02_Yanul-1.indd UDC 595.44(477) NEW DATA ON THE RARE SPIDER SPECIES (ARACHNIDA, ARANEAE) FROM KYIV REGION (UKRAINE) V. Yanul1*, V. Terekhova2, N. Polchaninova2 1Taras Schevchenko National University of Kyiv, Volodymyrska st., 64/13, Kyiv, 01033 Ukraine 2V. N. Karazin Kharkiv National University, 4, Maidan Svobody, Kharkiv, 61022 Ukraine Corresponding author *E-mail: vasilyanul2298@gmail.com E-mail n.polcha ninova@karazin.ua E-mail v.terekhova@karazin.ua V. Yanul (https://orcid.org/0000-0003-3057-1149) V. Terekhova (https://orcid.org/0000-0002-6655-9072) N. Polchaninova (https://orcid.org/0000-0003-4605-8788) New Data on the Rare Spider Species (Arachnida, Araneae) from Kyiv Region (Ukraine). Yanul, V., Terekhova,  V., Polchaninova,  N. — Seven rare spider species collected in the Kyiv Region are listed. Eresus moravicus Řezáč, 2008, Parasyrisca arrabonica Szinetár & Eichardt, 2009, and Enoplognatha bryjai Řezáč, 2016 are recorded for the fi rst time from the East European Plain. P. arrabonica and E. bryjai are recorded for the fi rst time from Ukraine; Atypus muralis Bertkau, 1890, A. piceus (Sulzer, 1776), and Mus- telicosa dimidiata (Th orell, 1875) are recorded for the fi rst time from the Kyiv Region. Comments on the geographical distribution and illustrations of four species are provided. K e y w o r d s : spiders, new records, Kyiv Region, Ukraine, East European Plain. Introduction To date, 1086 spider species are known in the fauna of Ukraine (Nentwig et al., 2022). Nevertheless, species distribution within the administrative regions and natural zones is far from being fully understood. According to our estimates, 351 spider species belonging to 32 families have been recorded from Kyiv Region. At the same time, the araneofaunas of well-studied regions are much richer: 573 species are known from the Crimea, 537 species from Zakarpattia, 523 species from Donetsk Region, 460 species from Lviv Region, and 435 species from Kharkiv Region. Th is indicates that the fauna of Kyiv Region needs further study to fi ll the gaps in spider diversity. Th is paper aims to summarize new data on the rare spider species collected in Kyiv Region, to comment on their geographical distribution, and to illustrate the most outstanding records. Zoodiversity, 56(3): 181–188, 2022 DOI 10.15407/zoo2022.03.181 182 V. Yanul, V. Terekhova, N. Polchaninova Material and methods Spiders were collected in the city of Kyiv and the Kyiv Region in 2019–2021 as listed below and are deposited in the personal collections of N. Polchaninova (Kharkiv) and V. Yanul (Kyiv) List of collecting localities mentioned in the text: • Ecological Station ‘Hlyboki Balyky’ 49.96222 N, 31.11889 E. • Onatsky 9.93769 N, 31.04919 E. • Rzhyshchiv Technical School for Construction 49.96611 N, 31.10409 E. • Kozyn 50.223958 N, 30.642077 E. • Kyiv, National Nature Park ‘Holosiivskyi’ 50.375927 N, 30.493395 E. • Vepryk village 50.10606 N, 29.79957 E. We provide photos/drawings of the three species recorded for the fi rst time from the East European Plain, and of another species, which needs an additional illustration of female genitalia. Th e photos were taken by V. Terekhova with the use of the camera Leica DC 300 and the binocular microscope Leica MZ 7.5, the pencil drawings were made by V. Yanul. A list of species Family Atypidae Atypus muralis Bertkau, 1890 M a t e r i a l e x a m i n e d . Kyiv: Hlyboki Balyky, on the path, hand collecting, 24.06.2021, 1 { (N. Polchaninova leg.). D i s t r i b u t i o n . From Central Europe (Nentwig et al., 2022) east to Turkmenistan: Kopetdag (Zonstein, Fet, 