# bib

id: cord-018771-xqlb74px
author: Turinsky, Sebastian
title: Bluttransfusion
date: 2013-04-04
extension: .txt
mime: text/plain
words: 1808
sentence: 104
flesch: 41
cache: cache/cord-018771-xqlb74px.txt
txt: txt/cord-018771-xqlb74px.txt
summary:
  Was muss der Intensivarzt nun tun? Die Gabe von Blut wurde erstmals erfolgreich im 17. Auch ein zweiter Bedside-Test zur Kontrolle zeigt erneut die Blutgruppe B. Da die Blutung klinisch fortschreitet und der Patient in den OP muss, entschließt sich der Intensivarzt zur Transfusion von Erythrozytenkonzentraten der Blutgruppe 0


id: cord-103509-hynnba03
author: Wong, Ten-Tsao
title: A self-assembled protein nanoparticle serving as a one-shot vaccine carrier
date: 2020-09-18
extension: .txt
mime: text/plain
words: 3235
sentence: 119
flesch: 44
cache: cache/cord-103509-hynnba03.txt
txt: txt/cord-103509-hynnba03.txt
summary:
  As shown in figure 1A , when GFP protein with a molecular weight of 27kDa is able to pass through membranes with MWCO of 100kDa, 300kDa and 1000kDa freely, AH3-GFP fusion protein purified from bacterial lysate was prevented from passing through the membrane with an MWCO of 1000kDa. GFP protein has a thermal stable structure that constituted by 11 β-strands and 3 α-helices and some of the loops between strands have been explored as insertion sites for heterologous protein for various purposes (18, 19, 30) .


id: cord-273711-bxijla09
author: Zhao, Zhixun
date: 2012-02-17
extension: .txt
mime: text/plain
words: 4185
sentence: 175
flesch: 46
cache: cache/cord-273711-bxijla09.txt
txt: txt/cord-273711-bxijla09.txt
title:
  RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells

summary:
  Transfection of the siRNA expression cassettes into BHK-21 cells BHK-21 cells were seeded in six-well plates and cultured at 37°C and 5% CO2 overnight. To determine transfection efficiency, we monitored GFP fluorescence intensity of transfected cells using fluorescent microscope analysis.


id: cord-334855-s0ci3r8w
author: Andersen, Petter I.
title: Novel Antiviral Activities of Obatoclax, Emetine, Niclosamide, Brequinar, and Homoharringtonine
date: 2019-10-18
extension: .txt
mime: text/plain
words: 4295
sentence: 200
flesch: 43
cache: cache/cord-334855-s0ci3r8w.txt
txt: txt/cord-334855-s0ci3r8w.txt
summary:
  For testing the production of HSV-2 and EV1 viruses in compound-treated and non-treated RPE cells, the media from the cells were serially (10-fold) diluted, starting from 10 −3 to 10 −8 in serum-free growth media containing 0.2% bovine serum albumin, and applied to a monolayer of A549-Npro cells in 12-well plates. This result indicates that obatoclax and emetine target distinct cellular pathways essential for virus infection.


id: cord-354052-x4ckzw64
author: Li, Chunhua
title: Manipulation of the Porcine Epidemic Diarrhea Virus Genome Using Targeted RNA Recombination
date: 2013-08-02
extension: .txt
mime: text/plain
words: 4940
sentence: 193
flesch: 46
cache: cache/cord-354052-x4ckzw64.txt
txt: txt/cord-354052-x4ckzw64.txt
summary:
  siRNA knockdown of ORF3 gene in PEDV infected cells reduced the number of particles released from the cells [5] . Virus Genes Outbreak of porcine epidemic diarrhea in suckling piglets Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in korea PEDV leader sequence and junction sites PEDV ORF3 encodes an ion channel protein and regulates virus production Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus Propagation of the virus of porcine epidemic diarrhea in cell culture Role of proteases in the release of porcine epidemic diarrhea virus from infected cells Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain:


id: cord-258678-0atfsivf
author: Liu, Hong Yan
title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras
date: 2013-11-07
extension: .txt
mime: text/plain
words: 4800
sentence: 171
flesch: 40
cache: cache/cord-258678-0atfsivf.txt
txt: txt/cord-258678-0atfsivf.txt
summary:
  Unfortunately, these conventional siRNA nanocarriers are unsuitable for chimera delivery, and, in fact, reverse the signature property of chimera, simple formulation for regulatory approval and clinical translation 15, 16 . Although positively charged nanocarriers promote siRNA cell entry, it is well known that they are also quickly cleared by the RES, increase non-specific binding with cells and cytotoxicity 35 .


