item: #1 of 41
          id: cord-000933-nn9gj0z1
      author: Krzyzaniak, Magdalena Anna
       title: Host Cell Entry of Respiratory Syncytial Virus Involves Macropinocytosis Followed by Proteolytic Activation of the F Protein
        date: 2013-04-11
       words: 10362
      flesch: 50
     summary: Thus, we concluded that infectious RSV cell entry and endocytosis were associated with activation of EGFR and its downstream signaling partners including PI3K and PKC. It is noteworthy that iressa was only inhibitory when present during the first hour of virus cell contact confirming that its effect was entry-specific (data not shown).
    keywords: 37uc; 4uc; a549; actin; activation; addition; af488; analysis; anti; antibody; assay; associated; binding; blots; buffer; cdc42; cells; cleavage; cme; cmk; cold; concentration; confocal; control; data; dec; dioc; dmem; effect; egfr; eipa; endocytic; endocytosis; entry; epithelial; experiments; expression; facs; factors; fig; fluorescence; formaldehyde; furin; fusion; gfp; goat; green; hela; hela cells; hepes; human; ice; infected; infection; inhibited; inhibition; inhibitors; inoculum; internalization; intracellular; kda; macropinocytosis; macropinosomes; maturation; medium; membrane; microscopy; min; mock; moi; myosin; page; pak1; particles; pbs; peptide; positive; present; processing; protein; proteolytic; purified; r18; rab5; rab7; red; respiratory; results; role; rsv; rsv infection; rvkr; samples; sds; second; serum; sfv; software; spots; srm; syncytial; time; trypsin; uptake; vacuoles; viral; virus; viruses; vlps; western
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        item: #2 of 41
          id: cord-001123-n2e4s7bu
      author: Lin, Yue-Zhi
       title: The Soluble Form of the EIAV Receptor Encoded by an Alternative Splicing Variant Inhibits EIAV Infection of Target Cells
        date: 2013-11-22
       words: 5900
      flesch: 45
     summary: Therefore, the present study had three objectives: 1) to identify alternative splicing variants for ELR1, 2) to determine whether any of these variants encode soluble ELR1 and 3) to characterize the roles of any soluble forms of the receptor in EIAV infection. Horses and related experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee (IACUC) of the Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences. The expression of soluble ELR1 (sELR1) was examined by Western blot, flow cytometry and confocal microscopy.
    keywords: activity; addition; alternative; anemia; antibody; assay; binding; blot; cdna; cells; cellular; confocal; control; culture; data; different; dlv34; domain; effect; eiav; eiav infection; elr1; emdms; equine; expression; factor; fed; fig; form; fragment; functional; gfp; horses; host; human; imaging; important; infection; intron; levels; live; major; medium; membrane; min; mrna; pcdna3.1; pcr; protein; receptor; recombinant; reverse; soluble; species; splicing; strain; studies; study; t cells; target; target cells; time; transfection; transmembrane; usa; variants; vector; viral; virus; viruses; western
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        item: #3 of 41
          id: cord-001270-l8aa9cl3
      author: Wongsrikeao, Pimprapar
       title: Antiviral restriction factor transgenesis in the domestic cat
        date: 2011-09-11
       words: 6277
      flesch: 41
     summary: To investigate whether more than one transgene could be expressed in cat embryos in a single step by PVSMI, we microinjected 418 oocytes with single-or dual-transgene lentiviral vectors. A practical capability for cat transgenesis is needed to realize the distinctive potential of research on this neurobehaviorally complex, accessible species for advancing human and feline health.
    keywords: aids; analysis; animal; anti; antibody; blastocysts; blot; bsa; cat; cats; cell; control; cortex; cultured; day; development; disease; dna; domestic; dual; embryo; example; expression; factor; fallopian; feline; fertilization; fig; fiv; fluorescent; genome; genomic; gentamicin; germline; gfp; green; high; human; immunodeficiency; injection; ivf; kittens; lack; lentiviral; lentiviruses; level; light; macaque; medium; mice; microinjection; min; models; mosaicism; mouse; non; oocytes; organs; p.w; pbmcs; pcr; positive; potential; primer; promoter; proteins; rates; replication; restriction; results; rhesus; rhtrimcyp; roche; single; size; southern; species; specific; sperm; supplementary; supplementary fig; susceptible; table; temperature; term; tgcat1; therapy; time; tissue; transduction; transfer; transgenesis; transgenic; transmission; tube; vector; virus; vitro; world; zygotes; ° c
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        item: #4 of 41
          id: cord-001365-6u80p5sj
      author: Weger-Lucarelli, James
       title: A Novel MVA Vectored Chikungunya Virus Vaccine Elicits Protective Immunity in Mice
        date: 2014-07-24
       words: 8483
      flesch: 46
     summary: For the production of recombinant MVA vaccines, the CHIKV E2 and E3 genes (hereafter called p62) were PCR amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and cloned into a specially modified poxvirus transfer vector, pI2-Red [37] . Green represents CHIKV E2 positive cells, red is dsRed protein produced by the virus and blue is nuclear staining with Hoechst.
    keywords: a129; addition; analysis; ankara; antibodies; antibody; attenuated; balb; boost; buffer; candidate; cd4; cd8; cells; challenge; chik; chikungunya; contrast; control; data; days; deficient; depleted; depletion; dilution; disease; dna; effective; encephalitis; equine; expression; fig; following; footpad; gfp; high; immune; immunity; infected; infection; interferon; ires; lethal; levels; live; mice; min; model; modified; mortality; mouse; mva; neutralizing; novel; outbreaks; p62; pcr; peptide; plates; pools; positive; post; presence; prime; protection; protein; recent; recombinant; report; response; results; role; samples; serum; signaling; specific; strain; studies; study; swelling; t cells; tcid; transfer; type; vaccinated; vaccination; vaccine; vaccinia; vector; vectored; venezuelan; viral; viremia; virus; viruses; wild
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        item: #5 of 41
          id: cord-002076-7t4d4vvo
      author: Li, Yongfeng
       title: Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology
        date: 2016-05-06
       words: 5001
      flesch: 31
     summary: Interestingly, reporter viruses in combination with other approaches, such as RNAi, have been applied to screen anti-CSFV ISGs [15] , which is time-and cost-effective. Interestingly, the Cre recombinase as a reporter is used to generate RCREVs for visualizing virus infection in engineered cell lines or transgenic animals harboring a loxP-flanked fluorescent marker upstream of another otherwise silenced fluorescent reporter
    keywords: addition; antiviral; applications; applied; assays; cdna; cells; cellular; classical; competent; csfv; development; different; disease; egfp; example; expression; fever; fluc; fluorescent; gene; generation; genetic; genome; gfp; green; hepatitis; high; host; hts; human; imaging; infected; infection; influenza; insertion; interactions; labeling; life; living; luciferase; marker; novel; polymerase; powerful; promising; protein; rapid; rcrevs; real; receptor; recombinant; red; replication; reporter; rluc; rna; screening; small; split; swine; tests; throughput; time; tools; tracking; type; viral; virology; virus; viruses; vivo; wild
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        item: #6 of 41
          id: cord-002320-m99amd4y
      author: Mathur, Kalika
       title: Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity
        date: 2016-11-30
       words: 5683
      flesch: 48
     summary: Taken together, the current study has identified CHIKV proteins nsP2 and nsP3 to exhibit RNAi suppressor activity in the in-vitro system that was further demonstrated in its natural hosts, namely, an Aedes and mammalian cell line. RNA-based antiviral immunity Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants RNAi function, diversity, and loss in the fungal kingdom Mosquito RNAi is the major innate immune pathway controlling arbovirus infection and transmission Dicing of viral replication intermediates during silencing of latent Drosophila viruses Small silencing RNAs: an expanding universe The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster The rest is silence Dicing and slicing: the core machinery of the RNA interference pathway
    keywords: activity; aedes; alphaviruses; analysis; antiviral; assay; binding; cell; chikv; complex; conserved; control; different; domain; dsrna; exhibit; expression; facs; fhvb2; fig; genes; gfp; gfpshrna; helicase; host; infection; infiltration; interference; leaves; line; mammalian; motifs; mutants; natural; negative; non; nsp2; nsp3; nsp4; pathway; pib; plasmid; positive; possess; presence; protease; proteins; replication; reversal; reversion; risc; rnai; rnai suppressor; role; sensor; sf21; significant; silencing; sirna; small; species; specific; structural; study; suppressor; suppressor activity; system; test; transfection; value; vector; viral; virus; vsrs
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        item: #7 of 41
          id: cord-003705-ekhj8ae8
      author: Azkanaz, Maria
       title: Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock
        date: 2019-06-14
       words: 9392
      flesch: 40
     summary: Intra-nucleolar levels of H3K27me3 and H2AK119ub were not increased in heat shocked cells versus untreated cells, suggesting that PcG proteins are not involved in Polycomb-mediated silencing of nucleolar chromatin ( Figure 3E ). Whether it is unfolding of nucleolar proteins that cause retention of PcG proteins or actually partial unfolding of PcG proteins (or both) remains to be elucidated.
    keywords: accumulation; activity; addition; aging; analysis; anti; associated; bars; binding; blood; buffer; cbx2; cbx4; cbx8; cd34; cells; cellular; center; changes; chaperone; chip; chromatin; complex; control; data; dependent; different; disease; dna; dnajb1; endogenous; epigenetic; error; essential; et al; experiments; expression; ezh2; family; figure; fisher; flip; fraction; frap; genes; genome; gfp; h2ak119ub; h3k27me3; heat; heat shock; hela; histone; hsp70; hsps; human; images; increase; induced; inhibition; k562; k562 cells; k562 gfp; kinetics; landscape; levels; like; line; liquid; localization; loss; machinery; mean; min; modifications; modifiers; nuclear; nucleolar; nucleoli; nucleolus; pcg; pcg proteins; physiological; polycomb; prc1; prc1/2; prc2; proteasome; proteins; prrl; quality; quick; recovery; reduction; regulation; regulators; response; reversible; ribosomal; samples; scientific; sffv; shock; silencing; solid; stem; stress; subunits; supplement; target; thermo; transcription; ubiquitin; untreated; van
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        item: #8 of 41
          id: cord-004534-jqm1hxps
      author: None
       title: Abstract
        date: 2009-06-09
       words: 139178
      flesch: 39
     summary: This simple model illustrates how differential detergent selectivity for faces and strong constraints coming from purely environmental features could influence transmembrane helix packing, membrane protein structure and assembly. Imaging of mobile stable lipid rafts in the live cell plasma membrane M. Brameshuber 1 , J. Weghuber 1 , V. Ruprecht 1 , H. Stockinger 2 , G. J. Schuetz 1 1 Johannes Kepler University Linz, Austria, 2 Medical University of Vienna, Austria The organization of the cellular plasma membrane at a nanoscopic length scale is believed to affect the association of distinct sets of membrane proteins for the regulation of multiple signaling pathways.