1985), and south to Turkey: Muş Province (Özkütük et al., 2015). Ukraine: from Lviv Region and Zakarpattia (Polozhentsev & Akimtseva, 1980; Hirna & Lyesnik, 2014) to Luhansk and Donetsk Regions (Polchninova & Prokoprnko, 2019), and Crimea (Kastrygina, Kovblyuk, 2015); Kyiv Region (fi rst record). Atypus piceus (Sulzer, 1776) M a t e r i a l e x a m i n e d . Kyiv: National Nature Park ‘Holosiivskyi’, hornbeam-oak forest, pitfall traps, 23.06–4.07.2020, 1 {, V. Yanul leg; Kyiv Reg., Onatsky, meadow steppe on the chernozem soil on a top of slope, abandoned pasture, pitfall traps, 28.05‒7.07.2021, 2 { (N. Polchaninova leg.). D i s t r i b u t i o n . A European species known to occur from France to Central European Russia (Nentwig et al., 2022), and also recorded from Iran (Schwendinger, 1990). Ukraine: Lviv and Zakarpattia (Legotay, 1989; Hirna & Lyesnik, 2014) to the north of Luhansk and Donetsk Regions, Kyiv (present data) and Kharkiv (Polchaninova & Prokopenko, 2019), to Cherkasy (Singaevsky, 2010) Regions. Kyiv Region (fi rst record). Family Eresidae Eresus kollari Rossi, 1846 M a t e r i a l e x a m i n e d . Kyiv, vicinity of Kozyn, open dry pine forest on sandy soil, pitfall traps, 17.09‒6.10.2019, 1 { (V. Yanul leg.). D i s t r i b u t i o n . Western Palearctic nemoral-subtropical species, known from Iberian Peninsula to South Siberia (Novosibirsk Region, Russia) (Řezáč et al., 2008); its distribution in Central Asia and East Palearctic is questionable (Nentwig et al., 2022). Eresus moravicus Řezáč, 2008 (fi gs 1‒5) M a t e r i a l e x a m i n e d . Kyiv: Onatsky, meadow steppe on the chernozem soil on the top of slope, abandoned pasture, pitfall traps, 28.05‒7.07.2021, 7 { (N. Polchaninova leg.); ‘Hlyboki Balyky’, on a dirt road, 23.07.2021, 1 } (only a photo is available; photographer: A. Mishta). 183New Data on the Rare Spider Species from Kyiv Region (Ukraine) Figs 1–8. Eresus moravicus: 1 — general appearance, female (photo by A. Mishta), 2 — same, male (photo by V. Terekhova); 3–5 — male palp; Parasyrisca arrabonica: 6–8 — male palp. 3, 6 — palp, prolateral, 4, 7 — same, ventral, 5, 8 — same, retrolateral. Scale bar 0.1 mm. D i s t r i b u t i o n . Th e species is known from Central and Southern Europe (Nentwig et al., 2022). It was also registered in the Ukrainian Carpathians (Zhukovets, Kron (2015): Chornohora Mt., 1971, M. Legotay leg., Ye. Zhukovets det.). Kyiv Region (fi rst record). First record from the East European Plain. 184 V. Yanul, V. Terekhova, N. Polchaninova Family Gnaphosidae Parasyrisca arrabonica Szinetár & Eichardt, 2009 (fi gs 6‒8) M a t e r i a l e x a m i n e d . Kyiv: Rzhyshchiv Technical School for Construction, old abandoned fi eld, mead- ow steppe on sandy soil on a slope, pitfall traps, 8.09‒14.10.2021, 1 { (N. Polchaninova & O. Vasylyuk leg.). D i s t r i b u t i o n . Hungary (Szinetár et al., 2009), Russia: Orenburg Region (Esyunin, Tuneva, 2020), Ukraine: Kyiv Region (fi rst record for Ukraine and the East European Plain). N o t e . A very rare species known from the three localities only. It was described from the sandy grasslands of Hungary, found in the saline lands of Ashchasaiskaya Steppe in the Orenburg Nature Reserve in Russia, and on a sandy slope in Kyiv Region (Ukraine). Figs 9–10. Mustelicosa dimidiata: 9 — epigyne, ventral; Enoplognatha bryjai: 10 — epigyne, dorsal. Scale bars: fi g. 9 0.2 mm; fi g. 10 0.1 mm. 9 10 185New Data on the Rare Spider Species from Kyiv Region (Ukraine) Family Lycosidae Mustelicosa dimidiata (Th orell, 1875) (fi g. 9) M a t e r i a l e x a m i n e d . Kyiv: Kozyn, open dry pine forest on sandy soil, pitfall traps, 21.06‒9.07.2019, 1 {, V. Yanul leg; Rzhyshchiv Technical Schoolfor Building, old abandoned fi eld, meadow steppe on sandy soil on a slope, pitfall traps, 8.09‒14.10.2021, 1 } (N. Polchaninova & O. Vasylyuk leg.). D i s t r i b u t i o n . East European–Central Asian subboreal species; its westernmost known localities were in Rivne and Mykolaiv Regions of Ukraine (Polchaninova et al., 2017; Hirna et al., 2020). In the western part of its range, the species has patchy distribution being associated mainly with grasslands and open pine forests on sandy soils. Its eastern boundary is unclear. According to Yu. Marusik (2018), the species recorded from China, Kazakhstan and Korea as A. albostriata (Grube, 1861) are likely to refer to other species, related to Mustelicosa dimidiata. Nevertheless, there are no recent studies to support this assumption (WSC, 2022). Family Th eridiidae Enoplognatha bryjai Řezáč, 2016 (fi g. 10) M a t e r i a l e x a m i n e d . Kyiv: Vepryk, riparian vegetation (on Carex sp.), hand collecting, between 20.07 and 10.08.2019, 1 } (V. Yanul leg.). D i s t r i b u t i o n . Czech Republic (Řezáč et al., 2016), Bulgaria (Indzhov, 2021). Ukraine: Kyiv Region (fi rst record for Ukraine and the East European Plain). N o t e . Rare species known from riparian biotopes where it inhabits Phragmites aus- tralis, Carex spp., and Typha spp. (Řezáč et al., 2016). It was described from Pannonian swamps in southern Moravia and then was found in Bulgaria. Discussion Accounting for the new records, 357 spider species are currently known from the Kyiv Region. Th ree species, Eresus moravicus, Enoplognatha bryjai, and Parasyrisca arrabonica, are new to the East European Plain; the latter two are new to Ukraine. E. moravicus is absent from the list of Ukrainian spiders in the latest version of the Spiders of Europe (Nentwig et al., 2022), while it is mentioned in the country list in the Catalogue of spiders of the former USSR (Mikhailov, 2021). Th erefore, the current list of spiders of Ukraine includes 1089 species. Th ree Atypus species have been recorded from Ukraine. A. affi nis Eichwald, 1830 is known only from Kremenets (Ternopil Region). Despite the further numerous studies in the western regions of the country, it has not been found since 1830 (Hirna & Lyesnik, 2014). A. piceus was registered in seven regions, and A. muralis in twelve regions. Th e former occurs mainly within the Forest, Wood-and-Steppe, and the north of the Steppe zone of Ukraine, while the latter prevails in the Wood-and-Steppe and Steppe, being rare in the Forest zone. In Europe, A. piceus inhabits dry meadows and slopes with sparce vegetation (Nentwig et al., 2022). Further to the east, it gradually changes its habitat preference to mesic and shadowed biotops. In Ukraine, the species was found in meadow steppe and pinewood on lime- and sandstones in Lviv Region (Hirna & Lyesnik, 2014), on a river beach in the Ternopil Region (Fedoriak et al., 2018), in steppe-meadow and broadleaved forests in Cherkasy Region (Syngayevsky, 2010), in meadow steppe and hornbeam-oak forest in 186 V. Yanul, V. Terekhova, N. Polchaninova Kyiv Region (present data), and only in fl oodplain and mesic oak forests in Donetsk and Kharkiv Regions (Polchaninova, Prokopenko, 2013). A. muralis, on the contrary, chooses drier habitats and occurs in steppe and steppe-like biotops, on the forest edges and in open forests. Th e genus Eresus is also represented in Ukraine by three species: E. kollari, E. moravicus, and E. rotundiceps. An