id: cord-331414-i0oxm5mr
author: Kautz, Tiffany F.
date: 2020-06-19
extension: .txt
mime: text/plain
words: 5071
sentence: 192
flesch: 40
cache: cache/cord-331414-i0oxm5mr.txt
txt: txt/cord-331414-i0oxm5mr.txt
title:
  A Low Fidelity Virus Shows Increased Recombination during the Removal of an Alphavirus Reporter Gene

summary:
  RNA virus recombination primarily occurs when the RdRp switches from the template RNA strand to an acceptor RNA strand (i.e., copy choice recombination) or, less frequently, by ligation of cleaved RNA strands [8] . key: cord-331414-i0oxm5mr authors: Kautz, Tiffany F.; Jaworski, Elizabeth; Routh, Andrew; Forrester, Naomi L. title: A Low Fidelity Virus Shows Increased Recombination during the Removal of an Alphavirus Reporter Gene date: 2020-06-19 journal: Viruses DOI: 10.3390/v12060660 sha: doc_id: 331414 cord_uid: i0oxm5mr Reporter genes for RNA viruses are well-known to be unstable due to putative RNA recombination events that excise inserted nucleic acids.


id: cord-274210-uaaj66xq
author: Ding, Ning
date: 2017-02-28
extension: .txt
mime: text/plain
words: 5165
sentence: 208
flesch: 40
cache: cache/cord-274210-uaaj66xq.txt
txt: txt/cord-274210-uaaj66xq.txt
title:
  Induction of Atypical Autophagy by Porcine Hemagglutinating Encephalomyelitis Virus Contributes to Viral Replication

summary:
  It indicated that the complete autophagy pathway was activated after treatment with rapamycin; although, PHEV infection blocked the smooth of autophagic flow, PHEV replication was also inhibited because of the rapamycin treatment. Neuro-2a cells were infected with PHEV (10 5.43 TCID 50 /mL) in cell cultures with 2% FBS for 1 h. Following a 1 h absorption period, infected cells were incubated in the complete DMEM at 37 • C for the relevant times in the conformity with experimental requirements.


id: cord-324720-acei0pn8
author: Howe, Charles L
title: Inflammatory monocytes damage the hippocampus during acute picornavirus infection of the brain
date: 2012-03-09
extension: .txt
mime: text/plain
words: 5542
sentence: 242
flesch: 47
cache: cache/cord-324720-acei0pn8.txt
txt: txt/cord-324720-acei0pn8.txt
summary:
  Uninfected mice, untreated Theiler's murine encephalomyelitis virus (TMEV)-infected mice (No Tx), and infected mice treated with RB6-8C5 to deplete inflammatory monocytes and neutrophils (RB6 Tx) were assessed in the Morris water maze starting at 14 dpi. Phenotypic differences defined and direct ex vivo antigen presentation to myelin basic protein-reactive CD4+ T cells compared Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response Characterization of the five novel Ly-6 superfamily members encoded in the MHC, and detection of cells expressing their potential ligands Inflammatory monocytes damage the hippocampus during acute picornavirus infection of the brain


id: cord-002320-m99amd4y
author: Mathur, Kalika
date: 2016-11-30
extension: .txt
mime: text/plain
words: 5683
sentence: 236
flesch: 48
cache: cache/cord-002320-m99amd4y.txt
txt: txt/cord-002320-m99amd4y.txt
title:
  Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity

summary:
  Taken together, the current study has identified CHIKV proteins nsP2 and nsP3 to exhibit RNAi suppressor activity in the in-vitro system that was further demonstrated in its natural hosts, namely, an Aedes and mammalian cell line. RNA-based antiviral immunity Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants RNAi function, diversity, and loss in the fungal kingdom Mosquito RNAi is the major innate immune pathway controlling arbovirus infection and transmission Dicing of viral replication intermediates during silencing of latent Drosophila viruses Small silencing RNAs: an expanding universe The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster The rest is silence Dicing and slicing: the core machinery of the RNA interference pathway


