# bib

id: cord-271501-yjobthaj
author: Hirschberg, Carlos B.
date: 1997-02-17
extension: .txt
mime: text/plain
words: 1962
sentence: 51
flesch: 20
cache: cache/cord-271501-yjobthaj.txt
txt: txt/cord-271501-yjobthaj.txt
title:
  Transporters of nucleotide sugars, nucleotide sulfate and ATP in the Golgi apparatus membrane: Where next?

summary:
  Recent evidence in mammals and yeast suggests that Golgi membrane transporters play a regulatory role in determining which macromolecules undergo specific posttranslational modifications in the lumen of the Golgi apparatus (Abeijon et al., 1993; The Golgi guanosine diphosphatase is required for transport of GDP-mannose into the lumen of Saccharomyces cerevisiae Golgi vesicles Effect of nuclcotides on translocation of sugar nucleotides and adenosine 3' phosphate 5' phosphosulfate into Golgi apparatus vesicles Mechanism of phosphorylation in the lumen of the Golgi apparatus: translocation of adenosine 5' triphosphate into Golgi vesicles for rat liver and mammary gland Translocation of ATP Golgi membrane transporters into the lumen of the rough endoplasmic rcticulum-derived vesicles and its binding to lumenal proteins including BiP (GRP 78) and GRP 94 Golgi localization of glycosyltransferases: more questions than answers Expression cloning of the Golgi CMP sialic acid transporter Target sizes of galactosyltransferase, sialyltransferase and uridinediphosphatase in the Golgi apparatus of rat bver Cystine transport is defective in isolated leukocyte lysosomes from patients with cystinosis Targeting of proteins to the Golgi apparatus Topography of glycosylanon in the rough endoplasmic reticulum and Golgi apparatus Mice lacking N-acetylglucosaminyltransferase I activity die at mid-gestation revealing an essential role for complex or hybrid N-lmked carbohydrates Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reuculum Dialectics in carrier research: the ADP/ATP carrier and the uncoupling protein Golgi GDPmannose requires Leishmania LPG2: a member of a eukaryotic family of putative nucleotide-sugar transporter Targeting and retention of Golgi membrane proteins Characterization of a proton-driven carrier for sialic acid in the lysosomal membrane Purification of tbe Golgi adenosine 3' phosphate 5'-phosphosulfate transporter, a homodimer within the membrane A monomeric protein in the Golgi membrane catalyzes both N-deacetylation and N-sulfation of heparan sulfate An ATP transporter it required for protein translocation into the yeast endoplasmic reticulum Saclp mediates the adenosine triphosphate transport into yeast endoplasmic reticulum that is required for protein translocation Complex asparagine-linked oligosaccharides are required for morphogenic events during postimplantation development Reconstitution of Golgi vesicle CMPsialic acid and adenosine 3' phosphate 5' phosphosulfate transport into proteoliposomes Characterization of the Saccharomyces cerevisiae Golgi complex through the cell cycle by immunoelectronmicroscopy Defect in vitamin B 12 metabolism The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca 2+ ATPase family The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the retl-1 mutation in a-COP Transport of sugar nucleotides into rat liver Golgi: a new Golgi marker activity A Golgi retention signal in a membrane-spanning domain of coronavirus El protein Defective lysosomal egress of free sialic acid (Nacetylneuraminic acid) in fibroblasts of patients with infantile free sialic acid storage disease Transport of UDP-Galactose into the Golgi lumen regulates the biosynthesis of proteoglycans The KKXX signal mediates retrieval of membrane proteins from the Golgi to the ER in yeast Eur UDP-GlcNAc transport across the Golgi membrane: electroneutral exchange for dianionic UMP Acknowledgments I thank C. Abeijon, J. Baenziger, M. Milla, and E. Mandon for helpful comments, and Annette Stratton and Karen Welch for excellent secretarial assistance.


id: cord-272467-8heg5iql
author: Armstrong, John
title: Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA
date: 2004-02-19
extension: .txt
mime: text/plain
words: 2756
sentence: 116
flesch: 51
cache: cache/cord-272467-8heg5iql.txt
txt: txt/cord-272467-8heg5iql.txt
summary:
  E l protein was synthesized in oocytes as a spectrum of forms, the most mobile of which comigrated with the unmodified protein from the reticulocyte lysate (Fig. 1 , lanes e,f). In contrast, E l protein showed a much more localised fluorescence pattern (Fig. 2b) .


id: cord-022774-wasdp2gh
author: Morré, D. James
date: 2012-02-07
extension: .txt
mime: text/plain
words: 3916
sentence: 152
flesch: 43
cache: cache/cord-022774-wasdp2gh.txt
txt: txt/cord-022774-wasdp2gh.txt
title:
  Identification of the 16°C compartment of the endoplasmic reticulum in rat liver and cultured hamster kidney cells

summary:
  Many of the vesicles re- mained attached to larger endoplasmic reticulum vesicles by a narrow stalk (Fig. 1) . Overall, the numbers of transition vesicles present at the cis Golgi apparatus face showed an increase with increasing temperature to an optimum at 16°C (30 rain) or 18°C (60 rain) with an abrupt decline in transition vesicle numbers between 18°C and 20°C (Fig. 5) .


id: cord-296416-q0rsfzgw
author: LAVI, EHUD
date: 1996-07-15
extension: .txt
mime: text/plain
words: 4779
sentence: 183
flesch: 45
cache: cache/cord-296416-q0rsfzgw.txt
txt: txt/cord-296416-q0rsfzgw.txt
title:
  Syncytia Formation Induced by Coronavirus Infection Is Associated with Fragmentation and Rearrangement of the Golgi Apparatus

summary:
  However, when the BFA treatment began 12 hr or more after viral infection cells had diffuse staining of the GA when observed 8 hr postinfection as did uninfected cells inoculation, there were relatively minimal effects on syncytia formation, and viral titers were 1-2 logs lower as following a similar treatment with BFA. The kinetics of To further investigate the morphologic aspects of the the microtubule changes after infection with MHV-A59 fragmented GA in syncytia of L2 cells infected with MHVwas depicted by immunofluorescence on L-2 cells A59, an electron microscopic examination was perafter infection with MHV-A59 (m.o.i. Å 1 PFU/cell) and formed in cells 24 hr after infection and at a multiplicity after staining with anti-tubulin antibodies and FITCof infection (m.o.i.) of 1 plaque forming unit per cell.


id: cord-352854-che3iwu3
author: Hart, Kristen C
date: 1997-12-18
extension: .txt
mime: text/plain
words: 5995
sentence: 227
flesch: 52
cache: cache/cord-352854-che3iwu3.txt
txt: txt/cord-352854-che3iwu3.txt
title:
  Derivatives of activated H-ras lacking C-terminal lipid modifications retain transforming ability if targeted to the correct subcellular location

summary:
  Post-translational modi®cations of ras proteins occur at the C-terminus, which includes a domain known as the`CAAX' box, where A is an aliphatic amino acid, and X represents any amino acid. The palmitoylation reactions are reversible, allowing for potentially dynamic localization of ras proteins (Magee et al., 1987) .


id: cord-005034-wyipzwo4
author: Gleeson, Paul A.
title: Targeting of proteins to the Golgi apparatus
date: 1994
extension: .txt
mime: text/plain
words: 6551
sentence: 307
flesch: 45
cache: cache/cord-005034-wyipzwo4.txt
txt: txt/cord-005034-wyipzwo4.txt
summary:
  There are a number of distinct groups of Golgi membrane proteins, including glycosyltransferases, recyclingtrans-Golgi network proteins, peripheral membrane proteins, receptors and viral glycoproteins. Interestingly no soluble resident Golgi proteins have been identified within the lumen of the Golgi apparatus, which probably indicates that the mechanisms for retaining proteins to this organelle are restricted to membraneassociated proteins.


id: cord-104223-ht3ry9i0
title: Sorting within the regulated secretory pathway occurs in the trans- Golgi network
date: 1990-01-01
extension: .txt
mime: text/plain
words: 6524
sentence: 223
flesch: 44
cache: cache/cord-104223-ht3ry9i0.txt
txt: txt/cord-104223-ht3ry9i0.txt
summary:
  The flow of the amino-terminal intermediate from small to large immature vesicles could arise by (a) distinct aminoterminal specific small vesicles increasing in size or (b) movement of the amino-terminal intermediate from small immature vesicles containing carboxy-terminal intermediates to a distinct class of large immature vesicles. The above results suggest that sorting occurs as the aminoterminal intermediate moves from small immature vesicles and clear vesicles to large immature vesicles, all of which are defined by acid phosphatase cytochemistry as part of the TGN.


id: cord-003761-ikni2acz
author: Li, Zengbin
date: 2019-06-04
extension: .txt
mime: text/plain
words: 6439
sentence: 258
flesch: 34
cache: cache/cord-003761-ikni2acz.txt
txt: txt/cord-003761-ikni2acz.txt
title:
  Biological Function and Application of Picornaviral 2B Protein: A New Target for Antiviral Drug Development

summary:
  In this review, we mainly summarize recent research data on the viroporin or viroporin-like activity of 2B proteins, which affects the biological function of the membrane, regulates cell death, and affects the host immune response. In addition, the 2B protein exhibits a viroporin or viroporin-like activity, and thus, targeted drugs against viroporin could potentially target 2B protein as a novel strategy to treat or prevent picornavirus infections.


id: cord-007353-qg2pb884
author: Lavi, Ehud
title: Polarity of processes with Golgi apparatus in a subpopulation of type I astrocytes
date: 1994-06-06
extension: .txt
mime: text/plain
words: 6278
sentence: 227
flesch: 38
cache: cache/cord-007353-qg2pb884.txt
txt: txt/cord-007353-qg2pb884.txt
summary:
  In contrast to type I astrocytes, in type II astrocytes there was no evidence for extensions of the GA into cell processes. Since cultures were not 100% pure for any cell type we wished to further confirm that GA in cell processes was present only in type I astrocytes.


id: cord-007261-b5fgb9wf
author: Murakami, Kazuya
date: 1994-07-17
extension: .txt
mime: text/plain
words: 6995
sentence: 212
flesch: 43
cache: cache/cord-007261-b5fgb9wf.txt
txt: txt/cord-007261-b5fgb9wf.txt
title:
  The Transmembrane Region of Microsomal Cytochrome P450 Identified as the Endoplasmic Reticulum Retention Signal(1)

summary:
  The N-terminal transmembrane region of microsomal P-4508 and the transmembrane region of Golgi membrane proteins comprise similar hydrophobic stretches, but the former retains the proteins in the ER whereas the latter keeps the proteins in the Golgi membrane. Recently, Szczesna-Skorupa and Kemper (34) reported that P450 2C2, which was localized in the ER when expressed in COS cells, did not show any shift in intracellular distribution when the cells were incubated at low temperatures, at which some membrane bound ER proteins have been shown to shift to the intermediate or cis-Golgi apparatus.


id: cord-317070-awip52k7
date: 1993-07-01
extension: .txt
mime: text/plain
words: 7401
sentence: 273
flesch: 45
cache: cache/cord-317070-awip52k7.txt
txt: txt/cord-317070-awip52k7.txt
title:
  Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex

summary:
  Alignment of protein sequences was achieved with the GAP program that used a published algorithm (49) . Further support for the authenticity of the cloned cDNA as coding for Golgi complex antigens was obtained by analyzing rabbit antisera raised against SYll, SY10, and SY2 recombinant proteins.