    keywords: a. m.; a30p; aba; able; absence; absorption; academy; acceleration; acceptor; accessible; account; accumulation; accuracy; acid; acidic; acquisition; actin; activation; active; activities; activity; acyl; addition; address; adhesion; adp; adult; advanced; advantage; affected; affinity; afm; agents; aggregates; aggregation; agreement; aim; albumin; algorithm; alp14; alpha; altered; alternative; alzheimer; amb; amino; amounts; amplitude; amyloid; amyloidogenic; analogues; analysis; analytical; angle; animals; anionic; anisotropy; antenna; antibiotics; antibody; anticancer; antigen; antimicrobial; apoptosis; apoptotic; apparent; applications; applied; approach; appropriate; aqueous; architecture; area; aromatic; arrays; artificial; aspects; assay; assemblies; assembly; associated; atomic; atomic force; atomistic; atoms; atp; atpase; attached; attachment; attention; attractive; austria; available; average; axis; azurin; backbone; background; bacterial; balance; bands; barcelona; barrier; base; basis; bax; beads; beam; behavior; belgium; berlin; best; beta; better; bianchini; bilayer; binary; binding; biochemical; biochemistry; biofisica; biological; biological membranes; biology; biomedical; biomembrane; biomolecular; biophysics; biosensors; biotechnology; biotin; blood; bodipy; body; bologna; bond; bovine; brain; breast; bridge; brightness; broad; brownian; bsa; budding; buffer; build; caf1; cagliari; calcium; calculated; calculations; calorimetry; cancer; cancer cells; candidate; capable; capsid; carbohydrate; carbon; cardiac; cardiomyocytes; carlo; carriers; case; catalysis; catalytic; cationic; causes; cavities; cea; cell; cell biology; cell cycle; cell imaging; cell membrane; cell surface; cellular; center; central; centre; certain; chain; changes; changing; channel; chaperone; characteristic; characterization; characterized; charge; chemical; chemistry; chip; chitosan; chloride; chlorophyll; chloroplast; cholesterol; chromatin; chromophore; circular; cis; clamp; class; classical; clavulanic; clc; cleavage; clinical; close; clustering; clusters; cnr; cnrs; coarse; coated; coefficient; coil; cold; coli; collagen; collagenase; collective; college; colloids; color; combination; combined; common; compact; comparable; comparison; complementary; complete; complex; complexes; components; composition; compounds; computational; computer; concentration; concept; conclusion; condensation; conditions; conductance; configuration; confined; confinement; confocal; confocal microscopy; conformational; conformational changes; consequence; conserved; considerable; consistent; constant; constructs; contact; content; continuous; contraction; contrast; contribute; contribution; control; controlling; conventional; conversion; coordination; core; correlation; corresponding; counterions; counting; coupling; covalent; cp29; cpt; critical; cross; crucial; crystal; crystalline; crystallography; culture; current; curvature; curves; cycle; cyclic; cysteine; cytochrome; cytometry; cytoplasm; cytoskeleton; cytosolic; damage; dark; data; day; days; death; decay; decrease; decreasing; deeper; defensins; degradation; degree; delivery; demonstrated; denaturation; denmark; densities; density; department; dependent; depletion; deposition; dept; derivatives; description; design; destabilization; detailed; details; detection; detergent; determination; determined; developed; development; device; diabetes; diameter; diaspro; dichroism; differences; different; different lipid; different membrane; different molecular; different proteins; differential; differentiation; diffraction; diffusion; dimensional; dimer; dimerization; dipartimento; dipole; direct; direction; discovery; disease; disordered; displacement; display; dissociation; distance; distinct; distribution; diverse; division; dmpc; dna; dna complexes; dna molecule; docking; domain; donor; dopc; double; dppc; drug; dsc; duplex; duration; dyes; dynamical; dynamics; dynamics simulations; e.g.; early; ecs; effect; effective; efficacy; efficiency; ejection; elastic; elc; electric; electrochemical; electrode; electron; electron microscopy; electrophoresis; electrophysiological; electrostatic; elements; elongation; emission; emodin; endogenous; energies; energy; energy transfer; engineered; engineering; enhancement; enriched; ensemble; enthalpy; entropy; entry; environment; enzymatic; enzyme; epr; equilibrium; erythrocytes; esr; essential; estimate; estimation; etbr; ethanol; eukaryotic; evanescent; events; evidence; example; excess; exchange; excitation; excited; exhibits; existence; expansion; experimental data; experimental results; experiments; explanation; exponential; exposure; expression; extended; extension; extensive; extent; external; extracts; extrusion; factors; faculty; failure; family; far; fast; faster; fatty; fccs; fcs; features; fibers; fibres; fibrillar; fibrils; field; filaments; film; filter; findings; fisica; fit; flexibility; flim; flow; fluctuations; fluid; fluidity; fluorescence; fluorescence correlation; fluorescence emission; fluorescence experiments; fluorescence intensity; fluorescence lifetime; fluorescence microscopy; fluorescence properties; fluorescence techniques; fluorescent protein; fluorophores; flux; fluxes; focus; focused; following; force; force microscopy; force spectroscopy; formation; forms; forward; fourier; fps; fragments; frame; france; frap; free; free energy; frequencies; frequency; fret; ftir; function; functionalized; functioning; fundamental; fungal; future; g protein; gag; gain; gap; gating; gel; gels; gene; general; generation; genetic; genome; genomic; genova; georgia; germany; gfp; giant; glucose; glutamate; glycation; goal; gold; good; gram; great; greater; green; grenoble; group; growth; guvs; göttingen; hairpin; half; hand; harmonic; head; heat; heating; heavy; heidelberg; helical; helices; helix; help; heme; hemoglobin; heparin; hepatitis; heterogeneity; high; higher; histone; hiv-1; hmga2; hmgb1; homologous; hormone; host; hours; hp1; hsa; hsp22; human; hungary; hybrid; hydrated; hydration; hydrodynamic; hydrogels; hydrogen; hydrolysis; hydrophobic; hyp; hypothesis; iapp; ideal; identical; identification; iii; iit; illumination; image; imaging; imaging microscopy; immobilization; immobilized; immune; impact; important; important role; inclusions; incorporated; incorporation; increase; incubation; individual; induced; induction; infection; influence; infrared; inhibition; inhibitors; initial; initiation; inner; inorganic; insertion; inside; insight; institute; instrument; insulin; intact; integral; integrin; integrity; intensity; interaction; interest; interesting; interface; interference; interior; intermediate; intermolecular; internal; interplay; intracellular; intrinsic; investigation; involved; ionic; irradiation; islet; isoforms; isolated; isothermal; isotropic; istituto; italian; italy; itc; japan; johannes; jump; kcl; kcsa; kda; key; kinase; kind; kinetics; knowledge; known; ktp; label; labeling; laboratory; lack; lambs; lapse; large; laser; lateral; law; ldl; leading; leaflet; length; level; lfa-1; lifetime; ligand; ligand binding; light; light scattering; like; like protein; likely; limited; limiting; linear; linked; linking; linz; lipid; lipid bilayer; lipid membranes; lipid vesicles; liposomes; liquid; literature; little; live cells; living; living cells; load; local; localization; lodz; london; long; longer; loop; loss; low; lower; lps; luciferase; lung; luv; lysine; lysozyme; macromolecules; macroscopic; magnetic; magnitude; major; mammalian; manner; marker; mass; material; mathematical; matrix; matter; maturation; maximum; means; measured; measurements; mechanical; mechanism; medical; medicine; medium; melting; membrane; membrane fluorescence; membrane fusion; membrane interaction; membrane microdomains; membrane potential; membrane properties; membrane protein; membrane structure; membrane surface; membrane system; membrane tension; metabolic; metal; method; methodology; mice; micelles; microdomains; microscopy; microtubules; migration; milan; millisecond; mimic; minimum; minor; mitochondria; mixed; mixing; mixtures; mobile; mobility; model; model membrane; model protein; model system; modeling; modifications; modified; modulates; modulation; moiety; molecular; molecular biology; molecular biophysics; molecular dynamics; molecular interactions; molecular level; molecular mechanism; molecule fluorescence; molecule force; molecules; monitoring; monolayers; monomeric; monomers; morphological; morphology; motif; motion; motor; movement; mrna; multiple; muscle; mutagenesis; mutant; mutated; mutations; myosin; nadh; nanoclusters; nanoparticles; nanoscale; national; native; natural; nature; ncp7; necessary; need; negative; nerve; nervous; netherlands; network; neurodegenerative; neuronal; neurons; neuroscience; neurotransmitter; neutral; neutron; new; nmr; noise; non; normal; nottingham; novel; nps; nuclear; nucleation; nucleic; nucleotide; nucleus; number; numerical; numerous; objective; observation; observed; occurrence; oligomerization; oligomers; oligonucleotide; ompf; ones; oocytes; open; opening; opposite; optical; optical microscopy; optical properties; orai1; order; organelles; organic; organisms; organization; organized; organs; orientation; original; osmotic; outer; outer membrane; overall; oxford; oxidation; oxidative; oxidized; oxygen; p2x; p53; packing; padova; pairs; palermo; parallel; parameters; parkinson; partial; particles; particular; partition; partitioning; partners; patch; pathological; pathway; patients; patterns; pcer; pdz; peak; peculiar; peg; peptide; permeability; permeation; peroxidation; peroxide; perpendicular; perturbation; pes; petersburg; pgk; phagocytosis; pharmaceutical; pharmacological; phase; phenomenon; phosphate; phosphatidylcholine; phosphatidylglycerol; phospholipid; photobleaching; photodynamic; photolysis; photon; photosynthetic; physical; physical properties; physics; physiological; physiology; picture; pigment; pisa; place; planar; planck; plant; plaques; plasma membrane; platelet; platforms; play; point; poland; polar; polarization; polarized; polymer; polymeric; polymerization; polypeptide; popc; populations; pore; porins; porous; portugal; positions; positive; possibility; possible; post; potassium; potential; powerful; ppme; precision; predictions; preliminary; prepared; presence; present; present study; presentation; pressure; presynaptic; previous; primary; prion; probability; probe; problem; process; processes; produce; production; products; profile; program; proiapp; project; proliferation; proline; promising; propagation; propensity; properties; property; protein; protein aggregation; protein binding; protein complexes; protein conformational; protein dynamics; protein function; protein interactions; protein molecules; protein stability; protein structure; protein surface; protocol; protonation; prp; psii; psm; pulse; pump; pure; purification; purified; purpose; putative; quantification; quantitative; quantum; quasi; quenching; questions; radical; raman; random; range; rapid; rate; ratios; rats; ray; rbc; reaction; reactive; real; rearrangements; recent; receptor; recognition; recombinant; reconstituted; reconstitution; recordings; recovery; redox; reduced; reflection; refolding; regime; region; regulate; regulation; regulatory; related; relationship; relative; relaxation; release; relevance; relevant; remarkable; removal; repair; replication; report; representative; repressor; republic; research; residues; resistance; resolution; resonance; respect; respiratory; response; responsible; results; reverse; reversible; review; rhodopsin; ribozymes; rich; rigid; rise; rna; role; rotational; russia; saint; salt; sample; sampling; sarcomere; saxs; scale; scanning; scattering; scheme; school; sciences; scientific; sclerosis; screening; second; secondary; section; segment; selective; selectivity; self; sense; sensing; sensitive; sensitivity; sensor; separation; sequence; series; serotonin; sers; serum; set; setup; severe; shape; shear; sheet; shg; shift; short; shorter; shows; shr; signal; signaling; significant; silicon; similar; simulations; simultaneous; single; single cell; single channel; single dna; single molecule; single protein; sirna; site; situ; size; skeletal; slices; sln; slovakia; slow; small; smaller; soce; sodium; soft; solid; soluble; solutions; solvent; source; space; spain; spatial; spatio; species; specific; specific dna; specific protein; specificity; spectra; spectroscopy; spectrum; speed; spherical; sphingomyelin; spindle; spontaneous; spr; square; stability; stabilized; stable; stages; standard; starting; state; state university; statistical; steady; stem cells; step; stimulated; stoichiometry; strain; strand; strategies; strategy; strength; stress; stretching; strong; structural changes; structural properties; structure; studied; study; subject; subsequent; substrate; subunit; sugar; suitable; superfamily; support; supramolecular; surface; surfactant; survival; sweden; switching; switzerland; syn; synapses; synaptic; synchrotron; syndrome; synko; synthesis; synthetic; synuclein; system; target; targeted; targeting; tau; tbilisi; techniques; technologies; technology; temperature; temporal; tension; terminal; terminus; terms; test; tethered; theoretical; theory; therapeutic; therapy; thermal; thermodynamic; thickness; thin; threshold; tht; time; tirf; tissue; titin; titration; tocopherol; tool; topology; total; toxic; toxicity; tracking; trafficking; trajectories; transcription; transduction; transfer; transient; transition; translational; translocation; transmembrane; transmission; transport; treatment; trieste; triplet; trp; tryptophan; tumor; turnover; tweezers; type; typical; tyrosine; u.k; u.s.a; ucp4; ukraine; ultrasound; umr; unclear; underlying; understanding; understood; unfolded; unfolding; unilamellar; unique; university; unknown; unusual; unwinding; uptake; usa; use; useful; uses; values; variable; variants; variation; variety; vectors; velocity; venom; versus; vesicles; vgsc; viability; vibrational; view; viral; virus; viruses; viscosity; visible; vitro; vivo; voltage; volume; walls; warwick; water; wavelength; waves; ways; weak; wide; wild; work; working; years; yeast; yidc; zwitterionic; zürich
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        item: #9 of 41
          id: cord-018771-xqlb74px
      author: Turinsky, Sebastian
       title: Bluttransfusion
        date: 2013-04-04
       words: 1808
      flesch: 41
     summary: Was muss der Intensivarzt nun tun? Die Gabe von Blut wurde erstmals erfolgreich im 17. Auch ein zweiter Bedside-Test zur Kontrolle zeigt erneut die Blutgruppe B. Da die Blutung klinisch fortschreitet und der Patient in den OP muss, entschließt sich der Intensivarzt zur Transfusion von Erythrozytenkonzentraten der Blutgruppe 0
    keywords: aktivität; als; antikörpern; anwendung; anämie; auch; auf; auftreten; aus; bedingt; bedside; bei; bis; blutgruppe; blutprodukten; bluttransfusion; das; der; des; die; durch; durchgeführt; eine; erneut; erythrozytenkonzentrate; etwa; führen; für; gabe; gegen; gerinnungsfaktoren; gfp; hämatokrit; innerhalb; intensivarzt; ist; kann; können; mit; muss; nach; negativ; nicht; nur; oder; patienten; rahmen; rhesus; sehr; sich; sie; sind; sollte; sowie; test; therapie; thrombozyten; transfundiert; transfusion; und; vollblut; von; vor; wenige; werden; wie; wird; wurde; zeigt; zur; zwischen; über
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        item: #10 of 41
          id: cord-023026-2r84ndzv
      author: None
       title: Posters
        date: 2013-06-14
       words: 138804
      flesch: 38
     summary: Levels of expression of NG2 mRNA and protein in mouse glial cells are regulated by the neurotransmitter glutamate and overexpression of cleaved fragments modulates protein expression in murine glial cells. Interestingly, when we analyzed the in vitro expression pattern of rCD300f in brain cells by Q-PCR and immunohistochemistry, in addition to the expected expression in microglial cells, we detected expression of CD300f in oligodendrocytes and neurons.