id: cord-345651-admlzeu4
author: Wang, Gang
date: 2019-03-30
extension: .txt
mime: text/plain
words: 6244
sentence: 195
flesch: 40
cache: cache/cord-345651-admlzeu4.txt
txt: txt/cord-345651-admlzeu4.txt
title:
  The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets

summary:
  The 43.5-kilodalton band of TGEV N protein ( Figure 2C ) and the marker mutation at position 4553 in the TGEV genome ( Figures 1B and 2D ) confirmed the recovery of the TGEV-GFP recombinant virus. The 43.5-kilodalton band of TGEV N protein ( Figure 2C ) and the marker mutation at position 4553 in the TGEV genome ( Figures 1B and 2D ) confirmed the recovery of the TGEV-GFP recombinant virus.


id: cord-317142-qd61qvch
author: Muramatsu, Tomonari
date: 2013-03-27
extension: .txt
mime: text/plain
words: 6370
sentence: 237
flesch: 53
cache: cache/cord-317142-qd61qvch.txt
txt: txt/cord-317142-qd61qvch.txt
title:
  Autoprocessing mechanism of severe acute respiratory syndrome coronavirus 3C‐like protease (SARS‐CoV 3CL (pro)) from its polyproteins

summary:
  They used two types of pro-3CL pro molecules with an N-terminal pro-sequence of six amino acids, accompanied by CFP and YFP at the N-and C-termini, respectively: one contains an active (wild-type) protease site and and inactive N-terminal processing site (Q-1E) (enzyme), and the other contains an inactive protease site (C145A) and an active (wild-type) N-terminal processing site (substrate). This assumption is supported by the difference in the inhibitory effects of the N-terminal pro-sequences of the two substrates, the pro-3CL(C145A) and GFP substrate, on the N-terminally processed enzyme/proenzyme (WT or Q306N; Fig. 4 ).


id: cord-353467-wbtzvm4i
author: Lambert, Carsten
title: Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles
date: 2004-12-05
extension: .txt
mime: text/plain
words: 6333
sentence: 222
flesch: 42
cache: cache/cord-353467-wbtzvm4i.txt
txt: txt/cord-353467-wbtzvm4i.txt
summary:
  Among the three related large L, middle M, and small S envelope proteins, L has been shown to play the key role in receptor recognition and cell attachment (Le Seyec et al., 1999; Neurath et al., 1986) . Because HBV empty envelope particles, built from the S protein, provide a safe antigen delivery system, their use as a carrier for the presentation of various antigens is a long established practice.


id: cord-332881-mkm4ygh6
author: Kirchhoff, Jana
date: 2014-02-18
extension: .txt
mime: text/plain
words: 6411
sentence: 280
flesch: 46
cache: cache/cord-332881-mkm4ygh6.txt
txt: txt/cord-332881-mkm4ygh6.txt
title:
  Three viruses of the bovine respiratory disease complex apply different strategies to initiate infection

summary:
  Respiratory epithelial cells were also refractory to infection by BRSV. Another culture system for differentiated respiratory epithelial cells are precision-cut lung slices (PCLS), where the epithelial cells are maintained in their original setting.


id: cord-001123-n2e4s7bu
author: Lin, Yue-Zhi
date: 2013-11-22
extension: .txt
mime: text/plain
words: 5900
sentence: 221
flesch: 45
cache: cache/cord-001123-n2e4s7bu.txt
txt: txt/cord-001123-n2e4s7bu.txt
title:
  The Soluble Form of the EIAV Receptor Encoded by an Alternative Splicing Variant Inhibits EIAV Infection of Target Cells

summary:
  Therefore, the present study had three objectives: 1) to identify alternative splicing variants for ELR1, 2) to determine whether any of these variants encode soluble ELR1 and 3) to characterize the roles of any soluble forms of the receptor in EIAV infection. Horses and related experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee (IACUC) of the Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences. The expression of soluble ELR1 (sELR1) was examined by Western blot, flow cytometry and confocal microscopy.


id: cord-338811-2bi2edcw
author: Lennemann, Nicholas J.
title: Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells
date: 2020-09-25
extension: .txt
mime: text/plain
words: 6682
sentence: 264
flesch: 38
cache: cache/cord-338811-2bi2edcw.txt
txt: txt/cord-338811-2bi2edcw.txt
summary:
  Live-cell imaging of CVB infected cells The data was collected from two independent experiments, with four separate fields/experiment. Live-cell imaging of CVB infected cells expressing these reporters allowed for the real-time visualization of virus-induced changes to the host cell, including the collapse of the peripheral ER network and loss of Golgi integrity.


