primarily induces a disease of the respiratory system, different IBV strains may show variable tissue tropisms and also affect the oviduct and the kidneys. Xth Wld Vet Poult Assoc Congress American Association of Avian Pathologists Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction-fragment-length polymorphism analysis Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene Detection of infectious bronchitis virus Genetic differences among chicken embryos in response to inoculation with an isolate of infectious bronchitis virus Use of allantoic cells for the detection of avian infectious bronchitis virus Comparative growth kinetic studies on avian infectious bronchitis virus in different systems Infectious bronchitis virus replication in the chicken embryo related cell line A monoclonal antibody-based immunoperoxidase procedure for rapid detection of infectious bronchitis virus in infected tissues Avian infectious bronchitis virus distribution in tissues of chicks The pathogenesis of nephritis in chicks induced by infectious bronchitis virus Tissue tropism of three cloacal isolates and Massachusetts strain of infectious bronchitis virus Gastric gross and microscopic lesions caused by the UNAM-97 variant strain of infectious bronchitis virus after the eighth passage in specific pathogen-free chicken embryos Nephrosis in laying chickens caused by Massachusetts-type infectious bronchitis virus S1 gene sequence analysis of a nephro-pathogenic strain of avian infectious bronchitis virus in Egypt In vitro characterization and pathogenesis of Egypt/Beni-Suef/01; a novel genotype of infectious bronchitis virus Early pathogenesis in chicks of infection with an enterotropic strain of infectious bronchitis virus Detection of in ovo-inoculated infectious bronchitis virus by immunohistochemistry and in situ hybridization with a riboprobe in epithelial cells of the lung and cloacal bursa Tissue distribution of avian infectious bronchitis virus following in ovo inoculation of chicken embryos examined by in situ hybridization with antisense digoxigenin-labeled universal riboprobe The control of avian infectious bronchitis/nephrosis in Australia Establishment of persistent avian infectious bronchitis virus infection in antibodyfree and antibody-positive chickens Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and nonvaccinated flocks in China The quail and chicken intestine have sialyl-galactose sugar chains responsible for the binding of influenza A viruses to human type receptors Properties and developmental regulation of polysialyltransferase activity in the chicken embryo brain Developmental regulation of polysialic acid synthesis in mouse directed by two polysialyltransferases, PST and STX Cell culture methods Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons We thank Katrin Giesow and Gabriele Czerwinski for excellent technical assistance.


id: cord-317773-jdq1d98i
author: Meng, Qing-Wen
title: Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs
date: 2011-12-22
extension: .txt
mime: text/plain
words: 3639
sentence: 164
flesch: 48
cache: cache/cord-317773-jdq1d98i.txt
txt: txt/cord-317773-jdq1d98i.txt
summary:
  The strategy of multi-target miRNAs might be an effective method for inhibiting ALV replication and the acquisition of resistant mutations. In this study, we used RNAi technology to inhibit ALV replication and screened the effective target sites for their ability to inhibit ALV replication at a cellular level.


id: cord-318853-mxyxwkhx
author: Sallie, Richard
date: 2005-08-22
extension: .txt
mime: text/plain
words: 10548
sentence: 301
flesch: 17
cache: cache/cord-318853-mxyxwkhx.txt
txt: txt/cord-318853-mxyxwkhx.txt
title:
  Replicative homeostasis II: Influence of polymerase fidelity on RNA virus quasispecies biology: Implications for immune recognition, viral autoimmunity and other "virus receptor" diseases

summary:
  Of necessity, some receptor affinity studies have used cloned viral protein ligands, an artificial situation that cannot approach the phenotypic complexity of RNA viral protein quasispecies. Viral receptor disease is a unifying hypothesis that may also explain some diseases with well-established, but multi-factorial and apparently unrelated aetiologies – like coronary artery and other vascular diseases – in addition to diseases like schizophrenia that are poorly understood and lack plausible, coherent, pathogenic explanations.


id: cord-329680-ekxsv91t
author: Yu, Yunjia
date: 2019-12-23
extension: .txt
mime: text/plain
words: 6924
sentence: 314
flesch: 53
cache: cache/cord-329680-ekxsv91t.txt
txt: txt/cord-329680-ekxsv91t.txt
title:
  Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways

summary:
  Briefly, MDCK cells were infected with PA pretreated virus (Vir09, NWS, and PR8) at an moi of 0.001 pfu for 1 h at 37°C, and then subjected to plaque assay. 1b and c, PA treatment reduced the virus titers of Vir09, NWS, and PR8 in a dose-dependent manner when used at the concentrations of 6.25-50 μg/ mL. The 50% inhibitory concentration (IC 50 value) of PA for Vir09, NWS, and PR8 was about 6.3 ± 1.3, 3.5 ± 1.4, and 6.1 ± 1.7 μg/mL, respectively (Table 1) .