id: cord-292688-w4zvfkyl
author: Tooze, Sharon A
title: Biogenesis of secretory granules in the trans-Golgi network of neuroendocrine and endocrine cells
date: 1998-08-14
extension: .txt
mime: text/plain
words: 8178
sentence: 257
flesch: 39
cache: cache/cord-292688-w4zvfkyl.txt
txt: txt/cord-292688-w4zvfkyl.txt
summary:
  Helper proteins for neuroendocrine secretion Chromogranin B (secretogranin I) promotes sorting to the regulated secretory pathway of processing intermediates derived from a peptide hormone precursor The primary structure of human secretogranin II, a widespread tyrosine-sulfated secretory granule protein that exhibits low pH-and calcium-induced aggregation Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network Calcium-and pHdependent aggregation and membrane association of the precursor of the prohormone convertase PC2 Secretory granule content proteins and the luminal domains of granule membrane proteins aggregate in vitro at mildly acidic pH Conversion of proinsulin to insulin occurs coordinately with acidi¢cation of maturing secretory vesicles Clathrin-coated vesicular transport of secretory proteins during the formation of ACTH-containing secretory granules in AtT20 cells Chloroquine diverts ACTH from a regulated to a constitutive secretory pathway in AtT-20 cells However, in the absence of experiments to prove whether secretory proteins are also conveyed by this pathway, the question remains an open one.


id: cord-276358-so390gp4
author: Nieto-Torres, Jose L.
date: 2015-11-30
extension: .txt
mime: text/plain
words: 7188
sentence: 240
flesch: 37
cache: cache/cord-276358-so390gp4.txt
txt: txt/cord-276358-so390gp4.txt
title:
  Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome

summary:
  A virus lacking M2 protein as a live attenuated vaccine Recombinant respiratory syncytial virus bearing a deletion of either the NS2 or SH gene is attenuated in chimpanzees Hexamethylene amiloride blocks E protein ion channels and inhibits coronavirus replication SARS coronavirus E protein forms cationselective ion channels A 193-amino acid fragment of the SARS coronavirus S protein efficiently binds angiotensinconverting enzyme 2 Intracellular proton conductance of the hepatitis C virus p7 protein and its contribution to infectious virus production Participation of c-FLIP in NLRP3 and AIM2 inflammasome activation Molecular physiology of the SERCA and SPCA pumps Severe acute respiratory syndrome coronavirus group-specific open reading frames encode nonessential functions for replication in cell cultures and mice Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia Viral calciomics: interplays between Ca 2 þ and virus The work done by the authors was supported by Grants from the Government of Spain (BIO2013-42869-R, FIS2013-40473-P), Generalitat Valenciana (Prometeo 2012/069), Fundació Caixa Castelló-Bancaixa (P1-1B2012-03) and a U.S. National Institutes of Health (NIH) project (5P01 AI060699). Therefore, E protein channel is equally selective for Cl À and Ca 2 þ in neutral membranes.


id: cord-293038-pjjvfdnq
author: Fontana, Juan
title: The unique architecture of Bunyamwera virus factories around the Golgi complex
date: 2008-06-10
extension: .txt
mime: text/plain
words: 7398
sentence: 297
flesch: 45
cache: cache/cord-293038-pjjvfdnq.txt
txt: txt/cord-293038-pjjvfdnq.txt
summary:
  Interestingly, integrity of Golgi actin and viral NSm protein is also important for viral tube structure and normal production of infectious virions. In the present work we have studied a large number of serial sections and discovered that viral tubes actually contain a tubular domain and a bigger globular domain on one of the extremities (Fig. 1L ).


id: cord-008590-xivnsldf
author: Tartakoff, Alan M.
date: 2008-04-14
extension: .txt
mime: text/plain
words: 8151
sentence: 333
flesch: 44
cache: cache/cord-008590-xivnsldf.txt
txt: txt/cord-008590-xivnsldf.txt
title:
  The Confined Function Model of the Golgi Complex: Center for Ordered Processing of Biosynthetic Products of the Rough Endoplasmic Reticulum

summary:
  In other cells and employing somewhat different protocols the major intra-Golgi site of accumulation of membrane proteins in the presence of monensin may be such as to have allowed somewhat further oligosaccharide maturation (Table I) . Secretory cells may be divided into two classes: the nonregulated class (e.g., plasma cells, fibroblasts) and the regulated class (e.g., pancreatic and pituitary cell types).


id: cord-319626-f1b3ygg0
title: Site of addition of N-acetyl-galactosamine to the E1 glycoprotein of mouse hepatitis virus-A59
date: 1988-05-01
extension: .txt
mime: text/plain
words: 8245
sentence: 287
flesch: 49
cache: cache/cord-319626-f1b3ygg0.txt
txt: txt/cord-319626-f1b3ygg0.txt
summary:
  Orci et al. (1986) have shown by immunocytochemistry that coats of this type associated with Golgi membranes lack clathrin. The amount of E1 in Golgi membranes cannot be estimated because of the catalytic nature of the peroxidase technique but, until very late in the viral infection, it must be lower than that needed to support budding of the virus.


id: cord-104269-9r7rqqqk
date: 1994-07-02
extension: .txt
mime: text/plain
words: 8656
sentence: 258
flesch: 37
cache: cache/cord-104269-9r7rqqqk.txt
txt: txt/cord-104269-9r7rqqqk.txt
title:
  Sorting signals in the MHC class II invariant chain cytoplasmic tail and transmembrane region determine trafficking to an endocytic processing compartment

summary:
  We reasoned that fi-TR chimeras would be expressed in sufficient amounts on the cell surface to identify Ii sorting signals using quantitative assays which measure Tf internalization and iron uptake . As there is increasing evidence that the same or closely related signals are recognized by the clathrin-based sorting machinery at the plasma membrane and trans-Golgi (Letourneur and Klausner, 1992 would also allow identification of Ii sorting signals operative along the intracelhlar trafficking pathway.


id: cord-328082-7c7slfbp
date: 1993-03-02
extension: .txt
mime: text/plain
words: 8929
sentence: 280
flesch: 47
cache: cache/cord-328082-7c7slfbp.txt
txt: txt/cord-328082-7c7slfbp.txt
title:
  Effect of caffeine and reduced temperature (20 degrees C) on the organization of the pre-Golgi and the Golgi stack membranes

summary:
  From G minor to G major Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine ATP-coupled transport of Vesicular Stomatitis virus G-protein between the endoplasmic reticulum and the Goigi Mannosidase II and the 135-kD Golgispecific antigen recognized by monoclonal antibody 53FC3 are the same dimeric protein Trimeric G-proteins of the trans-Golgi network are involved in the formation of constitutive secretory vesicles and immature secretory granules Trimeric G proteins and vesicle formation Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus Immunoperoxidase methods for the localization of antigens in cultured cells and tissue sections by electron microscopy Distribution of two distinct Ca2+-ATPase like proteins and their relationships to the agonist-sensitive calcium store in adrenal chromaffin cells A monoclonal antibody against a 135K Golgi membrane protein Adenosin Y,5'-phosphate in biological materials Localization of lower molecular weight GTP-binding proteins to exocytic and endocytic compartments Evidence for the regulation of exocytic transport by protein phosphorylation Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum Dissociation ofa 110-kD peripheral membrane protein from the Golgi apparatus is an early event in Brefeldin A action Caffeine overcomes a restriction point associated with DNA replication, but does not accelerate mitosis t-COP, a 110 kD protein associated with non-clathrin coated vesicles and the Golgi complex, shows homology to/3-adaptin Transport of Vesicular Stomatitis virus glycoprotein in a cell-free extract Brefeldin A causes disassembly of the Oolgi complex and accumulation of secretory proteins in the endoplasmic reticulum Efficient transport of Semliki Forest virus glycoproteins through a Golgi complex morphologically altered by Uukuniemi virus glycoproteins Ca 2+ release from endoplasmic reticulum is mediated by a guanine nucleotide regulatory mechanism Die konstitutiou von brefeldin A The endoplasmic reticulure as a protein-folding compartment Isolation and characterization of temperature-sensitive mutants from Semliki Forest Virus Brefeldin A: insights into the control of membrane traffic and organelle structure Role of microtubules in the organization of the Golgi apparatus Low temperature-induced transport blocks as tools to manipulate membrane traffic Effect of caffeine on intracellular transport of Semliki Forest virus membrane glycoproteins Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Characterization of a 58 kDa cis-Golgi protein in pancreatic exocrine cells Driving the cell cycle: M phase kinase, its partners, and substrates A human homologue of the yeast HDEL receptor Ligand-induee.d redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulure The ERD2 gene determines the specificity of the luminal ER protein retention system Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence of membrane cycling from Golgi to ER Microtubule-dependent retrograde transport of proteins into the ER in the presence of Brefeldin This caffeine-induced effect on the Golgi complex was different from that induced by BFA, since mannosidase II, a Golgi stack marker, remained perinuclearly located and the Golgi stack coat protein, beta-COP, was not detached from Golgi membranes in the presence of 10 mM caffeine at 20 degrees C. Electron microscopic analysis showed that, in the presence of caffeine at 20 degrees C, the morphology of the Golgi stack was altered and accumulation of numerous small vesicles in the Golgi region was observed.


id: cord-277566-j3ehiwn9
author: Verheije, Monique H.
title: Mouse Hepatitis Coronavirus RNA Replication Depends on GBF1-Mediated ARF1 Activation
date: 2008-06-13
extension: .txt
mime: text/plain
words: 8957
sentence: 358
flesch: 44
cache: cache/cord-277566-j3ehiwn9.txt
txt: txt/cord-277566-j3ehiwn9.txt
summary:
  MHV RNA replication was not sensitive to BFA in MDCK cells, which are known to express the BFA-resistant guanine nucleotide exchange factor GBF1. Accordingly, individual knockdown of the Golgi-resident targets of BFA by transfection of small interfering RNAs (siRNAs) showed that GBF1, but not BIG1 or BIG2, was critically involved in MHV RNA replication.


id: cord-001082-sufwsu77
author: Bär, Séverine
date: 2013-09-19
extension: .txt
mime: text/plain
words: 8994
sentence: 376
flesch: 42
cache: cache/cord-001082-sufwsu77.txt
txt: txt/cord-001082-sufwsu77.txt
title:
  Vesicular Transport of Progeny Parvovirus Particles through ER and Golgi Regulates Maturation and Cytolysis

summary:
  For the same amounts of viral genome, virus produced in the latter four cells gave rise to 3 to 4 times less NS1-positive cells and up to 10 fold fewer plaques than virus produced in vesicular egressproficient cells (A9). To further characterize this pathway, we analyzed potential association of progeny particles in MVM infected A9 cells with cellular factors known to be involved in generation/loading of perinuclear vesicles.