id: cord-272018-txdc0c3j
author: Laing, Eric D.
title: Enhanced Autophagy Contributes to Reduced Viral Infection in Black Flying Fox Cells
date: 2019-03-14
extension: .txt
mime: text/plain
words: 6911
sentence: 241
flesch: 41
cache: cache/cord-272018-txdc0c3j.txt
txt: txt/cord-272018-txdc0c3j.txt
summary:
  Although we cannot yet definitively associate autophagy function with host tolerance to ABLV infection in black flying fox cells, ongoing experiments will refine our mechanistic understanding of enhanced basal autophagy and virus-induced autophagy in bat cells, and further characterize the pro-survival role of autophagy during virus infection. Enhancement of basal and virus-induced autophagy in bat cells connects related reports that long-lived species possess homeostatic processes that dampen oxidative stress and macromolecule damage.


id: cord-001270-l8aa9cl3
author: Wongsrikeao, Pimprapar
title: Antiviral restriction factor transgenesis in the domestic cat
date: 2011-09-11
extension: .txt
mime: text/plain
words: 6277
sentence: 246
flesch: 41
cache: cache/cord-001270-l8aa9cl3.txt
txt: txt/cord-001270-l8aa9cl3.txt
summary:
  To investigate whether more than one transgene could be expressed in cat embryos in a single step by PVSMI, we microinjected 418 oocytes with single-or dual-transgene lentiviral vectors. A practical capability for cat transgenesis is needed to realize the distinctive potential of research on this neurobehaviorally complex, accessible species for advancing human and feline health.


id: cord-319842-4mnaicki
author: Jackson, William T
title: Subversion of Cellular Autophagosomal Machinery by RNA Viruses
date: 2005-04-26
extension: .txt
mime: text/plain
words: 6959
sentence: 250
flesch: 33
cache: cache/cord-319842-4mnaicki.txt
txt: txt/cord-319842-4mnaicki.txt
summary:
  As can be seen in Figure 1 , the punctate GFP-LC3 signal colocalized with that of poliovirus RNA replication protein 3A, as visualized by immunofluorescence. It is thought that one function for membrane localization of viral RNA replication proteins is to promote their oligomerization [6, 7] .


id: cord-346554-a98pjtxs
author: Uddin, Md Bashir
title: Inhibitory effects of bee venom and its components against viruses in vitro and in vivo
date: 2016-11-26
extension: .txt
mime: text/plain
words: 7918
sentence: 286
flesch: 48
cache: cache/cord-346554-a98pjtxs.txt
txt: txt/cord-346554-a98pjtxs.txt
summary:
  BV or MLT antiviral activity was tested in HeLa cells with H3-GFP and EV-71 viruses using standard antiviral activity assays. Additionally, BV or MLT antiviral activity for EV-71 was determined by evaluating 50% tissue culture infective dose (TCID 50 ) and analysis of mRNA expression levels of the EV-71 VP1 gene.


id: cord-103868-iwpiti2h
author: Harrison, Angela R.
title: The Ebola virus interferon antagonist VP24 undergoes active nucleocytoplasmic trafficking
date: 2020-08-11
extension: .txt
mime: text/plain
words: 2265
sentence: 80
flesch: 35
cache: cache/cord-103868-iwpiti2h.txt
txt: txt/cord-103868-iwpiti2h.txt
summary:
  Nuclear export is not required for STAT1 antagonism, consistent with competitive karyopherin binding being the principal antagonistic mechanism while export mediates return of nuclear VP24 to the cytoplasm for replication functions. Nuclear export is not 27 required for STAT1 antagonism, consistent with competitive karyopherin binding being the 28 principal antagonistic mechanism while export mediates return of nuclear VP24 to the 29 cytoplasm for replication functions.