id: cord-340046-kgbvld0y
author: Houspie, Lieselot
title: Exhaled breath condensate sampling is not a new method for detection of respiratory viruses
date: 2011-03-04
extension: .txt
mime: text/plain
words: 4082
sentence: 175
flesch: 48
cache: cache/cord-340046-kgbvld0y.txt
txt: txt/cord-340046-kgbvld0y.txt
summary:
  key: cord-340046-kgbvld0y authors: Houspie, Lieselot; De Coster, Sarah; Keyaerts, Els; Narongsack, Phouthalack; De Roy, Rikka; Talboom, Ive; Sisk, Maura; Maes, Piet; Verbeeck, Jannick; Van Ranst, Marc title: Exhaled breath condensate sampling is not a new method for detection of respiratory viruses date: 2011-03-04 journal: Virol J DOI: 10.1186/1743-422x-8-98 sha: doc_id: 340046 cord_uid: kgbvld0y BACKGROUND: Exhaled breath condensate (EBC) sampling has been considered an inventive and novel method for the isolation of respiratory viruses. This observation has created a growing interest in the use of EBC as a new sampling method for the screening of respiratory viruses infecting the upper airways.


id: cord-318686-we6pveus
author: Ehlen, Lukas
date: 2016-05-04
extension: .txt
mime: text/plain
words: 5618
sentence: 214
flesch: 46
cache: cache/cord-318686-we6pveus.txt
txt: txt/cord-318686-we6pveus.txt
title:
  Epithelial cell lines of the cotton rat (Sigmodon hispidus) are highly susceptible in vitro models to zoonotic Bunya-, Rhabdo-, and Flaviviruses

summary:
  For virus infection experiments, the medium was removed and cells were inoculated with virus diluted in Optipro serum-free medium (Life Technologies) for 1 h at 37°C. However, in contrast to experimental animals, cell lines are a less laborious model system, less expensive, and can be used in large-scale viral experiments such as in virus isolation trials without the ethical considerations that are involved in animal experiments.


id: cord-337003-7ygcfzii
author: Mehrbod, Parvaneh
date: 2017-11-09
extension: .txt
mime: text/plain
words: 4429
sentence: 198
flesch: 44
cache: cache/cord-337003-7ygcfzii.txt
txt: txt/cord-337003-7ygcfzii.txt
title:
  Association of IFITM3 rs12252 polymorphisms, BMI, diabetes, and hypercholesterolemia with mild flu in an Iranian population

summary:
  Population stratification is related to situations when cases and controls are of different ethnic backgrounds with different genetic compositions, and has been noted as the most important type of the bias in genetic association studies. The mean BMI value was slightly higher among mild flu patients rather than in controls (P value: 0.034).


id: cord-347569-9fvbshz2
author: Balakrishnan, Krishnan Nair
date: 2020-10-27
extension: .txt
mime: text/plain
words: 8637
sentence: 390
flesch: 48
cache: cache/cord-347569-9fvbshz2.txt
txt: txt/cord-347569-9fvbshz2.txt
title:
  Multiple gene targeting siRNAs for down regulation of Immediate Early-2 (Ie2) and DNA polymerase genes mediated inhibition of novel rat Cytomegalovirus (strain All-03)

summary:
  On the other hand, negative control siRNA and untreated groups showed higher viral titer than siRNA treated groups: 4 log 10 TCID50/ml at day 10 p.i and 8 log 10 TCID50/ml at day 18 p.i. Analysis of RCMV ALL-03 infected cells undergoing apoptosis/necrosis upon combination siRNAs treatment were studied using flow cytometry. Besides that, RCMV ALL-03 replication have been reduced by combination siRNAs treatment which indirectly limiting the likelihood of mutation occurrence by inhibiting the growth of virus population carrying the mutated gene for infection.