id: cord-004521-25t4s7fr
author: Marie, M.
title: Membrane traffic in the secretory pathway: Take the ’A’ train: on fast tracks to the cell surface
date: 2008-08-26
extension: .txt
mime: text/plain
words: 9299
sentence: 304
flesch: 37
cache: cache/cord-004521-25t4s7fr.txt
txt: txt/cord-004521-25t4s7fr.txt
summary:
  Analysis of rotavirus assembly and targeting in human intestinal cells reveals an original raftdependent, Golgi-independent apical targeting pathway Rotavirus assembly: an alternative model that utilizes an atypical trafficking pathway Distinct functions for Arf nucleotide exchange factors at the Golgi complex: GBF1 and BIGs are required for assembly and maintenance of the Golgi stack and TGN, respectively Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking Pathways of protein sorting and membrane traffic between the rough endoplasmic reticulum and the Golgi complex The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function ) b-COP localizes mainly to the cis-Golgi side in exocrine pancreas Vesicular tubular clusters between the ER and Golgi mediate concentration of soluble secretory proteins by exclusion from COPI-coated vesicles Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport ER-to-Golgi transport: form and formation of vesicular and tubular carriers Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway Distribution of the intermediate elements operating in ER to Golgi transport Brefeldin A induced dose-dependent changes to Golgi structure and function in the rat exocrine pancreas Maintenance of Golgi structure and function depends on the integrity of ER export Molecular basis for Golgi maintenance and biogenesis Retrograde transport from the pre-Golgi intermediate compartment and the Golgi complex is affected by the vacuolar H+-ATPase inhibitor bafilomycin A1 Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery Evidence for a COPI-independent transport route from the Golgi complex to the endoplasmic reticulum Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells Two redundant systems maintain levels of resident proteins within the yeast endoplasmic reticulum COPI-independent anterograde transport: cargo-selective ER to Golgi protein transport in yeast COPI mutants Brefeldin As effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic Brefeldin A causes a microtubule-mediated fusion of the trans-Golgi network and early endosomes The morphology but not the function of endosomes and lysosomes is altered by brefeldin A Post-Golgi membrane traffic: brefeldin A inhibits export from distal 2870 Bypassing the Golgi apparatus Golgi compartments to the cell surface but not recycling TGN38 and its orthologues: roles in post-TGN vesicle formation and maintenance of TGN morphology The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family Modulation of cellular cholesterol transport and homeostasis by Rab11 Rab8-dependent recycling promotes endosomal cholesterol removal in normal and sphingolipidosis cells A new paradigm for membrane-organizing and -shaping scaffolds Caveolins and membrane cholesterol Heterogeneous distribution of filipin-cholesterol complexes across the cisternae of the Golgi apparatus H-ras but not K-ras traffics to the plasma membrane through the exocytic pathway Palmitoylation: policing protein stability and traffic H-Ras does not need COPI-or COPII-dependent vesicular transport to reach the plasma membrane Multiple pathways in trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin-43, occurs after exit from the ER Pathways and control of connexin oligomerization Functional morphology of the secretory pathway organelles in yeast Morphodynamics of the secretory pathway Effect of Brefeldin A on biosynthesis of cellular components in Candida albicans Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. The release of the COPs by BFA results in rapid disassembly of the Golgi apparatus and the redistribution of Golgi proteins to the ER and the endosomal system [31, 32] .


id: cord-104279-choywmwd
date: 1992-10-01
extension: .txt
mime: text/plain
words: 9975
sentence: 283
flesch: 41
cache: cache/cord-104279-choywmwd.txt
txt: txt/cord-104279-choywmwd.txt
title:
  Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment

summary:
  According to the vacuolar default model for yeast membrane protein sorting, not only must ER and Golgi membrane proteins have sorting signals specifying their retention in the appropriate compartment, but plasma membrane proteins are predicted to have sorting information that prevents their localization to the vacuole. Mutations in clathrin may affect both the retention of Golgi membrane proteins and the functional integrity of the sorting pathway (Seeger and Payne, 1992b) .


id: cord-313694-p2sgaypq
author: West, Christopher M.
title: Current ideas on the significance of protein glycosylation
date: 1986
extension: .txt
mime: text/plain
words: 10925
sentence: 390
flesch: 29
cache: cache/cord-313694-p2sgaypq.txt
txt: txt/cord-313694-p2sgaypq.txt
summary:
  The role of protein glycosylation in the compartmentalization and processing of mouse mammary tumor virus glycoproteins in mouse mammary tumor virus-infected rat hepatoma cells The formation of vesicular stomatitis virus (San Juan strain) becomes temperature-sensitive when glucose residues are retained on the oligosaccharides of the glycoprotein Deletions into a NI-I2-terrninal hydrophobic domain result in secretion of Rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein Glycoantigen expression is regulated both temporally and spatially during development in the cellular slime molds Dictyostelium discoideum and D. mucoroides The identification of N-linked oligosaccharides on the human CR2/Epstein-Barr virus receptor and their function in receptor metabolism, plasma membrane expression, and ligand binding Glycosylation and secretion of surfactant-associated glycoprotein A Complete glycosylation of the insulin and insulin-like growth factor 1 receptors is not necessary for their biosynthesis and function Chemistry, metabolism, and biological functions of sialic acids Effect of size and location of the oligosaccharide chain on protease degradation of bovine pancreatic ribonuclease Swainsonine inhibits glycoprotein degradation by isolated rat liver lysosomes Slime mold lectins Bacterial glycoconjugates are natural ligands for the carbohydrate binding site of discoidin I and influence its cellular compartmentalization Carbohydrate-specific receptors of the liver Hepatic clearance of serum glycoproteins The repair of the surface structure of animal cells Carbohydrate-mediated clearance of immune complexes from the circulation Carbohydrate-mediated clearance of antibody-antigen complexes from the circulation Surface carbohydrates and surface lectins are recognition determinants in phagocytosis The carbohydrate structure of porcine uteroferrin and the role of the high mannose chains in promoting uptake by the reticuloendothelial cells of the fetal liver Intracellular movement of cell surface receptors after endocytosis: resialylation of asialotransferrin receptor in human erythroleukemia cells Hyaluronic acid bonded to cell culture surfaces stimulated chondrogenesis in stage 24 limb mesenchyme cell cultures Lectin activation in Giardia lamblia by host protease: A novel hostparasite interaction Carbohydrates as recognition determinants in phagocytosis and in lectin-mediated killing of target cells Specificity of binding of a strain of uropathogenic Escherichia coli to Galcd-4Gal-containing glycosphingolipids Evidence for a malarial parasite interaction site on the major transmembrane protein of the human erythrocyte Adherent bacterial colonization in the pathogenesis of osteomyelitis Mucoproteins in relation to virus action The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion The receptor function of galactosyltransferase during cellular interactions The Biology of Glycoproteins A multivalent lacto-N-fucopentaose III-lysyllysine conjugate decompacts preimplantation mouse embryos, while the free oligosaccharide is ineffective Receptor function of mouse sperm surface galactosyltransferase during fertilization O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity Differential cell adhesion may result from nonspecific interactions between cell surface glycoproteins Specific alteration of N-CAM-mediated cell adhesion by an endoneuraminidase A heparin-bindifig domain from N-CAM is involved in neural cell-substratum adhesion Recognition of IgG by Fc receptor and complement: effects of glycosidase digestion Cell surface carbohydrates in cell recognition and response Feizi T: Cell interactions in preimplantation embryos: evidence for involvement of saccharides of the poly-Nacetyllactosamine series Mitochondrial synthesis of glycoproteins and surface properties of mitochondrial membranes Cell membranes in sponges The phosphomannosyl recognition system for intracellular and intercellular transport of lysosomal enzymes Cell-cell recognition in yeast, characterization of the sexual agglutination factors from Saccharomyces kluyveri Lymphocyte attachement to high endothelial venules during recirculation: a possible role for carbohydrates as recognition determinants Lymphocyte adhesion to immobolized polysaccharides suggests multiple carbohydrate receptors for recirculation Spontaneous glycosylation of glycosaminoglycan substrates by adherent fibroblasts Immunocytochemical demonstration of ecto-galactosyltransferase in absorptive intestinal cells Soluble lectins: a new class of extraeellular proteins Effect of choroquine on the degradation of L-fucose and the polypeptide moiety of plasma membrane glycoproteins Different half-lives of the carbohydrate and protein moieties of a 110000 dalton glycoprotein isolated from plasma membraned of rat liver Structural determinants of the capacity of heparin to inhibit the proliferation of vascular smooth muscle cells. This observation has led to the suggestion that cell surface carbohydrate might also establish a convection and diffusionqimited region around the cell in an area thick enough to be named, in certain cell types, the glycocalyx or fuzz.


id: cord-255027-xsuialnn
author: Kellokumpu, Sakari
title: Glycosyltransferase complexes in eukaryotes: long-known, prevalent but still unrecognized
date: 2015-10-17
extension: .txt
mime: text/plain
words: 11162
sentence: 434
flesch: 40
cache: cache/cord-255027-xsuialnn.txt
txt: txt/cord-255027-xsuialnn.txt
summary:
  Namely, several different studies carried out during the late 1960s and early 1970s already showed that glycosyltransferases tend to exist as enzyme complexes in the cells. It involves several core structure forming enzyme complexes that can potentially compete with other enzymes that use the same GalNAc as an acceptor, such as sialylation of the initiating GalNAc to form the Tn-antigen.


id: cord-018572-e2qq4ngq
author: Jackson, Catherine L.
date: 2014-05-22
extension: .txt
mime: text/plain
words: 10531
sentence: 307
flesch: 35
cache: cache/cord-018572-e2qq4ngq.txt
txt: txt/cord-018572-e2qq4ngq.txt
title:
  Arf Proteins and Their Regulators: At the Interface Between Membrane Lipids and the Protein Trafficking Machinery

summary:
  The spatiotemporal control of Arf protein function is mediated by regulators of Arf GTP binding and GTP hydrolysis (Fig. 8.1d ). These phospholipids can influence the sorting of membrane proteins within the PM, and are important for the formation of clathrin-coated pits during endocytosis.


id: cord-287477-aios0h8s
author: Sicari, Daria
title: Role of the early secretory pathway in SARS-CoV-2 infection
date: 2020-07-28
extension: .txt
mime: text/plain
words: 6498
sentence: 237
flesch: 35
cache: cache/cord-287477-aios0h8s.txt
txt: txt/cord-287477-aios0h8s.txt
summary:
  Several protein disulfide isomerase inhibitors are available, including PACMA31, which specifically targets the cysteine residue present in the active site of protein disulfide isomerase proteins and blocking their capability to bind cargoes (Kaplan et al., 2015) . Among the 66 U.S. Food and Drug Administration-approved drugs, they suggested PS30613 (ER protein processing, Sec61 inhibitor); IHVR-19029 (ER protein processing); FK-506 (FKBP binder); zotatifin (EIF4E2/H, eiF4a inhibitor); rapamycin and rapalogs (LARP1, FKBP15, FKBP7/10, mammalian target of rapamycin inhibitor); diverse SIGMAR1/2 modulators, such as chloroquine, PB28, and haloperidol E-52862, PD-144418, and RS-PPCC; as well as diverse SIGMAR1/2 direct modulators, such as the antagonist PD-144418 and the agonist RS-PPCC (Gordon et al., 2020) .