id: cord-003705-ekhj8ae8
author: Azkanaz, Maria
date: 2019-06-14
extension: .txt
mime: text/plain
words: 9392
sentence: 333
flesch: 40
cache: cache/cord-003705-ekhj8ae8.txt
txt: txt/cord-003705-ekhj8ae8.txt
title:
  Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock

summary:
  Intra-nucleolar levels of H3K27me3 and H2AK119ub were not increased in heat shocked cells versus untreated cells, suggesting that PcG proteins are not involved in Polycomb-mediated silencing of nucleolar chromatin ( Figure 3E ). Whether it is unfolding of nucleolar proteins that cause retention of PcG proteins or actually partial unfolding of PcG proteins (or both) remains to be elucidated.


id: cord-299509-7xjdryoq
author: Scholte, Florine E. M.
date: 2013-08-01
extension: .txt
mime: text/plain
words: 9731
sentence: 358
flesch: 44
cache: cache/cord-299509-7xjdryoq.txt
txt: txt/cord-299509-7xjdryoq.txt
title:
  Characterization of Synthetic Chikungunya Viruses Based on the Consensus Sequence of Recent E1-226V Isolates

summary:
  To examine CHIKV-induced translational shut-off, the synthesis of 35 S-labeled viral and cellular proteins during the course of CHIKV LS3 infection was analyzed by metabolic labeling of infected 293/ACE2 cells with 35 S-Met and 35 S-Cys (Fig. 3C) . CHIKV RNA was isolated from virions using the QIAamp Viral RNA mini kit.


id: cord-253709-frauasts
author: Lan, Dongming
date: 2011-08-15
extension: .txt
mime: text/plain
words: 2067
sentence: 84
flesch: 46
cache: cache/cord-253709-frauasts.txt
txt: txt/cord-253709-frauasts.txt
title:
  An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

summary:
  Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography.


id: cord-316814-9fv9xrln
author: Li, Hong-Ye
title: Use of GFP to Investigate Expression of Plant-Derived Vaccines
date: 2009
extension: .txt
mime: text/plain
words: 2822
sentence: 151
flesch: 49
cache: cache/cord-316814-9fv9xrln.txt
txt: txt/cord-316814-9fv9xrln.txt
summary:
  Results of western blot analysis using antibodies against GFP of total plant proteins extracted from tobacco leaves infiltrated with plasmid pCV12 (expressing the S1:GFP fusion) are shown in Fig. The GFP tag enables a relatively quick confirmation of antigen expression in plant cells by fluorescent microscopy.


id: cord-000933-nn9gj0z1
author: Krzyzaniak, Magdalena Anna
date: 2013-04-11
extension: .txt
mime: text/plain
words: 10362
sentence: 470
flesch: 50
cache: cache/cord-000933-nn9gj0z1.txt
txt: txt/cord-000933-nn9gj0z1.txt
title:
  Host Cell Entry of Respiratory Syncytial Virus Involves Macropinocytosis Followed by Proteolytic Activation of the F Protein

summary:
  Thus, we concluded that infectious RSV cell entry and endocytosis were associated with activation of EGFR and its downstream signaling partners including PI3K and PKC. It is noteworthy that iressa was only inhibitory when present during the first hour of virus cell contact confirming that its effect was entry-specific (data not shown).


id: cord-280454-etf32afd
author: Moustaqil, Mehdi
date: 2020-06-05
extension: .txt
mime: text/plain
words: 10177
sentence: 429
flesch: 51
cache: cache/cord-280454-etf32afd.txt
txt: txt/cord-280454-etf32afd.txt
title:
  SARS-CoV-2 proteases cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species and the search for reservoir hosts

summary:
  (*) the protein sequences for Cotton rats (Sigmodon hispidus) and Minks (Neovison vison) were found using tblastn against shotgun genomes, with the Query AAH7172.1 for IRF3 protein [(Isoform 1) Homo sapiens] and NP_653288.1 for NACHT, LRR and PYD domains-containing protein 12 (C) Schematic representation of TAB1 protein structure with the location of the identified cleavage sites on the primary sequence.