id: cord-321814-vt6yio6x
author: Pan, Yongfei
title: Isolation and characterization of a variant porcine epidemic diarrhea virus in China
date: 2012-09-12
extension: .txt
mime: text/plain
words: 3665
sentence: 160
flesch: 48
cache: cache/cord-321814-vt6yio6x.txt
txt: txt/cord-321814-vt6yio6x.txt
summary:
  Pig Farming A new coronavirus-like particle associated with diarrhea in swine Virus-like particles associated with porcine epidemic diarrhoea Current aspects in the etiology of viral diarrheas of swine: occurrence of infections with the epizootic viral diarrhea (EVD) virus Light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic diarrhoea virus (EVD): comparison with transmissible gastroenteritis (TGE) virus and porcine rotavirus infections An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Isolation of porcine epidemic diarrhea virus (PEDV) in Korea Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus Chinese-like strain of porcine epidemic diarrhea virus Genetic Characterization of Porcine Epidemic Diarrhea Virus (PEDV) The causative agent of the diarrhea was ultimately identified as porcine epidemic diarrhea virus (PEDV).


id: cord-354138-ps3rzjve
author: Zhao, Fu-Rong
date: 2014-03-14
extension: .txt
mime: text/plain
words: 1510
sentence: 70
flesch: 51
cache: cache/cord-354138-ps3rzjve.txt
txt: txt/cord-354138-ps3rzjve.txt
title:
  Serological report of pandemic (H1N1) 2009 infection among cats in Northeastern China in 2012-02 and 2013-03

summary:
  Influenza A pandemic (H1N1) 2009 virus infection in domestic cat Avian-origin H3N2 canine influenza virus circulating in farmed dogs in Guangdong dog infected with H1N1; study shows virus non pork Serologic evidence of pandemic (H1N1) 2009 infection in dogs Seroepidemiological evidence of avian influenza A virus transmission to pigs in southern China Pandemic and seasonal human influenza virus infections in domestic cats: prevalence, association with respiratory disease, and seasonality patterns Serologic evidence of pandemic influenza virus H1N1 2009 infection in cats in China A serological survey of canine respiratory coronavirus and canine influenza virus in Korean dogs The seroprevalence of pandemic influenza H1N1 (2009) virus in China Serologic survey of the pandemic H1N1 2009 virus in Guangdong Province, China: a cross sectional study Seroepidemiological study of influenza A (H1N1) pdm09 virus following the 2009-2010 wave in Shandong Province Serological report of pandemic (H1N1) 2009 infection among cats in Northeastern China in 2012-02 and 2013-03 Due to frequent cohabitation and close contacts with humans and other animals, cats are uniquely positioned to serve as reservoirs for influenza virus infection both within a household and within the larger farm or rural environment in China


id: cord-325555-be78qely
author: Lyoo, Hey Rhyoung
date: 2015-02-26
extension: .txt
mime: text/plain
words: 4104
sentence: 170
flesch: 40
cache: cache/cord-325555-be78qely.txt
txt: txt/cord-325555-be78qely.txt
title:
  Constant up-regulation of BiP/GRP78 expression prevents virus-induced apoptosis in BHK-21 cells with Japanese encephalitis virus persistent infection

summary:
  It remains a challenge to understand the mechanisms whereby viral persistence is established and maintained, especially viral persistence within a cell or group of cells. We also found that, even in the reduced expression of Bcl-2, siCHOPtransfected cells were highly resistant to the JEV-induced cytopathic effect, as measured by trypan blue exclusion ( Figure 3A and B) .


id: cord-347319-lcuma3eh
author: Ashfaq, Usman A
title: Lysosomotropic agents as HCV entry inhibitors
date: 2011-04-12
extension: .txt
mime: text/plain
words: 2586
sentence: 109
flesch: 44
cache: cache/cord-347319-lcuma3eh.txt
txt: txt/cord-347319-lcuma3eh.txt
summary:
  Hepatitis C virus infection The role of the hepatitis C virus glycoproteins in infection Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey Group atCS: Hepatitis C virus type 1b (II) infection in France and Italy Frequency distribution of hepatitis C virus genotypes in different geographical regions of Pakistan and their possible routes of transmission Chronic hepatitis C virus infection Treatment of chronic hepatitis C with PEGylated interferon and ribavirin Ribavirin and interferon alfa-2b key: cord-347319-lcuma3eh authors: Ashfaq, Usman A; Javed, Tariq; Rehman, Sidra; Nawaz, Zafar; Riazuddin, Sheikh title: Lysosomotropic agents as HCV entry inhibitors date: 2011-04-12 journal: Virol J DOI: 10.1186/1743-422x-8-163 sha: doc_id: 347319 cord_uid: lcuma3eh HCV has two envelop proteins named as E1 and E2 which play an important role in cell entry through two main pathways: direct fusion at the plasma membrane and receptor-mediated endocytosis.