id: cord-009371-ub4p4ngr
author: Mollenhauer, Hilton H.
date: 1990-05-07
extension: .txt
mime: text/plain
words: 12405
sentence: 516
flesch: 45
cache: cache/cord-009371-ub4p4ngr.txt
txt: txt/cord-009371-ub4p4ngr.txt
title:
  Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity

summary:
  [46] reported a shift to higher density of Golgi apparatus membranes from monensin-treated barley cells, a response no obvious from work with mammalian cells. These data imply that the monensin-induced swelling of Golgi apparatus cisternae involves a proton gradmnt at or near the mature poles of the Golgi apparatus Because monensin induces a 1 1 Na+/H + exchange, and since the Van't Hoft factors for H + and Na + are practically the same [159] , the osmolanty of the cell content should not increase to cause swelhng without a net proton influx One explanation would be that the pH of Golgi apparatus vesicles is highly regulated Proton translocatlng ATP hydrolyzing enzymes (H +-ATPases) are associated with several components of cells that develop acidic intermrs such as endosomes, coated vesicles, lysosomes, and trans Golgl apparatus Clsternae


id: cord-017866-h5ttoo0z
author: Bowman, Grant R.
title: Biogenesis of Dense-Core Secretory Granules
date: 2010-05-27
extension: .txt
mime: text/plain
words: 13387
sentence: 461
flesch: 33
cache: cache/cord-017866-h5ttoo0z.txt
txt: txt/cord-017866-h5ttoo0z.txt
summary:
  In cells producing mucous-containing DCGs, the cis and medial Golgi appear as flat cisternae, and secretory proteins are evenly distributed in their lumina. Th eir roles in secretion from neuroendocrine cells and as markers for neuroendocrine neoplasia Chro mogranin B (secretogranin I) promotes sorting to the regulated secretory pathway of processing intermediates derived from a peptide hormone precursor Gorr SUo Aggregation chaperones enhance aggregation and storage of secretory proteins in endocrine cells In vitro aggregation of the regulated secretory protein chromogranin A Reconstitution in vitro of the pH-dependent aggregation of pancreatic zymogens en route to the secretory granule: Implication of GP-2 Exocrine granule specific packaging signals are present in the polypeptide moiery of the pancreatic granule membrane protein GP2 and in amylase: Implications for protein targeting to secretory granules Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic beta-cells Identification of a novel sorting determinant for the regulated pathway in the secretory protein chromogranin A Sorting of three secretory proteins to distinct secretory granules in acidophilic cells of cow anterior pituitary Multiple neuropept ides derived from a common precursor are differentially packaged and transported Structure et ultrastructure de Lacrymaria olor (O.F.M. 1786) Cocrystallization of proinsulin and insulin Vayssie Let aI. A large multigenic family codes for the polypeptides of the crystalline trichocyst matrix in Paramecium Protein secretion in Tetrahymena thermophila.


id: cord-026010-61j07gq3
author: Elbein, Alan D.
title: Alkaloid Glycosidase Inhibitors
date: 2010-06-03
extension: .txt
mime: text/plain
words: 14892
sentence: 266
flesch: 11
cache: cache/cord-026010-61j07gq3.txt
txt: txt/cord-026010-61j07gq3.txt
summary:
  mM#[ 120 Furthermore\ the compound also inhibited the aryl!a!mannosidase IC 49 of 399 nM# and the aryl!b!mannosidase IC 49 of 049 mM#\ although it clearly preferred a!linkages[ In cell culture studies\ mannonolactam amidrazone gave rise to glycoproteins with the same type of high mannose oligosaccharide as seen with DMJ and kifunensine[ Thus inhibition of Golgi mannosidase I and:or ER mannosidase# appears to prevent trimming of most if not all mannose residues[ 120 The designers of this compound 129 hypothesize that the reason that it is so e}ective as a general mannosidase inhibitor is that it is the _rst analogue of mannose that mimics the true half!chair conformation of the cationic intermediate that is believed to be involved in catalysis of the a!mannosides[ Mannonolactam should serve as a model for the synthesis of more speci_c mannosidase inhibitors[ As mentioned earlier\ I and glucosidase II# are discussed below[ Australine 7# is a tetrahydroxypyrrolizidine alkaloid that was found in the same seeds that contain castanospermine\ namely Castanospermum australe[ 10 However\ australine is present in the seeds in much lower amounts than is castanospermine[ This compound is a good inhibitor of fungal amyloglucosidase\


id: cord-326015-ky4y2xjt
author: Füllekrug, Joachim
title: Protein sorting in the Golgi complex
date: 1998-08-14
extension: .txt
mime: text/plain
words: 3945
sentence: 158
flesch: 44
cache: cache/cord-326015-ky4y2xjt.txt
txt: txt/cord-326015-ky4y2xjt.txt
summary:
  ERGIC-53 carries a cytosolic endoplasmic reticulum determinant interacting with COP II A hypothesis on the tra¤c of MG160, a medial Golgi sialoglycoprotein, from the trans-Golgi network to the Golgi cisternae Warren, Evidence for recycling of the resident medial/trans Golgi enzyme, N-acetylglucosaminyltransferase I, in ldlD cells Localization of a yeast early Golgi mannosyltransferase, Och 1p, involves retrograde transport Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER Retention of membrane proteins by the endoplasmic reticulum Do other Golgi resident proteins display similar signals?


id: cord-016971-7esuj4ye
author: Mironov, Alexander A.
title: The Golgi apparatus and main discoveries in the field of intracellular transport
date: 2008
extension: .txt
mime: text/plain
words: 2452
sentence: 64
flesch: 29
cache: cache/cord-016971-7esuj4ye.txt
txt: txt/cord-016971-7esuj4ye.txt
summary:
  Two types derived from opposite faces of the Golgi complex in developing granulocytes Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine The organization of endoplasmic reticulum export complexes Membrane dynamics at the endoplasmic reticulum-Golgi interface COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum Anterograde transport of algal scales through the Golgi complex is not mediated by vesicles The Golgi apparatus: from discovery to contemporary studies Procollagen traverses the Golgi stack without leaving the lumen of cisternae: evidence for cisternal maturation Diffusional mobility of Golgi proteins in membranes of living cells The Golgi apparatus and main discoveries * 11 Coatomer interaction with di-lysine endoplasmic reticulum retention motif Observations of the Golgi substance with the electron microscope Receptor and protein kinase C-mediated regulation of ARF binding to the Golgi complex AP-3: an adaptor-like protein complex with ubiquitous expression AP-4, a novel protein complex related to clathrin adaptors Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum Camillo Golgi and the discovery of the Golgi apparatus Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q-and R-SNAREs Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells Synthesis and turnover of membrane proteins in rat liver: an examination of the membrane flow hypothesis Functions of coated vesicles during protein absorption in the rat vas deferens A cisternal maturation mechanism can explain the asymmetry of the Golgi stack On the site of sulfation in the chondrocyte Golgi C (1898a) The trans ER (Novikoff 1964; Novikoff et al. 1964) 1964 GERL concept (Novikoff 1964) 1964 Isolation of Golgi membranes from cells (Morr e and Mollenhauer 1964) 1964


id: cord-020788-a33vcapl
author: Gottardi, Cara J.
title: Signals and Mechanisms of Sorting in Epithelial Polarity
date: 2008-05-22
extension: .txt
mime: text/plain
words: 13913
sentence: 416
flesch: 29
cache: cache/cord-020788-a33vcapl.txt
txt: txt/cord-020788-a33vcapl.txt
summary:
  Yeast syntaxins Ssolp and Sso2p belong to a family of related membrane proteins that function in vesicular transport Role ofmicrotubules in polarized delivery of apical membrane proteins to the brush border ofthe intestinal epithelium Distribution of transport proteins over animal cell membranes Podocytosis of small molecules and ions by caveolae The calmodulin antagonist, W-13, alters transcytosis, recyclingand morphology ofthe endocyticpathway in Madine-Darby canine kidney cells Mutational and secondary structural analysis of the basolateral sorting signal of the polymeric immunoglobulin receptor Vesicular stomatitis virus glycoprotein is sorted and concentrated during export from the endoplasmic reticulum Cloning of a rab3 isotype predominately expressed in adipocytes The NPXY internalization signal of the LDL receptor adopts a reverse turn conformation Biogenesis of the rat hepatocyte plasma membrane in vivo: Comparison of the pathways taken by apical and basolateral proteins using subcellular fractionation. The differential distribution of membrane proteins between the plasmalemmal surfaces of polarized epithelial cells enables these cells to both respond to and effect changes upon their environment in a directed fashion.


id: cord-306067-ldn17pj8
author: Inoue, Satoshi
date: 2003-12-19
extension: .txt
mime: text/plain
words: 6380
sentence: 201
flesch: 54
cache: cache/cord-306067-ldn17pj8.txt
txt: txt/cord-306067-ldn17pj8.txt
title:
  Assembly of the silk fibroin elementary unit in endoplasmic reticulum and a role of L‐chain for protection of α1,2‐mannose residues in N‐linked oligosaccharide chains of fibrohexamerin/P25

summary:
  [1] and exists either in a 30-kDa (major) or 27-kDa (minor) molecular form [1, 5] , which has been suggested to have different compositions of oligosaccharide chains However, considering the facts that oligosaccharide chains play important roles in the assembly of vesicular stomatitis viral glycoprotein [28, 29] , influenza virus hemagglutinin [30, 31] , and IgM [32] in ER, a further role of N-linked oligosacharide chains of fhx/P25 in the process of assembly of the elementary unit in ER may be speculated.


id: cord-332484-qy8vj6uu
author: Pierini, Roberto
date: 2009-04-01
extension: .txt
mime: text/plain
words: 4260
sentence: 177
flesch: 36
cache: cache/cord-332484-qy8vj6uu.txt
txt: txt/cord-332484-qy8vj6uu.txt
title:
  Modulation of membrane traffic between endoplasmic reticulum, ERGIC and Golgi to generate compartments for the replication of bacteria and viruses

summary:
  Targeting of host Rab GTPase function by the intravacuolar pathogen Legionella pneumophila Legionella pneumophila proteins that regulate Rab1 membrane cycling A bifunctional bacterial protein links GDI displacement to Rab1 activation The Legionella pneumophila effector protein DrrA is a Rab1 guanine nucleotideexchange factor Legionella subvert the functions of Rab1 and Sec22b to create a replicative organelle RNA interference analysis of Legionella in Drosophila cells: exploitation of early secretory apparatus dynamics Legionella pneumophila replication vacuole formation involves rapid recruitment of proteins of the early secretory system Parallels among positive strand RNA viruses, reverse transcribing viruses and double strand RNA viruses Inhibition of poliovirus RNA synthesis by brefeldin A Involvement of cellular membrane traffic proteins in poliovirus replication COPI activity coupled with fatty acid biosynthesis is required for viral replication Identification of essential host factors affecting tombusvirus RNA replication based on the yeast Tet promoters Hughes collection Poliovirus proteins induce membrane association of GTPase ADP ribosylation factor Hijacking components of the cellular secretory pathway for replication of poliovirus RNA A critical role of a cellular membrane traffic protein in poliovirus RNA replication Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A A viral protein that blocks Arf-1 mediated COP-I assembly by inhibiting the guanine nucleotide exchange factor GBF1 Effects of picornavirus 3A proteins on protein transport and GBFIdependent COP-I recruitment Molecular determinants of the interaction between coxsackievirus protein 3A and guanine nucleotide exchange factor GBF1 Poliovirus protein 3A binds and inactivates LIS1, causing block of membrane protein trafficking and deregulation of cell division Poliovirus infection blocks ERGIC-to-Golgi trafficking and induces microtubule-dependent disruption of the Golgi complex Inhibition of cellular protein secretion by picornaviral 3A proteins Effects of foot-and-mouth disease virus nonstructural proteins on the structure and function of the early secretory pathway: 2BC but not 3A blocks endoplasmic reticulum-to-Golgi transport Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae Cellular COPII proteins are involved in production of the vesicles that form the poliovirus replication complex Intracellular location and translocation of silent and active poliovirus replication complexes Biogenesis of cytoplasmic membranous vesicles for plant potyvirus replication occurs at endoplasmic reticulum exit sites in a COPI-and COPII-dependent manner Subversion of cellular autophagosomal machinery by RNA viruses Inhibition of the secretory pathways by foot-and-mouth disease virus 2BC protein is reproduced by coexpression of 2B with 2C, and the site of inhibition is determined by the subcellular localisation of 2C Inhibition of protein trafficking by coxsackievirus b3: multiple viral proteins target a single organelle Reticulon 3 binds the 2C protein of enterovirus 71 and is required for viral replication Hepatitis C virus RNA polymerase and NS5A complex with a SNARE-like protein Human VAP-B is involved in hepatitis C virus replication through interaction with NS5A and NS5B Norwalk virus nonstructural protein p48 forms a complex with the SNARE regulator VAP-A and prevents cell surface expression of vesicular stomatitis virus G protein TBC1D20 is a Rab1 GTPase-activating protein that mediates hepatitis C virus replication A Rab-GAP TBC domain protein binds Hepatitis C virus NS5A and mediates virus replication Participation of Rab5, an early endosome protein, in hepatitis C virus RNA replication machinery Brucella intracellular replication requires trafficking through the late endosomal/lysosomal compartment Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activation Localization and membrane topology of coronavirus nonstructural protein 4: involvement of the early secretory pathway in replication The unique architecture of Bunyamwera virus factories around the Golgi complex Fowlpox virus encodes nonessential homologs of cellular alpha-SNAP, PC-1, and an orphan human homolog of a secreted nematode protein African Swine Fever virus causes microtubule-dependent dispersal of the trans-Golgi network and slows delivery of membrane protein to the plasma membrane The subcellular distribution of multigene family 110 proteins of African Swine Fever virus is determined by differences in C-terminal KDEL endoplasmic reticulum retention motifs