    keywords: -atpase; 24h; 3xtg; abcd1; ability; ablation; able; absence; abundant; accelerated; accumulation; acid; acidic; action; activated; activation; activators; activities; activity; acts; acute; acute brain; ad mice; addition; address; adenosine; adhesion; adjacent; administration; adult; adult brain; adult mice; adulthood; affected; affinity; aged; aged mice; ageing; agent; aggregates; aggregation; agonists; aim; akt; ald; alpha; alterations; altered; alternative; alzheimer; amounts; amplitude; amyloid; analysis; animals; antagonist; anti; antibodies; antibody; antigen; ao cells; apelin; apod; apoptosis; apoptotic; apparent; application; approach; aqp4; areas; artery; aspartate; aspects; assay; associated; association; astrocyte activation; astrocytes; astroglial; astroglial cells; astrogliosis; atp; attenuated; autoimmune; axonal; axons; background; barrier; basal; baseline; basis; bbb; bdnf; behavior; beneficial; bergmann; beta; better; bicarbonate; binding; biological; biology; blocking; blood; blot; body; bone; brain; brain barrier; brain cells; brain development; brain expression; brain function; brain injury; brain microglia; brain regions; brain slices; brain tissue; brainstem; c57bl/6; ca1; ca21; calcium; callosum; candidate; capable; capacity; cascade; cases; catenin; cause; cci; ccl2; cd11b; cd14; cd200r1; cd300f; cd45; cd68; cdk2; cell activation; cell adhesion; cell cultures; cell cycle; cell death; cell differentiation; cell lines; cell population; cell proliferation; cell surface; cell survival; cell types; cells; central; central nervous; cerebellar; cerebral; challenge; changes; channels; characteristics; characterization; characterized; chemokines; cholinergic; chronic; cis; clamp; classical; clearance; clinical; cns; cns cells; cns injury; cognitive; collagen; combination; common; communication; comparison; complex; complexes; components; concentration; conclusions; conditions; confocal; connexin; consequences; consistent; contact; context; contralateral; contrary; contrast; contribution; control; control cells; control mice; controlling; cord; cord cells; core; corpus; correlates; correlation; corresponding; cortex; cortical; cortical astrocytes; coupling; course; coverage; cpz; creb; creert2; critical; cross; crucial; cryab; csf; cultured astrocytes; cultured microglia; cultures; cuprizone; curcumin; current; cx30; cx32; cx3cr1; cx43; cx47; cxcl12; cycle; cytokines; cytometry; cytoplasmic; cytoskeleton; d2r; daily; damage; data; days; death; debris; decline; decreased; defects; deficient; deficient mice; deficits; degeneration; degradation; deletion; delivery; demyelinated; demyelinating; dendritic cells; densities; density; dentate; dependent; deposits; deprivation; derived; detailed; detectable; detrimental; development; diet; differences; different; different brain; different cell; differential; differentiation; diffusion; diminished; direct; disease; disorders; display; disruption; distal; distinct; distribution; diverse; dna; domain; dopa; dorsal; dose; double; dpakkr; dpi; drg; drosophila; drug; dsred; dye; dynamics; dysfunction; e.g.; eae; early; ebpb; ebpd; ecm; effect; effective; efficacy; efficient; egfp; electrical; electron; electrophysiological; elevated; embryonic; embryos; encephalomyelitis; encoding; endogenous; endothelial cells; energy; enhanced; environment; enzyme; ependymal cells; epilepsy; epileptic; erk; erk1/2; essential; events; evidence; evoked; examined; excitatory; excitotoxicity; exhibit; exosomes; expected; experiments; explants; exposed; exposure; expressed; expression; expression analysis; expression levels; extensive; extent; extracellular; eyes; facs; factors; failure; family; fast; fate; fatty; features; fibers; fibronectin; field; findings; flow; fluorescent; focus; fold; forebrain; formation; fraction; free; frequency; function; functional role; future; g93a; gaba; gabaergic; gain; ganglia; ganglion; gap; gbm; gdnf; gene; gene expression; general; generate; genetic; germany; gfap; gfap mice; gfp; glast; glaz; glial activation; glial cells; glial cultures; glioblastoma; glioma cells; global; glt-1; glucose; glutamate; glutamatergic; glutamine; glycogen; graft; group; growth; gsc; gscs; gyrus; hand; healthy; help; hemichannel; heterogeneity; high; higher; hippocampal; histological; homeostasis; hormone; host; hours; hrs; hspb5; hsvtk; human; human astrocytes; human brain; human microglia; hypertension; hypertrophy; hypothalamus; hypothesis; hypoxia; i.p; iba-1; iba1; identification; ifn; igg; iii; il-1b; il-1ra; il-33; il-6; il6; images; imaging; immature; immune cells; immunofluorescence; immunohistochemistry; immunological; immunoreactivity; immunostaining; impact; impaired; impairment; important; important role; improve; increase; incubated; incubation; individual; induced; inducible; induction; infiltration; inflammasome; inflammation; inflammatory; inflammatory activation; inflammatory cells; inflammatory response; influence; influx; inhibited; inhibition; inhibitory; inhibits; initial; initiation; injection; injured; injuries; injury; innate; inner; insight; intact; integrity; intensity; interaction; interest; interferon; interneurons; intrinsic; invasion; investigation; involved; involvement; ips cells; ischemic; isoforms; isolated; jun; key; kinase; kir4.1; knock; knockdown; knockout; knockout mice; knowledge; known; ko mice; labeling; lacking; lactate; large; lateral; layer; leads; learning; length; lentiviral; lesions; leukocytes; levels; ligand; light; like; like cells; likely; limited; line; lineage; lineage cells; lipid; lipopolysaccharide; lithium; littermates; little; live; local; localization; long; loss; lower; lpc; lps; ltp; lymphocytes; lysm; macrophages; main; maintenance; major; majority; male; mammalian; manner; mapk; marked; markers; marrow; mass; material; matrix; matter; maturation; mature; mbp; mcao; mcs; mct4; mean; measurements; mechanical; mechanisms; media; mediators; medium; member; membrane; memory; metabolic; metabolites; methods; mhc; mice; microarray; microenvironment; microglia; microglia activation; microglial cells; microglial processes; microglial response; microscopy; middle; migration; minocycline; mir-219; mir-302; mitochondrial; mixed; model; modifications; molecular; molecules; monocytes; months; morphological; morphology; motility; motor; mouse; mouse brain; mouse microglia; mouse model; mrna; ms patients; msc; msh; multiple; multipotent; murine; mutant; mutations; myelin; myeloid cells; naa; naag; na€; nbce1; necessary; nefiracetam; negative; neonatal; nerve; nervous; nervous system; nestin; networks; neural; neurite; neurodegenerative; neurogenesis; neurogenic; neuroinflammation; neurological; neuronal; neuronal activity; neuronal cells; neurons; neuropathic; neuropathological; neuroprotective; neurospheres; neurotransmitter; neurotrophic; new; nfjb; ng2 cells; niche; nitric; nmda; nodes; non; normal; normal brain; notch; novel; npcs; nrg; nscs; nuclear; nuclei; nucleus; number; objective; observations; observed; obtain; occlusion; occurring; oecs; ogd; oht; old; olfactory; oligodendrocytes; oligodendroglial; oligodendroglial cells; ols; ongoing; onset; opcs; open; optic; order; organotypic; osm; outcome; outer; outgrowth; overall; overexpression; oxidative; oxide; oxygen; p10; p2x; p2x4; p2y1; p75; pain; panx1; pap; parallel; parameters; parenchyma; particular; patch; pathogenesis; pathological; pathology; pathway; patients; pattern; pcr; peak; peptide; percentage; performance; perinatal; period; peripheral; perivascular; permanent; permeability; phagocytic; phagocytosis; pharmacological; phase; phenomenon; phenotype; phosphorylation; photon; physiological; physiology; plaques; plasma; plasmin; plasminogen; plasticity; play; plp; pluripotent; pns; pnv; point; population; positive astrocytes; positive cells; possibility; possible; post; postnatal; potassium; potential; precursor cells; precursors; preliminary; presence; present; present study; previous; primary; primary astrocytes; primary microglia; priming; prior; processes; processing; production; profile; profound; progenitor cells; progenitors; program; progression; proliferate; proliferation; prominent; promising; promoter; properties; proportion; protein; protein expression; protein levels; proteomic; protocol; pups; pure; purified; purinergic; purpose; putative; qpcr; quantification; quantitative; question; radial; range; rapid; rat astrocytes; rat brain; rate; rats; reaction; reactive; reactive astrocytes; reactive microglia; real; recent; receptor activation; receptor expression; receptors; recombined cells; recordings; recovery; recruitment; reduced; regenerative; regions; regulated; regulation; regulatory; related; relative; release; relevance; relevant; removal; remyelination; repair; reperfusion; reported; research; resident; resolution; respiratory; response; responsible; restricted; results; retinal; rise; robust; rodent; role; root; s100b; saline; samples; scar; schwann cells; sci; sciatic; sclerosis; second; secondary; secretion; sections; sedentary; selective; self; sensitive; sensitivity; sensory; serine; severe; sgcs; sham; sheath; short; shows; siglec; signaling; signals; significant; similar; single; single cell; sirt1; site; situ; size; sleep; slices; small; sod1; sodium; soluble; somatosensory; sorted; sorting; source; sox10; space; spatial; species; specific; specific cell; spinal; spinal cord; spinal microglia; spontaneous; sr101; sra; stable; stages; staining; state; status; stem cells; step; stimulated; stimulation; stimuli; strategies; strategy; stress; striatal; striatum; stroke; strong; structure; studies; study; subjects; subpopulation; subsequent; substances; substrate; subtypes; subunit; suitable; summary; support; suppression; surface; survival; sustained; svz; svz cells; symptomatic; symptoms; synapses; synaptic; synthase; synthesis; system; systemic; t cells; tace; tamoxifen; target; targeting; tau; technique; terc; term; terminal; test; tg mice; tg2; tgf; therapeutic; therapies; therapy; thickness; time; timp-1; tims; tissue; tlr2; tlr3; tlr4; tnf; tnfa; toll; tool; total; toxic; trafficking; transcription; transcripts; transfection; transgenic mice; transient; transmission; transplantation; transplanted; transporter; trauma; traumatic; treatment; trem2; trif; trkb; tumor; tumor cells; turn; type; type mice; typical; uller; unclear; undergo; underlying; understanding; understood; unique; united; university; unknown; untreated; uptake; values; variety; vascular; vector; vegf; vessels; viability; vitro; vivo; vlcfa; voltage; volume; vwm; water; weeks; weight; western; white; wild; wistar; wnt; work; wound; wt mice; yfp; young; zebrafish; zone; ıve
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        item: #11 of 41
          id: cord-023211-kt5gt26t
      author: None
       title: Poster Session Abstracts
        date: 2007-08-29
       words: 221712
      flesch: 48
     summary: Understanding how its modulation modifies transepithelial transport and inflammation in CF versus non CF cells should give new therapeutic clues to reduce exaggerated inflammation and improve fluid secretion in CF patients. This finding indicates that CFTR potentiators have a wide efficacy on many class III mutants and therefore may represent a promising therapeutic strategy for a significant number of CF patients.
    keywords: 1,2; 16hbe; 2004; a2b; a549; aat; abc; ability; abnormal; abnormalities; abpa; absence; absorption; access; account; accumulation; accurate; acid; acidification; acinar; acinar cells; acquisition; acting; activators; active; activities; acts; acute; addition; address; adenosine; adequate; adherence; adherent; adjusted; administration; admission; ado; adolescents; adult cf; adult cystic; adult patients; adults; advance; adverse; aerosol; aeruginosa; aeruginosa lung; affected; affinity; agar; age; aged; agents; aggressive; agonists; agreement; aim; aims; airflow; airway cells; airway disease; airway epithelial; airway infection; airway inflammation; airway surface; airways; algorithm; allele; alpha; alterations; altered; alternative; alveolar; amikacin; amiloride; amino; aminoglycoside; amounts; anaerobes; anaerobic; analysis; anemia; anemic patients; anion; annual; antibiotic; antibiotic treatment; antibodies; antibody; antimicrobial; antisense; anxiety; apical; application; appointments; approach; appropriate; areas; arikace; array; asl; aspects; aspergillus; assay; assessment; associated; association; asthma; atp; atpase; attention; aureus; australia; available; average; azithromycin; azm; background; bacterial; bal; balance; balf; band; barriers; baseline; basis; basolateral; bath; bcc; beginning; behavior; beneficial; benefit; best; beta; better; bhk cells; bile; binding; biochemical; biofilm; biological; biomarkers; birth; bles; block; blood; blot; bmi; body; bone; breathing; bronchial epithelial; bronchiectasis patients; bronchoalveolar; burden; burkholderia; ca2; cacc; calcium; calls; calu-3; camp; canada; canadian cf; cancer; capable; capacity; carbachol; caregivers; carolina; cascade; cases; categories; caucasian cf; cause; cbavd; cbf; cell lines; cell surface; centers; central; centres; cepacia; cepacia cf; cepacia patients; ceramide; certain; cf ages; cf airway; cf animals; cf association; cf balf; cf boys; cf bronchial; cf bronchiectasis; cf care; cf carrier; cf cases; cf cells; cf center; cf centre; cf children; cf clinic; cf cultures; cf data; cf database; cf diagnosis; cf disease; cf ebv; cf epithelial; cf families; cf family; cf females; cf foundation; cf gene; cf group; cf hbe; cf infants; cf isolates; cf liver; cf lung; cf macrophages; cf mice; cf model; cf mouse; cf mutation; cf nasal; cf nbs; cf neutrophils; cf offspring; cf outcomes; cf pathophysiology; cf patients; cf phenotype; cf phone; cf physician; cf population; cf practitioners; cf program; cf pulmonary; cf quality; cf registry; cf research; cf respiratory; cf samples; cf secretion; cf sputum; cf subjects; cf survival; cf team; cf therapeutics; cf therapies; cf therapy; cf transmembrane; cf treatment; cf trust; cf unit; cf vitamins; cfbe41o; cff; cfft; cfld; cfq; cfrd; cftr; cftr activation; cftr activity; cftr biogenesis; cftr cdna; cftr cell; cftr channel; cftr chloride; cftr correctors; cftr deficient; cftr degradation; cftr dependent; cftr endocytosis; cftr expression; cftr folding; cftr function; cftr gene; cftr genotype; cftr inhibitor; cftr interaction; cftr intracellular; cftr knockout; cftr levels; cftr locus; cftr maturation; cftr mice; cftr mrna; cftr mutations; cftr null; cftr orthologue; cftr potentiators; cftr processing; cftr promoter; cftr protein; cftr regulation; cftr results; cftr sequence; cftr surface; cftr tm1unc; cftr trafficking; cftrinh-172; cfu; challenge; chamber; changes; channel; channel activity; channel function; chapel; chaperones; characteristics; characterized; chart; chemical; chest; chicago; chip; chloramphenicol; cholesterol; chromatography; chronic; chronic lung; chs cf; ciliated; ciliated cells; ciprofloxacin; circuit; classes; classic cf; classified; clchannel; clearance; clinical; clinical data; clinics; clonal; clones; cm2; coated; code; coding; codon; cohort; collagen; collection; colonic; colonization; colonized; combination; combined; commd1; common; common cf; communication; community; comparable; comparison; complete; completion; complex; compliance; complications; component; composition; compounds; concentrations; conclusion; conditions; conductance; configuration; confocal; conformational; consecutive; consecutive patients; consensus; consequences; consistent; constructs; containing; content; continued; contraction; contrast; contribution; control; control cells; control patients; conventional; correctors; correlated; correlation; corresponding; cost; cough; counseling; counts; course; cpg; creatinine; criteria; critical; cross; crp; crucial; csf; csp; cst; cultures; current; cycle; cystic