id: cord-351942-u9zpyy29
author: Tan, Bing
date: 2016-11-25
extension: .txt
mime: text/plain
words: 1977
sentence: 98
flesch: 44
cache: cache/cord-351942-u9zpyy29.txt
txt: txt/cord-351942-u9zpyy29.txt
title:
  Isolation and characterization of adenoviruses infecting endangered golden snub-nosed monkeys (Rhinopithecus roxellana)

summary:
  Viral pathogen with increasing importance Adenoviruses in immunocompromised hosts A novel adenovirus species associated with an acute respiratory outbreak in a baboon colony and evidence of coincident human infection Detection of viral agents in fecal specimens of monkeys with diarrhea Cross-species transmission of a novel adenovirus associated with a fulminant pneumonia outbreak in a new world monkey colony New adenovirus species found in a patient presenting with gastroenteritis Multiple cross-species transmission events of human adenoviruses (HAdV) during Hominine evolution Isolation and characterization of adenoviruses persistently shed from the gastrointestinal tract of non-human primates Adenovirus and herpesvirus diversity in free-ranging great apes in the Sangha region of the Republic Of Congo Novel adenoviruses in wild primates: a high level of genetic diversity and evidence of zoonotic transmissions Taxonomy proposal for Old World monkey adenoviruses: characterisation of several non-human, non-ape primate adenovirus lineages Genome analysis of four Old World monkey adenoviruses supports the proposed species classification of primate adenoviruses and reveals signs of possible homologous recombination Rhinopithecus roxellana, The IUCN Red List of Threatened Species Host range, prevalence, and genetic diversity of adenoviruses in bats Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays Isolation and identification of bat viruses closely related to human, porcine, and mink orthoreoviruses Genomic analyses of recombinant adenovirus type 11a in China Structural studies on adenoviruses Homologous recombination in E3 genes of human adenovirus species D Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination RDP2: recombination detection and analysis from sequence alignments Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor Novel bat adenoviruses with an extremely large E3 gene Molecular detection of novel adenoviruses in fecal specimens of captive monkeys with diarrhea in China This work was jointly funded by the National Natural Science Foundation of China The assembled sequence data were uploaded to the GenBank database (http://www.ncbi.nlm.nih.gov/nuccore) at the accession number of KX505867. key: cord-351942-u9zpyy29 authors: Tan, Bing; Wu, Li-Jun; Yang, Xing-Lou; Li, Bei; Zhang, Wei; Lei, Yong-Song; Li, Yong; Yang, Guo-Xiang; Chen, Jing; Chen, Guang; Wang, Han-Zhong; Shi, Zheng-Li title: Isolation and characterization of adenoviruses infecting endangered golden snub-nosed monkeys (Rhinopithecus roxellana) date: 2016-11-25 journal: Virol J DOI: 10.1186/s12985-016-0648-6 sha: doc_id: 351942 cord_uid: u9zpyy29 BACKGROUND: Adenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates.


id: cord-343908-f3v8wi9h
author: Ammayappan, Arun
date: 2008-12-22
extension: .txt
mime: text/plain
words: 2160
sentence: 94
flesch: 51
cache: cache/cord-343908-f3v8wi9h.txt
txt: txt/cord-343908-f3v8wi9h.txt
title:
  Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by recombination

summary:
  key: cord-343908-f3v8wi9h authors: Ammayappan, Arun; Upadhyay, Chitra; Gelb, Jack; Vakharia, Vikram N title: Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by recombination date: 2008-12-22 journal: The complete genome sequence analysis of Ark DPI strain shows striking similarity to the Conn, Gray, JMK, and Ark 99 IBV strains, which were circulating during that time period [1, [19]


id: cord-339744-xrit0w5i
author: Feng, Bo
date: 2017-11-03
extension: .txt
mime: text/plain
words: 6320
sentence: 285
flesch: 45
cache: cache/cord-339744-xrit0w5i.txt
txt: txt/cord-339744-xrit0w5i.txt
title:
  Investigation of antiviral state mediated by interferon-inducible transmembrane protein 1 induced by H9N2 virus and inactivated viral particle in human endothelial cells