id: cord-022235-6ircruag
author: Pugsley, Anthony P.
title: Later stages in the eukaryotic secretory pathway
date: 2012-12-02
extension: .txt
mime: text/plain
words: 15247
sentence: 604
flesch: 50
cache: cache/cord-022235-6ircruag.txt
txt: txt/cord-022235-6ircruag.txt
summary:
  As we shall see, the physical separation of Golgi enzymes is also indicated by the succession of posttranslational modifications to which secretory proteins are subjected, by in situ immunocytochemistry, and by the separation of Golgi-derived vesicles containing different en zymes. How then do secretory proteins move between these compart ments, and is the secretory pathway a continuous gradient of secretory organelles or are they functionally and structurally independent?


id: cord-298503-l60cdllh
author: Saraste, J.
date: 2015-08-20
extension: .txt
mime: text/plain
words: 9555
sentence: 206
flesch: 23
cache: cache/cord-298503-l60cdllh.txt
txt: txt/cord-298503-l60cdllh.txt
title:
  Intermediate Compartment: A Sorting Station between the Endoplasmic Reticulum and the Golgi Apparatus

summary:
  (Presley et al., 2002; Marie et al., 2009 ). It releases COPI coats, disassembles the Golgi stacks and redistributes Golgi components to the ER, whereas cycling proteins (such as p58/ERGIC-53/LMAN1 and the KDELreceptor) are arrested in the drug-resistant IC elements (Lippincott-Schwartz et al., 1990; Saraste and Svensson, 1991; Tang et al., 1995b; Füllekrug et al., 1997; Ward et al., 2001; Marie et al., 2009) .


id: cord-354726-b9xvycyk
title: Envelope glycoprotein interactions in coronavirus assembly
date: 1995-10-02
extension: .txt
mime: text/plain
words: 8029
sentence: 306
flesch: 47
cache: cache/cord-354726-b9xvycyk.txt
txt: txt/cord-354726-b9xvycyk.txt
summary:
  3 B. Virions containing labeled S protein appeared in the medium after a 30-min lag period. In contrast, labeled M protein started to appear in the culture medium already during the 15-30 min chase period, increased rapidly during the 30--60 min chase period, and declined thereafter.


id: cord-351964-hduv0ur4
date: 1987-09-01
extension: .txt
mime: text/plain
words: 7643
sentence: 267
flesch: 47
cache: cache/cord-351964-hduv0ur4.txt
txt: txt/cord-351964-hduv0ur4.txt
title:
  Sorting of progeny coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells

summary:
  Visualization of acidic organelles in intact cells by electron microscopy Vesicles and cisternae in the trans Golgi apparatus of human fibroblasts are acidic compartments Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts Phase separation of integral membrane proteins in Triton X-114 solution The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer Biosynthesis of N-and O-linked oligosaccharides of the low density lipoprotein receptor Assembly of the Coronvidrae An enzymatic assay reveals that proteins destined for the apical or basolateral domains of an epithelial cell share the same late Golgi compartments Vesicular Stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line Efficient transport of Semliki Forest virus glycoproteins through a Golgi complex morphologically altered by Uukuniemi virus glycoproteins Passage of viral membrane proteins through the Golgi complex The trans Golgi network; sorting at the exit site of the Golgi complex Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells Pathways of protein secretion in eukaryotes Localization of two intracellular forms of the vesicular stomatitis viral glycoprotein Sorting of a plasma membrane protein occurs before it reaches the cell surface in cultured epithelial cells Biogenesis of epithelial cell polarity: intracellular sorting and vectorial exocytosis of an apical plasma membrane glycoprotein Chloroquine diverts ACTH from a regulated to a constitutive pathway in ART20 cells Secretory protein targeting in a pituitary cell line: differential transport of foreign secretory proteins to distinct secretory pathways The carbohydrates of the O-glycosidically linked oligosaccharides of glycoprotein El Coronavirus glycoprotein El, a new type of viral glycoprotein Intracellular sorting and basolateral appearance of the G protein of vesicular stomatitis virus in MDCK cells Use of immunocytocbemical techniques in studying the biogenesis of cell surfaces in polarised epithelia Viral glycoprotein destined for apical or basolateral plasma membrane domains traverse the same Golgi apparatus during their intracellufar transport in doubly infected MDCK cells Identification of two epitopes in the carboxy terminal 15 amino acids of the El glycoprotein of Murine Hepatitis virus A59 by using hybrid proteins Infection of ART20 murine pituitary tumour cells by mouse hepatitis virus strain A59: virus budding is restricted to the Golgi region Clathrin-coated vesicular transport of secretory proteins during the formation of ACTH-containing secretory granules in ART20 cells Replication of coronavirus MHV-A59 in sac-cells: determination of the first site of budding of progeny virus We thank our colleagues Stella Hurtiey, Jean Gruenberg, and Gareth Griffiths (European Molecular Biological Laboratory, Heidelberg) for their constructive comments on the manuscript, and Ines Benner who patiently and skillfully typed this manuscript. In other cells virions and condensing secretory proteins were in different dilations of the complex trans-Golgi network (Fig. 1) .


id: cord-303153-z7bdiuvx
author: Ulasli, Mustafa
date: 2010-01-20
extension: .txt
mime: text/plain
words: 8723
sentence: 374
flesch: 52
cache: cache/cord-303153-z7bdiuvx.txt
txt: txt/cord-303153-z7bdiuvx.txt
title:
  Qualitative and quantitative ultrastructural analysis of the membrane rearrangements induced by coronavirus

summary:
  These are the characteristic DMVs induced by CoV (Svoboda et al., 1962; David-Ferreira and Manaker, 1965; Ruebner et al., 1967; Pedersen et al., 1999; Gosert et al., 2002; Snijder et al., 2006; Knoops et al., 2008) . -Caplen et al., 1984; David-Ferreira and Manaker, 1965; Ruebner et al., 1967; Salanueva et al., 1999; Escorcia et al., 2002; Gosert et al., 2002; Snijder et al., 2006; Stertz et al., 2007; Knoops et al., 2008;


id: cord-268527-wbfnhedy
author: Smith, Sylvia B.
title: Transient hyperglycosylation of rhodopsin with galactose
date: 1991-10-31
extension: .txt
mime: text/plain
words: 8572
sentence: 385
flesch: 54
cache: cache/cord-268527-wbfnhedy.txt
txt: txt/cord-268527-wbfnhedy.txt
summary:
  ConA purified rhodopsin labeled with [%]methionine and [Hlglucosamine was incubated at 3 7°C for 42 hr with 20 IT of N-glycanase (Genzyme, Boston, MA) in 0.15% SDS, 10 mM /$mercaptoethanol, 100 ,LLM PMSF. The percentage of ConA purified rhodopsin which contained galactose was determined from the radioactivity of the rhodopsin band of the gels of the RCA eluate as c.ompared to the ConA gels for each treatment group.


id: cord-298251-u36lb44w
author: Donaldson, Julie G.
title: Arf Family G Proteins and their regulators: roles in membrane transport, development and disease
date: 2011-05-18
extension: .txt
mime: text/plain
words: 10742
sentence: 360
flesch: 35
cache: cache/cord-298251-u36lb44w.txt
txt: txt/cord-298251-u36lb44w.txt
summary:
  c | In primary cilia, ARL6 recruits the BBSome coat complex that facilitates the transport of membrane proteins into the cilium. Recycling endosomes return membrane proteins that are important for cell adhesion and migration back to the plasma membrane 34, 35 .


id: cord-329515-ra20actc
date: 1994-07-01
extension: .txt
mime: text/plain
words: 9112
sentence: 338
flesch: 47
cache: cache/cord-329515-ra20actc.txt
txt: txt/cord-329515-ra20actc.txt
title:
  Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers

summary:
  oligomers, suggesting that self-association of p63 protein may serve as a major mechanism for retention. A comparison of the behavior of wildtype and mutant p63 proteins in this assay revealed a perfect correlation between the formation of large oligomers and correct intracellular retention.


id: cord-104268-q1jx0n0l
date: 1995-11-02
extension: .txt
mime: text/plain
words: 11089
sentence: 379
flesch: 42
cache: cache/cord-104268-q1jx0n0l.txt
txt: txt/cord-104268-q1jx0n0l.txt
title:
  Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha 1,2-mannosyltransferase to a medial-Golgi compartment

summary:
  Implications for the organization of the ER-to-Golgi pathway Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers Selective and immediate effects of clathrin heavy chain mutations on Golgi membrane protein retention in Saccharomyces cerevisiae Enzymes associated with glycosylation A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae Isolation of a matrix that binds medial-Golgi enzymes A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal The signal for Golgi retention of bovine 131,4-galactosyltransferase is the transmembrane domain Oligomerization of a membrane protein correlates with its retention in the Golgi complex Mutation of a tyrosine localization signal in the cytosolic tail of Kex2p protease disrupts Golgi retention and results in default transport to the vacuole Dynamic retention of TGN membrane proteins in Saccharomyces cerevisiae The 17-residue transmembrane domain of 13-galactoside ct2,6-sialyltransferase is sufficient for Golgi retention The SXYQRL sequence in the cytoplasmic domain of TGN38 plays a major role in the trans-Golgi network localization The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases Golgi retention mechanism of 13-1,4-galactosyltransferase Cloning and analysis of the Saccharomyces cerevisine MNN9 and MNN1 genes required for complex glycosylation of secreted proteins We thank Dr. Annette Herscovics for critically reading the manuscript, the members of the Bussey laboratory for helpful comments and suggestions, Antony Cooper and Kathryn Hill for all their help and advice, Tom Stevens for kindly providing strains, plasmids, and antibodies, Anne Camirand for advice with the mannosyltransferase assays, Rowan Chapman for discussion and plasmids, V. Mackay and C. Yip for the MNN1 clone, Carole Smith and Guy I'Heureux for photographic work, and Diane Oki for assistance with manuscript preparation. The results demonstrate that the NH2-terminal cytoplasmic domain is necessary for correct Kre2p Golgi localization whereas, the membrane-spanning and stem domains are dispensable.