fibrosis; cytochalasin; cytokine; cytoplasmic; cytosolic; daily; damage; data; date; day; days; death; decision; decline; decreased; defect; defective cftr; defense; deferens; deficient; deficient patients; degradation; degree; deletion; delf508 cftr; delivery; delta; deltaf508; density; denufosol; department; dependent; deposition; depression; desensitization; design; detectable; detection; determination; determined; developed; development; deviation; device; df508; dha; diabetes; diabetic; diagnosis; diet; dietary; differences; different; different cf; differential; differentiated; difficult; diminished; direct; discharge; discussion; disease; disruption; distinct; distribution; diverse; dna; dnase; dogs; domain; dominant; dose; dosing; double; downstream; drug; dry; duox; duration; dx-890; dxa; dysfunction; e.g.; earlier; early; ebc; ednra; effect; effective; effectiveness; efficacy; efficiency; efflux; effort; egfr; elastase; electronic; electrophoresis; elements; elevated; elevation; elisa; enac; encoding; endocytic; endogenous; endogenous cftr; endoplasmic; energy; enhanced; entire; entry; environment; enzyme; epidemiology; episodes; epithelial cells; epithelium; eps; equivalent; equol; essential; established; estimate; estrogen; eur-1008; evaluation; events; evidence; exacerbation; example; excessive; exchange; exercise; exhibit; exon; expansion; expected; experienced; experiments; expiratory; export; exposure; expressing; expression levels; extent; extracts; f508del; f508del cftr; factors; failure; families; family; fasting; fat; fatty; features; fed; fev1; fewer; ffm; fibrosis airway; fibrosis center; fibrosis foundation; fibrosis lung; fibrosis patients; fibrosis transmembrane; final; findings; flagellin; flow; fluid; fluorescence; focus; fold; folding; following; food; forms; forskolin; fractions; france; free; french cf; frequency; frequent; frt; frt cells; fumigatus; functional; functioning; fungal; future; fvc; fxyd5; g551d; gain; gamma; gastrointestinal; gastrostomy; gating; gel; gels; gender; gene expression; gene therapy; general; generation; genes; genetic; genistein; genome; genomic; genotype; genotyping; germany; gfp; girls; glands; global; glucose; glutathione; goal; good; gradient; grant; greater; green; groups; growth; gs-9310/11; gsh; guidelines; h2o; half; halide; hand; hbac cftr; hbe; hbe cells; hbecs; hdj-2; health; healthcare; hearing; hela; hela cells; help; heterozygous; high; higher; highest; hill; hispanic cf; hispanic patients; history; hits; hmgb1; home; homeostasis; homozygous; homozygous cf; hospital; hospitalization; host; hours; hrct; hrqol; hrs; hsp70; human airway; human cells; human cf; human lung; human patients; humans; hyperabsorption; hypertonic; hypoglycaemia; hypothesis; hypothesized; hypoxia; i.v; ib3; ib3 cells; ibuprofen; identical; identification; identifying; ige; igg; iii; il-10; il-17a; il-23; il-6; il-8; illness; images; imaging; immature cftr; immune; immunofluorescence; immunoprecipitation; impact; impaired; impairment; important; important cf; improved; improvement; incidence; income; increase; incubated; incubation; index; individuals; induced; induction; infected; infection; inflammation; inflammatory; inflammatory cells; influence; influx; information; inhalation; inhaled; inhibited; inhibition; inhibitors; initial; initiation; injury; innate; inserm; insights; institute; instrument; insufficiency; insulin; insurance; intact; intake; intensity; interaction; interest; interface; interim; intermediate; internal; internalized cftr; intervention; interviews; intestinal; intracellular; intravenous; introduction; investigation; involvement; iodide; irt; isc; isolates; isolation; issues; italian cf; italy; items; jet; junctions; kda; key; killing; kinase; kingdom; knockdown; knowledge; known; laboratories; laboratory; lack; lactate; large; lavage; layer; leading; leads; left; legal; length; lentiviral; levels; levofloxacin; library; life; likely; limited; linear; lines; lipid; liposomal; liposomes; liquid; list; literature; littermates; little; local; localization; localized; london; long; longer; longitudinal; loss; lower; lps; luciferase; lumen; luminal; lung cells; lung disease; lung epithelial; lung function; lung infection; lung inflammation; lung transplant; lungs; lymphoma; magnitude; maintenance; major; major cf; majority; making; malabsorption; male cf; malh; management; mannitol; marked; markers; mass; material; matrix; mature; maximal; maximum; mcc; mdck; mean; mean age; mean fev1; measure; measurements; mechanism; median; mediators; medical; medication; medicine; medium; members; membrane; membrane cftr; membrane expression; metabolism; methods; microbiology; micrornas; microscopy; migration; mild; mild cf; min.gland; mineral; minimal; minutes; missed; missense; mixed; mmol; mmp-8; model; moderate; modest; modifier; molecular; molecule; monitoring; monolayers; months; morbidity; mortality; motif; motility; motor; mouse lung; mp-376; mrna; mrsa; muc4; muc5ac; mucin; mucociliary; mucoid; mucosal; mucous cells; mucus; multicenter; multiple; murine; mutant cftr; mutants; n=10; n=3; n=4; n=5; n=6; n=7; n=9; na+; nacl; nanoparticles; nasal; national; nbd1; nbd2; nbds; nebulization; nebulizer; necessary; need; negative; neonatal; neutrophil; new; new cf; newborn; nherf1; nih; non; non cf; normal; normal cftr; north; novel; npt2a; nthi; nuclear; nucleolin; nucleotide; number; numerous; nutritional; objective; observation; observed; obstruction; ogtt; oil; ongoing; onset; oocytes; open; optimal; oral; order; organisms; outcomes; outpatient; output; overall; overexpression; overnight; oxalate; oxidative; oxygen; p2y; p<0.001; p<0.05; padi; pain; pairs; pancreatic; panel; pao1; parallel; parameters; parental; parents; participants; participation; particular; past; patch; pathogenesis; pathogens; pathology; pathway; patients age; pattern; pbs; pcd; pcn; pcr; pdz; peak; pediatric cf; pediatric patients; pediatrics; peg; people; peptide; percent; percentile; performance; perfusion; period; peritoneal cf; permeability; persistent; pex; pfge; pfts; pge2; pgp; pharmacokinetic; pharmacological; phase; phenotypes; phenylalanine; phosphorylation; physical; physicians; physiological; physiotherapy; picc; pigs; pilot; pka; pkc; placebo; placement; planktonic; plasma; plasmid; play; pmns; point; polarized; polymorphisms; poor; populations; porcine; porcine cftr; positive; positive cells; positive cf; possible; post; potency; potential; pparγ; practice; predominant; pregnancy; pregnant; preliminary; preliminary results; prescribed; presence; present; present study; pretreatment; prevalence; previous; primary; primary cf; prior; probability; probe; problem; procedures; process; processes; processing; production; products; profile; program; progression; project; proliferation; prolonged; prominent; promising; properties; proportion; prospective; prostasin; protease; protein expression; protein levels; proteins; proteolytic; protocol; providers; ps patients; psa; pseudomonas; pseudomonas aeruginosa; pulmonary disease; pulmonary exacerbation; pulmonary function; pulse; pups; purified; purpose; putative; pyocyanin; qds; quality; quantitative; quarterly; questionnaire; questions; raav; rab4; radiation; randomized; range; rapid; rare; rate; ratios; rats; ray; reactions; reactive; reasons; recent; receptor; recipients; recombinant; recommendations; recorded; records; recovery; recruitment; recurrent; recycling; redox; reduced; redundancy; reference; regimens; region; regular; regulated; regulation; regulatory; related; relationship; relative; relaxation; release; relevance; relevant; renal; repeat; reported; reporting; reports; reproductive; rescue; residue; resistance; respect; respondents; response; responsible; results; retention; reticulum; retrospective; review; risk; role; routine; royal; rsv; safety; saline; salt; samples; satisfaction; saturation; scale; scanning; scans; school; sciences; scores; screened; screening; sds; second; secondary; secrete; secretion; secretory; sectional; selective; self; sensitive; sensitivity; separate; sequence; sequencing; serca; series; serosal; serous; serous cells; serum; set; setting; severe; severe cf; severe lung; severity; sex; sft; short; shrinkage; signaling; significant; significant increase; signs; similar; single; sinus; sirna; site; size; skin; slc26a9; sleep; slope; slow; small; smm; snps; social; sodium; software; solids; soluble; solution; source; species; specific; specific cftr; specificity; specimens; spectrometry; spirometry; splicing; spp; stability; stable cf; stable patients; staff; staining; standard; standard cf; standardized; start; state; statistical; status; steady; stem cells; step; stimulated; stimulation; stip-1; stone; strains; strategies; strategy; stress; strong; structure; studies; study; study group; study patients; study period; stx6; submucosal; subsequent; substantial; substrate; subunit; successful; sufficient; suitable; sult1e1; summary; supernatant; supplementation; support; surface; surface expression; surfactant; surgery; survey; survival; susceptibility; sustained; sweat; symptoms; synagis; synergy; synthesis; system; systemic; t84; target; targeted; targeting; techniques; technology; temperature; terminal; terminus; test; tested; testing; tgf; tgfβ; therapeutic; therapies; therapy; thought; throughput; tidal; tight; time; tissue; tlr2; tnfα; tobramycin; tolerance; tolerated; tool; total; total cells; total cftr; toxicity; tracheas; tract; training; transcription; transduced; transduction; transepithelial; transfected; transfer; transgene; transient; transition; transplant; transplant patients; transplantation; transport; transporters; treatment; trend; trials; tube; type cftr; types; ubiquitin; udca; uk cf; unable; unchanged; unclear; underlying; understanding; underwent; unique; united; university; unknown; untreated; upr; uptake; urea; usa; useful; ussing; values; variability; variable; variants; variation; variety; vas; vcp; vector; vehicle; ventilation; vestibular; vip; viral; virulence; virus; viscosity; visits; vitamin; vitro; vivo; voltage; volume; volunteers; voriconazole; waiting; wall; water; weeks; weight; western; wide; wild; wildtype; women; work; worse; wt cells; wt cftr; wt mice; xbp-1s; years; young; young cf; young patients; younger; yrs; zone; ∆264; ∆f508 cftr; ∆f508cftr
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        item: #12 of 41
          id: cord-102184-8u73adnk
      author: Li, Jinzhi
       title: Visual input into the Drosophila melanogaster mushroom body
        date: 2020-05-02
       words: 8719
      flesch: 47
     summary: We have a thorough understanding of how olfactory Kenyon cells integrate input from the 470 antennal lobe: most Kenyon cells receive, on average, input from seven projection neurons and the projection neurons connecting to the same Kenyon cell share no apparent common features (Caron et al., 2013; One to three-day-old flies were used when mapping the connectivity rate between input neurons 570 and α/βp Kenyon cells.
    keywords: accessory calyx; antennal; body; bp kenyon; brain; calyx input; centers; cluster; combination; connectivity; dendrites; different; dorsal accessory; drosophila; dye; et al; expression; figure; gal4; gfp; grasp; individual; information; input; input neurons; kenyon cells; labeling; lateral; lateral protocerebrum; like; lines; lobe; lobula; lopn; main; main calyx; medulla; melanogaster; mushroom; neurons; number; olfactory; photo; plppns; posterior; processing; projection; projection neurons; protocerebrum; r19h07; region; screen; sensory; study; superior; synaptic; system; table; technique; terminals; transgenic; transgenic lines; types; uas; unknown; ventral; visual
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        item: #13 of 41
          id: cord-103509-hynnba03
      author: Wong, Ten-Tsao
       title: A self-assembled protein nanoparticle serving as a one-shot vaccine carrier
        date: 2020-09-18
       words: 3235
      flesch: 44
     summary: As shown in figure 1A , when GFP protein with a molecular weight of 27kDa is able to pass through membranes with MWCO of 100kDa, 300kDa and 1000kDa freely, AH3-GFP fusion protein purified from bacterial lysate was prevented from passing through the membrane with an MWCO of 1000kDa. GFP protein has a thermal stable structure that constituted by 11 β-strands and 3 α-helices and some of the loops between strands have been explored as insertion sites for heterologous protein for various purposes (18, 19, 30) .
    keywords: ah3; amphipathic; antibody; antigenicity; barrel; carrier; complex; day; dimer; fig; fluorescent; function; fusion; gfp; helical; heterologous; high; hm2e; hydrophobic; igg; immune; immunization; interaction; like; long; membrane; mice; nanoparticle; patch; peptide; protein; recombinant; responses; results; sfgfp; sfgfp-2xhm2e; single; stability; stable; structure; tem; terminal; titer; vaccine; virus; vlp
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        item: #14 of 41
          id: cord-103868-iwpiti2h
      author: Harrison, Angela R.
       title: The Ebola virus interferon antagonist VP24 undergoes active nucleocytoplasmic trafficking
        date: 2020-08-11
       words: 2265
      flesch: 35
     summary: Nuclear export is not required for STAT1 antagonism, consistent with competitive karyopherin binding being the principal antagonistic mechanism while export mediates return of nuclear VP24 to the cytoplasm for replication functions. Nuclear export is not 27 required for STAT1 antagonism, consistent with competitive karyopherin binding being the 28 principal antagonistic mechanism while export mediates return of nuclear VP24 to the 29 cytoplasm for replication functions.
    keywords: 251; active; cells; consistent; crm1; cytoplasmic; dependent; ebola; ebov; export; figure; gfp; ifn; karyopherin; lmb; localisation; mechanism; nes; ness; nuclear; nucleus; proteins; replication; sequences; stat1; terminal; trafficking; virus; vp24
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        item: #15 of 41
          id: cord-253709-frauasts
      author: Lan, Dongming
       title: An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases
        date: 2011-08-15
       words: 2067
      flesch: 46
     summary: Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography. Here we demonstrate the utility of the ELP-tagged proteases for cleavage of ELP fusion proteins, allowing one-step removal of the cleaved ELP tag and ELP-tagged proteases without chromatography.
    keywords: cell; chromatography; cleavage; cleaved; elastin; elp; elp-3c; expression; fig; free; fusion; gfp; itc; lane; like; method; polypeptide; protease; proteins; purification; purified; recombinant; step; sumo; tag; target; transition
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        item: #16 of 41
          id: cord-257167-rz4r5sj7
      author: None
       title: Abstracts for the 29th Annual Meeting of the Japan Neuroscience Society (Neuroscience2006)
        date: 2006-12-31
       words: 241480
      flesch: 43
     summary: Inst., Kyoto Univ., Inuyama, Japan GABAergic cells in the cerebral cortex are divided into subgroups: parvalbumin (PV)-, somatostatin (SOM)-, calretinin (CR)-, and calbindin-containing types. In the present study, we therefore investigated the synthesis of sex steroids and corticosteroids in the hippocampus of adult male rats, by monitoring the metabolism of tritiated steroids with HPLC system. PS2A-G125 GABA depolarizes GnRH neurons isolated from adult GnRH-EGFP transgenic rats Chengzhu Yin, Nobuyuki Tanaka, Masakatsu Kato, Yasuo Sakuma Nippon Medical School, Department of physiology, Tokyo, Japan GnRH neurons are essential in the reproductive neuroendocrine system.