summary:
  Thus, it is valuable to reveal the pathogenesis of H9N2 influenza virus infection and the innate immune responses of host to the H9N2 viruses. Moreover, a recent study indicated that H9N2 influenza virus infection induces the expression of IFITM1 in lung, heart and liver in BALB/c mice


id: cord-348876-v55piprx
author: Zhao, Guangyu
date: 2010-01-18
extension: .txt
mime: text/plain
words: 3582
sentence: 144
flesch: 43
cache: cache/cord-348876-v55piprx.txt
txt: txt/cord-348876-v55piprx.txt
title:
  An M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza viruses

summary:
  M2e vaccine designed on sequences of human influenza virus H1, H2 or H3 subtype may not provide the same protection against H5N1 virus leading to human infections [11] . Production of site-specific antibodies to the T cell antigen receptor beta-chain Long-term high-titer neutralizing activity induced by octameric synthetic HIV-1 antigen Roles of adjuvant and route of vaccination in antibody response and protection engendered by a synthetic matrix protein 2-based influenza A virus vaccine in the mouse pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia Emergence and predominance of an H5N1 influenza variant in China Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control Immunization by avian H5 influenza hemagglutinin mutants with altered receptor binding specificity H5N1 outbreaks and enzootic influenza Sequence comparison between the extracellular domain of M2 protein human and avian influenza


id: cord-300434-obvm2en0
author: Ivancic-Jelecki, Jelena
date: 2018-07-18
extension: .txt
mime: text/plain
words: 4819
sentence: 202
flesch: 50
cache: cache/cord-300434-obvm2en0.txt
txt: txt/cord-300434-obvm2en0.txt
title:
  Variability analysis and inter-genotype comparison of human respiratory syncytial virus small hydrophobic gene

summary:
  Codon-based analysis of selective pressure for SH gene sequences was performed using the HyPhy package available on the Datamonkey server. Taxonomy of the order Mononegavirales: update 2016 Two distinct subtypes of human respiratory syncytial virus The small hydrophobic (SH) protein accumulates within lipid-raft structures of the Golgi complex during respiratory syncytial virus infection Protection and mechanism of action of a novel human respiratory syncytial virus vaccine candidate based on the extracellular domain of small hydrophobic protein Demonstration that glycoprotein G is the attachment protein of respiratory syncytial virus Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein Development of a humanized monoclonal antibody (MEDI-493) with potent in vitro and in vivo activity against respiratory syncytial virus Enhanced neutralization of human respiratory syncytial virus by mixtures of monoclonal antibodies to the attachment (G) glycoprotein Antibody responses of children to the C-terminal peptide of the SH protein of respiratory syncytial virus and the immunological characterization of this protein Direct visualization of the small hydrophobic protein of human respiratory syncytial virus reveals the structural basis for membrane permeability The small hydrophobic protein of the human respiratory syncytial virus forms pentameric ion channels Function of the respiratory syncytial virus small hydrophobic protein Genetic variability of human respiratory syncytial virus a strains circulating in Ontario: a novel genotype with a 72 nucleotide G gene duplication Local evolutionary patterns of human respiratory syncytial virus derived from whole-genome sequencing Respiratory syncytial virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene A molecular epidemiological study of human respiratory syncytial virus in Croatia Single-step method of total RNA isolated by acid guanidine phenol extraction Diverse gene junctions of respiratory syncytial virus modulate the efficiency of transcription termination and respond differently to M2-mediated antitermination Variations in transcription termination signals of human respiratory syncytial virus clinical isolates affect gene expression Variations in intergenic region sequences of human respiratory syncytial virus clinical isolates: analysis of effects on transcriptional regulation Genetic and antigenic variability of human respiratory syncytial virus (groups a and B) isolated over seven consecutive seasons in Argentina Circulation patterns of genetically distinct group a and B strains of human respiratory syncytial virus in a community Nikic Hecer A. Early evolution of human respiratory syncytial virus ON1 strains: analysis of the diversity in the C-terminal hypervariable region of glycoprotein gene within the first 3.5 years since their detection Conservation of G-protein epitopes in respiratory syncytial virus (group a) despite broad genetic diversity: is antibody selection involved in virus evolution? Genetic variability among complete human respiratory syncytial virus subgroup a genomes: bridging molecular evolutionary dynamics and epidemiology Positive selection results in frequent reversible amino acid replacements in the G protein gene of human respiratory syncytial virus Genetic variability and molecular evolution of the human respiratory syncytial virus subgroup B attachment G protein Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years Conservation of the respiratory syncytial virus SH gene Inhibition of the human respiratory syncytial virus small hydrophobic protein and structural variations in a bicelle environment Structure and functional dynamics characterization of the ion channel of the human respiratory syncytial virus (hRSV) small hydrophobic protein (SH) transmembrane domain by combining molecular dynamics with excited normal modes Protein-protein interactions of Viroporins in coronaviruses and paramyxoviruses: new targets for antivirals? Not applicable.