id: cord-104241-cqvnxsbo
author: Bryant, Nia J.
date: 1997-01-27
extension: .txt
mime: text/plain
words: 8766
sentence: 250
flesch: 43
cache: cache/cord-104241-cqvnxsbo.txt
txt: txt/cord-104241-cqvnxsbo.txt
title:
  Two Separate Signals Act Independently to Localize a Yeast Late Golgi Membrane Protein through a Combination of Retrieval and Retention

summary:
  trast, TGN membrane proteins (A-ALP and Kex2p) fractionated near the bottom of the gradient. vps27 mutant cells accumulate an exaggerated form of the PVC (the class E compartment) containing endocytosed proteins, as well as TGN membrane proteins and proteins en route to the vacuole, such as activated vacuolar proteases ( Fig. 3 ; Raymond et al., 1992; Piper et al., 1995) .


id: cord-265887-g5zhoyo9
author: Mukherjee, Shruti
date: 2020-08-11
extension: .txt
mime: text/plain
words: 9093
sentence: 327
flesch: 30
cache: cache/cord-265887-g5zhoyo9.txt
txt: txt/cord-265887-g5zhoyo9.txt
title:
  Host-membrane interacting interface of the SARS coronavirus envelope protein: Immense functional potential of C-terminal domain

summary:
  Analysis of constructed E gene mutants of mouse hepatitis virus confirms a pivotal role for E protein in coronavirus assembly Coronavirus pseudoparticles formed with recombinant M and E proteins induce alpha interferon synthesis by leukocytes Membrane Lipid Composition: Effect on Membrane and Organelle Structure, Function and Compartmentalization and Therapeutic Avenues Protein aggregation in a membrane environment Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains Biological phase separation: cell biology meets biophysics Membrane lipids: where they are and how they behave SARS coronavirus E protein in phospholipid bilayers: an x-ray study Membrane topology and insertion of membrane proteins: search for topogenic signals Another triple-spanning envelope protein among intracellularly budding RNA viruses: the torovirus E protein Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding Identifying SARS-CoV-2 Coevolution in defining the functional specificity Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes Improving the accuracy of transmembrane protein topology prediction using evolutionary information Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web server Analysis of membrane and surface protein sequences with the hydrophobic moment plot Trends in substitution models of molecular evolution The rapid generation of mutation data matrices from protein sequences MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms Membrane topology of coronavirus E protein Characterization of the coronavirus mouse hepatitis virus strain A59 small membrane protein E Two deletion variants of Middle East respiratory syndrome coronavirus found in a patient with characteristic symptoms Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus Coronavirus virulence genes with main focus on SARS-CoV envelope gene Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication Hexamethylene amiloride blocks E protein ion channels and inhibits coronavirus replication 2020. (44) The absence of a canonical cleaved signal sequence suggests equal likelihood for the protein to be a type II (with its C-terminal targeted to the ER lumen) or type III (with its N-terminal targeted to the ER lumen) membrane protein.


id: cord-299281-5z1xminb
title: Oligomerization of a membrane protein correlates with its retention in the Golgi complex
date: 1993-09-02
extension: .txt
mime: text/plain
words: 8182
sentence: 322
flesch: 47
cache: cache/cord-299281-5z1xminb.txt
txt: txt/cord-299281-5z1xminb.txt
summary:
  We are currently constructing a series of Gml proteins with truncated cytoplasmic tails to determine if these proteins are correctly targeted and form oligomers. When the membrane-spanning domain of a protein which is efficiently delivered to the plasma membrane (VSV G protein) is replaced with m1, the resulting chimera (Gm1) is retained in the Golgi (Swift, A. M., and C. E. Machamer. 1991.


id: cord-255981-3zvwu5bd
author: Bui, Quynh Trang
date: 2009-08-11
extension: .txt
mime: text/plain
words: 7146
sentence: 225
flesch: 39
cache: cache/cord-255981-3zvwu5bd.txt
txt: txt/cord-255981-3zvwu5bd.txt
title:
  Large Arf1 guanine nucleotide exchange factors: evolution, domain structure, and roles in membrane trafficking and human disease

summary:
  The secretory pathway transports secreted and membrane proteins synthesized in the ER to their Wnal destination (plasma membrane (PM), cell exterior or intracellular organelle). The C. parvum GBF protein shares a high level of homology with plant GBF proteins such as the Arabidopsis thaliana GNOM protein.


id: cord-104231-fi8pskod
date: 1994-04-02
extension: .txt
mime: text/plain
words: 8280
sentence: 348
flesch: 54
cache: cache/cord-104231-fi8pskod.txt
txt: txt/cord-104231-fi8pskod.txt
title:
  The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network

summary:
  Cells expressing CIM or CD4 hybrid proteins were labeled with [35S]methionine/cysteine for 20 min (see Materials and Methods) and either lysed immediately or chased with excess cold methionine and cysteine for 2 h. Immunoprecipitates were treated with neuraminidase or mocktreated and analyzed by SDS-PAGE (Fig. 8) . CD8 hybrid proteins were constructed such that the first 153 amino acids of the extracellular domain of c~CD8 were preserved.


id: cord-264996-og3sg0qw
author: Howell, Gareth J.
title: Cell Biology of Membrane Trafficking in Human Disease
date: 2006-09-17
extension: .txt
mime: text/plain
words: 20376
sentence: 623
flesch: 29
cache: cache/cord-264996-og3sg0qw.txt
txt: txt/cord-264996-og3sg0qw.txt
summary:
  Sorting of proteins from donor to target membranes involves the recognition of cytoplasmic sequences in membrane proteins by clathrin-associated AP complexes. However, their exact role in the internalization of membrane proteins and soluble protein ligands is controversial.


id: cord-022313-2675sjlf
author: Elbein, Alan D.
title: The Use of Glycosylation Inhibitors to Study Glycoconjugate Function
date: 2012-12-02
extension: .txt
mime: text/plain
words: 21564
sentence: 664
flesch: 34
cache: cache/cord-022313-2675sjlf.txt
txt: txt/cord-022313-2675sjlf.txt
summary:
  The most common types of glycoproteins that are present in eucaryotic cells, as cell surface proteins, membrane proteins, and secretory proteins, are those having oligosaccharides attached to the amide nitro gen of asparagine (N-linked or asparagine-linked) (10) . As a result of increasing interest in this area of research, a number of new α-mannosidase activi ties have been described and current work is aimed at trying to under stand how these various enzymes function in the removal of α-linked mannoses from eucaryotic cell glycoproteins.


id: cord-287815-alv30uk5
author: Mellman, Ira
title: The Golgi complex: In vitro veritas?
date: 1992-03-06
extension: .txt
mime: text/plain
words: 9337
sentence: 301
flesch: 37
cache: cache/cord-287815-alv30uk5.txt
txt: txt/cord-287815-alv30uk5.txt
summary:
  Nevertheless, the observed conservation of Golgi proteins between S. cerevisiae and mammals is most encouraging for our ability to confirm in living cells the function of components identified in vitro. I. Stereological studies Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane The dynamic nature of the Golgi complex Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast Membrane traffic in endocytosis: insights from cell-free assays Reconstitution of steps in the constitutive secretorypathwayin permeabilized cells Three-dimensional architecture of the cortical region of the Golgi-apparatus in rat spermatids Reclustering of scattered Golgi elementsoccurs along microtubules Movement of internalized ligand-receptor complexes along a continuous endosomal reticulum Derived protein sequence, oligosaccharides, and membrane insertion of the 120 kD lysosomal membrane protein (Igpl20): identification of a highly conserved family of lysosomal membrane glycoproteins A recycling pathway between the endoplasmic reticulum and the Golgi apparatus for retention of unassembled MHC class I molecules Basolateral sorting in MDCKcells requiresadistinct cytoplasmicdomain determinant Selective inhibition of transcytosis by brefeldin A in MDCK cells Protein sulfation on tyrosine Biosynthetic protein transport in the secretory pathway Localization of mitochondria in living cells with rhodamine 123 Fluoride is not an activator of the smaller (20-25 kDa) GTP-binding proteins The rate of bulk flow from the Golgi to the plasma membrane Pathways of protein secretion in eukaryotes Elrefeldin A: cytosolic coat protein assembly, organelle structure, and membrane traffic Assembly of asparagine-linked a~ligosaccharides The biogenesis of lysosomes Dynamic behavior of endoplasmic reticulum in living cells The neuronal endomembranesystem Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the Golgi Hepatoma secretory proteins migrate from the rough endoplasmic reticulum to Golgi at characteristic rates Evidence for a molecular distinction between Golgi and cell surface forms of Immunocytochemical analysis of the transfer from the intermediate compartment to the Golgi complex of VSV G protein The El glycoprotein of an avian coronavirus is targeted to the cis Golgi complex Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack Three dimensional structure of endosomes in BHK-21 cells Scale formation rn algae Novel blockade by brefeldin A of intracellular transport of secretory transport of secretory proteins in cultured rat hepatocytes Sequences within and adjacent to the transmembrane segment of a-2,6sialyltransferase specify Golgi retention Recycling glycoproteins do not return to the cis-Golgi The membrane spanning domain of 8-l ,4-galactosyltransferase specifies rrans Golgi localization A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae Molecular trapping of a fluorescent ceramide analogue at the Golgi apparatus of fixed cells: interaction with endogenous lipids provides a trans-Golgi marker for both light and electron microscopy A novel fluorescent ceramide analog for studying membrane traffic in animal cells: accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor Intracellular aspects of the process of protein secretion Clathnn, adaptors, and sorting Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment Control of protein exit from the endoplasmic reticulum Recycling of proterns between the endoplasmic reticulum and Golgi complex Brosyntheticprotetn transport and sorting by the endoplasmic reticulum and Golgi Three-dimensional electron microscopy: StructureoftheGolgr apparatus Perturbation of the morphology of the trans-Golgi network following brefeldin A treatment: redistribution of aTGN-specific integral membrane protein Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles Post-translational protein modification in the endoplasmic reticulum.