id: cord-275307-d7htyfcl
author: Gaglia, Marta Maria
date: 2015-12-08
extension: .txt
mime: text/plain
words: 10873
sentence: 450
flesch: 53
cache: cache/cord-275307-d7htyfcl.txt
txt: txt/cord-275307-d7htyfcl.txt
title:
  Transcriptome-Wide Cleavage Site Mapping on Cellular mRNAs Reveals Features Underlying Sequence-Specific Cleavage by the Viral Ribonuclease SOX

summary:
  In this study, we address this paradox by using high-throughput sequencing of cleavage intermediates combined with a custom bioinformatics-based analysis pipeline to identify SOX cleavage sites across the mRNA transcriptome. To dissect the specificity of SOX cleavage across the mRNA transcriptome, we designed an approach to map SOX cleavage sites in endogenous mRNAs using PARE


id: cord-315483-l6dm82pp
author: Santhakumar, Diwakar
date: 2018-05-01
extension: .txt
mime: text/plain
words: 10827
sentence: 401
flesch: 39
cache: cache/cord-315483-l6dm82pp.txt
txt: txt/cord-315483-l6dm82pp.txt
title:
  Chicken Interferon-induced Protein with Tetratricopeptide Repeats 5 Antagonizes Replication of RNA Viruses

summary:
  The second exon codes the rest of the ORF (1435 bps) and 3′ untranslated regions (UTR) for chicken IFIT5 gene. Chicken cells were transfected with 500 ng of mammalian expression vectors encoding V5-tagged chIFIT5 for 24 hours and were left untreated (NDV-) or were treated with 1 MOI of NDV-GFP (NDV+) for another 24 hours before fixation, staining for nucleus (blue), chIFIT5 (red) and GFP marker (NDV).


id: cord-342189-ya05m58o
author: Banerjee, Abhik K.
title: SARS-CoV-2 disrupts splicing, translation, and protein trafficking to suppress host defenses
date: 2020-10-08
extension: .txt
mime: text/plain
words: 11499
sentence: 406
flesch: 45
cache: cache/cord-342189-ya05m58o.txt
txt: txt/cord-342189-ya05m58o.txt
summary:
  Because viral membrane proteins also require trafficking to the ER, viral disruption of SRP might negatively impact viral propagation, unless viral proteins are trafficked in an SRP-independent manner ( Figure S7E ) or if NSP8/9 selectively impacts host (but not viral) proteins. Understanding the interactions between viral proteins and components of human cells is essential for elucidating their pathogenic mechanisms and for development of effective therapeutics.


id: cord-002076-7t4d4vvo
author: Li, Yongfeng
date: 2016-05-06
extension: .txt
mime: text/plain
words: 5001
sentence: 191
flesch: 31
cache: cache/cord-002076-7t4d4vvo.txt
txt: txt/cord-002076-7t4d4vvo.txt
title:
  Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology

summary:
  Interestingly, reporter viruses in combination with other approaches, such as RNAi, have been applied to screen anti-CSFV ISGs [15] , which is time-and cost-effective. Interestingly, the Cre recombinase as a reporter is used to generate RCREVs for visualizing virus infection in engineered cell lines or transgenic animals harboring a loxP-flanked fluorescent marker upstream of another otherwise silenced fluorescent reporter


id: cord-348799-qu4zin3o
author: Wu, Nannan
date: 2019-10-10
extension: .txt
mime: text/plain
words: 11434
sentence: 412
flesch: 42
cache: cache/cord-348799-qu4zin3o.txt
txt: txt/cord-348799-qu4zin3o.txt
title:
  The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation

summary:
  B) Control cells were analyzed by confocal microscopy with the endogenous P bodies markers TNRC6A and DDX6 (ISG20-expressing cells and a portion of the overlay of control cells depicted here are presented in Fig 5A) . Translationcompetent mRNA generated in vitro were transfected in ISG20 expressing cells, prior to cell lysis one hour afterwards and analyses by RT-qPCR or luciferase assays.


id: cord-307067-cpc1yefj
author: van Doremalen, Neeltje
title: A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques
date: 2020-06-10
extension: .txt
mime: text/plain
words: 6256
sentence: 253
flesch: 45
cache: cache/cord-307067-cpc1yefj.txt
txt: txt/cord-307067-cpc1yefj.txt
summary:
  To investigate whether vaccination with ChAdOx1 MERS vaccination provides protection against a variety of different MERS-CoV strains, we vaccinated BALB/c mice transgenic for hDPP4 with ChAdOx1 MERS or ChAdOx1 GFP 28 days before challenge with 10 4 TCID 50 of one of six diverse MERS-CoV strains ( fig. In the current manuscript, we show that a single dose of ChAdOx1 MERS vaccine protects rhesus macaque model against a mucosal challenge with HCoV-EMC/2012.