id: cord-335865-pibq3iwh
author: Jumat, Muhammad Raihan
date: 2014-11-19
extension: .txt
mime: text/plain
words: 5431
sentence: 206
flesch: 40
cache: cache/cord-335865-pibq3iwh.txt
txt: txt/cord-335865-pibq3iwh.txt
title:
  Imaging analysis of human metapneumovirus-infected cells provides evidence for the involvement of F-actin and the raft-lipid microdomains in virus morphogenesis

summary:
  METHODS: HMPV-infected LLC-MK2 cells were stained with antibodies that recognised the HMPV fusion protein (F protein), attachment protein (G protein) and matrix protein (M protein), and fluorescent probes that detect GM1 within lipid-raft membranes (CTX-B-AF488) and F-actin (Phalloidin-FITC). Cells expressing only the F protein or G protein immunoprecipitated with either anti-FLAG or anti-cmyc respectively showed biotinylated protein species corresponding in size to the G and F proteins respectively.


id: cord-342157-qjyooq68
author: King, Chwan-Chuen
title: Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3
date: 2008-05-21
extension: .txt
mime: text/plain
words: 5869
sentence: 215
flesch: 42
cache: cache/cord-342157-qjyooq68.txt
txt: txt/cord-342157-qjyooq68.txt
summary:
  Sequence diversity and selection pressure of different genomic regions among DENV-3 different genotypes was further examined to understand the global DENV-3 evolution. With different DENV-3 genotypes imported into Taiwan from Southeast Asia and other parts of the world, this virus collection provides an excellent opportunity to examine the sequence diversity of different genes of the full-length DENV-3 viral RNA genome for genotypes other than genotype IV.


id: cord-338372-xsg0j92t
author: Sainz, Bruno
title: Permissiveness of human hepatoma cell lines for HCV infection
date: 2012-01-24
extension: .txt
mime: text/plain
words: 9110
sentence: 274
flesch: 38
cache: cache/cord-338372-xsg0j92t.txt
txt: txt/cord-338372-xsg0j92t.txt
summary:
  Nevertheless, the identification of two new cell lines capable of supporting authentic HCVcc infection, even at reduced levels, expands the current repertoire of cell lines amendable for the study of HCV in vitro and should aid in further elucidating HCV biology and the cellular determinants that modulate HCV infection. Identification of other cell lines able to support HCV infection would not only expand our current repertoire of cell lines available for the study of HCV, but could also prove useful for the identification of cellular determinants of HCV infection.


id: cord-351920-igmb2yfe
author: Oma, Veslemøy Sunniva
date: 2016-06-13
extension: .txt
mime: text/plain
words: 5533
sentence: 242
flesch: 52
cache: cache/cord-351920-igmb2yfe.txt
txt: txt/cord-351920-igmb2yfe.txt
title:
  Bovine coronavirus in naturally and experimentally exposed calves; viral shedding and the potential for transmission

summary:
  General depression and cough were the signs that correlated best with shedding of BCoV RNA, while peak respiratory rate and peak rectal temperature appeared more than a week later than the peak shedding. Although the calves were shedding BCoV RNA 21 days after infection the sentinel animals were not infected.


id: cord-320693-de1lmzl1
author: Hu, Han
title: Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
date: 2019-07-31
extension: .txt
mime: text/plain
words: 5060
sentence: 259
flesch: 48
cache: cache/cord-320693-de1lmzl1.txt
txt: txt/cord-320693-de1lmzl1.txt
summary:
  Residual virus was calculated according to the following formula, residual infectivity = b/a × 100% (a represent TCID50 of residual virus non-treated by the AMPs (control), b represent TCID50 of residual virus after treated by AMPs). Although vaccination can suppress development of the disease, vaccines cannot eliminate virus infection.