id: cord-102964-zh737cjk
author: Ferraro, Francesco
title: Modulation of endothelial organelle size as an antithrombotic strategy
date: 2020-05-17
extension: .txt
mime: text/plain
words: 5560
sentence: 259
flesch: 38
cache: cache/cord-102964-zh737cjk.txt
txt: txt/cord-102964-zh737cjk.txt
summary:
  Endothelial VWF is stored in cell-specific secretory granules, Weibel-Palade bodies (WPBs), uniquely rod-like exocytic organelles generated in a wide range of lengths (0.5 to 5.0 µm). Blue asterisks: platelet aggregation in malignant melanoma of mice and humans Sepsis and septic shock: endothelial molecular pathogenesis associated with vascular microthrombotic disease Multidrug resistance protein 1 (MRP1, ABCC1), a multitasking ATPbinding cassette (ABC) transporter KLF2 provokes a gene expression pattern that establishes functional quiescent differentiation of the endothelium DNA damage triggers Golgi dispersal via DNA-PK and GOLPH3 A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells Weibel-Palade body size modulates the adhesive activity of its von Willebrand Factor cargo in cultured endothelial cells Prolonged shear stress and KLF2 suppress constitutive proinflammatory transcription through inhibition of ATF2 Image-based drug screen identifies HDAC inhibitors as novel Golgi disruptors synergizing with JQ1 Statins and the vascular endothelial inflammatory response Temperature-dependence of Weibel-Palade body exocytosis and cell surface dispersal of von Willebrand factor and its propolypeptide Contribution of platelet vs. endothelial VWF to platelet adhesion and hemostasis Cardiac glycoside toxicity: more than 200 years and counting Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation Tumor necrosis factor alphamediated reduction of KLF2 is due to inhibition of MEF2 by NF-kappaB and histone deacetylases Statin treatment before percutaneous cononary intervention Kruppel-like factor 2 (KLF2) regulates endothelial thrombotic function A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport Organelle size control systems: from cell geometry to organelledirected medicine Weibel-Palade bodies at a glance The physiological function of von Willebrand's factor depends on its tubular storage in endothelial Weibel-Palade bodies Cardiac glycosides as novel cancer therapeutic agents Cellular stress induces erythrocyte assembly on intravascular von Willebrand factor strings and promotes microangiopathy Ultralarge von Willebrand factor fibers mediate luminal Staphylococcus aureus adhesion to an intact endothelial cell layer under shear stress Statins exert endothelial atheroprotective effects via the KLF2 transcription factor The role of von Willebrand factor in thrombus formation Interaction of von Willebrand factor with platelets and the vessel wall In vivo imaging analysis of the interaction between unusually large von Willebrand factor multimers and platelets on the surface of vascular wall Biochemistry and genetics of von Willebrand factor Kruppel-like factor 2 as a novel mediator of statin effects in endothelial cells G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis Microtubules and the organization of the Golgi complex Identification and characterization of Cardiac Glycosides as senolytic compounds Pathophysiology of thrombotic thrombocytopenic purpura The shear stress-induced transcription factor KLF2 affects dynamics and angiopoietin-2 content of Weibel-Palade bodies Cell adhesion mechanisms in platelets Thrombosis in diabetes: a shear flow effect? Mechanoenzymatic cleavage of the ultralarge vascular protein von Willebrand factor Affects (no defined mechanism) Integrin beta-2 (ITGB2) and inhibits Integrin alpha-L (ITGAL) High affinity nerve growth factor receptor (NTRK1), Macrophage colony-stimulating factor 1 receptor (CSF1R), Platelet-derived growth factor receptor alpha (PDGFRA), Platelet-derived growth factor receptor beta (PDGFRB)


id: cord-322516-wekvet6f
author: Maceyka, Michael
date: 1997-12-15
extension: .txt
mime: text/plain
words: 6196
sentence: 252
flesch: 48
cache: cache/cord-322516-wekvet6f.txt
txt: txt/cord-322516-wekvet6f.txt
title:
  Ceramide Accumulation Uncovers a Cycling Pathway for the cis-Golgi Network Marker, Infectious Bronchitis Virus M Protein

summary:
  We propose two models to explain the redistribution of IBV M protein induced by PDMP. Treatment of infected cells with lower concentrations of PDMP had no effect on IBV M localization and very little effect on SM synthesis (data not shown).


id: cord-289710-ucguzgdm
date: 1992-12-02
extension: .txt
mime: text/plain
words: 8024
sentence: 275
flesch: 43
cache: cache/cord-289710-ucguzgdm.txt
txt: txt/cord-289710-ucguzgdm.txt
title:
  Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane

summary:
  This surprising result raises the possibility that the vacuole is the default destination for yeast Golgi membrane proteins. This unexpected finding is discussed in terms of models involving either the vacuole as a default destination for membrane proteins, or by endocytosis to the vacuole following their default localization to the plasma membrane.


id: cord-323331-80d01l6f
author: Li, Jie
title: Golgi Structure and Function in Health, Stress, and Diseases
date: 2019-01-01
extension: .txt
mime: text/plain
words: 13524
sentence: 423
flesch: 33
cache: cache/cord-323331-80d01l6f.txt
txt: txt/cord-323331-80d01l6f.txt
summary:
  Interestingly, αSNAP depletion induces apoptosis independent of the cleavage of Golgi proteins such as GRASP65, golgin-160, and p115 but rather by dysregulation of ER-Golgi vesicle cycling and possibly through ER stress (Naydenov et al. 2012) . The Golgi matrix protein giantin is required for normal cilia function in zebrafish Role of phosphatidylinositol 4-phosphate (PI4P) and its binding protein GOLPH3 in hepatitis C virus secretion Selective export of human GPI-anchored proteins from the endoplasmic reticulum Membrane targeting of p115 phosphorylation mutants and their effects on Golgi integrity and secretory traffic Characterization of PSKH1, a novel human protein serine kinase with centrosomal, golgi, and nuclear localization Mutants of the protein serine kinase PSKH1 disassemble the Golgi apparatus Involvement of Golgin-160 in cell surface transport of renal ROMK channel: co-expression of Golgin-160 increases ROMK currents Rab and Arl GTPase family members cooperate in the localization of the golgin GCC185 The GOLPH3 pathway regulates Golgi shape and function and is activated by DNA damage Fragmentation and dispersal of Golgi proteins and redistribution of glycoproteins and glycolipids processed through the Golgi apparatus after infection with herpes simplex virus 1 The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis Making the Auroras glow: regulation of Aurora A and B kinase function by interacting proteins Aurrand-Lions M (2017) Genetic, structural, and chemical insights into the dual function of GRASP55 in germ cell Golgi remodeling and JAM-C polarized localization during spermatogenesis JNK2 controls fragmentation of the Golgi complex and the G2/M transition through phosphorylation of GRASP65 Thyroid hormone, T3-dependent phosphorylation and translocation of Trip230 from the Golgi complex to the nucleus Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration P21-activated protein kinase is overexpressed in hepatocellular carcinoma and enhances cancer metastasis involving c-Jun NH2-terminal kinase activation and paxillin phosphorylation A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis ZFPL1, a novel ring finger protein required for cis-Golgi integrity and efficient ER-to-Golgi transport Adhesion of Golgi cisternae by proteinaceous interactions: intercisternal bridges as putative adhesive structures The Golgi mitotic checkpoint is controlled by BARS-dependent fission of the Golgi ribbon into separate stacks in G2 Rab1A over-expression prevents Golgi apparatus fragmentation and partially corrects motor deficits in an alpha-synuclein based rat model of Parkinson's disease Identification and characterization of two novel (neuro)endocrine long coiled-coil proteins HACE1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1) GRASP65 and GRASP55 sequentially promote the transport of C-terminal valine-bearing cargos to and through the Golgi complex DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage Rab18 and Rab43 have key roles in ER-Golgi trafficking The endosomal pathway and the Golgi complex are involved in the infectious bursal disease virus life cycle Pentameric assembly of potassium channel tetramerization domain-containing protein 5 New insights into membrane trafficking and protein sorting The trans-Golgi network golgin, GCC185, is required for endosome-to-Golgi transport and maintenance of Golgi structure The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner GOLPH3 bridges phosphatidylinositol-4-phosphate and actomyosin to stretch and shape the Golgi to promote budding Phosphorylation of the vesicletethering protein p115 by a casein kinase II-like enzyme is required for Golgi reassembly from isolated mitotic fragments A general amphipathic alpha-helical motif for sensing membrane curvature Asymmetric tethering of flat and curved lipid membranes by a golgin Autophagy-based unconventional secretory pathway for extracellular delivery of IL-1beta The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network How accelerated Golgi trafficking may drive Alzheimer's disease (comment on Human autoantibodies to a novel Golgi protein golgin-67: high similarity with golgin-95/gm 130 autoantigen DNA damage triggers Golgi dispersal via DNA-PK and GOLPH3 Signalling to and from the secretory pathway GRASP55


id: cord-264468-3oxzzxnd
author: Salcedo, Suzana P.
title: SseG, a virulence protein that targets Salmonella to the Golgi network
date: 2003-10-01
extension: .txt
mime: text/plain
words: 6939
sentence: 279
flesch: 44
cache: cache/cord-264468-3oxzzxnd.txt
txt: txt/cord-264468-3oxzzxnd.txt
summary:
  We show here that the majority of SCVs become surrounded by Golgi membranes 4 h after invasion of epithelial cells, a process that is dependent on the SPI-2 TTSS effector SseG. Mutational analysis of this protein identi®ed a Golgi-targeting domain and de®ned further regions that are required for recruitment of SCVs to the Golgi network. Confocal X/Z reconstructions revealed that these microcolonies were partially or completely enveloped by Golgi membranes ( Figure 1A ; Supplementary ®gure S1 available at The EMBO Journal Online).


id: cord-329553-93tbgn2b
date: 1992-04-02
extension: .txt
mime: text/plain
words: 8076
sentence: 308
flesch: 48
cache: cache/cord-329553-93tbgn2b.txt
txt: txt/cord-329553-93tbgn2b.txt
title:
  The 17-residue transmembrane domain of beta-galactoside alpha 2,6- sialyltransferase is sufficient for Golgi retention

summary:
  Further experiments also showed that a 41--43 kD fragment of ST could be released from Golgi membranes by endogenous cathepsin D-like protease (Lammers and Jamieson, 1988; Weinstein et al ., 1987) . We found that the 17-residue transmembrane domain of ST is sufficient for Golgi localization and that this se-Native and Chimeric cDNA DPPIV.