    keywords: -catenin; 1,2; 1,2,3; 1,3; 1,4; 1,5; 100; 2,3; 2,4; 21st; 5ht; aaf; abdominal; abe 1; ability; abnormal; abnormalities; abnormality; absence; absent; abundant; acceleration; accumulation; ache; acid; acoustic; acquisition; acsf; action; activated; activation; active; activin; activities; activity; activity dependent; acute; adaptation; addition; address; adenosine; adenoviral; adhesion; adjacent; administration; adrenal; adult; adult brain; adult mice; adulthood; advanced; affected; affects; afferent; affinity; age; aged; agency; agent; aggregates; agonists; agriculture; aichi; aid; aim; aist; akiko; akira; akt; alpha; alteration; altered; alzheimer; ambiguous; amino; amounts; ampar; amph; amplitude; amygdala; amygdala neurons; amyloid; analysis; anatomical; anatomy; androgen; anesthetized; angle; animals; antagonist; anterior; anterograde; anti; antibodies; antibody; antisense; anxiety; aob neurons; aoki 1; apical; apoptosis; apparent; application; applied; approach; architecture; area; arginine; array; artificial; aspects; assay; assembly; associated; association; astrocytes; astroglial; asymmetric; atp; atsushi; attached; attention; auditory; auditory cortex; autism; available; average; aversion; aversive; avoidance; avp; axis; axonal; axons; background; balance; band; barrel; barrier; basal; basis; bath; battery; bdnf; behavioral; behaviour; belt; best; beta; better; bicuculline; bilateral; binding; binocular; biochemical; biochemistry; biological; biology; biomedical; biophysics; bioscience; bipedal; bipolar; birds; birth; bisphenol; bla; blind; blockade; blocker; blocking; blocks; blood; blot; blue; bmal1; bmp; bmp4; bodies; body; bold; box; bpa; brain; brain activation; brain activity; brain areas; brain cells; brain development; brain function; brain injury; brain neurons; brain regions; brain research; brain science; brainstem; branches; branching; brdu; brief; bsi; bulb; burst; bursting; c57bl/6; ca1; ca3; cage; calbindin; calcium; callosum; calpain; camkii; camkk; cancer; candidate; cantor; capable; capsaicin; carbachol; care; case; categories; cats; caudal; causes; cbln1; cdna; cell activity; cell adhesion; cell biology; cell bodies; cell death; cell layer; cell line; cell migration; cell proliferation; cell surface; cell synapses; cell types; cells; cellular; center; central; cerebellar; cerebral; cerebral cortex; certain; chain; changes; channel; characteristics; characterization; characterized; chemical; chewing; chiba; chiba university; chick; children; chip; chloride; choices; cholesterol; cholinergic; cholinergic neurons; chondroitin; chromosome; chronic; cingulate; circadian; circuit; circuitry; clamp; class; classical; classifier; cleavage; clinical; clock; clorgyline; close; cluster; clustering; cnqx; cns; cns neurons; co.; coculture; coe; cognitive; cold; college; colliculus; color; combination; combined; common; communication; comparable; comparison; compartment; complementary; complete; complex; complex cells; complexes; complexity; component; compounds; comprehensive; computational; computer; concentrated; concentration; conclusion; condition; conditioning; conductance; cone; configuration; confocal; connected; connections; connectivity; conscious; conserved; consistent; consists; constant; contacts; context; contextual; continuous; contraction; contralateral; contrast; contribution; control; control mice; conventional; convergence; conversion; cooling; coordination; core; correct; correlated; correlation; corresponding; cortex; cortex neurons; cortical activity; cortical cells; cortical neurons; cortices; corticospinal; corticosterone; course; courtship; cox-2; cpa; cpp; creb; crest; crf; critical; crmp-2; cross; crucial; csf; cues; cultivation; cultured; cultured cells; cultured neurons; current; curve; cycle; cytokines; cytoplasmic; cytoskeleton; da neurons; daily; damage; dark; data; days; death; decision; decreased; decreasing; deep; defective; defects; deficient; deficient mice; deficient neurons; deficit; degradation; degree; delay; delivery; dementia; demyelination; dendrites; dendritic; dense; densities; density; dental; dental university; dentate; dentistry; department; dependent; depletion; depolarization; depolarized; depolarizing; depressed; deprivation; deprived; dept; desensitization; detailed; detection; determined; developed; development; dex; dha; diet; differences; different; differential; differentiation; difficult; diffusion; dimensional; direction; disc1; discharges; discrete; discrimination; disease; disorders; disparity; display; disruption; distal; distance; distant; distinct; distribution; div; diverse; division; dna; domain; dominance; dominant; dopaminergic neurons; dorsal; dose; dots; double; downstream; drebrin; drg; drosophila; drpla; drug; duration; dye; dynamics; dysbindin; dysfunction; dystonia; early; ectopic; education; eeg; effect; effective; efficacy; efficiency; egfp; ehime; electrical; electrode; electron; electrophysiological; electroporation; elegans; element; elevated; elimination; elongation; embryonic; embryos; emg; emotional; encodes; encoding; endocrine; endogenous; energy; engineering; english; enhanced; enhancement; entire; entorhinal; entry; environmental; enzyme; epigenetic; epileptic; epscs; epsp; erk; erk5; error; es cells; essential; establishment; estimation; estradiol; estrogen; ethanol; ethical; evaluation; events; evidence; evoked; example; excitability; excitation; excitatory; excitatory neurons; execution; exercise; exhibit; existence; exocytosis; experience; experiments; exposure; expression; expression levels; extension; extensive; extent; external; extinction; eye; eyes; faces; facial; facilitation; factors; faculty; family; fast; fatty; fear; features; fed; feedback; feeding; female; fetal; fibers; field; filopodia; filtering; finches; findings; fine; finger; firing; fixation; fixed; flavoprotein; flies; flocculus; flow; fluid; fluorescence; fmri; fmri study; focused; following; food; foot; forebrain; formation; forms; forward; fos; fos expression; foundation; fraction; free; frequency; frontier; fujita; fukuda; fukui; fukuoka; fukushima; function; fundamental; funds; future; fyn; gaba; gabaergic cells; gabaergic neurons; gad67; gain; gamma; ganglia; ganglion; ganglion cells; ganglion neurons; gap; gaze; gdnf; gel; gene; gene expression; general; generate; genetic; geniculate; genome; genomic; gfap; gfp; gifu; glial; glial cells; global; glomeruli; glucocorticoid; glucose; glur; glutamate; glutamate receptor; glutamatergic; glutathione; glycine; glycinergic; gnrh neurons; gonadal; good; gpi; gpi neurons; gradient; graduate; graduate school; grant; granular; granule cells; granule neurons; grasping; gray; greater; green; grip; group; growth; gsh; gtpases; guidance; guinea; gunma; gyrus; habituation; half; hand; hashimoto; hattori; hayashi; head; health; heat; heavy; hemifield; hemisphere; hemodynamic; hemoglobin; hepatic; hes1; heterozygous; hgf; hideki; hideyuki; high; higher; hindbrain; hippocampal cells; hippocampal neurons; hippocampus; hiroaki; hiroko; hironori; hiroshi; hiroshima; hiroyuki; hokkaido; hokkaido university; home; homologue; horie; horizontal; hormone; horn; hospital; host; hpa; human; human brain; hundreds; hvc; hybridization; hydroxylase; hyogo; hyperactivity; hyperalgesia; hypoglossal; hypothalamic; hypothermia; hypothesis; i.p; ibaraki; ichiro; idea; identical; identification; ifenprodil; iii; ikuko; image; imaging; immature; immediate; immune; immunoglobulin; immunohistochemistry; immunopositive; immunoreactive neurons; immunoreactivity; immunostaining; impact; impaired; impairment; impedance; implicit; important; important role; improved; inclusions; increased; index; india; individual; individual neurons; induced; induction; industrial; infant; infected; inferior; inferotemporal; inflammation; inflammatory; influence; influx; infrared; infusion; inhibited; inhibition; inhibitory; inhibitory neurons; inhibits; initial; initiation; injection; injured; injury; inner; innervation; inoue; inputs; insights; institute; instructed; insular; insulin; insults; intact; intake; integrated; integrative; intensity; interaction; intermediate; interneurons; interval; intracellular; intraperitoneal; intrinsic; invasive; investigation; involvement; inward; ipsilateral; iran; irradiation; ischemia; ishii; ishikawa; isoforms; isolated; isolation; ito 1; iwate; izumi; japan; japan activation; japan application; japan astrocytes; japan axons; japan brain; japan c.; japan calcium; japan cerebellar; japan cortical; japan dendritic; japan distribution; japan dopaminergic; japan early; japan effects; japan expression; japan gaba; japan gabaergic; japan glial; japan glutamate; japan gnrh; japan high; japan layer; japan long; japan mammalian; japan memory; japan mice; japan microglia; japan multiple; japan neural; japan neurons; japan olfactory; japan postsynaptic; japan previous; japan ps3p; japan purpose; japan recent; japan science; japan society; japan stimulation; japan synaptic; japan vesicular; japan visual; japanese; jikei; jsps; jst; jun; junctions; junichi; juntendo; kagaku; kagoshima; kainate; kakenhi; kanagawa; kaneko; kaoru; kato; katsuhiko; kawaguchi; kawasaki; kawashima; kawato; kazunori; kazuo; keio; keio univ; keisuke; kenji; khz; kida; kimura; kinase; kinds; kinesin-1; kinetics; kinki; kit; kitakyushu; kiyokazu; knockdown; knockout; knockout mice; knowledge; known; ko mice; kobayashi; kobe; koichi; kubo; kumamoto; kunihiko; kurume; kyoto; kyoto university; kyushu; lab; labeling; laboratory; lacking; lactate; lamina neurons; laminar; language; lapse; large; latency; lateral; layer; leading; learning; left; length; leptin; lesion; lesioned; level; lgn; lgn neurons; life; ligand; like; likely; limbic; limited; line; linear; lingo-1; lipid; little; lobe; localization; localized; locations; locomotor; locomotor activity; locus; long; longer; loop; loss; lower; lpa; lpfc; ltd; ltm; ltp; macaque; maebashi; maeda; magnetic; magnitude; maintenance; major; majority; making; makoto; male; mammalian; mammalian brain; mammals; manabu; manipulation; manner; map1b; mapk; mapping; maps; markedly; marker; masaharu; masahiko; masami; masanori; masato; masayuki; mass; matching; maternal; matrix; matsuda; matsumoto; matter; maturation; mature neurons; maze; mdma; mean; measurement; measuring; mechanical; mechanisms; mecp2; med; mediate; mediator; medical; medical university; medicine; medium; medulla; meeting; meg; member; membrane; memory; metabolism; metabolites; metastin; method; methylation; metropolitan; mext; mfr; mfs; mglur1; mice; mice brain; microarray; microelectrode; microglia; microscopy; microtubule; midbrain; middle; migrating; migration; migratory; mild; milliseconds; mind; minoru; mitochondrial; mitral; mitsubishi; miura; miyazaki; mk-801; model; model system; modification; modified; modulated; modulation; molecular; molecular mechanisms; molecules; monitoring; monkeys; monoamine; monocular; months; mori; morphine; morphogenesis; morphological; morphology; mossy; motif; motion; motor; motor cortex; motor neurons; mouse; mouse brain; movement; moving; mpfc; mptp; mrna; multiple; murakami; muscimol; muscle; muscular; mushroom; music; mutant; mutant mice; mutation; myelin; myelinated; myoclonus; nacl; nagasaki; nagoya; nagoya university; nakajima 1; nakamura; naoki; nara; national; national institute; native; natl; natural; nature; ncam; ncnp; near; necessary; need; negative; neocortex; neocortical; neonatal; nerve; nervous; nervous system; nestin; netrin; netrin-1; network; neun; neural; neural activities; neural cell; neural development; neural mechanisms; neural stem; neurite; neurobiology; neuroblasts; neurodegenerative; neuroendocrine; neuroethics; neurogenesis; neurogenic; neuroimaging; neurological; neurology; neuronal; neuronal activity; neuronal cells; neuronal differentiation; neuronal function; neuronal migration; neuronal responses; neurons; neuropathic; neurophysiology; neuroprotective; neuroscience; neurospheres; neurosurgery; neurotoxicity; neurotransmitter; neurotrophic; new; newborn; ng108; ng2; ngf; nicotine; nigra; nihon; nihon university; niigata; niigata university; nippon; nips; nirs; nitric; nmda; nmda receptor; nobuyuki; nociceptive; noise; nomura; non; nonlinear; noriko; normal; notch; novel; novo; noxious; npy; nr1; nr2b; nscs; ntt; nuclear; nuclei; nucleotide; nucleus; number; obata; object; objective; observation; observed; obvious; occipital; odor; odorant; ogawa; ohda; okamoto; okamoto 1; okayama; okazaki; old; olfactory; olfactory neurons; olig2; oligodendrocytes; ones; ono; ono 1; onset; open; opposite; optical; optokinetic; order; order neurons; orexin; orexin neurons; organization; organotypic; organs; orientation; origin; original; os2a-8; os3p-4; osaka; osaka university; osamu; oscillations; oscillatory; outcome; outgrowth; output; outward; overexpression; ovx; oxidation; oxidative; oxide; oxt; oxy; oxygen; p2x; p2y; p38; pain; pairs; pallidus; paper; paradigm; parallel; parameters; paraventricular; parietal; parkinson; parkinsonian; partial; particular; parts; pass; passive; patch; pathogenesis; pathological; pathology; pathophysiology; pathway; patients; pattern; patterning; pax6; pc12 cells; pcns; pcp; pcr; pcs; pdgf; pdz; peak; peptide; perception; perceptual; performance; perfusion; perineuronal; period; periodic; peripheral; permeability; pfc; pgd; pgds; pge; pharmaceutical; pharmacological; pharmacology; phase; phasic; phenomenon; phenotype; phosphorylation; photon; photoreceptors; physiological; physiology; pi3k; pictures; pituitary; pixel; pka; pkc; place; plasma; plasticity; play; plexin; plus; pns; poa; point; polarity; polyglutamine; polyq; pool; populations; position; positive; positive cells; positive neurons; possibility; possible; posterior; postnatal; postsynaptic; postsynaptic neurons; postural; posture; potassium; potential; potentiation; powerful; ppt; practice; precerebellar neurons; precise; precursor; precursor cells; preference; preferential; prefrontal; prefrontal cortex; pregnant; premotor; prenatal; preoptic; preparations; prepared; presence; present; present results; present study; presentation; pressure; presto; presynaptic; pretreatment; previous study; primary; primate; prior; priority; probability; probe; problem; process; processes; processing; produce; profiles; progenitor cells; progenitors; program; progress; progression; projection neurons; projections; proliferation; prolonged; prominent; promoter; propagation; properties; property; proportion; proteasome; protective; protein; protein