id: cord-352361-jh31omg2
author: Nobach, Daniel
date: 2020-01-30
extension: .txt
mime: text/plain
words: 3760
sentence: 170
flesch: 37
cache: cache/cord-352361-jh31omg2.txt
txt: txt/cord-352361-jh31omg2.txt
title:
  No evidence for European bats serving as reservoir for Borna disease virus 1 or other known mammalian orthobornaviruses

summary:
  Spiking of bat RNA with serial dilutions of various orthobornavirus-RNA demonstrated the detection limit of 5000 orthobornavirus copies in 660 ng RNA. Although the bicolored white-toothed shrew has been identified as indigenous reservoir of BoDV-1, other potential reservoirs or animal carriers are still unknown so that further investigations of small mammals including bat species are urgently needed.


id: cord-355906-yeaw9nr8
author: Nedjadi, Taoufik
title: Tackling dengue fever: Current status and challenges
date: 2015-12-09
extension: .txt
mime: text/plain
words: 6825
sentence: 270
flesch: 40
cache: cache/cord-355906-yeaw9nr8.txt
txt: txt/cord-355906-yeaw9nr8.txt
summary:
  Development, characterization and application of monoclonal antibodies against Brazilian dengue virus isolates A simple one-step real-time RT-PCR for diagnosis of dengue virus infection Current advances in dengue diagnosis Reliable classifier to differentiate primary and secondary acute dengue infection based on IgG ELISA Human genetic susceptibility to intracellular pathogens Host genetic susceptibility to severe dengue infection Impact of dengue virus infection on feeding behavior of Aedes aegypti Dengue virus infection of the Aedes aegypti salivary gland and chemosensory apparatus induces genes that modulate infection and blood-feeding behavior A novel amino acid substitution in a voltage gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti Widespread distribution of newly found point mutation in voltage-gated sodium channel in pyrethroid-resistant Aedes aegypti populations in Vietnam Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by allele-specific PCR assay, and its distribution and effect on deltamethrin resistance in Thailand Race: A risk factor for dengue hemorrhagic fever Asymptomatic dengue infection in a Cuban population confirms the protective role of the RR variant of the FcgammaRIIa polymorphism A variant in the CD209 promoter is associated with severity of the disease Dengue and dengue haemorrhagic fever: Implications of host genetics Polymorphisms of the TAP 1 and 2 gene may influence clinical outcome of primary dengue viral infection All serotypes of dengue virus induce HLA-A2 major histocompatibility complex class I promoter activity in human liver cells Two putative subunits of a peptide pump encoded in the human major histocompatibility complex class II region Polymorphisms in the oligoadenylate synthetase gene cluster and its association with clinical outcomes of dengue virus infection Cell type specificity and host genetic polymorphisms influence antibodydependent enhancement of dengue virus infection The 1a, 25-dihydroxy-vitamin D3 reduces dengue virus infection in human myelomonocyte (U937) and hepatic (Huh-7) cell lines and cytokine production in the infected monocytes Association of vitamin D receptor gene polymorphisms with clinical outcomes of dengue virus infection Genome-wide association study identifies susceptibility loci for dengue shock syndrome at MICB and PLCE1 Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family Natural killer cell activation enhances immune pathology and promotes chronic infection by limiting CD8+ T-cell immunity Genetic variants of MICB and PLCE1 and associations with non-severe dengue Host gene expression profiling of dengue virus infection in cell lines and patients Common variants of chemokine receptor gene CXCR3 and its ligands CXCL10 and CXCL11 associated with vascular permeability of dengue infection in peninsular Malaysia Early enhanced expression of interferon-inducible protein-10 (CXCL-10) and other chemokines predicts adverse outcome in severe acute respiratory syndrome Two other compounds have also been shown to qualify as potent inhibitors of dengue virus infection are imino-sugars deoxynojirimycin and castanospermine


id: cord-356064-q56jnhss
author: Bartel, Sebastian
date: 2011-08-01
extension: .txt
mime: text/plain
words: 6185
sentence: 237
flesch: 38
cache: cache/cord-356064-q56jnhss.txt
txt: txt/cord-356064-q56jnhss.txt
title:
  Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides

summary:
  The regulation of eIF-4H may contribute to an enhancement in protein biosynthesis which is required by the virus' need for protein expression. In contrast to the negative control, protein expression is enhanced by an infection with active and UV-inactivated viruses.