obscure E. rotundiceps Simon, 1873 was described based on two males; its identity was based on the body structure and coloration, while the palp was considered by Simon (1873: 345) to be “similar to that of E. cinnaberinus” (now E. kollari Rossi, 1846). Simon’s type material is deposited in the Muséum national d’Histoire naturelle de Paris, France; the syntype is catalogued as “Specimen MNHN-AR-AR14360. Collection: Simon, Eugéne; Sex: male; Country label: Ukraine; Collector’s name: Waga A” (MNHNP, 2022). Th ereby, the collection date or exact locality is unknown. E. rotundiceps was also recorded from Turkmenistan (Kharitonov, 1932). V. Tyshchenko (1971) synonymized it with E. kollari (as E. niger (Petagna, 1787), cur- rently a nomen dubium, presumably, on the basis of Asian specimens, and other research- ers from Central Asia followed this synonymy (Andreeva, 1976; Nenilin & Pestova, 1985). Nevertheless, P. Lehtinen (1967) left the name valid. Currently, E. rotundiceps is considered a valid nominal species in the WOS (2022); with its range indicated as “Ukraine, Turk- menistan”! However, it was listed as a synonym of E. kollari in the “Catalogue of spiders of the former USSR” (Mikhailov, 2021). Revision of available material will help to resolve this issue. N. Lukyanov (1897) published the fi nding of Eresus kollari and specifi ed the locality as “Kiev, 1848”. Th e border between the Forest and Wood-and-Steppe zones of Ukraine runs a little south of Kyiv. Considering that Kyiv was much smaller in the middle of the 19th century, we can assume that the collecting locality lies in the Forest zone. Th erefore, our fi nding near Kozyn is the fi rst record from the Wood-and-Steppe part of the Kyiv Region and the fi rst one with exact coordinates. Eresus kollari has also been recorded from the Middle Dnipro area within the neighboring Cherkasy Region (Pichka, 1974; Nikitchenko, Repenko, 2001). Now these records are questionable due to the description of E. moravicus (Řezáč et al., 2008). In the collections from the East European Plain, this species had long been neglected and erroneously identified as E. kollari (partly in: Polchaninova, Prokopenko, 2019; Polchaninova, 2021). We examined 45 males and one female from the Cherkasy Re- gion (vicinity of the Kaniv Nature Reserve, collection of E. Singaevsky) and found that all these specimens belonged to E. moravicus. Adult males of E. kollari occur from July (in Ukraine from mid-August) to early October, while those of E. moravicus in May‒June (Řezáč et al., 2008). V. Pichka indicated collecting dates in the Kaniv Na- ture Reserve as April‒October; T. Nikitchenko and L. Repenko did not specify dates or localities. Interestingly, we have never met spring and autumnal males in the same locality, although spiders were trapped by pitfalls from April to October. To clarify distribution patterns of E. kollari vs. E. moravicus, all the material collected in spring and early summer in Ukraine needs to be reassessed. We are grateful to our colleagues S. Indzhov (Bulgaria), T. Szűts (Hungary), and S. Zonstein (Israel) for their comments and confi rmation of the species identifi cation. We also thank E. Singaevsky (Ukraine) for providing material from his personal collection, and C. Hervé (France) for the information on Eresus individuals deposited in the Muséum National d’Histoire Naturelle, Paris. We thank three anonymous reviewers for their comments. 187New Data on the Rare Spider Species from Kyiv Region (Ukraine) References Eichwald, E. 1830. 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