expression; proteomics; protrusions; proximal; prrp; ps1a; ps1p; ps2a; ps2p; ps3a; ps3p; psa; psd; psd-95; psychiatry; psychology; ptz; puberty; pulse; pups; purified; purkinje cells; purpose; pursuit; putamen; putative; pvn; pyramidal cells; pyramidal neurons; quantitative; question; rac1; radial; raft; random; range; raphe; rapid; rat brain; rat hippocampal; rate; rats; reaching; reaction; reactive; real; rearing; receptive; receptor; receptor activation; receptor neurons; recognition; reconsolidation; reconstructed; recorded; recording; recovery; recruitment; reduced; reduction; reelin; reflex; regeneration; region; regional; regulated; regulation; regulatory; rehabilitation; reinforcement; related; relationship; relative; relay neurons; release; relevant; remains; remodeling; removal; repetitive; replacement; report; reporter; reproductive; repulsive; required; rescue; research; research center; research funds; research institute; resident; residues; resistance; resonance; respiratory; response; responsible; responsive neurons; resting; restricted; results; retardation; retention; reticular; retinal; retrieval; retrograde; reversal; reversed; reward; rewarding; rgcs; rho; rhoa; rhythm; rhythmic; rich; right; rigidity; riken; riken brain; rise; risk; rivalry; rnai; rnd2; robust; rodent; role; root; rostral; rotation; rp58; rsna; rtms; rtt; rule; running; saccades; saccadic; sachiko; sad; saitama; sakai; sakurai; saline; salt; sapporo; sasaki; sato; sato 2; satoshi; scale; scanning; scar; schedule; schizophrenia; schl; school; sciatic; science institute; sciences; scientific; scn; scn neurons; screening; sdn; second; secondary; secretion; sections; segment; seiji; seizure; selection; selective; selectivity; self; sema3a; sema6d; semantic; sendai; sensation; sensitive; sensitivity; sensorimotor; sensory; sensory neurons; sequence; sequential; serial; series; serine; serotonergic; serotonin; serum; session; sets; severe; severity; sex; sexes; sexual; sham; shape; shift; shigeo; shigeru; shinichi; shinya; shock; short; showa; signaling; signals; significant; similar; similarity; simple; simultaneous; single; single cells; single neurons; sirna; sirt1; site; situ; situation; size; skin; sleep; slices; slow; small; small neurons; smaller; smooth; social; society; sod1; sodium; sokendai; soluble; solution; somata; somatic; somatosensory; song; sorst; sound; source; space; spatial; spatiotemporal; special; species; specific; specificity; spectroscopy; speech; speed; spike; spiking; spinal; spinal neurons; spines; spontaneous; sporadic; sports; spvi; srf; stability; stable; stages; staining; standard; start; startle; state; stationary; statistical; stem cells; step; steroids; stimulated; stimulation; stimuli; stimulus; strains; strength; stress; striatal; striatal neurons; striatum; stroke; strong; stronger; structure; students; studies; study; subcellular; subjects; subsequent; subset; substantia; substrate; subtypes; subunit; subventricular; suckling; sucrose; suguru; suita; sulcus; sulfate; sulffp1; superfamily; superficial; superior; support; suppression; surface; surgery; surround; survival; susceptibility; sustained; suzuki; svs; svz; sy1; sy2; sy3; symmetrical; sympathetic; symposium; symptoms; synapses; synaptic; synaptic activity; synaptic plasticity; synaptic transmission; synaptogenesis; synaptotagmin; synchronization; synchronous; synchrony; syndrome; syntactic; synthase; synthesis; synthesized; synuclein; system; systemic; tactile; tail; takaaki; takahashi; takahiro; takao; takashi; takayuki; takeda; talk; tanaka; target; target neurons; targeted; targeting; task; taste; taurine; tde; technique; technology; telencephalon; teleost; temperature; temporal; tendency; term; terminals; test; tested; tetsuo; tetsuya; tgf; tgl; thalamocortical; thalamus; theory; therapeutic; therapy; thermal; thermotaxis; theta; thin; thought; threshold; thrombin; thyroid; time; timing; tissue; tmt; tochigi; tohoku; tohoku university; tokushima; tokyo; tokyo institute; tokyo medical; tokyo metropolitan; tokyo university; tomoko; tomoyuki; tone; tongue; tonic; tools; toshihiko; toshio; toshiyuki; total; tottori; toxin; toyama; toyohashi; tracer; tracing; tract; tractography; trafficking; training; trajectory; transcranial; transcription; transcripts; transduction; transfer; transformation; transgenic; transgenic mice; transient; transition; translocation; transmembrane; transmission; transmitter; transplantation; transplanted; transport; transporters; treated; treatment; trg neurons; trial; trigeminal; trkb; trpm8; trpv4; tsukuba; tsuyoshi; ttx; tube; tubulin; tumor; tuning; turn; twice; type; type mice; typical; tyrosine; ubiquitin; uchida; uchida 1; ueda; unclear; underlying; understanding; understood; unique; university; university graduate; university school; unknown; upper; uptake; urethane; usa; useful; v1 neurons; vagus; value; variation; varied; variety; vascular; vasopressin; vectors; vehicle; velocity; ventrolateral; vertebrate; vesicles; vesicular; vesl-1s; vgat; vglut1; viability; view; viral; virus; visceral; visual; visual cortex; visual system; visualized; vitro; vivo; vocal; voltage; volume; voluntary; volunteers; vta; wakefulness; wako; walking; wall; waseda; waseda university; watanabe; water; wave; weak; weeks; weight; western; whisker; white; wide; wild; wistar; wnt; women; words; working; yamada; yamaguchi; yamamoto; yamashita; yamazaki; yasuo; yasushi; years; yokohama; yoshida; young; yuichi; yuji; yuko; zebrafish; zinc; zone
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        item: #17 of 41
          id: cord-258678-0atfsivf
      author: Liu, Hong Yan
       title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras
        date: 2013-11-07
       words: 4800
      flesch: 40
     summary: Unfortunately, these conventional siRNA nanocarriers are unsuitable for chimera delivery, and, in fact, reverse the signature property of chimera, simple formulation for regulatory approval and clinical translation 15, 16 . Although positively charged nanocarriers promote siRNA cell entry, it is well known that they are also quickly cleared by the RES, increase non-specific binding with cells and cytotoxicity 35 .
    keywords: aptamer; binding; block; capability; cationic; cells; chimera; clinical; complex; complexes; confocal; constructs; control; cytometry; delivery; domain; double; drug; dsrbd; dsrna; effect; endosomal; endosome; escape; expression; figure; final; flow; fluorescence; functionalities; gel; gene; gfp; hours; human; imaging; kinase; lipofectamine; lncap; medium; molecules; nanocarriers; nanoparticles; non; pcr; polyhistidine; population; positive; prostate; protein; protein tag; psma; rnai; sequence; silencing; simple; sirna; size; small; specific; strand; structure; tag; tags; targeted; targeting; terminal; therapeutic; total; translation; vector; vivo
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        item: #18 of 41
          id: cord-272018-txdc0c3j
      author: Laing, Eric D.
       title: Enhanced Autophagy Contributes to Reduced Viral Infection in Black Flying Fox Cells
        date: 2019-03-14
       words: 6911
      flesch: 41
     summary: Although we cannot yet definitively associate autophagy function with host tolerance to ABLV infection in black flying fox cells, ongoing experiments will refine our mechanistic understanding of enhanced basal autophagy and virus-induced autophagy in bat cells, and further characterize the pro-survival role of autophagy during virus infection. Enhancement of basal and virus-induced autophagy in bat cells connects related reports that long-lived species possess homeostatic processes that dampen oxidative stress and macromolecule damage.
    keywords: ablv; ablv infection; ablv replication; activation; anti; antibody; autophagosomes; autophagy; bafa1; basal; bats; bez; black; brain; cell lines; cells; cellular; collected; culture; death; dependent; dose; effects; entry; expression; figure; flux; flying; fold; fox; fox cells; gfp; hek293; high; host; hpi; human; human cells; ifn; increase; infected; infection; inhibitor; intrinsic; l cells; lc3b; levels; like; lines; lyssavirus; mechanism; moi; mtor; nbf; ndp52; neuroblastoma; p62; pabrh; pabrh cells; pakit; pathogenic; pathway; percentage; polyclonal; positive; post; potential; protein; pteropus; rabies; rapa; replication; significant; smer; species; study; titers; treatment; type; usa; vgfp; viral; virus; viruses
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        item: #19 of 41
          id: cord-273711-bxijla09
      author: Zhao, Zhixun
       title: RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells
        date: 2012-02-17
       words: 4185
      flesch: 46
     summary: Transfection of the siRNA expression cassettes into BHK-21 cells BHK-21 cells were seeded in six-well plates and cultured at 37°C and 5% CO2 overnight. To determine transfection efficiency, we monitored GFP fluorescence intensity of transfected cells using fluorescent microscope analysis.
    keywords: analysis; cassettes; cells; control; disease; effect; effective; egfp; expression; figure; gene; gfp; goatpox; gtpv; hairpin; human; infected; infection; inhibition; interference; mammalian; medium; mrna; new; orf095; p165; p296; p61; p70; pcr; plasmids; post; primer; promoter; protein; real; replication; results; rna; rnai; sequence; silencing; sirna; specific; study; target; time; transfected; transfection; vector; vero; viral; virion; virus; viruses
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        item: #20 of 41
          id: cord-274210-uaaj66xq
      author: Ding, Ning
       title: Induction of Atypical Autophagy by Porcine Hemagglutinating Encephalomyelitis Virus Contributes to Viral Replication
        date: 2017-02-28
       words: 5165
      flesch: 40
     summary: It indicated that the complete autophagy pathway was activated after treatment with rapamycin; although, PHEV infection blocked the smooth of autophagic flow, PHEV replication was also inhibited because of the rapamycin treatment. Neuro-2a cells were infected with PHEV (10 5.43 TCID 50 /mL) in cell cultures with 2% FBS for 1 h. Following a 1 h absorption period, infected cells were incubated in the complete DMEM at 37 • C for the relevant times in the conformity with experimental requirements.
    keywords: accumulation; antibody; autophagosomes; autophagy; blotting; cells; complete; confocal; control; coronavirus; corresponding; data; degradation; effect; encephalomyelitis; et al; experiments; figure; fluorescence; flux; formation; fusion; gfp; hpi; infected; infection; inhibitor; lamp1; lc3; lysosomal; lysosomes; mechanism; membrane; microscopy; min; mock; nerve; neuro-2a; neuro-2a cells; p62; phev; phev infection; phev replication; positive; post; process; protein; ptflc3; puncta; rapamycin; red; replication; results; study; time; treatment; usa; vesicles; viral; virus; viruses; western
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        item: #21 of 41
          id: cord-275307-d7htyfcl
      author: Gaglia, Marta Maria
       title: Transcriptome-Wide Cleavage Site Mapping on Cellular mRNAs Reveals Features Underlying Sequence-Specific Cleavage by the Viral Ribonuclease SOX
        date: 2015-12-08
       words: 10873
      flesch: 53
     summary: In this study, we address this paradox by using high-throughput sequencing of cleavage intermediates combined with a custom bioinformatics-based analysis pipeline to identify SOX cleavage sites across the mRNA transcriptome. To dissect the specificity of SOX cleavage across the mRNA transcriptome, we designed an approach to map SOX cleavage sites in endogenous mRNAs using PARE
    keywords: additional; analysis; annotated; approach; cells; cellular; cleavage; cleavage sites; coding; confidence; confidence level; construct; control; counts; cut; cut sites; cuts; data; degenerate; degradation; different; dimer; distribution; efficiency; element; endogenous; ends; ensembl; experimental; expression; factor; features; fig; figs; fold; fragments; frequency; gene; gfp; gfp mrna; herpesvirus; high; host; human; insertion; kshv; level; likelihood; limd1; location; log; match; motif; mrna; multiplicative; non; nucleotides; number; pare; peaks; position; presence; protein; putative; pwm; read; region; relative; replicates; reporter; results; rna; rnas; samples; scanning; scores; sequence; sequencing; shutoff; shxrn1; similar; sites; sox; sox cleavage; sox cut; sox targeting; specific; specific cut; specific peaks; specificity; srsf3; start; structure; studies; table; targeting; targets; test; transcriptome; transcripts; unpaired; viral; virus; window; xrn1
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        item: #22 of 41
          id: cord-280454-etf32afd
      author: Moustaqil, Mehdi
       title: SARS-CoV-2 proteases cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species and the search for reservoir hosts
        date: 2020-06-05
       words: 10177
      flesch: 51
     summary: (*) the protein sequences for Cotton rats (Sigmodon hispidus) and Minks (Neovison vison) were found using tblastn against shotgun genomes, with the Query AAH7172.1 for IRF3 protein [(Isoform 1) Homo sapiens] and NP_653288.1 for NACHT, LRR and PYD domains-containing protein 12 (C) Schematic representation of TAB1 protein structure with the location of the identified cleavage sites on the primary sequence.
    keywords: 293t; 3clpro; ace2; activation; activity; acute; amino; analysis; anti; asc; assay; atp; bats; binding; buffer; cats; cell; cleavage; cleavage sites; cleaved; concentration; conserved; coronavirus; cov-2; cov2; covid-19; dependent; different; disease; domain; expression; family; fig; figure; fluorescent; formation; fragments; free; gel; gels; gfp; hiips; host; human; ifn; immune; immunity; infected; infection; inflammatory; inhibition; inhibits; innate; interactions; interferon; irf3; kda; kinase; length; like; like protease; loop; lte; mers; migration; models; molecular; motif; mouse; nlrp12; nlrp12 cleavage; nlrp3; non; note; nsp3; nsp5; page; papain; pathway; patients; pdb; plpro; position; potential; presence; present; production; protease; proteins; proteolytic; recognition; relevant; reservoir; residue; respiratory; response; responsible; results; sars; screen; sds; second; sequence; severe; signaling; single; sites; size; species; specific; structure; study; supplementary; syndrome; system; tab1; tag; tak1; target; terminal; terminus; type; viral
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        item: #23 of 41
          id: cord-299509-7xjdryoq
      author: Scholte, Florine E. M.