id: cord-336510-qzm9wgde
author: Ellermann-Eriksen, Svend
title: Macrophages and cytokines in the early defence against herpes simplex virus
date: 2005-08-03
extension: .txt
mime: text/plain
words: 20078
sentence: 798
flesch: 41
cache: cache/cord-336510-qzm9wgde.txt
txt: txt/cord-336510-qzm9wgde.txt
summary:
  During HSV infection macrophages are activated and possess an increased antiviral potential Occurrence in an adult with pemphigus vulgaris Herpes simplex virus infections Acute hepatitis due to Herpes simplex virus in an adult Disseminated herpes simplex in pregnancy: two cases and a review Herpesvirus hominis type 2 meningoencephalitis following renal transplantation The acquisition of herpes simplex virus during pregnancy Neonatal herpes simplex virus infection in relation to asymptomatic maternal infection at the time of labor Herpes simplex virus infections of women and their offspring: implications for a developed society Neonatal herpes simplex virus infections: is there a role for immunoglobulin in disease prevention and therapy? Type-specific antibody to Herpes Simplex Virus type 2(HSV-2) glycoprotein G in pregnant women, infants exposed to maternal HSV-2 infection at delivery, and infants with Neonatal Herpes Low risk of herpes simplex virus infections in neonates exposed to the virus at the time of vaginal delivery to mothers with recurrent genital herpes simplex virus infections Neonatal herpes simplex infection Safety and efficacy of highdose intravenous acyclovir in the management of neonatal herpes simplex virus infections Natural history of neonatal herpes simplex virus infections in the acyclovir era Advances in the treatment of neonatal herpes simplex infections HSV-2 replication sites, monocyte and lymphocytic cell infection and virion phagocytosis by neutrophils, in vesicular lesions on penile skin.


id: cord-349683-3qdnnwvd
author: Zhang, Xuefeng
date: 2018-07-24
extension: .txt
mime: text/plain
words: 4671
sentence: 164
flesch: 40
cache: cache/cord-349683-3qdnnwvd.txt
txt: txt/cord-349683-3qdnnwvd.txt
title:
  Immunogenicity of adenovirus-vector vaccine targeting hepatitis B virus: non-clinical safety assessment in non-human primates

summary:
  Images were taken using the Olympus BX50 microscope (magnification, × 100) Epidemiology and prevention of hepatitis B virus infection Hepatitis B viral load and risk for liver cirrhosis and hepatocellular carcinoma in the Gambia, West Africa Therapeutic strategies for a functional cure of chronic hepatitis B virus infection Search for a cure for chronic hepatitis B infection: how close are we? HBV therapy and the problem of drug resistance. Therefore, we used the anti-Ad antibody-negative animals in the non-clinical safety assessment test and monitored the levels of anti-Ad antibody in the serum after Ad-HBV administration.


id: cord-349287-mwj2qby4
author: Mackay, Ian M.
title: MERS coronavirus: diagnostics, epidemiology and transmission
date: 2015-12-22
extension: .txt
mime: text/plain
words: 14293
sentence: 570
flesch: 47
cache: cache/cord-349287-mwj2qby4.txt
txt: txt/cord-349287-mwj2qby4.txt
summary:
  There are human MERS cases that do not fall into either category of source and it is unclear if these acquired infection through some entirely separate route, or from cases that escaped diagnosis. However, on occasion, even LRT specimens from MERS cases may initially be negative, only to later become positive by RT-PCR [95] .


id: cord-337636-3yc0ribg
author: Morehouse, Zachary P.
title: A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E
date: 2020-08-25
extension: .txt
mime: text/plain
words: 2981
sentence: 115
flesch: 43
cache: cache/cord-337636-3yc0ribg.txt
txt: txt/cord-337636-3yc0ribg.txt
summary:
  Herein, we are proposing a novel method for viral pathogen detection off swabs as an improvement or alternative to the current PCR based assays commonly used for viral detection This novel two-step, directto-PCR method for viral detection off swabs has shown a lower limit of reliable detection at 1.2 × 10 3 viral copies/mL with 96.30% sensitivity in vitro when screening for HcoV-229E. Herein, we have demonstrated the success of this methodology in vitro and propose it as a novel approach to viral detection that allows for decrease run time in comparison to traditional PCR based viral detection assay protocols, as well as a reduction in the materials needed for successful viral detection.


# ent

id: cord-310536-u30cufg7
sid: 172
eid: 7
type: ORG
entity:
  vírus da bronquite infecciosa determinada pelo sequenciamento de nucleotídeos dos genes n e s1 development