id: cord-102184-8u73adnk
author: Li, Jinzhi
title: Visual input into the Drosophila melanogaster mushroom body
date: 2020-05-02
extension: .txt
mime: text/plain
words: 8719
sentence: 280
flesch: 47
cache: cache/cord-102184-8u73adnk.txt
txt: txt/cord-102184-8u73adnk.txt
summary:
  We have a thorough understanding of how olfactory Kenyon cells integrate input from the 470 antennal lobe: most Kenyon cells receive, on average, input from seven projection neurons and the projection neurons connecting to the same Kenyon cell share no apparent common features (Caron et al., 2013; One to three-day-old flies were used when mapping the connectivity rate between input neurons 570 and α/βp Kenyon cells.


id: cord-330176-1ugzkf22
author: Weeratunga, Prasanna
date: 2016-01-05
extension: .txt
mime: text/plain
words: 7988
sentence: 281
flesch: 45
cache: cache/cord-330176-1ugzkf22.txt
txt: txt/cord-330176-1ugzkf22.txt
title:
  RETRACTED ARTICLE: Interferon-mediated antiviral activities of Angelica tenuissima Nakai and its active components

summary:
  These results indicate that oral inoculation of Angelica tenuissima Nakai extracts stimulate mucosal immune cells of small intestine and ultimately induce the strong inhibition of influenza virus replication in bronchoalveolar through the mucosal immune response system. Of 11 fractions, FR-5 was detected at a wavelength and retention time of 325 nm and 16.33 min, and the concentration of FR-5 in 6.0 mg/ml (w/v) of Angelica tenuissima Nakai extract was found to be 5.48 μg/ml ( Fig. 7A and B) .


id: cord-001365-6u80p5sj
author: Weger-Lucarelli, James
title: A Novel MVA Vectored Chikungunya Virus Vaccine Elicits Protective Immunity in Mice
date: 2014-07-24
extension: .txt
mime: text/plain
words: 8483
sentence: 342
flesch: 46
cache: cache/cord-001365-6u80p5sj.txt
txt: txt/cord-001365-6u80p5sj.txt
summary:
  For the production of recombinant MVA vaccines, the CHIKV E2 and E3 genes (hereafter called p62) were PCR amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and cloned into a specially modified poxvirus transfer vector, pI2-Red [37] . Green represents CHIKV E2 positive cells, red is dsRed protein produced by the virus and blue is nuclear staining with Hoechst.


id: cord-319517-denczc6t
author: Salipalli, Sandeep
title: Recent advances in live cell imaging of hepatoma cells
date: 2014-07-08
extension: .txt
mime: text/plain
words: 9219
sentence: 347
flesch: 41
cache: cache/cord-319517-denczc6t.txt
txt: txt/cord-319517-denczc6t.txt
summary:
  An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore Evolutionary optimization of fluorescent proteins for intracellular FRET Fluorescent proteins from nonbioluminescent Anthozoa species A monomeric red fluorescent protein Dynamic motion of paxillin on actin filaments in living endothelial cells A guide to choosing fluorescent proteins Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein Bright monomeric red fluorescent protein with an extended fluorescence lifetime Green fluorescent protein as a marker for gene expression Aequorea green fluorescent protein: expression of the gene and fluorescence characteristics of the recombinant protein Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging In vitro activity of fluorescent dyes against asexual blood stages of Plasmodium falciparum Antibodies for fluorescent molecular rotors ReAsH/FlAsH labeling and image analysis of tetracysteine sensor proteins in cells Specific covalent labeling of recombinant protein molecules inside live cells Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications Fluorogen-activating single-chain antibodies for imaging cell surface proteins Moreover, ELuc was tested in cell lines such as NIH/3T3 using various promoters, and it was shown that the luciferase primarily localizes in nucleus, cytosol and in peroxisomes.


id: cord-023026-2r84ndzv
title: Posters
date: 2013-06-14
extension: .txt
mime: text/plain
words: 138804
sentence: 5842
flesch: 38
cache: cache/cord-023026-2r84ndzv.txt
txt: txt/cord-023026-2r84ndzv.txt
summary:
  Levels of expression of NG2 mRNA and protein in mouse glial cells are regulated by the neurotransmitter glutamate and overexpression of cleaved fragments modulates protein expression in murine glial cells. Interestingly, when we analyzed the in vitro expression pattern of rCD300f in brain cells by Q-PCR and immunohistochemistry, in addition to the expected expression in microglial cells, we detected expression of CD300f in oligodendrocytes and neurons.