id: cord-309384-vlk8cebh
author: Kolter, Thomas
title: Ganglioside Biochemistry
date: 2012-12-19
extension: .txt
mime: text/plain
words: 16942
sentence: 681
flesch: 31
cache: cache/cord-309384-vlk8cebh.txt
txt: txt/cord-309384-vlk8cebh.txt
summary:
  I integral membrane protein of the endoplasmic reticulum Zebrafish and mouse α2,3-sialyltransferases responsible for synthesizing GM4 ganglioside Prevention of experimental allergic encephalomyelitis by ganglioside G(M4) Purification and characterization of UDP-glucose:ceramide glucosyltransferase from rat liver Golgi membranes Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis Oligomerization and topology of the Golgi 28 ISRN Biochemistry membrane protein glucosylceramide synthase Identification of active site residues in glucosylceramide synthase: a nucleotide-binding/catalytic motif conserved with processive β-glycosyltransferases Topology of glucosylceramide synthesis in Golgi membranes from porcine submaxillary glands Lactosylceramide is synthesized in the lumen of the Golgi apparatus MDR1 Pglycoprotein is a lipid translocase of broad specificity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine ABC lipid transporters: extruders, flippases, or flopless activators? Pre-and post-Golgi translocation of glucosylceramide in glycosphingolipid synthesis Reconstitution of glucosylceramide flip-flop across endoplasmic reticulum: implications for mechanism of glycosphingolipid biosynthesis Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide Mutation of βglucosidase 2 causes glycolipid storage disease and impaired male fertility Biosynthesis of glycolipids cDNA cloning and expression of human lactosylceramide synthase Isolation and characterization of extremely minor gangliosides, G(M1b) and G(D1α), in adult bovine brains as developmentally regulated antigens Enhanced insulin sensitivity in mice lacking ganglioside GM3 Tissue-specific expression of cseries gangliosides in the extraneural system Structural characterization of a novel cholinergic neuron-specific ganglioside in bovine brain Biosynthetic pathway for a new series of gangliosides, GT1aα and GQ1bα Biosynthesis of the major brain gangliosides GD1a and GT1b Glycosphingolipid functions: insights from engineered mouse models A mouse B16 melanoma mutant deficient in glycolipids Complex gangliosides are essential in spermatogenesis of mice: possible roles in the transport of testosterone Mice lacking complex gangliosides develop Wallerian degeneration and myelination defects A functional role for complex gangliosides: motor deficits in GM2/GD2 synthase knockout mice Attenuation of interleukin 2 signal in the spleen cells of complex gangliosidelacking mice CD4 and CD8 T cells require different membrane gangliosides for activation Mice lacking major brain gangliosides develop parkinsonism G(M1) ganglioside rescues substantia nigra pars compacta neurons and increases dopamine synthesis in residual nigrostriatal dopaminergic neurons in MPTP-treated mice GM1 specifically interacts with α-synuclein and inhibits fibrillation Mice expressing only monosialoganglioside GM3 exhibit lethal audiogenic seizures Interruption of ganglioside synthesis produces central nervous system degeneration and altered axon-glial interactions Sphingolipid synthetic pathways are major regulators of lipid homeostasis Membranes in balance: mechanisms of sphingolipid homeostasis Regulation of ganglioside biosynthesis in the nervous system The cytoplasmic tail of GM3 synthase defines its subcellular localization, stability, and in vivo activity Multi-enzyme kinetic analysis of glycolipid biosynthesis Golgi localization of glycosyltransferases involved in ganglioside biosynthesis Regulation of ganglioside biosynthesis by enzyme complex formation of glycosyltransferases The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion Glycosyltransferase complexes improve glycolipid synthesis Physical and functional association of glycolipid N-acetylgalactosaminyl and galactosyl transferases in the Golgi apparatus Ganglioside glycosyltransferases organize in distinct multienzyme complexes in CHO-K1 cells Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains Membrane sialidase NEU3 is highly expressed in human melanoma cells promoting cell growth with minimal changes in the composition of gangliosides Sialidase occurs in both membranes of the nuclear envelope and hydrolyzes endogenous GD1a Degradation of glycolipids Lysosomal degradation of membrane lipids Activator proteins and topology of lysosomal sphingolipid catabolism Regulation of apoptosis-associated lysosomal membrane permeabilization Recycling compartments and the internal vesicles of multivesicular bodies harbor most of the cholesterol found in the endocytic pathway Biological function of the cellular lipid BMP-BMP as a key activator for cholesterol sorting and membrane digestion Metabolism, function and mass spectrometric analysis of bis(monoacylglycero)phosphate and cardiolipin Separation and characterization of late endosomal membrane domains Lysosome biogenesis and lysosomal membrane proteins: trafficking meets function Lysosomal membrane proteins: life between acid and neutral conditions Hoppe-Seyler's Zeitschrift für Physiologische Chemie The human G(M2) activator protein. Secondary storage of gangliosides GM2 and GM3 occurs also in Hurler disease [290] (mucopolysaccharidosis type I; α-Liduronidase deficiency).


id: cord-355580-4pv1zu1g
date: 1992-06-01
extension: .txt
mime: text/plain
words: 8419
sentence: 233
flesch: 35
cache: cache/cord-355580-4pv1zu1g.txt
txt: txt/cord-355580-4pv1zu1g.txt
title:
  O-glycosylation of intact and truncated ribophorins in brefeldin A- treated cells: newly synthesized intact ribophorins are only transiently accessible to the relocated glycosyltransferases

summary:
  Since pulse-labeling experiments with [35S]methionlne in BFAtreated cells (Fig. 1) showed that a substantial proportion of the newly synthesized intact ribophorin I molecules is modified during the subsequent chase, the lack of detectable incorporation of radioactive sugars into intact ribophorin I indicates that only newly synthesized, and not preexisting ribophorin molecules, can serve as a substrate for the O-glycosylation system. In contrast to the situation with [35S]methionlne, sugar precursors were only incorporated into the slower migrating forms of truncated ribophorin molecules that are characteristic of BFAtreated cells (Fig. 3, lanes b and c) .


id: cord-257754-pqxkyg8z
author: Reggiori, Fulvio
title: Membrane Origin for Autophagy
date: 2006-07-21
extension: .txt
mime: text/plain
words: 9237
sentence: 280
flesch: 32
cache: cache/cord-257754-pqxkyg8z.txt
txt: txt/cord-257754-pqxkyg8z.txt
summary:
  This notion is corroborated by the fact that the totality of Atg9 is associated with membranes Reggiori et al., 2005b) . Nice et al., 2002; Reggiori et al., 2003 Reggiori et al., , 2004a .


id: cord-022499-7d58f1k3
author: Mall, Sanjay
title: Transmembrane α helices
date: 2004-01-07
extension: .txt
mime: text/plain
words: 12227
sentence: 399
flesch: 45
cache: cache/cord-022499-7d58f1k3.txt
txt: txt/cord-022499-7d58f1k3.txt
summary:
  A spin-label study of lipid-protein interactions A mutant cytochrome b5 with a lengthened membrane anchor escapes from the endoplasmic reticulum and reaches the plasma membrane Hydrophobic mismatch and long-range protein/lipid interactions in bacteriorhodopsin/phosphatidylcholine vesicles Fluorescence quenching and electron spin resonance study of percolation in a two-phase lipid bilayer containing bacteriorhodopsin Transmembrane domain-dependent sorting of proteins to the ER and plasma membrane in yeast Membrane protein structure and stability: Implications of the first crystallographic analyses Control of the transmembrane orientation and interhelical interactions within membranes by hydrophobic helix length Hydrophobicity of the peptide C=O. • -H--N hydrogen bonded group Subcellular organization of glycosylation in mammalian cells Detergent structure in crystals of a bacterial photosynthetic reaction centre Structure of the detergent phase and proteindetergent interactions in crystals of the wild-type (Strain Y) Rhodobacter sphaeroides photochemical reaction center Molecular dynamics of Pfl coat protein in a phospholipid bilayer Conformational changes of phospholipid headgroups induced by a cationic integral membrane peptide as seen by deuterium magnetic resonance Influence of the intrinsic membrane protein bacteriorhodopsin on gel-phase domain topology in two-component phase-separated bilayers Transmembrane helix structure, dynamics, and interactions: Multi-nanosecond molecular dynamics simulations Annular and non-annular binding sites on the (Ca 2+ + Mg2+)-ATPase Interactions of cholesterol hemisuccinate with phospholipids and (Ca2+-Mg2+)-ATPase Functional rafts in cell membranes Mutational analysis of the signal-anchor domain of influenza virus neuraminidase Dependence of lipid membrane phase u'ansition temperature on the mismatch of protein and lipid hydrophobic thickness Lipid enrichment and selectivity of integral membrane proteins in two-component lipid bilayers Characterization of the single Ca 2+ binding site on the Ca2+-ATPase reconstituted with short and long chain phosphatidylcholines Molecular organization and dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine bilayers containing a transmembrane alpha-helical peptide A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6/~ resolution Molecular interactions between lecithin and sphingomyelin Principles of membrane protein assembly and structure Architecture of helix bundle membrane proteins: An analysis of cytochrome c oxidase from bovine mitochondria Hydrophobic mismatch and the incorporation of peptides into lipid bilayers: A possible mechanism for retention in the Golgi Experimentally determined bydrophobicity scale for proteins at membrane interfaces Molecular dynamics simulations of individual alpha-helices of bacteriorhodopsin in dimyristoylphosphatidylcholine. Whereas ESR spectra of spin-labeled lipids in the presence of membrane proteins typically show two-component spectra, as described above, ESR spectra for lipid bilayers containing the peptide L24 and for a tryptophan-containing peptide of the type AW2(LA)nW2A are single-component (de Planque et aL, 1998; Subczynski et al., 1998) .


id: cord-022354-aqtceqqo
author: HUNTER, ERIC
title: Membrane Insertion and Transport of Viral Glycoproteins: A Mutational Analysis
date: 2012-12-02
extension: .txt
mime: text/plain
words: 17775
sentence: 483
flesch: 32
cache: cache/cord-022354-aqtceqqo.txt
txt: txt/cord-022354-aqtceqqo.txt
summary:
  Perlman and Halvorson (1983) and von Heijne (1983) have examined sequences of a number of membrane proteins and have described amino acid sequence patterns that allow prediction of signal peptidase cleavage sites with greater than 90% accuracy. Many cell surface and membrane proteins of animal viruses are bound to the lipid bilayer by a membrane-spanning hydrophobic peptide close to the carboxy terminus of the polypeptide (reviewed by Warren, 1981; Armstrong et aL, 1981) .


id: cord-104239-xxlcdbqi
title: The organization of endoplasmic reticulum export complexes
date: 1996-10-01
extension: .txt
mime: text/plain
words: 11318
sentence: 388
flesch: 45
cache: cache/cord-104239-xxlcdbqi.txt
txt: txt/cord-104239-xxlcdbqi.txt
summary:
  We find that ER buds in vivo are nonrandomly distributed, being concentrated in regional foci we refer to as export complexes. We find that ER buds in vivo are nonrandomly distributed, being concentrated in regional foci we refer to as export complexes.


id: cord-269011-230p8rsf
author: de Haan, Cornelis A.M.
title: Molecular Interactions in the Assembly of Coronaviruses
date: 2005-08-31
extension: .txt
mime: text/plain
words: 22995
sentence: 727
flesch: 36
cache: cache/cord-269011-230p8rsf.txt
txt: txt/cord-269011-230p8rsf.txt
summary:
  MHV M proteins carry a well-conserved SS(X)TTXXP sequence at their extreme amino terminus. As the expression of these enzymes varies in cells, conservation of the SS(X)TTXXP motif in MHV M protein may serve to increase opportunities for the protein to become glycosylated in different cell types.


id: cord-253466-7gpije5d
author: Netherton, Christopher
date: 2007-08-31
extension: .txt
mime: text/plain
words: 26447
sentence: 827
flesch: 32
cache: cache/cord-253466-7gpije5d.txt
txt: txt/cord-253466-7gpije5d.txt
title:
  A Guide to Viral Inclusions, Membrane Rearrangements, Factories, and Viroplasm Produced During Virus Replication

summary:
  Ultrastructural studies and sequential development of virus isolated from normal and tumor tissue Acidic C terminus of vaccinia virus DNAbinding protein interacts with ribonucleotide reductase Formation of DNA replication structures in herpes virus-infected cells requires a viral DNA binding protein Comparison of the intranuclear distributions of herpes simplex virus proteins involved in various viral functions Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors MHC I-dependent antigen presentation is inhibited by poliovirus protein 3A Autophagy in innate and adaptive immunity Poliovirus 3A protein limits interleukin-6 (IL-6), IL-8, and beta interferon secretion during viral infection Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A Inhibition of endoplasmic reticulum-to-Golgi traffic by poliovirus protein 3A: These initially appear close to the nuclear envelope, possibly at sites where the genome first enters the nucleus following capsid disassembly at nuclear pores (Everett and Murray, 2005) , and are then seen throughout the nucleus (Everett et al., 2004) .


# ent

id: cord-026010-61j07gq3
sid: 37
eid: 1
type: GPE
entity:
  ððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððððð


id: cord-309384-vlk8cebh
sid: 574
eid: 2
type: ORG
entity:
  der phosphatide und anderer lipide des gehirns und der leber bei der niemann-pick'schen krankheit über die ganglioside des gehirns bei