       title: Characterization of Synthetic Chikungunya Viruses Based on the Consensus Sequence of Recent E1-226V Isolates
        date: 2013-08-01
       words: 9731
      flesch: 44
     summary: To examine CHIKV-induced translational shut-off, the synthesis of 35 S-labeled viral and cellular proteins during the course of CHIKV LS3 infection was analyzed by metabolic labeling of infected 293/ACE2 cells with 35 S-Met and 35 S-Cys (Fig. 3C) . CHIKV RNA was isolated from virions using the QIAamp Viral RNA mini kit.
    keywords: 37uc; a226v; ace2; acid; adenosine; aedes; albopictus; alphaviruses; amino; animal; antiviral; assays; bhk-21; cdna; cells; cellular; characteristics; characterization; chikungunya; chikv; chikv ls3; chikv replication; chloroquine; clinical; clones; clusters; comparison; compounds; consensus; control; cpe; cycle; data; days; deaza; detection; differences; effect; egfp; end; experiments; expression; factors; fig; genbank; gene; genomes; gfp; host; important; individual; infected; infection; inhibitor; interactions; isolates; ita07; italy; kinetics; level; lines; ls3; medium; mice; moi; mosquito; mouse; mutation; natural; negative; new; nl10/152; nsp4; outbreaks; p.i; pbs; points; position; positive; post; probe; promoter; protein; quasispecies; ra1; recent; reduction; replication; reporter; results; ribavirin; rna; samples; sensitivity; sequence; similar; specific; strains; strand; studies; study; subgenomic; synthesis; synthetic; table; time; titers; total; transcription; uridine; values; vector; vero; virus; viruses
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        item: #24 of 41
          id: cord-307067-cpc1yefj
      author: van Doremalen, Neeltje
       title: A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques
        date: 2020-06-10
       words: 6256
      flesch: 45
     summary: To investigate whether vaccination with ChAdOx1 MERS vaccination provides protection against a variety of different MERS-CoV strains, we vaccinated BALB/c mice transgenic for hDPP4 with ChAdOx1 MERS or ChAdOx1 GFP 28 days before challenge with 10 4 TCID 50 of one of six diverse MERS-CoV strains ( fig. In the current manuscript, we show that a single dose of ChAdOx1 MERS vaccine protects rhesus macaque model against a mucosal challenge with HCoV-EMC/2012.
    keywords: adenovirus; africa; alveolar; animals; antibodies; antibody; assay; bal; boost; bronchointerstitial; camels; cells; chadox1; chadox1 gfp; chadox1 mers; challenge; clinical; control; coronavirus; cov; days; different; disease; dose; dpi; dromedary; east; edema; emc/2012; fig; gfp; group; hcov; hdpp4; human; immunity; infectious; kit; lesions; limited; lobes; lower; lung; macaques; mean; mers; mice; middle; moderate; mrna; neutralizing; outbreak; pneumonia; presence; prime; protection; protein; pulmonary; reduced; regimen; replication; respiratory; rhesus; serum; significant; signs; single; specific; strains; studies; study; syndrome; tcid; test; time; tissue; titer; transgenic; type; vaccinated; vaccination; vaccine; variety; viral; virus; weight; −28; −56
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        item: #25 of 41
          id: cord-315483-l6dm82pp
      author: Santhakumar, Diwakar
       title: Chicken Interferon-induced Protein with Tetratricopeptide Repeats 5 Antagonizes Replication of RNA Viruses
        date: 2018-05-01
       words: 10827
      flesch: 39
     summary: The second exon codes the rest of the ORF (1435 bps) and 3′ untranslated regions (UTR) for chicken IFIT5 gene. Chicken cells were transfected with 500 ng of mammalian expression vectors encoding V5-tagged chIFIT5 for 24 hours and were left untreated (NDV-) or were treated with 1 MOI of NDV-GFP (NDV+) for another 24 hours before fixation, staining for nucleus (blue), chIFIT5 (red) and GFP marker (NDV).
    keywords: 101/+123; activation; activities; activity; analysis; antibodies; antiviral; assay; cas9; cefs; cells; cellular; characterized; chicken; chifit5; chifit5 gene; chifn; constructs; control; crispr; data; df-1; df-1 cells; different; disease; dna; dsrna; editing; effect; elements; embryos; endogenous; ensembl; expression; facs; family; ffluc; fibroblasts; fig; functional; gene; genetic; genome; genomic; gfp; host; hours; huifit5; human; ifit1; ifn; ifns; immune; indicated; individual; induction; infected; infection; influenza; innate; interaction; interferon; invitrogen; isre; lentivirus; locus; luciferase; mammalian; mice; mock; motifs; mouse; mrna; ndv; negative; non; orf; ovo; pcr; plasmid; positive; post; potential; ppp; presence; primers; products; profound; promoter; protein; puromycin; recognition; region; replication; reporter; reports; responses; results; retroviruses; rna; sense; sequence; shrna; silencing; single; sites; species; specific; stimulation; stimuli; structure; supplementary; system; table; tetratricopeptide; time; total; transcription; transfected; transgenic; translation; type; usa; vector; virus; virus replication; viruses; vsv
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        item: #26 of 41
          id: cord-316814-9fv9xrln
      author: Li, Hong-Ye
       title: Use of GFP to Investigate Expression of Plant-Derived Vaccines
        date: 2009
       words: 2822
      flesch: 49
     summary: Results of western blot analysis using antibodies against GFP of total plant proteins extracted from tobacco leaves infiltrated with plasmid pCV12 (expressing the S1:GFP fusion) are shown in Fig. The GFP tag enables a relatively quick confirmation of antigen expression in plant cells by fluorescent microscopy.
    keywords: agrobacterium; agroinfiltration; analysis; blot; buffer; cdna; cells; cov; dna; expression; fig; fluorescent; fusion; gel; gene; gfp; leaf; leaves; medium; membrane; microscopy; min; nuclear; optimal; oral; plant; plasmid; protein; recombinant; sars; subcellular; tobacco; transfer; transformation; transient; use; vaccines; vector; viral
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        item: #27 of 41
          id: cord-317142-qd61qvch
      author: Muramatsu, Tomonari
       title: Autoprocessing mechanism of severe acute respiratory syndrome coronavirus 3C‐like protease (SARS‐CoV 3CL (pro)) from its polyproteins
        date: 2013-03-27
       words: 6370
      flesch: 53
     summary: They used two types of pro-3CL pro molecules with an N-terminal pro-sequence of six amino acids, accompanied by CFP and YFP at the N-and C-termini, respectively: one contains an active (wild-type) protease site and and inactive N-terminal processing site (Q-1E) (enzyme), and the other contains an inactive protease site (C145A) and an active (wild-type) N-terminal processing site (substrate). This assumption is supported by the difference in the inhibitory effects of the N-terminal pro-sequences of the two substrates, the pro-3CL(C145A) and GFP substrate, on the N-terminally processed enzyme/proenzyme (WT or Q306N; Fig. 4 ).
    keywords: 3cl pro; acid; active; activities; activity; amino; autoprocessing; c145a; calculated; cat; cell; cleavage; coli; coronavirus; cov; dimer; enzyme; fig; form; free; gfp; gfp substrate; mature; mature form; molecular; molecule; page; polyprotein; pro; pro pro; pro-3cl(c145a; processing site; protease; protein; protomer; q-1n; q306n; reaction; respiratory; sars; sds; sequence; site; species; substrate; syndrome; synthesis; system; tag; terminal pro; terminal processing; terminus; trans; values
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        item: #28 of 41
          id: cord-319517-denczc6t
      author: Salipalli, Sandeep
       title: Recent advances in live cell imaging of hepatoma cells
        date: 2014-07-08
       words: 9219
      flesch: 41
     summary: An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore Evolutionary optimization of fluorescent proteins for intracellular FRET Fluorescent proteins from nonbioluminescent Anthozoa species A monomeric red fluorescent protein Dynamic motion of paxillin on actin filaments in living endothelial cells A guide to choosing fluorescent proteins Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein Bright monomeric red fluorescent protein with an extended fluorescence lifetime Green fluorescent protein as a marker for gene expression Aequorea green fluorescent protein: expression of the gene and fluorescence characteristics of the recombinant protein Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging In vitro activity of fluorescent dyes against asexual blood stages of Plasmodium falciparum Antibodies for fluorescent molecular rotors ReAsH/FlAsH labeling and image analysis of tetracysteine sensor proteins in cells Specific covalent labeling of recombinant protein molecules inside live cells Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications Fluorogen-activating single-chain antibodies for imaging cell surface proteins Moreover, ELuc was tested in cell lines such as NIH/3T3 using various promoters, and it was shown that the luciferase primarily localizes in nucleus, cytosol and in peroxisomes.
    keywords: acid; activity; adrp; analysis; assays; atp; bacteria; blue; cationic; cell; cell imaging; cell lines; cfp; color; complex; cytotoxicity; delivery; development; different; dna; droplets; dyes; ebfp; ecfp; edt2; efficiency; egfp; emission; energy; enzyme; excitation; experiments; expression; factors; faps; figure; fluc; fluorescent; fluorescent protein; fold; formation; fps; fret; gene; gene transfer; gfp; green; green fluorescent; hepatocytes; hepatoma; hepg2; high; higher; host; human; imaging; improved; increase; intensity; interaction; intracellular; light; lines; lipid; lipid droplets; lipofection; live; living; localization; long; luciferase; major; mammalian; marker; maturation; mdh; membrane; methods; mice; molecular; molecules; mouse; mutants; mutations; non; oil; organelles; photostability; photostable; plasmid; plin2; promoters; properties; protein; range; recent; red; reporter; rfp; rna; signal; single; specific; spectra; stable; staining; structure; studies; study; surface; system; target; technique; time; transfection; transfection efficiency; transfer; type; understanding; use; variants; vectors; viral; virus; vivo; wavelength; yellow; yfp
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        item: #29 of 41
          id: cord-319842-4mnaicki
      author: Jackson, William T
       title: Subversion of Cellular Autophagosomal Machinery by RNA Viruses
        date: 2005-04-26
       words: 6959
      flesch: 33
     summary: As can be seen in Figure 1 , the punctate GFP-LC3 signal colocalized with that of poliovirus RNA replication protein 3A, as visualized by immunofluorescence. It is thought that one function for membrane localization of viral RNA replication proteins is to promote their oligomerization [6, 7] .
    keywords: 2bc; analysis; antibody; atg12p; autophagosome; autophagy; bacterial; california; cells; cellular; colocalization; complex; complexes; components; contents; copii; cytoplasmic; cytosol; cytosolic; double; early; electron; endoplasmic; endosomes; expression; facilitate; figure; fixation; fold; formation; fusion; gfp; golgi; hela; host; human; induced; induction; infected; infection; inhibition; intracellular; lamp1; late; lc3; like; lysis; machinery; mammalian; maturation; mature; mdc; mechanism; membraned; membranous; microscopy; murine; nonlytic; origin; particles; pathway; pbs; poliovirus; poliovirus infection; poliovirus proteins; poliovirus rna; positive; presence; protein; punctate; rapamycin; release; replication; response; reticulum; rhinovirus; rna; rna replication; role; sirna; specific; staining; states; structures; tamoxifen; target; treatment; type; united; vesicles; viral; virions; viruses; wtj; yield
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        item: #30 of 41
          id: cord-324720-acei0pn8
      author: Howe, Charles L
       title: Inflammatory monocytes damage the hippocampus during acute picornavirus infection of the brain
        date: 2012-03-09
       words: 5542
      flesch: 47
     summary: Uninfected mice, untreated Theiler's murine encephalomyelitis virus (TMEV)-infected mice (No Tx), and infected mice treated with RB6-8C5 to deplete inflammatory monocytes and neutrophils (RB6 Tx) were assessed in the Morris water maze starting at 14 dpi. Phenotypic differences defined and direct ex vivo antigen presentation to myelin basic protein-reactive CD4+ T cells compared Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response Characterization of the five novel Ly-6 superfamily members encoded in the MHC, and detection of cells expressing their potential ligands Inflammatory monocytes damage the hippocampus during acute picornavirus infection of the brain
    keywords: + +; 1a8; acute; antigen; bils; biosciences; brain; cd11b; cd45; cells; clone; cns; cognitive; contrast; control; depletion; encephalomyelitis; experiments; f4/80; figure; findings; flow; following; gfp; gr1; hippocampal; hpi; immune; infected; infection; infiltrate; infiltrating; inflammatory; inflammatory monocytes; injury; leukocytes; ly6; ly6c; macrophages; maze; mice; monocytes; murine; neutrophils; pathology; population; positive; present; rb6; response; sham; staining; surface; system; theiler; tmev; viral; virus; water
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        item: #31 of 41
          id: cord-330176-1ugzkf22
      author: Weeratunga, Prasanna
       title: RETRACTED ARTICLE: Interferon-mediated antiviral activities of Angelica tenuissima Nakai and its active components
        date: 2016-01-05
       words: 7988
      flesch: 45
     summary: These results indicate that oral inoculation of Angelica tenuissima Nakai extracts stimulate mucosal immune cells of small intestine and ultimately induce the strong inhibition of influenza virus replication in bronchoalveolar through the mucosal immune response system. Of 11 fractions, FR-5 was detected at a wavelength and retention time of 325 nm and 16.33 min, and the concentration of FR-5 in 6.0 mg/ml (w/v) of Angelica tenuissima Nakai extract was found to be 5.48 μg/ml ( Fig. 7A and B) .