id: cord-004534-jqm1hxps
title: Abstract
date: 2009-06-09
extension: .txt
mime: text/plain
words: 139178
sentence: 5595
flesch: 39
cache: cache/cord-004534-jqm1hxps.txt
txt: txt/cord-004534-jqm1hxps.txt
summary:
  This simple model illustrates how differential detergent selectivity for faces and strong constraints coming from purely environmental features could influence transmembrane helix packing, membrane protein structure and assembly. Imaging of mobile stable lipid rafts in the live cell plasma membrane M. Brameshuber 1 , J. Weghuber 1 , V. Ruprecht 1 , H. Stockinger 2 , G. J. Schuetz 1 1 Johannes Kepler University Linz, Austria, 2 Medical University of Vienna, Austria The organization of the cellular plasma membrane at a nanoscopic length scale is believed to affect the association of distinct sets of membrane proteins for the regulation of multiple signaling pathways.


id: cord-023211-kt5gt26t
title: Poster Session Abstracts
date: 2007-08-29
extension: .txt
mime: text/plain
words: 221712
sentence: 9857
flesch: 48
cache: cache/cord-023211-kt5gt26t.txt
txt: txt/cord-023211-kt5gt26t.txt
summary:
  Understanding how its modulation modifies transepithelial transport and inflammation in CF versus non CF cells should give new therapeutic clues to reduce exaggerated inflammation and improve fluid secretion in CF patients. This finding indicates that CFTR potentiators have a wide efficacy on many class III mutants and therefore may represent a promising therapeutic strategy for a significant number of CF patients.


id: cord-257167-rz4r5sj7
title: Abstracts for the 29th Annual Meeting of the Japan Neuroscience Society (Neuroscience2006)
date: 2006-12-31
extension: .txt
mime: text/plain
words: 241480
sentence: 10903
flesch: 43
cache: cache/cord-257167-rz4r5sj7.txt
txt: txt/cord-257167-rz4r5sj7.txt
summary:
  Inst., Kyoto Univ., Inuyama, Japan GABAergic cells in the cerebral cortex are divided into subgroups: parvalbumin (PV)-, somatostatin (SOM)-, calretinin (CR)-, and calbindin-containing types. In the present study, we therefore investigated the synthesis of sex steroids and corticosteroids in the hippocampus of adult male rats, by monitoring the metabolism of tritiated steroids with HPLC system. PS2A-G125 GABA depolarizes GnRH neurons isolated from adult GnRH-EGFP transgenic rats Chengzhu Yin, Nobuyuki Tanaka, Masakatsu Kato, Yasuo Sakuma Nippon Medical School, Department of physiology, Tokyo, Japan GnRH neurons are essential in the reproductive neuroendocrine system.


# ent

id: cord-018771-xqlb74px
sid: 4
eid: 2
type: ORG
entity:
  nach der anforderung aus der blutbank treffen die bestellten erythrozytenkonzentrate ein – es sind erythrozytenkonzentrate der blutgruppe a. sie sind eindeutig auf den namen des patienten ausgezeichnet


id: cord-018771-xqlb74px
sid: 7
eid: 2
type: ORG
entity:
  nach der anforderung aus der blutbank treffen die bestellten erythrozytenkonzentrate ein -es sind erythrozytenkonzentrate der blutgruppe a. sie sind eindeutig auf den namen des patienten ausgezeichnet


id: cord-018771-xqlb74px
sid: 19
eid: 6
type: PERSON
entity:
  erschienenen richtlinien zur gewinnung von blut und blutbestandteilen und zur anwendung von blutprodukten


id: cord-018771-xqlb74px
sid: 31
eid: 1
type: PERSON
entity:
  seit der generellen einführung von leukozytendepletierten ek gibt es nur noch wenige indikationen für gewaschene ek


id: cord-018771-xqlb74px
sid: 35
eid: 2
type: ORG
entity:
  anämie den verlauf einer erkrankung negativ beeinflussen und führt zu keiner besseren toleranz bei weiteren hämoglobinabfällen.


id: cord-018771-xqlb74px
sid: 79
eid: 8
type: PERSON
entity:
  nach der transfusion eine serologische untersuchung zur feststellung von antikörpern gegen den rhesusfaktor erfolgen