    keywords: acid; activities; activity; angelica; angelica tenuissima; antiviral; aqueous; assay; balb; balf; body; cells; compounds; control; culture; cytokines; diseases; dmem; dpi; drugs; effects; elisa; ev-71; expression; extract; ferulic; fig; fold; fractions; gfp; groups; hek293; hela; higher; hpi; hpt; hsv; human; ifn; ifns; iga; il-6; immune; induction; infected; infection; inflammatory; influenza; interferon; levels; lung; mice; min; murine; nakai; pbs; plant; plates; pr8; pro; raw264.7; raw264.7 cells; reduced; reduction; replication; results; secretion; signaling; significant; state; study; survival; system; tenuissima; tenuissima nakai; tested; time; titers; total; treatment; type; untreated; virus; viruses; vitro; vivo; vsv; weight
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        item: #32 of 41
          id: cord-331414-i0oxm5mr
      author: Kautz, Tiffany F.
       title: A Low Fidelity Virus Shows Increased Recombination during the Removal of an Alphavirus Reporter Gene
        date: 2020-06-19
       words: 5071
      flesch: 40
     summary: RNA virus recombination primarily occurs when the RdRp switches from the template RNA strand to an acceptor RNA strand (i.e., copy choice recombination) or, less frequently, by ligation of cleaved RNA strands [8] . key: cord-331414-i0oxm5mr authors: Kautz, Tiffany F.; Jaworski, Elizabeth; Routh, Andrew; Forrester, Naomi L. title: A Low Fidelity Virus Shows Increased Recombination during the Removal of an Alphavirus Reporter Gene date: 2020-06-19 journal: Viruses DOI: 10.3390/v12060660 sha: doc_id: 331414 cord_uid: i0oxm5mr Reporter genes for RNA viruses are well-known to be unstable due to putative RNA recombination events that excise inserted nucleic acids.
    keywords: alphavirus; analysis; beginning; cells; clickseq; common; defective; deletions; encephalitis; end; equine; events; evolution; fidelity; fidelity tc-83; figure; fluorescent; fold; frequency; gene; genome; gfp; gfp gene; high; higher; identical; infection; low; mutant; nucleotide; number; observed; passage; passaging; plaques; polymerase; population; rates; rdrp; reads; recombination; recombination events; removal; replicates; replication; reporter; rna; rna recombination; sequence; sequencing; sindbis; small; stability; structure; tc-83; tc-83 gfp; usa; vaccine; vero; viral; virus; viruses; yellow
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        item: #33 of 41
          id: cord-332881-mkm4ygh6
      author: Kirchhoff, Jana
       title: Three viruses of the bovine respiratory disease complex apply different strategies to initiate infection
        date: 2014-02-18
       words: 6411
      flesch: 46
     summary: Respiratory epithelial cells were also refractory to infection by BRSV. Another culture system for differentiated respiratory epithelial cells are precision-cut lung slices (PCLS), where the epithelial cells are maintained in their original setting.
    keywords: additional; airway; ali; apical; baec; barrier; basal; basolateral; bhv-1; bovine; bpiv3; brsv; cells; ciliated; complex; contrast; cultures; days; determined; differentiated; disease; efficiency; egta; entry; epithelial; epithelial cells; figure; file; filter; fusion; gfp; hpi; human; incubation; infected; infection; influenza; junctions; medium; membrane; pathogens; pbs; pcls; pneumocytes; polarized; post; primary; protein; purpose; receptor; release; resistance; respiratory; slices; staining; strategies; studies; supernatant; surface; susceptible; syncytial; system; teer; time; tract; ttf-1; type; values; viral; virus; viruses
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        item: #34 of 41
          id: cord-334855-s0ci3r8w
      author: Andersen, Petter I.
       title: Novel Antiviral Activities of Obatoclax, Emetine, Niclosamide, Brequinar, and Homoharringtonine
        date: 2019-10-18
       words: 4295
      flesch: 43
     summary: For testing the production of HSV-2 and EV1 viruses in compound-treated and non-treated RPE cells, the media from the cells were serially (10-fold) diluted, starting from 10 −3 to 10 −8 in serum-free growth media containing 0.2% bovine serum albumin, and applied to a monolayer of A549-Npro cells in 12-well plates. This result indicates that obatoclax and emetine target distinct cellular pathways essential for virus infection.
    keywords: activities; activity; addition; agents; antiviral; assay; bovine; brequinar; broad; bsaas; cells; cellular; compounds; concentrations; control; ctg; death; different; diseases; dmem; drug; emetine; ev1; expression; fbs; figure; fluav; fluorescent; gfp; glutamine; hmpv; homoharringtonine; hsv-2; human; infected; infection; inhibits; initial; luciferase; medium; mock; niclosamide; non; novel; obatoclax; pen; production; protein; replication; rfp; rpe; rpe cells; rvfv; safe; serum; spectrum; strain; strep; table; type; viability; virus; viruses; vitro
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        item: #35 of 41
          id: cord-338811-2bi2edcw
      author: Lennemann, Nicholas J.
       title: Imaging-Based Reporter Systems to Define CVB-Induced Membrane Remodeling in Living Cells
        date: 2020-09-25
       words: 6682
      flesch: 38
     summary: Live-cell imaging of CVB infected cells The data was collected from two independent experiments, with four separate fields/experiment. Live-cell imaging of CVB infected cells expressing these reporters allowed for the real-time visualization of virus-induced changes to the host cell, including the collapse of the peripheral ER network and loss of Golgi integrity.
    keywords: 2apb; analysis; area; calcium; cells; changes; cleavage; collapse; cvb; cytoplasmic; decrease; dispersal; endoplasmic; enterovirus; expressing; figure; fluorescent; gfp; golgi; host; hpi; image; imaging; increase; infected; infection; inhibitor; integrity; intensity; kinetics; lapse; live; long; manipulation; mcherry; membrane; nls; nuclear; nucleus; organelles; pathway; peripheral; plasmid; poliovirus; previous; protease; protein; ratio; real; region; release; remodeling; reper; replication; reporter; results; reticulum; rna; secretory; series; signal; strand; structures; system; term; time; translocation; u2os; usa; video; viral; virus; viruses
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        item: #36 of 41
          id: cord-342189-ya05m58o
      author: Banerjee, Abhik K.
       title: SARS-CoV-2 disrupts splicing, translation, and protein trafficking to suppress host defenses
        date: 2020-10-08
       words: 11499
      flesch: 45
     summary: Because viral membrane proteins also require trafficking to the ER, viral disruption of SRP might negatively impact viral propagation, unless viral proteins are trafficked in an SRP-independent manner ( Figure S7E ) or if NSP8/9 selectively impacts host (but not viral) proteins. Understanding the interactions between viral proteins and components of human cells is essential for elucidating their pathogenic mechanisms and for development of effective therapeutics.
    keywords: 18s; 40s; 5eu; 7sl; addition; analysis; anti; antiviral; assay; binding; buffer; cells; cellular; channel; complex; components; control; coronavirus; cov-2; covid-19; critical; dependent; disruption; elution; entry; essential; et al; expression; figure; fisher; flow; fluorescence; fold; functions; gene; gfp; global; hek293; hepes; host; hours; human; ifn; immune; impact; infected; infection; inhibition; initiation; innate; input; interaction; interferon; intron; j o; kit; leader; levels; mammalian; manufacturer; mechanisms; media; membrane; min; minutes; model; molecular; mrna; nascent; ngfr; non; nsp1; nsp8; nsp9; nucleotide; number; observed; particle; pdb; presence; processes; protein; purified; puromycin; r n; ratio; reactions; reads; recognition; reduction; region; relative; replication; reporter; response; results; ribosomal; ribosome; rna; rnas; roles; rrna; sample; sars; scientific; sequence; signal; site; sl1; small; specific; splice; splicing; srp; strong; structural; subunit; sunset; suppression; surface; system; table; test; thermo; total; trafficking; transcribed; transcription; translation; type; unspliced; vectors; viral; viral proteins; viruses; washed; window
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        item: #37 of 41
          id: cord-345651-admlzeu4
      author: Wang, Gang
       title: The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets
        date: 2019-03-30
       words: 6244
      flesch: 40
     summary: The 43.5-kilodalton band of TGEV N protein ( Figure 2C ) and the marker mutation at position 4553 in the TGEV genome ( Figures 1B and 2D ) confirmed the recovery of the TGEV-GFP recombinant virus. The 43.5-kilodalton band of TGEV N protein ( Figure 2C ) and the marker mutation at position 4553 in the TGEV genome ( Figures 1B and 2D ) confirmed the recovery of the TGEV-GFP recombinant virus.
    keywords: bac; bacterial; cas9; cdna; cells; china; clone; coronavirus; corresponding; cov; covs; days; deletion; determinant; digested; domain; editing; enteric; epidemic; figure; fragment; gastroenteritis; gene; genetics; genome; gfp; gfp bac; gfp-∆s_ntd; group; infectious; intestinal; length; mock; mutation; pcr; piglets; porcine; post; primers; products; protein; recombinant; recombination; respiratory; reverse; rna; s_ntd224; sequence; sequencing; severe; sgrna; sites; specific; spike; study; syndrome; table; targeted; terminal; terminus; tgev; tissue; transmissible; tropism; usa; vector; virulence; virus; viruses
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        item: #38 of 41
          id: cord-346554-a98pjtxs
      author: Uddin, Md Bashir
       title: Inhibitory effects of bee venom and its components against viruses in vitro and in vivo
        date: 2016-11-26
       words: 7918
      flesch: 48
     summary: BV or MLT antiviral activity was tested in HeLa cells with H3-GFP and EV-71 viruses using standard antiviral activity assays. Additionally, BV or MLT antiviral activity for EV-71 was determined by evaluating 50% tissue culture infective dose (TCID 50 ) and analysis of mRNA expression levels of the EV-71 VP1 gene.
    keywords: activity; antiviral; assay; bars; bee; broad; cells; components; control; data; different; dmem; dpi; effect; enveloped; ev-71; experiments; expression; fbs; fig; fold; fractions; gene; gfp; gfp expression; gradient; groups; h1n1; hek293; hela; hpi; hsv; human; ifn; incubated; incubation; infected; infection; infectivity; influenza; kim; levels; mdck; mechanism; medium; melittin; membranes; mice; min; mlt; non; particles; pbs; peptide; plates; post; pr8; properties; replication; rsv; significant; spectrum; study; sucrose; tested; titer; treatment; type; venom; vero; virucidal; virus; viruses; vitro; vp1; vsv; weight
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        item: #39 of 41
          id: cord-348799-qu4zin3o
      author: Wu, Nannan
       title: The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation
        date: 2019-10-10
       words: 11434
      flesch: 42
     summary: B) Control cells were analyzed by confocal microscopy with the endogenous P bodies markers TNRC6A and DDX6 (ISG20-expressing cells and a portion of the overlay of control cells depicted here are presented in Fig 5A) . Translationcompetent mRNA generated in vitro were transfected in ISG20 expressing cells, prior to cell lysis one hour afterwards and analyses by RT-qPCR or luciferase assays.
    keywords: ability; absence; accumulation; activities; activity; analysis; antiviral; assay; associated; bodies; buffer; case; cells; cellular; coding; cold; complete; conditions; confocal; control; cytoplasmic; degradation; different; dna; dnas; dox; ebv; ectopic; effects; elements; exonuclease; experiments; expression; factor; fig; flag; following; fractions; functions; gag; gene; genetic; genome; gfp; graph; hek293; hek293 t; hela; hepatitis; host; hours; human; ifit1; ifn; important; inactive; independent; indicated; induced; infection; inhibition; initiation; interferon; invitrogen; isg20; levels; lines; luciferase; lyon; material; mechanism; medium; mice; microscopy; minutes; mrnas; murine; mutants; myc; non; nuclear; origins; overall; p bodies; panels; presence; present; prior; production; properties; proportion; protein; qpcr; replication; reporter; results; ribosomal; ribosomes; rluc; rna; role; self; sigma; similar; single; specific; stable; student; study; supernatant; system; t cells; target; test; tnrc6a; transfection; translation; type; typical; u937; vectors; viral; virus; viruses; vitro; vivo; vsv
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        item: #40 of 41
          id: cord-353467-wbtzvm4i
      author: Lambert, Carsten
       title: Functional incorporation of green fluorescent protein into hepatitis B virus envelope particles
        date: 2004-12-05
       words: 6333
      flesch: 42
     summary: Among the three related large L, middle M, and small S envelope proteins, L has been shown to play the key role in receptor recognition and cell attachment (Le Seyec et al., 1999; Neurath et al., 1986) . Because HBV empty envelope particles, built from the S protein, provide a safe antigen delivery system, their use as a carrier for the presentation of various antigens is a long established practice.
    keywords: absence; addition; analysis; antibodies; antibody; antigen; assembly; attachment; binding; cells; centrifugation; chains; contrast; data; density; detection; dna; domain; envelope; et al; expression; fig; fluorescent; formation; forms; fractions; fusion; genome; gfp; gfp.s; glycosylated; gradient; green; hbsag; hbv; hepatitis; hepatocytes; hepg2; human; incorporation; infection; intracellular; large; mab; membrane; microsomes; mouse; np-40; particles; plasmid; presence; protein; proteinase; receptor; s.yfp; secretion; sha; site; specific; structure; subviral; sucrose; supernatants; surface; tag; terminal; terminus; topology; transmembrane; type; viral; virion; virus; western; wild
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        item: #41 of 41
          id: cord-354052-x4ckzw64
      author: Li, Chunhua
       title: Manipulation of the Porcine Epidemic Diarrhea Virus Genome Using Targeted RNA Recombination
        date: 2013-08-02
       words: 4940
      flesch: 46
     summary: siRNA knockdown of ORF3 gene in PEDV infected cells reduced the number of particles released from the cells [5] . Virus Genes Outbreak of porcine epidemic diarrhea in suckling piglets Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in korea PEDV leader sequence and junction sites PEDV ORF3 encodes an ion channel protein and regulates virus production Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus Propagation of the virus of porcine epidemic diarrhea in cell culture Role of proteases in the release of porcine epidemic diarrhea virus from infected cells Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain:
    keywords: assay; bamhi; cdna; cells; coronavirus; culture; deletion; diarrhea; digested; dorf3; epidemic; expression; fig; fragment; fusion; gene; genetics; genome; genomic; gfp; hours; infected; infection; luciferase; mhv; moi; murine; neutralization; orf1b; orf3 gene; orf3 protein; p.i; paci; pcr; pedv; pedv genome; piglets; porcine; positive; primer; protein; recombinant; recombination; renilla; reporter; restriction; reverse; rluc; rna; serum; site; spike; strain; structural; system; targeted; transfer; trypsin; vector; vero; vero cells; viral; virus; viruses; vitro
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